CN1683390A - Method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed - Google Patents
Method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed Download PDFInfo
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- CN1683390A CN1683390A CN 200510018390 CN200510018390A CN1683390A CN 1683390 A CN1683390 A CN 1683390A CN 200510018390 CN200510018390 CN 200510018390 CN 200510018390 A CN200510018390 A CN 200510018390A CN 1683390 A CN1683390 A CN 1683390A
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Abstract
The process of producing poisonless concentrated rape seed protein from cold pressed decoated rapeseed cake includes the following steps: two stage extraction with composite solvent comprising alcohol, water and added sodium hydroxide, solid-liquid separation of the extracted wet solid in automatic horizontal centrifuge, eliminating the residual alcohol in the solid with a vacuum low temperature solvent eliminator to obtain poisonless concentrated rape seed protein, recovering rape seed oil and alcohol from the extracted liquid and reusing alcohol through distillation. In the two extraction stages, the first one has higher alcohol concentration.
Description
Technical field
The invention belongs to Vegetable oil lipoprotein and vegetable-protein processing technique field.Be applicable to the deep development utilization of Semen Brassicae campestris (containing the double-low rapeseed seed) decortication cold pressed cake.Be specifically related to the method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed.
Background technology
Semen Brassicae campestris is the large oil crops of China, and annual production is more than 1,000 ten thousand tons, and the cake amount behind the squeezing system oil is about 6,000,000 tons.Rape seed protein is best in all vegetable-proteins, its amino acid balance is better than soybean protein, close with Food and Argriculture OrganizationFAO/world health organisation recommendations mode value, be a kind of full price albumen, its digestibility is 95%～100%, and it is 3.0～3.5 that albumen is tired, also higher than soybean protein, nutritive value is parity with or superiority over animal proteinum, is a resource treasure-house extremely leaved for development.But owing to contain toxicant in the Semen Brassicae campestris---sulphur glucoside and anti-nutrient substance phytate etc. are obstructed its Application and Development.As existing detoxification technology, conclude and to get up to mainly contain three major types: 1. physico-chemical process, as passivation mustard seed enzyme process, heat treating process, expanded detoxicity method, ultraviolet irradiation, microwave treatment method etc.; 2. chemical process, as the alkaline purification method, soda acid salt edman degradation Edman, aqua detoxicity method, solvent extraction method etc.; 3. biological method is as enzymatic hydrolysis method, microbe fermentation method etc.; The sulphur glucoside though these methods can partly remove or degrade, not thorough.Some method is better, as aqua method and SHMP method combined process (Guangzhou foodstuffs industry science and technology, 1998 the 3rd phases), aqueous acetone extraction (Chinese grain and oil journal the 4th phase of calendar year 2001), ether Ethanol Method (2004 the 3rd phases of Chinese grain and oil journal), but complex process, facility investment is big, energy consumption is high.All can not be as the effective ways of producing nontoxic rapeseed protein concentrate.And the raw material that these poison-removing methods use is rape seed, rapeseed cake or rapeseed meal, is that raw material is produced nontoxic rapeseed protein concentrate and do not appeared in the newspapers with vegetable seed decortication cold pressed cake.
Summary of the invention
Purpose of the present invention can realize by following technique measures, and the rapeseed peeling cold pressed cake is produced nontoxic rapeseed protein concentrate through the technological process of the double solvents extraction of two stages, solid-liquid separation, vacuum and low temperature precipitation.
Specifically, earlier with the aqueous ethanol of 85%～95% (v/v) and add the double solvents that the NaOH of aqueous ethanol consumption 0.05%～0.3% (w/v) forms, to be 70 ℃～80 ℃, decortication cold press rapeseed cake in extraction temperature be 1: 2～12 with the ratio (solid-to-liquid ratio) of solvent, carry out the fs leaching under the extraction times 3～8 times, extraction time 5～50min/ time condition; And then with the aqueous ethanol of 50%～80% (v/v) and add the double solvents of NaOH of 0.1%～0.3% (w/v) of aqueous ethanol consumption, be in extraction temperature that 40 ℃～70 ℃, solid-to-liquid ratio are 1: 2～10, carry out the subordinate phase extraction under the extraction times 3～10 times, extraction time 5～30min/ time condition.The mixed solution that two stages extraction back and centrifugation obtain all evaporates stripping, to reclaim grease and ethanol.The wet solid substance that obtains after the extraction is through centrifugation, and then promptly makes nontoxic rapeseed protein concentrate through vacuum-drying precipitation Ji, the ethanol of recovery metering proportion again after distillation, and circulation is used for extraction process.
The advantage that the present invention had:
1, detoxification efficiency is good, and the maximum toxicant sulphur glucoside in the rape seed protein can remove fully, and the phytic acid residual quantity is also within 1%.
2, the polarity of grease in the rapeseed cake and toxicant differs bigger, adopts that first concentration is higher, the lower aqueous ethanol of back concentration and adding assistant be as solvent, extraction stage by stage, not only economical effectiveness but also good.
3, energy consumption is low, behind the two-section extraction, adopts automatic horizontal centrifuge to carry out solid-liquid separation, isolates most ethanol, thereby has alleviated the load of vacuum and low temperature precipitation greatly, makes precipitation easier.
4, simple, the protein yield height of technology, three-waste free pollution are scientific and reasonable.
5, nontoxic rapeseed protein concentrate quality height can be used as the raw material of high-grade feed and foodstuffs industry.
Description of drawings
Accompanying drawing is technical process signal of the present invention
Embodiment
Embodiment further describes the present invention below in conjunction with accompanying drawing
Accompanying drawing is embodiments of the invention, and subordinate list is the decortication cold press rapeseed cake of the embodiment of the invention and the component content of prepared rapeseed protein concentrate.
Referring to accompanying drawing, the rapeseed peeling cold pressed cake enters batch extractor and carries out fs double solvents extraction, double solvents is the aqueous ethanol of 90% (v/v) and is added with the sodium hydroxide that accounts for aqueous ethanol content 0.1% (w/v), 76 ℃ of extraction temperature, solid-to-liquid ratio is 1: 10, extraction times 5 times extracted about 45 minutes time at every turn, and the mixing oil devaporation stripping that obtains after the extraction reclaims ethanol and makes the extraction crude oil.Solid substance after the extraction, carry out the extraction of subordinate phase double solvents with the aqueous ethanol that contains 70% (v/v) that account for aqueous ethanol content 0.2% (w/v) sodium hydroxide, about 50 ℃ of extraction temperature, solid-to-liquid ratio is 1: 8, extraction times 6 times, about each 20 minutes extraction time, the liquid that extraction obtains reclaims ethanol through the evaporation stripping, what obtain contains the wet solid substance of alcoholic acid, through whizzer (rotating speed 1500r/min) solid-liquid separation, liquid after the separation also devaporation stripping reclaims ethanol, and all reclaim ethanol and recycle after distillation.Isolated solid substance enters the vacuum and low temperature precipitation device and reclaims ethanol and dry, is rapeseed protein concentrate, and its recovery rate is about 64% of the decortication cold pressed cake.
The component content of subordinate list rapeseed peeling cold pressed cake and nontoxic rapeseed protein concentrate
| Composition | Decortication cold press rapeseed cake | Rapeseed protein concentrate |
| Oil length (butt), the % water ratio, the % ash content, the % robust fibre, % sulphur glucoside, the % phytic acid, % crude protein (butt), % | ????11.50 ????10.00 ????6.80 ????4.90 ????3.57 ????3.51 ????37.10 | 2.42 5.70 11.50 7.80 do not detect 0.72 57.20 |
Claims (4)
1, a kind of method of producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed, it is characterized in that the rapeseed peeling cold pressed cake is through fs double solvents extraction, the extraction of subordinate phase double solvents, solid-liquid centrifugation, vacuum and low temperature remove the technological process of solvent and produce nontoxic rapeseed protein concentrate.
2, the method for a kind of producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed according to claim 1 is characterized in that: the double solvents of fs extraction is made up of with the NaOH mixing that accounts for aqueous ethanol amount 0.05%～0.3% (w/v) the aqueous ethanol of 85%～95% (v/v); The double solvents of subordinate phase extraction is mixed with the NaOH that accounts for aqueous ethanol amount 0.1%～0.3% (w/v) by 50%～80% (v/v) aqueous ethanol and forms.
3, the method for according to claim a kind of producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed is characterized in that: the processing condition of fs extraction are: the ratio of 70 ℃～80 ℃ of temperature, cake and double solvents is 1: 2～12, time 5～50min/ time, number of times 3～8 times; The processing condition of subordinate phase extraction are: the ratio of 40 ℃～70 ℃ of temperature, cake and double solvents is 1: 2～10, time 5～30min/ time, number of times 3～10 times.
4, the method for a kind of producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed according to claim 1, it is characterized in that: described raw material is all rapeseed peeling cold pressed cakes, has both comprised general Semen Brassicae campestris, has also comprised two low (low erucic acid, low sulfatide) rapeseed peeling cold pressed cakes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100183906A CN100357315C (en) | 2005-03-17 | 2005-03-17 | Method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100183906A CN100357315C (en) | 2005-03-17 | 2005-03-17 | Method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed |
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| Publication Number | Publication Date |
|---|---|
| CN1683390A true CN1683390A (en) | 2005-10-19 |
| CN100357315C CN100357315C (en) | 2007-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2005100183906A Expired - Fee Related CN100357315C (en) | 2005-03-17 | 2005-03-17 | Method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020532983A (en) * | 2017-09-11 | 2020-11-19 | フラウンホッファー−ゲゼルシャフト ツァ フェルダールング デァ アンゲヴァンテン フォアシュンク エー．ファオ | Methods for Obtaining Protein Preparations from Sunflower and / or Oilseed Oil Seeds and Protein Preparations |
| CN115336664A (en) * | 2022-08-26 | 2022-11-15 | 鼎新思远重庆粮油科技有限公司 | Method for producing rapeseed tissue protein by deoiling rapeseed kernel by using new water agent method |
-
2005
- 2005-03-17 CN CNB2005100183906A patent/CN100357315C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020532983A (en) * | 2017-09-11 | 2020-11-19 | フラウンホッファー−ゲゼルシャフト ツァ フェルダールング デァ アンゲヴァンテン フォアシュンク エー．ファオ | Methods for Obtaining Protein Preparations from Sunflower and / or Oilseed Oil Seeds and Protein Preparations |
| JP7261225B2 (en) | 2017-09-11 | 2023-04-19 | フラウンホッファー－ゲゼルシャフト ツァ フェルダールング デァ アンゲヴァンテン フォアシュンク エー．ファオ | Method and protein preparation for obtaining protein preparations from sunflower and/or rapeseed oilseeds |
| CN115336664A (en) * | 2022-08-26 | 2022-11-15 | 鼎新思远重庆粮油科技有限公司 | Method for producing rapeseed tissue protein by deoiling rapeseed kernel by using new water agent method |
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| Publication number | Publication date |
|---|---|
| CN100357315C (en) | 2007-12-26 |
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Assignee: Fujian Nanping Days Machinery Manufacturing Co. Assignor: Wuhan Polytechnic University Contract fulfillment period: 2009.7.5 to 2025.3.16 contract change Contract record no.: 2009350000178 Denomination of invention: Method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed Granted publication date: 20071226 License type: Exclusive license Record date: 2009.8.18 |
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Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2009.7.5 TO 2025.3.16; CHANGE OF CONTRACT Name of requester: FUJIAN NANPING TIANHE MACHINERY MANUFACTURING CO., Effective date: 20090818 |
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Granted publication date: 20071226 |