CN1541538A - Chinese herbal medicine air disinfectant - Google Patents
Chinese herbal medicine air disinfectant Download PDFInfo
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- CN1541538A CN1541538A CNA031167004A CN03116700A CN1541538A CN 1541538 A CN1541538 A CN 1541538A CN A031167004 A CNA031167004 A CN A031167004A CN 03116700 A CN03116700 A CN 03116700A CN 1541538 A CN1541538 A CN 1541538A
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- herbal medicine
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Abstract
The air sterilizer of Chinese herbal medicine contains honeysuckle extracting liquid 0.5-2 wt%, moxa leaf oil 0.2-3 wt%, propylene glycol 0-3 wt% and lactic acid 0-3 wt% except ethanol. It has high safety and may be sprayed in manned condition, and it has respiratory track infection preventing effect higher that of ultraviolet radiation plus white vinegar fumigation.
Description
Technical field
The present invention relates to disinfectant, be specifically related to air sanitizer, more specifically relate to new Chinese herbal medicine air sanitizer.
Background of invention
Contact at most with the mankind, the easiest harm humans health be airborne pathogenic microorganism, especially in using family, office building and the public place of air-conditioning, contamination by micro is the main paathogenic factor that causes respiratory tract infection.Therefore, the sterilization of air has caused people's attention widely.But what use at present both at home and abroad all is chemosterilant, and human body is had certain excitant or toxicity, and object is corrosive more, is not suitable for being used in family and public place under people's the situation having.Therefore, developing safely and effectively, air sanitizer has become pressing for of current society.
Summary of the invention
An object of the present invention is to provide safe, can the Chinese herbal medicine air sanitizer that use under people's the situation arranged.
Another object of the present invention provides the using method of this Chinese herbal medicine air sanitizer.
Chinese herbal medicine air sanitizer provided by the invention comprises:
Flos Lonicerae extractive solution 0.5-2%
Blumea oil 0.2-3%
A preferred embodiment of the present invention is also to comprise in the Chinese herbal medicine air sanitizer:
Propane diols 0-3%
The ethanol surplus
Another preferred embodiment of the present invention is also to comprise in the Chinese herbal medicine air sanitizer:
Lactic acid 0-3%
Medium-height grass thing honeysuckle has the effect of sterilization and resisiting influenza virus, and its main active ingredient is chlorogenic acid (chemical name: 3-(3,4-dihydroxy cinnamoyl) quininic acid; C16H18O9), extract, make that chlorogenic acid content reaches higher level in the Flos Lonicerae extractive solution by scientific methods, pathogenic microbe killing effectively, thus reach the purpose of air sterillization.In addition, in traditional medium-height grass thing, the volatile oil of tarragon also has kills pathogenic bacteria and viral effect in the air, simultaneously, also has the effect that makes air delicate fragrance fragrance.This product is main disinfection composition with the Flos Lonicerae extractive solution that contains the high concentration chlorogenic acid; Be aided with volatile argyi leaf oil the Disinfection Effect of product is further strengthened, and have the pleasant smell of nature.
Add 1 in the Chinese herbal medicine air sanitizer of the present invention, 2-two propyl alcohol are the particulate in the absorbed air effectively, and the active ingredient in the product is fully contacted with airborne pathogenic microorganism, reaches Disinfection Effect, and reduces the excitant of product; Add lactic acid, can play stabilization; Ethanol helps the even dispersion of active ingredient as solvent, and itself also has disinfective action.
Chinese herbal medicine air sanitizer of the present invention can spray in people or nobody environment are arranged.Preferably adopt sprayer to spray.Sprinkling can be adopted stoste, and consumption is 0.5-2ml/m
3Spray after also can adopting stoste dilution 2-3 doubly.
Embodiment
The present invention is further elaborated with the test example below in conjunction with embodiment, but these embodiment and test example never are any limitation of the invention.
Embodiment 1
1. use the alcohol 95-105kg that contains 5% acetate with honeysuckle 80kg soaked overnight, filter, concentrate, obtain Flos Lonicerae extractive solution 50kg.
2. by 5% sodium chloride: tarragon=1: 1, after the immersion, steam distillation obtains Blumea oil.
By following prescription with each composition mix, filtration, can.
Flos Lonicerae extractive solution 0.5-2%
Blumea oil 0.2-3%
Propane diols 0-3%
Lactic acid 0-3%
The ethanol surplus
The inhibitory action of test example 1 pair of staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans
According to higher medical institute teaching material " clinical microbiology and check " (Li Yinglin chief editor, nineteen ninety-five version), with four kinds of Chinese medicine extracts (D1-D4) with the medium of various dose and thawing in plate fully behind the mixing, cooling promptly becomes content 1: 10,1: 25,1: 50.Quantitatively inoculate staphylococcus aureus, Pseudomonas aeruginosa and three kinds of bacterium liquid of Candida albicans then, hatched 18,36 hours, observe the bacterial growth situation.
The result shows, four kinds of Chinese medicine extracts (D1-D4) all have in various degree inhibitory action to Candida albicans, staphylococcus aureus and Pseudomonas aeruginosa.Its action effect is: D1＞D4＞D3＞D2.
Test example 2 air sterillizations test
Test material:
1. experiment cabinet: 1M
3The aerosol chamber, test temperature is 26-30 ℃, relative moisture 70%-85%.
2. aerosol generator: full glass concentric circles sprayer, atomisation pressure is 2kg/cm
2
3. aerosol sampler: full glass precursor solution impact-actuated sampler, sampling flow is 10 liters/minute.
4. test strain: staphylococcus aureus ATCC 6538 strains, during test in the cabinet aerosol concentration 1 * 10
8Cfu/m
3About.Candida albicans ATCC 10231 strains, the interior aerosol concentration of cabinet is 1 * 10 during test
7Cfu/m
3About.
5. the Chinese herbal medicine air sanitizer is used its stoste during test.
Test method:
1. neutralizer test: adopt dilution method.With 3 * 10
4Cfu/ml test organisms bacteria suspension adds in the aerosol sampler respectively.At 1m
3Aerosol cabinet spray medicine number minute post-sampling 1 minute was a spray medicine group, at 1m
3Several minutes post-samplings of water spray 1 minute be control group in the aerosol cabinet, carried out count plate with conventional method, relatively sprayed the medicine group with control group bacterium number has indifference, and the interior disinfectant of observation aerosol cabinet has or not influence to the test organisms in the sampler.
2. bactericidal assay: sterilization group and control group are established in test.During test to 1m
3Spray bacterium, blower fan stirring simultaneously are 3 minutes in the aerosol cabinet, and post-sampling was air bacteria containing amount before the sterilization in 1 minute in static 1 minute, and (Candida albicans sprays medicine 3.9ml to the spray medicine; Staphylococcus aureus spray medicine 2.5ml), predetermined disinfecting time sampling 1 minute.Control group will spray medicine and change water spray into, and other is with the sterilization group.Carry out count plate with conventional method.
3. organic substance influence test: do the air sterillization test with the staphylococcus aureus bacteria suspension that contains 10% calf serum, observe organic matter this disinfectant air killing effect is had or not influence.
4. stability test: disinfectant is connected bottle put into 54 ℃ of water-baths 14 days, take out, by test method 2 test that carries out disinfection, the stability of observation disinfectant.
Above-mentioned test is obtained sterilizing rate (P) with following formula:
Pt: act on to the sterilizing rate of t time;
Nt: the natural decline rate of bacterium from 0 to t time the in the air;
Vo and Vt: be respectively before the experimental group sterilization and the airborne bacteria containing amount in sterilization back;
Vo ' and Vt ': be respectively in control group (corresponding to before the experimental group sterilization and the sterilization back) air
Bacteria containing amount.
Every kind of test repeats five times.
The result:
One. the neutralizer test: adopt dilution method to carry out the neutralization test result and show, spray medicine group and the no significant difference of control group bacterium amount prove that the disinfectant of aerosol cabinet does not have killing action (seeing Table 1) to the test organisms in the sampler.
The test of table 1 neutralizer
Sequence number control group spray medicine group
(cfu/ml)?????????????????(cfu/ml)
1??????????????????37000????????????????????27500
2??????????????????34000????????????????????39500
3??????????????????36500????????????????????50000
Average 35,830 39000
Two. bactericidal assay:
1. to the killing action of staphylococcus aureus in the air: at 1m
3Spray disinfectant 2.5ml is 99.99% (seeing Table 2) to 15,30 and 60 minutes killing rate of the staphylococcus aureus in the aerosol cabinet in the aerosol cabinet.
The killing effect of staphylococcus aureus in the table 2 pair air
Control group sterilization group
Disinfecting time is deposited viable count nature decline rate and is deposited the viable count killing rate
(branch) (cfu/m
3) (%) (cfu/m
3) (%)
0?????????????1.82×10
8???????????????????2.55×10
8
15????????????1.80×10
8??????1.10?????????1.88×10
4?????99.99
30????????????1.76×10
8??????3.30?????????3.75×10
3?????99.99
60????????????1.49×10
8??????18.13????????1.25×10
3?????99.99
2. to the killing action of Candida albicans in the air: at 1m
3Disinfectant 3.9ml is 99.91%, 99.96% and 100.00% (seeing Table 3) to 15,30 and 60 minutes killing rate of Candida albicans in the aerosol cabinet in the aerosol cabinet.
The killing effect of Candida albicans in the table 3 pair air
Control group sterilization group
Disinfecting time is deposited viable count nature decline rate and is deposited the viable count killing rate
(branch) (cfu/m
3) (%) (cfu/m
3) (%)
0????????????5.33×10
6????????????????????6.55×10
6
15???????????4.38×10
6??????17.82?????????5.00×10
3??????99.91
30???????????3.43×10
6??????35.65?????????1.70×10
3??????99.96
60???????????1.74×10
6??????67.36?????????0.00???????????100.00
Three. the organic substance influence test:
With 10% calf serum protective money staphylococcus aureus, the killing effect of this disinfectant there is not influence (seeing Table 4).
Table 4 organic matter is to Chinese herbal medicine air sanitizer killing effect influence test
The killing rate (%) of the different disinfecting time of serum content
(%) 5 minutes 15 minutes
0?????????????????99.99??????????????????99.99
10????????????????99.99??????????????????99.99
Annotate: not containing the preceding bacterium amount of calf serum group sterilization is 2.25 * 10
8Cfu/m
3
Containing the preceding bacterium amount of 10% calf serum group sterilization is 8.89 * 10
7Cfu/m
3
One, stability test
Result of the test shows: this disinfectant have good stability (seeing Table 5).
The stability test of table 5 Chinese herbal medicine air sanitizer
The killing rate (%) of different disinfecting time
5 minutes 15 minutes
Handle preceding 99.99 99.99
Handle back 99.99 99.99
Annotate: the bacterium amount is 2.25 * 10 before the control group sterilization
8Cfu/m
3
The bacterium amount is 8.40 * 10 before the experimental group sterilization
8Cfu/m
3
Test example 3 chicken embryo influenza virus sterilizing tests
Adopt national standard detection method (SOP), 1994, the 201-202 page or leaf carried out chicken embryo influenza virus sterilizing test.The results are shown in Table 6.
Table 6 Chinese herbal medicine air sanitizer is to the disinfective action of chicken embryo influenza virus
Disinfectant is measured papova dilution factor appeal inactivation ratio
10
-110
-210
-310
-410
-510
-610
-7CEID
50Deactivation index (%)
Chinese herbal medicine air test group stoste D
*/ 4 D/4 D/4 D/4
Disinfectant experimental group 1: 10 4
*/ 4 4/4 3/4 0/4 3.33 100 99
Experimental group 1: 20 4/4 4/4 4/4 4/4
Neutralizer control group 4/4 3/4 2/4 0/4 5.77
3/4?????3/4???????1/4???????0/4?????5.33
*Expression chicken embryo is all dead
*Expression chicken embryo infects number
1: 10 pair of A/ capital of Chinese herbal medicine air sanitizer section/30/95/ (H
3N
2) the appeal deactivation index of strain equals 100, the inactivation of virus rate equals 99%.
The anti-CoxB of test example 4 Chinese herbal medicine air sanitizers
3The virus test
Test method: test and the evaluation test result according to the SOP1994 of Shanghai City health and epidemic prevention station version " enterovirus micro plate cell culture neutralization test ".
The anti-CoxB of table 7 Chinese herbal medicine air sanitizer
3The virus test
The Chinese herbal medicine air sanitizer
1∶10????1∶20????1∶40????1∶80????1∶160????1∶320
The HeLa cell
*------
CoxB
3Virus
*---++ ++ ++ ++ ++ ++
(100TCID
50)
CoxB
3Virus control ++ ++
(100TCID
50)
The positive drug contrast-
(0.25% guanidine hydrochloride+CoxB
3)
The contrast of HeLa cell-
*Part toxicity
Conclusion: 1: 10 pair of HeLa cell of Chinese herbal medicine air sanitizer has mild toxicity, 1: 20 and above to HeLa cell avirulence.
Chinese herbal medicine air sanitizer 1: 20-1: 40 couples of CoxB
3Virus has complete inhibitory action
Remarks: CoxB
3Virus can cause myocarditis, respiratory tract acute infection etc.
The toxicology test of test example 5 Chinese herbal medicine air sanitizers
Test method is according to the GB7919-87 industrial toxicology
One, acute toxicity on inhalation test:
Quiet formula inhalation is adopted in experiment, and sample is with 100000mg/m
3Dosage animal is carried out heating evaporation contamination, contamination time 2 hours is observed a week, does not see that animal has the acute poisoning symptom and the phenomena of mortality, LD
50＞100000mg/m
3, the nontoxic level in true border.
Two, acute skin irritation test:
Sample is made the guinea pig skin irritant test with stoste, erythema and oedema do not occur, and the stimulation index average is 0, between 0～0.4, belongs to nonirritant by skin irritatin intensity evaluation highest score.
Three, mucomembranous irritant test:
Sample is made the lagophthalmos mucomembranous irritant test with stoste, and the average index of mucous membrane irritation is 0 after 48 hours, belongs to nonirritant.
Four, human body sensitization test (STT):
Sample carries out the human body sensitization test (STT) by GB7919-87, and totally 20, wherein 9 of men, 11 of woman at 22～66 years old age, do not have sensitization by this sample of its evaluation criterion to human body.
The report of application examples 1 Chinese herbal medicine air sanitizer field trial
Detect the total number of bacteria of 24 samples (restaurant private room air) according to Ministry of Public Health's disinfection technology standard.
Before the No.1-9 sterilization, after the preceding No.22-24 contrast of No.13-21 sterilization back No.10-12 contrast.
Method and step:
Thimerosal concentration (dosage): every cubic metre of 4 milliliters of stostes in space, 25 minutes action time
Test ambient temperature: (℃): 20 relative moisture (%): 68
The method of sampling: select 30m
3Four of private rooms, three intercropping sterilizing test groups, an intercropping control group, every of private room spray value is 120 milliliters, the control group spray is with the clear water of dosage.Before the sterilization nutrient agar panel is put in the room diagonal,, outer 3 points, highly all at 1.5 meters, exposes close immediately after 5 minutes lid as sterilization before air bacteria containing amount mensuration.Add the spraying of Chinese herbal medicine air sanitizer with wind and thunder II type ultra-low-volume sprayer then, treat 25 minutes after, other puts into the nutrient agar panel post-sampling (method is the same) that carries out disinfection.
Detection method: carry out total bacterial count with conventional method.
Testing result sees Table 8.
Table 8
The sterilization back is average before the sterilization
Average clump count (individual) killing rate (%) of the average clump count of detected object (individual)
Experimental group air (n=18) 5.22 0.22 95
Control group air (n=6) 5 4.67
Chinese herbal medicine air sanitizer Disinfection Effect of the present invention is an average kill ratio 95%.
The report of application examples 2 Chinese herbal medicine air sanitizers prevention infection of the upper respiratory tract practical application effect
Test is carried out (1999.1.14-1999.1.29) in the attached kindergarten of East China Normal University.Subjects is big (1), big (2), big (3), big (4) and big (5) class.The grouping situation:
The group number age
67 big (2) 34 5-6 years of experimental group
Greatly (3) 33
95 big (1) 30 5-6 years of control group
Greatly (4) 33
Greatly (5) 32
Method:
Control group 1: big (1) class---ultraviolet irradiation (1 on 40W lamp, 1 hour)+light-coloured vinegar stifling (500 gram) once a day.
Control group 2: big (4), big (5) class---ultraviolet irradiation (1 on 40W lamp, 1 hour) once a day.
Experimental group: the spraying of Chinese herbal medicine air sanitizer, every chamber 40ml, once a day.
Above-mentioned classroom area of disinfecting is 40m
2, high 3.5m.
The result:
(1) before the test: in the experimental group, big (3) class has 3 people to suffer from respiratory tract infection, and in the control group, big (1) class has 3 people, big (5) class to have 2 people to suffer from respiratory tract infection (seeing Table 9).
Table 9
| Experimental group | Control group | Add up to | |
| The morbidity number | ????3 | ????5 | ????8 |
| The number of not falling ill | ????64 | ????90 | ????154 |
| Add up to | ????67 | ????95 | ????162 |
X
2=0.222 X
2＜X
20.05, do not have significant difference between P＞0.05, two group.
(2) duration of test:
The infection of the upper respiratory tract (seeing Table 10A, 10B) does not appear in the experimental group.
In the control group, big (1) class (adopting ultraviolet ray+light-coloured vinegar to fumigate) has 11 people that the infection of the upper respiratory tract takes place, (4) class (employing ultraviolet irradiation) greatly, have 7 people that the infection of the upper respiratory tract takes place, big (5) classes (employing ultraviolet irradiation) have 9 people that the infection of the upper respiratory tract (seeing Table 10A, 10B) takes place.
Table 10A experimental group and the comparison of (1) class greatly
| Experimental group | Greatly (1) class | Add up to | |
| The morbidity number | ????0 | ???11 | ????11 |
| The number of not falling ill | ????67 | ???19 | ????86 |
| Add up to | ????67 | ???30 | ????97 |
X
2＝24.182??X
2＞X
20.001，P＜0.001。
Table 8B experimental group and big (4), the comparison of (5) class greatly
| Experimental group | (4) greatly, (5) class | Add up to | |
| The morbidity number | ????0 | ????16 | ????16 |
| The number of not falling ill | ????67 | ????49 | ????116 |
| Add up to | ????67 | ????65 | ????132 |
X
2＝16.527??X
2＞X
20.001，P＜0.001。
In entire test, the bad reaction that any use Chinese herbal medicine air cleaning disinfectant causes does not take place.
Conclusion: Chinese herbal medicine air sanitizer provided by the invention is safe and effective, and the effect of the tangible prevention infection of the upper respiratory tract is arranged, and it is stifling to be better than ultraviolet irradiation and ultraviolet ray+light-coloured vinegar.
Claims (7)
1. Chinese herbal medicine air sanitizer comprises:
Flos Lonicerae extractive solution 0.5-2%
Blumea oil 0.2-3%
2. Chinese herbal medicine air sanitizer as claimed in claim 1 wherein also comprises:
Propane diols 0-3%
The ethanol surplus
3. Chinese herbal medicine air sanitizer as claimed in claim 1 or 2 wherein also comprises lactic acid 0-3%.
4. the using method of the described Chinese herbal medicine air sanitizer of claim 1 is characterized in that: spray in people or nobody environment are arranged.
5. method as claimed in claim 4 wherein adopts sprayer to spray.
6. as claim 4 or 5 described methods, wherein the Chinese herbal medicine air sanitizer adopts stoste, and consumption is 0.5-2ml/m
3
7. as claim 4 or 5 described methods, wherein the Chinese herbal medicine air sanitizer adopts stoste dilution 2-3 doubly to spray the back.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031167004A CN1309305C (en) | 2003-04-29 | 2003-04-29 | Chinese herbal medicine air disinfectant |
| HK04109599A HK1066700A1 (en) | 2003-04-29 | 2004-12-03 | Traditional chinese medicine and herbs air disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031167004A CN1309305C (en) | 2003-04-29 | 2003-04-29 | Chinese herbal medicine air disinfectant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1541538A true CN1541538A (en) | 2004-11-03 |
| CN1309305C CN1309305C (en) | 2007-04-11 |
Family
ID=34320448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031167004A Expired - Lifetime CN1309305C (en) | 2003-04-29 | 2003-04-29 | Chinese herbal medicine air disinfectant |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN1309305C (en) |
| HK (1) | HK1066700A1 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2435421A (en) * | 2006-02-28 | 2007-08-29 | David Clayton Breeze | Method for disabling influenza virus |
| CN100364414C (en) * | 2006-02-06 | 2008-01-30 | 扬中牧乐药业有限公司 | Compound plant extract for preventing avian influenza and preparation thereof |
| CN101658190B (en) * | 2009-08-29 | 2011-11-30 | 夏焰明 | Liquid preparation with combination of vinegar and air-disinfecting traditional Chinese medicines |
| CN104488986A (en) * | 2014-12-26 | 2015-04-08 | 孔祥华 | Traditional Chinese medicine sterilizing liquid for clinical laboratory |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109874812A (en) * | 2019-04-17 | 2019-06-14 | 张宏睿 | A kind of clean operating room in hospital purificant |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1289542A (en) * | 1999-09-25 | 2001-04-04 | 铜陵市人民医院 | Air disinfectant and preparing process thereof |
| CN1345541A (en) * | 2000-09-29 | 2002-04-24 | 徐永安 | Sterilizing disinfectant |
-
2003
- 2003-04-29 CN CNB031167004A patent/CN1309305C/en not_active Expired - Lifetime
-
2004
- 2004-12-03 HK HK04109599A patent/HK1066700A1/en not_active IP Right Cessation
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100364414C (en) * | 2006-02-06 | 2008-01-30 | 扬中牧乐药业有限公司 | Compound plant extract for preventing avian influenza and preparation thereof |
| GB2435421A (en) * | 2006-02-28 | 2007-08-29 | David Clayton Breeze | Method for disabling influenza virus |
| CN101658190B (en) * | 2009-08-29 | 2011-11-30 | 夏焰明 | Liquid preparation with combination of vinegar and air-disinfecting traditional Chinese medicines |
| CN104488986A (en) * | 2014-12-26 | 2015-04-08 | 孔祥华 | Traditional Chinese medicine sterilizing liquid for clinical laboratory |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1309305C (en) | 2007-04-11 |
| HK1066700A1 (en) | 2005-04-01 |
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