CN1557281A - Skin apolexis preventing delaying polypeptide and compositions - Google Patents
Skin apolexis preventing delaying polypeptide and compositions Download PDFInfo
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- CN1557281A CN1557281A CNA2004100161086A CN200410016108A CN1557281A CN 1557281 A CN1557281 A CN 1557281A CN A2004100161086 A CNA2004100161086 A CN A2004100161086A CN 200410016108 A CN200410016108 A CN 200410016108A CN 1557281 A CN1557281 A CN 1557281A
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Abstract
The present invention relates to polypeptide and its composition for preventing and delaying skin senility, and is especially the process of preparing polypeptide and its composition from milk and relevant material for preventing and delaying skin senility. The present invention features that ultrafiltering membrane or cationic exchange resin is used in extracting and separating natural polypeptide and its composition from cow colostrums, milk or whey; and/or protein decomposing enzyme is used to hydrolyze milk protein to prepare polypeptide and its composition. The product is applied in various cosmetic products for preventing and delaying skin senility.
Description
Affiliated technical field
The present invention relates to the polypeptide and the compositions of a kind of prevention and delaying decrepitude of skin, relate in particular to a kind of polypeptide and compositions of from milk and related raw material, preparing, be used for preventing method with delaying decrepitude of skin.
The invention technical background
As everyone knows, in the milk except containing the multiple nutrients material, also contain the active substance that can promote the cell growth, in milk, find structurally relevant insulin like growth factor (IGF-I and II) and cattle colostrums somatomedin (BCGF) with platelet derived growth factor (PDGF).
In the world patent application (WO 97/16460), disclosing milk has the promotion growth activity to rat breast and fibroblast (Rama 27), but this cell line is not significantly by insulin like growth factor (IGF) or platelet derived growth factor (PDGF) institute stimulating growth.
Zoopery confirms, cause this promotion growth activity the effect of similar epidermis biotic factor (EGF) sample be certain a series of polypeptide, the similar of these sequences polypeptides is in α-S
2The structure of casein precursor C-terminal.
The extract that world patent application (WO 91/00994, and WO 95/29933, and WO 96/34614) discloses milk is as the preparation of growth promoter and the damage of treatment digestive tract, but do not comprise being specifically designed to and promote skin growth and slow down aging.
The zoopery of many tame foreign studies mechanism confirms to have the promotion cells growth activity, the polypeptide and the compositions of prevention and delaying decrepitude of skin, similar and α-S
2The correlation type of casein precursor, these amino acid sequence of polypeptide contain three or more aminoacid, and are present in the milk with free state, also can obtain by the proteolytic digestion of milk protein.Amino acid whose number is not particularly limited in this peptide species and the compositions, as long as three or more aminoacid is arranged, but do not comprise casein N-terminal structure, amino acid whose number is 3～50 in the polypeptide, 4～50,5～50, between 6～50, these polypeptide may be by stimulating growth of fibroblasts, realizing the function of its anti aging effect.
Technical problem to be solved by this invention is that preparation has the growth of the cell of promotion from milk or related raw material, to the polypeptide and the compositions of anti aging effect, and uses in the various beauty and make-up products.
Summary of the invention
The technical scheme that technical solution problem of the present invention is adopted, it is characterized in that, adopt supermicro filtration membrane or the cation exchange resin mode is extracted, separating natural polypeptide and compositions from cattle colostrums, milk or milk surum, adopt protease from milk protein, to prepare polypeptide and compositions by the Proteolytic enzyme mode, natural polypeptides or protolysate independent or the use in conjunction separation and Extraction prepare polypeptide, be applied in the various beauty and make-up products prevention and delaying decrepitude of skin.
Technical characterictic of the present invention is, can adopt the ultrafiltration mode from cattle colostrums, extract natural polypeptides and compositions in milk or the milk surum to anti aging effect, its method is, use the defat cattle colostrums, skim milk or milk surum, adopting molecular weight is 8,000～15, the supermicro filtration membrane of 000D, preferably molecular weight is 10, the supermicro filtration membrane of 000D, use common ultrafiltration apparatus, preferably staggered tangent plane fluid ultrafiltration apparatus, separating and filtering, collect filtering supernatant, the reuse molecular weight is 1, the 000D ultrafilter membrane is concentrated into 20%～30% of start volume, and it is standby to adopt vacuum drying or cryodesiccated mode to be dried to powder, includes abundant polypeptide and compositions to anti aging effect.
Technical characterictic of the present invention is, the mode that also can adopt free polypeptide of cationic exchange resin adsorption and compositions is from cattle colostrums, preparation is to the natural polypeptides and the compositions of anti aging effect in milk and the milk surum, its method is, use the defat cattle colostrums, skim milk, milk surum is separated macromolecular substances through the micro-filtrate membrane filtration of 0.1～10.mu.m, can adopt the cation exchange resin of any kind, but preferably select for use average particulate diameter to surpass 100.mu.m, and have a cation exchange resin that enough mechanical strengths are resisted the high flow rate pressure type, should adopt PH is that 5.5～7.5 phosphate buffer comes the pre-equilibration chromatographic column, operating temperature is preferably lower than 10 ℃, to reduce microbial reproduction, crossing the post surface area should be above 500cm/ hour, liquid cross the post rate should 100～600 capacity/hour, after crossing post, with PH is 5.5～7.5, concentration is the following sodium chloride flushing of 0.2mol, it is 5.5～7.5 that eluent should adopt PH, concentration is the sodium chloride solution eluting of 0.25～0.5mol, the eluent molecular weight is 1, after the ultrafilter membrane of 000D is concentrated into initial volume 20%～30%, vacuum drying or lyophilization become powder, include abundant polypeptide and compositions to anti aging effect.
Technical characterictic of the present invention is, preparing the present invention by the Proteolytic enzyme mode is milk protein to the raw material of anti aging effect polypeptide and compositions, comprise the albumen in the milk such as casein, beta lactoglobulin, alpha-lactalbumin, can adopt liquid milk raw materials such as milk, cattle colostrums is clear, also can adopt casoid flour, serum egg albumen powder or serum albumen to concentrate albumen dry powder such as powder as raw material.If select for use albumen dry powder as raw material, casein can be selected acid casein or fat-free milk solids (NFDM) for use, lactalbumin can be selected the milk surum that obtains in the preparation of cheese process for use, especially the dry powder of sweet whey preparation or the protein concentrate that obtains through ultrafiltration, lactalbumin can also be selected for use through ion exchange or electrodialysis and the lactalbumin of demineralization, also can select for use the cheese hydrochlorate for example cheese acid sodium, dried potassium caseinate or industrial casein as raw material.
Technical characterictic of the present invention is, if adopt milk or cattle colostrums as raw material, need be with the centrifugation defat, adopting ultrafiltration apparatus simultaneously is 30 with molecular weight, 000～40, small-molecule substances such as the ultrafilter membrane of 000D demineralizes, lactose, and condensing protein to 10～60 gram albumen/liter, 40～60 gram albumen/rise concentration preferably; If with albumen dry powder or protein liquid concentrated solution as raw material, albumen can dilute or restore into about 10～60 gram albumen/liter, preferably 40～60 gram albumen/liter solution, wherein casein and whey protein proportions are 9: 2～2: 8, preferably 8: 2～6: 4; If the mixture that adopts trypsin and chymase is as protease, tryptic activity is 800～1800USP unit/milligram in this mixture, preferably be equal to or greater than 1800USP unit/milligram, the activity of chymase is 150～350USP unit/milligram, preferably be equal to or greater than 350USP unit/milligram, trypsin is 1～18 with the ratio of chymase, preferably 4～6, the percentage by weight of enzymatic mixture and hydrolyzed protein total amount is 0.2～1.4%, preferably 0.6～0.8% (percentage by weight).Proteoclastic program and method are, protein solution is regulated PH to 6.5～6.8, be heated to 75 ℃～85 ℃ and carried out preheating in about 10～30 minutes, make albumin (BSA) and immunoglobulin (Ig) degeneration, be cooled to the mixture that slowly adds trypsin and chymase after 30 ℃～50 ℃, pass through high pressure, the abundant homogenizing of short time, casein and lactalbumin are well-dispersed in the solution, reach fully and contact with chymase with trypsin, thereby make enzyme digestion reaction carry out more completely, the homogenizing operating pressure is 8～15Mpa, 30 ℃～50 ℃ of hydrolysis temperatures, enzymolysis time continues 4～8 hours, and pH value should maintain in 6.5～8 scopes during enzymolysis, when enzymolysis process finishes, should carry out the step of enzyme deactivation by the inactivator activity, can make enzyme deactivation by heat treatment, heating-up temperature to 85 ℃～95 ℃ was kept 10～15 minutes, made enzyme deactivation.Collecting protein hydrolyzate, is 10 with molecular weight, and the ultrafilter membrane of 000D carries out isolated by filtration, to remove not the fully macro-molecular protein of hydrolysis, be 1 with molecular weight at last, after the supermicro filtration membrane of 000D concentrates, spraying drying powder-forming includes abundant anti aging effect polypeptide.
Technical characterictic of the present invention is, if with the lactalbumin is raw material, comprise lactalbumin powder, whey protein concentrate powder etc., should adding distil water be deployed into 2%～8% protein solution, 4%～6% protein solution (40 grams～60 gram protein/rise solution) preferably, can heat to the dissolving of 40 ℃～50 ℃ help protein in allotment protein solution process, the protein solution of allotment is used for the Proteolytic enzyme process; If with the cheese hydrochlorate, for example cheese acid sodium, dried potassium caseinate are raw material, should adding distil water be deployed into 3% cheese acid salt solution, add 20ml Chymosin (rennet) in per 100 liters of cheese acid salt solutions, under 30 ℃～45 ℃ temperature, kept 40～60 minutes, add sour agent and transfer PH to 4.6～4.8, be heated to 30 ℃～50 ℃ and kept 20～40 minutes, centrifugation or filter type are collected the casein that solidifies, and prepare further to carry out the Proteolytic enzyme program.
Technical characterictic of the present invention is, collect the lactoalbumin soln or the casein solution of having allocated, inventory is dissolved in 50 ℃～85 ℃ the distilled water, add alkali liquor and transfer PH to 7～9, homogenizing operating pressure 8～15Mpa high pressure homogenize becomes the pasty state base material, presses enzyme concentration 0.08%～1.5%, preferably 0.4%～0.6% drops into trypsin, transfer pH value to 3～6, kept 2～8 hours under 37 ℃～54 ℃ temperature are bathed, caseinhydrolysate is after enzymolysis process is finished, heat rapidly to 10 minutes deactivation trypsin of 85 ℃ of insulations, protein hydrolyzate is 5,000～15 by molecular weight, the ultrafilter membrane of 000D, separate the not high molecular weight protein of abundant hydrolysis, collecting ultrafiltration supernatant liquid, is 1,000～5 with molecular weight, 000D, preferably 2,000～3, after the ultrafilter membrane of 000D concentrates, spray drying or vacuum drying become powder, include the polypeptide and the compositions of abundant anti aging effect.
Technical characterictic of the present invention is, in order to make the Proteolytic enzyme process abundant, and guarantee that most of protein chains rupture, a kind of mode of improvement can be, after Proteolytic enzyme carries out one hour, continue with staggered tangent plane fluid ultrafiltration apparatus, adopting molecular weight is 8,000～12, the ultrafilter membrane of 000D continues isolated by filtration, molecular weight continues to be collected less than the micromolecule polypeptide in ultrafilter membrane aperture, and molecular weight greater than the ultrafilter membrane aperture not fully the protein molecule of hydrolysis sent back to again and continued hydrolysis in the proteolysis reaction pot, this method not only makes the micromolecule polypeptide that has been hydrolyzed continue to be collected, and can also promote the cross reaction contact between albumen and the enzyme, makes Proteolytic enzyme more thorough.
Technical characterictic of the present invention is, the polypeptide of the anti aging effect of the inventive method preparation and compositions have the effect to anti aging effect, comprise that prevention and delaying skin are loose, skin corrugates, increase skin elasticity, promote promoting epidermization, its effect to anti aging effect passes through to stimulate fibroblastic growth, promote the synthetic and secretion of collagen, stimulate the growth of keratinocyte, have and the similar effect of fibroblast growth factor (EGF), owing to do not contain protein, so they can sensitization, and product belongs to all-natural product, and life-time service side effect can not occur.
Technical characterictic of the present invention is, natural polypeptides that the present invention extracts from cattle colostrums, milk and milk surum and compositions and the polypeptide and the compositions that prepare by Proteolytic enzyme, can use separately or use in conjunction, use in conjunction may be a better choice, the anti aging effect polypeptide of the inventive method preparation and compositions can be used as additive add in all kinds of cosmetics and cosmetics in, include but not limited to protective skin cream, surfactant, cold cream, day cream, late frost, cleansing milk, skin protection oil, cold cream etc., help user prevention and delaying decrepitude of skin.
The invention has the beneficial effects as follows that prepare polypeptide and the compositions that clear and definite defying age effect is arranged with natural material, life-time service safety has no side effect, and effectively helps user to anti aging effect.
The specific embodiment
The invention will be further described below in conjunction with embodiment:
Example 1
The polypeptide and the compositions of preparation anti aging effect from milk
Collect 100 liters of fresh milks, with the centrifugation defat, adopting molecular weight is 10, and the supermicro filtration membrane of 000D is used staggered tangent plane fluid ultrafiltration apparatus (U.S. Pall company produces the TFF device) separating and filtering, the same equipment of collecting of supernatant suction, with molecular weight is 1, after the ultrafilter membrane of 000D is concentrated into start volume 20%, with 60 ℃ of vacuum dryings, preparation dry powder includes abundant polypeptide and compositions to anti aging effect.
Collect filtering first ultrafiltration and concentration liquid, with vacuum pump or the extremely staggered tangent plane fluid ultrafiltration apparatus of peristaltic pump suction, with molecular weight is 40, the ultrafilter membrane of 000D separates small molecular weight material, and condensing protein to 50 grams per liter, pump is taken out protein solution to reaction pot, heat to keep and made the immunoglobulin degeneration in 10 minutes to 85 ℃, be cooled to 50 ℃, add trypsin and chymotrypsin protein enzymatic mixture, in an amount equivalent to 0.8% (percentage by weight) of hydrolyzed protein amount (trypsin wherein: chymase=5: 1, tryptic activity is equal to or greater than 1800USP unit/milligram, chymotrypsin activity is equal to or greater than 350USP unit/milligram), adjust PH to 6.8, holding temperature is at 50 ℃, continue 6 hours, high pressure homogenize, the homogenizing operating pressure is 10MPa, and enzymolysis process finishes, and heats to keep to 95 ℃ to make enzyme-deactivating in 10 minutes, take out protein hydrolyzate with vacuum pump and enter staggered tangent plane fluid ultrafiltration apparatus, adopting molecular weight is 10, and the ultrafilter membrane of 000D separates unhydrolysed macro-molecular protein, collects ultrafiltration supernatant liquid, directly spray drying prepares dry powder, includes abundant antidotal polypeptide and compositions.
Embodiment 2
With the casein is the polypeptide and the compositions of feedstock production anti aging effect
With 100 kilograms of cheese acid sodium is raw material, cheese acid sodium solution with dissolved in distilled water allotment 3%, add Chymosin 20ml/100 and rise solution, transfer PH to 6.8, be warming up to 37 ℃ and kept 50 minutes, add 4N HCL and transfer PH to 4.6, be heated to 40 ℃ and kept 20 minutes, with the speed of 5000 rev/mins in centrifuge centrifugal 15 minutes, precipitate agglutinative casein.
In being 6.2% casein solution, concentration drops into 50 ℃ distilled water, add the 2N potassium hydroxide and transfer PH to 8, the homogenizing operating pressure is 12Mpa, enzyme concentration is 4 gram/L (weight/volume percentage ratios), add trypsin Sigma Pancretin ofbovin origin having an activity 4 NF), PH maintains PH about 8 with the potassium hydroxide of 2N in the reaction pot, temperature maintenance is at 37 ℃～40 ℃, continue 6 hours, began back one hour at enzymolysis, protein hydrolyzate begins that (the ultrafilter membrane molecular weight is 10 at staggered tangent plane fluid ultrafiltration apparatus, 000D) and between the enzyme digestion reaction pot circulate repeatedly, collect the micromolecule polypeptide of osmotic ultrafiltration film, the not hydrolyzed protein of macromolecule is sent into enzymolysis in the reaction pot again, enzymolysis process finishes, heat to 90 ℃ and kept 10 minutes, the deactivation trypsin, (the ultrafilter membrane molecular weight is 10 to protein hydrolyzate through ultrafiltration apparatus in the reaction pot, 000D) separating and filtering, merging ultrafiltration supernatant liquid, is 2 with molecular weight, and the ultrafilter membrane of 000D is concentrated into 20% of initial capacity, be spray dried to dry powder, include the polypeptide and the compositions of abundant anti aging effect.
Embodiment 3
Anti aging effect polypeptide and compositions increase the synthetic proof of collagen in the fibroblast
Cultivate growth of Rama27 rat cell and fusion, use M.J.Warburton, S.A.Ferns and P.S.Rudland, Experimental Cell Research, 137, the method of 378-380 (1980) is measured the speed of their synthetic collagen, add the anti aging effect polypeptide 1mg/ml that embodiment 1 extracts from milk, can make the secretion of collagen improve 12 times, be increased to 663CPM from 54CPM, make the ratio of contained collagen in excretory synthetic collagen and the cell improve nearly twice simultaneously, be increased to 633: 321 (2: 1) from 54: 53 (1: 1).
Embodiment 4
Anti aging effect polypeptide and compositions are to the influence of keratinocyte growth
People's keratinocyte (HatKat) is grown in keratinocyte growth medium (TCS Cellworks Ltd) to be merged until 20%, the hyclone of adding 0.5% is organized in contrast in same culture medium, with adding the polypeptide of embodiment 1 preparation that 0.5% hyclone adds 1mg/ml simultaneously as test group, test group Keratiocyte growth number is that matched group is more than three times as a result.
Claims (10)
1. the present invention relates to the polypeptide and the compositions of a kind of prevention and delaying decrepitude of skin, relate in particular to a kind of polypeptide and compositions of from milk and related raw material, preparing, be used for preventing method with delaying decrepitude of skin, the technical scheme that technical solution problem of the present invention is adopted, it is characterized in that, adopt supermicro filtration membrane or cation exchange resin mode from cattle colostrums, extract in milk or the milk surum, separating natural polypeptide and compositions, adopt protease from milk protein, to prepare polypeptide and compositions by the Proteolytic enzyme mode, natural polypeptides or protolysate independent or the use in conjunction separation and Extraction prepare polypeptide, be applied in the various beauty and make-up products prevention and delaying decrepitude of skin.
2. in accordance with the method for claim 1, technical characterictic of the present invention is, can adopt the ultrafiltration mode from cattle colostrums, extract natural polypeptides and compositions in milk or the milk surum to anti aging effect, its method is, use the defat cattle colostrums, skim milk or milk surum, adopting molecular weight is 8,000～15, the supermicro filtration membrane of 000D, preferably molecular weight is 10, and the supermicro filtration membrane of 000D is used common ultrafiltration apparatus, preferably staggered tangent plane fluid ultrafiltration apparatus, separating and filtering, collect filtering supernatant, the reuse molecular weight is 1, and the 000D ultrafilter membrane is concentrated into 20%～30% of start volume, it is standby to adopt vacuum drying or cryodesiccated mode to be dried to powder, includes abundant polypeptide and compositions to anti aging effect.
3. in accordance with the method for claim 1, technical characterictic of the present invention is, the mode that also can adopt free polypeptide of cationic exchange resin adsorption and compositions is from cattle colostrums, preparation is to the natural polypeptides and the compositions of anti aging effect in milk and the milk surum, its method is, use the defat cattle colostrums, skim milk, milk surum is separated macromolecular substances through the micro-filtrate membrane filtration of 0.1～10.mu.m, can adopt the cation exchange resin of any kind, but preferably select for use average particulate diameter to surpass 100.mu.m, and have a cation exchange resin that enough mechanical strengths are resisted the high flow rate pressure type, should adopt PH is that 5.5～7.5 phosphate buffer comes the pre-equilibration chromatographic column, operating temperature is preferably lower than 10 ℃, to reduce microbial reproduction, crossing the post surface area should be above 500cm/ hour, liquid cross the post rate should 100～600 capacity/hour, after crossing post, with PH is 5.5～7.5, concentration is the following sodium chloride flushing of 0.2mol, it is 5.5～7.5 that eluent should adopt PH, concentration is the sodium chloride solution eluting of 0.25～0.5mol, the eluent molecular weight is 1, after the ultrafilter membrane of 000D is concentrated into initial volume 20%～30%, vacuum drying or lyophilization become powder, include abundant polypeptide and compositions to anti aging effect.
4. in accordance with the method for claim 1, technical characterictic of the present invention is, preparing the present invention by the Proteolytic enzyme mode is milk protein to the raw material of anti aging effect polypeptide and compositions, comprise casein, beta lactoglobulin, albumen in the milk such as alpha-lactalbumin, can adopt milk, cattle colostrums waits the liquid milk raw material clearly, also can adopt casoid flour, albumen dry powder such as the concentrated powder of serum egg albumen powder or serum albumen are as raw material, if select for use albumen dry powder as raw material, casein can be selected acid casein or fat-free milk solids (NFDM) for use, lactalbumin can be selected the milk surum that obtains in the preparation of cheese process for use, especially the dry powder of sweet whey preparation or the protein concentrate that obtains through ultrafiltration, lactalbumin can also be selected for use through ion exchange or electrodialysis and the lactalbumin of demineralization also can be selected for example cheese acid sodium of cheese hydrochlorate for use, dried potassium caseinate or industrial casein are as raw material.
5. in accordance with the method for claim 1, technical characterictic of the present invention is, if adopt milk or cattle colostrums as raw material, need be with the centrifugation defat, adopting ultrafiltration apparatus simultaneously is 30,000～40 with molecular weight, small-molecule substances such as the ultrafilter membrane of 000D demineralizes, lactose, and condensing protein to 10～60 gram albumen/liter, 40～60 gram albumen/rise concentration preferably; If with albumen dry powder or protein liquid concentrated solution as raw material, albumen can dilute or restore into about 10～60 gram albumen/liter, preferably 40～60 gram albumen/liter solution, wherein casein and whey protein proportions are 9: 2～2: 8, preferably 8: 2～6: 4; If the mixture that adopts trypsin and chymase is as protease, tryptic activity is 800～1800USP unit/milligram in this mixture, preferably be equal to or greater than 1800USP unit/milligram, the activity of chymase is 150～350USP unit/milligram, preferably be equal to or greater than 350USP unit/milligram, trypsin is 1～18 with the ratio of chymase, preferably 4～6, the percentage by weight of enzymatic mixture and hydrolyzed protein total amount is 0.2～1.4%, 0.6～0.8% (percentage by weight) preferably, proteoclastic program and method are, protein solution is regulated PH to 6.5～6.8, be heated to 75 ℃～85 ℃ and carried out preheating in about 10～30 minutes, make albumin (BSA) and immunoglobulin (Ig) degeneration, be cooled to the mixture that slowly adds trypsin and chymase after 30 ℃～50 ℃, pass through high pressure, the abundant homogenizing of short time, casein and lactalbumin are well-dispersed in the solution, reach fully and contact with chymase with trypsin, thereby make enzyme digestion reaction carry out more completely, the homogenizing operating pressure is 8～15Mpa, 30 ℃～50 ℃ of hydrolysis temperatures, enzymolysis time continues 4～8 hours, pH value should maintain in 6.5～8 scopes during enzymolysis, when enzymolysis process finishes, should carry out the step of enzyme deactivation by the inactivator activity, can make enzyme deactivation by heat treatment, heating-up temperature to 85 ℃～95 ℃ was kept 10～15 minutes, make enzyme deactivation, collect protein hydrolyzate, with molecular weight is 10, the ultrafilter membrane of 000D carries out isolated by filtration, to remove not the fully macro-molecular protein of hydrolysis, be 1 with molecular weight at last, after the supermicro filtration membrane of 000D concentrates, spraying drying powder-forming includes abundant anti aging effect polypeptide.
6. in accordance with the method for claim 1, technical characterictic of the present invention is, if with the lactalbumin is raw material, comprise lactalbumin powder, whey protein concentrate powder etc., should adding distil water be deployed into 2%～8% protein solution, 4%～6% protein solution (40 grams～60 gram protein/rise solution) preferably can be heated in allotment protein solution process to 40 ℃～50 ℃ and be helped the protein dissolving, and the protein solution of allotment is used for the Proteolytic enzyme process; If with the cheese hydrochlorate, for example cheese acid sodium, dried potassium caseinate are raw material, should adding distil water be deployed into 3% cheese acid salt solution, add 20ml Chymosin (rennet) in per 100 liters of cheese acid salt solutions, under 30 ℃～45 ℃ temperature, kept 40～60 minutes, add sour agent and transfer PH to 4.6～4.8, be heated to 30 ℃～50 ℃ and kept 20～40 minutes, centrifugation or filter type are collected the casein that solidifies, and prepare further to carry out the Proteolytic enzyme program.
7. in accordance with the method for claim 1, technical characterictic of the present invention is, collect the lactoalbumin soln or the casein solution of having allocated, inventory is dissolved in 50 ℃～85 ℃ the distilled water, adds alkali liquor and transfers PH to 7～9, and homogenizing operating pressure 8～15Mpa high pressure homogenize becomes the pasty state base material, press enzyme concentration 0.08%～1.5%, preferably 0.4%～0.6% drop into trypsin, transfer pH value to 3～6, under 37 ℃～54 ℃ temperature are bathed, kept 2～8 hours, caseinhydrolysate, after enzymolysis process is finished, heat rapidly to 10 minutes deactivation trypsin of 85 ℃ of insulations, protein hydrolyzate is 5 by molecular weight, 000～15, the ultrafilter membrane of 000D separates not the fully high molecular weight protein of hydrolysis, collects ultrafiltration supernatant liquid, with molecular weight is 1,000～5,000D, preferably 2,000～3, after the ultrafilter membrane of 000D concentrated, spray drying or vacuum drying became powder, include the polypeptide and the compositions of abundant anti aging effect.
8. in accordance with the method for claim 1, technical characterictic of the present invention is, in order to make the Proteolytic enzyme process abundant, and guarantee that most of protein chains rupture, a kind of mode of improvement can be, after Proteolytic enzyme carries out one hour, continue with staggered tangent plane fluid ultrafiltration apparatus, adopting molecular weight is 8,000～12, the ultrafilter membrane of 000D continues isolated by filtration, molecular weight continues to be collected less than the micromolecule polypeptide in ultrafilter membrane aperture, and molecular weight greater than the ultrafilter membrane aperture not fully the protein molecule of hydrolysis sent back to again and continued hydrolysis in the proteolysis reaction pot, this method not only makes the micromolecule polypeptide that has been hydrolyzed continue to be collected, can also promote the cross reaction contact between albumen and the enzyme, make Proteolytic enzyme more thorough.
9. in accordance with the method for claim 1, technical characterictic of the present invention is, the polypeptide of the anti aging effect of the inventive method preparation and compositions have the effect to anti aging effect, comprise that prevention and delaying skin are loose, skin corrugates, increase skin elasticity, promote promoting epidermization, its effect to anti aging effect passes through to stimulate fibroblastic growth, promotes the synthetic and secretion of collagen, stimulate the growth of keratinocyte, have and the similar effect of fibroblast growth factor (EGF), owing to do not contain protein, so they can sensitization, and product belongs to all-natural product, and life-time service side effect can not occur.
10. in accordance with the method for claim 1, technical characterictic of the present invention is, the present invention is from cattle colostrums, natural polypeptides that extracts in milk and the milk surum and compositions and the polypeptide and the compositions that prepare by Proteolytic enzyme, can use separately or use in conjunction, use in conjunction may be a better choice, the anti aging effect polypeptide of the inventive method preparation and compositions can be used as additive add in all kinds of cosmetics and cosmetics in, include but not limited to protective skin cream, surfactant, cold cream, day cream, late frost, cleansing milk, skin protection oil, cold creams etc. help user prevention and delaying decrepitude of skin.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100161086A CN1557281A (en) | 2004-02-04 | 2004-02-04 | Skin apolexis preventing delaying polypeptide and compositions |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2004100161086A CN1557281A (en) | 2004-02-04 | 2004-02-04 | Skin apolexis preventing delaying polypeptide and compositions |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103705395A (en) * | 2014-01-03 | 2014-04-09 | 广州瑾洋化妆品有限公司 | Whey protein composition and application thereof to face beautifying |
| CN104257597A (en) * | 2014-09-17 | 2015-01-07 | 郑州蓬泽生物科技开发研究院 | Dendrobe beauty fluid and preparation method thereof |
| CN107510619A (en) * | 2017-09-06 | 2017-12-26 | 深圳肽之妃科技有限公司 | A kind of complex polypeptide facial mask and preparation method thereof |
| CN107519037A (en) * | 2017-09-06 | 2017-12-29 | 深圳肽之妃科技有限公司 | A kind of liquid facial mask containing polypeptide and preparation method thereof and application method |
-
2004
- 2004-02-04 CN CNA2004100161086A patent/CN1557281A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103705395A (en) * | 2014-01-03 | 2014-04-09 | 广州瑾洋化妆品有限公司 | Whey protein composition and application thereof to face beautifying |
| CN103705395B (en) * | 2014-01-03 | 2015-11-18 | 广州瑾洋化妆品有限公司 | Lactalbumin composition and the purposes in beauty treatment thereof |
| CN104257597A (en) * | 2014-09-17 | 2015-01-07 | 郑州蓬泽生物科技开发研究院 | Dendrobe beauty fluid and preparation method thereof |
| CN107510619A (en) * | 2017-09-06 | 2017-12-26 | 深圳肽之妃科技有限公司 | A kind of complex polypeptide facial mask and preparation method thereof |
| CN107519037A (en) * | 2017-09-06 | 2017-12-29 | 深圳肽之妃科技有限公司 | A kind of liquid facial mask containing polypeptide and preparation method thereof and application method |
| CN107510619B (en) * | 2017-09-06 | 2020-11-24 | 深圳肽之妃科技有限公司 | Composite polypeptide mask and preparation method thereof |
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