CN1650823A - Artificial hair mammilla substition structure and its manufacturing method - Google Patents
Artificial hair mammilla substition structure and its manufacturing method Download PDFInfo
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- CN1650823A CN1650823A CN 200410091871 CN200410091871A CN1650823A CN 1650823 A CN1650823 A CN 1650823A CN 200410091871 CN200410091871 CN 200410091871 CN 200410091871 A CN200410091871 A CN 200410091871A CN 1650823 A CN1650823 A CN 1650823A
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Abstract
An artificial hair papilla for treating the hypotrichosis and alopecia is composed of the hair papilla cells and a three-layer hollow microbag consisting of sodium alginate, polylsine and sodium alginate layers for containing said hair papilla cells in it.
Description
Technical field
The invention belongs to alternative structure of a kind of human body or animal tissue and preparation method thereof, be specifically related to hair mammilla substition structure of a kind of manual manufacture and preparation method thereof.
Background technology
The hair papilla of human body or animal is positioned at the bottom of hair follicle, comprises hair papilla cell 2 and basement membrane 3 (see figure 1)s.Research and experiment confirm, hair papilla all plays important central role for inducing hair follicle regeneration and keeping hair growth, and one of them important mechanisms is hair papilla constantly secretion and transmit relevant signal factor to hair follicle in this process.If but after separately hair papilla cell being transplanted to skin, it induces hair follicle regeneration and keeps the effect of hair growth but very unstable, this reason is very complicated, wherein important factor is, the basement membrane of hair papilla can provide spatial growing space for parcel hair papilla cell wherein, allow hair papilla cell present the concentration growth, and characteristic and its function of concentration growth are closely related.If merely hair papilla cell is carried out the transplanting from body or allosome, hair papilla cell does not just have above-mentioned growth conditions, thereby is difficult to play the effect same with hair papilla.Because hair papilla cell is induced hair follicle regeneration and kept the effect instability of hair growth, thereby can't apply to and treat various hairs disappearance property diseases clinically.
Hair papilla can carry out In vitro culture in culture dish, but, the result of hair papilla In vitro culture just makes the quantity amplification of hair papilla cell, rather than makes the quantity amplification of hair papilla, promptly can not be obtained the hair papilla of greater number by the hair papilla of a multiple amount in external direct cultivation.In other words, in the prior art, people have the hair papilla of inducing the hair follicle regeneration function in order to obtain, its unique channel can only be directly from separating from the skin of body or allosome, but the graft application of allosome or xenogenesis hair papilla easily produces the repulsion immunoreation, and can be very limited from body number of people fur nipple source for what transplant.Therefore, though having understood hair papilla deeply, people have the characteristic of inducing hair follicle regeneration and keeping hair growth, but because the source of hair papilla is subjected to very big restriction, people fail various hair disappearance property diseases are treated in the characteristic extensive use of hair papilla clinically too now, and hair disappearance property treatment of diseases remains global problem.
On the other hand, the film formed hollow microcapsule of sodium alginate-poly-D-lysine-sodium alginate three-decker (common name " APA microcapsule ") is in the extensive use of the field of cell transplantation, this three-decker film is a semipermeable membrane, only allow molecular weight to free in and out less than about 100,000 small-molecule substance, thereby the APA microcapsule structure can allow on the one hand nutrient substance, cytokine, somatomedin and relevant signal factor freely pass through, can effectively completely cut off the attack of macromolecular complex confrontation capsule inner cells such as IgG antibody on the other hand again, the function of immune isolation of APA microcapsule gains public acceptance.But, up to now, people have just noticed its function of immune isolation for the understanding and the utilization of APA microcapsule performance and effect, and do not find that as yet the APA microcapsule has other performance and effect, still find no the report that is used for artificial hair mammilla substition structure about the APA microcapsule both at home and abroad.
Summary of the invention
The objective of the invention is to provide a kind of artificial hair mammilla substition structure, and it has the function of inducing hair follicle regeneration and keeping hair growth, and this structure can obtain by manual manufacture external, and it is extensive that it makes material source, and quantity is unrestricted.The present invention also aims to provide the manufacture method of this artificial hair mammilla substition structure.
For realizing above purpose, artificial hair mammilla substition structure of the present invention comprises hair papilla cell, it is characterized in that: the film formed hollow microcapsule of sodium alginate-poly-D-lysine-sodium alginate three-decker is arranged in the hair papilla cell coated outside, the diameter of microcapsule is 200～650 μ m, and the wall thickness of microcapsule is 2～12 μ m.
Be preferably, the diameter of microcapsule is 300～500 μ m in the above-mentioned artificial hair mammilla substition structure, and the wall thickness of microcapsule is 4～8 μ m.Like this, the form of microcapsule is similar substantially to the form of number of people fur nipple basement membrane.
Be more preferably the hair papilla cell behaviour scalp hair papilla cell in the microcapsule.
Hair papilla cell in the above-mentioned microcapsule also can be the hair papilla cell of animal.
The manufacture method of above-mentioned artificial hair mammilla substition structure comprises following key step successively: 1., hair papilla cell is suspended from the sodium alginate soln that concentration is 15～25g/L, form suspension, make in every milliliter of suspension to contain 1.5～9 * 10
5Individual hair papilla cell; 2., utilizing the high voltage electric field generator for microcapsules to make suspension that 1. step obtain form diameter is the micro-droplet status of 200～650 μ m, splashes into the CaCl that concentration is 9～14g/L then
2In the solution, obtain solid microsphere shape precipitate; 3., solid microsphere shape precipitate that 2. step is obtained joins in the Poly-L-Lysine Solution that concentration is 0.5～1.5g/L, acts on 8～20 minutes, obtains solid microsphere shape precipitate; 4., solid microsphere shape precipitate that 3. step is obtained joins in the sodium alginate soln that concentration is 1.5～2.5g/L, acts on 3～10 minutes, obtains solid microsphere shape precipitate; 5., solid microsphere shape precipitate that 4. step is obtained joins in the liquor sodii citratis that concentration is 0.03～0.07mol/L, acts on 5～20 minutes, obtains the microspheroidal precipitate of hollow structure; 6., hollow structure microspheroidal precipitate that 5. step is obtained cleans with normal saline, can obtain artificial hair mammilla substition structure.The artificial hair mammilla substition structure that obtains can change in the DMEM liquid that contains hyclone to be cultivated, and preserves.
Be preferably, above-mentioned steps 1. among, the concentration that makes hair papilla cell in the suspension is every milliliter 4～6 * 10
5Individual.
Be more preferably, in the above-mentioned manufacture method, above-mentioned steps 1. before, earlier place vessel to cultivate hair papilla, with the hair papilla cell of moving out in the hair papilla go down to posterity cultivate after, obtain the hair papilla cell that quantity increases at double, and then carry out above-mentioned steps 1..
In the above-mentioned manufacture method, the hair papilla cell of employing can be people's scalp hair papilla cell.
In the above-mentioned manufacture method, the hair papilla cell of employing also can be the hair papilla cell of animal.
In above-mentioned each artificial hair mammilla substition structure, the quantity of hair papilla cell can be 10～100, is preferably 15～40.
The present invention has the following advantages and effect:
1. the basement membrane of shape, diameter and the organism hair papilla of the sodium alginate-poly-D-lysine among the present invention-sodium alginate three-decker film (APA microcapsule) is roughly similar, it all is hollow class spheroidal structure, both wall thickness difference are little, uniform honeycomb-like pore structure is all arranged on the wall, and pore size is similar, all be semipermeable membrane character, both are closely similar aspect structure, form.
2, the APA microcapsule among the present invention not only has the two big common functions (can allow small-molecule substance freely pass through and the attack of isolated macromolecular substances) of traditional APA microcapsule, but also has the function of the basement membrane of organism hair papilla, can think that parcel hair papilla cell wherein provides enough three-dimensional growth spaces, make hair papilla cell be able to grow in the mode of aggregation, and help allowing hair papilla cell paste the growth of APA microcapsule wall, cater to the demand of hair papilla cell physiological characteristics.Therefore, artificial hair mammilla substition structure of the present invention is transplanted on one's body human body or the animal, can be played the effect of inducing hair follicle regeneration and keeping hair growth equally more stablely.
3, artificial hair mammilla substition structure of the present invention both can carry out autotransplantation (promptly adopt from go down to posterity hair papilla cell that amplification cultivation comes out of body and make the transplanting that the hair mammilla substition structure that obtains carries out), also can carry out allograft, also can carry out xenotransplantation, have broad prospect of application.When carrying out autotransplantation, the main effect of APA microcapsule is not known function of immune isolation among the present invention, but plays the function that substitutes basement membrane, and this performance of APA microcapsule is strange, therefore demonstrates fully creativeness of the present invention.When carrying out allograft or xenotransplantation, the main effect of APA microcapsule has two aspects among the present invention, the first plays the function that substitutes basement membrane, it two is to play known immune buffer action to a certain extent, because the APA microcapsule can be realized dual-use material in this case, and one of them effect is strange, embodies creativeness of the present invention more fully.
4, artificial hair mammilla substition structure of the present invention plays the effect identical with hair papilla, and can be in biological external structure, the hair papilla insufficient problem of originating is readily solved, it both can utilize hair papilla cell from body to go down to posterity in advance and make after the amplification cultivation, can utilize allochthonous hair papilla cell or xenogeneic hair papilla cell to make again, particularly can adopt the alternative structure of animal wool papillose cell producer scalp hair papilla, so its raw material sources, output is unrestricted, make the biological characteristics that imitates hair papilla clinically treat the possibility of various hairs disappearance property diseases on becoming a reality, have broad prospect of application.
The experiment effect of artificial hair mammilla substition structure of the present invention aspect morphosis observation and transplanting is remarkable.
Utilize method of the present invention to make and obtain artificial hair mammilla substition structure, and carry out with the number of people fur nipple of fresh separated that general form, Ultrastructural microscopically are observed, contrast, the result is as follows: the basement membrane of the number of people fur nipple of fresh separated is a spheroidal, diameter 300～450 μ m, basement membrane is very smooth, thick about 5～6 μ m are uniform alveolate texture.And the artificial hair mammilla substition structure that experiment obtains is spheroidal equally, diameter 300～500 μ m, size evenly, smooth surface, about 4～8 μ m of cyst membrane wall thickness, be uniform alveolate texture, and the cell no significant difference in hair papilla cell wherein and the fresh separated hair papilla, the hair papilla of visible human hair papilla alternative structure and fresh separated is closely similar aspect configuration.
The inventive method is made the 1 milliliter of number of people fur nipple alternative structure (containing more than 1000 approximately) that obtains, it is subcutaneous to be injected in the nude mouse back with syringe needle, observe the situation that it induces local hair follicle structure to form, the result is as follows: transplant the 4th week of back, in subcutaneous tissue of back, as seen the complete hair follicle structure of a large amount of growths forms, the HE demonstration of dyeing, hair follicle is the concentric circular arrangement by several layers of epithelial cell and forms, the center is the keratinization material of eosinophilic staining, simultaneously, grow at the visible hair of nude mouse skin of back.And in away from the nude mice skin of transplanting the zone, the hair follicle structure of accidental atrophy only.As seen the APA microcapsule of artificial hair mammilla substition structure can be brought into play the effect identical with basement membrane in the hair papilla, and artificial hair mammilla substition structure has the function of inducing hair follicle regeneration and keeping hair growth equally.
The inventive method is made the 1 milliliter of number of people fur nipple alternative structure (containing more than 1000 approximately) that obtains, be injected in people's scalp alopecia position with syringe needle, observe the situation that it induces local hair follicle structure to form, the result is as follows: transplant the 4th week of back, visible a large amount of complete hair follicle structure formation of growing at people's scalp alopecia position, transplant the 6th week of back, visible transplanting place scalp grows hair.Therefore, artificial number of people fur nipple alternative structure has the function of inducing hair follicle regeneration and keeping hair growth, can effectively treat alopecia.
Description of drawings
Fig. 1 is the structural representation of human body or animal wool nipple.
Fig. 2 is the structural representation of inventor workman's scalp hair mammilla substition structure.
The specific embodiment
Embodiment one
See Fig. 2, the artificial hair mammilla substition structure of this embodiment comprises hollow microcapsule 1, this hollow microcapsule is sodium alginate-poly-D-lysine-three layers of semipermeable membrane structure of sodium alginate, the diameter of microcapsule is 300 μ m, the wall thickness of microcapsule is 4 μ m, begin to be enclosed with 15 people's scalp hair papilla cells 2 in the microcapsule, certainly, the quantity of later cell can be grown gradually, be increased.
Embodiment two
The artificial hair mammilla substition structure of this embodiment comprises the film building hollow microcapsule of sodium alginate-poly-D-lysine-sodium alginate three-layered node, the diameter of microcapsule is 500 μ m, the wall thickness of microcapsule is 8 μ m, is enclosed with 40 people's scalp hair papilla cells in the microcapsule, and its structure and embodiment one are basic identical.
Embodiment three
The artificial hair mammilla substition structure of this embodiment comprises the film building hollow microcapsule of sodium alginate-poly-D-lysine-sodium alginate three-layered node, the diameter of microcapsule is 400 μ m, the wall thickness of microcapsule is 6 μ m, is enclosed with 25 people's scalp hair papilla cells in the microcapsule, and its structure and embodiment one are basic identical.
Embodiment four
The artificial hair mammilla substition structure of this embodiment comprises the film building hollow microcapsule of sodium alginate-poly-D-lysine-sodium alginate three-layered node, the diameter of microcapsule is 500 μ m, the wall thickness of microcapsule is 6 μ m, is enclosed with the hair papilla cell of 45 sheep in the microcapsule, and its structure and embodiment one are basic identical.
Embodiment five
The artificial hair mammilla substition structure of this embodiment comprises the film building hollow microcapsule of sodium alginate-poly-D-lysine-sodium alginate three-layered node, the diameter of microcapsule is 220 μ m, the wall thickness of microcapsule is 2 μ m, be enclosed with the hair papilla cell of 80 white mice in the microcapsule, its structure and embodiment one are basic identical.
Embodiment six
The artificial hair mammilla substition structure of this embodiment comprises the film building hollow microcapsule of sodium alginate-poly-D-lysine-sodium alginate three-layered node, the diameter of microcapsule is 650 μ m, the wall thickness of microcapsule is 11 μ m, is enclosed with the hair papilla cell of 40 red deer in the microcapsule, and its structure and embodiment one are basic identical.
Embodiment seven
The manufacture method of artificial hair mammilla substition structure, comprise following key step successively: place vessel to cultivate number of people fur nipple 1., earlier, the hair papilla cell of moving out in the hair papilla is gone down to posterity and obtain the hair papilla cell that quantity increases at double, hair papilla cell is suspended from the sodium alginate soln that concentration is 20g/L, forms every milliliter and contain 5 * 10
5The suspension of individual hair papilla cell; 2., utilizing the high voltage electric field generator for microcapsules to make suspension that 1. step obtain form diameter is the micro-droplet status of 450 μ m, splashes into the CaCl that concentration is 11g/L then
2In the solution, treat complete post precipitation, remove supernatant, obtain solid microsphere shape precipitate, normal saline cleans 3 times; 3., solid microsphere shape precipitate that 2. step is obtained joins in the Poly-L-Lysine Solution that concentration is 1g/L, acts on 10 minutes, obtains solid microsphere shape precipitate, normal saline cleans 3 times; 4., solid microsphere shape precipitate that 3. step is obtained joins in the sodium alginate soln that concentration is 2g/L, acts on 4 minutes, obtains solid microsphere shape precipitate, normal saline cleans 3 times, each 3 minutes; 5., solid microsphere shape precipitate that 4. step is obtained joins in the liquor sodii citratis that concentration is 0.05mol/L, acts on 6 minutes, obtains the microspheroidal thing of hollow structure; 6., the microspheroidal thing of hollow structure that 5. step is obtained cleans 3 times with normal saline, can obtain artificial hair mammilla substition structure, this artificial hair mammilla substition structure is transferred in the DMEM liquid that contains 10% hyclone cultivates then, preserve.
Above-mentioned steps " being suspended from " in 1. is meant hair papilla cell is evenly spread in the sodium alginate soln.
Embodiment eight
The manufacture method of artificial hair mammilla substition structure, comprise following key step successively: place vessel to cultivate number of people fur nipple 1., earlier, go down to posterity after the hair papilla cell of moving out in the hair papilla is merged and obtain the hair papilla cell of quantity amplification, hair papilla cell is suspended from the sodium alginate soln that concentration is 15g/L then, forms every milliliter and contain 1.5 * 10
5The suspension of individual hair papilla cell; 2., utilizing the high voltage electric field generator for microcapsules to make suspension that 1. step obtain form diameter is the micro-droplet status of 400 μ m, splashes into the CaCl that concentration is 9g/L then
2In the solution, treat complete post precipitation, remove supernatant, obtain solid microsphere shape precipitate, normal saline cleans 3 times; 3., solid microsphere shape precipitate that 2. step is obtained joins in the Poly-L-Lysine Solution that concentration is 0.5g/L, acts on 20 minutes, obtains solid microsphere shape precipitate, normal saline cleans 3 times; 4., solid microsphere shape precipitate that 3. step is obtained joins in the sodium alginate soln that concentration is 1.5g/L, acts on 10 minutes, obtains solid microsphere shape precipitate, normal saline cleans 3 times; 5., solid microsphere shape precipitate that 4. step is obtained joins in the liquor sodii citratis that concentration is 0.03mol/L, acts on 20 minutes, obtains the microspheroidal thing of hollow structure; 6., the microspheroidal thing of hollow structure that 5. step is obtained cleans 3 times with normal saline, each 3 minutes, can obtain artificial hair mammilla substition structure, this artificial hair mammilla substition structure is changed in the DMEM liquid that contains 10% hyclone cultivate then, preserve.
Embodiment nine
1., be that the sodium alginate soln of 25g/L mixes with number of people fur papillose cell and concentration the manufacture method of artificial hair mammilla substition structure comprises following key step successively:, form every milliliter and contain 9 * 10
5The suspension of individual hair papilla cell; 2., utilizing the high voltage electric field generator for microcapsules to make suspension that 1. step obtain form diameter is the micro-droplet status of 500 μ m, splashes into the CaCl that concentration is 14g/L then
2In the solution, treat complete post precipitation, remove supernatant, obtain solid microsphere shape precipitate, normal saline cleans 3 times; 3., solid microsphere shape precipitate that 2. step is obtained joins in the Poly-L-Lysine Solution that concentration is 2.5g/L, acts on 8 minutes, obtains solid microsphere shape precipitate, normal saline cleans 3 times; 4., solid microsphere shape precipitate that 3. step is obtained joins in the sodium alginate soln that concentration is 2.5g/L, acts on 3 minutes, obtains solid microsphere shape precipitate, normal saline cleans 3 times; 5., solid microsphere shape precipitate that 4. step is obtained joins in the liquor sodii citratis that concentration is 0.07mol/L, acts on 5 minutes, obtains the microspheroidal thing of hollow structure; 6., the microspheroidal thing of hollow structure that 5. step is obtained cleans 3 times with normal saline, can obtain artificial hair mammilla substition structure, this artificial hair mammilla substition structure changed in the DMEM liquid that contains 10% hyclone cultivate then, preserve.
Embodiment ten
The step of this method 1., be that the sodium alginate soln of 20g/L mixes with sheep's wool papillose cell and concentration, form every milliliter and contain 5 * 10
5The suspension of individual sheep's wool papillose cell; The step of this method is 2.～6. identical with embodiment seven.
Embodiment 11
The step of this method 1., be that the sodium alginate soln of 20g/L mixes with white mice hair papilla cell and concentration, form every milliliter and contain 4 * 10
5The suspension of individual white mice hair papilla cell; 2., utilizing the high voltage electric field generator for microcapsules to make suspension that 1. step obtain form diameter is the micro-droplet status of 200 μ m, splashes into the CaCl that concentration is 11g/L then
2In the solution, treat complete post precipitation, remove supernatant, obtain the microspheroidal precipitate, normal saline cleans 3 times; Following steps are 3.～6. identical with embodiment eight.
Embodiment 12
The step of this method 1., be that the sodium alginate soln of 20g/L mixes with red deer (red deer) hair papilla cell and concentration, form every milliliter and contain 6 * 10
5The suspension of individual red deer hair papilla cell; 2., utilizing the high voltage electric field generator for microcapsules to make suspension that 1. step obtain form diameter is the micro-droplet status of 650 μ m, splashes into the CaCl that concentration is 11g/L then
2In the solution, treat complete post precipitation, remove supernatant, obtain solid microsphere shape precipitate, normal saline cleans 3 times; Following steps are 3.～6. identical with embodiment nine.
In the foregoing description, the product that the high voltage electric field generator for microcapsules can adopt Shanghai University of Science and Technology to produce, its method of regulating control diameter of droplets size belongs to known technology.
Claims (9)
1, a kind of artificial hair mammilla substition structure, comprise hair papilla cell, it is characterized in that: in the hair papilla cell coated outside the film building hollow microcapsule of sodium alginate-poly-D-lysine-sodium alginate three-layered node is arranged, the diameter of microcapsule is 200～650 μ m, and the wall thickness of microcapsule is 2～12 μ m.
2, according to the artificial hair mammilla substition structure of claim 1, it is characterized in that: the diameter of microcapsule is 300～500 μ m, and the wall thickness of microcapsule is 4～8 μ m.
3, according to the artificial hair mammilla substition structure of claim 1 or 2, it is characterized in that: the hair papilla cell behaviour scalp hair papilla cell in the microcapsule.
4, according to the artificial hair mammilla substition structure of claim 1 or 2, it is characterized in that: the hair papilla cell in the microcapsule is the animal wool papillose cell.
5, a kind of manufacture method of artificial hair mammilla substition structure is characterized in that comprising following key step successively: 1., hair papilla cell is suspended from the sodium alginate soln that concentration is 15～25g/L, form suspension, make in every milliliter of suspension to contain 1.5～9 * 10
5Individual hair papilla cell; 2., utilizing the high voltage electric field generator for microcapsules to make suspension that 1. step obtain form diameter is the micro-droplet status of 200～650 μ m, splashes into the CaCl that concentration is 9～14g/L then
2In the solution, obtain solid microsphere shape precipitate; 3., solid microsphere shape precipitate that 2. step is obtained joins in the Poly-L-Lysine Solution that concentration is 0.5～1.5g/L, acts on 8～20 minutes, obtains solid microsphere shape precipitate; 4., solid microsphere shape precipitate that 3. step is obtained joins in the sodium alginate soln that concentration is 1.5～2.5g/L, acts on 3～10 minutes, obtains solid microsphere shape precipitate; 5., solid microsphere shape precipitate that 4. step is obtained joins in the liquor sodii citratis that concentration is 0.03～0.07mol/L, acts on 5～20 minutes, obtains the microspheroidal precipitate of hollow structure; 6., hollow structure microspheroidal precipitate that 5. step is obtained cleans with normal saline, can obtain artificial hair mammilla substition structure.
6, according to the manufacture method of claim 5, it is characterized in that: above-mentioned steps 1. among, make in every milliliter of suspension to contain 4～6 * 10
5Individual hair papilla cell.
7, according to the manufacture method of claim 5 or 6, it is characterized in that: above-mentioned steps 1. before, earlier place vessel to cultivate hair papilla, the hair papilla cell of moving out in the hair papilla is gone down to posterity obtain the hair papilla cell of quantity amplification after cultivating, and then carry out above-mentioned steps 1..
8, according to the manufacture method of claim 5 or 6, it is characterized in that: in the above-mentioned manufacture method, the hair papilla cell behaviour scalp hair papilla cell of employing.
9, according to the manufacture method of claim 5 or 6, it is characterized in that: in the above-mentioned manufacture method, the hair papilla cell of employing is the animal wool papillose cell.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109136168A (en) * | 2018-08-03 | 2019-01-04 | 湖南大学 | A kind of acquisition methods of artificial papilla like cell |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946678A (en) * | 1988-04-01 | 1990-08-07 | Dodd Thomas L | Process of treating the scalp and hair |
| AU7746091A (en) * | 1990-04-17 | 1991-11-11 | Pherin Corporation | Methods of tissue implantation |
| FR2676649B1 (en) * | 1991-05-22 | 1994-02-25 | Lvmh Recherche | COSMETIC OR PHARMACEUTICAL COMPOSITION, ESPECIALLY DERMATOLOGICAL, FOR PROMOTING PIGMENTATION OF THE SKIN OR HAIR, CONTAINING A CYPERUS EXTRACT AND METHOD FOR THE PRODUCTION THEREOF. |
| JP2001302520A (en) * | 2000-04-19 | 2001-10-31 | Takahashi Akemasa | Method for regenerating hair by cultured hair papilla cell |
| WO2003022043A1 (en) * | 2001-09-06 | 2003-03-20 | Shiseido Company, Ltd. | Hair follicle-reconstitution system and animal carrying the same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109136168A (en) * | 2018-08-03 | 2019-01-04 | 湖南大学 | A kind of acquisition methods of artificial papilla like cell |
| CN109136168B (en) * | 2018-08-03 | 2022-03-08 | 湖南大学 | Method for obtaining artificial hair papilla-like cells |
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