CN1597569A - Culturing method of microbe for high temperature waste water biochemical treatment - Google Patents
Culturing method of microbe for high temperature waste water biochemical treatment Download PDFInfo
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- CN1597569A CN1597569A CN 03133995 CN03133995A CN1597569A CN 1597569 A CN1597569 A CN 1597569A CN 03133995 CN03133995 CN 03133995 CN 03133995 A CN03133995 A CN 03133995A CN 1597569 A CN1597569 A CN 1597569A
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Abstract
A process for culturing the microbes used to biochemically treat high-temp sewage includes such steps as choosing the active sludge in ordinary biochemical aeratnig pool as bacterial seeds, depositing, aerating, adding the sewage of oil field, removing supernatant, adding stabilizer of protein and growth promoter of microbes, and naturolizing.
Description
Technical field
The present invention relates to a kind of method for culturing microbes, specifically, is a kind of microbial culture method that is used for decomposing the hot wastewater organic pollutant, especially handles the method for culturing microbes that the high temperature oil extraction waste water is used.
Background technology
Microbial method dispose of sewage have that treatment capacity is big, purification rate is high, energy consumption is low, advantage such as simple to operate, non-secondary pollution, obtained to use widely at sewage treatment area.But common microorganism can only be applicable to the processing of normal temperature or middle temperature (general below 35 ℃) waste water, and common micro-organisms can not be survived under hot conditions, can't reach the purpose of purifying waste water certainly.
In some industrial production, can give off the higher waste water of temperature, but, can not carry out heat recuperation owing to reasons such as energy grade are low.For example the wastewater temperature of discharging in the oil recovery process is usually between 38～65 ℃, and contains many oil recovery auxiliary agents, according to traditional microbial treatment method, at first needs oil extraction waste water is cooled to then enter biological treatment system below 35 ℃.But the cooling apparatus investment is big, perishable, needs often maintenance, also contains the chemical assistant that more salt and oil recovery process add simultaneously in the oil extraction waste water usually, and these all are to using microbial method to handle the disadvantage of oil extraction waste water.Oil field extracted water mainly adopts methods such as oil removal, coarse, coagulation, filtration, sterilization or air supporting to remove impurity such as petroleum-type, suspended substance, iron and bacterium at present; but these technologies can be removed suspended substance and part petroleum substance; indexs such as the COD of water outlet are still higher, do not meet the requirement of more and more stricter environmental law rules.
The general acclimation method of active sludge generally has two kinds at present: 1. throw excrement multiplication activity mud, breed when being scheduled to settling ratio, add the actual waste water blast aeration, by taming step by step to full dose on a small quantity.2. at first screen the strong microorganism strains of capacity of decomposition, put small incubation case hyperalimentation fluid breeding into after, the settling ratio that reaches required is bred in the pond that has high input again.In the prior art, once there was patent report to cross several method for culturing microbes, as described in CN1068088A and CN1144535A, wherein CN1068088A discloses a kind of method of synchronized culture active sludge, though this patented technology can be tamed the source science and engineering active sludge of sewage already by synchronized culture, but it still belongs to the normal temperature active sludge, inapplicable hot wastewater.CN1144535A discloses the method for culturing micro-organisms, by the cultivation of microorganism, sudden change being handled and stabilization contains in the sudden change fermenting container of matrix of nutritious material one simultaneously and carries out, but fast culture goes out to be used for the microorganism of decomposing harmful substances, but method is loaded down with trivial details, be difficult for grasp.
Summary of the invention
At the deficiencies in the prior art, the invention provides and a kind ofly use normal temperature active sludge in the common biochemical aeration tank as original strain, cultivate the high temperature microbe of handling hot wastewater.Utilize the high temperature microbe of being cultivated directly to handle oil extraction waste water, not only saved refrigerative link and expense, from the angle of biochemical kinetics, the raising of temperature helps improving biochemical reaction rate, thereby accelerate wastewater treatment speed, shorten hydraulic detention time, reduce facility investment.
The culturing process that the present invention handles the hot wastewater microorganism is:
Active sludge in the common biochemical aeration tank after filtration, sedimentation, abandoning supernatant, add waste water below 35 ℃, add nutritive substance, protein thermo-stabilizer and microorganism growth-promoting agent simultaneously, carried out aeration 0.5～3 day, preferred 1～2 day, discharge supernatant liquor after the sedimentation.Tame according to the operation scheme of intermittent activated sludge process then, adopt the mode move 1～2 cycle every day, heat up by 0.5 ℃～3 ℃/day amplitude simultaneously, preferably heat up by 1～2 ℃/day amplitude.The settling ratio of active sludge reaches 20%～30% in biochemical reaction tank, and the clearance of COD reaches emission standard, and when temperature of reaction rose to 40～65 ℃, domestication finished.
Wherein nutritive substance is nitrogen and phosphorus, and the protein thermo-stabilizer is a glucose.
Wherein the microorganism growth-promoting agent comprises organic acid, biological buffer, nutritive substance, it is formed weight ratio and presses organic acid: biological buffer: nutritive substance is (10～50): (1～10): (50～100), preferably also comprise amino acid and/or trace element etc. in the microorganism growth-promoting agent, its weight ratio is pressed organic acid: biological buffer: nutritive substance: amino acid: trace element is (10～50): (1～10): (50～100): (10～20): (1～5).
Organic acid described in the microorganism growth-promoting agent is one or more in the ketone acid of the alcohol acid of the carboxylic acid of 1～6 carbon, 2～6 carbon and 3～6 carbon, and wherein ketone acid is preferably pyruvic acid.Described amino acid is D-amino acid, as in D-Serine, D-L-Ala or the D-halfcystine one or more.Described biological buffer is a thionamic acid type biological buffer, as 2-(N-sign indicating number quinoline) ethyl sulfonic acid, N, N-two (2-hydroxyethyl)-2-amido ethyl sulfonic acid, N, one or more in N '-two (2-ethanesulfonic acid) piperazine.Described nutritive substance is a carbohydrate, and carbohydrate can be low-carbohydrate and/or polyose, and low-carbohydrate is one or more in sucrose, lactose, the raffinose, and polyose is one or more in natural gum, pectin, the agar.Described trace element is K
+, Mn
2+, Mg
2+, Fe
3+, Ca
2+In one or more, preferred Mn
2+, Mg
2+, Fe
3+In one or more, trace element can derive from its soluble inorganic salt, as nitrate, vitriol, muriate etc., if select several trace elements, its weight ratio is: K
+: Mn
2+: Mg
2+: Fe
3+: Ca
2+=(2～10): (2～6): (1～4): (1～2): (15～50).
Nutritive substance is in COD: N: P=200 between domestication: the ratio of 5～10: 1～2 (weight ratio) adds urea and Sodium phosphate dibasic nitrogenous source and the phosphorus source as the microorganism growth breeding; In glucose performance COD and waste water COD is 0.2～5: 1 ratio adding glucose solution, preferred 1～2: 1; Concentration by 1～20mg/L adds the microorganism growth-promoting agent, preferred 3～10mg/L.Aeration operation required time ratio is big slightly during domestication, can account for 75～95% of each cycle, and the air water volume ratio is generally 70～120, and volumetric loading is 0.1～1.0kgCOD/m
3D.
Adopt the active sludge in the common biochemical aeration tank,, add oil extraction waste water and intermittently cultivate, discharge supernatant liquor, add protein stabilizing agent and microorganism growth-promoting agent in proportion, cultivate the microorganism that hot wastewater is handled in domestication through the precipitation aeration.The high temperature microbe that obtains can be handled oil extraction waste water effectively.Whole process repeats in a batch reactor.It is simple to have method, and cost is low, the characteristics that the domestication time is short.The domestication beginning is at first carried out the long period aeration with oil extraction waste water, can screen the adaptable bacterial classification of oil extraction waste water group.The microorganism growth-promoting agent that adds in the domestication process is at first alleviated the toxicant that exists in the oil extraction waste water, for microorganism is wherein created good living environment, wherein contained enzyme also plays a role immediately, some larger molecular organicses are degraded, make it to become the small organic molecule that more helps microbiological deterioration.Therefore, add the microorganism growth-promoting agent and have vital role for successfully cultivating the microorganism that is applicable to hot environment and shortening acclimation period.
Embodiment
The concrete culturing process that the present invention handles the hot wastewater microorganism is:
1. the collection of bacterial classification is gathered active sludge from common biochemical aeration tank, after filtration, sedimentation, abandoning supernatant, isolates purified active sludge, and active sludge requires fresh, and color shows tawny.
2. the cultivation of high temperature microbe pumps into one intermittently in the biochemical reaction tank with oil extraction waste water, adds nutritive substance, protein thermo-stabilizer and microorganism growth-promoting agent in proportion, and supernatant liquor is discharged in blast aeration 1～3 day after the sedimentation.After this adopt the mode of moving for 1～2 cycle every day to move.Going round and beginning again, the active sludge settling ratio reaches 20%～30% in reaction tank.
Because of adopting active sludge in the common biochemical aeration tank as bacterial classification, its adaptive temperature is generally between 15 ℃～35 ℃, be not suitable for being rapidly heated, so present method adopts the method that progressively heats up to cultivate high temperature microbe, the intensification amplitude is 1 ℃～3 ℃/day, the intensification amplitude is littler during beginning, so that bacterial classification adapts to new environment gradually.Along with the continuous rising of temperature, unconformable microbial death is constantly arranged and disintegrate, discharge reaction tank along with water outlet.The active sludge that retains in the reaction tank has then adapted to hot environment gradually.Cultivate the domestication later stage, the settling property of active sludge strengthens gradually.Survey settling ratio half an hour, water outlet COD every day, irregularly carry out microscopy.Keep the interior dissolved oxygen of biochemical reactor greater than 0.5mg/L.Along with the carrying out of domestication process, the increase of high temperature microbe flco can reduce microorganism growth-promoting agent, nutrition, the protein stabilizing agent ratio in waste water gradually.
3. tame the sign that finishes: the settling ratio of active sludge reaches 20%～30% in biochemical reaction tank, and when the clearance of COD reaches emission standard, can think that domestication work finishes, this moment, the microorganism flco was faint yellow, and this active sludge can formally be handled the high temperature oil extraction waste water.
4. handle waste water: the concentration of cultured high temperature microbe by 2000～4000mg/L is added in the biochemical reaction tank at intermittence, directly introduce high temperature (40～65 ℃) oil extraction waste water and handle, utilize the life Metabolic activity of microorganism to decompose organic pollutant in the oil extraction waste water.Waste water is purified, and then reaches discharging standards.
Further specify the present invention program and effect below by example.
Each embodiment microorganism growth-promoting agent of table 1 the inventive method is formed
| Form | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Organic acid | Pyruvic acid | Pyruvic acid: butyric acid=2: 1 | Levulinic acid: lactic acid=1: 3 |
| Biological buffer | 2-(N-sign indicating number quinoline) ethyl sulfonic acid | 2-(N-sign indicating number quinoline) ethyl sulfonic acid: N, N ' two (2-ethanesulfonic acid) piperazine=3: 1 | N, N-two (2-hydroxyethyl)-2-amido ethyl sulfonic acid |
| Nutritive substance | Agar | Agar: sucrose=1: 1 | Pectin: lactose=1: 3 |
| Amino acid | D-Serine: D-L-Ala=4: 1 | ??/ | The D-Serine |
| Trace element | ????Mn 2+∶Mg 2+∶Fe 3+＝5 ????∶3∶1 | ??/ | ??K +∶Mn 2+∶Mg 2+∶Fe 3+∶ ??Ca 2+＝3∶5∶2∶1∶30 |
| Weight proportion | ????20∶8∶80∶15∶4 | ??40∶2∶100 | ??15∶5∶50∶20∶2 |
Each material consumption is a part by weight in the table.
Embodiment 1
A kind of land oil extraction waste water, COD are 656mg/L, and temperature is 55 ℃.
The collection of bacterial classification is gathered active sludge from common biochemical aeration tank, after filtration, sedimentation, abandoning supernatant, isolates purified active sludge, and active sludge is fresh, and color is a tawny.
Adding temperature and be 25 ℃, COD content in activity is dirty is the oil extraction waste water of 656mg/L, and by COD: N: P=200: the ratio of 5: 1 (weight ratio) adds nitrogenous source and the phosphorus source that urea and Sodium phosphate dibasic are bred as microorganism growth; It in glucose performance COD and waste water COD 1: 1 ratio adding glucose solution; Concentration by 4mg/L adds the microorganism growth-promoting agent.At the control dissolved oxygen is that 0.6mg/L carried out aeration 2 days, discharges supernatant liquor after the sedimentation.After this adopt the mode of moving 1 cycle every day to move, the aeration operation is 20 hours, and the water inlet operation is 1 hour, and the sedimentation operation is 2 hours, and drainage procedure is 1 hour, and the aeration air is 90 with the wastewater volume ratio, and volumetric loading is 0.42kgCOD/m
3D.Domestication was carried out 20 days, and temperature raising every day is 1 ℃ in preceding 10 days, and temperature raising every day is 2 ℃ in back 10 days.Preceding 15 days is 1.5: 1 ratio adding glucose solution in glucose performance COD and waste water COD, adds the microorganism growth-promoting agent by the concentration of 6mg/L, and add-on reduced by half in back 5 days.After the said process domestication, the active sludge settling ratio reaches 25% in the reaction tank, the reaction tank temperature reaches 55 ℃, the COD clearance reaches 85%, microorganism has had the adaptability of handling this oil extraction waste water, can think that domestication finishes, and can handle this waste water by intermittent activated sludge process, the adaptability of microorganism can further strengthen in steady running, and the clearance of COD will further improve.
Embodiment 2
A kind of marine oil field oil extraction waste water, COD are 493mg/L, and temperature is 58 ℃.
The collection of bacterial classification is gathered active sludge from common biochemical aeration tank, after filtration, sedimentation, abandoning supernatant, isolates purified active sludge, and active sludge is fresh, and color is a tawny.
Adding temperature and be 23 ℃, COD content in activity is dirty is the oil extraction waste water of 493mg/L, and by COD: N: P=200: the ratio of 10: 1 (weight ratio) adds nitrogenous source and the phosphorus source that urea and Sodium phosphate dibasic are bred as microorganism growth; It in glucose performance COD and waste water COD 2: 1 ratio adding glucose solution; Concentration by 8mg/L adds the microorganism growth-promoting agent.At the control dissolved oxygen is that 0.6mg/L carried out aeration 3 days, discharges supernatant liquor after the sedimentation.After this adopt the mode of moving 1 cycle every day to move, the aeration operation is 20 hours, and the water inlet operation is 1 hour, and the sedimentation operation is 2 hours, and drainage procedure is 1 hour, and the aeration air is 90 with the wastewater volume ratio, and volumetric loading is 0.35kgCOD/m
3D.Domestication was carried out 20 days, and temperature raising every day is 1.5 ℃ in preceding 10 days, and temperature raising every day is 2 ℃ in back 10 days.Preceding 10 days is 3.5: 1 ratio adding glucose solution in glucose performance COD and waste water COD, adds the microorganism growth-promoting agent by the concentration of 10mg/L, back 10 days add-ons measure for this reason 20%.After the said process domestication, the active sludge settling ratio reaches 20% in the reaction tank, the reaction tank temperature reaches 58 ℃, the COD clearance reaches 80%, microorganism has had the adaptability of handling this oil extraction waste water, can think that domestication finishes, and can handle this waste water by intermittent activated sludge process, the adaptability of microorganism can further strengthen in steady running, and the clearance of COD will further improve.
Embodiment 3
A kind of refinery waste discharge, the temperature of waste water are 45 ℃, and the COD concentration of waste water is about 1000mg/L.
The collection of bacterial classification is gathered active sludge from common biochemical aeration tank, after filtration, sedimentation, abandoning supernatant, isolates purified active sludge, and active sludge is fresh, and color is a tawny.
Adding temperature and be 22 ℃, COD content in activity is dirty is the refinery water of 1000mg/L, and by COD: N: P=200: the ratio of 6: 1 (weight ratio) adds nitrogenous source and the phosphorus source that urea and Sodium phosphate dibasic are bred as microorganism growth; It in glucose performance COD and waste water COD 4: 1 ratio adding glucose solution; Concentration by 8mg/L adds the microorganism growth-promoting agent.At the control dissolved oxygen is to carry out aeration more than the 0.6mg/L 18 hours, discharges supernatant liquor after the sedimentation.Employing moves the mode in 1 cycle every day and moves 10 days, and heat up 1 ℃ preceding 3 day every day, heats up 2 ℃ then every day, the aeration operation is 20 hours, and the water inlet operation is 1 hour, and the sedimentation operation is 2 hours, drainage procedure is 1 hour, and the aeration air is 90 with the wastewater volume ratio, and volumetric loading is 0.48kgCOD/m
3D.Move 6 days by the mode of moving 2 cycles every day then, heat up 1 ℃ every day, each activity time reduces by half by aforesaid operations, and volumetric loading is 0.96kgCOD/m
3D.Preceding 10 days is 2: 1 ratio adding glucose solution in glucose performance COD and waste water COD, adds the microorganism growth-promoting agent by the concentration of 7mg/L, back 5 days add-ons measure for this reason 30%.After the said process domestication, the active sludge settling ratio reaches 33% in the reaction tank, the reaction tank temperature reaches 45 ℃, the COD clearance reaches 85%, microorganism has had the adaptability of handling this refinery water, can think that domestication finishes, and can handle this waste water by intermittent activated sludge process, the adaptability of microorganism can further strengthen in steady running, and the clearance of COD will further improve.
Claims (10)
1, a kind of processing hot wastewater method for culturing microbes, it is characterized in that with the active sludge in the common biochemical aeration tank after filtration, sedimentation, abandoning supernatant, add waste water below 35 ℃, add nutritive substance, protein thermo-stabilizer and microorganism growth-promoting agent simultaneously, carry out blast aeration 0.5～3 day, and discharged supernatant liquor after the sedimentation; Tame according to the operation scheme of intermittent activated sludge process then, adopt the mode move 1～2 cycle every day, heat up by 0.5 ℃～3 ℃/day amplitude simultaneously; The settling ratio of active sludge reaches 20%～30% in biochemical reaction tank, and the clearance of COD reaches emission standard, and when temperature of reaction rose to 40～65 ℃, domestication finished.
2, according to the described cultural method of claim 1, it is characterized in that described nutritive substance is nitrogen and phosphorus, the protein thermo-stabilizer is a glucose, the microorganism growth-promoting agent comprises organic acid, biological buffer, nutritive substance, and the composition of microorganism growth-promoting agent is 10～50: 1～10: 50～100 by weight.
3, according to the described cultural method of claim 2, it is characterized in that also comprising amino acid and/or trace element in the described microorganism growth-promoting agent, by weight organic acid: biological buffer: nutritive substance: amino acid: trace element is 10～50: 1～10: 50～100: 10～20: 1～5.
4, according to claim 2 or 3 described cultural methods, it is characterized in that described organic acid is one or more in the alcohol acid of the carboxylic acid of 1～6 carbon, 2～6 carbon and the ketone acid with 3～6 carbon, described amino acid is D-amino acid, described biological buffer is a thionamic acid type biological buffer, and described nutritive substance is a carbohydrate.
5, according to the described cultural method of claim 4, it is characterized in that described organic acid is a pyruvic acid, described amino acid is one or more in D-Serine, D-L-Ala or the D-halfcystine, described biological buffer is 2-(N-sign indicating number quinoline) ethyl sulfonic acid, N, N-two (2-hydroxyethyl)-2-amido ethyl sulfonic acid, N, in N '-two (2-ethanesulfonic acid) piperazine one or more, described carbohydrate is low-carbohydrate and/or polyose, described trace element is K
+, Mn
2+, Mg
2+, Fe
3+, Ca
2+In one or more.
6, according to the described cultural method of claim 5, it is characterized in that described low-carbohydrate is one or more in sucrose, lactose, the raffinose, polyose is one or more in natural gum, pectin, the agar, and described trace element derives from its soluble inorganic salt.
7,, it is characterized in that its weight ratio of described trace element is: K according to the described cultural method of claim 5
+: Mn
2+: Mg
2+: Fe
3+: Ca
2+=2～10: 2～6: 1～4: 1～2: 15～50.
8, according to claim 1 or 2 described cultural methods, it is characterized in that described nutritive substance additional proportion is by weight COD: N: P=200: 5～10: 1～2 ratio adding urea and Sodium phosphate dibasic are as the nitrogenous source and the phosphorus source of microorganism growth breeding; It in glucose performance COD and waste water COD 0.2～5: 1 ratio adding glucose solution; Concentration by 1～20mg/L adds the microorganism growth-promoting agent.
9,, it is characterized in that in glucose performance COD and waste water COD being 1～2: 1 ratio adding glucose solution according to the described cultural method of claim 8; Concentration by 3～10mg/L adds the microorganism growth-promoting agent.
10, according to the described cultural method of claim 1, it is characterized in that described increase along with the high temperature microbe flco, can reduce nutrition, protein stabilizing agent, the ratio of microorganism growth-promoting agent in waste water gradually.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104891648A (en) * | 2015-07-07 | 2015-09-09 | 成都营星科技有限公司 | Cultivation method of active sludge for treating three-compound combination flooding oily sewage |
| CN111362421A (en) * | 2019-12-30 | 2020-07-03 | 上海波赛统环境科技有限公司 | Medicament and method for removing nitrogen and phosphorus from eutrophic water body by microorganisms |
| CN113186105A (en) * | 2021-03-25 | 2021-07-30 | 北京利泽恒环保科技有限公司 | Kitchen waste degradation composite microorganism and preparation method thereof |
| CN114105286A (en) * | 2020-08-31 | 2022-03-01 | 中国石油化工股份有限公司 | Device and method for high-temperature biochemical treatment of petrochemical wastewater |
| CN114105398A (en) * | 2020-08-31 | 2022-03-01 | 中国石油化工股份有限公司 | Device and method for treating petrochemical wastewater by high-temperature fluid bed biochemical system |
| WO2022142950A1 (en) * | 2020-12-29 | 2022-07-07 | 中海油天津化工研究设计院有限公司 | Active sludge regulator for use in biochemical processing of refinery wastewater |
| CN115594308A (en) * | 2021-06-28 | 2023-01-13 | 中国石油化工股份有限公司（Cn） | High-temperature bacterium domestication method and biochemical device |
-
2003
- 2003-09-15 CN CN 03133995 patent/CN1242935C/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104891648A (en) * | 2015-07-07 | 2015-09-09 | 成都营星科技有限公司 | Cultivation method of active sludge for treating three-compound combination flooding oily sewage |
| CN111362421A (en) * | 2019-12-30 | 2020-07-03 | 上海波赛统环境科技有限公司 | Medicament and method for removing nitrogen and phosphorus from eutrophic water body by microorganisms |
| CN114105286A (en) * | 2020-08-31 | 2022-03-01 | 中国石油化工股份有限公司 | Device and method for high-temperature biochemical treatment of petrochemical wastewater |
| CN114105398A (en) * | 2020-08-31 | 2022-03-01 | 中国石油化工股份有限公司 | Device and method for treating petrochemical wastewater by high-temperature fluid bed biochemical system |
| WO2022142950A1 (en) * | 2020-12-29 | 2022-07-07 | 中海油天津化工研究设计院有限公司 | Active sludge regulator for use in biochemical processing of refinery wastewater |
| CN113186105A (en) * | 2021-03-25 | 2021-07-30 | 北京利泽恒环保科技有限公司 | Kitchen waste degradation composite microorganism and preparation method thereof |
| CN115594308A (en) * | 2021-06-28 | 2023-01-13 | 中国石油化工股份有限公司（Cn） | High-temperature bacterium domestication method and biochemical device |
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|---|---|
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