CN1686168A - Anticoid factor activator compound injection agent capable of treating arthritis - Google Patents
Anticoid factor activator compound injection agent capable of treating arthritis Download PDFInfo
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- CN1686168A CN1686168A CNA2005100336403A CN200510033640A CN1686168A CN 1686168 A CN1686168 A CN 1686168A CN A2005100336403 A CNA2005100336403 A CN A2005100336403A CN 200510033640 A CN200510033640 A CN 200510033640A CN 1686168 A CN1686168 A CN 1686168A
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Abstract
A cold-resistant factor activator in the form of injection for treating arthritis is prepared from the fresh joint's bone tissue of cold-resistant animals through pulverizing, cold storage, extracting and enzymolyzing.
Description
Technical field:
The present invention relates to a kind of biological preparation, be specifically related to a kind of arthritic Anticoid factor activator compound injection agent of cold and cool type for the treatment of.
Background technology:
Arthritis is commonly encountered diseases, frequently-occurring disease, and the whole world has 3.5 hundred million approximately.Arthritic kind is a lot, and in the majority to be afraid of cold and cool arthritis.Domestic medical circle rheumatism expert northeastward, the survey showed that in the Beijing area: have being grown up by investigation of 33%-77% to suffer from " rheumatic arthralgia " (promptly old cold disease, benign arthritis) approximately; South hot day, sickness rate also was on the increase owing to use air-conditioning, electric fan etc.; Simultaneously, with advancing age, prevalence is also increasing, and is in general, cold relatively higher with relative humid region prevalence.Though the active drug of some different curative effects is arranged at present, still there is not the arthritic medicine of radical cure of generally acknowledging, it is said that in the recent period effect better has " closing Zhi Ling " (the containing cetoleic acid fat) of U.S.'s import, need 3-4 the course of treatment, expense is about 3150-4200 unit; About 1800 yuan of domestic drug " Portugal upright " (containing glucosamine) each course of treatment, and annually need repetitive therapy 2-3 time, bother very much.
Summary of the invention:
Technical problem to be solved by this invention is the defective that overcomes above-mentioned prior art, and a kind of human body cold tolerance, with low cost, Anticoid factor activator compound injection agent that therapeutic effect is remarkable of significantly improving is provided, and can treat because of being subjected to the arthritis due to cold and cool.
The present invention solves the problems of the technologies described above by following technical scheme.It is characterized in that it being that fresh ossa articularia with cold-resistant animal is organized as raw material, by the injection of pulverizing cold preservation, comprehensively extracting, enzymatic degradation is made;
Described cold-resistant animal can be dog class animal, goose, Lepus timidus, Bears, Capreolus capreolus (Linnaeus), deer or Chinese forest frog;
Its concrete steps are:
1) pulverizes cold preservation: get the fresh bone joint tissue of cold-resistant animal, clean, sterilize, remove the peel hair fat, cold preservation after pulverizing;
2) the comprehensive extraction: the tissue after the cold preservation is added the water for injection that 1-2 doubly measures, and the dipping after-filtration is got filtrate and is put in addition; Add 30% ethanol that 1-2 doubly measures in its filtering residue, remove ethanol behind the immersion filtration, get filtrate and put in addition; Add the sodium hydroxide solution that 1-2 doubly measures again in its filtering residue, the dipping after-filtration is transferred pH value 6-8, gets filtrate and puts in addition; In its filtering residue, add 1-2 times of water gaging then and decoct again, get filtrate; Above-mentioned four kinds of filtrates are merged the back transfer pH value 2-3;
3) enzymatic degradation: in above-mentioned amalgamation liquid, add concentration and be 0.5-2%, 1: 3000 pepsin degraded, transfer pH value to 6-8, or adopt the pancreatin degraded of 0.5-2%, transfer pH value to 8-10;
4) degraded back solution concentration transfers to 10%, filters the back packing with biofilter then; Or filter with the G4 ball earlier, under 100 ℃ of temperature, sterilized 30 minutes after the packing.
In the above-mentioned steps: described cold preservation time is more than 20-24 hour; Described dip time is 3-5 hour; Described concentration of sodium hydroxide solution is 0.2-1%; Described pepsin or pancreatin degradation time are 7-9 hour, and temperature is 30 ℃-45 ℃.
The present invention has following technique effect:
1, the present invention is by extracting contained cold-resistant factor in the cold-resistant fresh ossa articularia tissue of animal body, through biochemical treatment, the comprehensive extraction injected human body after making injection, but cold-resistant factor, enzyme or the short enzyme of human activin cold-resistant system concealment itself or degeneration, make because of taken a turn for the worse by the cold and cool tissue antigen that makes a variation, make the identification of T lymphocyte no longer be considered as the dissident, and no longer instruct macrophage to attack it, blocked pathological lesion, the joint fricative is wiped sense and is obviously alleviated, and disease is decreased obtain repairing;
2, contained active substance among the present invention, can produce the material of " energy metabolism bypass " equal quality of high energy, can produce more energy, muscle power strengthens, cold tolerance also significantly strengthens, generation is beneficial to the joint and changes--the logical microcirculation of temperature, and remove the part and cause the pain factor, effectively alleviate or eliminates arthritis and keenly feel;
3, the present invention also can provide linked groups to repair required important substance, and acceleration of tissue repair is had wholesome effects and decreased the rehabilitation of tissue, and treatment time is short, and is evident in efficacy;
4, the present invention is a biological preparation, and manufacture method is simple,, with low cost, safety non-toxic does not have irritated, without any side effects.
The specific embodiment:
" wind and cold wet close and be numbness ", " cold be numbness head ".Overwhelming majority arthritic all has wind-cold damp pathogen invasion and attack history, because wind, cold, wet effect, cause the inherent Developmental and Metabolic Disorder of connective tissue in the human body, and the shallow table blood fortune of joint tissue is relatively poor, cold can make peripheral vessels shrink, more influence the blood supply of joint tissue and the drainage of metabolite, and be subject to extraneous wind and cold, the wet invasion and attack and the air pressure of weather, the influence that ionization degree etc. change, when external condition makes the human body portion gene, cell, when tissue morphs, can activate the T lymphocyte, the instruction macrophage, engulf, remove, destroy the normal equilibrium of organismic internal environment.Element is asked and said: husband's five or eight kidney qis decline ..., decline of the fire from the gate of life, YANG QI deficiency, then the extremity bones of the body collectively loses in yang-energy warmly, and human or animal's thymus promptly begins to degenerate to sexual maturity, the atrophy gradually with the growth at age.Cell tissue is aging, and immune surveillance recognition system ability weakens and easily disorderly, and under the stimulation that arthrosis is decreased repeatedly, the recognition of nonself that the T lymphocyte can not be correct, the T lymphocyte of some misleadings irritate macrophage, destroys autologous tissue and accidentally injure.When the people in a certain a certain amount of time and place, during cause influences such as wind-engaging cold-damp, the T lymphocyte is activated, and constantly instructs macrophage to be regardless of the dissident, sick cell tissue and natural joint tissue is engulfed, is corroded, and cause joint tissue damage and inflammation.In addition owing to human initiatively conform-warm clothing, room, make body " cold-resistant system " some factor or enzyme is degenerated or concealment, thereby reduced defensive ability/resistance ability to cold-damp etc.
Biology survival of the fittest in natural environment, uncomfortable person then is eliminated, and its evolution with physiology, biochemistry, organizational structure is associated.The psychrotolerant of extremely frigid zones, why can in the natural environment of severe cold, survive loftily, be because of in its cold adaptation, there being " cold-resistant factor " or " cold-resistant factor activin ", enzyme or short enzyme retain or evolve, make its physiology, biochemical reaction to cold have special reaction and (or) energy, merit, hot high energy metabolic way, make cold adaptation more become to improving and strengthen, and guarantee that it survives in cold environment.Simultaneously, the rheumatism factor such as the removing wind and cold that its " cold-resistant system " and " rheumatism immune system " can be correct, instant is wet and cell, tissue that the disease that causes is decreased, and do not accidentally injure self-normal cell, tissue etc., it is normal to keep function of organization; Enzyme system still keeps normal active under severe cold condition, special energy metabolism, and the biochemical reaction of generation high energy is survived in the environment of severe cold to suit.
Various organizing respectively has special organizational structure and function, it is associated with special molecular structure, the performance of molecule depend on determinant and (or) certain element and present molecular biology characteristics, it has the tropism of having a liking for special molecular to constituting special organizational structure, as: iodine is had a liking for thyroid, and selenium is to action of the heart etc.The cold-resistant animal of extremely frigid zones has higher tolerance to cold, the present invention is according to cold and cool property arthritis pathogenic factor, adopt the fresh ossa articularia tissue of cold-resistant animal, through biochemical treatment, the comprehensive micromolecule polypeptide that keeps its determinant that extracts, aminoacid isoreactivity composition and cold-resistant factor, make in the compound injection effect on human body, can activate and improve the cold tolerance of human body, promote the blood circulation of patient part, replenish, the reparation in reinforcement and joint, activate the antigen of reverse variation etc., thoroughly repair pathological changes joint and surrounding soft tissue thereof, recover its normal physiological function, chop the modified reaction chain that forms arthralgia pain off, reach the purpose of the interior heresy of eradicating rheumatic ostalgia.
Impaired T lymphocyte in the scalable human body of the present invention " rheumatism immune system ", recover, correct and T lymphocyte that control misleads, the cell of instruction macrophage phagocytic variation, tissue etc., and not injuring normal cell, tissue, recover normal recognition function, stop the arthritis damage.Pathological changes is eliminated gradually, replaced by normal structure, impaired joint function disturbance recovers gradually, thereby realizes permanent rehabilitation, has stablized the untoward reaction that is subjected to extraneous negative effect simultaneously, as weakening, disappearing Changes in weather premonition.
The present invention is that the fresh ossa articularia with cold-resistant animal is organized as raw material, make injection by pulverizing cold preservation, comprehensive extraction, enzymatic degradation, wherein said cold-resistant animal is dog class animal, goose, Lepus timidus, Bears, Capreolus capreolus (Linnaeus), deer or Chinese forest frog, these cold-resistant animals have stronger tolerance to cold, on Changbai Mountain of China, high and cold area existence such as Daxing'an Mountainrange.Concrete enforcement comprises the steps:
1) pulverize cold preservation: get the fresh ossa articularia tissue of cold-resistant animal, clean, fur fat is removed in sterilization, cuts into chunks, and pulverizes back cold preservation more than 20-24 hour, can produce more cold-resistant factor after the cold preservation;
2) the comprehensive extraction: the tissue after the cold preservation is added the water for injection that 1-2 doubly measures, flood 3-5 hour after-filtration, form filtrate 1., in its filtering residue, add 1-2 then and doubly measure 30% ethanol, also flooded 3-5 hour, cross elimination ethanol, form filtrate 2., in its filtering residue, add 1-2 again and doubly measure the sodium hydroxide solution that concentration is 0.2-1%, flood 3-5 hour after-filtration, form filtrate 3., 3. this filtrate must transfer pH value 6-8, avoids the influence of alkalescence to other compositions; Again its filtering residue is added water 1-2 times of water gaging again and decoct, filter the back and form filtrate 4., merge above-mentioned four filtrates, transfer pH value 2-3;
3) enzymatic degradation: pepsin (1: the 3000) degraded that in above-mentioned amalgamation liquid, adds 0.5-2%, degradation time is 7-9 hour, temperature is 30 ℃-45 ℃, transfer pH value to 6-8 then: or adopt the pancreatin degraded of 0.5-2%, degradation time also is 7-9 hour, temperature is 30 ℃-45 ℃, transfers pH value to 8-10 then;
4) degraded back solution concentration transfers to 10%, filters with biofilter then, to remove precipitation and slag, is distributed into finished product again; Or earlier with G4 ball filter and remove residue, finished product was sterilized 30 minutes under 100 ℃ of temperature after the packing.
One, zoopery:
1, cold-resistant experiment: every group of 5 white mice are placed the outdoor of nature cold conditions, observe by eating condition at ordinary times, the result is:
1) preliminary experiment group:
White mice was freezed to death in the time of-14 ℃ in 1 hour, was freezed to death in about 50 minutes in the time of-18 ℃;
2) experimental group:
" normal saline group ": injection has the white mice of normal saline all to freeze to death in 32 hours, and minimum temperature is-16 ℃;
" dexamethasone " group: injection has the white mice of dexamethasone all to freeze to death in 38 hours, minimum temperature-19.3 ℃;
And injection has the present invention's's (product of the present invention uses title: " joint spirit pin ") white mice all to freeze to death in 331 hours, and when first white mice freezed to death in beginning in 41 hours, temperature was-21 ℃ at that time.
The zoopery explanation, experimental group of the present invention can significantly improve the cold tolerance of white mice.
2, acute toxicity testing:
Get 15 of white mice (average weight 35.4 gram), the present invention is injected its tail vein for 0.5 milliliter, remove one because of the inject time of some atrophy at that time too soon (changing over to normal after a while), all the other enliven as usual, and are still normal after 48 hours.
Calculate: become body weight for humans by 50 kilograms of calculating, white mice 35.4 grams, the weight of promptly being grown up: white mice weight=1416: 1.
The adult is by 2 milliliters/time, and the dosis tolerata of white mice is more than 348.7 times of consumption of adult;
The adult is by 4 milliliters/time, and the dosis tolerata of white mice is more than 176 times of consumption of adult.It is then safer more than 60 times to be equivalent to people's consumption in accordance with regulations, this dosis tolerata be 176-348 doubly more than, and clinical be intramuscular injection, and zoopery is intravenous injection, so the present invention is very safe.
3, irritant experiment:
Get two of rabbit, with 1 milliliter of the present invention, 1 milliliter of normal saline, inject quadriceps femoris place, the left and right sides respectively, 48 heads shoot dead, dissect the both sides quadriceps femoris and do not see inflammatory reaction and hemorrhage, so can make local injection.
4, hemolytic experiment:
Get 10 milliliters of Sanguis Leporis seu oryctolagi, defibrinate, cyclic washing three times, get 1 milliliter in blood cell, add 40 milliliters in 0.9% sodium chloride, form 2% erythrocyte mixed liquor, divide and get 2.5 milliliters in vitro in four, add 0.1 milliliter of the present invention (content 2%), 0.3 milliliter (content 6%), 0.5 milliliter (content 10%) respectively within it, surplus usefulness 0.9% sodium chloride is mended to 5 milliliters of (promptly 2.4 milliliters, 2.2 milliliters, 2.0 milliliters, 2.5 milliliters) mixings suspension that takes on a red color and is put 28 ℃ of room temperatures and place observations after a hour, no haemolysis.
Two: chemical analysis:
1, the present invention tests through ultraviolet spectrometry, and its value is:
230nm????OD????11.88
260nm????OD????2.145
280nm????OD????1.54
2, through the experiment of polypropylene protein electrophoresis, only there is single district band in the 4S district;
3, be 0.3735% through deciding its total nitrogen of nitrogen measuring.
4, amino acid analysis laboratory report:
Sample concentration: be diluted to 1.5% solution
Analytical tool: KLA-5 type amino-acid analyzer
Analysis condition: 6 φ-130-PH-2.3-SS
| Aminoacid | ??H | ??W | The HW sample | The HW mark | Every ml sample size (mg amount) |
| Lysine (Lys) | ??4 | ??16.8 | ??67.2 | ??114.1 | ??0.0538 |
| Histidine (His) | ??0.75 | ??22.6 | ??16.95 | ??105.05 | ??0.0169 |
| Free ammonia (Amm) | ??19.1 | ??22.8 | ??435.48 | ??88.44 | ??0.1317 |
| Arginine (Arg) | ??0.7 | ??29 | ??20.3 | ??99 | ??0.0216 |
| Aspartic acid (Acp) | ??4.25 | ??14.4 | ??61.2 | ??110.96 | ??0.0367 |
| Threonine (Thr) | ??2.5 | ??17.1 | ??42.75 | ??112.89 | ??0.0226 |
| Serine (Ser) | ??4.2 | ??16.5 | ??69.3 | ??117 | ??0.0311 |
| Glutamic acid (Glu) | ??3.7 | ??19.9 | ??73.63 | ??105.6 | ??0.0513 |
| Proline (Pro) | Trace | ||||
| Glycine (Gly) | ??4.8 | ??20.1 | ??96.48 | ??115.42 | ??0.0314 |
| Alanine (Ala) | ??4.1 | ??21.3 | ??87.33 | ??113.95 | ??0.0341 |
| Cysteine (Cys) | Do not have | ||||
| Valine (Val) | ??4.2 | ??12.3 | ??51.66 | ??119.84 | ??0.0252 |
| Methionine (Met) | ??1.2 | ??15.9 | ??19.08 | ??115.24 | ??0.0124 |
| Isoleucine (Ileu) | ??1.25 | ??16 | ??20 | ??115.02 | ??0.0114 |
| Leucine (Leu) | ??3 | ??17.6 | ??52.8 | ??118.3 | ??0.0293 |
| Tyrosine (Tyr) | ??0.8 | ??26.5 | ??21.2 | ??112.75 | ??0.0170 |
| Phenylalanine (Phe) | ??1.1 | ??34.5 | ??37.95 | ??111.34 | ??0.0282 |
| Total content | ??0.5547 |
Three, some cases:
Example 1: Liu XX, the man, 46 years old, cause was at underwater work in 7 years, be subjected to freezing 2 hours and cause ankle joint pain all the year round, limitation of activity is slow in action, and is afraid of cold, the change of weather had premonition in preceding 2-3 days, inspection is diagnosed as rheumatic arthralgia, does not imitate through treatment for many years, needs clothes analgesic respite throughout the year, 4 milliliters/time of back intramuscular injection the present invention, inject altogether 20 times, arthralgia disappears, and the ankle joint range of activity is more preceding to be increased to 42 ° gradually, strong flexibly, being afraid of cold obviously alleviates, and premonition goes down before the change of weather, and the local skin temperature of knee joint ankle joint is gone up 3.4 ℃.Pay a return visit after 2 years, the joint is pain more not, does not obey analgesic after the treatment again, and the more preceding increasing of joint motion is and strong, and premonition is not obvious before the change of weather.
Example 2: beam XX, man, 54 years old, extremities joint pain 7-8 needs through the informal dress analgesic, and the warm fearness of happiness is cool, need wear cotton-padded trousers summer, checks to be diagnosed as rheumatic arthralgia, 4 milliliters/time of back intramuscular injection the present invention, injected altogether 15 days, arthralgia disappears, and does not obey analgesic after the injection again, be afraid of frightened disappearing, taken off cotton-padded trousers summer, wear the clothes the same with ordinary person, follow up a case by regular visits to after 2 years, be as good as with ordinary person.
Example 3: Pan X, man, 43 years old, two knee joints are because of being subjected to cold pain 12 years, and it is limited to squat down, and are afraid of cool, need add hot day in summer and wear the autumn trousers, the informal dress analgesic is postponed separating, the knee joint fricative+, wipe sense +++, local skin relaxing the bowels with purgatives of warm nature falls, and is diagnosed as rheumatic arthralgia, 4 milliliters/time of back intramuscular injection the present invention, when injecting 10 days, pain obviously alleviates, when injecting 20 times, pain disappears, and the knee joint activity strengthens, and is not afraid of cold, the knee joint fricative, wiping sense obviously alleviates, follow up a case by regular visits to not pain again of knee joint after 18 months, the activity of squatting down is normal, is afraid of frightened the disappearance, knee joint fricative (±) is wiped sense (+) local skin temperature and is often become a full member, and the joint is pain more not.
Example 4: Lee XX, the woman, 40 years old, wet cool because of being subjected to, year surplus two gonalgia 7, very bitterly, need hold up stair downstairs, two legs and feet are afraid of cool, always wear the clothes in a season at ordinary times than the normal person, foot is lossless more, easily knots, and the change of weather had premonition in preceding 2-3 days, have a pain in the leg and increase the weight of, check to be diagnosed as rheumatic arthralgia, 4 milliliters/time of injection the present invention are when injecting 7 days, two legs and feet are afraid of cool the disappearance, and arthralgia alleviates, inject 20 times after, the joint is not bitterly, be not afraid of cold, foot is perspired, and knots and ends, and following up a case by regular visits in 3 years does not repeatedly have recurrence.
Example 5: tall X, the woman, 22 years old, surplus because of two gonalgia 8 years of suffering from cold, happiness is warm be afraid of cool, pain increases the weight of before the change of weather, check to be diagnosed as rheumatic arthralgia, and 3 milliliters/time of injection the present invention, when injecting 3 times, arthralgia disappears, be afraid of cool the disappearance, can wear skirt summer, followed up a case by regular visits to 1 year, and more bitterly, the change of weather does not have uncomfortable in the joint.
Example 6: Zheng XX, woman, 51 years old, whole body arthralgia 14 years, the postemphasis of getting involved, catch cold, left knee joint activity be pain then, squats down and upright difficulty, checks to be diagnosed as rheumatic arthralgia and hyperosteogeny, 2 milliliters/time of intramuscular injection the present invention, totally 15, each medication posterior joint pain relief, limb activity is strong.Follow up a case by regular visits to knee-joint pain after 1 year and disappear, movable normal, brisk.
Example 7: marquis XX, woman, 20 years old, wrist, elbow, knee joint, ankle joint wandering pain 1 year are surplus, redness and swelling of joints during pain, the dyskinesia, once obeyed prednisone, Phenylbutazone, red because of the joint, swollenly lift into the ward 2 milliliters of back intramuscular injection the present invention, every day 2 times, inject 9 days (simultaneously in addition intramuscular injection penicillin 7 days) altogether, arthralgia disappears, and movable normal, injection 3 days is continued in the back.Following up a case by regular visits to the joint after 1 year does not swell and ache again.
Example 8: open XX, the woman, 45 years old, wrist, knee joint, ankle joint wandering pain 3 years were surplus, and limbs are heavy, increase the weight of before the change of weather, right knee joint fricative+.4 milliliters of intramuscular injection the present invention, every day 1 time, intramuscular injection after 10 days two lower limb exercises flexible, pain disappears, inject 5 days again after, discomfort alleviates before the change of weather, injects 24 days posterior joints and disappears bitterly.Follow up a case by regular visits to after 2 years and do not use other antirheumatics, arthralgia and sense of heaviness disappear, and right knee joint fricative disappears, and flexibly movable, no weak, walking does not think tired for 8000 meters yet.
Example 9: Rhizoma Zingiberis Recens XX, the woman, 50 years old, right shoulder joint have a cold the back pain year surplus, the upper limb limitation of activity can not be combed one's hair, and is diagnosed as scapulohumeral periarthritis, 3 milliliters of intramuscular injection the present invention annotate 8 times every day 1 time altogether, the shoulder joint activity is normal.Follow up a case by regular visits to after 1 year, shoulder joint is pain no longer, and is movable normal.
Claims (7)
1, a kind of anticoid factor activator compound injection agent capable for the treatment of arthritis is characterized in that it being that fresh ossa articularia with cold-resistant animal is organized as raw material, by the injection of pulverizing cold preservation, comprehensively extracting, enzymatic degradation is made.
2, anticoid factor activator compound injection agent capable for the treatment of arthritis according to claim 1 is characterized in that described cold-resistant animal is dog class animal, goose, Lepus timidus, Bears, Capreolus capreolus (Linnaeus), deer or Chinese forest frog.
3, anticoid factor activator compound injection agent capable for the treatment of arthritis according to claim 1 and 2 is characterized in that comprising following concrete steps:
1) pulverizes cold preservation: get the fresh bone joint tissue of cold-resistant animal, clean, sterilize, remove the peel hair fat, cold preservation after pulverizing;
2) the comprehensive extraction: the tissue after the cold preservation is added the water for injection that 1-2 doubly measures, and the dipping after-filtration is got filtrate and is put in addition; Add 30% ethanol that 1-2 doubly measures in its filtering residue, remove ethanol behind the immersion filtration, get filtrate and put in addition; Add the sodium hydroxide solution that 1-2 doubly measures again in its filtering residue, the dipping after-filtration is transferred pH value 6-8, gets filtrate and puts in addition; In its filtering residue, add 1-2 times of water gaging then and decoct again, get filtrate; Above-mentioned four kinds of filtrates are merged the back transfer pH value 2-3;
3) enzymatic degradation: in above-mentioned amalgamation liquid, add concentration and be 0.5-2%, 1: 3000 pepsin degraded, transfer pH value to 6-8, or adopt the pancreatin degraded of 0.5-2%, transfer pH value to 8-10;
4) degraded back solution concentration transfers to 10%, filters the back packing with biofilter then; Or filter with the G4 ball earlier, under 100 ℃ of temperature, sterilized 30 minutes after the packing.
4, anticoid factor activator compound injection agent capable for the treatment of arthritis according to claim 3 is characterized in that described cold preservation time is more than 20-24 hour.
5, anticoid factor activator compound injection agent capable for the treatment of arthritis according to claim 3 is characterized in that described dip time is 3-5 hour.
6, anticoid factor activator compound injection agent capable for the treatment of arthritis according to claim 3 is characterized in that described concentration of sodium hydroxide solution is 0.2-1%.
7, anticoid factor activator compound injection agent capable for the treatment of arthritis according to claim 3 is characterized in that described pepsin or pancreatin degradation time are 7-9 hour, and temperature is 30 ℃-45 ℃.
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| CNA2005100336403A CN1686168A (en) | 2005-03-21 | 2005-03-21 | Anticoid factor activator compound injection agent capable of treating arthritis |
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| CNA2005100336403A CN1686168A (en) | 2005-03-21 | 2005-03-21 | Anticoid factor activator compound injection agent capable of treating arthritis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113287756A (en) * | 2021-05-26 | 2021-08-24 | 东莞十度生物科技有限公司 | Active Chinese medicine source of compound active polypeptide series and its prepn |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113287756A (en) * | 2021-05-26 | 2021-08-24 | 东莞十度生物科技有限公司 | Active Chinese medicine source of compound active polypeptide series and its prepn |
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