CN1623532A - Plant source acne fast detoxifying cleaning skin protecting emulsion and its preparation method - Google Patents

Abstract

A vegetative lotion for quickly treating acne and protecting skin and its perparing process are disclosed. Its advantages are quickly taking its effect (8hr), low cost and no pollution.

Description

Plant source acne quick detoxicating washing and skin care emulsion and its preparation method

The plant-derived acne quick detoxification washing and skin care cream and the preparation method in the technical field are biochemical technology and biochemical products which are comprehensively developed according to the composition and the physicochemical property of substances accumulated on the surface of an acne wound, the acne cause and the biotransformation pathway thereof and the substance composition, the biological function and the physicochemical property of purslane mucilage.

Background art acne diseases are one of the medical problems of dermatology at home and abroad. The treatment effect of various medicines and various washing cosmetics on acne is not very ideal.

Disclosure of Invention

Mechanism (a)

Acne can be divided into three types according to the composition of acne wound accumulation substances:

1. the pathological feature of acne of the molecule accumulation type of phosphoglyceride is the formation of a conical pimple with blackheads on the surface of the skin. It is squeezed out of the skin as a whitish-yellow plug. The main component of this plug is phosphoglyceride. Pure phosphoglyceride molecules are white waxy solids that tend to darken upon exposure to air, due to the oxidation of unsaturated fatty acids at the 2 nd carbon atom of the phosphoglyceride molecule in air to form peroxides, which in turn lead to the formation of peroxide polymers. Therefore, acne exposed to the outside of the hair follicle has a blackhead.

2. The pathological feature of the acne of the type with accumulation of lysophosphoglyceride molecules is that one or a plurality of conical blood papules or pustules are formed on the surface of the skin, which are painful, itchy and swollen. When the blood is squeezed out or absorbed for several days, a pimple with blackheads is deposited. Lysophosphoglycerides are a strong emulsifier and have a hemolytic effect, and high concentrations of lysophosphoglyceride molecules can cause cell membrane poisoning and damage cell membranes. (therefore, it is reasonable to refer to acneas blood poison in TCM.)

3. The pathological feature of this type of acne is that the squeezing out of the black head is a white-yellow mycelium, a metabolite of corynebacterium acnes that parasitizes in the hair follicles. The corynebacterium acnes harmful to the skin are characterized by diffusibility, intractable and long-term property.

According to the fact that the acne accumulation substances are phosphoglyceride molecules and lysophosphoglyceride molecules, the fact that the metabolic pathway of the phosphoglyceride in vivo is disordered can be judged. Because the phosphoglyceride molecule is a reactant of phosphoglyceride metabolism; lysophosphoglycerides are intermediates of phosphoglyceride metabolism, and these two compounds are now released as end products and accumulate on the skin surface, indicating a problem with the catalytic activity of certain enzymes in the enzyme system of phosphoglyceride metabolism. From the specificity of various phospholipases, it is possible to find out which phospholipases are catalytically inactive.

Phospholipase A₁Ester bond connected with 1 st carbon atom of glycerol molecule in single hydrolyzed phosphoglyceride molecule

Phospholipase A₂Ester bond connected with 2 nd carbon atom of glycerol molecule in specifically hydrolyzed phosphoglyceride molecule

Phospholipase C exclusively hydrolyzes ester bonds formed by glycerol and phosphoric acid

Phospholipase D exclusively hydrolyzes another ester bond formed by the polar head and the phosphate group

Phospholipase B hydrolyzes the complete phosphoglyceride molecule to neutralize C₁、C₂Two ester bonds connected

Lysophospholipase exclusively hydrolyzes ester bond to 1 st carbon atomin lysophosphoglyceride molecule

Phospholipase A₁A₂CD acting phosphoglyceride ester bond site phospholipase B acting on phosphoglyceride ester bond site

Lysophospholipase Effect lysophosphoglyceride ester bond site

The analysis of the specific hydrolysis sites and metabolic end products of various phospholipases as lysophosphoglyceride molecules and phosphoglyceride molecules, which are responsible for the production of a large number of lysophosphoglyceride molecules and phosphoglyceride molecules during the phosphoglyceride metabolism, indicates that phospholipase A₁No effect was achieved. But phospholipase A₁In the form of a proenzyme. The conversion from a proenzyme to an enzyme is effected by the induction of the enzyme. The proenzyme can only be activated when the inducer enzyme has catalytic activity. The DNA macromolecules are provided with genes for synthesizing various proteins required by human metabolism, and also comprise gene fragments for synthesizing various induction enzymes of a phospholipase system. Phospholipase A₁No catalytic activity, which indicates that the induced enzyme has no catalytic activity, and the induced enzyme has no catalytic activity, which indicates that the protein space structure has problems,the spatial structure of the protein is problematic, which indicates that phospholipase A is synthesized on the DNA molecule₁The enzyme-inducing gene fragment has a defect in nucleotide residues, and when the gene fragment is activated, mRNA is transcribed again from the defective gene fragment in a strictly A-U G-C base-pairing form. So that phospholipase A is synthesized in translation₁When the enzyme is induced, the molecular structure of the protein is bound with a defect, and the defect can cause that a certain secondary bond on the tertiary structure of the protein molecule can not be normally combined, and can not form an enzyme activity center, so that the catalytic activity of the protein is difficult to exert. Thus, phospholipase A₁Still in the zymogen phase. Phospholipase A₂Has catalytic activity, and can catalyze the hydrolysis of phosphoglyceride molecules to produce lysophosphoglyceride molecules. When the lysophosphoglyceride molecule in human tissue reaches a certain concentration, lysophospholipase antigen in pancreas and lysosome is activated, the ester bond connected with the 1 st carbon atom in the lysophosphoglyceride molecule is catalyzed and hydrolyzed to generate phosphatidic acid, and the phosphatidic acid enters a triacylglycerol and phosphoglyceride synthesis circulating system. However, the maximum synthesis amount of lysophospholipase is limited by the level of gene design, and can only meet the normal metabolic requirement of phosphoglyceride molecules. For coping with phosphoric acidThe enzyme concentration is apparently insufficient by excess lysophosphoglyceride molecules resulting from disturbances in glyceride metabolism. Phospholipase B has catalytic activity and is used for continuous hydrolysis of whole phosphoglyceride molecule C₁、C₂Two ester bonds above. It does not work for lysophosphoglyceride molecules with only one ester bond. Due to phospholipase A₁No catalytic activity, and breaks the relative equilibrium state of the phospholipase catalytic system for hydrolysis according to the shift. This transient imbalance also results in the liberation of a portion of the phosphoglyceride molecules. Free molecules of phosphoglycerides and excess lysophosphoglyceride molecules are gradually released from the sebaceous glands as end products of metabolism into the hair follicles and accumulate to form conical papules and blood papules.

The waxy phosphoglyceride molecules secreted from the sebaceous glands block hair follicles and open pores, so that corynebacterium acnes enter by gaps to infect parasitism. Corynebacterium acnes is a strongly infectious bacterium that produces a hyaluronidase enzyme during growth and reproduction. The pathogenic action of corynebacterium acnes is mainly based on the hydrolysis of aminopolysaccharide polymers by hyaluronidase to form aminooligosaccharides of tetrasaccharides or hexasaccharides. Due to the catalytic hydrolysis of hyaluronidase, the long-chain polymer structure of hyaluronic acid is destroyed, the hydrophilicity and the adhesive force are reduced, so that the intercellular space is enlarged, the connective tissue is loose, the immune defense function of the organism is reduced, and bacteria can be diffused and spread in the tissue and are harmful to the organism.

According to the three types of acne, the pathogenic substances are phosphoglyceride, lysophosphoglyceride and hyaluronidase, and the substances are biologically converted in vitro by utilizing the biological conversion reaction mechanism in the liver.

During the biotransformation reaction, uronic acid is one of the strongly polar binding substances and is an antidote in the liver, and toxic metabolites in vivo, non-nutritional wastes and chemical poisons such as food additives, medicines and pesticides entering the body are combined with uronic acid to generate strongly polar water-soluble uronic acid glycoside which can be discharged out of the body along with urine.

The purslane is rich in galacturonic acid, is circulating body fluid of the purslane in a normal state, is a galacturonic acid polymer with the molecular weight of about 15-30 ten thousand, and is mostly combined with calcium ions or magnesium ions to form carboxylate in the purslane body.

Extracting purslane galacturonic acid by using a biochemical technology.

Uronic acid binding is one of the most common of the biotransformation reactions. The compound containing polar groups such as alcohol, phenol, thiophenol, sulfhydryl, amine and carboxyl can be used as reactant to carry out combination reaction with uronic acid to generate glucuronide.

According to the biotransformation combined reaction mechanism, the purslane galacturonic acid is combined with acne pathogenic substances to carry out transformation detoxification treatment.

1. The purslane galacturonic acid and the phosphoglyceride molecules are combined to form the phosphoglyceride molecules, are fat-soluble substances, have low polarity, are insoluble in water, and form micelles in water. Various phosphoglycerides contain hydroxyl, amino, or carboxyl groups in the polar head, and these polar groups can be combined with galacturonic acid to form galacturonan.

2. The combination of purslane galacturonic acid and lysophosphoglyceride is a strong emulsifier, and has hemolytic effect, which poisons cell membrane and destroys cell membrane. A free hydroxyl group is bonded to the 2 nd carbon atom in the lysophosphoglyceride molecule; the polar head also contains amino, hydroxyl or carboxyl, and these polar groups can combine with galacturonic acid to form galacturonic acid glycoside.

Galacturonic acid + lysophosphoglyceride → galacturonic acid glycoside

3. The combination of purslane galacturonic acid and hyaluronidase catalyzes the hydrolysis of hyaluronic acid. High concentrations of hyaluronidase disrupt the aminopolysaccharide polymer structure that makes up the skin. The viscosity and water absorption capacity of hydrolyzed hyaluronic acid are greatly reduced, the intercellular space is enlarged, the permeability is increased, and the immune defense function of skin is reduced. Hyaluronidase is a protein. The hydrophobic groups of the protein molecules are mostly gathered in the molecule; the hydrophilic groups are distributed on the surface of the molecule. Secondary bonds are formed among the polar groups, and the active center of the enzyme is formed on the surface of the molecule. Galacturonic acid molecules are strongly polar binding substances, and the force on the polar groups on the surface of the protein molecule exceeds the attractive force between secondary bonds. When the galacturonic acid molecules contact the polar groups on the surface of the protein molecule, each secondary bond is broken and recombined with the galacturonic acid molecule to form a complex of galacturonic acid glycoside and protein polypeptide chain. In this way, the spatial structure of the protein molecule is destroyed, resulting in denatured inactivation of hyaluronidase. The pathogenic hyaluronidase is inactivated, and various phagocytes in lysosomes in vivo phagocytize and destroy corynebacterium acnes parasitizing in skin, and then the aminopolysaccharide rapidly proliferates, which can promote proliferation of fibers in matrix and promote healing of acne wound.

Galacturonic acid + hyaluronidase → protein polypeptide chain-galacturonic acid glycoside complex

Acne is a genetic metabolic disease caused by gene defects, is an expression mode of gene defects, has the root of diseases in vivo, and develops infinite metabolic poisons which need comprehensive treatment from the inside to the outside of the body. The cause of acne is due to genes, but this problem can be solved according to the biotransformation reaction of substances in the liver. It is suitable for foods rich in galacturonic acid, such as fresh herba Portulacae, herba Braseniaeel, Agaricus campestris, fructus Citri Tangerinae, fructus Mali Asiaticae, pectin, etc. The intake of spicy food such as scallion, garlic, pepper, wine, artificially synthesized bezoar, artificially synthesized borneol, pesticide on the surface of fruits and vegetables, etc. is strictly controlled. Since these substances directly stimulate the hypothalamus to secrete and release neurohormones such as corticotropin-releasing hormone and gonadotropin-releasing hormone. These two peptide hormones promote the secretion of excessive male hormone from adrenal gland and gonad, and these male hormones act on cell nucleus to make the nonspecific repressor protein on the mask-protected DNA molecule fall off, so that the gene is activated, transcribed, translated, synthesized and metabolized to produce acne matter. Galacturonic acid can be combined with excessive androgen in vivo and inactivated, or combined with non-nutrient substances such as toxic metabolites and metabolic waste in vivo and toxic compounds such as food additives, medicines and pesticides entering into body, converted into nontoxic water-soluble galacturonic acid glycoside, and discharged out of body with urine.

Purslane galacturonate emulsification mechanism purslane mucilage is circulating body fluid under the normal physiological state of purslane, has the molecular weight of about 15-30 ten thousand, and contains D-galacturonic acid, L-arabinose, D-galactose and the like in molecules. Most of the carboxyl groups in the D-galacturonic acid molecules are combined with calcium and magnesium ions to form carboxylate in the purslane body. The carboxylate has emulsifiability. Therefore, galacturonate in purslane is a natural emulsifier. Purslane galacturonic acid lengthThe chaincarboxylate molecules are in a zwitterionic state under the polarization of water molecules, and have hydrophobic non-polar ends and hydrophilic, negatively charged (-COO)^-) Polar ends, each of which prominently exhibits its own dissolution behavior. The oil on the skin surface is a hydrophobic substance, and the hydrophobic oil droplets tend to merge when they encounter water to form an aqueous layer and an oil layer. When the carboxylate molecules of galacturonic acid are present in water, according to a similar mutual solubility rule, the non-polar end of the long chain galacturonic acid carboxylate molecule dissolves into the center of the oil droplet on the hydrophobic skin surface, leaving its negatively charged carboxy end protruding into the surrounding water layer. Due to the mutual repulsion between the similar negative charges carried by the carboxyl groups on the long-chain molecules, the small oil drops can not be combined to form a stable emulsion of water and oil to be dissolved in water, thereby achieving the purposes of emulsification and purification.

The emulsification steps of the purslane galacturonate are as follows:

1、

namely:

2、

namely:

3、

namely:

n negatively charged (RCOOR '' RCOO^-) The long molecular chains repel each other and are dispersed in water, so that the long molecular chains are difficult to agglomerate together to form an oil layer to form a stable emulsion.

(II) method for preparing plant source acne quick detoxicating washing skin care milk

And (4) harvesting purslane within 6-9 months, shearing the overground part of the purslane by using scissors, and then slightly dropping the purslane seeds on the ground to return the purslane seeds to the field directly. Carefully selecting purslane, and picking off rotten branches and leaves and wounded branches and leaves.

Weighing 2000 g of purslane plant, cleaning with tap water, putting the cleaned purslane into a cleaned 40-mesh nylon gauze bag, putting the purslane into a spin dryer to remove surface clear water, cleaning a pulverizer with detergent and tap water, sterilizing with 75% alcohol, fastening a discharge port of the pulverizer with the 40-mesh nylon gauze bag, blowing away fragments of the purslane by wind power of the pulverizer, putting a clean plastic basin below the gauze bag, repeatedly crushing the weighed purslane until defoaming can be achieved by hands to form sticky masses, pulping the crushed purslane fragments by a beater, pouring purslane pulp into a double-layer 40-mesh nylon gauze bag, carrying out filter pressing, removing residues, pouring purslane mucilage into a double-layer 80-mesh nylon gauze bag, carrying out filter pressing, removing residues, pouring the purslane mucilage obtained by twice filtration into a ground bottle, covering tightly, putting the ground bottle into a refrigerator at 1-4 ℃, standing for 14 hours, centrifuging the defoamed purslane mucus for 5 minutes at 4000 rpm by using a centrifugal machine, taking supernatant, quickly packaging, sealing by using 10 ml/bag and hot pressing, or sealing by using 100 ml/bottle, sealing the bottle mouth, and storing the lactic acid bacteria in a refrigerator at 1-4 ℃. 400 ml of purslane mucus can be extracted from 2000 g of purslane plants.

(III) plant source acne quick detoxification washing and skin care milk product

The characteristics are as follows: brown translucent mucus, miro-acid

Raw materials: purslane plant 2000 g

Acne detoxification chemicals: purslane galacturonic acid,

Emulsifying, skin care chemicals: purslane galacturonate and purslane galacturonic acid mixture

The function is as follows: acne detoxification, emulsification and purification

The invention solves the problem of skin poison caused by acne by biochemical reaction mechanism, and achieves the purpose of emulsifying, detoxifying, preventing and protecting skin by biochemical products. The whole preparation process has the advantages of rich and easily obtained raw materials, low cost, simple process and no pollution, the leftovers are still biological substances, can be directly returned to the field as soil nutrients, meet the requirements of environmental protection and green products, and are suitable for production of various enterprises.

Fourth, detailed description of the invention

The plant-derived acne quick detoxification washing and skin care cream can be used for washing face, bathing, washing hair and caring skin. The acne treatment method is as follows:

1. method for treating acne with glycerophosphate

Gently squeezing out acne with blackheads on the face, pinching the tip epidermis with nails on papules without blackheads, carefully pouring washing and skin-care lotion into a small dish of 2ml, because purslane mucus has high specific gravity and issmooth and easy to slide to the ground, dipping the skin-care lotion on the whole face with fingers to be uniformly smeared thickly, causing killing and itching, repeatedly rubbing with hands, washing with tap water after 2 minutes, pouring 2ml for the same treatment, washing cleanly after 2 minutes, wiping off facial water, pouring 1ml of washing and skin-care lotion, dipping the skin-care lotion on the whole face with fingers to be uniformly smeared, causing killing and itching, and gradually adapting to the face after rubbing with hands for a few times, wherein the washing and skin-care lotion is a biological product without toxic and side effects, and acne embolism can be contracted into a hard knot after 8 hours, is easily stripped from skin, and treated once in the morning and evening, and the acne can be cured after 5 days without scar left.

2. Method for treating hemolytic phosphoglycerate type acne

Cleaning both hands, pinching the blood dune and the purulent blood dune of the face with nails to break the epidermis at the tip, sucking the blood stain with a facial tissue, carefully pouring the washing and skin-care lotion into a small dish of 2ml, dipping the washing and skin-care lotion with fingers to uniformly spread the whole face thickly, giving a feeling of killing and itching, rubbing the face repeatedly with both hands, washing the face cleanly with tap water after 2 minutes, pouring 2ml of the washing and skin-care lotion for the same treatment, washing the face cleanly after 2 minutes, wiping the face to dry, pouring 1ml of the washing and skin-care lotion, uniformly spreading the washing and skin-care lotion with hands to the whole face, giving a feeling of killing and itching, rubbing the face lightly with both hands for several times, after 8 hours, contracting the acne wound surface into a dry scab, removing the scab, treating the scab once in the morning and evening, removing the scab before each treatment, after 6 days,removing the scab, healing the acne, and leaving no scars.

3. Treatment method for corynebacterium acnes infected acne at phosphoglyceride accumulation site

Gently squeezing mycelium with blackheads from hair follicles, carefully pouring washing and skin care cream into a small dish of 2ml, dipping the skin care cream by hands to uniformly coat the whole face thickly, causing the skin care cream to be killed and itchy, repeatedly rubbing and rubbing by hands, washing the skin care cream by tap water after 2 minutes, pouring 2ml of the washing cream for the same treatment, washing the face by tap water after 2 minutes, drying the moisture of the face, pouring 1ml of the washing and skin care cream to coat the whole face, forming irregular yellow crystal particles by the corynebacterium acnes after 8 hours, rolling off the corynebacterium acnes from the hair follicles, or shrinking into a dry induration, easily peeling off the corynebacterium acnes from the skin, treating the corynebacterium acnes once in the morning and evening, and after 6 days, and recovering the acnes without scars.

The botanical acne fast detoxicating washing and skin protecting milk is biochemical product without any additive, and after mucus is taken out each time, the cap should be covered tightly, the bottle mouth is covered with plastic film and then tied tightly with rubber band to avoid infecting lactic acid bacteria, and the product is stored in refrigerator at 1-4 deg.c.

Experiments prove that the botanical acne quick detoxification washing and skin care cream has other effects.

1 radical cure of beriberi

Breaking large area of tinea pedis blister with needle, sucking water, applying washing and skin caring cream on the whole foot, rubbing repeatedly, relieving itching after 5 min, washing with tap water, applying a layer of washing and skin caring cream, treating in the morning and evening once, and treating 4 days later to recover tinea pedis without recurrence.

2. Removing wart

After the wart growing nearly one year is treated by washing and skin-care milk for one month, a hard cocoon is formed on the surface of the wart, and the cocoon is healed after self-shedding without scars.

3. The washing and skin-care lotion also has the following uses

(1) The toothpaste can eliminate peculiar smell in the oral cavity and treat gingivitis, and the teeth can show the luster of enamel after brushing.

(2) After bathing, the whole body is coated with a layer, and people can learn that mosquitoes do not bite in summer under the lamp.

(4) The scallion flavor, garlic flavor and fishy smell can be eliminated by brushing the chopping board.

(5) Can remove chelate formed by hair dyeing curium ointment and facial skin

4. Treating atheroma

The length multiplied by the width multiplied by the height multiplied by 2.7cm multiplied by 1cm multiplied by 1.2cm is grown for 20 years, the purple-black powder tumor with smooth surface is formed, after the powder tumor is smeared and washed and skin-care milk is applied for one month, the tissue bulge disappears and forms a plane with the surrounding skin, the purple-black color becomes the color of the muscle, the color of the normal skin is locally restored, and no pain or itch is caused.

The whole herb of purslane is used as a Chinese medicine for treating bacillary dysentery, poisonous insect and snake bite, and is also used as a medicine for psoriasis and a patch for various malignant sores. "" in bacteria with strong infectivity, in rapidly growing malignant tumors, in bee venom, snake venom, mosquito venom contains hyaluronidase, which can cause hyaluronic acid decomposition. "" several snake venoms contain Ca as required⁺⁺Phospholipase A of₂". Experiments prove that the purslane galacturonic acid can cure acne, atheroma, beriberi, wart and the like. Comprehensive analysis and theoretical inference: hyaluronidase and phospholipase A for treating malignant tumor with purslane galacturonic acid to promote growth of malignant tumor₂Allosterically inactivating, combining with lysophosphoglyceride to generate galacturonide, detoxicating, dissolving in water, and discharging with urine. Hyaluronidase and phospholipase A with pathogenic effect on malignant tumor₂Allosterically inactivated by phospholipase A₂The toxin produced by catalytic hydrolysis is also detoxified and excreted in vitro, and the malignant tumor loses the ability to survive and should be inactivated. It is expected that the treatment of malignant tumors with non-toxic portulacacuronic acid in a biotransformation-binding reaction will replace the treatment of malignant tumors with chemicals that inhibit their growth in the near future.

Claims (3)

1. A plant-source acne quick detoxification washing and skin-care emulsion and a preparation method thereof belong to biological washing cosmetic products and biochemical technology
2. 1. Method for preparing plant source acne quick detoxicating washing skin care milk

   And (4) harvesting purslane within 6-9 months, shearing the overground part of the purslane by using scissors, and then slightly dropping the purslane seeds on the ground to return the purslane seeds to the field directly. Carefully selecting purslane, and picking off rotten branches and leaves and wounded branches and leaves.

   Weighing 2000 g of purslane plant, cleaning with tap water, putting the cleaned purslane into a cleaned 40-mesh nylon gauze bag, putting the purslane into a spin dryer to remove surface clear water, cleaning a pulverizer with detergent and tap water, sterilizing with 75% alcohol, fastening a discharge port of the pulverizer with the 40-mesh nylon gauze bag, blowing away fragments of the purslane by wind power of the pulverizer, putting a clean plastic basin below the gauze bag, repeatedly crushing the weighed purslane until defoaming can be achieved by hands to form sticky masses, pulping the crushed purslane fragments by a beater, pouring purslane pulp into a double-layer 40-mesh nylon gauze bag, carrying out filter pressing, removing residues, pouring purslane mucilage into a double-layer 80-mesh nylon gauze bag, carrying out filter pressing, removing residues, pouring the purslane mucilage obtained by twice filtration into a ground bottle, covering tightly, putting the ground bottle into a refrigerator at 1-4 ℃, standing for 14 hours, centrifuging the defoamed purslane mucus for 5 minutes at 4000 rpm by using a centrifugal machine, taking supernatant, quickly packaging, sealing by using 10 ml/bag and hot pressing, or sealing by using 100 ml/bottle, sealing the bottle mouth, and storing the lactic acid bacteria in a refrigerator at 1-4 ℃. 400 ml of purslane mucus can be extracted from 2000 g of purslane plants.
3. 2. Plant source acne quick detoxification washing and skin care emulsion product

   The characteristics are as follows: brown translucent mucus, miro-acid

   Raw materials: purslane plant 2000 g

   Acne detoxification chemicals: purslane galacturonic acid,

   Emulsifying, skin care chemicals: purslane galacturonate and purslane galacturonic acid mixture

   The function is as follows: acne detoxification, emulsification and purification