CN1592608A

CN1592608A - Micropherules containing a pleuromutilin derivative

Info

Publication number: CN1592608A
Application number: CNA028233050A
Authority: CN
Inventors: S·C·威兰-贝格豪森; F·J·拉克齐; B·M·克龙－埃克哈特
Original assignee: Novartis AG
Current assignee: Elanco Tiergesundheit AG
Priority date: 2001-11-28
Filing date: 2002-11-27
Publication date: 2005-03-09
Anticipated expiration: 2022-11-27
Also published as: CN100348185C; WO2003045354A8; KR20040058334A; US8236851B2; DE60220436D1; KR100965017B1; CA2466671A1; US20050070486A1; AU2002356740B2; BR0214521A; NZ533085A; ZA200403801B; AU2002356740A1; RU2296004C2; EP1450767A1; ATE363270T1; WO2003045354A1; EP1450767B1; PT1450767E; RU2004119815A

Abstract

The provision of animal feed pellets is described, which pellets comprise, as antibiotic, a pleuromutilin derivative in stabilised form, namely in the form of microspherules. The pleuromutilin derivatives in question have the general formula (I) wherein R1 is ethyl or vinyl, there is either a double bond or a single bond between carbon atoms 1 and 2, Ra and Rb are each independently of the other hydrogen or halogen, and T is a short or long-chain organic radical.

Description

The microsphere that contains pleuromutilin derivative

The present invention relates to contain the pleuromutilin derivative of stable form as antibiotic animal feed grain (pellet).The preparation method that the present invention also relates to the preparation method of stable pleuromutilin derivative, above-mentioned animal feed grain with and purposes in control animal infectious disease method.

Hereinafter, pleuromutilin derivative can be interpreted as that to contain as shown in the formula the I macrocyclic structure be the chemical compound of feature,

R wherein ₁Be ethyl or vinyl, between carbon atom 1 and 2 or be two keys or be singly-bound that Ra and Rb are separate, are respectively hydrogen or halogen, and T is preferably the organic group of short chain as will be detailed later or long-chain.

In some documents, term pleuromutilin, ten thousand Buddhist nun's rhzomorphs, taimulin and mutilins use as synonym.All the time use term in this article.

Concerning the veterinary, pleuromutilin is to use the most general and effective antibiotic at present.The most representative known Tiamutin that hereinafter describes that comprises ^Taimulin) and up-to-date Econor (active substance: ^(active substance: ten thousand Buddhist nun's rhzomorphs).The two all can treat animal breath system and gastral all bacterial infection diseases very effectively, even is also showing its effectiveness under having to conventional antibiotic those thorny situations that medication combined heavy dose of mode is used with various active because produce drug resistance.

Effective bacterium spectrum of pleuromutilin comprises the aronson as Streptococcus, staphylococcus aureus (Staphylococcus aureus), mycoplasma arthritidis (Mycoplasma arthritidis), mycoplasma bovigenitalium (Mycoplasma bovigenitalium), Mycoplasma bovimastitidis, mycoplasma bovirhinis (Mycoplasma bovirhinis), the kind of Mycoplasma, mycoplasma canis (Mycoplasmacanis), leopard cat mycoplasma (Mycoplasma felis), mycoplasma fermentans (Mycoplasmafermentans), mycoplasma gallinarum (Mycoplasma gallinarum), Mycoplasma gallisepticum (Mycoplasma gallisepticum), A.granularum, mycoplasma hominis (Mycoplasmahominis), mycoplasma hyorhinis (Mycoplasma hyorhinis), Actinobacillus laidlawii, mycoplasma meleagridis (Mycoplasma meleagridis), mycoplasma neurolyticum (Mycoplasmaneurolyticum), the pathogen of mycoplasma pneumoniae (Mycoplasma pneumonia) and mycoplasma hyopneumoniae (Mycoplasma hyopneumoniae).

In addition, WO 98/01127 has described the disease syndrome that ten thousand Buddhist nun's rhzomorphs take place domesticated animal anywhere all remarkable activity, as in order to transport, to occupy very narrow space (having increased the density of storage) and therefore bears the animal of higher pressure.In this prevailing pathogen with decisive role is mycoplasma hyopneumoniae, Serpulina (before being Treponema) hyodysenteria, Serpulinapilosicoli, Lawsonia intracellularis, Mycoplasma gallisepticum, multocida (Pasteurella multocida), Actinobacillus (Haemophilus) pleuropneumoniae and haemophilus parasuis (Haemophilus parasuis), often also forms complicated clinical symptoms with respiratory tract and other infectious disease simultaneously.To all domestic animals such as cattle, sheep and pig, comprise that also poultry is all effective.

In current breed as the domestic animal of pig, cattle, horse, sheep and poultry on a large scale, for mentioned Animal diseases, it is impossible not giving antibiotic, unless because treat, otherwise disease is through animal rapid spread by the gross, thereby causes unnecessary loss.Therefore, people are badly in need of can effectively control the antibiotic of infectious disease between animal fast before a large amount of herds infect.

Although pleuromutilin is satisfying all expectations of people as antibiotic aspect the effect, but they have the common drawback that can not despise, be their relative instability under form of medication, and this administering mode is because easy operating is particularly important for the veterinary.As discussing in WO 01/41758, the pleuromutilin of pleuromutilin, especially free alkali form is unstable especially, and this makes that people have to adopt the acid-addition salts form in injection solution, is preferably hydrochlorate.Acid-addition salts at room temperature can be stablized preservation 5 years.Oral administration form for animals but is an exception so far, even as feed additive, also only is applied in the limited range.

And the human antibiotic can be extremely different the form of medication administration, as tablet, sugar coated tablet, Emulsion, injection solution etc., because can depend on patient's submissiveness and think the hope of rehabilitation, but for animal, this aspect but is the quite actual problem that will face.

In animal, must prepare a kind of prepared product that can take medicine.Certainly, thus it also is possible giving in the mode that animal has to swallow or inject that antibiotic treats single animal or a small amount of animal.Yet these pressure modes can not because this is high-intensity work, be needed the veterinary on the scene under individual cases by large quantities of animals received, finally cause expensive, these costs because the competition existence and can't impute to meat or milk product consumer.Therefore, in large-scale animal farm, need simple and reliable form of medication, the rancher just can independent operation or even full automation, and cost control is in reasonable range.

A kind of method that addresses all of these issues is for mixing the antibiotic of accurate dosage in the dry feed (just said feedstuff grain) of animal.

Current domestic animal and productive livestock, as pig, and cattle, sheep and poultry are often raised in the animal house that is equipped with go-go full-automatic feeding system.In this case, the amount of fully can automatization ground distributing feedstuff and every day will determine every day in the time of determining according to animal age and body weight is transported to each animal and is inserted in its trough.Said feedstuff grain often is applied in this full automation system.This grain is the concentrated dry feed of plant and/or animal base, can add as the additive of albumen, vitamin and mineral and strengthen.This feedstuff grain is simple synthetic granule length or the garden with flowability, perhaps according to different production methods, be processed into the shaft-like of the unified size specification that conforms to the age with the kind of animal, from being used for several millimeters of poultry about 1 centimetres to be used for growing up pig and cattle.Commercial feed factory is by grinding Organic Ingredients, composition is mixed into required compositions and is pressed into the granular feedstuff grain produced at last; Pack into the feedstuff grain in the bag and transport to those it is added into the rancher of distribution system.Because this grain has uniform specification, flowable and bin stability, the great advantage of this grain is an easy operating.Can be easily it be poured out in the container full automation, metering is divided into aliquot, via conveyer belt or pipe will be just accurately the feedstuff grain of amount be transported to each animal.And feedstuff grain comparatively fresh feedstuff takies less space, the most important thing is, animal can be enjoyed and of one's own accord without any problem.

Therefore, not only with protein and other as the nutrient of vitamin and mineral but also required antibiotic is added those feedstuff intragranulars all is useful.In practice, used this mode, but in the situation of the pleuromulins active substance of this discussion, this mode is faced with some concrete difficulties, these difficulties are special for this type of material, will make an explanation in more detail hereinafter.

Have been found that pleuromutilin is a bit unstable, at first, in the preparation of feedstuff grain when with feedstuff raw material (especially plant and animal fiber) when contact, quite major part has just been lost in preparation.In the preparation of feedstuff grain, animal derived or the plant-derived organic material of doing is ground, fully mix with additive, vitamin, trace element, antibiotic (being pleuromutilin derivative in the case) etc., just mix full and uniformly, then with the water or the water vapour moistening of about 5-10% weight, press down at the high pressure of the high temperature of about 60-80 ℃ (preferably 65-75 ℃), about 1-100kbar (using 25-100kbar always) and to make granule.Persistent high temperature (as 100 ℃) greatly reduces the viscosity of granule to producing unfavorable effect.On the contrary, reaching 200 ℃ the partial temperature peaks of short-term in this pressure is that said flash-point is no problem.The time that material stops under this pressure is about 5-180 second, preferred 10-90 second, can decide according to granule is big or small.

And pure pleuromutilin can tolerate this temperature well, can at room temperature deposit even some months and without any the loss of detectable active substance, but under pressure and with feedstuff in the animal or plant fiber contact closely the back and high temperature under, their decomposition very fast relatively.Seemingly with the contact catalysis of fiber catabolic process.Even in the possible scope of technology very short with the control of high temperature, high pressure phase, immediately the feedstuff grain of making is cooled to room temperature after the pressing process, still have the active component of 1/4-1/3 to lose.Although catabolite is to the animal treated and have no adverse effect, inevitably the loss of active substance must cause the increase of appreciable composite cost.

In addition, the applicant also have been found that pleuromutilin also intact in the feedstuff grain with as pure active substance to compare bin stability poorer.Make the active component in the feedstuff grain even at room temperature still continue degraded.After three months, active component content has been reduced to and has been less than 60%.Now, this relative unstability means that also the accurate dosage of the active substance in the feedstuff grain only maintains the feedstuff grain and makes the about 4-6 in back in this period of time in week.Therefore, rancher now must not be not only with the feedstuff grain of just having made.They can not a large amount of feedstuff grain of long term store, guarantees that the new effectively supply of the feedstuff of antibiotic content that contains is really arranged so the circumferential feed factory of about every 4-6 of having to sends new product order.Although this is feasible technically, in fact its needs accurate administrative plan, and makes the feed factory repeat fulfillment of having to be not suitable for the small amount order of their scheduling of production, and causes the waiting time of inconvenience, especially causes the extra increase of feedstuff grain cost.

Therefore, in view of the foregoing, aspect pleuromutilin stability, doing a lot of effort, making it can the withstand high temperatures high pressure in feedstuff grain preparation process and the loss of non-activity material, and the feedstuff grain of making has the long term storage stability that is suitable for practical application.

The example that this stability is attempted failure comprises: (1) dwindles the surface area of active component by being pressed into granule, has attempted the granule of all size; (2) seal above-mentioned active ingredient particle with various protective layers, as gelatin or various sugar and coating; (3) with various porous masses active component is wrapped up, have or do not have extra protective layer as cellulose, starch, silicic acid or zeolite; And the chemical modification of the basic macrocyclic structure of (4) active substance.Although chemical modification has improved the stability of chemical compound itself in some cases, also make loss of activity simultaneously.

Yet these are attempted no one and accomplish can reduce the loss of active component or bigger improvement is arranged on bin stability when compacting feedstuff grain.Yet the applicant has obtained wonderful success now, makes that user can be with the feedstuff grain with the administration easily of a kind of like this form, and promptly above-mentioned shortcoming no longer appears in active component wherein.Be surprisingly, may make pleuromutilin stable at present, these pleuromutilins not only can be resisted the destruction in the feedstuff grain preparation process, and preservation in can be during extremely long.

Although describe the present invention with reference to the specific embodiment of ten thousand Buddhist nun's rhzomorphs hereinafter,, equally also have the pleuromutilin derivative of the basic macrocyclic structure of formula I shown in this paper beginning applicable to Tiamutin /taimulin and other.

In the context of the present invention, be preferably as follows the pleuromutilin of formula I, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt

R wherein ₁Be ethyl or vinyl;

(A) between the carbon atom 1 and 2 be singly-bound, Ra and Rb are H, and T is in the following a-i group any one:

a)-CH ₂-OH；

C)-(CH ₂-X) _m-(CH ₂) _n-N (R ₂) (R ₃), wherein X be-O-,-S-,-NH-or M is 0 or 1; N is the integer of 2-5; R ₂And R ₃It is separate,

Be respectively C _1-6Alkyl, perhaps form with the nitrogen-atoms that they connected contain-S-,-O-or-N (R ₄)-(be R wherein ₄Be C _1-6Alkyl or C _1-6Five yuan or hexa-member heterocycle of hetero moiety hydroxyalkyl), and Y and Z are separate, are respectively-O-or-S-;

D)-CH ₂-S-(CH ₂) _k-N (R ₅) (R ₆), wherein k is the integer of 2-5; R ₅And R ₆Separate, be respectively C _1-6Alkyl;

E)-CH ₂-S-C (CH ₃) ₂-CH ₂-NH-C (O)-R ₇, R wherein ₇For-NH ₂The C that replaces _1-6Alkyl, perhaps for saturated contain one or two and be selected from-S-and-the heteroatomic five-ring heterocycles of NH-;

F)-CH ₂-S-C (CH ₂) _l-R ₈, wherein l is 0 or 1, and R ₈For

Wherein K is H, C _1-6Alkyl sulphonyl ,-NH ₂,-CHO ,-N (R ₉) (R ₁₀) ,-S-(CH ₂) _q-N (R ₉) (R ₁₀) or-C (G)-NHR ₁₁, G is oxygen or sulfur, R ₉And R ₁₀Separate, be respectively H, C _1-6Alkyl, C _1-6Alkyl sulphonyl; C _1-6Hydroxyalkyl, C _1-6Dihydroxyalkyl or unsubstituted or C _1-6The C that alkyl sulphonyl replaces _1-6Alkanoyl; Or R ₉And R ₁₀Form piperazinyl unsubstituted or that replace with the nitrogen-atoms that they connected, wherein second nitrogen-atoms can be by being selected from C _1-6Alkyl, C _1-6Hydroxyalkyl and C _1-6The substituent group of dihydroxyalkyl replaces; R ₁₁Be C _1-6Alkyl or C _1-6Alkyl-carbonyl; Q be H ,-NH ₂,-CF ₃, C _1-6Alkyl, pyridine radicals or-N (R ₉) (R ₁₀), R ₉And R ₁₀As mentioned above;

G)-CH ₃,-CH ₂Cl, CH ₂Br ,-CH ₂SCN ,-CH ₂-NH ₂,-CH ₂-N ₃,-CO-OH ,-CH ₂-OCOCH ₃Or

H)-N (R ₁₅) (R ₁₆), R wherein ₁₅And R ₁₆Identical or different, be selected from H, not saturated the or unsaturated C of straight or branched that replaces or replace _1-6The group of alkyl; Saturated or the unsaturated C that does not replace or replace _3-8Group of naphthene base; The heterocycle that does not replace or replace; And the aromatic yl group that does not replace or replace; Perhaps R ₁₅And R ₁₆With the nitrogen-atoms that they connected form one do not contain other hetero atoms or contain be selected from-N-,-O-and-the heteroatomic ternary of S-is to octatomic ring; Perhaps R ₁₅Be one of above-mentioned group, and R ₁₆For-SO ₂R ₁₇,-C (O) R ₁₈,-O-R ₁₉Or N (R ₁₉) (R ₂₀); R ₁₇Be selected from the saturated or unsaturated C of straight or branched that does not replace or replace _1-6Hydrocarbyl group, the not saturated or unsaturated C that replaces or replace _3-8Group of naphthene base, the not heterocycle that replaces or replace, the not aromatic yl group that replaces or replace, the not C that replaces or replace _1-6Alkylamino group and the arylamino group that does not replace or replace; R ₁₈Be selected from H, not saturated the or unsaturated C of straight or branched that replaces or replace _1-6Hydrocarbyl group, the not saturated or unsaturated C that replaces or replace _3-8Group of naphthene base, heterocycle that does not replace or replace and the aromatic yl group that does not replace or replace; R ₁₉And R ₂₀Identical or different, be selected from the saturated or unsaturated C of straight or branched that does not replace or replace _1-6The group of alkyl, the not saturated or unsaturated C that replaces or replace _3-8Group of naphthene base, the heterocycle that does not replace or replace and the aromatic yl group that does not replace or replace, perhaps R ₁₉And R ₂₀With the nitrogen-atoms that they connected form one optional contain be selected from-N-,-O-and-the heteroatomic ternary of S-is to octatomic ring shape group;

(B) between the carbon atom 1 and 2 be two keys, and Ra and Rb be H, T is following group i:

I)-CH ₂-CO-R ₁₂, R wherein ₁₂Be nitrogenous five yuan or the hexa-member heterocycle that do not replace or replace, replace or the substituted aryl group or-CH ₂-R ₁₃Group, R ₁₃For halogen or-SR ₁₄, and R ₁₄Be amino-C _1-6Alkyl, nitrogenous five yuan or hexa-member heterocycle that does not perhaps replace or replace or the aromatic yl group that does not replace or replace, the substituent group of above-mentioned heterocycle or aromatic yl group is to be selected from 1-3 to be selected from following group: OH, CN, NO ₂, N ₃, C _1-6Alkyl, C _1-6Alkoxyl, C _1-6Alkoxy-C _1-6Alkyl, two-N-C _1-6Alkyl amino, C _1-6Acyl amino, C _1-6Acyl group carbonylamino, C _1-6Acyloxy, C _1-6Carbamoyl, one-N-and two-N-C _1-6Alkyl-carbamoyl, C _1-6Acidic group carbonyl, C _1-6Alkyl sulphonyl, C _1-6Alkyl sulfinyl and benzyl;

(C) between the carbon atom 1 and 2 be singly-bound, Ra is H, OH or F, and Rb is H; Perhaps Ra is that H and Rb are F, and T is following group k:

K)-CH ₂-CO-R ₁₂, R wherein ₁₂I defines as group.

By known method, the free cpds of formula I can be converted into its acid-addition salts, and vice versa.In acid-addition salts, hydrochlorate most preferably.Same by known method itself, can prepare quaternary ammonium salt.

Unless otherwise indicated, substituent definition is based on the content of technical staff's common sense in general chemistry field.In the context of following formula I, according to the number of carbon atom, alkyl itself or be methyl, ethyl, n-pro-pyl, isopropyl, normal-butyl, sec-butyl, the tert-butyl group, isobutyl group etc. as other substituent parts." halogen " is fluorine, chlorine, bromine or iodine; Preferably fluorine, chlorine or iodine and more preferably chlorine.

Saturated or unsaturated five yuan or the hexa-member heterocycle that preferably contain one or more hetero atoms (preferred sulfur and nitrogen).More preferably contain 1,2 or 3 nitrogen-atoms and do not have other heteroatomic this heterocyclic subunit groups.Wherein, especially preferred those only contain a nitrogen-atoms as heteroatomic unsaturated five yuan or hexa-member heterocycle, as pyridine, pyrroles and 5, and 6-dihydro-3H-pyrroles.Suitable unsaturated five yuan or the hexa-member heterocycle that contains two nitrogen-atoms is for as imidazoles, pyridazine and pyrimidine.Also can there be one or more phenyl ring to condense on this ring with it.Benzimidazole, quinoline, isoquinolin and 2 are typical case.Suitable contain five yuan of three nitrogen-atoms or hexa-member heterocycle for as 1,2, the 4-triazole.The heterocyclic group that preferably contains a nitrogen-atoms and a sulphur atom in addition.Comprise as various thiazoles, 4 5-thiazoline and benzothiazole.1,3, the 4-thiadiazoles is the heterocyclic typical case that contains two nitrogen-atoms and a sulphur atom.Unless stated otherwise, aryl or aromatic yl group (especially phenyl or naphthyl) can be unsubstituted or can carry nearly 4 and be selected from following identical or different substituent group: OH, nitro, amino, cyano group, halogen, C _1-6Alkyl, C _1-6Alkoxyl, C _1-6Alkoxy-C _1-6Alkyl, halo C _1-6Alkyl, one-N-C _1-6Alkyl amino, two-N-C _1-6Alkyl amino, C _1-6Acidic group, C _1-6Acylamino-, C _1-6Acyl group carbonylamino, C _1-6Carbamoyl, one-N-and two-N-C _1-6Alkyl-carbamoyl, C _1-6Acyloxy carbonyl, C _1-6Alkyl sulphonyl, C _1-6Alkyl sulfinyl and benzyl.Unless stated otherwise, suitable substituent group is identical with the substituent group of bar ring, and is same, and heterocyclic radical also can replace one or many by identical or different group.Especially preferred heterocyclic radical is: 3-pyridine radicals, 4-pyridine radicals, pyrimidine-2-base, 1,3,4-thiazol-2-yl, benzothiazole-2-base, 2H-1,2,4-triazole-3-base, azabicyclo heptyl, azabicyclo octyl group and piperidyl.

The invention particularly relates to formula I chemical compound, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein R as giving a definition ₁Being vinyl, is singly-bound between the carbon atom 1 and 2, and Ra and

Rb is a hydrogen or halogen, preferred hydrogen, and T defines suc as formula I.

Be preferably as follows the pleuromutilin derivative of the formula I of definition especially, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein

R ₁Be vinyl; It between the carbon atom 1 and 2 singly-bound;

Ra and Rb are H, and

T is-CH ₂-S-(CH ₂) _k-N (R ₅) (R ₆), wherein k is the integer of 2-5; And R ₅And R ₆Separate, be respectively C _1-6Alkyl.Wherein, most preferably T is-CH ₂-S-(CH ₂) ₂-N (C ₂H ₅) (C ₂H ₅) pleuromutilin derivative.

Be preferably as follows the pleuromutilin derivative of the formula I of definition equally, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein

R ₁Be vinyl; It between the carbon atom 1 and 2 singly-bound;

Ra and Rb are H, and T is-CH ₂-S-C (CH ₃) ₂-CH ₂-NH-C (O)-R ₇, R wherein ₇For-NH ₂The C that replaces _1-6Alkyl or saturated contain one or two and be selected from-S-and-the heteroatomic five-ring heterocycles of NH-.Wherein, preferred T is-CH ₂-S-C (CH ₃) ₂-CH ₂-NH-C (O)-R ₇Pleuromutilin derivative, R wherein ₇For-NH ₂The C that replaces _1-6Alkyl, more preferably T is-CH ₂-S-C (CH ₃) ₂-CH ₂-NH-C (O)-CH (NH ₂)-CH (CH ₃) ₂Pleuromutilin derivative.

Therefore, in the context of the present invention, preferred compound taimulin and Wan Ni rhzomorph, in view of its broad spectrum of activity, ten thousand Buddhist nun's rhzomorphs most preferably.As mentioned above, two kinds of medicines for commercial applications.The chemical constitution of these two kinds of preferred agents is as follows:

Described formula I chemical compound in the document in detail, following only for reference:

R wherein ₁Define suc as formula I with A, the formula I chemical compound that defines among T such as the group a is obtained by separation such as Kavanagh and at Proc.Natl.Acad.Soc.37,570-574 (1951) describes.The basic previous generation of this chemical compound has shown this class material, the i.e. pleuromutilin in this discussion.The architectural feature of pointing out the pleuromutilin found after a while in United States Patent (USP) 4 247 542 is that the Y among the above-mentioned formula I is-CH ₂-OH.Same United States Patent (USP) has also been described R ₁Being vinyl, is singly-bound between the carbon atom 1 and 2, and Ra and Rb are H, and T is-CH ₂-β, the formula I chemical compound of D-xylopyranosyl.

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group b and describes in United States Patent (USP) 4 129 721.

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group c and describes in United States Patent (USP) 4 148 890.

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group d and describes in United States Patent (USP)-3 919 290, comprises the taimulin of mentioning especially several times, and its commodity are called Tiamutin .

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group e and describes in European patent 0 153 277, comprises ten thousand Buddhist nun's rhzomorphs of mentioning especially several times and also having understood from WO 98/01127.

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group f and describes in United States Patent (USP)-4 428 953.

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group g and describes in United States Patent (USP)-3 979 423.

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group h and describes in WO97/25309.

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group I and describes in WO01/14310.

R wherein ₁Define suc as formula I with A, T is defined as the formula I chemical compound of group k and describes in WO01/14310.

Be appreciated that each embodiment that mentions especially includes in the preferred embodiment of the invention in these lists of references.

Disclose a series of tests in the document, but these tests can not solve the technical problem that the present invention proposes with medicine (comprising taimulin) adding animal feed.Following concise and to the point some reference material of discussing:

European patent 0 165 577 relates to the feed additive that contains the zinc bacitracin, and when mixing the back with feedstuff or granulating, the zinc bacitracin has strengthened stability and may prolong the resting period.By providing polymeric coatings to make stability obtain to improve for the feed additive that contains the zinc bacitracin, be used as the polymeric coatings of zinc bacitracin as polysaccharide, polyacrylate, fat, fats compound or paraffin.

1991.04.26 Derwent Publication XP002197610[JP 03 101619 ASDS BIOTECH CORP] relate to a kind of veterinary drug of particle form of the no bitterness of forming by Zeolite support and taimulin.

1988.02.13 Derwent Publication XP002198060[JP 63 033330 ANIPPON KAYAKU KK] relate to the Perorally administrable antimicrobial medicine form of medication of taimulin, based on sodium polyacrylate, the active component absorbtivity is strengthened.Form of medication comprises powder, and it also can be pressed into granule or tablet and mix with animal feed.

European patent 0 707 798 has been described the preparation method of the feedstuff that contains active medicinal matter.The method is characterized in that, active medicinal matter is sparged with sprayable gel form on the feedstuff for preparing with physical method separately or with the mixture in the preparation of suitable lid human relations gel form.

European patent application EP-0 658 313 has been described the granule by core and coating preparation.Core is prepared from by organic substance or the inorganic substances as plant, and diameter is 100-800 μ.Coating is prepared from by the water-soluble polymer that contains active component, polymer water soluble wherein, gastric juice especially.Active component mixed in the coating or with its adhesion.In process of production, at first prepare core.The aqueous solution of active component is sprayed in the surface acid treatment of core then.Purpose is that preparation can be without difficulty and the blended fine grained of animal feed.Therefore but opposite with the present invention, the granule described in this list of references can not make active component have remarkable stability, is not suitable for containing the preparation of the animal feed grain of pleuromutilin derivative.Moreover, also different with the present invention is that active component discharges at gastric.

The applicant now is surprised to find, can pleuromutilin derivative be wrapped in the microsphere by known method; These microspheres can be mixed in the dry aminal feed and under High Temperature High Pressure, be pressed into the feedstuff grain, oven dry subsequently, and do not have the loss of inevitable active substance of the prior art.And active substance has very high stability in the new feedstuff grain that obtains, so the bin stability of final feed is high.At room temperature, the sustainable some months of depositing of this feedstuff grain that is to say that the content of active component is constant basically.

The active substance that is comprised in the microsphere not only makes stability that the raising of never expecting has been arranged; and with the comparing of pure active substance; more help microsphere and do not form dust, do not form agglomerate, have significant flowability and protect active substance to avoid unnecessary ectocine.Therefore, for example can avoid sucking unintentionally or contacting in operation skin and eyes.Because active substance is contained in the microsphere, so, just in case wrongly taking, the people used, can handle simple and safely and do not need to adopt special safeguard procedures.Because microsphere does not have cohesive to the surface of equipment basically, and neither form also bonding never in any form of agglomerate or scleroma, so in Feed Manufacturing factory, can have no the used equipment of technical difficulty ground cleaning; Simple vacuum cleaned is enough usually.

And, use these microspheres also to have additional advantage.When giving antibiotic, in responsive patient, find that therapeutic process has increased the weight of the appetite decline when oral.The order of severity that the doctor can be according to circumstances so changes other the not form of medication by stomach into, as injection or suppository.In animal, under individual cases, found same phenomenon.The appetite decline shows as the feedstuff that refusal is eaten q.s.And the metabolism of feedstuff is relatively poor, desirable weight increase also do not occur.Because the feedstuff of these animals eat still less, so oral medication is also invalid.For animal, can not select to be transformed into suppository, and injection has above-mentioned shortcoming, purpose of the present invention will address these problems exactly.According to the present invention, when oral application microsphere, therefore the refusal feed of not finding said allergy and occurring together is inferred relevant with the acid-resisting of the substrate of microsphere.

Studies show that of bioavailability, microsphere can be intact by stomach, and active component only discharges in the enteric cavity of alkaline environment.In piglets, carry out following contrast feeding experiment: use the feedstuff grain or the commercial ECONOR  that a) contain free hydrochloric acid ten thousand Buddhist nun's rhzomorphs to carry out; and use b) carry out according to embodiment 2 prepared feedstuff grains; this feedstuff grain is enclosed with hydrochloric acid ten thousand Buddhist nun's rhzomorphs in microsphere; per hour extract the blood sample of experimental animal and measure the concentration of blood plasma Nei Wanni rhzomorph; find: under situation a); after administration 2-3 hour, the concentration of active component raises rapidly and reaches peak.After 8-10 hour, curve begins to fall after rise and is approaching zero.The b that is handling with microsphere) under the situation, the concentration that postpones active component after about 1-2 hour begins to increase, and reaches maximum after about 3-4 hour, begins after about 10-12 hour to fall after rise to zero.Therefore, although at b) situation time of being issued to effective plasma level concentration postpones a little,, this is to treatment adverse effect not.This new method has produced valuable administering mode, and this administering mode is non-stimulated and make oral medication more effective to stomach.If desired, by adding additive, microsphere of the present invention is dissolved and release of active agent in the sour environment of stomach as sodium bicarbonate.Yet, be unwanted as a rule.

In the context of the present invention, microsphere can be understood as the spherical polymeric matrices granule that small, the most microgranule mean size of diameter is about 1-5000 μ m, is generally 50-3000 μ m.Pleuromutilin derivative is contained in wherein.Therefore, they are minimum spheroids, comprising polymeric matrices closely, and described active component with solid or liquid form high degree of dispersion in this binding matrix, and be not only by coating.Also may be described as a kind of special encapsulation form.

The method itself for preparing microsphere among the present invention is known; Equally, be used to prepare capsular raw material and used pleuromutilin derivative also is known.Yet, with this kind mode prepare microsphere be the initiative, therefore, contain the feedstuff grain of these microspheres and in animal the oral usage of anti-infectious disease all be new.

The preparation of microsphere can be carried out with the method that is similar in the following document of mentioning:

Shigeru Goto etc., Journal of Microencapsulation, 1986, the 3 volumes, the 4th phase, 293-305 page or leaf;

Shigeru Goto etc., Journal of Microencapsulation, 1986, the 3 volumes, the 4th phase, 305-316 page or leaf or United States Patent (USP) 3 714 065 (corresponding to DE-2 105 039).

The present invention relate to aspect the oral application of the preparation of microsphere or pleuromutilin less, and emphasis of the present invention is at new feedstuff grain, this feedstuff grain contains the pleuromutilin derivative of stable existence in microsphere, the therefore forfeiture of no tangible active component in preparation or storage.It is stable to the invention reside in the active component that makes in the feedstuff grain.The present invention attempts to help the those skilled in the art to solve existing technical problem, can in considerable time, store the feedstuff grain that contains pleuromutilin and give the method for family-raise and productive livestock for it provides, not need a large amount of personnel, time and logistics simultaneously these feedstuff grains.At last, not only save time in practice and money but also fairly obvious safety and the reliability of having increased.

In the two-phase system that forms by organic facies or organic-water (first phase) and oil phase (second mutually), can prepare microsphere easily.Organic or organic-water is made up of solution or dispersion liquid, solvent and the pleuromutilin derivative to be wrapped of the polymerization composition that is suitable for forming microsphere.Oil phase is the dispersion liquid of dispersive aluminum monostearate, aluminium distearate, Aluminium Tristearate Micronized sterile, sodium stearate, calcium stearate or magnesium stearate in suitable oil, and described suitable oil is preferably liquid paraffin or silicone oil.In addition, also available nonionic is as the dispersant or the emulsifying agent of dehydration minashi sugar alcohol list olein (Span-80 ).The oil phase volume is more preferably greater than several times of organic facies volumes.Make biphase abundant mixing by powerful stirring, even can be issued to homogenize in the help of high pressure or static mixer.In this process, form small polymer particle.The active component that comprises the high dispersive form in the microsphere, and microsphere do not dissolve in reactant mixture, so can pass through decantation or isolated by filtration, washing and drying.

Biphase stirring is also very important for forming microsphere.Usually, can use the agitating device of the stirring rod with propeller shape, rotating speed is at least about 100-1500rpm, to guarantee two alternate sufficient mixing and to form microsphere rapidly.Certainly, also can use static mixer.

The detailed step of preparation microsphere is as follows:

(a) preparation is suitable for forming the polymer solution of microsphere, polymer is selected from lac and is polymer based with cellulose, acrylic or methacrylic acid, maleic anhydride, polyvinylpyrrolidone or polyvinyl alcohol, this can be by having low-affinity and dielectric constant to be about in the organic solvent of 10-40 the dissolving lac or polymer carries out to paraffin oil or silicone oil, in the time of suitably, Ke Jiashui;

(b) under agitation pleuromutilin derivative is incorporated in lac or the polymer solution, forms not the first mutually organic facies miscible with paraffin oil or silicone oil;

(c) under powerful stirring (as using static mixer or high-pressure homogenizer), mix second phase (oil phase of forming by paraffin oil or silicone oil) mutually with first, and continue to stir the gained mixture up to solvent evaporation or remove the back and form the microsphere that contains pleuromutilin derivative;

(d) separate, and if be fit to, can wash and dry microspheres.

In pharmaceuticals industry, lac is known, and can be used for preparing the sugar-coat of the sugar coated tablet of natural flavor.

Concerning the polymer of cellulose base, suitable raw material is Cellacefate or N, N-di-n-butyl hydroxypropyl ether acetic acid cellulose.

The used raw material of the polymer of acrylic or methacrylic acidic group is as methacrylate/methacrylic acid copolymer, 2-methyl-5-vinyl-pyridine/methacrylate/methacrylic acid copolymer, methyl methacrylate/methacrylic acid copolymer, methyl methacrylate/maleic anhydride copolymers or methyl methacrylate/maleic anhydride copolymers.

Concerning the polymer of maleic anhydride base, suitable raw material is ethylene methyl ether/maleic anhydride copolymers or styrene/maleic anhydride copolymers.In the context of the present invention, the polymer of preferred especially acrylic or methacrylic acidic group is as the shell of microsphere.Preferably use commercial product to be prepared.These products are the product of acrylic acid and acrylic ester polymerization, wherein contain a small amount of quaternary ammonium group.Commercial product from Eudragit  E, the L of the R hm of German Darmstadt or S is very suitable.Eudragit  E is the cation type polymer of dimethylaminoethyl methacrylate and neutral methacrylate.Eudragit  L and S are the anionic polymers of methacrylic acid and methyl methacrylate.

Concerning polyvinylpyrrolidone--based polymer, suitable raw material is a polyvinylpyrrolidone.

Concerning PVA-based polymer, suitable raw material is a polyvinyl alcohol itself.

Use quite a large amount of oil phases, make the volume ratio of organic facies/oil phase be about 1/20-5/10.

Usually, operating under promptly about 20 ℃ of-45 ℃ of temperature under room temperature or the slightly high temperature.Yet room temperature is fully enough.

Concerning the first phase organic facies, suitable organic solvent is not miscible as far as possible and volatile solvent with oil phase.The dielectric constant of these solvents at 10-40 for the most suitable.In following table example many this solvents.

Solvent dielectric constant solvent dielectric constant

Methanol 32.6 phenol 9.8

Ethanol 24.3 acetone 20.7

Isopropyl alcohol 18.7 acetic acid 9.7

Butanols 17.1 anhydride 20.7

Benzyl alcohol 13.1 Nitrocarbol .s 35.9

Ethylene glycol 37.7 ethylenediamines 14.2

Propylene glycol 35.0 sour cellosolves 16

The mixture of available single solvent or these solvents is as acetone-alcohol mixture (1: 1).Add a spot of water and can reach preferable effect, the water of promptly about 1-5 part volume is joined the organic solvent of 10-50 part volume.Preferred acetone-water mixture (about 30: 1).

Verified, it is favourable adding aluminum monostearate, aluminium distearate or preferred Aluminium Tristearate Micronized sterile, sodium stearate, calcium stearate, magnesium stearate or other emulsifying agent or dispersants as dehydration minashi sugar alcohol list olein (Span-80 ) nonionic before use in second phase (oil phase), and powerful stirring makes dispersion liquid even.This addition manner has especially promoted the quick formation of microsphere of uniform size.The formed microsphere of result has prevented in preparation process each other coalescent.When first of the pleuromutilin derivative that contains polymer is prepared from by acetone-water mixture and during with biphase mixing, can obtains 50-1000 μ m microsphere of uniform size so under the rotating speed of 800-1000rpm stirs.The weight ratio of pleuromutilin and stearate is preferably 0.5/1-10/1, more preferably from about 1: 1.

Available ether, petroleum ether or normal hexane, methyl cyclopentane or preferred cyclohexane extraction washing microsphere.Under vacuum, room temperature, extremely leniently remove solvent.Self-evident, residue should be low as much as possible behind the removal of solvents.

By the microsphere that said method obtained firm relatively polymeric shell is arranged.In order to obtain bigger flexibility, can in organic facies, add the plasticizer of about 3-10%, based on the polymer weight meter.Suitable plasticizer is a glyceryl triacetate; Acetylizad monoglyceride; Glycerol; Polyethylene Glycol as PEG400 or PEG600; Phthalic acid ester as diethyl phthalate or dibutyl phthalate; Citrate as triethyl citrate, citric acid acetyl triethyl, tributyl citrate or citric acid acetyl tributyl; And as the vegetable oil of Oleum Ricini, Oleum Brassicae campestris or Oleum Helianthi.Can use the triethyl citrate of about 4-10%.Yet, needn't add plasticizer usually, because found that the ratio of plasticizer is high more, the bin stability of final feed grain is poor more.Therefore, have the opposite effect in the improvement that is added on stability of plasticizer, but also do not mean that, can not add plasticizer, especially the plasticizer of relatively small amount.Plasticizer has reduced glass transition temperature.So according to the inventor's experience, when glass transition temperature was lower than about 100-150 ℃, active component no longer was protected in the preparation of feedstuff grain.

Often produce the feedstuff grain by feed factory.Usually be substrate with powdery corn.In this substrate, add extra composition as oil and plant and animal proteinum.All the components grind or mixing apparatus in fully mix, water sprinkling or steam treatment and at high temperature by the round nozzle extruding of the about 2-15mm of diameter are promptly suppressed.In pressing process, wet raw material is compacted, and leaves nozzle with hard relatively bar-shaped form, is cut into the piece of required length, 5-25mm length according to appointment at nozzle exit with cutter sweep.The still warm feedstuff grain that obtains at air drying, perhaps is put on the conveyer belt dry down at about 80-120 ℃ by heating clamber in the process that betransported away.The feedstuff grain of making is bar-shaped or cylindric; They have slick relatively surface and are easy to topple over and do not have fragmentation or dust and form.Their common density is about 1.2g/cm ³

Usually, according to the present invention, the method for the feedstuff grain of preparation stabilisation is not identical with the preparation method of the common feedstuffs grain that does not add medicine.But, no matter be which kind of method, before pressing process, all need the organic feed composition of microsphere and Powdered homogenize is fully mixed, with the water of about 5-10% weight or steam is moistening and be pressed into the feedstuff grain under the high temperature of about 60-80 ℃ (preferably 65-75 ℃).When the feedstuff grain at first with after all the other feed ingredients of relative fraction are fully mixed, when obtaining the premix of a so-called microsphere that contains sizable ratio thus, then help most the homogenization that reaches good.Then, a part of premix is mixed the other mixture of formation with remaining feedstuff, in the end a step, this part mixture is diluted to ultimate density with other feedstuff.This dilution makes the active component of feedstuff intragranular parcel distribute even especially.

To store or be transported to final consumer in feedstuff grain cool to room temperature and pack into paper bag or other proper container.Need not special preventive measure because the feedstuff grain when storing the highly stable and active component that contained in one deck coating, thereby prevented the influence of outer bound pair active component.The non-activity composition can successfully overflow the outside of the feedstuff grain of these storages.

Mensuration in the front and back that are pressed into the feedstuff grain to the amount of active component shows that the microsphere that uses pelletize to prepare does not cause the loss of any detectable active component astoundingly.

The enforcement of embodiment

Embodiment 1: the preparation of hydrochloric acid ten thousand Buddhist nun's rhzomorphs of methacrylic resin parcel

Composition weight

Hydrochloric acid ten thousand Buddhist nun's rhzomorph (active substance) 12.5g

Excipient

EudragitL?100*?????????????????????????????????37.5g

Aluminum monostearate 11.25g

Water 9.4g

Acetone 303.1ml

Liquid paraffin,light 1250ml

Gross weight 1351.94g

* Eudragit  is the commercial product of R hm.It is prepared from by butyl methacrylate, methacrylic acid (2-dimethyl aminoethyl) ester and methylmethacrylate copolymer.

Step 1:Under room temperature, the stirring (800rpm/5 minute/magnetic stirring apparatus), in glass beaker, Eudragit is scattered in the 100ml acetone.Continue dispersed with stirring liquid under the same conditions and add entry.After 10 minutes, polymer dissolves fully.Continue simultaneously to stir by part this active substance of adding ten thousand Buddhist nun's rhzomorphs.After 10 minutes, obtain limpid solution.

Step 2:In 2 liters of reactors that have 3 sword spiral splash bars (1000rpm), at room temperature aluminum monostearate is scattered in the liquid paraffin,light.Dispersion liquid is even after 10 minutes.

Step 3:At room temperature, the solution that will be obtained in step 1 adds in the dispersion liquid that step 2 obtained, and continues simultaneously to stir (1000rpm).At room temperature formed emulsion was further stirred (800rpm) 24 hours.(perhaps, also can at first emulsion be heated to 40 ℃ in 1 hour, under the pressure of 200mbar, keep then 2 hours).In both cases, all form the microsphere that has wrapped up active substance, i.e. the microsphere that is prepared from by methacrylic resin.

Step 4:After stopping to stir, microsphere sinks to the bottom of reactor, as far as possible fully pours out the paraffin and the aluminum monostearate on upper strata.With cyclohexane extraction washing microsphere (3 times/buchner funnel/the fabric filtration film) and remove excessive cyclohexane extraction in a vacuum several times.

Embodiment 2: be used for the preparation of the feedstuff grain of swine rearing: (little pig feed)

Fully mix in the piglets dry feed of the Powdered homogenizing that active substance (ten thousand Buddhist nun's rhzomorphs) the adding 3920g of 80g is commonly used and with crutcher.Obtain the premix of 4000g by this method.Premix with 4000g in 100 liters of ribbon mixers joins in the piglets dry feed of other 36kg Powdered homogenizing commonly used, fully mixes with the same manner.Then, the partially mixed thing of resulting 40kg is mixed in the piglets dry feed of other 360kg Powdered homogenizing commonly used, changes in the extruder and under 68-72 ℃, 10-100kbar pressure, be pressed into the bar-shaped feedstuff grain that about 10mm is long, 6mm is wide.In pressing process, use steam (2bar, 136 ℃).Retention time at the extruder heating part is about 75 seconds.The grain of making is packed in each bag with every bag of 25kg.

Embodiment 3: by the active component or the microsphere coating active of free active component, commercial coating The stability test of the forage mixture that becomes to be grouped into

According to preparation embodiment 2, with different pretreatment but the active component of same amount preparation three kinds of piglets feedstuff grain A, B and C.A type feedstuff grain comprises commercial ECONOR  50% (active component is ten thousand Buddhist nun's rhzomorphs), and its active component wraps up with hydroxypropyl emthylcellulose (HPMC).Type B feedstuff grain comprises single-activity composition hydrochloric acid ten thousand Buddhist nun's rhzomorphs, and C type feedstuff grain comprises the prepared microsphere that contains hydrochloric acid ten thousand Buddhist nun's rhzomorphs according to preparation embodiment 1.

For the mensuration of stability,, and after finishing, preparation takes out first sample immediately, every kind of each 50g according to three kinds of feedstuff grains of preparation embodiment 2 preparations.Get each 9 in the sample of A type sample, Type B, need prepare the feedstuff grain of three kinds of different batches for the C type, slightly different on the composition of feedstuff each other.Equally, from each batch, get 9 samples.Check all samples immediately and pass through to analyze the content of determining undegradable ten thousand Buddhist nun's rhzomorphs.The residual feed grain is divided into two parts, changes in the case of two climate changes and be used to study for a long period of time.Case (I) is 25 ℃, and relative humidity is 60%, the normal storage under the analog room temperature.Case (II) is 40 ℃ of high temperature, 75% high relative humidity, the situation of simulation long term store.

With one month interval, each model from each case chamber and batch the feedstuff grain in take out the sample of 3 each 50g, and measure the content of undegradable ten thousand Buddhist nun's rhzomorphs.

List meansigma methods and the standard deviation of being measured under the different weather conditions thereof as following table 1 and 2.

Table 1:25 ℃/relative humidity 60%

Data [ten thousand Buddhist nun's rhzomorph %/(standard deviations)]

	After the pelletize at that time	After one month	After two months	After six months
	After the pelletize at that time	After one month	After two months	After six months	The A type	98.36％	76.68％	70.54％	37.31％

ECONOR 50％，HPMC	/(9.28)	/(2.56)	/(1.38)	/(1.39)
ECONOR 50％，HPMC	/(9.28)	/(2.56)	/(1.38)	/(1.39)	Type B hydrochloric acid ten thousand Buddhist nun's rhzomorphs	78.38％ /(8.66)	43.55％ /(15.37)	47.30％ /(1.00)	26.62％ /(0.87)
The C type is contained in ten thousand Buddhist nun's rhzomorphs in the microsphere	102.93％ /(6.49)	99.69％ /(3.18)	99.20 /(2.11)	96.22％ /(3.91)		78.38％ /(8.66)	43.55％ /(15.37)	47.30％ /(1.00)	26.62％ /(0.87)

Table 2:40 ℃/relative humidity 75%

Data [ten thousand Buddhist nun's rhzomorph %/(standard deviations)]

	After the pelletize at that time	After one month		After two months	After six months
	After the pelletize at that time	After one month		After two months	After six months	A type ECONOR  50%, HPMC	98.36％ /(9.28)	38.72％ /(2.28)	25.35％ /(1.13)	6.83％ /(0.96)
Type B hydrochloric acid ten thousand Buddhist nun's rhzomorphs	76.38％ /(8.66)		34.65％ /(15.98)	15.33％ (0.24)	9.14％ /(0.90)	A type ECONOR  50%, HPMC	98.36％ /(9.28)	38.72％ /(2.28)	25.35％ /(1.13)	6.83％ /(0.96)
	76.38％ /(8.66)		34.65％ /(15.98)	15.33％ (0.24)	9.14％ /(0.90)	The C type is contained in ten thousand Buddhist nun's rhzomorphs in the microsphere	102.93％ (6.49)	96.42％ /(1.74)	89.12％ /(3.19)	79.70％ /(6.62)

Last table shows fully aware ofly, and the stability that is present in ten thousand Buddhist nun's rhzomorphs in A, B and the C type feedstuff grain is different.Pure ten thousand Buddhist nun's rhzomorphs (Type B) are degraded the fastest significantly, and have lost about 21% in granulation process.After two months, ten thousand Buddhist nun's rhzomorph content in the Type B are reduced to and are less than 50% under common room temperature, and under 40 ℃ of high temperature even reduce to and be less than 20%.The A type carries out coating with HPMC to ten thousand Buddhist nun's rhzomorphs, and the degraded of ten thousand Buddhist nun's rhzomorphs is lacked really, but still quite big.The loss of 1% active component can be ignored in granulation process, still, stores after two months down at 25 ℃, then causes about 30% remarkable loss, at 40 ℃ down even reach about 76%.On the contrary, the active component active ingredient loss that is contained in the C type feedstuff grain in the microsphere obviously reduces.Under 25 ℃, after two months, only lose approximately 1%, under 40 ℃ of high temperature, only lose about 11%.Under the situation of C type even after 6 months, almost 80% active component still exists, and the content of active component is reduced to below 10% under other two kinds of situations.

People can't expect that the stability of the active component in the feedstuff grain significantly increases, and especially can not produce any Stabilization because mix microsphere in the feedstuff of not compacting.In the feedstuff of not compacting, the unprotected ten thousand Buddhist nun's rhzomorphs and the microsphere Nei Wanni rhzomorph form of expression are identical and lose identical.

Remarks: when the application submitted to, above-mentioned test was still underway; Other data will obtain in coming few months.

Claims

1. the pleuromutilin derivative of stable following formula I,

R wherein ₁Be ethyl or vinyl, between carbon atom 1 and 2 or be two keys or be singly-bound that Ra and Rb are separate, are respectively hydrogen or halogen, and T is the organic group of short chain or long-chain; Its Chinese style I chemical compound is wrapped in the microsphere.

2. according to the stable pleuromutilin derivative in the microsphere of being wrapped in of claim 1, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein in formula I

R ₁Be ethyl or vinyl;

a)-CH ₂-OH；

C)-(CH ₂-X) _m-(CH ₂) _n-N (R ₂) (R ₃), wherein X be-O-,-S-,-NH-or

M is 0 or 1; N is the integer of 2-5; R ₂And R ₃Separate, be respectively C _1-6Alkyl, perhaps form with the nitrogen-atoms that they connected contain-S-,-O-or-N (R ₄)-five yuan or hexa-member heterocycle of hetero moiety, at-N (R ₄) in, R ₄Be C _1-6Alkyl or C _1-6Hydroxyalkyl, and Y and Z are separate, are respectively-O-or-S-;

E)-CH ₂-S-C (CH ₃) ₂-CH ₂-NH-C (O)-R ₇, R wherein ₇For-NH ₂The C that replaces _1-6Alkyl or for saturated contain one or two and be selected from-S-and-the heteroatomic five-ring heterocycles of NH-;

F)-CH ₂-S-C (CH ₂) _l-R ₈, wherein l is 0 or 1, and R ₈For

Wherein K is H, C _1-6Alkyl sulphonyl ,-NH ₂,-CHO ,-N (R ₉) (R ₁₀) ,-S-(CH ₂) _q-N (R ₉) (R ₁₀) or-C (G)-NHR ₁₁, G is oxygen or sulfur, R ₉And R ₁₀Separate, be respectively H, C _1-6Alkyl, C _1-6Alkyl sulphonyl; C _1-6Hydroxyalkyl, C _1-6Dihydroxyalkyl, unsubstituted or C _1-6The C that alkyl sulphonyl replaces _1-6Alkanoyl; Or R ₉And R ₁₀Form piperazinyl unsubstituted or that replace with the nitrogen-atoms that they connected, wherein second nitrogen-atoms can be by being selected from C _1-6Alkyl, C _1-6Hydroxyalkyl and C _1-6The substituent group of dihydroxyalkyl replaces; R ₁₁Be C _1-6Alkyl or C _1-6Alkyl-carbonyl; Q be H ,-NH ₂,-CF ₃, C _1-6Alkyl, pyridine radicals or-N (R ₉) (R ₁₀), R ₉And R ₁₀As mentioned above;

H)-N (R ₁₅) (R ₁₆), R wherein ₁₅And R ₁₆Identical or different, be selected from H, not saturated the or unsaturated C of straight or branched that replaces or replace _1-6Alkyl; Saturated or the unsaturated C that does not replace or replace _3-8Group of naphthene base; The heterocycle that does not replace or replace; And the aromatic yl group that does not replace or replace; Perhaps R ₁₅And R ₁₆With the nitrogen-atoms that they connected form one do not contain other hetero atoms or contain be selected from-N-,-O-and-the heteroatomic ternary of S-is to octatomic ring; Perhaps R ₁₅Be one of above-mentioned group, and R ₁₆For-SO ₂R ₁₇,-C (O) R ₁₈,-O-R ₁₉Or N (R ₁₉) (R ₂₀); R ₁₇Be selected from the saturated or unsaturated C of straight or branched that does not replace or replace _1-6Hydrocarbyl group, the not saturated or unsaturated C that replaces or replace _3-8Group of naphthene base, the not heterocycle that replaces or replace, the not aromatic yl group that replaces or replace, the not C that replaces or replace _1-6Alkylamino group and the arylamino group that does not replace or replace; R ₁₈Be selected from H, not saturated the or unsaturated C of straight or branched that replaces or replace _1-6Hydrocarbyl group, the not saturated or unsaturated C that replaces or replace _3-8Group of naphthene base, heterocycle that does not replace or replace and the aromatic yl group that does not replace or replace; R ₁₉And R ₂₀Identical or different, be selected from the saturated or unsaturated C of straight or branched that does not replace or replace _1-6Hydrocarbyl group, the not saturated or unsaturated C that replaces or replace _3-8Group of naphthene base, the heterocycle that does not replace or replace and the aromatic yl group that does not replace or replace, perhaps R ₁₉And R ₂₀With the nitrogen-atoms that they connected form one optional also contain be selected from-N-,-O-and-the heteroatomic ternary of S-is to octatomic ring shape group;

I)-CH ₂-CO-R ₁₂, R wherein ₁₂Be nitrogenous five yuan or the hexa-member heterocycle that do not replace or replace, the aromatic yl group that replaces or replace or-CH ₂-R ₁₃Group, wherein R ₁₃For halogen or-SR ₁₄, and R ₁₄Be amino-C _1-6Alkyl perhaps is nitrogenous five yuan or hexa-member heterocycle that does not replace or replace or the aromatic yl group that does not replace or replace, and the substituent group of above-mentioned heterocycle or aromatic yl group is 1-3 and is selected from following group: OH, CN, NO ₂, N ₃, C _1-6Alkyl, C _1-6Alkoxyl, C _1-6Alkoxy-C _1-6Alkyl, two-N-C _1-6Alkyl amino, C _1-6Acyl amino, C _1-6Acyl group carbonylamino, C _1-6Acyloxy, C _1-6Carbamoyl, one-N-and two-N-C _1-6Alkyl-carbamoyl, C _1-6Acidic group carbonyl, C _1-6Alkyl sulphonyl, C _1-6Alkyl sulfinyl and benzyl;

K)-CH ₂-CO-R ₁₂, R wherein ₁₂Such as among the group i definition.

3. according to the stable pleuromutilin derivative in the microsphere of being wrapped in of claim 2, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein R ₁Being vinyl, is singly-bound between the carbon atom 1 and 2, and Ra and Rb be H or halogen, and T is suc as formula defining among the I.

4. according to the stable pleuromutilin derivative in the microsphere of being wrapped in of claim 3, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein

R ₁Be vinyl; It between the carbon atom 1 and 2 singly-bound;

Ra and Rb are H, and

T is-CH ₂-S-(CH ₂) _k-N (R ₅) (R ₆), wherein k is the integer of 2-5; And R ₅And R ₆Separate, be respectively C _1-6Alkyl.

5. according to the stable pleuromutilin derivative in the microsphere of being wrapped in of claim 4, wherein T is-CH ₂-S-(CH ₂) ₂-N (C ₂H ₅) (C ₂H ₅).

6. according to the stable pleuromutilin derivative in the microsphere of being wrapped in of claim 3, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein

R ₁Be vinyl; It between the carbon atom 1 and 2 singly-bound;

Ra and Rb are H, and

T is-CH ₂-S-C (CH ₃) ₂-CH ₂-NH-C (O)-R ₇, R wherein ₇For-NH ₂The C that replaces _1-6Alkyl or saturated containing be selected from-S-and-one or two heteroatomic five-ring heterocycles of NH-; Comprise that the physiology goes up acceptable acid-addition salts and quaternary ammonium salt thereof.

7. according to the stable pleuromutilin derivative in the microsphere of being wrapped in of claim 6, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein

R ₁Be vinyl; It between the carbon atom 1 and 2 singly-bound;

Ra and Rb are H, and

T is-CH ₂-S-C (CH ₃) ₂-CH ₂-NH-C (O)-R ₇, R wherein ₇For-NH ₂The C that replaces _1-6Alkyl.

8. according to the stable pleuromutilin derivative in the microsphere of being wrapped in of claim 7, comprise that its physiology goes up acceptable acid-addition salts and quaternary ammonium salt, wherein

R ₁Be vinyl; It between the carbon atom 1 and 2 singly-bound;

Ra and Rb are H, and

T is-CH ₂-S-C (CH ₃) ₂-CH ₂-NH-C (O)-CH (NH ₂)-CH (CH ₃) ₂

9. according to any one stable pleuromutilin derivative that is wrapped in the microsphere among the claim 1-8, wherein microsphere is made up of the polymer that is suitable for forming microsphere, and described polymer is selected from lac and cellulose, acrylic or methacrylic acid, maleic anhydride, polyvinylpyrrolidone or PVA-based polymer.

10. prepare the method for the microsphere that contains pleuromutilin derivative any among the claim 1-9, this method comprises:

(a) preparation is suitable for forming the polymer solution of the substrate of microsphere, described polymer is selected from lac and is polymer based with cellulose, acrylic or methacrylic acid, maleic anhydride, polyvinylpyrrolidone or polyvinyl alcohol, this is by in that paraffin oil or silicone oil are had low-affinity and dielectric constant for dissolving lac or polymer in the organic solvent of about 10-about 40 carry out, and adds water in the time of suitably;

(b) under agitation pleuromutilin derivative is incorporated in lac or the polymer solution, formation and paraffin oil or silicone oil immiscible first is organic facies mutually;

(c) under powerful stirring, mix mutually in the second phase oil phase of forming by paraffin oil or silicone oil, and continue to stir the gained mixture contains pleuromutilin derivative up to solvent evaporation or the formation of removal back microsphere first;

(d) separate, and if be fit to washing and dry microspheres.

11. animal feed grain, this feedstuff grain is except that the feedstuff that comprises pulverous dried plant and/or animal base, also comprise stable pleuromutilin derivative, and add or do not add additive, as albumen, vitamin and mineral according to effective dose any among the claim 1-9.

12. be used for the treatment of the animal feed grain in the method for infectious disease of domestic animal and productive livestock according to claim 11.

13. be used for the treatment of purposes in the animal feed grain of infectious disease of domestic animal and productive livestock in preparation according to any one stable pleuromutilin derivative that is wrapped in the microsphere among the claim 1-9.

14. preparation contains the preparation method of the animal feed grain of stable pleuromutilin derivative any among the with good grounds claim 1-9, this method comprises fully mixes the stable pleuromutilin derivative according to microspheres form any among the claim 1-9 with the organic feed composition of Powdered homogenize, with the water of about 5%-10% weight or steam is moistening and it is bar-shaped to be pressed into, and these rods are divided into the feedstuff grain under about 60 ℃-80 ℃ high temperature.