CN1557425A - Nanometer Chinese traditional medicine for treating osteoporosis - Google Patents
Nanometer Chinese traditional medicine for treating osteoporosis Download PDFInfo
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Abstract
The present invention is one kind of nano Chinese medicine for preventing and treating osteoporosis, and belongs to the field of biological pharmacology. The medicine recipe consists of oyster, active pilose antler, prepared rhizome of rehmannia, kudzu vine root, eucommia bark, ginseng and tortoise plastron. The preparation process includes nano technological process to prepare oyster calcium, freeze drying to prepare active pilose antler, crushing ginseng into fine powder, alcohol extracting eucommia bark, merging water decoction of tortoise plastron, prepared rhizome of rehmannia and kudzu vine root, decompression evaporation to obtain fine powder, mixing and pelletizing to form the medicine. The medicine has the advantages of benefiting vital energy and life essence, strengthening kidney, replenishing calcium, etc. and has obvious osteoporosis preventing and treating effect.
Description
Technical field: the nanometer Chinese medicine that the present invention relates to a kind of protect against osteoporosis in the field of biological pharmacy.
Background technology: osteoporosis is common, the frequently-occurring disease of old people, needs the crowd of protect against osteoporosis, more than 100,000,000 people, and the trend that increases is year by year arranged.Along with the aging increased population, the prevalence of osteoporosis and the incidence rate of complication thereof also constantly increase; Fracture and complication thereof have become life of elderly person downgrade and dead important diseases due to the osteoporosis.The known primary osteoporosis cause of disease and endocrine factors, calcium balance are lacked of proper care closely related.External research recently relates to receptor (estrogen receptor, PTH receptor, CT receptor etc.), gene (VDR allele, female hormone receptor gene etc.) and influences bone formation and the relevant factor of bone resorption, trace element variation etc.At present, the calsium supplement of treatment osteoporosis is in vogue, as huge can calcium, calcium etc. in the calcium, but the key issue that the calcium preparation treatment exists is the ionizing problem of calcium, with the absorption of calcium, human body availability and closely related in skeleton deposition etc.Nanotechnology is the high-and-new science and technology that forms the beginning of the eighties, and the application of nanotechnology in biomedical engineering more and more widely.Nanometer level bioactive macromolecule particle has good adsorptivity, guidance quality, permeability, stationarity, be absorbed by the body as the effective ingredient of nano calcium lactate oral administration 98%, and existing calcium preparation can only be absorption of human body 30%.The Chinese medicine nanometer calcium is not seen in report as yet.Utilize high adsorption, guidance quality, the stationarity of nanometer Chinese medicine calcium, combine with kidney-nourishing tcm drug, according to the theory of Chinese medical science of " kidney governing bones ", enriching kidney essence, calcium-tonifying bone-strengthening will become the protect against osteoporosis effective way.Therefore, develop a kind of nanometer Chinese medicine of protect against osteoporosis, solve problems such as the drug absorption of treatment osteoporosis at present and bioavailability be relatively poor, be new problem anxious to be solved both at home and abroad always.
Summary of the invention: the object of the present invention is achieved like this: its formula for a product is Concha Ostreae 5-10%, active hairy antler 5-10%, Radix Rehmanniae Preparata 15-20%, Radix Puerariae 15-20%, Cortex Eucommiae 15-20% Radix Ginseng 15-20%, Carapax et Plastrum Testudinis 15-20%; Preparation technology is, Concha Ostreae cleaned, and through the alligator coarse crushing, bolting, the impurity of skimming freezes through the ultralow temperature liquid nitrogen deep, and shakes or the high energy ball mill ball milling with High-frequency machine, and its particle diameter is reached below the 200nm, makes nanometer Concha Ostreae calcium; Active hairy antler is the aseptic fresh Cornu Cervi Pantotrichum of taking, liquid nitrogen freezing immediately, and-70 ℃ of preservations cut into thin slice during pharmacy, pulverize after lyophilization; Get the Radix Ginseng powder and be broken into fine powder; The Cortex Eucommiae merges 2 times extracting solution with 70% alcohol reflux 2 times, filters, and reclaims ethanol, the simmer down to clear paste; Carapax et Plastrum Testudinis decocts with water 2 hours, filters, and medicinal residues add Cortex Eucommiae medicinal residues, Radix Rehmanniae Preparata, Radix Puerariae, decoct with water 2 times, merge decocting liquid, filter, filtrate and Carapax et Plastrum Testudinis filtrate merge, reduction vaporization, simmer down to clear paste mix with Cortex Eucommiae clear paste, admix Radix Ginseng, active hairy antler fine powder, drying under reduced pressure, with the solid coarse powder, enter the supersonic airstream mill again, the micropowder mean diameter is reached about 1000 nm; At last, nanometer Concha Ostreae calcium is mixed with homogenizer fully with the Chinese medicine micropowder, the adding dextrin is an amount of, makes granule with ethanol, and drying forms.Main points of the present invention are its formula for a product and processing technology.Its technology preparation principle is, theory of Chinese medical science according to " kidney storing essence master bone ", " gas energy spermatogenesis ", utilize high adsorption, guidance quality, the stationarity of nanometer Chinese medicine calcium, reasonable compatibility with vital energy benefiting and the kidney invigorating Chinese medicine, changed the conventional medicament weak point, strengthen the absorption and the bioavailability of medicine, promoted calcium and the deposition of trace element in osseous tissue; Use nanometer Chinese medicine simultaneously, increase the targeting of medicine, the kidney tonifying calcium-tonifying bone-strengthening is more conducive to medicine and plays a role.Patent of the present invention provides a kind of nanometer Chinese medicine compound, take QI invigorating spermatogenesis, kidney tonifying to fill out marrow, calcium-tonifying bone-strengthening method of treatment, select the marine organisms Concha Ostreae that contains the polycalcium composition for use, the development of applying nano technology becomes nanometer Chinese medicine calcium, kidney-nourishing tcm drug micropowder and its is synthetic, to improve curative effect, will become the protect against osteoporosis effective way.By the development and use living marine resources, have good economic benefits and application prospect; Simultaneously, promote senior health and fitness's existence, life lengthening, have social benefit widely.Monarch drug is filled out marrow with the active hairy antler the kidney invigorating in the side, bone and muscle strengthening, and Compendium of Material Medica is discussed its effect " spermatogenesis is mended marrow, the Yiyang of nourishing blood, strengthening the tendons and bones ", so neck first of the side of being.Radix Ginseng, Radix Rehmanniae Preparata, the Cortex Eucommiae are ministerial drug, and Radix Ginseng is the key medicine of QI invigorating, and in order to do its QI invigorating spermatogenesis, tonifying five ZANG-organs six internal organs, plenty of vital essence in five ZANG-organs circulating normally are " smart spontaneous and shape is contained certainly " then; Radix Rehmanniae Preparata is the key medicine of the kidney invigorating, kidney nourishing water, the gas of enriching blood, replenishing essence marrow; The little suffering of Cortex Eucommiae sweet in the mouth, " Gan Wenneng mend, little suffering can be moistened ", the strengthening bone and muscle with invigorating the liver and kidney controls that the waist knee joint is weak aches.Radix Puerariae, Carapax et Plastrum Testudinis are adjuvant drug, and Radix Puerariae inspires gastric qi up, can delay spasm of nape and back pain again, and modern pharmacology experiment showed, that isoflavone like substance can effectively be treated osteoporosis in the Radix Puerariae; The Carapax et Plastrum Testudinis replenishing kidney and strengthening bone is apt to control suffer from a deficiency of the kidney " the waist foot is ached ", and because of its nourishing YIN for suppressing the hyperactive YANG, and YANG invigorating such as anti-Cornu Cervi Pantotrichum, the Cortex Eucommiae are partially.Concha Ostreae is a messenger drug, goes into kidney meridian, and its chemical constituent contains calcium carbonate, calcium phosphate, calcium sulfate, and trace element such as cupric, ferrum, zinc, manganese, strontium, chromium, and kidney tonifying is replenished the calcium, and makes nanometer Chinese medicine, strengthens its guidance quality, high adsorption, stationarity.All medicines share, and play the effect that QI invigorating spermatogenesis, kidney tonifying are filled out marrow, calcium-tonifying bone-strengthening altogether.In addition, be hot-working to the traditional concocting method of Cornu Cervi Pantotrichum, but most of heat labile active component may be therefore destroyed.We are that the pilose antler active composition that freeze drying technology is processed is studied to active hairy antler, find that it has the effect of good protect against osteoporosis really.Using method is, and is oral, each 15 grams, and every day 2 times, 3 months is 1 course of treatment.A kind of nanometer Chinese medicine of protect against osteoporosis compared with prior art, have design science novelty, original creation preparation technology, crude drug source extensively, found QI invigorating spermatogenesis, kidney tonifying fill out marrow, calcium-tonifying bone-strengthening method of treatment and replenish the calcium, the advantage of the organic compatibility of kidney-tonifying preparation, both the functional activity of enhancing body was on the whole adjusted the endocrine disturbance; From bone reconstruction aspect, adjust disorder of calcium metabolism again, the protect against osteoporosis effect is remarkable, will be widely used in protect against osteoporosis in the field of biological pharmacy.
The specific embodiment:
Embodiments of the invention are as follows, with Concha Ostreae 500g through the alligator coarse crushing, bolting, the impurity of skimming freezes through the ultralow temperature liquid nitrogen deep, and shakes or the high energy ball mill ball milling with High-frequency machine, and its particle diameter is reached below the 200nm, makes nanometer Concha Ostreae calcium; Active hairy antler 500g is the aseptic fresh Cornu Cervi Pantotrichum of taking, liquid nitrogen freezing immediately, and-70 ℃ of preservations cut into thin slice during pharmacy, pulverize after lyophilization; Get Radix Ginseng 750g and be ground into fine powder; Cortex Eucommiae 750g merges 2 times extracting solution with 70% alcohol reflux 2 times, filters, and reclaims ethanol, the simmer down to clear paste; Carapax et Plastrum Testudinis 750g decocts with water 2 hours, filters, and medicinal residues add Cortex Eucommiae medicinal residues, Radix Rehmanniae Preparata 750g, Radix Puerariae 750g, decoct with water 2 times, merge decocting liquid, filter, filtrate and Carapax et Plastrum Testudinis filtrate merge, reduction vaporization, simmer down to clear paste mix with Cortex Eucommiae clear paste, admix Radix Ginseng, active hairy antler fine powder, drying under reduced pressure, with the solid coarse powder, enter the supersonic airstream mill again, the micropowder mean diameter is reached about 1000nm; At last, nanometer Concha Ostreae calcium is mixed with homogenizer fully with the Chinese medicine micropowder, the adding dextrin is an amount of, makes granule with ethanol, and drying forms.
The zoopery report:
Materials and methods: laboratory animal is cleaning level 120 of female sd inbred rats (body weight 260-300g, 4.5 monthly ages, not copulation), purchases in west, Shanghai pul-must triumphant Experimental Animal Center (Shanghai is moving closes the card word No. 152).After animal was bought, raising in ambient temperature was 20 ± 1 ℃ SPF level laboratory, and the aseptic full-valence pellet feed of ad lib rat (people's feed factory before Shenyang City's Yuhong District) is drunk the tap water behind the autoclave sterilization.By body weight random packet, 15 of normal group, surplus person carries out modeling and handles.
The bilateral ovaries extraction method is adopted in the foundation of osteoporosis rat model.Postoperative 7 days is divided into 5 groups (15 every group) with oophorectomize group survivor at random by body weight, i.e. dosage group in the blank group of model, the heavy dose of group of nanometer Chinese medicine compound, the nanometer Chinese medicine compound, nanometer Chinese medicine compound small dose group, GUSHUKANG KELI matched group, calcium and strange D matched group, estradiol (E2) matched group.
Postoperative beginning in 7 days administration: dosage is dosage group 6g/kg body weight/d, nanometer Chinese medicine compound small dose group 3g/kg body weight/d in the heavy dose of group of nanometer Chinese medicine compound 9g/kg body weight/d, the nanometer Chinese medicine compound; GUSHUKANG KELIJI (Kang Chen of Donggang City pharmaceutical Co. Ltd) 6g/kg body weight/d, calcium and strange D matched group (Hui Shi-hundred palace pharmaceutical Co. Ltd) 120mg/kg body weight/d, E2 matched group (French Schering Corp) 0.1mg/kg body weight/d, irritate stomach, each organizes the administration volume is the 1ml/100g body weight, every day 1 time.Normal group, model blank are organized during the not perfusion, irritate the isopyknic normal saline of stomach every day, every morning administration, 12 weeks of successive administration.Surviving animals after the administration 24 hours is the last time put to death and is gathered following specimen and carries out determination and analysis.
Result and analysis:
1, the Osteoporosis Rats model is set up.Model evaluation: (1) uterus index obviously reduces; (2) stripped femoral bmd significantly descends; (3) biochemical markers of bone metabolism, bone formation index Bone Gla protein, the anti-tartaic acid phosphatase of bone resorption marker etc. have typical osteoporosis performance.Data see the following form respectively.
2, the nanometer Chinese medicine compound can obviously improve bone density.See Table 1.
Table 1 is respectively organized medicine to the influence of the osteoporosis rat bone density of the suffering from a deficiency of the kidney (n=15 of x ± s)
Group femoral bmd femoral head 1/3 bone density
Normal 0.183 ± 0.014*, 0.189 ± 0.014*
The blank group of model 0.124 ± 0.005# 0.128 ± 0.006#
Chinese medicine compound (heavy dose) 0.175 ± 0.112* 0.186 ± 0.008*
Chinese medicine compound (middle dosage) 0.173 ± 0.009*, 0.183 ± 0.007*
Chinese medicine compound (low dose) 0.176 ± 0.009* 0.185 ± 0.009*
Gaierqi D (vitamin D3 and calcium carbonate) 0.175 ± 0.010* 0.184 ± 0.007*
E2 0.177±0.008* 0.184±0.010*
GUSHUKANG 0.161 ± 0.010* 0.175 ± 0.009*
Annotate: the empty group of * and mould compares p＜0.05 # and normal group compares p＜0.05
3, the nanometer Chinese medicine compound can obviously be improved bone metabolism.Improve bone formation index serum osteocalcin content, reduce the anti-tartaic acid phosphatase of bone resorption marker serum content, see Table 2, table 3.
Table 2 is respectively organized medicine to the influence of osteoporosis rat serum osteocalcin content (x ± s)
Group serum osteocalcin content (ng/ml)
Normal group 865.000 ± 221.877*
The blank group of model 525.000 ± 177.204#
Chinese medicine compound (heavy dose) group 573.250 ± 236.356*
Chinese medicine compound (middle dosage) group 652.750 ± 222.218*
Chinese medicine compound (low dose) group 645.750 ± 187.435*
Gaierqi D (vitamin D3 and calcium carbonate) group 622.250 ± 189.573*
E2 organizes 633.500 ± 139.867*
GUSHUKANG group 618.750 ± 132.362*
The empty group of notes: * and mould relatively P＜0.05 compares P＜0.05 with normal group
Table 3 is respectively organized medicine to the influence of the anti-tartaic acid phosphatase of osteoporosis rat serum (x ± s)
The anti-tartaic acid phosphatase of group serum (u/l)
Normal group 6.992 ± 1.859*
The blank group of model 17.656 ± 4.647#
Chinese medicine compound (heavy dose) group 11.423 ± 3.725*
Chinese medicine compound (middle dosage) group 14.285 ± 4.377*
Chinese medicine compound (low dose) group 12.341 ± 4.101*
Gaierqi D (vitamin D3 and calcium carbonate) group 12.392 ± 3.577*
E2 organizes 13.123 ± 5.570*
GUSHUKANG group 11.106 ± 4.275*
The empty group of notes: * and mould relatively P＜0.05 compares P＜0.05 with normal group
4, the nanometer Chinese medicine compound can increase the uterus index.But difference is not obvious, sees Table 4.
Table 4 is respectively organized medicine to the exponential influence in osteoporosis rat uterus of suffering from a deficiency of the kidney (x ± s)
Group uterus index (mg/g)
Normal group 1.793 ± 0.431*
The blank group of model 0.328 ± 0.222#
Chinese medicine compound (heavy dose) 0.414 ± 0.098
Chinese medicine compound (middle dosage) 0.377 ± 0.091#
Chinese medicine compound (low dose) 0.376 ± 0.136#
Gaierqi D (vitamin D3 and calcium carbonate) 0.272 ± 0.052
E2 0.510±0.774#
GUSHUKANG 0.347 ± 0.118#
Annotate: the empty group of * and mould compares p＜0.05 # and normal group compares p＜0.05
The nanometer Chinese medicine compound has and improves bone density, improves bone metabolism, promotes bone formation, suppresses effect such as bone resorption, thereby osteoporosis is had good prevention effect.
Claims (1)
1, a kind of nanometer Chinese medicine of protect against osteoporosis is characterized in that: its formula for a product is Concha Ostreae 5-10%, active hairy antler 5-10%, Radix Rehmanniae Preparata 15-20%, Radix Puerariae 15-20%, Cortex Eucommiae 15-20%, Radix Ginseng 15-20%, Carapax et Plastrum Testudinis 15-20%; Preparation technology is, with Concha Ostreae through the alligator coarse crushing, bolting, the impurity of skimming freezes through the ultralow temperature liquid nitrogen deep, and shakes or the high energy ball mill ball milling with High-frequency machine, and its particle diameter is reached below the 200nm, makes nanometer Concha Ostreae calcium; Active hairy antler is the aseptic fresh Cornu Cervi Pantotrichum of taking, liquid nitrogen freezing immediately, and-70 ℃ of preservations cut into thin slice during pharmacy, pulverize after lyophilization; Get the Radix Ginseng powder and be broken into fine powder; The Cortex Eucommiae merges 2 times extracting solution with 70% alcohol reflux 2 times, filters, and reclaims ethanol, the simmer down to clear paste; Carapax et Plastrum Testudinis decocts with water 2 hours, filters, and medicinal residues add Cortex Eucommiae medicinal residues, Radix Rehmanniae Preparata, Radix Puerariae, decoct with water 2 times, merge decocting liquid, filter, filtrate and Carapax et Plastrum Testudinis filtrate merge, reduction vaporization, simmer down to clear paste mix with Cortex Eucommiae clear paste, admix Radix Ginseng, active hairy antler fine powder, drying under reduced pressure, with the solid coarse powder, enter the supersonic airstream mill again, the micropowder mean diameter is reached about 1000nm; At last, nanometer Concha Ostreae calcium is mixed with homogenizer fully with the Chinese medicine micropowder, the adding dextrin is an amount of, makes granule with ethanol, and drying forms.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100211333A CN100391497C (en) | 2004-02-11 | 2004-02-11 | Nanometer Chinese traditional medicine for treating osteoporosis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104434977A (en) * | 2014-12-03 | 2015-03-25 | 吉林省东鳌鹿业集团有限公司 | Preparation method of calcium supplement product |
| CN106434089A (en) * | 2016-10-12 | 2017-02-22 | 烟台市昊星晟信息技术有限公司 | Preparation method of health-care wine |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1051471C (en) * | 1997-09-29 | 2000-04-19 | 苏顺兴 | Drug composition for curing osteoporosis |
| CN1242774C (en) * | 2001-10-17 | 2006-02-22 | 宋大文 | Medicine for curing proliferation arthritis of spine and its preparation method |
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- 2004-02-11 CN CNB2004100211333A patent/CN100391497C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104434977A (en) * | 2014-12-03 | 2015-03-25 | 吉林省东鳌鹿业集团有限公司 | Preparation method of calcium supplement product |
| CN106434089A (en) * | 2016-10-12 | 2017-02-22 | 烟台市昊星晟信息技术有限公司 | Preparation method of health-care wine |
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| CN100391497C (en) | 2008-06-04 |
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