JP2001302520A - Method for regenerating hair by cultured hair papilla cell - Google Patents

Abstract

PROBLEM TO BE SOLVED: To enable the own hair to be regenerated to a sufficiently satisfactory extent. SOLUTION: This method comprises collecting a tissue having a required largeness from the head part of a patient, separating a hair papilla from the hair follicle in the collected tissue, culturing and proliferating the cells from the hair papilla, and implanting the proliferated cells on a depilated position of the head part of the patient oneself from whom the tissue for the hair papilla cells is collected.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a hair regenerating method using cultured dermal papilla cells, which can surely regenerate self hair.

[0002]

BACKGROUND OF THE INVENTION Male pattern baldness is closely related to male hormones, is induced by genetic and environmental factors, and more than half of men have some symptoms. In particular, it is not difficult to imagine that these symptoms are accelerated due to the recent stress society and disorder of life style. In recent years, beauty treatments for the purpose of beauty, whitening, and slimming have become popular among women. For men, many hair care facilities have appeared and advertised. From these facts, general men's interest in hair is increasing socially, and there is a need for a method for reliably regenerating hair.

[0003] Conventional treatments for alopecia include those that promote hair regeneration by external treatment with a hair restorer or a hair growth agent containing an active ingredient, or by internal treatment and stimulation of residual hair follicles by physical stimulation or the like. So-called hair transplantation, which transplants the hair of an artificial object or another part of the subject together with the hair follicle, has been the mainstream.

[0004]

Further, each of these treatment methods has its own characteristics. In the case of treatment with external preparations or internal medicine, the patient can be easily treated at home, but the effect is not only satisfactory, but there are relatively few cases where complete hair regeneration is observed.

[0005] In the case of self-hair transplantation, remnant reactions do not occur because the remaining hair is appropriately collected, separated into strains, and then transplanted to a hair loss part. However, when there are limited healthy remaining hair follicles, there are cases where it is difficult to collect donors, and a satisfactory number of hairs cannot be regenerated. Also, the procedure of stock splitting takes a long time, during which the patient must wait.

[0006] The present invention has been made in view of the above points, and it is possible to achieve satisfactory hair regeneration, and to perform only a very small surgical procedure. An object of the present invention is to provide a hair regenerating method that requires only several visits and does not impose a great burden on a patient.

[0007]

SUMMARY OF THE INVENTION Accordingly, the gist of the present invention is to collect a tissue to a required size from a patient's head, separate a hair papilla from a hair follicle of the collected tissue, Thereafter, there is provided a method for regenerating hair by using cultured dermal papilla cells, which comprises culturing cells from the dermal papilla, proliferating the cells, and transplanting the dermal papilla cells to a hair loss site on the head of the patient who has collected the tissue.

Further, as described above, the present invention separates a hair papilla from a hair follicle of a patient, cultures cells from the separated hair follicles, and transplants the cells. No reaction occurs.

[0009] Further, if serum of a patient whose tissue is collected is used for culture of dermal papilla cells, there is no danger of serum-borne infections (viruses, bacteria, etc.).

[0010] Further, when the transplant is performed using a syringe, a required amount of injection can be easily performed at a required site without the need for surgery or the like.

Here, the theory of hair regeneration on which the present invention is based will be described. Hair papilla cells belong to mesenchymal cells and can be subcultured a plurality of times like skin fibroblasts. Once a scalp piece is collected, a large amount of cultured cells can be obtained. If 100 hair papilla are collected from 100 hair follicles for the first time, 15 × 10 ⁶ to 2 × 10 ⁷ cultured hair papilla cells can be secured after about one month.

[0012] Hair follicle tissue develops after 2-3 years of anagen, 2-3
After a week of regression, it enters a rest period of 3-4 months to halt hair growth. After this, the hair grows again and the hair growth is resumed. Although these hair cycles are maintained throughout life, it is said that in male pattern baldness, the period of anagen is shortened, so that the hair enters a catagen rest period before the hair shaft becomes thicker.

It is thought that the thickness of the hair shaft in the hair follicle tissue is determined by the hair papilla. In rats, the hair papilla, which has a large beard, is implanted under the auricle subcutaneous surface. The same thick and long hair as the beard can be regenerated in the thin and short hair in the middle.

It is known that the ability of the dermal papilla to induce hair is not limited to isolated dermal papilla but is also retained in cultured dermal papilla cells. That is, a large amount of hair papilla cells can be ensured to proliferate by cell culture technology, and a large number of hairs can be induced and reproduced.

Hair has remarkably different properties depending on the site where it grows. In the head hair, the frontal region and the parietal region are sites that cause male pattern baldness, and the hair follicles at these sites have undergone miniaturization under the influence of male hormones. In contrast, hair follicles in the temporal and occipital regions are not affected by male hormones, and often remain in temporary male pattern baldness patients.

In view of the fact that the hair papilla induces and regenerates the hair as described above, if cell culture is carried out from the occipital or whisker papilla, the hair follicles regenerated using these will not be miniaturized and will be healthy. It maintains the principle of donor dominant to maintain good growth.

As described above, hair papilla cells have hair-inducing ability, but these cells themselves control the proliferation of epithelial cells by producing and releasing cell growth factors.

It is considered that the hair papilla cells at the hair loss site decrease the production of these cell growth factors under the influence of androgens, or conversely release the growth inhibitory factors. Conversely, it is known that male hormones increase the production of cell growth factors including IGF-1 in beard hair follicles whose growth is significantly promoted by male hormones.

Considering these facts, if a site other than the site where hair loss occurs is used as a donor, a large amount of hair papilla cells capable of releasing some growth factors can be obtained in large quantities. By transplanting into a hair follicle, not only a new hair follicle can be induced, but also a growth factor may be provided to a miniature hair follicle to promote its growth. It is believed that this effect accelerates the recovery of the patient's hair.

[0020]

Embodiments of the present invention will be described below. The present invention is performed in the following order. (1) Separation of patient serum About 50 ml of blood is collected from a patient vein, and centrifuged to separate serum. (2) Collection of tissue from living body Local anesthesia is performed on the occipital region with 1% xylocaine. The size of the tissue to be collected is about 1 cm ² , and the size is the same as that used for tissue examination. In this case, scars after healing are hardly noticeable. (3) Separation of hair follicle The collected tissue is isolated under an operating microscope so as not to damage the hair follicle. The lowermost part of the hair follicle, called the bulb, is cut with a microsurgical scalpel, the connective woven part is inverted, exposing the dermal papilla. Here, the dermal papilla is cut off using a scalpel and moved to another culture dish prepared in advance. (4) Culture of hair nipples About 10 to 20 hair nipples are collected in a culture dish having a diameter of 3.5 cm, and gently sunk so as to adhere to the culture dish. The culture solution at this time is DMEM containing 10% of the patient's serum. Add non-essential amino acids, streptomycin, penicillin, and fungizone. These culture dishes were placed at 37 ° C., 5% CO ₂
Let stand for about 2 weeks under the environment. After 2 weeks, if cell growth can be confirmed under an inverted microscope, the culture medium is replaced.
At this time, the exchange frequency of the culture solution may be appropriately adjusted according to the number of cells. Usually about once every three days. Only when the cells have grown sufficiently during the observation, the cells are detached from the culture dish and suspended using 0.05% trypsin, a cell suspension is prepared, and subculture is performed. These cells undergo cell division and proliferate at a doubling time of 36 to 40 hours, so that passage is performed each time the cell density becomes too high. (5) Preservation of cells by liquid nitrogen After 3-4 passages, all cells were collected,
After suspending in a culture solution containing 10% patient serum containing 10% glycerol, the suspension is dispensed into approximately 1 × 10 ⁶ cells and stored in liquid nitrogen. In addition, the patient's serum is used as an additive in these series of processes, but since this is the patient's own serum, there is no risk of infection (virus, bacteria, etc.) via any serum, and preoperative examination. At the same time, if blood collection is performed, there is an advantage that technical complexity can be avoided.

(6) Transplantation of dermal papilla cells Transplantation instrument A 21G or 22G injection needle is held at the tip, and the body is 1-2.
It is a syringe that can freely convert a volume of 0 μl. FIG. 1 shows an example of the syringe.
Is an injection needle. The syringe body 1 adjusts the amount of liquid by operating part A. Injection method 1 vial about 1 × 10 ⁶ taken out of liquid nitrogen tank
The individual hair papilla cells were returned to 37 ° C., washed twice with DMEM, and evenly dispersed and suspended in 5 ml of DMEM. Then, the scale of the syringe was sucked up to 5 μl (containing about 1,000 hair papilla cells). Then, a needle is inserted into the epidermis under the epilation area which has been previously locally anesthetized, and the liquid is injected. The needle is inserted into the subcutaneous skin as a guide, and is set to 2 to 3 mm to prevent leakage of the infusate.
This operation is repeated a predetermined number of times (thousands if 1,000 places). Squeeze for several minutes after the end of the puncture, confirm that there is no significant bleeding, and end the procedure.

[0022]

According to the present invention, it is possible to sufficiently satisfactorily regenerate the hair. In addition, since only a very small surgical procedure is required, a treatment of about 30 minutes per year can be performed several times (about 5 times) and the patient is not burdened. In addition, since cells are cultured from the hair follicles isolated from the hair follicles of the patient themselves and transplanted, immunological rejection does not occur.

In addition, if serum of a patient whose tissue was collected is used for culturing hair papilla cells, there is no danger of serum-borne infections (viruses, bacteria, etc.).

In the case where the transplant is performed using a syringe, a required amount of injection can be easily performed at a required site without the need for surgery.

[Brief description of the drawings]

FIG. 1 is an explanatory view of a syringe used for transplantation in the present invention.

[Description of Signs] 1 Syringe body 2 Injection needle A Liquid volume adjustment scale

 ──────────────────────────────────────────────────続 き Continuation of the front page (71) Applicant 500179611 Sotaro Kurata 11-6 Moegidai, Oita City, Oita Prefecture (71) Applicant 597053670 Tetsuo Ezaki 1-7-21 Yoga, Setagaya-ku, Tokyo (72) Inventor Sotaro Kurata 11-6 Moegidai, Oita City, Oita Prefecture (72) Inventor Tetsuo Ezaki 1-7-21 Yoga, Setagaya-ku, Tokyo F-term (reference) AA04 BB48 CA04 MA66 MA67 NA05 NA14 ZA92

Claims (3)

[Claims] 1. A tissue of a required size is collected from a patient's head, a hair papilla is separated from a hair follicle of the collected tissue, and cells are cultured from the hair papilla and propagated. A hair regenerating method using cultured dermal papilla cells, wherein the cells are transplanted into a hair loss site on the head of a patient from whom the tissue was collected. 2. The method for regenerating hair using cultured dermal papilla cells according to claim 1, wherein the serum of the patient who has collected the tissue is used for culturing the dermal papilla cells. 3. The method for regenerating hair using cultured dermal papilla cells according to claim 1, wherein the transplantation is performed using a syringe.