CN1632128A - Organic selenium from aspergillus oryzae and method for preparing same - Google Patents
Organic selenium from aspergillus oryzae and method for preparing same Download PDFInfo
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Abstract
The present invention discloses an organic selenium originating from aspergillus oryzae and method for making same and belongs to the alimentary products and health products additive agent field. The organic selenium in accordance with the present invention selects aspergillus oryzae at its carrier and exists in forms of aspergillus oryzae cell or aqueous solution or dry powder. The preparation method is that the selenium compound is selected as raw materials, then a series of steps are executed, such as selenium containing culture medium preparing, aspergillus oryzae inoculating and culturing, aspergillus oryzae collecting, selenium outside cells removing by wash, sterilization or/and drying; after drying, organic selenium in aspergillus oryzae cell form, or in aqueous solution form through thalli autolysis or in dry powder form by drying the aqueous solution. The aspergillus oryzae is characterized in that the bacterial develops fast and the content of the organic selenium inside selenium-rich rice aspergillus cells is high. During the fermentation process of aspergillus oryzae, inorganic selenium can be transformed into organic selenium which is easy to metabolize and absorb for human body. Organic selenium containing aspergillus oryzae takeing the place of common aspergillus oryzae to act as food product and health products additive agents s capable of trace-quantity compensating selenium continuously and can effectively prevent and cure diseases caused by absence of selenium.
Description
Technical field
The invention belongs to human food prods's additive agent field, relate to organoselenium that derives from aspergillus oryzae and preparation method thereof.
Background technology
Selenium is present in the multiple functional protein enzyme of human body.It is the component of Selenoperoxidase, phosphatide hydrogen Selenoperoxidase, 5 '-Tuo iodine enzyme active center key; participate in the metabolism of body endoperoxide and iodine; selenium has the free radical of removing, preventing cancer, anti-ageing beauty treatment, protection liver, opposing harmful heavy metal, radioprotective; regulate effects such as immunologic function, selenium is to hepatitis, cardiovascular diseases and the effect that all has obvious promotion to recover to the assisting therapy of the radiotherapy of tumour patient, chemotherapy.1973, The World Health Organization (WHO) was thought: selenium is trace element essential in human and animal's life, mends selenium and can effectively prevent multiple disease." dietary nutrients feed rate every day " of Chinese Soclety of Nutrition selenium has been classified as 15 kinds every day one of dietary nutrients.
Studies show that: the physiological requirements amount 40ug/d of selenium, boundary toxic dose 800ug/d, suggestion dietary supplementation amount 50ug/d～250ug/d, the highest safe intake 400ug/d, children are 150ug/d.International organizations such as WHO have all adopted these data.
Prevention now and treatment are because the disease main method that scarce selenium causes is manually to mend selenium.Used selenium mainly contains two kinds of forms: inorganic selenium and organoselenium.And the toxicity analysis revealed of selenium salt, inorganic selenium toxicity is stronger, generally only is used for pharmaceuticals, is not used in food, and its use range and metering all are restricted.By biology inorganic selenium is converted into organoselenium, not only can improves the physiologically active and the specific absorption of selenium, and can reduce its toxicity, just have general edible and health care and be worth.
Now main appliable plant and microorganism are changed inorganic selenium into organoselenium.Method for transformation is mainly: (1) microorganism is synthesized conversion method, as yeast rich in selenium, selenium-enriched edible mushroom etc.; (2) the synthetic conversion method of natural plant is as selenium-rich tea leaf, selenium-enriched apple, rich selenium algae etc.; (3) plant seed germination conversion method is as Fructus Hordei Germinatus rich in selenium, bean sprouts rich in selenium etc.Mainly by the synthetic conversion method preparation of microorganism, the microorganism of application has yeast and some edible funguses such as glossy ganoderma, mushroom etc. to the organoselenium of using now.These fungies also really are not deep in people's the three meals in a day, can not reach the purpose of mending selenium.
The prior art the most close with the present invention is that publication number is the patent of invention of CN1252835A, and name is called " having added food that derives from zymic selenium salt and the preparation method who derives from zymic selenium salt ".Its disclosed method that is used to produce selenium enrichment yeast product may further comprise the steps: the preparation nutraceutical mixture of yeast growth (water-containing medium); Then the selenium solution that obtains is filtered the selenium solution of preparation in distilled water by selenium compound being dissolved in the distilled water; Add this selenium solution in the yeast growth nutrition and mix and form a kind of selenium growth mixture; This selenium growth mixture is added in the yeast culture of living, form a kind of selenium yeast growth solution, and temperature is bathed under vibration; From the selenium yeast growth solution, reclaim and concentrated yeast cell; With the yeast cell washing of reclaiming, remove the outer selenium of born of the same parents; With yeast cell drying with washing.
Summary of the invention
The present invention provides a kind of aspergillus oryzae organoselenium and preparation method thereof that derives from.Derive from the aspergillus oryzae organoselenium for the crowd provides a kind of than the easier organoselenium salt that absorbs of inorganic selenium salt, overcome the shortcoming of direct benefit inorganic selenium.In foodstuffs industry is produced, replace common aspergillus oryzae with the aspergillus oryzae that contains organoselenium, make the product of its production, all contain organoselenium as soy sauce, vinegar, yellow rice wine etc., become a kind of protective foods of mending selenium.
Aspergillus oryzae is a kind of fungi commonly used in the foodstuffs industry, is used in the production to make soy sauce, the saccharifying agent of yellow rice wine, also is applied to fermentative production cellulase, amylase, polygalacturonase, kojic acid etc., and basis and using value are widely used.Discover that aspergillus oryzae and yeast, glossy ganoderma, mushroom be the same to have strong selenium rich ability.
The organoselenium that derives from aspergillus oryzae of the present invention is to be carrier with the aspergillus oryzae, and the form of existence is the organoselenium of exsiccant aspergillus oryzae cells form or aqueous solution form or dry powder form.
Organoselenium is content 5ug/ml～200mg/ml in the aspergillus oryzae aqueous solution.Content 10ug/mg～300ug/mg in aspergillus oryzae dry powder.
The organoselenium preparation method who derives from aspergillus oryzae of the present invention is a raw material with the selenium compound, and selenium compound comprises Sodium Selenite or/and sodium selenate passes through the process that preparation contains seleno culture medium, inoculation culture, collection washing, aqtocytolysis, sterilizes, makes finished product; It is characterized in that,
Said preparation contains seleno culture medium, is to be that the normal saline solution of 10mg/L～300g/L selenium compound joins fermented bean drink or/and in the aspergillus oryzae fermention medium of bean sprouts juice, form the contain seleno culture medium of the content of selenium compound at 10mg/L～10g/L with concentration;
Said inoculation culture, be with the aspergillus oryzae spore inoculating to containing in the seleno culture medium, 30～40 ℃ of temperature ranges, cultivated 15～90 hours;
Said collection washing is that the aspergillus oryzae cell that contains organoselenium is separated from contain seleno culture medium by centrifugal or filtration; With with the isoosmotic normal saline solution of aspergillus oryzae cell or D/W or phosphate buffered saline buffer with isolating aspergillus oryzae cell washing 2～12 times, remove the outer selenium of born of the same parents;
Said self-dissolving is to use high salt cell autolysis method, perhaps acid heat aqtocytolysis method, and lysing cell discharges nutritive substances such as organoselenium, amino acid, protein, ribose in the cell, makes the organoselenium of aqueous solution form.High salt cell autolysis method is to make the NaCl concentration in the self-dissolving system reach 1%～5%, temperature between 20 ℃～80 ℃, self-dissolving 5h～40h.Acid heat aqtocytolysis method be make the self-dissolving system the pH value in 2～7 scopes, temperature range is 20 ℃～70 ℃, self-dissolving 10min～30h regulates self-dissolving system pH to 6.3～7.3 with NaOH after self-dissolving is finished.
Said sterilization is to sterilize 15～30 minutes under 90～135 ℃ of temperature;
The said finished product of making is an organoselenium of making exsiccant aspergillus oryzae cells form; Make the aspergillus oryzae aqtocytolysis make the organoselenium of aqueous solution form; With aqueous solution drying, make the organic selenium product of dry powder form.
In inoculation culture, can be picking aspergillus oryzae spore direct inoculation in the bean sprouts medium that contains Sodium Selenite, 37 ℃, under 30～45 ℃ and 100～200rpm, cultivate 20～40h, turn out primary seed solution; Again primary seed solution is inoculated into according to 10% inoculum size and contains in the seleno culture medium, under 30～45 ℃ and 100～200rpm, cultivate 20～40h, as secondary seed solution; At last secondary seed solution is inoculated into according to 10% inoculum size and contains in the seleno culture medium.
Derive from the organoselenium preparation method of aspergillus oryzae in more detail, be divided into 7 steps:
(1) preparation can be supported the substratum of aspergillus oryzae growth.
(2) mother liquor of preparation selenium compound;
(3) the selenium compound mother liquor is added in the aspergillus oryzae fermention medium, make it to form a kind of substratum that contains selenium;
(4) inoculation and cultivation aspergillus oryzae make aspergillus oryzae fermentation growth by culture condition;
(5) from the aspergillus oryzae growth medium, collect the aspergillus oryzae cell;
(6), remove the outer selenium of born of the same parents with the aspergillus oryzae cell washing that reclaims;
(7) organoselenium of exsiccant aspergillus oryzae cells form is made in sterilization and/or dry; Make the somatic cells self-dissolving make the organoselenium of aqueous solution form; With aqueous solution drying, make the organic selenium product of dry powder form.
Preparation process of the present invention (1) aspergillus oryzae growth medium comprises the beans of bean sprouts medium, fermented bean drink substratum, rich in proteins, comprise soya bean, black soya bean, mung bean etc., cereal with rich in starch, comprise barley, wheat, corn etc., and byproduct is the liquid nutrient medium that main raw material is made, also comprising the substratum that can support the aspergillus oryzae growth, also can be that the mixture of above two or more substratum is formed.In substratum, add the VITAMIN to support the aspergillus oryzae growth, vitamin H, biological micromolecule material, organic and inorganic salt, mineral substance, elemental metals etc., these materials include but not limited to vitamin H, VITMAIN B1, vitamin B6, calcium pantothenate, inositol, copper, copper sulfate, zinc, zinc sulfate, iron, and ferric sulfate.Also add an amount of microbiotic in the substratum and prevent microbiological contamination, microbiotic comprises other microbiotic such as tsiklomitsin, Streptomycin sulphate.The pH of substratum is controlled between 3～9, and preferred range is controlled between 4～7.
Preparation process of the present invention (2) relates to the preparation of selenium compound mother liquor, and selenium salt such as selenium compound such as sodium selenate, Sodium Selenite are dissolved in the physiological saline, obtains the selenium mother liquor by filtration.Selenium compound comprises selenium salt or organic selenium compounds forms such as sodium selenate, Sodium Selenite.The selenium mother liquor contains the selenium compound of 10mg/L～300g/L.
Preparation process of the present invention (3) relates to the selenium compound mother liquor is added in the aspergillus oryzae fermention medium, makes it to form a kind of substratum that contains selenium.The content of final selenium compound is at 10mg/L～10g/L.
Preparation process of the present invention (4) relates to the inoculation and the cultivation of aspergillus oryzae.Vaccination ways comprises the seed liquor inoculation, uses the spore direct inoculation, the song inoculation of making when aspergillus oryzae is produced, but be not limited to above several inoculation method.Culture temperature is at 30 ℃～40 ℃, preferably at 35 ℃～38 ℃.Incubation time is 15h～90h, and preferable range is 40h～70h.When using liquid nutrient medium in process of production, cultivate on vibrator or shaking table, rotating speed is at 50rpm～200rpm, and preferable range is at 120rpm～170rpm.Producing that selected aspergillus oryzae can screen from environment in the rice rich in selenium aspergillus, can be directly to buy from the type culture collection center, also can be to separate in song used from industrial production or the fermented liquid.
Preparation process of the present invention (5) relates to collects the aspergillus oryzae cell from the aspergillus oryzae growth medium.The aspergillus oryzae cell that will contain organoselenium is separated from selenium aspergillus oryzae substratum by centrifugal or filtration.Centrifugal rotation speed is 1000rpm～10000rpm, and preferred version is at 4000rpm～8000rpm.Filtration can be selected methods such as filter paper, gauze, absorbent cotton, filter funnel, suction filtration for use.
Preparation process of the present invention (6) relates to the aspergillus oryzae cell washing that will reclaim, and removes the outer selenium of born of the same parents.With with the isoosmotic aqueous solution of cell with isolating aspergillus oryzae cell washing 2～12 times, be preferably 3～6 times, the key problem of washing soln is that the osmotic pressure with the aspergillus oryzae cell is consistent, and can reach best washing effect.The solute of the aqueous solution can be selected NaCl, KCl, MgSO for use
4, KH
2PO
4, Na
2HPO
4In inorganic salt, also can select biological micromolecule solution such as glucose, sucrose, maltose for use and select biological macromolecule solns such as polyoxyethylene glycol, polyvinyl alcohol, starch for use, the solute that can also use two or more is adjusted to the osmotic pressure of the aqueous solution presses consistent with the aspergillus oryzae Premeabilisation of cells.The concentration range of solute on the basis of itself and cell isotonic concentration downward modulation 90% to raising 200%.With NaCl is the composition of example explanation isotonic aqueous solution: waiting the concentration of oozing the NaCl aqueous solution is 0.9%, but the concentration range of NaCl can be 0.11%～2.7%, uses the aqueous solution in this scope all can reach washing effect preferably, effectively removes the outer selenium of born of the same parents.
Preparation process of the present invention (7) relates to aspergillus oryzae cell sterilization and/or dry, obtains the organic selenium product of exsiccant aspergillus oryzae cells form.Sterilization steps can be carried out at 90 ℃～135 ℃, preferably at 115 ℃～125 ℃.Drying is selected any dried forms such as seasoning, oven dry, vacuum-drying, freeze-drying for use, but is not limited to above several.Resulting dry rice aspergillus product contains the 10ug/mg that has an appointment～300ug/mg organoselenium.
The organoselenium of aqueous solution form: with the aspergillus oryzae aqtocytolysis of flush away extracellular selenium, lysing cell discharges nutritive substances such as organoselenium, amino acid, protein, ribose in the cell, makes the organoselenium of aqueous solution form.High salt cell autolysis method, perhaps acid heat aqtocytolysis method are adopted in the somatic cells self-dissolving.Filter insoluble substances such as removing cell walls after the somatic cells self-dissolving, with the material sterilization that obtains, sterilising temp is between 90 ℃～135 ℃, preferably between 115 ℃～125 ℃.Sterilization time is at 10min～2h, and the preferred time is at 20min～25min.The organoselenium that contains 5ug/ml～200mg/ml in the final aqueous solution organic selenium product.Salt in the high salt cell autolysis method is selected NaCl for use, adds the NaCl of solid form, and perhaps the NaCl with high concentration aqueous solution makes the NaCl concentration in the self-dissolving system reach 1%～5%, and preferred concentration is 2.5%～3.5%.The constant temperature self-dissolving, the temperature of self-dissolving can be between 20 ℃～80 ℃, and preferable range is between 50 ℃～60 ℃, and the self-dissolving time can be at 5h～40h, and the preferred time is 20h～30h.
Acid heat aqtocytolysis method is to add HCl in the aspergillus oryzae cell of selenium outside the flush away born of the same parents, the pH value that makes the self-dissolving system is in 2～7 scopes, preferred pH value is 3.5～4.5, temperature range is 20 ℃～70 ℃, preferred temperature is 40 ℃～50 ℃, the self-dissolving time is 10min～30h, and the preferred self-dissolving time is 30min～2h.After finishing, self-dissolving regulates self-dissolving system pH to 6.3～7.3 with NaOH.
The organoselenium of dry powder form: the organoselenium of the aqueous solution that obtains behind the aqtocytolysis being made dry powder form by means such as seasoning, oven dry, vacuum-drying, freeze-drying, preserve dry powder sterilization back, sterilising temp can carry out between 90 ℃～135 ℃, preferably at 115 ℃～125 ℃, sterilization time is 10min～2h, and the preferred time is 20min～25min.The organoselenium that contains 10ug/mg～300ug/mg in the dry powder.
Organoselenium of the present invention is carrier with the aspergillus oryzae, and aspergillus oryzae has strong selenium accumulation ability, and it is fast to have a growth, the high characteristics of organic selenium content in the rice rich in selenium aspergillus mycetocyte.In the method for the present invention, aspergillus oryzae can be transformed into organoselenium to the selenium of inorganic states at fermenting process, and this organoselenium is present in the aspergillus oryzae somatocyte, is human body institute's metabolism and absorption easily, is the selemium nutrition source of supplying with the human optimum that uses.Replace common aspergillus oryzae to be applied to during foodstuffs industry produces with the aspergillus oryzae that contains organoselenium, can provide a kind of method of easy, the trace element supplement selenium that continues for lacking the selenium crowd; As the additive of food and healthcare products, can reach trace, continue to mend selenium, effectively preventing is because of lacking the various diseases that selenium causes.
Embodiment
The concrete implementation step of the present invention is as follows, but and do not mean that limitation of the scope of the invention:
Embodiment 1
Obtaining liq bean sprouts medium at first.The Sodium Selenite mother liquor of refabrication 10g/L is drawn a certain amount of mother liquor and is joined in the bean sprouts medium, and the ultimate density that makes Sodium Selenite in the substratum is 150mg/L.Picking aspergillus oryzae spore direct inoculation is in the bean sprouts medium that contains Sodium Selenite, and 37 ℃, 150rpm are cultivated 24h, as primary seed solution; Primary seed solution is inoculated in the bean sprouts medium that contains Sodium Selenite according to 10% inoculum size, and 37 ℃, 150rpm are cultivated 24h, as secondary seed solution; Secondary seed solution is inoculated in the growth medium according to 10% inoculum size, and growth medium also is to contain the Sodium Selenite bean sprouts medium, and 37 ℃, 150rpm are cultivated 60h, 8000rpm, and centrifugal 30min collects the aspergillus oryzae cell.With physiological saline washing 3 times, the selenium compound that the flush away born of the same parents are outer, 121 ℃ of moist heat sterilization 20min.To go out the aspergillus oryzae cell of bacterium 60 ℃ of oven dry, made the organoselenium of exsiccant aspergillus oryzae cells form.
In embodiment 1, the culture temperature of primary seed solution and secondary seed solution can be cultivated 20～40h at 30～45 ℃, 100～200rpm.Culture effect is identical in above-mentioned temperature, rotating speed, time range.
Embodiment 2
Obtaining liq fermented bean drink substratum at first.The Sodium Selenite mother liquor of refabrication 5g/L is drawn a certain amount of mother liquor and is joined in the bean sprouts medium, and the ultimate density that makes Sodium Selenite in the substratum is 100mg/L.Picking aspergillus oryzae spore direct inoculation is in the bean sprouts medium that contains Sodium Selenite, and 37 ℃, 130rpm cultivate 20h, as primary seed solution; Primary seed solution is inoculated in the growth medium according to 10% inoculum size, and growth medium also is to contain the Sodium Selenite bean sprouts medium, and 30 ℃, 130rpm cultivate 90h, and 8 layers of filtered through gauze are collected the aspergillus oryzae cells.With D/W washing 12 times, the selenium compound that the flush away born of the same parents are outer adds solid NaCl and reaches 3%, 55 ℃ until concentration, self-dissolving 24h, and filter paper filtering is removed insolubless such as cell walls, 121 ℃ of moist heat sterilization 25min.Make the organoselenium of aqueous solution form.
Embodiment 3
Obtaining liq fermented bean drink substratum at first.The Sodium Selenite mother liquor of refabrication 50mg/L is drawn a certain amount of mother liquor and is joined in the bean sprouts medium, and the ultimate density that makes Sodium Selenite in the substratum is 10mg/L.Picking aspergillus oryzae spore direct inoculation is in the bean sprouts medium that contains Sodium Selenite, and 37 ℃, 100rpm cultivate 20h, as primary seed solution; Primary seed solution is inoculated in the growth medium according to 10% inoculum size, and growth medium also is to contain the Sodium Selenite bean sprouts medium, and 37 ℃, 100rpm cultivate 48h, and 8 layers of filtered through gauze are collected the aspergillus oryzae cells.With D/W washing 2 times, the selenium compound that the flush away born of the same parents are outer adds solid NaCl and reaches 2.5%, 50 ℃ until concentration, self-dissolving 30h, and filter paper filtering is removed insolubless such as cell walls, 121 ℃ of moist heat sterilization 25min.Make the organoselenium of aqueous solution form.
Embodiment 4
Obtaining liq fermented bean drink substratum at first.The Sodium Selenite mother liquor of refabrication 100g/L is drawn a certain amount of mother liquor and is joined in the bean sprouts medium, and the ultimate density that makes Sodium Selenite in the substratum is 5g/L.Picking aspergillus oryzae spore direct inoculation is in the bean sprouts medium that contains Sodium Selenite, and 37 ℃, 100rpm cultivate 20h, as primary seed solution; Primary seed solution is inoculated in the growth medium according to 10% inoculum size, and growth medium also is to contain the Sodium Selenite bean sprouts medium, and 40 ℃, 300rpm cultivate 15h, and 8 layers of filtered through gauze are collected the aspergillus oryzae cells.With D/W washing 10 times, the selenium compound that the flush away born of the same parents are outer adds solid NaCl and reaches 4%, 60 ℃ until concentration, self-dissolving 20h, and filter paper filtering is removed insolubless such as cell walls, 115 ℃ of moist heat sterilization 30min.Make the organoselenium of aqueous solution form.
Embodiment 5
Obtaining liq bean sprouts medium at first.The Sodium Selenite mother liquor of refabrication 15g/L is drawn a certain amount of mother liquor and is joined in the bean sprouts medium, and the ultimate density that makes Sodium Selenite in the substratum is 100mg/L.Some spore inoculatings of picking are in the bean sprouts medium that contains Sodium Selenite on the aspergillus oryzae bacterial classification of preserving, and 37 ℃, 150rpm cultivate 70h, and suction filtration is collected the aspergillus oryzae cell.With phosphate buffered saline buffer washing 3 times, the selenium compound that the flush away born of the same parents are outer adds HCl to pH and is worth 4.5,50 ℃, self-dissolving 1.5h, and 4000rpm removes insolubles, and supernatant is made dry powder with lyophilization, and 121 ℃, sterilization 20min.Obtain the organoselenium of dry powder form.
Embodiment 6
Obtaining liq bean sprouts medium at first.The Sodium Selenite mother liquor of refabrication 100mg/L is drawn a certain amount of mother liquor and is joined in the bean sprouts medium, and the ultimate density that makes Sodium Selenite in the substratum is 20mg/L.Some spore inoculatings of picking are in the bean sprouts medium that contains Sodium Selenite on the aspergillus oryzae bacterial classification of preserving, and 37 ℃, 150rpm cultivate 24h, and suction filtration is collected the aspergillus oryzae cell.With phosphate buffered saline buffer washing 2 times, the selenium compound that the flush away born of the same parents are outer adds HCl to pH and is worth 3.5,40 ℃, self-dissolving 1h, and 4000rpm removes insolubles, and supernatant is made dry powder with lyophilization, and 125 ℃, sterilization 20min.Obtain the organoselenium of dry powder form.
Claims (4)
1, a kind of organoselenium that derives from aspergillus oryzae is characterized in that, is to be carrier with the aspergillus oryzae, and existence form is the organoselenium of exsiccant aspergillus oryzae cells form or aqueous solution form or dry powder form.
2, according to the described organoselenium that derives from aspergillus oryzae of claim 1, it is characterized in that, in the aspergillus oryzae aqueous solution, contain the organoselenium of 5ug/ml～200mg/ml; Or in aspergillus oryzae dry powder, contain the organoselenium of 10ug/mg～300ug/mg.
3, a kind of preparation method of the organoselenium that derives from aspergillus oryzae of claim 1, with the selenium compound is raw material, selenium compound comprises Sodium Selenite or/and sodium selenate passes through the process that preparation contains seleno culture medium, inoculation culture, collection washing, aqtocytolysis, sterilizes, makes finished product; It is characterized in that,
Said preparation contains seleno culture medium, is to be that the normal saline solution of 10mg/L～300g/L selenium compound joins fermented bean drink or/and in the aspergillus oryzae fermention medium of bean sprouts juice, form the contain seleno culture medium of the content of selenium compound at 10mg/L～10g/L with concentration;
Said inoculation culture, be with the aspergillus oryzae spore inoculating to containing in the seleno culture medium, 30～45 ℃ of temperature ranges, cultivated 15～90 hours;
Said collection washing is that the aspergillus oryzae cell that contains organoselenium is separated from contain seleno culture medium by centrifugal or filtration; With with the isoosmotic normal saline solution of aspergillus oryzae cell or D/W or phosphate buffered saline buffer with isolating aspergillus oryzae cell washing 2～12 times, remove the outer selenium of born of the same parents;
Said aqtocytolysis is to use high salt cell autolysis method, perhaps acid heat aqtocytolysis method, and lysing cell discharges nutritive substances such as organoselenium, amino acid, protein, ribose in the cell, makes the organoselenium of aqueous solution form.High salt cell autolysis method is to make the NaCl concentration in the self-dissolving system reach 1%～5%, at 20 ℃～80 ℃ self-dissolving 5h～40h.Acid heat aqtocytolysis method is to make the pH value of self-dissolving system 2～7, and 20 ℃～70 ℃, self-dissolving 10min～30h regulates self-dissolving system pH 6.3～7.3 with NaOH after self-dissolving is finished.
Said sterilization is to sterilize 15～30 minutes under 90～135 ℃ of temperature;
The said finished product of making is an organoselenium of making exsiccant aspergillus oryzae cells form; Or make the aspergillus oryzae aqtocytolysis make the organoselenium of aqueous solution form; Or, make the organic selenium product of dry powder form with aqueous solution drying.
4, according to the described preparation method who derives from the organoselenium of aspergillus oryzae of claim 3, it is characterized in that, said inoculation culture, be that picking aspergillus oryzae spore direct inoculation is in the bean sprouts medium that contains Sodium Selenite, 37 ℃, under 30～45 ℃ and 100～200rpm, cultivate 20～40h, turn out primary seed solution; Again primary seed solution is inoculated into according to 10% inoculum size and contains in the seleno culture medium, under 30～45 ℃ and 100～200rpm, cultivate 20～40h, as secondary seed solution; At last secondary seed solution is inoculated into according to 10% inoculum size and contains in the seleno culture medium.
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| CN103923872A (en) * | 2014-04-17 | 2014-07-16 | 湖北工业大学 | Aspergillus oryzae activity enhancer and culture method of aspergillus oryzae |
| CN105907652A (en) * | 2016-06-30 | 2016-08-31 | 北京源生元科技发展有限公司 | Aspergillus oryzae strain YSY035 and isolating and screening method and application of selenium-enriched Aspergillus oryzae |
| WO2016134406A1 (en) | 2015-02-26 | 2016-09-01 | Dakota Star Capital, Llc | Mineral enriched natural supplements |
| CN109777742A (en) * | 2019-02-23 | 2019-05-21 | 湖北和诺生物工程股份有限公司 | A kind of separating screening method of selenium-rich aspergillus oryzae and its application |
| CN113396965A (en) * | 2021-06-17 | 2021-09-17 | 浙江工商大学 | Nutritional nano biological composite preservative, preparation method thereof and application of preservative to preservation of Taihu whitebait |
-
2004
- 2004-11-25 CN CNB2004100112554A patent/CN100548376C/en not_active Expired - Fee Related
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| CN103907937A (en) * | 2014-04-11 | 2014-07-09 | 湖北工业大学 | Soybean peptide nutrient solution and preparation method thereof |
| CN103907937B (en) * | 2014-04-11 | 2015-03-18 | 湖北工业大学 | Soybean peptide nutrient solution and preparation method thereof |
| CN103923872A (en) * | 2014-04-17 | 2014-07-16 | 湖北工业大学 | Aspergillus oryzae activity enhancer and culture method of aspergillus oryzae |
| CN103923872B (en) * | 2014-04-17 | 2015-11-18 | 湖北工业大学 | A kind of energetic agent of aspergillus oryzae and cultural method thereof |
| WO2016134406A1 (en) | 2015-02-26 | 2016-09-01 | Dakota Star Capital, Llc | Mineral enriched natural supplements |
| CN107427053A (en) * | 2015-02-26 | 2017-12-01 | 库拉全球健康(英属维尔京群岛)有限公司 | Natural supplement rich in mineral matter |
| US10863762B2 (en) | 2015-02-26 | 2020-12-15 | Cura Global Health (Bvi) Limited | Mineral enriched natural supplements |
| CN105907652A (en) * | 2016-06-30 | 2016-08-31 | 北京源生元科技发展有限公司 | Aspergillus oryzae strain YSY035 and isolating and screening method and application of selenium-enriched Aspergillus oryzae |
| CN109777742A (en) * | 2019-02-23 | 2019-05-21 | 湖北和诺生物工程股份有限公司 | A kind of separating screening method of selenium-rich aspergillus oryzae and its application |
| CN113396965A (en) * | 2021-06-17 | 2021-09-17 | 浙江工商大学 | Nutritional nano biological composite preservative, preparation method thereof and application of preservative to preservation of Taihu whitebait |
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