CN1616062A - Health wine for strengthening immunity and its preparing method - Google Patents
Health wine for strengthening immunity and its preparing method Download PDFInfo
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- CN1616062A CN1616062A CN 200410080510 CN200410080510A CN1616062A CN 1616062 A CN1616062 A CN 1616062A CN 200410080510 CN200410080510 CN 200410080510 CN 200410080510 A CN200410080510 A CN 200410080510A CN 1616062 A CN1616062 A CN 1616062A
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Abstract
The present invention relates to a kind of health wine for strengthening immunity, and belongs to the field of health article technology. The health wine is prepared with American ginseng, ophiopogon root, chitin and white spirit as material. The health wine has the function of strengthening immunity and has the color, fragrance and flavor of the white spirit preserved.
Description
Technical field
The invention belongs to the technical field of health product, more particularly, the present invention relates to a kind of health promoting wine of enhancing immunity.
Background technology
Chinese liquor history is of long standing and well established, becomes indispensable a kind of beverage in people's life.Along with development of history, the progress in epoch, people's living standard improves constantly, and idea is also being brought in constant renewal in, and when requiring to drink, can not only enjoy drink happy, and will guarantee the health of health.Treasure life, pay close attention to the healthy widespread consensus that becomes people.People are invaded and sick by extraneous antibacterial why easily in the life, and main cause is that immunity function own lowly causes, and scientific and reasonable enhancing diet can improve autoimmune function effectively, and prevent disease takes place.In the prior art, in having the health promoting wine of health-care effect, mostly be in pure white wine, to soak and form by plurality of Chinese, though can reach its health-care effect, but great changes have taken place in its color, taste, and wine body inaccurate coordination loses the exclusive style of original Chinese liquor.
Summary of the invention
The present invention is for the problem of the color, taste and the style that solve health promoting wine lose original wine when reaching enhancing immunity, and having proposed a kind of is the health promoting wine of raw material with Radix Panacis Quinquefolii, chitin, Radix Ophiopogonis, Chinese liquor.
Concrete technology contents is as follows.
A kind of health promoting wine of enhancing immunity is made by the raw material of following weight portion: 8～12 parts of Radix Panacis Quinquefoliis, 25～35 parts of chitins, 3～7 parts of Radix Ophiopogonis, 450～550 parts of Chinese liquor.
Health promoting wine of the present invention is preferably made by the raw material of following weight portion: 10 parts of Radix Panacis Quinquefoliis, 30 parts of chitins, 5 parts of Radix Ophiopogonis, 480 parts of Chinese liquor.
The number of degrees of described Chinese liquor are preferably 30～60.
A kind of preparation method of health promoting wine of the present invention may further comprise the steps:
(1) Radix Panacis Quinquefolii and Radix Ophiopogonis are pulverized, crossed 20 purpose sieves;
(2) add at Radix Panacis Quinquefolii with in Radix Ophiopogonis Radix Panacis Quinquefolii and Radix Ophiopogonis the weight sum the mass percent concentration of 4～6 times of weight be 50% ethanol, under 95 ℃ temperature, refluxed 1～3 hour, filter with the rotating speed of 400 purpose filters with 3000rpm, obtain extracting solution, repeat again to extract and filter merge extractive liquid, 2 times;
(3) extracting solution that merges being evaporated to relative density is 1.35 o'clock, obtains extractum;
(4) with extractum, chitin and white spirit mixed preparation, obtain described health promoting wine.
Described Chinese liquor can have been bought from the Red Star seal wine general factory.Described chitin derives from the triumphant sharp biological preparation products factory in Guangzhou, and grade is the high-quality grade.
Functional component and content: every 100ml contains the total Saponin of 50～70mg.
Using method and consumption: every day 1 time, each 50ml.
Suitable crowd is the immunocompromised person, and the crowd that is not suitable for is children.
Shelf-life is 2 years.
Health promoting wine of the present invention has the effect of enhancing immunity.
Radix Panacis Quinquefolii (panaxquinguefolium) is famous and precious tonic, mainly originates in the U.S., Canada, and China's subcultivation is called Radix Panacis Quinquefolii, prescription Radix Panacis Quinquefolii by name, Radix Panacis Quinquefolii, Radix Panacis Quinquefolii.Main functional component is (R
0R
B1R
B3R
cR
dR
eR
fR
G1R
G2R
G3R
H1R
Ao) etc.Pharmacological testing shows that the ginsenoside has anti peroxidation of lipid, obviously increases the number of M cholinoceptor in the brain.Improve memory, promote protein synthesis, regulate multiple important biomolecule functions such as immunity.
Radix Ophiopogonis, (ophiopogon japoninicus) contained functional components such as multiple steroid saponin.Pharmacological testing shows to have following function Radix Ophiopogonis: leukocyte increasing, prolong the antibody life period, and improve immunologic function and nucleic acid synthetic ratio, enhancing antibody, complement, immune materials such as interferon, lysozyme produce.
Chitin (chtin) is the natural polymer polysaccharide from Crustacean; mainly from the shell of marine organisms Eriocheir sinensis and shrimp, extract; the water-soluble chitosan (chitosan) that is called of energy behind the chitin deacetylase base; it can reduce cholesterol in the blood; regulating the best acid-base value of body fluid is 7.4 at pH; activating human body cells, pair cell have induces and repair the enhancing human body immunity effect.
Health promoting wine of the present invention entrusts the disease prevention and control center, Hunan Province to check, and confirms to have following function and characteristics.
Health promoting wine of the present invention has the enhancing immunity function.
1 material and method
1.1 sample: the health promoting wine of the embodiment of the invention 1, be transparency liquid, the human oral recommended dose is 50ml/ people/sky, provides 5 times of concentrated solutions to be for experiment, amounting to the human oral recommended dose is 10ml (concentrated solution)/people/sky.
1.2 laboratory animal and grouping: 120 of cleaning level Male Kunming strain mice, body weight is 18g-22g, is provided by the laboratory animal department of the Chinese Academy of Sciences of Central South University, approval card number moves word 20-011 number for the Hunan Province doctor.Per 40 are divided into 1 group, totally three groups.Immunity I group is carried out carbon and is cleaned up experiment; Immunity II group is carried out dirty body ratio measurement, Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment, delayed allergy experiment, half hemolysis value (HC
50) mensuration and the mensuration of antibody-producting cell number; Immunity III group is carried out the inductive mouse lymphocyte transformation experiment of ConA, NK cell activity mensuration.
1.3 experimental situation condition: 23 ℃-26 ℃ of temperature, humidity 56%-68%, the experimental animal room quality certification number is No. 026.
1.4 dosage is selected and sample treatment:, be equivalent to every day 0.167mL/kgbw and establish the basic, normal, high dosage of health promoting wine of the present invention and be respectively 0.83mL/kgbw, 5.00mL/kgbw (be equivalent to respectively human body recommended dose 5,10,30 times) according to the oral recommended amounts of human body.Sample thief adding distil water preparation desired concn, matched group gives isopyknic distilled water, gives animal subject respectively and irritates stomach, irritates stomach every day once, irritates the long-pending 0.2mL/10gbw of being of body of stomach, continuous 30 days.
1.5 key instrument and reagent: animal table scale, analytical balance, clean bench, CO2 gas incubator, centrifuge, 722 spectrophotometers, constant water bath box, liquid glimmer instrument, microplate reader, microscope etc.
Aseptic operation apparatus, slide gauge, microsyringe, cell counter, the flat Tissue Culture Plate in 24 holes and 96 holes, 96 hole U type Tissue Culture Plates, glass dish, gauze, test tube, slide frame, 200 eye mesh screens, timer, hematochrome suction pipe, microscope slide etc.
Sheep red blood cell (SRBC) (SRBC), normal saline, Hank ' s liquid (PH7.2-7.4), the RPMI1640 culture fluid, calf serum, mycillin, ConA, 1% glacial acetic acid, the HCL solution of 1mol/L, acid isopropyl alcohol (the 96mL isopropyl alcohol adds the HCL solution 4mL of 1mol/L), MTT, PBS buffer (PH7.2-7.4), complement (guinea pig serum), the SA buffer, its agarose, Dou Shi reagent, the YAC-1 cell, lactic acid is received, the nitro tetrazolium chloride, azophenlyene dimethyl ester sulfate, oxidized form of nicotinamide-adenine dinucleotide, 0.2mol/L the Tris-HCL buffer, 1% NP40, india ink, 0.1% sodium carbonate, chicken red blood cell, methanol, Giemsa dye liquor etc.
1.6 experimental technique
1.6.1 internal organs/weight ratio pH-value determination pH: mice is put to death in the back of weighing, and takes out spleen and thymus, weighs on electronic analytical balance, calculates dirty/body ratio.
1.6.2 delayed allergy (DTH) (the sufficient sole of the foot thickens method)
After mouse peritoneal injection 2% (v/v) SRBC (the every Mus of 0.2M1/) sensitization 4 days, measure left back sufficient sole of the foot thickness,, measure left back sufficient sole of the foot portion thickness in injection back 24h then measuring point subcutaneous injection 20% (v/v) SRBC (the every Mus of 20ul/), same position is measured three times, averages.Metapedes sole of the foot thickness difference (swelling degree of the paw) was represented the degree of DTH in the past.
1.6.3ConA inductive mouse lymphocyte transformation experiment (mtt assay)
The aseptic spleen of getting places the little plate that fills an amount of aseptic Hank ' s liquid, makes cell suspension, filters through 200 eye mesh screens.Use Hank ' s liquid to wash 3 times, each centrifugal 10 minutes (1000rpm).Then with cell suspension in the 2mL complete culture solution, the living cell counting number, adjusting cell concentration with the RPMI1640 culture fluid is 5 * 10
6Individual/mL.Again cell suspension is divided two holes to add in 24 well culture plates, every hole 1mL, a hole adds 50uLConA liquid (being equivalent to 5ug/mL) therein, and 5% carbon dioxide is put in contrast in another hole, cultivates 72h for 37 ℃.Cultivate and finish preceding 4h, supernatant 0.7mL is inhaled in every hole gently, adds the RPMI1640 culture fluid that does not contain calf serum, add MTT (5mg/mL) 50Ul/ hole simultaneously, after continuing to finish, every hole adds 1mL acid isopropyl alcohol, the piping and druming mixing dissolves purple crystal fully.Then this liquid is measured wavelength 570nm with microplate reader.Lymphocytic multiplication capacity deducts the shading value that does not add the ConA hole with the optical density value that adds the ConA hole and represents.
1.6.4 antibody-producting cell detects (Jerne improves slide method)
Get Sanguis caprae seu ovis, with normal saline washing 3 times, centrifugal at every turn (200r/min) 10min is made into the cell suspension of 2% (v/v), every Mus lumbar injection 0.2mL with hematocrit SRBC with normal saline.Mice that immunity is back 4 days is put to death, and gets spleen, tears up gently, makes cell suspension with Hanks liquid, and 200 eye mesh screens filter, wash, centrifugal 2 times, at last with cell suspension in 5mL Hanks liquid.To mix with the PH7.4 of equivalent, the Hanks liquid of 2 times of concentration after the culture medium heating for dissolving of top layer, the packing small test tube, every pipe 0.5mL, in pipe, add 10%SRBC50ul (v/v)/20ul splenocyte suspension again with the preparation of SA liquid, be poured on the slide of brushing the thin layer agarose behind the mixing rapidly, treat after agarose solidifies the slide level to be buckled and be placed on the slide frame, put into CO2 gas incubator incubation 1.5h, complement (1: 10) with the dilution of SA liquid joins in the slide groove then, continues to count the hemolysis plaque number behind the incubation 1.5h.
1.6.5 half hemolysis value (HC
50) mensuration
Get Sanguis caprae seu ovis, with normal saline washing 3 times, every Mus carries out immunity through lumbar injection 2% (v/v prepares with normal saline) hematocrit RBC0.2mL.After 5 days, extract eyeball and get blood in centrifuge tube, place about 1h, solidification blood and tube wall are peeled off, serum is fully separated out, the centrifugal 10min of 2000rpm collects serum.Is 200 times with the SA buffer with the serum dilution, gets 1mL and puts in vitro, adds 10% (v/v is with the preparation of SA buffer) hematocrit SRBC0.5mL successively, complement 1mL (pressing dilution in 1: 10 with the SA buffer).Other establishes the not control tube of increase serum (replacing with the SA buffer).After putting in 37 ℃ of waters bath with thermostatic control insulation 30min, the ice bath cessation reaction.The centrifugal 10min of 2000rpm gets supernatant 1mL, adds Dou Shi reagent 3mL.(v/v with the preparation of SA buffer) the hematocrit SRBC0.25mL that gets 10% simultaneously, add Dou Shi reagent to 4mL in another test tube, abundant mixing, place 10min after, sentence control tube in 540nm and do blankly, measure respectively and respectively manage optical density value.The amount of hemolysin is with half hemolysis value (HC
50) expression, be calculated as follows: half hemolysis value (HC
50Optical density value * extension rate during)=sample optical density value/SRBC HD50
1.6.6 mice carbon is cleaned up experiment
Mouse tail vein injection is with the india ink of 4 times of normal saline dilutions, every 10g body weight injection 0.1mL, and timing immediately after prepared Chinese ink injects after injecting prepared Chinese ink the 2nd, 10min, from angular vein treating the preponderant disease instead of the secondary disease blood 20ul, joins 2mLNa respectively
2CO
3Shake up in the solution.With Na
2CO
3Solution is made blank, with 722 type spectrophotometers at 600nm wavelength place colorimetric photometry density value (OD).Mice is put to death, get liver, spleen, weigh, calculate phagocytic index a.
1.6.7 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment (half intracorporal method)
Chicken red blood cell (2000rpm, 10min) the suspension 1mL of mouse peritoneal injection 20% (v/v prepares with normal saline) hematocrit, interval 30min, the cervical vertebra dislocation is put to death, and gets peritoneal macrophage washing liquid 1mL, drip on microscope slide, put into the enamel box that is lined with wet gauze, put 37 ℃ of incubator incubation 30min.Incubate completely, rinsing in normal saline is to remove not paster cell.Dry, with methanol: acetone (1: 1) is fixing, the dyeing of 4% (v/v) Giemsa-phosphate buffer, and the rinsing of reuse distilled water is dried.The oil mirror is counting down, and 100 macrophages of every counting are calculated as follows phagocytic rate and phagocytic index:
Phagocytic rate %=engulfs macrophage number * 100 of the macrophage number/counting of chicken red blood cell
The macrophage number of chicken red blood cell sum/counting that phagocytic index=quilt is engulfed.
1.6.8NK the mensuration (isotope of cytoactive
3The H-TdR algoscopy)
Get the back well-grown YAC-1 cell of 24h (survival rate＞95%) that goes down to posterity by 1 * 10
6/ mLYAC-1 cell suspension adds
3H-TdR10 μ Ci carries out labelling, cultivates 2h in 37 ℃ of 5% CO2 gas incubator, every 30min vibration 1 time, and the cell behind the labelling is resuspended in the culture fluid with culture fluid washing 3 times, and making cell concentration is 1 * 10
5Individual/mL.Being tried the dislocation of mice cervical vertebra puts to death, the aseptic spleen of getting, make splenocyte suspension, use Hank ' s washing 3 times, each centrifugal 10min of 1000rpm, the RPMI1640 complete culture solution that reuse 2mL contains 10% calf serum suspends, and with the blue dyeing counting of platform phenol (viable count should more than 95%), adjusting cell concentration be 1 * 10
7Individual/mL.Every hole adds the target cell of 100uL labelling in 96 orifice plates, and test hole adds 100uL effector lymphocyte, and the blank hole adds the 100uL culture fluid, and maximum release aperture adds 100uLTriton X-100.Each sample is established 3 multiple holes, puts 5% carbon dioxide, cultivates 4h for 37 ℃.Get storage with the bull cell and get and combine on the glass fiber filter paper, measure per minute umber of pulse (cpm) with liquid scintillation instrument.
NK cytoactive=(1-experimental port cpm/ (the maximum release aperture cpm of blank hole cpm-)) * 100%
1.7 experimental data statistics: carry out statistical analysis with Excel/Spss software.
2 results
1.1 health promoting wine of the present invention is to the influence of mice body weight
By table 1-4 as seen, each dosage group is tested just, is tested mid-term, the last mice body weight of experiment and the contrast of experimental session weight of mice and compares there was no significant difference (P＞0.05).
Table 1 is respectively organized the initial body weight (x ± s) of mice
| The dosage group | Immunity I group | Immunity II group | Immunity III group | |||
| Number of animals (only) | Body weight (g) | Number of animals (only) | Body weight (g) | Number of animals (only) | Body weight (g) | |
| Contrast | ??10 | ??19.5±1.0 | ??10 | ??19.6±1.2 | ??10 | ?19.7±0.9 |
| Low | ??10 | ??19.5±1.0 | ??10 | ??19.6±1.2 | ??10 | ?19.7±0.9 |
| In | ??10 | ??19.5±0.9 | ??10 | ??19.5±1.0 | ??10 | ?19.6±0.9 |
| High | ??10 | ??19.6±1.0 | ??10 | ??19.6±1.1 | ??10 | ?19.7±1.0 |
Table 2 is respectively organized body weight in the mid-term (x ± s) of mice
| The dosage group | Immune group I group | Immunity II group | Immunity III group | |||
| Number of animals (only) | Body weight (g) | Number of animals (only) | Body weight (g) | Number of animals (only) | Body weight (g) | |
| Contrast | ??10 | 29.5±1.3 | ??10 | ?29.7±1.6 | ??10 | ?29.6±1.4 |
| Low | ??10 | 29.8±1.6 | ??10 | ?29.8±1.5 | ??10 | ?30.1±1.7 |
| In | ??10 | 29.7±1.3 | ??10 | ?29.0±1.5 | ??10 | ?29.8±1.4 |
| High | ??10 | 29.8±1.6 | ??10 | ?30.1±1.3 | ??10 | ?30.2±2.0 |
Table 3 is respectively organized the end body weight of mice
| The dosage group | Immunity I group | Immunity II group | Immunity III group | |||
| Number of animals (only) | Body weight (g) | Number of animals (only) | Body weight (g) | Number of animals (only) | Body weight (g) | |
| Contrast | ??10 | ?39.7±1.7 | ??10 | ?39.6±1.4 | ??10 | ?39.9±1.7 |
| Low | ??10 | ?39.8±2.0 | ??10 | ?39.5±2.1 | ??10 | ?40.1±2.5 |
| In | ??10 | ?39.3±1.4 | ??10 | ?39.4±1.9 | ??10 | ?39.5±1.9 |
| High | ??10 | ?39.4±1.9 | ??10 | ?39.9±2.4 | ??10 | ?39.6±2.1 |
Table 4 is respectively organized the body weight gain (x ± s) of mice
| The dosage group | Immunity I group | Immunity II group | Immunity III group | |||
| Number of animals (only) | Body weight gain (g) | Number of animals (only) | Body weight gain (g) | Number of animals (only) | Body weight gain (g) | |
| Contrast | ??10 | ?20.2±1.3 | ??10 | ?20.1±1.0 | ??10 | ?20.2±1.0 |
| Low | ??10 | ?20.3±1.2 | ??10 | ?19.9±1.6 | ??10 | ?20.5±1.9 |
| In | ??10 | ?19.8±1.2 | ??10 | ?19.9±1.2 | ??10 | ?19.9±1.4 |
| High | ??10 | ?19.8±1.4 | ??10 | ?20.4±1.6 | ??10 | ?19.8±1.5 |
2.2 health promoting wine of the present invention is to the influence of mouse immune organ internal organs/body weight ratio
Table 5 health promoting wine of the present invention is to the influence of mouse immune organ internal organs/body weight ratio
| The dosage group | Number of animals (only) | Spleen/body weight | Thymus/body weight | ||
| ???(％) | The P value | ????(％) | The P value | ||
| Contrast | ??10 | ???0.54±0.05 | ????0.37±0.04 | ||
| Low | ??10 | ???0.53±0.04 | ????0.814 | ????0.38±0.05 | ??0.949 |
| In | ??10 | ???0.53±0.04 | ????0.729 | ????0.36±0.04 | ??0.732 |
| High | ??10 | ???0.54±0.05 | ????0.970 | ????0.38±0.03 | ??0.960 |
By table 5 as seen, per os gives the health promoting wine 30 days of mice various dose, and spleen/body weight and the thymus/body weight ratio of mice is not made significant difference.
2.3 health promoting wine of the present invention is to the mouse cell Immune Effects
2.3.1 health promoting wine of the present invention is to the influence of mice delayed allergy (DTH)
Table 6 health promoting wine of the present invention is to the influence of mice delayed allergy (DTH) (x ± s)
| The dosage group | Number of animals (only) | Injection back 2h swelling degree of the paw (mm) | The P value |
| Contrast | ??10 | ??0.396±0.087 | ??--- |
| Low | ??10 | ??0.494±0.121 | ??0.160 |
| In | ??10 | ??0.534±0.118 | ??0.030 * |
| High | ??10 | ??0.565±0.132 | ??0.007 * |
As shown in Table 6, per os gives the health promoting wine 30 days of mice various dose, and dosage group mice swelling degree of the paw and matched group relatively have the trend of increasing, middle and high dosage group and matched group relatively, difference has significance.
2.3.2 health promoting wine of the present invention is to the influence of the inductive mouse lymphocyte conversion capability experiment of mice ConA
By table 7 as seen, per os gives the health promoting wine 30 days of mice various dose, and each dosage group is not seen obvious influence to the mouse lymphocyte conversion capability.
The influence that table 7 health promoting wine of the present invention is tested the mouse lymphocyte conversion capability (x ± s)
| The dosage group | Number of animals (only) | Lymphopoiesis ability (OD difference) | The P value |
| Contrast | ??10 | ????0.063±0.038 | ??---- |
| Low | ??10 | ????0.081±0.035 | ??0.681 |
| In | ??10 | ????0.092±0.050 | ??0.308 |
| High | ??10 | ????0.083±0.043 | ??0.608 |
2.4 health promoting wine of the present invention is to the influence of humoral immunization
2.4.1 health promoting wine of the present invention is to the influence of mouse antibodies cellulation number
Table 8 health promoting wine of the present invention is to the influence of mouse antibodies cellulation number
| The dosage group | Number of animals (only) | Hemolysis plaque number (* 10 3/ full spleen) | The P value |
| Contrast | ??10 | ????18.93±7.62 | ??---- |
| Low | ??10 | ????22.53±7.68 | ??0.625 |
| In | ??10 | ????23.70±8.29 | ??0.411 |
| High | ??10 | ????29.13±8.40 | ??0.019 |
As shown in Table 8, per os gives the health promoting wine 30 days of mice various dose, and dosage group mouse antibodies cellulation number and matched group relatively have the trend of increasing, high dose group and matched group relatively, difference has significance.
2.4.2 health promoting wine of the present invention is to mice half hemolysis value (HC
50) influence
Table 9 health promoting wine of the present invention is to mice half hemolysis value (HC
50) influence (x ± s)
| The dosage group | Number of animals (only) half hemolysis value | The P value | |
| Contrast | ??10 | ????181.9±45.1 | ??--- |
| Low | ??10 | ????222.9±49.1 | ??0.200 |
| In | ??10 | ????254.1±52.3 | ??0.010 |
| High | ??10 | ????255.2±58.9 | ??0.008 |
By table 9 as seen, per os gives the health promoting wine 30 days of mice various dose, and dosage group mice half hemolysis value and matched group relatively have the trend of increasing, middle and high dosage group and matched group relatively, difference has significance.
2.5 health promoting wine of the present invention is to the influence of mouse monokaryon one macrophage phagocytic function
2.5.1 the influence that health promoting wine of the present invention is cleaned up mouse monokaryon one macrophage carbon
The influence that table 10 health promoting wine of the present invention is cleaned up mouse monokaryon one macrophage carbon (x ± s)
| The dosage group | Number of animals (only) | Phagocytic index (a) | The P value |
| Contrast | ??10 | ????5.42±1.48 | |
| Low | ??10 | ????5.99±0.95 | ??0.562 |
| In | ??10 | ????7.10±1.02 | ??0.007 |
| High | ??10 | ????7.40±1.06 | ??0.001 |
By table 10 as seen, per os gives the health promoting wine 30 days of mice various dose, and dosage group mice phagocytic index and matched group relatively have the middle and high dosage group of the trend of increasing mice phagocytic index to be significantly higher than matched group, and difference has significance.
2.5.2 health promoting wine is engulfed the influence of chicken red blood cell function to mouse macrophage
Table 11-1 health promoting wine is engulfed the influence (x ± s) of chicken red blood cell phagocytic rate to mouse macrophage
| The dosage group | Number of animals (only) | Phagocytic rate (%) | Phagocytic rate square root arcsine conversion value | The P value |
| Contrast | ??10 | ??28.4±6.5 | ????32.1±4.1 | |
| Low | ??10 | ??29.8±6.2 | ????33.0±3.9 | ????0.895 |
| In | ??10 | ??31.3±4.6 | ????34.0±2.9 | ????0.503 |
| High | ??10 | ??31.0±5.1 | ????33.8±3.2 | ????0.591 |
Table 11-2 health promoting wine is engulfed the influence (x ± s) of chicken red blood cell phagocytic index to mouse macrophage
| The dosage group | Number of animals (only) | Phagocytic index | The P value |
| Contrast | ??10 | ????0.844±0.275 | |
| Low | ??10 | ????0.923±0.278 | ??0.846 |
| In | ??10 | ????1.087±0.261 | ??0.120 |
| High | ??10 | ????0.998±0.248 | ??0.435 |
By table 11 as seen, per os gives the health promoting wine 30 of mice various dose, and each dosage group is engulfed the chicken red blood cell ability to mouse macrophage and do not seen obvious influence.
2.6 health promoting wine of the present invention is to the active influence of NK cells in mice
Table 12 health promoting wine is to the active influence of NK cells in mice
| The dosage group | Number of animals (only) | NK cytoactive (%) | NK cytoactive square root arcsine conversion value | The IP value |
| Contrast | ??10 | ??36.9±6.0 | ??37.3±3.6 | |
| Low | ??10 | ??38.8±6.4 | ??38.5±3.7 | ??0.833 |
| In | ??10 | ??40.1±6.2 | ??39.2±3.6 | ??0.560 |
| High | ??10 | ??39.6±7.0 | ??38.9±4.1 | ??0.681 |
By table 12 as seen, per os gives the health promoting wine 30 days of mice various dose, and each dosage group is not seen obvious influence to the NK cells in mice activity.
3 conclusions
Per os gives the health promoting wine 30 days of mice 0.83mL/kgbw, 1.67mL/kgbw, 5.00mL/kgbw dosage, can improve the ability that delayed allergy, half hemolysis value, antibody-producting cell number, the monokaryon one macrophage carbon of mice are cleaned up.Weight of mice, thymus body weight ratio, spleen body weight ratio, the inductive mouse lymphocyte conversion capability of ConA, mouse macrophage are engulfed ability, the NK cell activity of chicken red blood cell and all do not had obvious influence.Point out this sample to have the enhancing immunity function.
Health promoting wine of the present invention has the characteristics of safety non-toxic.
Health promoting wine of the present invention carries out the safety toxicological evaluation by " health food check and assessment technique standard " (version in 2003), and the result is as follows:
1 acute oral LD
50Result: to acute oral LD female, male mice
50All, belong to non-poisonous material greater than 21500mg/kgbw.
2 three genetic toxicity test results: the result is all negative for three genetic toxicity tests (Salmonella reversion test, PCEMNR micronucleus test, mouse sperm deformity test).
Fed the result in 3 30 days: the health promoting wine with 4.2mL/kgbw, 8.3mL/kgbw, 16.7mL/kgbw dosage is given rat oral gavage 30 days, duration of test, and each dosage group rat body weight growth, affairs utilization rate and matched group be there was no significant difference (P＞0.05) relatively.Female, male Mus blood biochemistry index, routine blood test index and liver/body, spleen/body, kidney/body, male Mus testis/body ratio and compare difference there are no significant (P＞0.05).Liver,spleen,kidney, stomach, duodenum, testis (ovary) tissue pathology checking do not have obvious damage.The prompting submitted sample was fed the every observation index of rat is not produced obvious toxic-side effects in 30 days.
The specific embodiment
Further explain the present invention in the mode of embodiment below, but the present invention is not limited to embodiment.
Embodiment 1
(1) 10kg Radix Panacis Quinquefolii and 5kg are pulverized Radix Ophiopogonis, crossed 20 purpose sieves;
(2) mass percent concentration that adds 200kg at Radix Panacis Quinquefolii with in Radix Ophiopogonis is 50% ethanol, under 95 ℃ temperature, refluxed 2 hours, filter, obtain extracting solution with the rotating speed of 400 purpose tripodia frame filter centrifugals with 3000rpm, repeat again to extract and filter merge extractive liquid, 2 times;
(3) extracting solution that merges is evaporated to relative density when being 1.35 (50 ℃), obtains extractum;
(4) white spirit mixed of the extractum that step (3) is obtained, 30kg chitin and 483kg preparation obtains described health promoting wine.Chinese liquor is the aromatic Chinese spirit of liquor-saturated board 36 degree in the Kowloon of Kowloon, Chengde Industry Co.,Ltd.Chitin is the triumphant sharp biological preparation products factory product in Guangzhou.
Embodiment 2
(1) 8kg Radix Panacis Quinquefolii and 7kg are pulverized Radix Ophiopogonis, crossed 20 purpose sieves;
(2) mass percent concentration that adds 180kg at Radix Panacis Quinquefolii with in Radix Ophiopogonis is 50% ethanol, under 95 ℃ temperature, refluxed 2 hours, filter, obtain extracting solution with the rotating speed of 400 purpose tripodia frame filter centrifugals with 3000rpm, repeat again to extract and filter merge extractive liquid, 2 times;
(3) extracting solution that merges is evaporated to relative density when being 1.35 (50 ℃), obtains extractum;
(4) white spirit mixed of the extractum that step (3) is obtained, 35kg chitin and 540kg preparation obtains described health promoting wine.Chinese liquor is the aromatic Chinese spirit of liquor-saturated board 38 degree in the Kowloon of Kowloon, Chengde Industry Co.,Ltd.Chitin is the triumphant sharp biological preparation products factory product in Guangzhou.
Embodiment 3
(1) 12kg Radix Panacis Quinquefolii and 4kg are pulverized Radix Ophiopogonis, crossed 20 purpose sieves;
(2) mass percent concentration that adds 220kg at Radix Panacis Quinquefolii with in Radix Ophiopogonis is 50% ethanol, under 95 ℃ temperature, refluxed 1.5 hours, filter, obtain extracting solution with the rotating speed of 400 purpose tripodia frame filter centrifugals with 3000rpm, repeat again to extract and filter merge extractive liquid, 2 times;
(3) extracting solution that merges is evaporated to relative density when being 1.35 (50 ℃), obtains extractum;
(4) white spirit mixed of the extractum that step (3) is obtained, 30kg chitin and 470kg preparation obtains described health promoting wine.Chinese liquor is the aromatic Chinese spirit of liquor-saturated board 36 degree in the Kowloon of Kowloon, Chengde Industry Co.,Ltd.Chitin is the triumphant sharp biological preparation products factory product in Guangzhou.
Claims (4)
1. the health promoting wine of an enhancing immunity is characterized in that it is made by the raw material of following weight portion: 8～12 parts of Radix Panacis Quinquefoliis, 25～35 parts of chitins, 3～7 parts of Radix Ophiopogonis, 450～550 parts of Chinese liquor.
2. health promoting wine according to claim 1 is characterized in that it is made by the raw material of following weight portion: 10 parts of Radix Panacis Quinquefoliis, 30 parts of chitins, 5 parts of Radix Ophiopogonis, 480 parts of Chinese liquor.
3. health promoting wine according to claim 1 and 2, the number of degrees that it is characterized in that described Chinese liquor are 30～60.
4. the preparation method of the described health promoting wine of claim 1 is characterized in that it may further comprise the steps:
(1) Radix Panacis Quinquefolii and Radix Ophiopogonis are pulverized, crossed 20 purpose sieves;
(2) add at Radix Panacis Quinquefolii with in Radix Ophiopogonis Radix Panacis Quinquefolii and Radix Ophiopogonis the weight sum the mass percent concentration of 4～6 times of weight be 50% ethanol, under 95 ℃ temperature, refluxed 1～3 hour, filter with the rotating speed of 400 purpose filters with 3000rpm, obtain extracting solution, repeat again to extract and filter merge extractive liquid, 2 times;
(3) extracting solution that merges being evaporated to relative density is 1.35 o'clock, obtains extractum;
(4) with extractum, chitin and white spirit mixed preparation, obtain described health promoting wine.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103666919A (en) * | 2013-12-03 | 2014-03-26 | 祝迈 | American ginseng healthcare red wine |
| CN105695269A (en) * | 2016-03-25 | 2016-06-22 | 安徽瑞思威尔科技有限公司 | Sophora flower bud wine and preparation method |
| CN106318780A (en) * | 2016-10-17 | 2017-01-11 | 上海宇元生物科技有限公司 | Production method of chitosan bamboo tube wine |
| CN106479804A (en) * | 2016-10-17 | 2017-03-08 | 上海宇元生物科技有限公司 | The preparation method of chitin red pitaya wine |
| CN106754123A (en) * | 2016-12-01 | 2017-05-31 | 威海新异生物科技有限公司 | A kind of American ginseng wine preparation method |
| CN107648485A (en) * | 2017-08-22 | 2018-02-02 | 安龙县鹿宝酒坊 | A kind of healthy medicated wine of strengthen immunity and preparation method thereof |
-
2004
- 2004-09-30 CN CN 200410080510 patent/CN1252241C/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103666919A (en) * | 2013-12-03 | 2014-03-26 | 祝迈 | American ginseng healthcare red wine |
| CN105695269A (en) * | 2016-03-25 | 2016-06-22 | 安徽瑞思威尔科技有限公司 | Sophora flower bud wine and preparation method |
| CN106318780A (en) * | 2016-10-17 | 2017-01-11 | 上海宇元生物科技有限公司 | Production method of chitosan bamboo tube wine |
| CN106479804A (en) * | 2016-10-17 | 2017-03-08 | 上海宇元生物科技有限公司 | The preparation method of chitin red pitaya wine |
| CN106754123A (en) * | 2016-12-01 | 2017-05-31 | 威海新异生物科技有限公司 | A kind of American ginseng wine preparation method |
| CN107648485A (en) * | 2017-08-22 | 2018-02-02 | 安龙县鹿宝酒坊 | A kind of healthy medicated wine of strengthen immunity and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1252241C (en) | 2006-04-19 |
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