CN115336664A - Method for producing rapeseed tissue protein by deoiling rapeseed kernel by using new water agent method - Google Patents
Method for producing rapeseed tissue protein by deoiling rapeseed kernel by using new water agent method Download PDFInfo
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- CN115336664A CN115336664A CN202211032762.0A CN202211032762A CN115336664A CN 115336664 A CN115336664 A CN 115336664A CN 202211032762 A CN202211032762 A CN 202211032762A CN 115336664 A CN115336664 A CN 115336664A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/22—Working-up of proteins for foodstuffs by texturising
- A23J3/26—Working-up of proteins for foodstuffs by texturising using extrusion or expansion
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Abstract
The invention specifically discloses a method for producing rapeseed histon by deoiling rapeseed kernels by using a new water agent method, which comprises the following steps: step 1: placing the deoiled rapeseed kernel by a new water aqua method into a leaching device, and continuously extracting for 1-24 hours by using 1-10 times of ethanol water solution or until the content of the solid glucosinolate is lower than 120mg/kg on a dry basis; step 2: completely removing the ethanol water solution to obtain a solid; and step 3: crushing the obtained solid and sieving to obtain rape seed kernel removed powder; and 4, step 4: adding 0-10wt% of starch into the obtained deoiled rapeseed kernel powder, adding water for blending, and adjusting the water content to 30-70wt%; and 5: fully and uniformly stirring the mixture, and then texturizing the mixture by an extrusion bulking machine to obtain the rapeseed histoprotein. The rapeseed histon prepared by the method has the advantages of high water absorption, high elasticity and filamentous structure, and the preparation method is simple.
Description
Technical Field
The invention belongs to the technical field of agricultural and sideline product processing, and particularly relates to a method for producing rapeseed tissue protein by deoiling rapeseed kernels by using a new aqueous solution method.
Background
Cardiovascular and cerebrovascular diseases (or hypertension, hyperglycemia, and hyperlipidemia) are mainly caused by improper diet, and the development of the diseases starts from childhood. There are 2 billion overweight or obese people, 2.7 billion hypertensive patients, 1.6 billion dyslipidemic people (including 1 billion hyperlipidemic people); in addition, nearly half of people in China have abnormal blood sugar (the risk of diabetes is increased or part of people already suffer from diabetes, and the health of heart and cerebral vessels is damaged). Therefore, from childhood, adherence to a healthy diet is essential to reduce the risk of these patients as described above.
OECD/FAO reports that food protein is consumed at 31 kg throughout the world today in the year, and will further increase to 41 kg by 2029. However, the traditional protein resource, animal meat, contains large amounts of cholesterol and saturated fat, and long-term consumption of such food in large quantities increases the risk of cardiovascular and cerebrovascular diseases. These are one of the most important forces driving vegetable protein meat to replace animal protein.
In addition, the international cancer research institute in WHO has administered classification of processed meats (e.g., hot dogs, ham, bacon, and sausage) into a first class of carcinogens and classification of fresh red meat into a second class of carcinogens. According to the Beyond Meat official network, long-term consumption of regular Meat increases the risk of 16% of cancer and 21% of heart disease. Meanwhile, processed red meat (such as sausage or minced beef) contains nitrate and nitrite, which are harmful to human health. Animals and livestock are susceptible to various diseases, such as mad cow disease, swine flu, foot and mouth disease, avian flu, etc., which may pose health risks. Drugs or antibiotics are required in the production of animal protein foods, and have a residual and adverse effect on the human body. These are also one of the most important forces driving vegetable protein meat to replace animal protein.
The annual output of rapeseeds in 2021 years in China is as high as 1400 million tons, and the rapeseeds belong to the 1 st oil material. Therefore, the resource amount is abundant. Rapeseed kernel is rich in protein (above about 25-30%), and its amino acid balance (especially lysine content) is superior to almost all oilseed proteins (including soy protein); the titer is as high as 3.0-3.5, which is superior to casein in milk (2.5). The concentrated rapeseed protein is added to the beef emulsion, so that meat quality and fat can be absorbed in the cooking process, and the flavor can be enhanced. These indicate that rapeseed kernel is an excellent protein resource.
At present, edible oil is processed by basically utilizing rapeseeds in China; the remaining protein-rich defatted material is only used as cheap feed and fertilizer, and cannot be applied to the food industry. Therefore, a great deal of waste is caused, and economic and social benefits are low. The invention provides a method for producing rapeseed histoprotein by using a new water agent method to remove rapeseed kernels, which fills the blank in the market and the technology in this aspect.
Disclosure of Invention
The invention aims to provide a method for producing rapeseed tissue protein by using a new water agent method to remove rapeseed kernels, which has reasonable and ingenious design, high utilization rate of raw materials, environmental protection and capability of furthest exerting the nutritional and functional advantages of the rapeseed protein and filling the blank in the production technology of edible rapeseed tissue protein at home and abroad.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a method for producing rapeseed histoprotein by deoiling rapeseed kernels by using a new water agent method comprises the following steps:
step 1: placing the deoiled semen Brassicae campestris kernel in a leaching device, continuously extracting with 1-10 times of ethanol water solution for 1-24 hr or until the content of solid glucosinolate is less than 120mg/kg (0.3 mmol/kg) on dry basis;
step 2: completely removing the ethanol water solution to obtain a solid;
and step 3: crushing the obtained solid and sieving to obtain rape seed kernel removed powder;
and 4, step 4: adding 0-10wt% of starch into the obtained deoiled rapeseed kernel powder, adding water for blending, and adjusting the water content to 30-70wt%;
and 5: fully and uniformly stirring the mixture, and then texturizing the mixture by an extrusion bulking machine to obtain the rapeseed histoprotein.
Further, the concentration of the ethanol aqueous solution is 20-100% (v/v).
Further, the screen is a 80-120 mesh screen.
Further, the extrusion-puffing machine is a single-screw extrusion-puffing machine or a double-screw extrusion-puffing machine.
Further, the leacher is in the form of a flat rotating type or a ring type or a tank group.
The invention has the beneficial effects that: the rapeseed histoprotein prepared by the method has the advantages of high water absorption, high elasticity and filamentous structure, and the preparation method is simple, improves the added value of the rape seed kernel, and has obvious economic and social benefits.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The new aqueous agent method deoiled rapeseed kernel raw material adopted by the invention is the deoiled rapeseed kernel produced by the method for extracting the plant seed oil by water disclosed in the earlier patent application with the application number of CN201910956760.2, and the deoiled rapeseed kernel raw material is directly adopted without being dried. The oil and fat content of this material is sufficiently low to a level that does not interfere with tissue protein production, and the protein content is low in protein deformation.
The judgment of the content level of safe eating of the invention refers to the standard proposed by the European food safety administration for approving rapeseed meal as a new food or an additive thereof, and the specific conditions are as follows: glucosinolates <0.3mmol/kg (< 120 mg/kg), phytic acid <1.5% and proanthocyanidins <3,000mg/kg (EFSA Journal 2020 (7): 6197).
Example 1
A method for producing rapeseed histoprotein by deoiling rapeseed kernels by using a new water agent method comprises the following operation steps: putting the deoiled rapeseed kernels by the new aqueous solution method into a flat-bed type leacher, continuously extracting for 1 hour by using 20% (v/v) ethanol aqueous solution of 1 time, completely removing the ethanol aqueous solution to obtain solid, crushing the obtained solid, sieving the crushed solid by using a 80-mesh sieve to obtain deoiled rapeseed kernel powder, adding 10wt% of starch into the obtained deoiled rapeseed kernel powder, adding water for modulation, adjusting the water content to 30wt%, fully and uniformly stirring, and then organizing by using a double-screw extrusion bulking machine to obtain the rapeseed tissue protein product.
Example 2
A method for producing rapeseed histoprotein by deoiling rapeseed kernels by using a new water agent method comprises the following operation steps: putting the rape seed kernels removed by the new aqueous method into an annular leacher, continuously extracting with 6 times of 50% (v/v) ethanol aqueous solution until the content of the glucosinolate in the solid is lower than 120mg/kg (0.3 mmol/kg) on a dry basis, completely removing the ethanol aqueous solution to obtain a solid, crushing the obtained solid, sieving the crushed solid with a 90-mesh sieve to obtain rape seed kernel powder, adding 9wt% of starch into the obtained rape seed kernel powder, adding water for modulation, regulating the water content to 60wt%, fully and uniformly stirring, and then organizing by a single-screw extrusion bulking machine to obtain the rape seed tissue protein product.
Example 3
A method for producing rapeseed tissue protein by using a new water agent method to remove rapeseed kernels comprises the following operation steps: putting the deoiled rapeseed kernels by the new aqueous method into a ring-shaped leacher, continuously extracting for 24 hours by using 10 times of 100% (v/v) ethanol, completely removing the ethanol to obtain a solid, crushing the obtained solid, sieving the crushed solid by using a 120-mesh sieve to obtain deoiled rapeseed kernel powder, adding 9wt% of starch into the obtained deoiled rapeseed kernel powder, adding water for modulation, regulating the water content to 70wt%, fully and uniformly stirring, and then texturizing by using a double-screw extrusion bulking machine to obtain the rapeseed tissue protein product.
The invention is further illustrated below with reference to test examples:
the results of examining the contents of glucosinolate, phytic acid and proanthocyanidin in the rapeseed histones obtained in each example are shown in Table 1, and it can be seen from Table 1 that the rapeseed histones produced by the method of the present invention meet the standards approved by the European food safety agency.
TABLE 1 content of key safety factor index of rapeseed histon obtained in each example
| Examples | Sulfuric glucoside | Phytic acid | Proanthocyanidins |
| Example 1 | 116mg/kg | 1.4％ | 2,400mg/kg |
| Example 2 | 115mg/kg | 1.3％ | 2,300mg/kg |
| Example 3 | 118mg/kg | 1.3％ | 2,350mg/kg |
The results of examining the key physical properties of the rapeseed histones obtained in the examples are shown in Table 2, and it can be seen from Table 2 that the rapeseed histones produced by the method of the present invention have high water absorbability, high elasticity and a filamentous structure.
TABLE 2 Key physical Properties of rapeseed histones obtained in the examples
| Examples | Water absorption | Elasticity | Fibril-like structure |
| Example 1 | 3.8 times of | Has good elasticity when chewed | Good filaments visible to the naked eye |
| Example 2 | 3.9 times of | Has good elasticity when chewed | Good filaments visible to the naked eye |
| Example 3 | 3.8 times of | Has good elasticity when chewed | Good filaments visible to the naked eye |
Through repeated tests, completely removing the ethanol water solution to obtain a solid, crushing the obtained solid, sieving the crushed solid with a 80-120-mesh sieve to obtain rape seed kernel removed powder, adding 0-10wt% of starch into the obtained rape seed kernel removed powder, adding water for blending, and adjusting the water content to 30-70wt%; the rapeseed histones obtained by texturizing through the extrusion bulking machine after fully and uniformly stirring has the advantages of high water absorption, high elasticity and filamentous structure of thin fibers.
Moreover, after repeated tests, the glucosinolate, the phytic acid and the proanthocyanidin of the prepared rapeseed histon can reach the standards approved by the European food safety bureau under the same other conditions by continuously extracting 1-10 times of 20-100% (v/v) ethanol aqueous solution for 1-24 hours.
It should be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The foregoing are embodiments of the present invention and are not intended to limit the scope of the invention to the particular forms set forth in the specification, which are set forth in the claims below, but rather are to be construed as the full breadth and scope of the claims, as defined by the appended claims, as defined in the appended claims, in order to provide a thorough understanding of the present invention. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be defined by the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (5)
1. A method for producing rapeseed tissue protein by using a new water agent method to remove rapeseed kernels is characterized in that: the method comprises the following steps:
step 1: placing the deoiled rapeseed kernel by a new water aqua method into a leaching device, and continuously extracting for 1-24 hours by using 1-10 times of ethanol water solution or until the content of the solid glucosinolate is lower than 120mg/kg on a dry basis;
step 2: completely removing the ethanol water solution to obtain a solid;
and step 3: crushing the obtained solid and sieving to obtain rape seed kernel removed powder;
and 4, step 4: adding 0-10wt% of starch into the obtained deoiled rapeseed kernel powder, adding water for blending, and adjusting the water content to 30-70wt%;
and 5: fully and uniformly stirring the mixture, and then texturizing the mixture by an extrusion bulking machine to obtain the rapeseed histoprotein.
2. The method for producing rapeseed histon by deoiling rapeseed kernel according to claim 1 by using a new aqueous solution method, characterized in that: the concentration of the ethanol water solution is 20-100% (v/v).
3. The method for producing rapeseed histon by deoiling rapeseed kernel according to claim 1 by using a new aqueous solution method, characterized in that: the screen is a 80-120 mesh screen.
4. The method for producing rapeseed tissue protein by de-oiling rapeseed kernels by a new aqueous method according to claim 1, wherein the new aqueous method comprises the following steps: the extrusion bulking machine is a single-screw extrusion bulking machine or a double-screw extrusion bulking machine.
5. The method for producing rapeseed histon by deoiling rapeseed kernel according to claim 1 by using a new aqueous solution method, characterized in that: the leacher is in the form of a flat rotating type or a ring type or a tank group.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211032762.0A CN115336664A (en) | 2022-08-26 | 2022-08-26 | Method for producing rapeseed tissue protein by deoiling rapeseed kernel by using new water agent method |
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| CN202211032762.0A CN115336664A (en) | 2022-08-26 | 2022-08-26 | Method for producing rapeseed tissue protein by deoiling rapeseed kernel by using new water agent method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1683390A (en) * | 2005-03-17 | 2005-10-19 | 武汉工业学院 | Method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed |
| CN1837234A (en) * | 2005-03-25 | 2006-09-27 | 武汉工业学院 | Process for preparing low phytic acid rapeseed protein concentrate by using water and ethanol |
| CN101278700A (en) * | 2008-05-15 | 2008-10-08 | 国家粮食储备局西安油脂科学研究设计院 | Alcohol method for continuously extracting vegetable seed concentrating protein and simultaneously removing toxicity and anti-nutrition component in rapeseed dregs |
| CN104322858A (en) * | 2014-10-20 | 2015-02-04 | 合肥工业大学 | Method for preparing textured wheat protein by adopting twin-screw extrusion |
| CN105685369A (en) * | 2016-01-27 | 2016-06-22 | 山东三维大豆蛋白有限公司 | Method for producing histones by utilizing soybean protein concentrate |
| CN107549444A (en) * | 2017-09-28 | 2018-01-09 | 福建安井食品股份有限公司 | A kind of preparation method of textured soybean protein |
-
2022
- 2022-08-26 CN CN202211032762.0A patent/CN115336664A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1683390A (en) * | 2005-03-17 | 2005-10-19 | 武汉工业学院 | Method for producing poisonless rape seed concentrate protein from coat removed cold pressed cake of rapeseed |
| CN1837234A (en) * | 2005-03-25 | 2006-09-27 | 武汉工业学院 | Process for preparing low phytic acid rapeseed protein concentrate by using water and ethanol |
| CN101278700A (en) * | 2008-05-15 | 2008-10-08 | 国家粮食储备局西安油脂科学研究设计院 | Alcohol method for continuously extracting vegetable seed concentrating protein and simultaneously removing toxicity and anti-nutrition component in rapeseed dregs |
| CN104322858A (en) * | 2014-10-20 | 2015-02-04 | 合肥工业大学 | Method for preparing textured wheat protein by adopting twin-screw extrusion |
| CN105685369A (en) * | 2016-01-27 | 2016-06-22 | 山东三维大豆蛋白有限公司 | Method for producing histones by utilizing soybean protein concentrate |
| CN107549444A (en) * | 2017-09-28 | 2018-01-09 | 福建安井食品股份有限公司 | A kind of preparation method of textured soybean protein |
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