CN1569044A - Healthy product raw liquor of armilariella tabescens for strengthening the body and protecting the liver and method for improving the formulation of armilariella tabescens - Google Patents
Healthy product raw liquor of armilariella tabescens for strengthening the body and protecting the liver and method for improving the formulation of armilariella tabescens Download PDFInfo
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Abstract
The invention provides a health product raw liquor of armilariella tabescens for strengthening the body and protecting the liver and method for improving the formulation of armilariella tabescens, which comprises bacterial inclined-plane cultivating, liquid seed cultivating and expanded fermentation.
Description
Technical field
The present invention relates to a kind of preparation method that improves Armillaria mella tabescens (Scop.ex Fr.) Sing. output and improve the strong body hepatoprotective health product of Armillaria mella tabescens (Scop.ex Fr.) Sing. dosage form, belonged to biotechnology and pharmaceutical technology field.
Background technology
Along with industrial expansion, the human survival environment that relies on is also suffered serious pollution, many organic substances all have serious harm to each organ of human body, for example multiple artificial board, adhesive, wallpaper etc. all contain formaldehyde, formaldehyde is the potential carcinogen of generally acknowledging in the world, it stimulates eyes and respiratory mucosa etc., finally causes immunologic dysfunction, hepatic injury, injury of lung and nerve centre system to be affected; In addition, alcoholic liver disease has also reached the degree that can not be ignored in China in recent years.Ethanol is for organ injury, and is the most serious with liver, wine people people more than 300,000,000 of China, wherein the sickness rate of alcoholic liver disease, if sustain damage because of insobriety makes liver and is not is not prevented and treated about 20 percent, wherein very most of meeting changes alcoholic liver into, and the possibility of canceration is arranged.And the Armillaria mella tabescens (Scop.ex Fr.) Sing. health product can be by its unique liver protection effect to chemical hepatic injury patient, and particularly alcoholic liver disease plays the effect of protection treatment.The exploitation of strong body hepatoprotective health product can reduce hepatopath's quantity, strengthens patient's body constitution, has bigger social benefit.
After deliberation, Armillaria mella tabescens (Scop.ex Fr.) Sing. has multiple medical health care function, can not only improve the human body immunity, and chemical hepatic injury is also had curative effect preferably.Armillaria mella tabescens (Scop.ex Fr.) Sing. (armillariella tabescens [Armillariella tabescens (Scop.ex Fr.) Sing]) is a kind of fungus of finding and have independent intellectual property right for China first." Chinese medicine dictionary " record: Armillaria mella tabescens (Scop.ex Fr.) Sing. is the mycelium of white mushroom section fungus armillariella tabescens, has treatment hepatitis, cholecystitis, appendicitis, the attending effectiveness of otitis media.China's folklore is as the Chinese herbal medicine of treatment acute, chronic hepatitis and biliary tract.Main cause is to contain multiple biology effectively to reach active ingredient in its fermented product extract, as Armillaria mella tabescens (Scop.ex Fr.) Sing. first, second, third element, and Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide, polypeptide and aminoacid, trace element, natural mannitol.The polysaccharide that contains multiple heterogeneity in the Armillaria mella tabescens (Scop.ex Fr.) Sing., the immunization that can improve body also has radiation protection and leukogenic effect, and this is because Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide can quicken the synthetic of hemopoietic tissue cell DNA; Armillarisin A is a kind of coumarin derivative, has antiinflammatory, spasmolytic, analgesic function, is used for acute cholecystitis, acute, chronic hepatitis, cholelithiasis and chronic superficial, atrophic gastritis; Polypeptide and aminoacid provide rational energy substrate to keep total protein, albuminous content in the nitrogen balance raising blood plasma, make the hepatic tissue of damage be able to reparative regeneration, though aminoacid does not have immunogenicity in addition, but can play haptenic effect and macromolecular carrier and be combined into complex, can obtain immunogenicity, stimulate body to produce antibody or lymphocyte, adjust the immunity of body.Natural mannitol can improve plasma osmotic pressure, and interstitial fluid moisture is shifted and the effect of generation tissue dewatering to blood plasma, can make the hepatic ascites patient eliminate ascites rapidly.Since it was found in nineteen seventies, just be used for the treatment of hepatitis always, gastropathy and cholecystitis, its curative effect is quite sure.
Market has had armillarisin oral liquid to sell now, its product mainly adopts inexpensive and mixed preparing solid medium by a certain percentage such as Semen Maydis powder of being easy to get, sweet potato powder, disinfection inoculation, cultivated about two months in indoor lucifuge then, the taking-up back is boiled with hot water and is carried, the centrifugal removal of impurity is made finished product behind the adding adjuvant.
Its technological process of production is as follows: medium preparation---Armillaria mella tabescens (Scop.ex Fr.) Sing. inoculation fermentation---boiling water boils to be carried---the decolouring remove impurity finished product that---adds the adjuvant packing---.
But obviously the problem that exists is: can't realize industrial and large-scale production by solid fermentation 1..Generally can only carry out in 750 milliliters seed bottle with the solid medium cultivating microorganism at present, incubation time is longer, is difficult for forming the production of industrially scalable.2. also do not have the appearance of other relevant dosage form product on product forms except that armillarisin oral liquid, big but oral liquid has a taking dose, preservation term is short, shortcomings such as transportation inconvenience.
Summary of the invention
The objective of the invention is to overcome above-mentioned shortcoming, a kind of preparation method that improves the output of Armillaria mella tabescens (Scop.ex Fr.) Sing. and improve the strong body hepatoprotective health product of Armillaria mella tabescens (Scop.ex Fr.) Sing. dosage form is provided.
The present invention is achieved in that
The technical problem that invention will solve
(1) adopts liquid submerged fermentation, optimize condition of culture, shorten fermentation time greatly, mycelia is grown fully, improved production efficiency.
(2) filter out unique culture medium prescription, make Armillaria mella tabescens (Scop.ex Fr.) Sing. more effective to improving immunity and preventing and treating chemical hepatic injury through the effective ingredient of metabolic conversion.
(3) by organic solvent deposit, concentrated preparation Armillaria mella tabescens (Scop.ex Fr.) Sing. powder, make it be transformed into the health product of different dosage form easily.
(4) improve the Armillaria mella tabescens (Scop.ex Fr.) Sing. dosage form, all drawbacks of armillarisin oral liquid before overcoming, for example: active constituent content is few, and onset is slow, is difficult for transportation, difficult storage etc.
Related technological process comprises:
---------fermentation culture---is collected (fungus ball also refines and fermentation liquid)---merge extractive liquid, and fermentation liquid---decolouring---finished product (electuary, tablet, capsule) to slant culture to Armillaria mella tabescens (Scop.ex Fr.) Sing. in the liquid seeds cultivation.Manufacture method in the described technological process is:
1: the preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing. strains at different levels
A, strain inclined plane are cultivated: with the aseptic PDA inclined-plane that is connected to of strain, put into 28 ℃ of incubators and cultivated 10-15 days.
B, liquid seed culture medium are: murphy juice 20%, glucose 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, V
B1On a small quantity, PH 6.0.Configuration according to a conventional method, packing changes in the triangular flask, the sealing of jumping a queue, steam pressurized to 121 ℃ sterilization, pressurize 30min postcooling inserts the inclined-plane seed and carries out the liquid seeds cultivation to 25-28 ℃.
C, fermentation medium: glucose 3%, murphy juice 14%, Semen Maydis powder 5%, bean sprout juice 3%, ammonium chloride 0.5%, potassium dihydrogen phosphate 0.05%, calcium chloride 0.3%, PH 6.0.Configuration according to a conventional method, packing changes in the triangular flask, the sealing of jumping a queue, steam pressurized to 121 ℃ sterilization, pressurize 30min postcooling inserts liquid seeds and ferments to 25-28 ℃.
2: the requirement of inoculation method and inoculum concentration
A, test tube slant adopt Inoculating needle picking mycelium inoculation; Liquid seeds shaking flask inoculation kind is that cultured slant strains is extracted 1cm
2, be inoculated in 250ml or 500ml then and be equipped with in the triangular flask of fluid medium; Fermentation culture inoculation is adopted and is directly got the method that 10% shake-flask seed sterile working changes over to.
B, seed culture condition at different levels
28 ℃ of the temperature that seed culture at different levels prepare, liquid seeds are shaken bottle and are enlarged the lucifuge of vibrating in the fermentation 140rpm shaking table and cultivated 6 days.
3: the preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing. health product stock solution
A, from fermentation liquid, collect the armillaria mellea mycelium ball, reflux to boil with boiling water and carry twice, merge filtrate twice.
B, extracting solution and fermentation liquid are merged the centrifugal removal of impurity in back.Supernatant is heated to 50 ℃, and after the adding depigmenting agent stirred the 5h decolouring, centrifugal decontamination got Armillaria mella tabescens (Scop.ex Fr.) Sing. health product stock solution.
4: the preparation of health product dosage form
The preparation of a, Armillaria mella tabescens (Scop.ex Fr.) Sing. electuary
Add adjuvants such as starch 30%, dextrin 20%, cane sugar powder 10% in stock solution, behind the mixing, above-mentioned serosity is carried out spray drying, spray-dired inlet temperature is 110-150 ℃, outlet temperature 50-60 ℃.The 40-60 order that sieves, packing.
The preparation of b, Armillaria mella tabescens (Scop.ex Fr.) Sing. tablet
Armillaria mella tabescens (Scop.ex Fr.) Sing. stock solution is concentrated into 1/10, and three times of ethanol precipitations of adding 75% spend the night, collecting precipitation, and reuse acetone, ether continuous washing get exsiccant powder.Be wetting agent with 95% ethanol behind the Armillaria mella tabescens (Scop.ex Fr.) Sing. powder 40, starch 30, dextrin 20, sucrose 10 mixings, cross 20 eye mesh screens and make wet granular that being dried to moisture in 60 ℃ of baking ovens is 5%, granulate (20 order) adds 3% Pulvis Talci tabletting.
C, the capsular preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing.
Armillaria mella tabescens (Scop.ex Fr.) Sing. stock solution is concentrated into 1/10, and three times of ethanol precipitations of adding 75% spend the night, collecting precipitation, and reuse acetone, ether continuous washing get exsiccant powder.Be wetting agent behind the Armillaria mella tabescens (Scop.ex Fr.) Sing. powder 40, starch 35, dextrin 25 mixings with 95% ethanol, cross 40 eye mesh screens and make wet granular, being dried to moisture in 60 ℃ of baking ovens is 5%, and exsiccant powder is crossed the 80-120 mesh sieve and got capsule 's content, and filled capsules promptly gets the Armillaria mella tabescens (Scop.ex Fr.) Sing. capsule.
Beneficial effect of the present invention is:
1. adopt the liquid submerged fermentation method on the production technology, advantage is: the culture medium prescription cheapness, fermentation time is shortened in the growth of the suitable Armillaria mella tabescens (Scop.ex Fr.) Sing. of condition of culture greatly, and mycelia is grown fully, has improved production efficiency.
2. Du Te culture medium prescription produces great deal of bioactive substances after Armillaria mella tabescens (Scop.ex Fr.) Sing. transforms, and is more effective to improving immunity and preventing and treating chemical hepatic injury.
3. find and prove the effect that the product of Armillaria mella tabescens (Scop.ex Fr.) Sing. after fermentation has prevention and recover chemical hepatic injury first.
4. the preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing. powder makes the preparation dosage form many, convenient transportation, and long shelf-life also facilitates patients.
Embodiment
Example 1
The preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing. stock solution
1. strain adopts the strain [Armillariella tabescens (Scop.ex Fr.) Sing] at microorganism fungus kind preservation center, Beijing
2. strain inclined plane is cultivated: with the aseptic PDA of being connected to of the strain of purchasing inclined-plane, put into 28 ℃ of incubators and cultivated 10-15 days.
3. a bottle liquid seeds is shaken in preparation: extract that cultured slant strains is connected to 250ml or 500ml is equipped with in the triangular flask of fluid medium, put in 28 ℃, 140rpm shaking table the vibration lucifuge and cultivated 7 days.The aforesaid liquid seed culture medium is: murphy juice 20%, glucose 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, V
B1On a small quantity, PH 6.0.
4. Armillaria mella tabescens (Scop.ex Fr.) Sing. fermentation:, put 24 ℃, 140rpm shaking table shaken cultivation 6d with the 10% inoculum concentration inoculation that liquid seeds is pressed fermentation medium.Fermentation medium is: glucose 3%, murphy juice 14%, Semen Maydis powder 5%, bean sprout juice 3%, ammonium chloride 0.5%, potassium dihydrogen phosphate 0.05%, calcium chloride 0.3%, PH6.0.
5. from fermentation liquid, collect the armillaria mellea mycelium ball, reflux to boil with boiling water and carry twice, merge filtrate twice
6. extracting solution and fermentation liquid are merged the centrifugal removal of impurity in back.Supernatant is heated to 50 ℃, and after the adding depigmenting agent stirred the 5h decolouring, centrifugal decontamination got Armillaria mella tabescens (Scop.ex Fr.) Sing. health product stock solution.
Example 2
The preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing. electuary
Add adjuvants such as starch 20%, dextrin 20%, cane sugar powder 10% in stock solution, behind the mixing, above-mentioned serosity is carried out spray drying, spray-dired inlet temperature is 110-150 ℃, outlet temperature 50-60 ℃.The 40-60 order that sieves, packing.The key technical indexes is as follows:
Effective polysaccharide 22.1%, protein 15.6%, polypeptide 11.3%, moisture 4.3%.
Example 3
The preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing. tablet
Armillaria mella tabescens (Scop.ex Fr.) Sing. stock solution is concentrated into 1/10, and three times of ethanol precipitations of adding 75% spend the night, collecting precipitation, and reuse acetone, ether continuous washing get exsiccant powder.Be wetting agent with 95% ethanol behind the Armillaria mella tabescens (Scop.ex Fr.) Sing. powder 40, starch 30, dextrin 20, sucrose 10 mixings, cross 20 eye mesh screens and make wet granular that being dried to moisture in 60 ℃ of baking ovens is 5%, granulate (20 order) adds 3% Pulvis Talci tabletting.Every weight 0.4g.The product the key technical indexes is as follows:
Effective polysaccharide 26.5%, protein 17.8%, polypeptide 12.1%, moisture 4.1%.
Example 4
The capsular preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing.,
Armillaria mella tabescens (Scop.ex Fr.) Sing. stock solution is concentrated into 1/10, and three times of ethanol precipitations of adding 75% spend the night, collecting precipitation, and reuse acetone, ether continuous washing get exsiccant powder.Be wetting agent behind the Armillaria mella tabescens (Scop.ex Fr.) Sing. powder 40, starch 30, dextrin 20, sucrose 10 mixings with 95% ethanol, cross 40 eye mesh screens and make wet granular, being dried to moisture in 60 ℃ of baking ovens is 5%, and exsiccant powder is crossed the 80-120 mesh sieve and got capsule 's content, and filled capsules promptly gets the Armillaria mella tabescens (Scop.ex Fr.) Sing. capsule.The key technical indexes is as follows:
Effective polysaccharide 28.6%, protein 16.7%, polypeptide 11.8%, moisture 4.5%.
Zoopery of the present invention
One, animal immune experiment
Experiment material
1, strain: armillariella tabescens [Armillariella tabescens (Scop.ex Fr.) Sing] is available from BeiJing ZhongKe institute of microbiology of institute.
2, animal: mice, Cavia porcellus are provided by zoopery center, Traditional Chinese Medicine Research Institute, Sichuan Province.
Experimental technique
The phagocytic function experiment
The detection of humoral immune function (hemolysin Determination on content)
Experimental result
Influence to mice RES phagocytic function
As seen,, the Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide high dose group is compared the coefficient of engulfing of mice, and there were significant differences (P＜0.01) with normal group from table 3-1, engulfs coefficient and obviously increase, but phagocytic index and immune organ are not obviously influenced.The Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide that doses is described can strengthen mice RES phagocytic function, but is not to promote cytophagous propagation.
Table 3-1 Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide and armillarisin oral liquid are to the influence of mice RES phagocytic function
Drug dose number of animals phagocytic index is engulfed coefficient
(mg/10gd) (only) K a
Matched group-9 0.0539 ± 0.0161 6.13 ± 0.75
Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide 1.8 11 0.0692 ± 0.0232 7.18 ± 0.74
*
*P≤0.05
**P≤O.01
Table 3-2 Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide and armillarisin oral liquid are to the influence of mouse immune organ
Drug dose liver/body weight spleen/body weight
(mg/10g)
Matched group-0.05405 ± 0.0056 0.006833 ± 0.001861
Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide group 1.80 0.05059 ± 0.0062 0.006318 ± 0.002129
*P≤0.05
**P≤0.01
Influence to the generation of mice hemolytic antibody
Compare with the cyclophosphamide group, Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide has the effect of significant enhance immunity for the mice of the immunocompromised of caused by cyclophosphamide; Compare with the normal control group, Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide does not have remarkable enhancing trend (table 3-3) to the phagocytic function of normal mouse.
The influence that table 3-3 Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide and armillarisin oral liquid form the mice hemolysin
Group dosage number of animals hemolysin forms
(mg/10gd) (only) (O.D value)
Cyclophosphamide+Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide 1.8 9 0.3387 ± 0.1563
*
Cyclophosphamide group-9 0.1649 ± 0.1257
Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide group 1.8 10 0.2301 ± 0.1317
Normal control group-9 0.1948 ± 0.0987
*P≤0.05
Discuss
This experiment selects for use Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide as object of study, has measured Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide to mouse humoral immune and immune function influence.By to mice RES activate the phagocytic capacity, serum hemolysin pH-value determination pH, the result shows that the Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide vivo medicine-feeding can significantly improve the coefficient of engulfing of mice, activates the mice reticuloendothelial system, strengthens its phagocytic function; Can significantly strengthen the humoral immune function of the immunocompromised mice of caused by cyclophosphamide.
Two, animal protects the liver experiment
Experiment material
The animal mice is provided by zoopery center, Traditional Chinese Medicine Research Institute, Sichuan Province
Medicine and reagent Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide solution (low dosage 0.1g/kg; Middle dosage 0.2g/kg; High dose 0.4g/kg); 0.1% carbon tetrachloride (joining) with oil; ACP test kit (lot number 1003041)
Experimental technique
(1) mice group: 50 mices are divided into 5 groups (male and female half and half) at random, and first group is the normal control group; Second group is model group; Third and fourth, five groups be medicine feed group (solid), be divided into low dosage 0.2g/kg, middle dosage 0.4g/kg, high dose group 0.8g/kg, irritate stomach once with 0.1ml/10g every day, medicine feed is 7 days continuously; Six, seven, eight groups is administration group (liquid), is divided into low dosage 0.2g/kg, middle dosage 0.4g/kg, high dose group 0.8g/kg, and irritate stomach once with 0.1ml/10g every day, and medicine feed is 7 days continuously;
(2) moulding: irritated stomach after half an hour, dosage group and model group lumbar injection 0.1% carbon tetrachloride (0.1mg/10g) on the 7th day.
(3) second day eye socket after the moulding got blood 1-2ml, and the centrifugal 15min of 2400rpm gets serum.
(4) detect index: serum glutamic pyruvic transminase (ALT).
Experimental result
Drug dose number of animals serum glutamic pyruvic transminase (ALT)
(mg/10gd) (only) (KarU)
Matched group-10 61.5318 ± 23.4273
Model group-10 181.6650 ± 57.6920
Liquid fermentation Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide 20 10 120.3698 ± 54.0843
*
Low dose group
Liquid fermentation Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide 40 10 108.4475 ± 38.1581
*
Middle dosage group
Liquid fermentation Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide 80 7 102.5838 ± 38.2817
*
High dose group
*P≤0.05
**P≤0.01
Interpretation of result
By making liver injury model for the injected in mice carbon tetrachloride, to get blood examination at last and survey ALT, Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide group and model group compare the result and show: liquid fermentation Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide low dose group has played remarkable result to the prevention of chemical hepatic injury; Dosage group and high dose group are better than low dose group to the preventive effect of chemical hepatic injury in the liquid fermentation Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide.
The purposes of Armillaria mella tabescens (Scop.ex Fr.) Sing. health product and result of use
Armillaria mella tabescens (Scop.ex Fr.) Sing. is as a kind of medicinal fungi.It can provide the multiple nutrients material for people, in the culture fluid of its liquid fermentation and culture thing, there are a large amount of polysaccharide and protein, in addition also there are a large amount of armillarisin A, Armilariella tabescens B and abstracting, Armillaria mella tabescens (Scop.ex Fr.) Sing. third element, mannitol, and arginine, alanine, aspartic acid, threonine, glutamic acid, glycine, proline, cystine, valine, isoleucine, leucine, tyrosine, 13 seed amino acids such as serine, wherein contain eight kinds of human bodies must but can not self synthetic aminoacid, and other multiple beneficial composition, can play a part prevention and treatment to the numerous disease that causes human health to threaten.Conclude and get up to see that the Armillaria mella tabescens (Scop.ex Fr.) Sing. health product mainly contain more following to the health care of human body:
1. raising body immunity
2. chemical hepatic injury there is certain curative effect
3. radiation protection
4. treat hepatitis, gastritis, cholecystitis
Though Armillaria mella tabescens (Scop.ex Fr.) Sing. has entered the market of fungi-medicine,, also do not form bigger market, so the exploitation of Armillaria mella tabescens (Scop.ex Fr.) Sing. health product will have fine future owing to reasons such as its preparation technology and product forms.
Claims (4)
1, the method for the preparation of the strong body hepatoprotective of a kind of Armillaria mella tabescens (Scop.ex Fr.) Sing. health product stock solution and improvement Armillaria mella tabescens (Scop.ex Fr.) Sing. dosage form.Related technological process comprises:
It is characterized in that: the preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing. strains at different levels
A, strain inclined plane are cultivated: with the aseptic PDA inclined-plane that is connected to of strain, put into 28 ℃ of incubators and cultivated 10-15 days.
B, liquid seed culture medium are: murphy juice 20%, glucose 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, V
B1On a small quantity, PH6.0.Configuration according to a conventional method, packing changes in the triangular flask, the sealing of jumping a queue, steam pressurized to 121 ℃ sterilization, pressurize 30min postcooling inserts the inclined-plane seed to 25-28 ℃.
2, the method for raising Armillaria mella tabescens (Scop.ex Fr.) Sing. output according to claim 1 and improvement Armillaria mella tabescens (Scop.ex Fr.) Sing. dosage form.
It is characterized in that: the requirement of inoculation method and inoculum concentration
A, test tube slant adopt Inoculating needle picking mycelium inoculation; Liquid seeds shaking flask inoculation kind is that cultured slant strains is extracted 1cm
2, be inoculated in 250ml or 500ml then and be equipped with in the triangular flask of fluid medium; Fermentation culture inoculation is adopted and is directly got the method that 10% shake-flask seed sterile working changes over to.
B, seed culture condition at different levels
28 ℃ of the temperature that seed culture at different levels prepare, liquid seeds are shaken bottle and are enlarged the lucifuge of vibrating in the fermentation 140rpm shaking table and cultivated 6 days.
3, the preparation method of the strong body hepatoprotective health product of claim 1 and 2 described raising Armillaria mella tabescens (Scop.ex Fr.) Sing. output and improvement Armillaria mella tabescens (Scop.ex Fr.) Sing. dosage form.
It is characterized in that: the preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing. health product stock solution
A, from fermentation liquid, collect the armillaria mellea mycelium ball, reflux to boil with boiling water and carry twice, merge filtrate twice.
B, extracting solution and fermentation liquid are merged the centrifugal removal of impurity in back.Supernatant is heated to 50 ℃, and after the adding depigmenting agent stirred the 5h decolouring, centrifugal decontamination got Armillaria mella tabescens (Scop.ex Fr.) Sing. health product stock solution.
4, according to claim 1,2 and 3 described raising Armillaria mella tabescens (Scop.ex Fr.) Sing. output with improve the method for Armillaria mella tabescens (Scop.ex Fr.) Sing. dosage form.
It is characterized in that: the preparation of health product dosage form
The preparation of a, Armillaria mella tabescens (Scop.ex Fr.) Sing. electuary
Add adjuvants such as starch 30%, dextrin 20%, cane sugar powder 10% in stock solution, behind the mixing, above-mentioned serosity is carried out spray drying, spray-dired inlet temperature is 110-150 ℃, outlet temperature 50-60 ℃.The 40-60 order that sieves, packing.
The preparation of b, Armillaria mella tabescens (Scop.ex Fr.) Sing. tablet
Armillaria mella tabescens (Scop.ex Fr.) Sing. stock solution is concentrated into 1/10, and three times of ethanol precipitations of adding 75% spend the night, collecting precipitation, and reuse acetone, ether continuous washing get exsiccant powder.Be wetting agent with 95% ethanol behind the Armillaria mella tabescens (Scop.ex Fr.) Sing. powder 40, starch 30, dextrin 20, sucrose 10 mixings, cross 20 eye mesh screens and make wet granular that being dried to moisture in 60 ℃ of baking ovens is 5%, granulate (20 order) adds 3% Pulvis Talci tabletting.
C, the capsular preparation of Armillaria mella tabescens (Scop.ex Fr.) Sing.
Armillaria mella tabescens (Scop.ex Fr.) Sing. stock solution is concentrated into 1/10, and three times of ethanol precipitations of adding 75% spend the night, collecting precipitation, and reuse acetone, ether continuous washing get exsiccant powder.Be wetting agent behind the Armillaria mella tabescens (Scop.ex Fr.) Sing. powder 40, starch 35, dextrin 25 mixings with 95% ethanol, cross 40 eye mesh screens and make wet granular, being dried to moisture in 60 ℃ of baking ovens is 5%, and exsiccant powder is crossed the 80-120 mesh sieve and got capsule 's content, and filled capsules promptly gets the Armillaria mella tabescens (Scop.ex Fr.) Sing. capsule.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100224865A CN1329503C (en) | 2004-05-11 | 2004-05-11 | Healthy product raw liquor of armilariella tabescens for strengthening the body and protecting the liver and method for improving the formulation of armilariella tabescens |
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|---|---|---|---|
| CNB2004100224865A CN1329503C (en) | 2004-05-11 | 2004-05-11 | Healthy product raw liquor of armilariella tabescens for strengthening the body and protecting the liver and method for improving the formulation of armilariella tabescens |
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| CN1329503C CN1329503C (en) | 2007-08-01 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895292B (en) * | 2006-06-28 | 2011-09-07 | 四川隆盛药业有限责任公司 | Leukothrix capsule medicine, its discrimination and quality standard inspection |
| CN102353675A (en) * | 2006-06-28 | 2012-02-15 | 四川隆盛药业有限责任公司 | Identification method of Armillaria tabescens capsule medicament |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031915C (en) * | 1992-09-08 | 1996-06-05 | 淮南市科技实验厂 | Method for producing "Liangjun" mushroom syrup |
| CN1066331C (en) * | 1997-11-10 | 2001-05-30 | 王文献 | Oral medicinal liquor prepared from Armilariella tabescens |
-
2004
- 2004-05-11 CN CNB2004100224865A patent/CN1329503C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895292B (en) * | 2006-06-28 | 2011-09-07 | 四川隆盛药业有限责任公司 | Leukothrix capsule medicine, its discrimination and quality standard inspection |
| CN102353675A (en) * | 2006-06-28 | 2012-02-15 | 四川隆盛药业有限责任公司 | Identification method of Armillaria tabescens capsule medicament |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1329503C (en) | 2007-08-01 |
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