JP2834278B2 - Cosmetic and external preparation for skin - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Definition of Terms] In the present invention, "milk protein" is defined as casein, casein-fractionated _αs -casein, β-casein, γ-casein, κ-casein, whey protein, Whey protein is one or a mixture of α-lactalbumin and β-lactoglobulin fractionated from whey protein, and “hydrolysate” is a peptide obtained by decomposing milk protein with a protease. 90% of the aromatic amino acids contained
(Weight. The same applies hereinafter.) The hydrolyzate is a free amino acid and has no antigenicity as a milk protein and / or a salt thereof. The “hydrolyzate fraction” is separated from the hydrolyzate. A peptide mixture having a molecular weight of 1000 or less, having an aromatic amino acid content of 5% or less based on the total amino acid content thereof, having a growth stimulating activity on human skin cells, and It is a specific fraction having no antigenicity as a milk protein and / or a salt thereof, and "cosmetics" is a product contained in any of the cosmetics, quasi-drugs, and pharmaceuticals referred to in the Pharmaceutical Affairs Law. , Lotion, cream, emulsion, pack, foundation, face wash, soap,
Cosmetic products such as hair cosmetics and hair wash cosmetics, and external preparations for skin.

[Industrial application field] The present invention relates to a cosmetic or the like characterized by containing a fraction of a specific milk protein hydrolyzate as an active ingredient,
More specifically, the present invention relates to cosmetics and the like which do not show the antigenicity of milk protein, impart a proliferation activity to human skin cells, have an effect of recovering from rough skin, smoothing the texture of the skin, and smoothing the skin.

[Background of the Technology and Description of the Prior Art] Conventionally, a technique of blending a peptide or amino acid obtained by hydrolyzing milk protein into a cosmetic or the like is known. For example, there are JP-A-57-209210, JP-A-58-500664, JP-A-59-152317, and JP-A-63-57515. These prior arts are intended to promote the solubilization of zinc pyrithione, suppress dental decay, and be used as a component of oral products or baths. In addition,
62-185100, JP-A-64-11 and JP-A-1-
Japanese Patent No. 269499 discloses a technique of removing potentially insoluble large molecular components by an ultrafiltration method, a pH adjustment method, etc., and blending the same into a cosmetic or the like. The reason that these components are blended into cosmetics and the like is mainly due to the skin beautiful effect by the moisturizing, adsorbing, film-forming or protecting effects of these components.

These components in the prior art merely hydrolyze or simply fractionate the milk protein and are not intended to obtain a fraction having particular physicochemical and / or biological properties. .

Some of the present inventors have previously described protein in milk for the purpose of solubilizing milk protein, the effectiveness of digestion and absorption, prevention and treatment of dietary allergy, treatment of aromatic amino acid metabolism disorders and nutritional supplementation. A patent application was filed for the invention of a hydrolyzate having no antigenicity, a fraction thereof and a production method thereof. (Japanese Patent Application No. 63-291090, hereinafter referred to as prior application). The present inventors have conducted various studies on the fraction obtained by the prior application, and as a result, it was found that the fraction obtained by the method of the prior application showed a moisturizing action and a beautiful skin of a conventionally known milk protein hydrolyzate. In addition to the effect, the present inventors have found that they have a growth activating effect on human skin cells, and thus completed the present invention.

[Object of the Invention and Summary of the Invention] An object of the present invention is to provide a cosmetic or the like in which a hydrolyzate fraction containing a peptide imparting proliferative activity to human skin cells is blended.

Another object of the present invention is to provide a cosmetic or the like containing a hydrolyzate fraction containing a peptide that does not exhibit the antigenicity of milk protein.

The present invention relates to a specific fraction of a peptide mixture obtained by hydrolyzing milk protein, wherein the peptide has a molecular weight of 10
A hydrolyzate fraction having an aromatic amino acid content of 5% or less based on the total amino acid content, having a growth activating effect on human skin cells, and having no antigenicity of milk protein And / or a salt thereof as an active ingredient.

[Specific description of the invention] The milk proteins used in the present invention are all commercially available or easily available, and are usually dissolved in water at a concentration of 5 to 20% or concentrated to the same concentration. Or prepared by:

The hydrolyzate used for preparing the hydrolyzate fraction is prepared as follows.

The enzymes used for the hydrolysis of milk proteins are not particularly limited, and commercially available products such as trypsin, chymotrypsin, subtilisin, elastase, proline-specific protease, proteases produced by microorganisms of the genus Streptococcus, papain, pepsin, and thermolysin are used. it can.
In addition, carboxypeptidase Y, a protease produced by a microorganism of the genus Aspergillus, a protease produced by a microorganism of the genus Streptomyces, a protease produced by a microorganism of the genus Rhizopus, a protease produced by a lactic acid bacterium, a protease produced by a lactic acid bacterium, and the like are also used as exopeptidases. it can. As the lactic acid bacteria extract, for example, a lactic acid bacteria extract having 20,000 activity units per gram can be obtained by the method described in JP-B-48-43878.

The amount of enzyme used for the hydrolysis of milk protein is 1 g of milk protein
Per pancreatin and exopeptidase or pancreatin and other proteases and exopeptidase are mixed or added in a ratio of 3000 to 5000 activity units per unit.

The hydrolysis is carried out at a temperature of 40 to 55 ° C., and the pH is adjusted to an optimum pH within a range that does not denature the milk protein before addition of the enzyme, and is preferably maintained for at least 1 hour.

After the completion of the hydrolysis, the enzyme is deactivated by heating, and after cooling, filtration, desalting, concentration and drying are performed as necessary to obtain a hydrolyzate. This hydrolyzate does not show milk protein antigenicity by the same test method as in Test Example 1.

The desired fraction is separated from the hydrolyzate by gel filtration, hydrophobic chromatography and the like. For example, when fractionation is performed by gel filtration, the exclusion limit molecular weight is 10,000 or less, preferably a gel filtration agent of 1,000 or less, and more preferably, a hydrophobic side chain that does not show adsorptivity to aromatic amino acids, such as a carboxyl group or the like. Gel carriers having a butyl group, a phenyl group or a hydrophobic site [for example, Sephadex G-10, G-15
And G-25 (both manufactured by Pharmacia), Sephacryl S-100 (manufactured by Pharmacia), Toyopearl EW-40 (manufactured by Tosoh Corporation), and the like. Alternatively, elution is performed with a 2 to 15% ethanol solution for the purpose of enhancing the adsorptivity of aromatic amino acids, and fractionation is performed. The fractionation is performed based on the absorption at 280 nm, and when four fractions from the first elution peak (peak) to the fourth elution peak are obtained in the order of elution depending on the type of the gel carrier, or when the first elution peak and the second elution peak are obtained. A first elution peak in which a peak corresponding to an elution peak is one elution peak, a second elution peak corresponding to the third elution peak, and the fourth elution peak.
In some cases, three fractions of the third elution peak corresponding to the elution peak may be obtained. In any case, the third and fourth elution peaks (in the former case), the second and third elution peaks (in the latter case), which later elute, have a high aromatic amino acid content and a high water content relative to water. Due to its low solubility, it is not suitable for use in the cosmetics of the present invention. Therefore, in the present invention, the first elution peak and / or the second elution peak are used. The fractionated fraction is concentrated and dried as necessary to obtain a hydrolyzate fraction.

The hydrolyzate fraction used in the present invention is prepared as described above.

Cosmetics or the like using the hydrolyzate fraction are produced by a conventional method. The hydrolyzate-enriched fraction is easily soluble in water used for production at a concentration of 20% or less, and a predetermined amount is dissolved in, for example, purified water, and mixed, emulsified, and dispersed with other components. If the concentration of the hydrolyzate fraction exceeds 20%,
The solubility gradually decreases as the concentration increases.

As salts of the hydrolyzate fraction, organic and inorganic salts acceptable as pharmaceuticals, quasi-drugs and cosmetics such as sodium and potassium are used.

If necessary, emulsifiers, fragrances, and other components acceptable as pharmaceuticals, quasi-drugs, and cosmetics can also be used at the same time.

 Next, the present invention will be described in detail with reference to test examples.

(Test Example 1) This test was performed to examine the physicochemical properties of the hydrolyzate fraction.

(1) Preparation of hydrolyzate fraction 1) First elution peak (sample A), second elution peak (sample B), third elution peak (sample C) prepared in the same manner as in Reference Example 1. , And the fourth elution peak (Sample D).

2) Using the first elution peak (sample E), the second elution peak (sample F), the third elution peak (sample G), and the fourth elution peak (sample H) prepared in the same manner as in Reference Example 2. Was.

(2) Measurement of aromatic amino acid content The total amino acid content was analyzed with an automatic amino acid analyzer, the aromatic amino acid content relative to the total amino acid content was calculated, and the aromatic amino acid content was tested. In addition, about the hydrolyzate, the free amino acid content was also analyzed.

Table 1 shows the results of amino acid analysis of the samples A to H prepared in the above (1).

As is clear from Table 1, the content of aromatic amino acids in Samples A and B is 5% or less, the content of aromatic amino acids in Samples C and D is remarkably high at 40% or more, and their solubility is 2% or less. It was remarkably low. Similar results were observed in samples EH. The aromatic amino acid content of the cosmetic casein peptide having an average molecular weight of 100 to 3,000 produced by the conventional method (the same method as in Example 1 described in JP-A-1-269499; hereinafter the same) was more than 5%. Was.

(3) Measurement of molecular weight Samples A) and E) prepared in the same manner as in the above (1) were eluted with 0.5 N acetic acid using Sephadex G-25 to measure the molecular weight. As a result, the first elution peaks of the samples A) and E), which were eluted by the gel filtration method, eluted later than the oxytocin (molecular weight: 1000) used as the standard substance.
It was also found that the molecular weight of each of the samples E to H was 1000 or less.

(Test Example 2) This test was performed to examine the antigenicity of the hydrolyzate fraction.

Sample A prepared by the same method as in Test Example 1
And B were measured for antigenicity by the ELISA inhibition test method. Using a 96-well plate (manufactured by Nunc), coating the starting protein, washing, reacting a mixed solution of rabbit antiserum prepared by sensitizing the starting protein with various hydrolyzate fractions, and washing. Thereafter, an alkaline phosphatase-labeled goat anti-rabbit IgG antibody (manufactured by Zymed Laboratories) was reacted. After washing, an enzyme substrate, sodium p-nitrophenylphosphate, was added. After 30 minutes, the reaction was stopped with 5N sodium hydroxide. And the reaction products were measured with a microplate reader (Journal of the Toho Medical Association, Vol. 35, p. 509, 1989). As a result, the concentration of various samples reacted with rabbit antiserum was the highest concentration in the ELISA test.
Be increased up to 10 ⁵ [mu] g / ml, the inhibition rate of both samples is 20% or less, both samples were confirmed to have lost the antigenic casein. Similarly, in samples E) and F, no antigenicity of whey protein was observed.

(Test Example 3) This test was performed to examine the proliferation activating activity of the hydrolyzate fraction on human skin cells.

The samples A and B prepared in Test Example 1 above, a commercially available bovine placenta extract (manufactured by Botoger, West Germany), and the sample I prepared by a conventional method were tested for the growth activating activity of human skin cells by the following method.

Epithelial cells derived from human normal skin were cultured in a modified KGM medium and fibroblasts were cultured in a modified MEM medium containing 2% fetal bovine serum, respectively, by a conventional method. In the medium, samples A, B, bovine placenta extract and sample I were prepared at 1,10 and
100 μg / ml was added, culture was continued for 2 to 3 days, and then thymidine labeled with [ ³ H] was added, culture was continued for another 2 to 3 hours, and then the amount of [ ³ H] incorporated into cells Was measured with a liquid scintillation counter. Each medium without any addition was used as a control.

 The results of this test were as shown in Table 2.

As is clear from Table 2, the samples A and B have remarkable growth stimulating effects on human skin cells, and have the same or twice the stimulating effect as bovine placenta extract, which has been widely known to have a conventionally known growth stimulating effect on human skin cells. Had. In contrast, sample I was as effective as the control. still,
Samples E and F prepared in (1) of Test Example 1 also showed a remarkable growth-activating effect on human skin cells, similarly to Samples A and B.

(Test Example 4) This test was performed to examine the effect of cosmetics and the like depending on the presence or absence of a hydrolyzate fraction.

(1) Preparation of samples Fourteen kinds of samples (samples 1 to 14) containing hydrolyzate fractions were prepared by the same formulation and production method as in Examples 1 to 14, and the hydrolyzate having the following formulation was prepared. Seven samples without fractions (A
-G: control) were prepared by a conventional method. In addition, as is clear from the composition of the Example and the following composition, Samples 1 and 2 correspond to A, and Samples 3 and 4 correspond to B.

1) Sample A A skin cream having the following composition was produced.

Stearic acid 15.0 (%) Cetanol 2.0 Squalane 3.0 Octyldodecyl myristate 5.0 Glycerin 10.0 1,3-butylene glycol 4.0 Polyoxyethylene monostearate (20 mol) Sorbitan 3.0 Purified water 58.0 2) Sample B Make up lotion with the following formulation did.

Propylene glycol 10.0 (%) Oleyl alcohol 0.1 Hydrogenated castor oil polyoxyethylene (60 mol) adduct 1.5 Ethanol 5.0 Purified water 83.4 3) Sample C A beauty serum having the following composition was prepared.

Sodium hyaluronate 0.1 (%) Placenta extract 1.5 Glycerin 1.0 Purified water 97.4 4) Sample D A skin external ointment having the following formulation was produced.

Vaseline 25.0 (%) Paraffin 5.0 Cetostearyl alcohol 2.0 Propylene glycol 10.0 Polyoxyethylene / polyoxypropylene glycol ether 3.0 Purified water 55.0 5) Sample E A hair treatment having the following composition was produced.

Cetanol 1.5 (%) 2-hexyldecanol 1.0 1,3-butylene glycol 3.0 cationized cellulose 0.2 polyoxyethylene stearyl ether 1.0 purified water 93.3 6) Sample F A hair rinse having the following composition was produced.

Dialkyldimethylammonium chloride 2.0 (%) Cetanol 1.0 Polyoxyethylene stearyl ether 1.0 1,3-butylene glycol 3.0 Cationized cellulose 0.2 Purified water 92.8 7) Sample G A hair shampoo having the following formulation was produced.

Triethanolamine lauryl sulfate 15.0 (%) 1,3-butylene glycol 2.0 Ethylene glycol monostearate 1.5 Purified water 81.5 (2) Test method 1) Testing of skin cosmetics, etc. Five expert panels were selected, the inside of the left and right hand arms was thoroughly washed with a commercially available toilet soap, and it was confirmed that there was no residual soap. Samples 1 to 4 and Samples A and B (control ), Samples 5 to 8 and Samples C and D (control) inside the right arm
Each pair was coated with 0.5 g of each about 30 mm × 15 mm and rubbed into the skin. Rub this skin daily
Four times a week, and once a week, the determination was made by the following method.

Observe the rubbing area of each sample with the naked eye to prevent rough skin,
For recovery from rough skin, smoothing skin, adjusting skin texture, applying skin tension, protecting skin, and softening skin, +2 if control sample is significantly better than control sample. + If slightly better than sample
1, 0 if equal to control sample, -1 if slightly worse than control sample,-if significantly worse than control sample
Evaluation was made in five steps of 2, the average value was calculated, and the effect was tested.

2) Testing of hair cosmetics, etc. 20 to 40 with obvious hair damage and pastsuki
We chose 30 expert panels of age group and divided them into 6 groups of 5
Samples 9 and E were tested in the first group, samples 10 and E in the second group, and samples 11 and F in the third group. First group ~
Wash the hair of the panel of the fourth group thoroughly with a commercial shampoo, confirm that no shampoo remains, apply 2 g of each sample to the hair on both sides of the temporal region about 50 mm x 80 mm, and rub by hand I was crowded. In the third and fourth groups for testing the hair rinse, the hair was lightly washed with water immediately after the sample was rubbed. The hair was rubbed once every four days, washed on the fourth day, and evaluated every four days ten times by the following method. In the fifth and sixth groups in which shampoos were tested, the hairs which had been divided into right and left by Samples 13 and G or Samples 14 and G were not washed with a commercially available shampoo. The hair was washed once a day for 15 consecutive days, and was evaluated by the following method immediately before daily hair washing.

Visually observe the rubbing area of each sample to recover damaged hair,
For each item of prevention of cut and split ends, flexibility of hair, smoothness after finishing, disappearance of pastsuki, and gloss of hair, +2 when considerably better than control sample, and slightly better than control sample Is evaluated in five steps: +1, 0 when equivalent to the control sample, -1 when slightly poorer than the control sample, and -2 when significantly worse than the control sample, calculate the average value, and evaluate the effect. Tested.

(3) Test results The results of this test are as shown in Tables 3 and 4.
As is clear from Table 3, in the products for skin, the effects of preventing rough skin, recovering from rough skin and giving skin firmness were observed in the products containing sample A or E, and the rough skin was found in the products containing sample B or F. A recovery effect was observed.

As is clear from Table 4, in the hair products, the effects of recovering damaged hair and imparting smoothness to the finished product containing the samples A and E, and the recovery of damaged hair on the products containing the samples B and F are also shown. The effects were each observed.

As described above, the combination of the fractions showed excellent effects on both skin products and hair products.

(Test Example 5) This test was performed to examine the amount of the hydrolyzate fraction added.

(1) Preparation of Sample A skin cream and a hair treatment were manufactured according to the same formulation as in Examples 1 and 9. However, the amount of the fraction added was adjusted to 0.01 to 50% as shown in Table 5, and the amount of purified water was increased or decreased accordingly. A sample containing no hydrolyzate fraction was prepared in the same manner as Samples A and E of Test Example 4 and used as a control.

(2) Test method The same method as in Test Example 4 was used. However, skin cream was similarly evaluated based on one item of recovery from rough skin, and hair treatment was similarly evaluated based on one item of recovery of damaged hair.

(3) Test results The results of this test were as shown in Table 5. With skin creams and hair treatments, the above effect is remarkably observed with an increase in the amount of the hydrolyzate fraction added, and the effect is maximized in the range of 1 to 20%, and 30 to 50%.
%. The desired addition amount of the fraction is less than 20%, more preferably 0.01-20%, because of the reduced solubility of the hydrolyzate fraction in water and the stickiness of the product.

(Test Example 6) This test was performed to examine the amount of the hydrolyzate fraction added to products other than the products tested in Test Example 5.

(1) Preparation of sample A lotion, a serum, a skin external ointment, a hair rinse, and a hair shampoo were manufactured by the same formulation as in Examples 3 to 8 and 11 to 14. However, the amount of the fraction added was adjusted to 0.01%, 1.0%, 20%, 30% and 50% as shown in Table 5, and the amount of purified water was increased or decreased accordingly. A sample containing no hydrolyzate fraction was prepared in the same manner as samples B, C, D, F and G of Test Example 4 and used as a control.

(2) Test method The same method as in Test Example 5 was used.

(3) Test results The results of this test were as shown in Table 6. As is evident from Table 6, the addition amount of the desired hydrolyzate fraction in these products was 0.01 to 20%.

Next, a production example of a hydrolyzate fraction used in the present invention will be described.

Reference Example 1 200 g of commercially available casein was suspended in water to a concentration of 10%.
The pH was adjusted to 8.0 with a 10% aqueous sodium hydroxide solution.
The casein solution was sterilized by heating at 90 ° C. for 10 minutes, cooled to 45 ° C., added with 10 g of pancreatin F (Amano Pharmaceutical), 2 g of protease N “Amano” (Amano Pharmaceutical), and 4 g of the lactic acid bacterium extract. Hydrolysis for hours. The digest was heated at 90 ° C. for 5 minutes to inactivate the enzyme and filtered to remove the precipitate.
This was freeze-dried to obtain about 165 g of a powder hydrolyzate.
(The hydrolyzate was tested in the same manner as in Test Examples 1 and 2, and as a result, the release rate of the aromatic amino acid was 90.5%, and the antigenicity of casein was not recognized.) Dissolve 16g in water at a concentration of 20%,
The solution was passed through a column (10 × 12 cm) packed with Sephadex G-10, and eluted with ion-exchanged water at a flow rate of 10 ml / min. 280
Based on the absorbance curve at nm, the fraction was separated into four fractions, a first elution peak, a second elution peak, a third elution peak, and a fourth elution peak, and lyophilized. 1.6 g and about 0.3 g were obtained.

Reference Example 2 200 g of a commercially available whey protein powder was dissolved in deionized water at a concentration of 8%. The bacteria were removed by filtration through a filter, the temperature was adjusted to 45 ° C., and 2 g of pancreatin F (Amano Pharmaceutical) was added while maintaining the pH at 7.5 by adding a 5% aqueous sodium hydroxide solution.
The solution was added six times at 30 minute intervals, and after 15 hours, 2.0 g of actinase AS (Kaken Pharmaceutical) was added, followed by further decomposition for 5 hours. The enzyme was inactivated by heating at 90 ° C. for 5 minutes and filtered to remove the precipitate. This was freeze-dried to obtain about 160 g of a powdered hydrolyzate.
(The hydrolyzate was tested in the same manner as in Test Examples 1 and 2, and as a result, the aromatic amino acid release rate was 90.4%, and the antigenicity of whey protein was not recognized.)

Dissolve 100 g of the obtained hydrolyzate in water at a concentration of 10%,
The solution was passed through a column (37 × 15 cm) packed with Sephadex G-10, and eluted at a flow rate of 200 ml / min of ion-exchanged water. 280n
Based on the absorbance curve at m, fractionation into four fractions, a first elution peak, a second elution peak, a third elution peak, and a fourth elution peak, lyophilization, and about 54 g, about 33 g, about 8 g, And about
1.5 g were obtained.

 Next, the present invention will be described in more detail with reference to examples.

In addition, since each Example was manufactured by the usual method, only the combination was shown.

Example 1 A skin cream having the following composition was produced.

Stearic acid 15.0 (%) Cetanol 2.0 Squalane 3.0 Octyldodecyl myristate 5.0 Glycerin 10.0 1,3-butylene glycol 4.0 Polyoxyethylene monostearate (20 mol) sorbitan 3.0 First elution peak of Reference example 1 5.0 Purified water 53.0 Example 2 A skin cream having the following formulation was produced.

Stearic acid 15.0 (%) Cetanol 2.0 Squalane 3.0 Octyldodecyl myristate 5.0 Glycerin 10.0 1,3-butylene glycol 4.0 Polyoxyethylene monostearate (20 mol) Sorbitan 3.0 Second elution peak of Reference example 1 5.0 Purified water 53.0 Example 3 A lotion having the following composition was produced.

Propylene glycol 10.0 (%) Oleyl alcohol 0.1 Hardened castor oil Polyoxyethylene (60 mol) adduct 1.5 Ethanol 5.0 First elution peak of Reference example 3.5 Purified water 79.9 Example 4 A lotion having the following composition was prepared.

Propylene glycol 10.0 (%) Oleyl alcohol 0.1 Hydrogenated castor oil Polyoxyethylene (60 mol) adduct 1.5 Ethanol 5.0 Second elution peak of Reference example 3.5 Purified water 79.9 Example 5 A beauty serum having the following composition was produced.

Sodium hyaluronate 0.1 (%) Placenta extract 1.5 Glycerin 1.0 First elution peak of Reference example 2 4.0 Purified water 93.4 Example 6 A cosmetic solution having the following formulation was produced.

Sodium hyaluronate 0.1 (%) Placenta extract 1.5 Glycerin 1.0 Second elution peak of Reference example 2 4.0 Purified water 93.4 Example 7 An external ointment for skin having the following formulation was produced.

Vaseline 25.0 (%) Paraffin 5.0 Cetostearyl alcohol 2.0 Propylene glycol 10.0 Polyoxyethylene / polyoxypropylene glycol ether 3.0 First elution peak of Reference example 5.0 Purified water 50.0 Example 8 An external ointment having the following formulation was produced.

Vaseline 25.0 (%) Paraffin 5.0 Cetostearyl alcohol 2.0 Propylene glycol 10.0 Polyoxyethylene / polyoxypropylene glycol ether 3.0 Second elution peak of Reference example 5.0 Purified water 50.0 Example 9 A hair treatment having the following composition was produced.

Cetanol 1.5 (%) 2-hexyldecanol 1.0 1,3-butylene glycol 3.0 cationized cellulose 0.2 polyoxyethylene stearyl ether 1.0 First elution peak of Reference Example 2 4.0 Purified water 89.3 Example 10 A hair treatment having the following composition was produced. .

Cetanol 1.5 (%) 2-hexyldecanol 1.0 1,3-butylene glycol 3.0 cationized cellulose 0.2 polyoxyethylene stearyl ether 1.0 Second elution peak of Reference Example 2 4.0 Purified water 89.3 Example 11 A hair rinse having the following composition was produced.

Dialkyldimethylammonium chloride 2.0 (%) Cetanol 1.0 Polyoxyethylene stearyl ether 1.0 1,3-butylene glycol 3.0 Cationized cellulose 0.2 First elution peak of Reference example 4.5 Purified water 88.3 Example 12 A hair rinse having the following composition was produced. .

Dialkyldimethylammonium chloride 2.0 (%) Cetanol 1.0 Polyoxyethylene stearyl ether 1.0 1,3-butylene glycol 3.0 Cationized cellulose 0.2 Second elution peak of Reference example 4.5 Purified water 88.3 Example 13 Preparation of hair shampoo having the following formulation did.

Triethanolamine lauryl sulfate 15.0 (%) 1,3-butylene glycol 2.0 Ethylene glycol monostearate 1.5 First elution peak of Reference Example 2 5.0 Purified water 76.5 Example 14 A hair shampoo having the following composition was produced.

Triethanolamine lauryl sulfate 15.0 (%) 1,3-butylene glycol 2.0 Ethylene glycol monostearate 1.5 Second elution peak of Reference Example 5.0 Purified water 76.5 [Effect of the Invention] The effects of the present invention are as follows. It is.

(1) A skin product having an excellent effect of recovering from skin roughness and preventing skin roughness is obtained.

(2) A hair product having an excellent effect of recovering damaged hair can be obtained.

Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 38/00 A61K 37/18 38/17 37/16 (72) Inventor Seiji Kawaura 1-7-11 Doshomachi, Chuo-ku, Osaka-shi, Osaka (72) Inventor Yasuo Fukuwatari 3-1-4-103 Nijigaoka, Aso-ku, Kawasaki City, Kanagawa Prefecture (72) Inventor Masanobu Nojiri 2-14-19 Tsukushino, Machida-shi, Tokyo (56) References JP JP-A-1-269499 (JP, A) JP-A-4-26604 (JP, A) JP-A-63-30404 (JP, A) JP-B-7-73507 (JP, B2) (58) Fields investigated (Int) .Cl. ⁶ , DB name) A61K 7/00-7/50 A61K 37/02

Claims (1)

(57) [Claims] 1. A cosmetic comprising a fraction of an enzymatic hydrolyzate of milk protein having the following characteristics (a) to (d) and / or a salt thereof: (a) Sephadex G A peptide mixture having a molecular weight of 1,000 or less as eluted from 0.5 N acetic acid using -25, and (b) an aromatic amino acid content of 5 to the total amino acid content.
% (Weight) or less; (c) having a growth activating effect on human skin cells; and (d) having no antigenicity of milk protein.