CN1557278A - Skin, hair growth nutrient promotor - Google Patents
Skin, hair growth nutrient promotor Download PDFInfo
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- CN1557278A CN1557278A CNA2004100161090A CN200410016109A CN1557278A CN 1557278 A CN1557278 A CN 1557278A CN A2004100161090 A CNA2004100161090 A CN A2004100161090A CN 200410016109 A CN200410016109 A CN 200410016109A CN 1557278 A CN1557278 A CN 1557278A
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Abstract
The present invention relates to one kind of nutrient promoting skin and hair growth, and is especially one kind of lactoprotein polypeptide prepared via hydrolyzing lactoprotein and the method of using it in promoting body's skin and hair growth. The present invention features that various lactoprotein as material is decomposed by composite proteinase into small molecular weight polypeptide and free amino acid and polypeptide with molecular weight smaller than 1000 D is separated chromatographically. The small molecular weight polypeptide is applied in various cosmetics to promote body's skin and hair growth.
Description
Affiliated technical field
The present invention relates to a kind of nutrient that promotes skin, hair growth, relate in particular to a kind of milk protein peptides that from lactoprotein, prepares by the Proteolytic enzyme method, and be applied to promote the method for human body skin, hair growth.
The invention technical background
For many years, composition is widely used in all kinds of skin type products and the cosmetics in milk and the milk, has announced the dissolving of using milk protein polypeptide and aminoacid increase zinc in the disclosed technology of Japan Patent [No.57 (1982)-209210], reduces the dandruff.The technology introduction that Japan Patent (No.58-500664) is announced use milk protein peptides and prevent and treat the application of dental caries.In the technology that Japan Patent [No.60 (1987)-185100] is announced, having introduced the employing molecular weight and be 500~5,000 milk protein peptides increases the protolysate dissolubility, is applied in the cosmetics.
By further studies confirm that, molecular weight is less than 1 in the milk protein hydrolysate, and the polypeptide of 000D has chafe, hair growth, promotes the effect of absorption of nutrient ingredients, has the good water absorption in surface, the feature of film property.And the composite hydrolysis protein process by the multiple protein enzyme can decompose in the lactoprotein that polypeptide molecular weight is less than 1, and 000D, free amino acid surpass more than 90% of aminoacid problem, thereby make this protolysate have the effect that promotes skin and hair growth.
Technical problem to be solved by this invention is, by the compound protease lactoalbumin hydrolysate, the preparation protein hydrolyzate adopts chromatography method, and the isolated molecule amount is less than 1, and the polypeptide of 000D is applied in the cosmetics, promotes skin and hair growth.
Summary of the invention
The present invention solves the problems of the technologies described above the technical scheme that is adopted, it is characterized in that, with all kinds of lactoprotein is raw material, multiple protein decomposition by the compound protein hydrolytic enzyme, lactoalbumin hydrolysate becomes micromolecule polypeptide and free amino acid, and application layer analysis method isolated molecule amount is less than 1, the polypeptide of 000D, be applied in multiple beauty treatment or the cosmetics, promote the growth of human body skin and hair.
Technical characterictic of the present invention is, milk protein raw material can be any type of milk protein, comprise milk, milk powder, casein or its separator (alpha-casein, beta-casein, Gamma-casein, Kappa-casein), lactalbumin and separator (α-lactoglobulin thereof, beta lactoglobulin), lactalbumin powder, concentrated whey protein powder, high-purity lactalbumin powder etc., also can adopt the lactoprotein of above-mentioned mixed form, when the blending milk protein solution, concentration is in 5%~20% scope.
Technical characterictic of the present invention is, can adopt various types of proteases to carry out the lactoprotein hydrolysis, comprise, trypsin, chymase, subtilisin, papain, thermal decomposition protease, elastoser, proline-specific protease, result from streptococcic protease, result from the protease of lactobacillus or lactobacillus extract etc., the plural protease of use in conjunction simultaneously preferably, for example, the common use of pepsin and pancreatin, the use amount of protease is every gram protein 3,000~5,000 unit, temperature is 40~55 ℃ during enzymolysis, pH value is according to using protease different and different, but should select the pH value of the most suitable use protease, enzymolysis time 2~8 hours, Proteolytic enzyme finishes 90~95 ℃ of post-heating, continue 5~15 minutes, inactivated proteases.
Technical characterictic of the present invention is, the protein hydrolyzate of finishing enzymolysis process is further separated, adopt the method for chromatography, example gel chromatography or hydrophilic chromatography, the absorbing molecules amount is less than 1, the polypeptide of 000D, the chromatographic column inner gel has the feature that absorbs aromatic amino acid and hydrophilic side chain-hydroxyl is arranged, for example, cross-linking dextran G-10, G-15 and G-25 (Sephadex G-10, G-15 and G-25 by pharmacia), Sepharose S-100 (Sephacryl S-100 byPharmasia) etc., then, alcoholic solution eluting with 1%~15%, to increase the aromatic amino acid characterization of adsorption, the separator absworption peak is at 280nm, eluent becomes powder through vacuum drying or lyophilization, includes abundant promotion skin and hair growth nutrient.
Technical characterictic of the present invention is, the inventive method is by in the zymolytic milk protein peptides of compound protein, polypeptide molecular weight is less than 1,000D, wherein free amino acid accounts for more than 90% of total amino acid content, have significant promotion human body skin and hair growth effect, and removed the sensitization of milk protein.
Technical characterictic of the present invention is, the promotion skin of the inventive method preparation, the nutrient of hair growth can be used as additive, add in all kinds of cosmetics and cosmetics in, this nutrient can be dissolved in the water very easily, preparation concentration is 20% aqueous solution, can be added in cosmetics, skin care item and the cosmetics such as protective skin cream, cold cream, day cream, late frost, bath shampoo, shampoo, hair conditioner, cleansing milk, cleansing cream after dilution.
The invention has the beneficial effects as follows, without any side effects when promoting skin and hair growth by adopting the natural nutrition agent, and also raw material sources are abundant, and the course of processing is easy, is fit to apply.
The specific embodiment
The invention will be further described below in conjunction with embodiment:
Embodiment 1
The preparation lactoprotein hydrolysate
Get 400 kilograms of lactalbumin powders, add DDW and be warming up to 45 ℃, continue to stir and dissolve fully until lactalbumin powder, add 1N HCL dilution and regulate PH to 4.6, heat to keep and carried out pasteurization in 60 seconds to 90 ℃, use staggered tangent plane fluid ultrafiltration apparatus, adopting molecular weight is 60, small-molecule substances such as the ultrafilter membrane separate lactose of 000D, collect ultrafiltration concentrate, transfer PH to 2.5 with 1N HCL, add 38.8 liters of cattle pepsin extract (BOVIPEP, bovine pepsin extract.Pepsin Centent of 1.7 gram/L, from Lab.Presure-Granday), hydrolytic process continues 1 hour, add 150 liters of mixed solutions that contain 12 kilograms of NaOH and 18.65 kilograms of KOH, 43 liters of 21% ammonium salt solutions, transfer PH to 8.0 ± 0.1, add the blended protease of 647 grams, contain highly purified trypsin/chymase and (adopt PEM 2500S, trypsin/chymotrypsin protein enzymatic mixture, tryptic activity is greater than 1800USP unit/mg, chymotrypsin activity is greater than 350USP unit/mg, from NOVB INDUSTRIE ENZYMES S.A.Paris.), the II type elastoser (enzymatic activity is greater than 30I.U./mg) that adds 277 grams simultaneously, holding temperature is at 45 ℃ ± 2 ℃, continue 3 hours, the Proteolytic enzyme process finishes, and is warming up to 98 ℃ and continues 60 seconds with the deactivation pancreatin, proteolytic enzyme is pumped into ultrafiltration apparatus, adopting molecular weight is 10, and the SFEC ultrafilter membrane of 000D, ultrafilter membrane surface area are 170m.sup.2, input is pressed and is 2bar., temperature is 50 ℃, collects ultrafiltration supernatant liquid, is further purified separation.
Embodiment 2
Milk protein peptides separates with chromatographic column
Get the protein hydrolyzate of embodiment 1 preparation, (Sephadex G-10 10.times.12cm) crosses chromatographic column, divides flow velocity to spend the ionized water eluting with 10ml/ with cross-linking dextran G-10, eluent been separated in the 280nm absworption peak, and the eluent lyophilization of collection becomes powder.
Claims (6)
1. the present invention relates to a kind of nutrient that promotes skin, hair growth, relate in particular to a kind of milk protein peptides that from lactoprotein, prepares by the Proteolytic enzyme method, and be applied to promote the method for human body skin, hair growth, it is characterized in that, with all kinds of lactoprotein is raw material, multiple protein decomposition by the compound protein hydrolytic enzyme, lactoalbumin hydrolysate becomes micromolecule polypeptide and free amino acid, application layer analysis method isolated molecule amount is less than 1, the polypeptide of 000D, be applied in multiple beauty treatment or the cosmetics, promote the growth of human body skin and hair.
2. in accordance with the method for claim 1, technical characterictic of the present invention is, milk protein raw material can be any type of milk protein, comprise milk, milk powder, casein or its separator (alpha-casein, beta-casein, Gamma-casein, Kappa-casein), lactalbumin and separator (α-lactoglobulin thereof, beta lactoglobulin), lactalbumin powder, concentrated whey protein powder, high-purity lactalbumin powder etc., also can adopt the lactoprotein of above-mentioned mixed form, when the blending milk protein solution, concentration is in 5%~20% scope.
3. in accordance with the method for claim 1, technical characterictic of the present invention is, can adopt various types of proteases to carry out the lactoprotein hydrolysis, comprise, trypsin, chymase, subtilisin, papain, thermal decomposition protease, elastoser, proline-specific protease, result from streptococcic protease, result from the protease of lactobacillus or lactobacillus extract etc., the plural protease of use in conjunction simultaneously preferably, for example, the common use of pepsin and pancreatin, the use amount of protease is every gram protein 3,000~5,000 unit, temperature is 40~55 ℃ during enzymolysis, and pH value is different according to using the protease difference, but should select the pH value of the most suitable use protease, enzymolysis time 2~8 hours, Proteolytic enzyme finishes 90~95 ℃ of post-heating, continues 5~15 minutes, inactivated proteases.
4. in accordance with the method for claim 1, technical characterictic of the present invention is, the protein hydrolyzate of finishing enzymolysis process is further separated, adopt the method for chromatography, example gel chromatography or hydrophilic chromatography, the absorbing molecules amount is less than 1, the polypeptide of 000D, the chromatographic column inner gel has the feature that absorbs aromatic amino acid and hydrophilic side chain-hydroxyl is arranged, for example, and cross-linking dextran G-10, G-15 and G-25 (Sephadex G-10, G-15 and G-25 by pharmacia), Sepharose S-100 (Sephacryl S-100 by Pharmasia) etc., then, the alcoholic solution eluting with 1%~15%, to increase the aromatic amino acid characterization of adsorption, the separator absworption peak is at 280nm, and eluent becomes powder through vacuum drying or lyophilization, includes abundant promotion skin and hair growth nutrient.
5. in accordance with the method for claim 1, technical characterictic of the present invention is, the inventive method is by in the zymolytic milk protein peptides of compound protein, polypeptide molecular weight is less than 1,000D, wherein free amino acid accounts for more than 90% of total amino acid content, has significant promotion human body skin and hair growth effect, and has removed the sensitization of milk protein.
6. in accordance with the method for claim 1, technical characterictic of the present invention is, the promotion skin of the inventive method preparation, the nutrient of hair growth can be used as additive, add in all kinds of cosmetics and cosmetics in, this nutrient can be dissolved in the water very easily, preparation concentration is 20% aqueous solution, can be added in cosmetics, skin care item and the cosmetics such as protective skin cream, cold cream, day cream, late frost, bath shampoo, shampoo, hair conditioner, cleansing milk, cleansing cream after dilution.
Priority Applications (1)
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CNA2004100161090A CN1557278A (en) | 2004-02-04 | 2004-02-04 | Skin, hair growth nutrient promotor |
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CNA2004100161090A CN1557278A (en) | 2004-02-04 | 2004-02-04 | Skin, hair growth nutrient promotor |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102665751A (en) * | 2009-12-24 | 2012-09-12 | 罗蒂株式会社 | Anti-androgenic agent, sebum secretion blocker, hair growth stimulant, and food or beverage |
CN107952068A (en) * | 2017-12-25 | 2018-04-24 | 彭光简 | A kind of anti-infective ointment of Traditional Chinese medicine probiotic |
CN110680761A (en) * | 2019-10-28 | 2020-01-14 | 佛山市安安美容保健品有限公司 | Liquid shampoo |
-
2004
- 2004-02-04 CN CNA2004100161090A patent/CN1557278A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102665751A (en) * | 2009-12-24 | 2012-09-12 | 罗蒂株式会社 | Anti-androgenic agent, sebum secretion blocker, hair growth stimulant, and food or beverage |
CN102665751B (en) * | 2009-12-24 | 2014-04-30 | 罗蒂株式会社 | Anti-androgenic agent, sebum secretion blocker, hair growth stimulant, and food or beverage |
CN107952068A (en) * | 2017-12-25 | 2018-04-24 | 彭光简 | A kind of anti-infective ointment of Traditional Chinese medicine probiotic |
CN110680761A (en) * | 2019-10-28 | 2020-01-14 | 佛山市安安美容保健品有限公司 | Liquid shampoo |
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