CN1640291A - Dehydrated solid-state composite fermenting agent for apple dreg fodder protein fermentation and its producing method - Google Patents
Dehydrated solid-state composite fermenting agent for apple dreg fodder protein fermentation and its producing method Download PDFInfo
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- CN1640291A CN1640291A CNA2004100972368A CN200410097236A CN1640291A CN 1640291 A CN1640291 A CN 1640291A CN A2004100972368 A CNA2004100972368 A CN A2004100972368A CN 200410097236 A CN200410097236 A CN 200410097236A CN 1640291 A CN1640291 A CN 1640291A
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- leavening
- aspergillus niger
- saccharomycete
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Abstract
The present invention relates to a dehydrated solid compound fermentation agent for producing feed protein by means of solid fermentation of apple degs. The described dehydrated solid compound fermentation agent contains two or more than two microbions. The production method includes the following steps: uniformly mixing microzyme liquid fermentation agent (or wet microzyme body obtained by centrifugation or precipitation separation of microzyme fiquid fermentation agent) and solid culture of Aspergillus niger according to a certain ratio, low-temperature dehydration and drying so as to obtain the invented dehydrated solid compound fermentation agent.
Description
Technical field
The invention belongs to the fermented feed production technical field.Relate generally to a kind of composite ferment that is used for the pomace manufacturing feedstuff protein by solid state fermentation and preparation method thereof.
Background technology
Pomace is the solid-state castoff during cider is produced.China produces about 2,000 ten thousand tons of apple per year, annual about 1,000,000 tons of the pomace of discharging of apple processing.At present, except that a small amount of pomace is used as the feed, the overwhelming majority is not utilized effectively.Pomace water content height (70%～80%) contains the great amount of soluble nutriment, for microbial growth provides advantage, so it is smelly very easily to rot when fresh pomace is discarded, and serious environment pollution, and cause the wasting of resources.The main component of pomace is organic acid beyond insoluble carbohydrate (comprising pectin, cellulose, hemicellulose), soluble sugar and the aliphatic acid and NFE etc., can be for the carbon source and the energy abundance of growth of microorganism.In pomace, add suitable nitrogenous source, the part inorganic nitrogen can be converted into SCP, produce a large amount of B family vitamins and multiple enzyme simultaneously, improve the nutritive value of pomace through microbial fermentation.With the pomace is raw material, utilizes microbial fermentation manufacture order cell protein feed, to solving China's protein feed resources shortage, improving apple cultivation and processing industry benefit, reduces all significant and using value of environmental pollution.
Chinese patent " a kind of method of utilizing the pomace double-bacterium ferment to produce protein feeds " (publication number: CN1470181A; Open day: 2004.1.28; 2004 20 4 phases of volume of patent gazette) a kind of method of utilizing pomace to be rich in the fermented feed of SCP, B family vitamin and plurality of enzymes such as certain active cellulase, carbohydrase, pectase, phytase and protease by double-bacterium ferment production is disclosed.The weak point of this invention: the leavening manufacture craft of this invention is comparatively complicated, only is suitable for the enterprise that production scale is big, the leavening production equipment is complete and technical force is stronger.For not possessing the small business that makes qualified leavening condition, this invention has certain difficulty in force: the leavening investment of production equipment is bigger; Underproof leavening may cause fermented product quality instability, even produces the product of living contaminants.
The present invention proposes a kind of solid-state composite fermenting agent that contains two or more microorganism and production method thereof that is exclusively used in apple dreg fodder protein fermentation production, purpose is to simplify apple dreg fodder protein fermentation technology, solves small business's leavening making apparatus and is engaged in the difficulty of leavening technical force of production deficiency.Enterprise promptly strong by technical force, that special Zymolysis Equipment is arranged produces required leavening with apple dreg fodder protein fermentation and makes a kind of dehydrated solid-state composite fermenting agent finished product that contains multiple microorganism by special process, uses for pomace protein fermentation Feed Enterprise.
Summary of the invention
The present invention is to be the further improvement of the production technology of leavening in the patent of CN1470181A to publication number: mainly improve as follows: will the original saccharomycete liquid leavening that directly uses, can not transport at a distance and wet aspergillus niger solid state fermentation agent dehydration (mix the back dehydration or respectively the dehydration back mix) mix, make and can transport at a distance and the long-time dehydration composite ferment of preserving.Be about to mix by a certain percentage by the solid-state culture of the CN1470181A patent cultivation saccharomycetic liquid starter of gained or solid-state yeast thalline of saccharomycete liquid leavening centrifugation gained and aspergillus niger, the low temperature dewatering drying forms a kind of solid-state composite fermenting agent that contains capacity active dry yeasr bacterial cell and aspergillus niger spore.Pomace protein feed fermenting and producing enterprise directly buys this product, and the consumption by 4%～8% evenly mixes this product with fresh apple slag and required sterilization batching, adopts solid thick fermentation method heat-preservation fermentation can obtain qualified fermented product.The preparation process of this dehydration composite ferment is as follows:
1. saccharomycete liquid leavening preparation
Undertaken by CN1470181A patent technology: saccharomycete liquid culture medium (g/L): edible brown sugar 30 grams, K
2HPO
41.0 gram, MgSO
47H
2O 0.5 gram, (NH
4)
2SO
4, 2.0 grams add fresh pomace and soak juice (method for making: fresh pomace 300 gram adds water to the abundant stirring and evenly mixing of 1L, and slagging-off gets final product; Enlarge in this ratio when carrying out industrial production) to 1L, sterilization (120 ℃ * 20 minutes) and conventional liq submerged aerobic fermentation technology (cultivating 72 hours for 28 ℃) prepares saccharomycete liquid leavening routinely, and its saccharomycete bacterium number is (2.0～6.0) * 10
9Cfu/ml;
2. aspergillus niger solid state fermentation agent preparation
Undertaken by CN1470181A patent technology: culture medium, wheat bran: running water=1: 1, normal-pressure sterilization is 30 minutes behind the abundant mixing, be cooled to 30 ℃ of solid-state microbial inoculums 0.5% of inoculated aspergillus niger, place on the with a false bottom fermentation bed, thickness of feed layer 5～10cm, 28 ℃ of cultivations got final product in 72 hours.
3. apple dreg fodder protein is produced the solid-state composite fermenting agent preparation
Method 1: will mix by 1: 2 by the saccharomycete liquid leavening and the agent of aspergillus niger solid state fermentation of CN1470181A patent technology preparation, 35～40 ℃ of decompression dehydration dryings, product is pulverized pack and is got final product.
Method 2: prepare saccharomycete powdery leavening and the agent of aspergillus niger solid state fermentation respectively, saccharomycete powdery leavening after the drying and crushing and the abundant by a certain percentage mixing of aspergillus niger solid state fermentation agent are got final product.Concrete grammar is as follows:
To adopt centrifugal process or precipitation method separating thallus cell by the saccharomycete liquid leavening of CN1470181A patent technology preparation; With separating obtained wet thallus cell and starch in 1: 4～5 ratio mixing, 35～40 ℃ of decompression dehydration dryings, product is pulverized; The agent of aspergillus niger solid state fermentation is separately 35～40 ℃ of decompression dehydration dryings, and product is pulverized; Saccharomycete powdery leavening after the drying and crushing and the agent of aspergillus niger solid state fermentation are got final product by 1: 5～10 abundant mixings.
Method 1,2 compares: in method 1, the not centrifugal dehydration of saccharomycete liquid leavening, the low-temperature reduced-pressure drying need be taken off a large amount of moisture behind the very low aspergillus niger solid state fermentation product mixing of saccharomycete liquid leavening and water content, needs long time and more energy resource consumption.This method is applicable under the situation of no dehydration equipment and uses.In the method 2, the centrifugal dehydration of saccharomycete liquid leavening elder generation obtains a small amount of yeast cell, carries out drying under reduced pressure behind wet yeast thalline and the amount of starch mixing, and the water yield that need take off is few; Aspergillus niger solid state fermentation product water content is low, adopts the two independent dewatering process can save a large amount of time and the energy.In the enterprise that the yeast cells separation condition is arranged, the better effects if of method 2.
4. the use of solid-state composite fermenting agent: fresh apple slag, dregs of fat powder and raw materials such as urea or ammonium sulfate are cooperated and abundant mixing by a certain percentage, evenly insert in the fermentation of apple pulp raw material by 4%～10% consumption solid-state composite fermenting agent again the present invention's preparation, move on the band fermentation bed at the false end and spread out, about 25～the 30cm of thickness, insulation to 25 ℃～28 ℃ is cultivated oven dry in 48～72 hours and is pulverized and get final product.
The specific embodiment
Solid-state composite fermenting agent preparation example 1
Saccharomycete liquid leavening preparation: undertaken by CN1470181A patent technology: saccharomycete liquid culture medium (g/L); Edible brown sugar 30 grams, K
2HPO
41.0 gram, MgSO
47H
2O 0.5 gram, (NH
4)
2SO
4, 2.0 grams add fresh pomace and soak juice (method for making: fresh pomace 300 gram adds water to the abundant stirring and evenly mixing of 1L, and slagging-off gets final product; Enlarge in this ratio when carrying out industrial production) to 1L, sterilization (120 ℃ * 20 minutes) and conventional liq submerged aerobic fermentation technology (cultivating 72 hours for 28 ℃) prepares saccharomycete liquid leavening routinely, and its saccharomycete bacterium number is (2.0～6.0) * 10
9Cfu/mL;
Aspergillus niger solid state fermentation agent preparation: undertaken by CN1470181A patent technology: culture medium, wheat bran: running water=1: 0.9～1, normal-pressure sterilization is 30 minutes behind the abundant mixing, be cooled to 30 ℃ of solid-state microbial inoculums 0.5% of inoculated aspergillus niger, place on the with a false bottom fermentation bed, thickness of feed layer 5～10cm cultivates for 28 ℃ and got final product the aspergillus niger spore number in 72 hours: (6.0～10.0) * 10
9Cfu/g.
Apple dreg fodder protein is produced the solid-state composite fermenting agent preparation: will mix by 1: 2 by the saccharomycete liquid leavening and the agent of aspergillus niger solid state fermentation of CN1470181A patent technology preparation, and 35～40 ℃ of decompression dehydration dryings, product is pulverized pack and is got final product.
Solid-state composite fermenting agent preparation example 2
The preparation of saccharomycete liquid leavening: saccharomycete liquid is cultivated and is undertaken by preamble " solid-state composite fermenting agent preparation example 1 " technology; The saccharomycete system is equipped with: at the centrifugal 10min of 4000 commentaries on classics/min, the wet thallus yield is about 23g/L with saccharomycete liquid leavening.Saccharomycete wet thallus and starch are pressed 1: 4 abundant mixing, and the stand is to thin dish, and thickness is about 1～3cm, and 35 ℃ of low-temperature reduced-pressure dryings promptly get dry yeast bacterium powder after the pulverizing;
Aspergillus niger solid state fermentation agent preparation; Undertaken by preamble " solid-state composite fermenting agent preparation example 1 " technology; Aspergillus niger solid state fermentation product promptly gets the aspergillus niger spore powder 40 ℃ of low-temperature reduced-pressure dryings.
Dry yeast bacterium powder and aspergillus niger spore powder are promptly got the apple dreg fodder protein production solid-state composite fermenting agent that the present invention proposes in 1: 5 abundant mixing of ratio.
The result of use of this composite ferment: fresh pomace 300 grams, rapeseed cake powder 40 grams add urea to 2%, 120 ℃ sterilization 30min, the consumption by 5% inserts this leavening, cultivates 48 hours for 28 ℃, and the content of tunning pure protein is 240g/kg.
The major advantage of this composite ferment: easy to use, small business does not need investment buying leavening production equipment, need not join The know-how personnel that standby leavening is produced only need to press apple dreg fodder protein fermentation specification requirement proportioning raw material, by this leavening Consumption require evenly to insert this composite ferment, carry out again heat insulating culture and can finish sweat.
The fresh pomace water content is up to 80%, and the sugar content height is very easily putrid and deteriorated, can not transport for long-distance. In the pomace place of production just Ground processing is a kind of effective ways that reduce production costs. But because the preparation of pomace protein feed leavening needs special equipment And the technical staff, general small business is difficult to possess required condition, has directly affected fermentation deep processing and the resource of pomace. The research and production of this leavening will provide effective for fermenting and producing and the quality assurance of small business's pomace protein feed Hold.
Claims (5)
1. one kind is used for dehydrated solid-state composite fermenting agent of apple dreg fodder protein fermentation and preparation method thereof, it is characterized in that: contain two or more viable bacteria of saccharomycete and aspergillus niger in the dehydrated solid-state composite fermenting agent; Composite ferment passes through low temperature dewatering; Leavening is solid-state, can preserve and remote transportation in the long period (40～60 days); Can directly join and carry out solid state fermentation in the apple dreg fodder protein fermentation raw material; Two or more microorganism in the leavening all will be cultivated separately, then respectively or carry out low temperature dewatering behind the mixing.
2. according to the described dehydrated solid-state composite fermenting agent of claim 1, it is characterized in that leavening is made up of saccharomycete and two kinds of microorganism live bacterias of aspergillus niger, saccharomycete wherein is common Candida, aspergillus niger is aspergillus niger As3.4309 commonly used during food is brewageed; Two kinds are inserted bacterium and have the alternate effect in fermentation of apple pulp manufacture order cell protein processes.
3. according to the described dehydrated solid-state composite fermenting agent of claim 1, it is characterized in that the saccharomycete Inoculant in the leavening prepares by Liquid Culture.Fluid nutrient medium (g/L): edible brown sugar 30 grams, K
2HPO
41.0 gram, MgSO
47H
2O 0.5 gram, (NH
4)
2SO
4, 2.0 grams add fresh pomace and soak juice (method for making: fresh pomace 300 gram adds water to the abundant stirring and evenly mixing of 1L, and slagging-off gets final product; Get final product in this ratio expansion when carrying out industrial fermentation) to 1L, sterilization (120 ℃ * 20 minutes) and conventional liq submerged aerobic fermentation technology (cultivating 72 hours for 28 ℃) prepares saccharomycete liquid leavening according to a conventional method, and saccharomycete bacterium number is (2.0～6.0) * 10
9Cfu/mL; The agent of aspergillus niger solid state fermentation prepares with conventional solid-state culture technique: culture medium, wheat bran: running water=1: 0.9～1, normal-pressure sterilization is 30 minutes behind the abundant mixing, is cooled to 0.5%, 28 ℃ of cultivation of 30 ℃ of solid-state microbial inoculums of inoculated aspergillus niger and gets final product in 72 hours.
4. according to the described dehydrated solid-state composite fermenting agent of claim 1, it is characterized in that dehydrated solid-state composite fermenting agent can be by following two kinds of approach preparation: with saccharomycete liquid leavening directly and aspergillus niger solid state fermentation product mixed 35～40 ℃ of decompression dehydration dryings by 1: 2; Also can adopt centrifugal to saccharomycete liquid leavening earlier or precipitation method dehydration, again that gained is wet yeast cell body and starch mix by 1: 4～5,35～40 ℃ of following decompression dehydration dryings; The agent of aspergillus niger solid state fermentation is separately 35～40 ℃ of decompression dehydration dryings, and product is pulverized; Saccharomycete powdery leavening after the drying and crushing and the agent of aspergillus niger solid state fermentation are got final product by 1: 5～10 abundant mixings.
5. according to the described dehydrated solid-state composite fermenting agent of claim 1, it is characterized in that composite ferment is a particulate form, can directly insert and carry out solid state fermentation in the fermentation of apple pulp raw material that the access amount is 4%～8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100972368A CN1640291A (en) | 2004-11-17 | 2004-11-17 | Dehydrated solid-state composite fermenting agent for apple dreg fodder protein fermentation and its producing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100972368A CN1640291A (en) | 2004-11-17 | 2004-11-17 | Dehydrated solid-state composite fermenting agent for apple dreg fodder protein fermentation and its producing method |
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| Publication Number | Publication Date |
|---|---|
| CN1640291A true CN1640291A (en) | 2005-07-20 |
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|---|---|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101849610A (en) * | 2010-05-21 | 2010-10-06 | 王明堂 | Wrapped low-moisture rapid-orientation fermented straw nutrition forage grass |
| CN102028097A (en) * | 2009-10-06 | 2011-04-27 | 张宗舟 | Apple pomace bacterial protein production method |
| CN102550874A (en) * | 2012-01-18 | 2012-07-11 | 安徽燕之坊食品有限公司 | Method for producing active polypeptide rich product by solid state fermentation and application |
| CN112535253A (en) * | 2020-12-08 | 2021-03-23 | 江南大学 | Biological detoxification method for efficiently removing patulin in apple pomace |
-
2004
- 2004-11-17 CN CNA2004100972368A patent/CN1640291A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028097A (en) * | 2009-10-06 | 2011-04-27 | 张宗舟 | Apple pomace bacterial protein production method |
| CN101849610A (en) * | 2010-05-21 | 2010-10-06 | 王明堂 | Wrapped low-moisture rapid-orientation fermented straw nutrition forage grass |
| CN101849610B (en) * | 2010-05-21 | 2012-10-03 | 王明堂 | Wrapped low-moisture rapid-orientation fermented straw nutrition forage grass |
| CN102550874A (en) * | 2012-01-18 | 2012-07-11 | 安徽燕之坊食品有限公司 | Method for producing active polypeptide rich product by solid state fermentation and application |
| CN112535253A (en) * | 2020-12-08 | 2021-03-23 | 江南大学 | Biological detoxification method for efficiently removing patulin in apple pomace |
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