CN1583070A - Medicine for improving sleep and nourishing bowel and relieving constipation and its preparation - Google Patents
Medicine for improving sleep and nourishing bowel and relieving constipation and its preparation Download PDFInfo
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Abstract
A Chinese medicine in the form of solution for improving sleep and treating constipation is prepared from 5 Chinese-medicinal materials including citron with tip endings like finger, astragalus root, white atractylodes rhizome, wild jujube kernel, etc. through pulverizing, extracting and proportionally preparing solution.
Description
Technical field
The present invention relates to a kind of medicine, particularly a kind of energy improves medicine of sleep, loosening bowel to relieve constipation and preparation method thereof.
Background technology
Sleep is a kind of function of human brain, and " sleeping apparatus " arranged in the human brain.The excitement of brain cell and suppress to be is coordinated mutually, and brain needs excitement also to need to suppress, and destroys nervous system in order to avoid lose balance, causes the neurasthenia, causes " sleeping apparatus " malfunctioning and bring insomnia.Modern urbanite's operating pressure is big, and long-term brain overwork makes nerve in tension for a long time, the excitatory substance that discharges in the brain is too much, cause the nervous system overwork, make the excitatory state of brain be difficult to be repaired normally and suppress, thereby cause insomnia.People such as long-term insomnia will cause that each system is badly damaged in the body; the significant opportunity that insomnia can make us lose and repair body injury night, recover immunity, energy reserve on the physiology; and the destruction immune function of human body, influence the normal function of brain and people's attention, contemplative faculty.Severe patient can cause hypomnesis, inappetence etc., and with symptom such as irritated, forgetful, weak, absent minded, dejected.Studies show that according to the relevant person, the people who did not have enough sleep every day 4 hours, its mortality rate is higher by 180% than the people who slept every day 7～8 hours, and the people who did not often have enough sleep 4 hours falls ill easily, and not sleeping as continuous 5 days to cause death.The general employing of the medicine of Cure for insomnia taken sleeping pill at present.Use low dose of sleeping pill, treatment in a short time may play a role, and the memory function but the life-time service sleeping pill not only can cause damage is brought out senile dementia, and can cause drug dependence type, addiction, increase the weight of insomnia and psychotic symptom on the contrary.Take in large quantities for a long time, headache can also occur, emotionally be reluctant communication, symptoms such as strange taste, gait difficulty, fatiguability and liver function damage in mouthful.
In addition, along with growth in the living standard, outeat, eat the assorted modern's of becoming diet custom, but have absorption that discharge is just arranged, this just meets balance principle.Defecation is smooth, the embarrassment of constipation and painful but in fact a lot of people are standing.Secular constipation can not be discharged timely because of the harmful substance that produces in the body, thereby causes self poisoning symptoms such as abdominal distention, loss of appetite, mouthful interior abnormal flavour, has a strong impact on healthy.Some constipation patients often use charthartic also to claim cathartic.Charthartic mainly is by stimulating or promote enterokinesia, causing defecation reflex or the medicine by softening feces, lubricating intestinal, make intestinal contents be easy to excrete.By its model of action, be divided three classes substantially: a class is an osmotic laxative, as magnesium sulfate, sodium sulfate; Another kind of is contact cathartics, as phenolphthalein; Also having a class is emollient laxative, as liquid paraffin, glycerin etc.But the side effect that these cathartics produce is bigger.Take also the shortage that causes calcium and vitamin in the body because of losing of calcium and vitamin for a long time.
Summary of the invention
The objective of the invention is to the problem that heavily exists at background technology, a kind of medicine that can improve sleep, loosening bowel to relieve constipation is provided.
Another object of the present invention is to provide a kind of preparation method that can improve the medicine of sleep, loosening bowel to relieve constipation.
Fructus Citri Sarcodactylis acrid in the mouth hardship warm in nature is returned liver, spleen, stomach, lung meridian, have soothing liver-QI, regulate the flow of vital energy and in, clears phlegm.Modern pharmacy studies show that Fructus Citri Sarcodactylis contains chemical constituents such as bergapten, Fructus Citri Limoniae lactone, flavonoid, cupreol, daucosterol, succinic acid, pharmaceutical research shows to have the gastrointestinal movement of the impelling rhythm and pace of moving things, promote effect such as intestinal contents propelling, and clinical research shows that diseases such as constipation are had better curative effect.Radix Astragali nature and flavor temperature.Function invigorating QI to consolidate the body surface resistance, diuretic detumescent, toxin expelling granulation promoting.Be mainly used in the disease of deficiency of both the splenic and pulmonary QI or sinking of QI of middle-JIAO, be applicable to symptoms such as lack of appetite, loose stool, shortness of breath and fatigue, chronic diarrhea proctoptosis.Modern pharmacy studies show that the Radix Astragali contains active strong triterpene modern pharmacology and studies show that the Radix Astragali has pharmacodynamics effect very widely; to immune system, cardiovascular system, blood and hemopoietic system; antagonist metabolism, anti-ageing waiting for a long time all have good effect; aspect the digestion systemic effect, mainly showing as the dilatability that strengthens small intestinal (mainly being jejunum) motion and smooth muscle; experiment type hepatitis there is protective effect, also has antibiotic, antivirus action in addition.Rhizoma Atractylodis Macrocephalae nature and flavor sweetness and bitterness is returned spleen, kidney channel, has air making-up and spleen enlivening, dampness diuretic, the antiabortive merit of hidroschesis.Be applicable to deficiency-weakness of spleen-QI, dysfunction of the spleen in transportation and transformation institute is to symptoms such as lack of appetite, loose stool, distension and fullness in the abdomen, lassitudes.Modern pharmacology studies show that the Rhizoma Atractylodis Macrocephalae is rich in volatile oil, acetylene compound, also contains several amino acids such as fructose, inulin, Rhizoma Atractylodis polysaccharide, serine, glutamic acid and vitamin A etc.Pharmaceutical research shows.The Rhizoma Atractylodis Macrocephalae has inhibitory action to experimental gastric ulcer, and can reduce gastric acidity and pepsin output, can protect gastric mucosa, and the small intestinal conveying function is had facilitation, also has effects such as function of gallbladder promoting, hepatoprotective, raising immunity, antitumor in addition.Clinical research shows that the Rhizoma Atractylodis Macrocephalae has good efficacy to the treatment constipation, and is particularly better to the weakness constipation.The Caulis Polygoni Multiflori sweet in the mouth, property is flat, GUIXIN, Liver Channel, the function tranquilizing by nourishing the heart, collateral dredging dispels the wind, and can be used for diseases such as insomnia, dreaminess, hyperhidrosis, blood deficiency limbs pain.Modern pharmacological research shows that it has sedative-hypnotic effect, and total sleep time is prolonged, and prolongs mutually when mainly being chronic sleep, and the paradoxical sleep phase shortens, and clinical research report Caulis Polygoni Multiflori has better curative effect to insomnia and dreamful sleep due to the deficiency of YIN insufficiency of blood.The flat sweet in the mouth of Semen Ziziphi Spinosae, the GUIXIN lung meridian has the tranquilizing by nourishing the heart effect, is applicable to diseases such as insomnia, palpitation with fear, forgetful, dryness of the mouth and throat, cold sweating, night sweat, the few tongue of red tongue, is Chinese traditional treatment insomnia medicine commonly used, and clinical report shows that Semen Ziziphi Spinosae has good soporific function.
A kind of medicine that can improve sleep, loosening bowel to relieve constipation of the present invention adopts following materials of weight proportions:
Fructus Citri Sarcodactylis 5～40 Radixs Astragali 5～40 Rhizoma Atractylodis Macrocephalaes 5～40
Caulis Polygoni Multiflori 5～40 Semen Ziziphi Spinosaes 5～40
The optimization formula of medicine of the present invention:
Fructus Citri Sarcodactylis 10～30 Radixs Astragali 10～30 Rhizoma Atractylodis Macrocephalaes 10～30
Caulis Polygoni Multiflori 10～30 Semen Ziziphi Spinosaes 10～30
The optimum formula of medicine of the present invention:
Fructus Citri Sarcodactylis 20 Radixs Astragali 15 Rhizoma Atractylodis Macrocephalaes 14
Caulis Polygoni Multiflori 12 Semen Ziziphi Spinosaes 12
Described medicine is made said dosage form on any pharmaceutics; Preferred for preparation becomes solution.
A kind of preparation method that can improve the medicine of sleep, loosening bowel to relieve constipation comprises the steps:
(1), Fructus Citri Sarcodactylis, the Radix Astragali are pulverized, add ethanol again and carry out alcohol reflux, filtering extracting liquid leaves standstill medicinal liquid cold preservation, draws supernatant then, reclaims ethanol, and medicinal liquid is standby;
(2), the medicinal residues that obtain after filtering in the Rhizoma Atractylodis Macrocephalae, Semen Ziziphi Spinosae, Caulis Polygoni Multiflori and the step (1) are put in the extraction pot, add water and be heated to and boil, filter, filtered solution is concentrated, add ethanol precipitation then, cold preservation is left standstill again, draw supernatant, reclaim ethanol to there not being the alcohol flavor, medicinal liquid is standby;
(3), obtain medicinal liquid in combining step (1) and the step (2), add water in right amount again, cold preservation is left standstill, and draws supernatant again, joins full dose with pure water, packing, and sterilization is promptly.
The medicinal liquid that obtains in an amount of Mel and oligofructose and step (1), the step (2) can also be merged in the step (2), add water in right amount, cold preservation is left standstill, and draws supernatant again, joins full dose with pure water, packing, and sterilization is promptly.
Medicine of the present invention is made up of Fructus Citri Sarcodactylis, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Caulis Polygoni Multiflori, Semen Ziziphi Spinosae, adopts modern science and technology to make, and has the effect of improving gastrointestinal tract, loosening bowel to relieve constipation and improvement sleep; The function supplementing QI and nourishing YIN is calmed the nerves, and loosening bowel to relieve constipation is applicable to poor sleep, the intestines and stomach discord, constipation etc., and suitable patient takes for a long time.
The specific embodiment
Embodiment 1: take by weighing raw material (kilogram) by following proportioning
Fructus Citri Sarcodactylis 20 Radixs Astragali 15 Rhizoma Atractylodis Macrocephalaes 14
Caulis Polygoni Multiflori 12 Semen Ziziphi Spinosaes 12
According to the above-mentioned raw materials proportioning oral liquid that to be prepared into 100,000 content be 10ml, its preparation method is as follows:
(1), Fructus Citri Sarcodactylis, the Radix Astragali are pulverized, add 80% ethanol of 5 times of amounts again, reduced-pressure backflow extracts 2 times (temperature is controlled at 60 ℃～70 ℃), each 2 hours, filtering extracting liquid then, cold preservation was left standstill more than 24 hours, draw supernatant then, reclaim ethanol, medicinal liquid is standby;
(2), the medicinal residues that obtain after filtering in the Rhizoma Atractylodis Macrocephalae, Semen Ziziphi Spinosae, Caulis Polygoni Multiflori and the step (1) are put in the extraction pot, add the water of 8 times of amounts, be heated to and boil, extract 2 times, each 1 hour, filter, filtered solution merged and be concentrated into an amount of (heat is surveyed relative density and is about about 1.10), put to room temperature, add ethanol precipitation again, making the alcohol amount of containing of solution is 60%, alcohol deposit fluid cold preservation was left standstill more than 24 hours, draw supernatant, reclaim ethanol to there not being the alcohol flavor, medicinal liquid is standby;
(3), obtain medicinal liquid in combining step (1) and the step (2), adding water in right amount, cold preservation was left standstill more than 24 hours, drew supernatant again, joined full dose with pure water, packing, and 100 ℃ of flowing steam sterilizations 30 minutes are promptly.
Embodiment 2: take by weighing raw material (kilogram) by following proportioning
Fructus Citri Sarcodactylis 30 Radixs Astragali 10 Rhizoma Atractylodis Macrocephalaes 10
Caulis Polygoni Multiflori 30 Semen Ziziphi Spinosaes 10
According to the above-mentioned raw materials proportioning oral liquid that to be prepared into 100,000 content be 10ml, its preparation method is with embodiment 1.
Embodiment 3: take by weighing raw material (kilogram) by following proportioning
Fructus Citri Sarcodactylis 10 Radixs Astragali 30 Rhizoma Atractylodis Macrocephalaes 10
Caulis Polygoni Multiflori 20 Semen Ziziphi Spinosaes 15
According to the above-mentioned raw materials proportioning oral liquid that to be prepared into 100,000 content be 10ml, step (1), step (2) among the step of its preparation method (1), step (2) and the embodiment 1 are basic identical, in step (3), add and obtain the medicinal liquid merging in an amount of Mel and oligofructose and step (1) and the step (2), adding water in right amount, cold preservation was left standstill more than 24 hours, drew supernatant again, joined full dose with pure water, packing, 100 ℃ of flowing steam sterilizations 30 minutes are promptly.
Test example 1: functions of loosening bowel relieving constipation experiment
1, materials and methods
1.1 the oral liquid of the medicine of the present invention of sample embodiment 1 gained
1.2 laboratory animal
The Kunming kind secondary female mice that this experiment selects for use Chinese Academy of Medical Sciences animal center breeding farm to provide, body weight 24～26g is divided into 5 groups at random by body weight.
1.3 dosage is selected
Being tried thing is that the human body recommended dose is the approved product of 20ml/60kg.bw every day.The dose,equivalent of mice is equivalent to 10 times of human body recommended amounts.Respectively with 5 times, 10 times of the human body recommended amounts and 30 times as low (1.7ml/kg.bw), in (3.3ml/kg.bw), high (10.0ml/kg.bw) dosage.Establish blank group and model control group (compound diphenoxylate group) simultaneously.Take administration by gavage, irritate stomach every day once, 0.1ml/10g.bw was tried thing 14 days continuously, and blank group and model control group are irritated stomach equivalent distilled water every day.
1.4 instrument and reagent
Dissecting instrument, ruler, filling stomach pin, AE100 electronic balance
Carbon black ink (india ink, chemical plant in the west, Beijing, lot number 000803)
R-1132 (state-run Wujin, Jiangsu pharmaceutical factory, lot number 990225)
1.5 detection method
1.5.1 intestinal propulsion test
Tried thing continuously after 14 days, each organize the mice fasting 24 hours, except that the blank group, all the other each group is irritated stomach compound diphenoxylate 50ml/kg.bw.After 20 minutes, only irritate stomach 15%ml/ for each treated animal.Tried the thing that tried that thing group carbon black ink contains corresponding dosage.Put to death animal with the cervical vertebra dislocation method after 20 minutes.Take out whole section small intestinal to caecum portion immediately, do not add the traction tiling linearly from pylorus.Measure small intestinal total length and pylorus to the displacement of ink motion forward position, calculate the intestinal propulsion rate.
Intestinal propulsion rate=ink displacement (cm)/small intestinal total length (cm) * 100%
1.5.2 mice defecation test
Tried thing continuously after 14 days, each organized the mice fasting 24 hours.Except that the blank group, all the other each groups are irritated stomach compound diphenoxylate 10ml/kg.bw.Mice is put into mouse cage raise, normal drinking-water feed.0.5 after hour, irritate stomach 15% carbon black ink for each treated animal, 0.5ml/ only.Tried the thing that tried that thing group carbon black ink contains corresponding dosage.Every mice of observed and recorded is from irritating the stomach compound diphenoxylate, to discharging the required time of melena first, and mice defecation granule and defecation weight in 8 hours.
2. result
2.1 tried the influence of thing to the mice body weight
Table 1 is tried thing to the influence of mice body weight (X ± SD)
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | Tried the preceding body weight of thing | The P value | Tried thing jejunitas 24 hours body weight (g) after 14 days | The P value |
| Model control group | ??0 | ??12 | ??21.6±1.3 | ??—— | ??25.4±1.5 | ??—— |
| Low dose group | ??1.7 | ??12 | ??21.3±1.2 | ??0.6562 | ??25.4±2.1 | ??0.9819 |
| Middle dosage group | ??3.3 | ??12 | ??21.4±1.1 | ??0.7339 | ??25.6±2.2 | ??0.8220 |
| High dose group | ??10.0 | ??12 | ??21.5±1.3 | ??0.9755 | ??25.1±1.9 | ??0.7158 |
| The blank group | ??0 | ??12 | ??21.9±1.3 | ??0.5613 | ??25.6±1.8 | ??0.7787 |
By table 1 as seen, give being tried before and after the thing 14 days of mice various dose at per os, with the model group matched group relatively, blank group, each dosage group body weight all do not have significant difference (P＞0.05)
2.2 tried the influence of thing to the mouse small intestine ahead running
Table 2 is tried the influence of thing to mouse small intestine propelling rate
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | The intestinal propulsion rate | The P value |
| Model control group | 0 | ?12 | ?40.0±14.2 | ?—— |
| Low dose group | 1.7 | ?12 | ?48.2±15.4 | ?0.1901 |
| Middle dosage group | 3.3 | ?12 | ?51.4±11.9* | ?0.0439 |
| High dose group | 10.0 | ?12 | ?46.6±9.7 | ?0.1970 |
| The blank group | 0 | ?12 | ?77.8±10.2*** | ?1.752×10 -7 |
*: compare with model control group that there were significant differences (P＜0.05)
*: compared utmost point significant difference (P＜0.001) with model control group
By table 2 as seen, model control group is compared the intestinal propulsion rate and is reduced by 49% (P＜0.001) with the blank group, shows the modelling success.Compare with model control group, middle dosage group intestinal propulsion rate improves 29% (P＜0.05).
2.3 tried the influence of thing to the mice defecation time
Table 3 is tried the influence of thing to the mice defecation time
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | Arrange the melena time (branch) first | The P value |
| Model control group | 0 | ?12 | ?423±45 | —— |
| Low dose group | 1.7 | ?12 | ?346±70** | 0.0039 |
| Middle dosage group | 3.3 | ?12 | ?345±64** | 0.0024 |
| High dose group | 10.0 | ?12 | ?297±73*** | 4.288×10 -5 |
| The blank group | 0 | ?12 | ?108±25*** | 4.310×10 -16 |
*: compared highly significant difference (P＜0.01) with model control group
* *: compared utmost point significant difference (P＜0.001) with model control group
By table 3 as seen, model control group is compared with the blank group and is arranged 2.9 times of melena time lengthening (P＜0.001) first, shows the modelling success.Compare with model control group, basic, normal, high dosage group is arranged the melena time first and is shortened 18% (P＜0.01), 18% (P＜0.01), 30% (P＜0.001) respectively.
2.4 tried the influence of thing to mice defecation grain number
Table 4 is tried the influence of thing to mice defecation grain number
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | 8 hours defecation grain numbers (grain) | The P value |
| Model control group | 0 | ?12 | ?9±5 | —— |
| Low dose group | 1.7 | ?12 | ?16±11* | 0.0396 |
| Middle dosage group | 3.3 | ?12 | ?15±8* | 0.0024 |
| High dose group | 10.0 | ?12 | ?17±8* | 4.288×10 -5 |
| The blank group | 0 | ?12 | ?53±13*** | 4.310×10 -16 |
*: compare with model control group that there were significant differences (P＜0.05)
* *: compared utmost point significant difference (P＜0.001) with model control group
By table 4 as seen, model control group is compared 8 hours defecation grain numbers and is reduced by 83% (P＜0.001) with the blank group, show the modelling success.Compare with model control group, 8 hours defecation grains of basic, normal, high dosage group number increases by 78% (P＜0.05), 67% (P＜0.05), 89% (P＜0.05) respectively.
2.5 tried the influence of thing to mice defecation weight
Table 5 is tried the influence of thing to mice defecation weight
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | 8 hours defecation weight (g) | The P value |
| Model control group | 0 | ?12 | ?0.1225±0.0684 | —— |
| Low dose group | 1.7 | ?12 | ?0.2619±0.2014* | 0.0396 |
| Middle dosage group | 3.3 | ?12 | ?0.2268±0.1354* | 0.0024 |
| High dose group | 10.0 | ?12 | ?0.2143±0.1347* | 4.288×10 -5 |
| The blank group | 0 | ?12 | ?0.6974±0.1581*** | 4.310×10 -16 |
*: compare with model control group that there were significant differences (P＜0.05)
* *: compared utmost point significant difference (P＜0.001) with model control group
By table 5 as seen, model control group is compared 8 hours defecation weight and is reduced by 82% (P＜0.001) with the blank group, show the modelling success.Compare with model control group, 8 hours defecation weight of basic, normal, high dosage group increases by 1.1 times (P＜0.05), 85% (P＜0.05), 75% (P＜0.05) respectively.
Test example 2: the present invention improves the experiment of sleep effect
1. material and method
1.1 given the test agent: the oral liquid of the embodiment of the invention 1 gained
1.2 laboratory animal: the BALB/CI monthly age male Mus secondary mice that this experiment selects for use Chinese Academy of Medical Sciences's animal center to provide, body weight 18～22g is divided into 4 groups at random by body weight, and body weight is through T check no significant difference (P＞0.05) between group.
1.3 dosage is selected
Being tried thing is that the human body recommended dose is the approved product of 20ml/60kg.bw every day.The dose,equivalent of mice is equivalent to 10 times of human body recommended amounts.Respectively with 5 times, 10 times of the human body recommended amounts and 30 times as low (1.7ml/kg.bw), in (3.3ml/kg.bw), high (10.0ml/kg.bw) dosage.Establish blank group and model control group (compound diphenoxylate group) simultaneously.Take administration by gavage, matched group is irritated distilled water, and each dosage group gives various dose respectively and tried thing.Each is organized mice and is tried thing after 24 days, measures every index.
1.4 instrument and reagent
The AE100 electronic balance, DT500 electronic balance, stopwatch, pentobarbital sodium, barbital sodium, normal saline.
1.5 method of testing
1.5.1 direct sleep experiments
The observation animal gives various dose and is tried thing, and matched group gives with behind the volume solvent, and the phenomenon that whether occurs sleeping, sleep are index with the righting reflex loss.When mice places supine position, can right the body position immediately.Can not the person of righting as surpassing 30～60 seconds, promptly think righting reflex loss to enter sleep.Righting reflex recovers to be the animal awakening.Righting reflex loss is the animal sleep time to recovering during this period of time, writes down matched group and is tried the sleeping number of animals of thing group and the length of one's sleep.
1.5.2 prolong the inductive mouse sleep time test of pentobarbital sodium
After the animal last is tried thing, the peak effect occurred preceding 15 minutes, give each treated animal lumbar injection 32mg/kg.bw pentobarbital sodium, injection volume is 0.1ml/10g.bw.Can with the mice righting reflex loss be the sleep index, observe to be tried thing and prolong pentobarbital sodium inductive length of one's sleep.Test was carried out at night.
1.5.3 sub-threshold dose pentobarbital sodium sleep incidence rate test
After the animal last is tried thing, the peak effect occurred preceding 15 minutes, give each treated animal lumbar injection 26mg/kg.bw pentobarbital sodium, injection volume is 0.1ml/10g.bw.Reaching more than 1 minute the person with the mice righting reflex loss is sleeping standard, write down the number of animals of falling asleep in 30 minutes.Can observation is tried thing improve the inductive sleep incidence rate of pentobarbital sodium.
1.5.4 barbital sodium sleep latency test
After the animal last is tried thing, the peak effect occurred preceding 15 minutes, give each treated animal lumbar injection 240mg/kg.bw pentobarbital sodium, injection volume is 0.1ml/10g.bw.With the mice righting reflex loss is the sleep index, observes and to be tried thing to the barbital sodium preclinical shadow of sleeping.
2 results
2.1 tried the influence of thing to the mice body weight
Table 6 is tried thing to the influence of mice body weight (X ± SD)
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | Body weight (g) before the experiment | The P value | Experiment back body weight (g) | The P value |
| Matched group | 0 | ?15 | ?22.9±1.3 | ?—— | ?26.1±1.8 | ?—— |
| Low dose group | 1.7 | ?15 | ?23.4±1.3 | ?0.2904 | ?26.8±1.7 | ?0.2732 |
| Middle dosage group | 3.3 | ?15 | ?23.0±1.0 | ?0.8461 | ?27.1±1.5 | ?0.1062 |
| High dose group | 10.0 | ?15 | ?23.4±1.0 | ?0.2699 | ?26.6±1.3 | ?0.3714 |
By table 6 as seen, per os gives being tried before and after the thing 24 days of mice various dose, with matched group relatively, each dosage group body weight does not all have significant difference (P＞0.05).
2.2 tried thing to the influence of the length of one's sleep of dosage pentobarbital sodium inducing mouse above threshold
Table 7 is tried thing to the influence of the length of one's sleep of dosage pentobarbital sodium inducing mouse above threshold (X ± SD)
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | The length of one's sleep (minute) | The P value |
| Matched group | 0 | ?15 | ?27.5±11.0 | —— |
| Low dose group | 1.7 | ?15 | ?44.6±13.8*** | 0.0008 |
| Middle dosage group | 3.3 | ?15 | ?45.7±13.7*** | 0.0004 |
| High dose group | 10.0 | ?15 | ?46.4±10.0 | 3.662×10 -5 |
* *: utmost point significant difference (P＜0.001) is relatively arranged with matched group
By table 7 as seen, what per os gave the mice various dose was tried thing after 24 days, with matched group relatively, the basic, normal, high dosage group pentobarbital sodium induced hypnotic time prolongs 62% (P＜0.001), 66% (P＜0.001) and 69% (P＜0.001) respectively.
2.3 tried the influence of thing to sub-threshold dose pentobarbital sodium inducing mouse sleep incidence rate
Table 8 is tried thing to the influence of sub-threshold dose pentobarbital sodium inducing mouse sleep incidence rate (X ± SD)
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | The sleep incidence rate | The P value |
| Matched group | 0 | ?15 | ?20％ | —— |
| Low dose group | 1.7 | ?15 | ?40％ | 0.1138 |
| Middle dosage group | 3.3 | ?15 | ?47％* | 0.0384 |
| High dose group | 10.0 | ?15 | ?47％* | 0.0384 |
*: relatively there were significant differences (P＜0.05) with matched group
By table 8 as seen, what per os gave the mice various dose was tried thing after 24 days, with matched group relatively, middle and high dosage group sub-threshold dose pentobarbital sodium inducing mouse sleep incidence rate improves 1.4 times (P＜0.05) and 1.4 times (P＜0.05) respectively.
2.4 tried thing to preclinical influence of barbital sodium inducing mouse sleep
Table 9 is tried thing to preclinical influence of barbital sodium inducing mouse sleep (X ± SD)
| The animal grouping | Tried agent amount ml/kg.bw | Number of animals (only) | Sleep incubation period (minute) | The P value |
| Matched group | 0 | ?15 | ?23.6±2.6 | —— |
| Low dose group | 1.7 | ?15 | ?21.3±2.0* | 0.1138 |
| Middle dosage group | 3.3 | ?15 | ?20.1±2.0*** | 0.0384 |
| High dose group | 10.0 | ?15 | ?19.0±2.8*** | 0.0384 |
*: relatively there were significant differences (P＜0.05) with matched group
* *: utmost point significant difference (P＜0.001) is relatively arranged with matched group
By table 9 as seen, what per os gave the mice various dose was tried thing after 24 days, with matched group relatively, 10% (P＜0.05), 15% (P＜0.001) and 19% (P＜0.001) are shortened in basic, normal, high dosage group barbital sodium inducing mouse sleep incubation period respectively.
Claims (7)
1, a kind of medicine that can improve sleep, loosening bowel to relieve constipation is characterized in that comprising that following materials of weight proportions makes:
Fructus Citri Sarcodactylis 5～40 Radixs Astragali 5～40 Rhizoma Atractylodis Macrocephalaes 5～40
Caulis Polygoni Multiflori 5～40 Semen Ziziphi Spinosaes 5～40
2, a kind of medicine that can improve sleep, loosening bowel to relieve constipation according to claim 1 is characterized in that comprising that following materials of weight proportions makes:
Fructus Citri Sarcodactylis 10～30 Radixs Astragali 10～30 Rhizoma Atractylodis Macrocephalaes 10～30
Caulis Polygoni Multiflori 10～30 Semen Ziziphi Spinosaes 10～30
3, a kind of medicine that can improve sleep, loosening bowel to relieve constipation according to claim 1 is characterized in that comprising following materials of weight proportions:
Fructus Citri Sarcodactylis 20 Radixs Astragali 15 Rhizoma Atractylodis Macrocephalaes 14
Caulis Polygoni Multiflori 12 Semen Ziziphi Spinosaes 12
4,, it is characterized in that described medicine makes said dosage form on any pharmaceutics according to claim 1 or 2 or 3 described a kind of medicines that can improve sleep, loosening bowel to relieve constipation.
5, a kind of medicine that can improve sleep, loosening bowel to relieve constipation according to claim 4 is characterized in that described pharmaceutical dosage form is a solution.
6, a kind of preparation method that can improve the medicine of sleep, loosening bowel to relieve constipation according to claim 5 is characterized in that comprising the steps:
(1), Fructus Citri Sarcodactylis, the Radix Astragali are pulverized, add ethanol again and carry out alcohol reflux, filtering extracting liquid leaves standstill medicinal liquid cold preservation, draws supernatant then, reclaims ethanol, and medicinal liquid is standby;
(2), the medicinal residues that obtain after filtering in the Rhizoma Atractylodis Macrocephalae, Semen Ziziphi Spinosae, Caulis Polygoni Multiflori and the step (1) are put in the extraction pot, add water and be heated to and boil, filter, filtered solution is concentrated, add ethanol precipitation then, cold preservation is left standstill again, draw supernatant, reclaim ethanol to there not being the alcohol flavor, medicinal liquid is standby;
(3), obtain medicinal liquid in combining step (1) and the step (2), add water in right amount again, cold preservation is left standstill, and draws supernatant again, joins full dose with pure water, packing, and sterilization is promptly.
7, a kind of preparation method that can improve the medicine of sleep, loosening bowel to relieve constipation according to claim 6, it is characterized in that step (3) merges the medicinal liquid that obtains in an amount of Mel and oligofructose and step (1), the step (2), add water to an amount of, cold preservation is left standstill, draw supernatant again, join full dose with pure water, packing, sterilization is promptly.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100251434A CN100490846C (en) | 2004-06-14 | 2004-06-14 | Medicine for improving sleep and nourishing bowel and relieving constipation and its preparation |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100251434A CN100490846C (en) | 2004-06-14 | 2004-06-14 | Medicine for improving sleep and nourishing bowel and relieving constipation and its preparation |
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| Publication Number | Publication Date |
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| CN1583070A true CN1583070A (en) | 2005-02-23 |
| CN100490846C CN100490846C (en) | 2009-05-27 |
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| Application Number | Title | Priority Date | Filing Date |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512527A (en) * | 2011-12-26 | 2012-06-27 | 吕怀霞 | Traditional Chinese medicine composite for treating post-stroke depression |
| CN105412271A (en) * | 2015-11-19 | 2016-03-23 | 天津天狮生物发展有限公司 | Composition for improving sleep and preparation method thereof |
| CN106177389A (en) * | 2016-08-22 | 2016-12-07 | 宁波御坊堂生物科技有限公司 | There is loosening bowel to relieve constipation, the Chinese medicine preparation improving sleep, enhancing immunity function and preparation method thereof |
| CN106418151A (en) * | 2016-09-29 | 2017-02-22 | 广西南宁乐蕊生物科技有限责任公司 | Nutritional power capable of relaxing bowels |
-
2004
- 2004-06-14 CN CNB2004100251434A patent/CN100490846C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512527A (en) * | 2011-12-26 | 2012-06-27 | 吕怀霞 | Traditional Chinese medicine composite for treating post-stroke depression |
| CN105412271A (en) * | 2015-11-19 | 2016-03-23 | 天津天狮生物发展有限公司 | Composition for improving sleep and preparation method thereof |
| CN106177389A (en) * | 2016-08-22 | 2016-12-07 | 宁波御坊堂生物科技有限公司 | There is loosening bowel to relieve constipation, the Chinese medicine preparation improving sleep, enhancing immunity function and preparation method thereof |
| CN106177389B (en) * | 2016-08-22 | 2020-10-30 | 宁波御坊堂生物科技有限公司 | Traditional Chinese medicine preparation with functions of relaxing bowel, improving sleep and enhancing immunity and preparation method thereof |
| CN106418151A (en) * | 2016-09-29 | 2017-02-22 | 广西南宁乐蕊生物科技有限责任公司 | Nutritional power capable of relaxing bowels |
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| Publication number | Publication date |
|---|---|
| CN100490846C (en) | 2009-05-27 |
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