CN107473327A - The method that Escherichia coli are removed using low temperature plasma - Google Patents
The method that Escherichia coli are removed using low temperature plasma Download PDFInfo
- Publication number
- CN107473327A CN107473327A CN201710960826.6A CN201710960826A CN107473327A CN 107473327 A CN107473327 A CN 107473327A CN 201710960826 A CN201710960826 A CN 201710960826A CN 107473327 A CN107473327 A CN 107473327A
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- CN
- China
- Prior art keywords
- escherichia coli
- low temperature
- temperature plasma
- nutrient medium
- fluid nutrient
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
Abstract
The invention discloses a kind of method that Escherichia coli are removed using low temperature plasma, it is characterised in that is vaccinated with to culture in the fluid nutrient medium of Escherichia coli, carries out exoelectrical reaction, reacted more than 9 15 minutes, you can all removes Escherichia coli.Operating method of the present invention is simple, and at normal temperatures, exoelectrical reaction can be obtained by good sterilization effect in the short time, while not produce poisonous and hazardous accessory substance, and equipment wide adaptation range.
Description
Technical field
A kind of sterilization processing the present invention relates to low temperature plasma to Escherichia coli, it is specifically a kind of to utilize medium
Inside is produced during barrier discharge and is rich in high chemically active electronics, ion, free radical and excited state molecule, in water body
The method killed of Escherichia coli.
Background technology
Water is the indispensable base substance of all life, and human health and drinking water quality are closely bound up safely.Due to life
State is constantly destroyed by the mankind, and environment increasingly deteriorates, and causes quality problem more and more, so as to threaten human health.World health
Tissue WHO statistics shows that 80% infectious disease is as caused by drinking water pollution mostly.Escherichia coli are as main, most normal
See, one of the bacterium that quantity is most, main parasitic is in human intestine.Escherichia coli are a kind of both ends blunt circle, whole body flagellum, energy
Motion, nonspore-bearing Gram-negative bacteria.Typically under normal circumstances, the perch in human body of most of Escherichia coli is not pathogenic,
Not only do not bring harm to human body, also with mutualism in human body.But once it enter gall-bladder, bladder may cause inflammation, one
Under fixed condition, extra intestinal infection can be caused.Part serological type strain is pathogenic strong, can cause diarrhoea in humans, stomachache, vomiting and
Heating.
Therefore, Escherichia coli are generally or by as pathogenic bacteria.Escherichia coli are chosen as the bacteriological inspection of the water hygiene and referred to
Mark, is one of important indicator of hygienic quality.In the case where sanitary condition is bad, Escherichia coli are dispersed in environment with excrement
In.Fresh-keeping foodstuff or domestic water detect Escherichia coli, and health can be caused a hidden trouble.Its presence may indicate that ring
Border (water body or food) is contaminated situation and degree, pollution level are substantially relevant with its quantity.Escherichia coli have been listed in water, food
Thing and medicine monitoring of hygiene index.Our national regulations, the total plate count in every milliliter of drinking water are less than 100, every 100 milliliters of water
In must not detect total coli group.
There is the method for many sterilizations at present.Chemical method, such as bacterium is killed with chemical agent, more often
That sees has chlorhexidine-containing disinfectant, and hypochlorous acid is generated after mainly being combined using chlorine and water, destroys the activity and nucleic acid knot of desmoenzyme
Structure, until bacterial death.Although bactericidal effect is pretty good, the accessory substance of generation is mostly chloride to cause secondary pollution to environment.
Ultraviolet disinfection is also one of relatively conventional sterilization method, although this method can solve the problems, such as harmful by-products,
It is also not satisfactory that disinfecting process takes longer and effect.
In the last few years, flourishing with technology, low temperature plasma is as a new technique in many application sides
Face offers convenience to the mankind.Such as the field such as space flight, environment.Lower temperature plasma technology safely, conveniently, non-residual toxicity be
Sterilization provides a kind of new possibility.
Low temperature plasma is the 4th state beyond solid, liquid, gas tri-state material.Put when additional magnitude of voltage reaches gas
Can be breakdown during piezoelectric voltage, produce the mixtures such as electronics, ion, free radical.Because low temperature is presented in whole system in discharge process,
So it is referred to as low temperature plasma.General principle is to utilize (such as ozone, the oxygen such as these caused active particle, free radicals
Atom, hydroxyl radical free radical) Escherichia coli are killed.Because caused active material all has very strong activity, to bacterium
There is extremely strong oxidation, penetrate into bacteria wall and enter inside, for example organize after birth, invade in cell membrane and act on outer membrane fat egg
White and internal lipopolysaccharides, is distorted the cell of bacterium, finally causes cell dissolving dead.Meanwhile produced in discharge process
Raw ultraviolet light also functions to certain effect to sterilization, however, experimentation have shown that, also it is due to mainly high energy particle and Mars free radical
Effect cause the final death of Escherichia coli.
The content of the invention
Problem to be solved by this invention is to provide a kind of simple to operate, high treating effect and does not produce harmful by-products
Lower temperature plasma technology kill Escherichia coli method.
In order to solve the above problems, the present invention is achieved through the following technical solutions：One kind is gone using low temperature plasma
Except the method for Escherichia coli, it is characterised in that it is vaccinated with to culture in the fluid nutrient medium of Escherichia coli, carries out exoelectrical reaction,
It is more than minute to react 9-15, you can all remove Escherichia coli.
Preferably, during the course of the reaction, air is passed through, then Escherichia coli can all be removed by reacting 9 minutes.
It is highly preferred that the air velocity that is passed through is 0.6L/min.
Preferably, the condition of culture for cultivating the fluid nutrient medium for being vaccinated with Escherichia coli is to cultivate 18h at 37 DEG C.
Preferably, the reacting dose of the Escherichia coli is 3mL.
Preferably, the pH value of the fluid nutrient medium is 6.
Preferably, the input voltage of the exoelectrical reaction is 45~65v, now output voltage peak value be 12~
16kV。
Compared with prior art, beneficial effects of the present invention are as follows：
1) technique is simple, and operating method is easy：Only process object need to be put into reactor, be adjusted to corresponding input voltage i.e.
Can；
2) wide adaptation range：In -60 DEG C to+450 DEG C of environment can normal operation, it is molten containing flue gas, dust, gas
Can normal operation under the environment such as glue, steam.Service life of equipment is longer；
3) harmful by-products are not produced：Process gas discharge will not while always caused free radical is killed to Escherichia coli
Produce poisonous and harmful substance secondary pollution environment；
4) processing time is shorter：Compare with the conventional method of some sterilizations, this technology can be in the short period of time
Effectively Escherichia coli are carried out with 100% killing.
Brief description of the drawings
Fig. 1 be the Escherichia coli bacteria liquid that low temperature plasma is 6 to initial pH value be respectively 1 in discharge time, 2,5,8,
9th, 10,15,20,30 minutes when clearance figure；
Fig. 2 be the Escherichia coli bacteria liquid that low temperature plasma is 6 to initial pH value be respectively 1 in discharge time, 2,5,8,
9th, 10,15,20,30 minutes when, while clearance figure when being continually fed into 0.6L/min air；
Fig. 3 be the Escherichia coli bacteria liquid that low temperature plasma is 6 to initial pH value be respectively 1 in discharge time, 2,5,8,
9th, 10,15,20,30 minutes when, clearance figure when input voltage is respectively 65v, 55v, 45v；
Fig. 4 be low temperature plasma be 6 to initial pH value Escherichia coli bacteria liquid electric discharge input voltage be respectively 65v,
When 55v, 45v, the growth curve chart after renewed vaccination.
Embodiment
To become apparent the present invention, hereby with preferred embodiment, and accompanying drawing is coordinated to be described in detail below.
Embodiment 1
A kind of method that Escherichia coli are removed using low temperature plasma：
The previous day configuration Escherichia coli bacteria liquid is carried, inoculation 18h, pH value 6 are cultivated at 37 DEG C (220rpm).3ml is taken every other day
Bacterium solution is put into reactor and reacted.Power supply and reactor is linked in sequence, adjustment input voltage is 65v, proceeds by the time
The plasma discharge reaction of respectively 1,2,5,8,9,10,15,20,30 minute.The clearance of Escherichia coli in different time
See Fig. 1.The growth over time of the clearance of Escherichia coli and increase.At the 10th minute, clearance reached 99.90%；
At the 15th minute, clearance reaches 100%.
Embodiment 2
A kind of method that Escherichia coli are removed using low temperature plasma：
Escherichia coli bacteria liquid is configured according to the operating procedure of embodiment 1, takes 3ml bacterium solutions to be put into reactor every other day and carries out instead
Should.Power supply and reactor is linked in sequence, adjustment input voltage is 65v, proceed by the time be respectively 1,2,5,8,9,10,
15th, the plasma discharge reaction of 20,30 minutes, while it is continually fed into 0.6L/min air.Escherichia coli in different time
Clearance see Fig. 2.The growth over time of the clearance of Escherichia coli and increase.At the 8th minute, clearance reached
99.50%；At the 10th minute, clearance reached 100%.
Embodiment 3
A kind of method that Escherichia coli are removed using low temperature plasma：
Escherichia coli bacteria liquid is configured according to the operating procedure of embodiment 1, takes 3ml bacterium solutions to be put into reactor every other day and carries out instead
Should.Power supply and reactor is linked in sequence, proceeds by the plasma that the time is respectively 1,2,5,8,9,10,15,20,30 minute
Body exoelectrical reaction, and it is 65v, 55v, 45v to change successively and adjust input voltage.Corresponding different input voltages at different moments
Fig. 3 is shown in the clearance of Escherichia coli.When the time is constant, the clearance of Escherichia coli increases as voltage raises.With 15
Exemplified by minute, when input voltage is 45v, clearance only has 23.07；During 55v, clearance reaches 56.55%；During 65v, clearance
Reach 100%.
Embodiment 4
A kind of method that Escherichia coli are removed using low temperature plasma：
Escherichia coli bacteria liquid is configured according to the operating procedure of embodiment 1, takes 3ml bacterium solutions to be put into reactor every other day and carries out instead
Should.Power supply and reactor is linked in sequence, it is 65v, 55v, 45v to change successively and adjust input voltage, and the reaction time is 15 points
Clock.Negate the measure that the bacterium solution that should terminate carries out OD600 values, these point-renderings are into corresponding growth curve.Different input voltages
Escherichia coli Growth curve after corresponding in connecing again is shown in Fig. 4.Control is constant at 15 minutes between upon discharging, and input voltage is got over
Height, the trend that Escherichia coli breed again is more obvious, growth it is faster.When input voltage is 65v, Escherichia coli are all dead
Die, just not regrowth.
Claims (7)
- A kind of 1. method that Escherichia coli are removed using low temperature plasma, it is characterised in that be vaccinated with Escherichia coli to culture Fluid nutrient medium in, carry out exoelectrical reaction, reaction 9-15 more than minute, you can all remove Escherichia coli.
- 2. the method according to claim 1 that Escherichia coli are removed using low temperature plasma, it is characterised in that reacting During, air is passed through, then Escherichia coli can all be removed by reacting 9 minutes.
- 3. the method according to claim 2 that Escherichia coli are removed using low temperature plasma, it is characterised in that described logical It is 0.6L/min to enter air velocity.
- 4. the method according to claim 1 that Escherichia coli are removed using low temperature plasma, it is characterised in that the training The condition of culture for supporting the fluid nutrient medium for being vaccinated with Escherichia coli is to cultivate 18h at 37 DEG C.
- 5. the method that Escherichia coli are removed using low temperature plasma according to claim 1 or 4, it is characterised in that institute The reacting dose for stating Escherichia coli is 3mL.
- 6. the method that Escherichia coli are removed using low temperature plasma according to claim 1 or 4, it is characterised in that institute The pH value for stating fluid nutrient medium is 6.
- 7. the method according to claim 1 that Escherichia coli in water body are removed using low temperature plasma, it is characterised in that The input voltage of the exoelectrical reaction is 45~65v, and now output voltage peak value is 12~16kV.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077904A (en) * | 2017-12-21 | 2018-05-29 | 山西佰富康生物科技有限公司 | A kind of Cordyceps militaris nutrition brewed powder and preparation method thereof |
| CN114903056A (en) * | 2022-04-18 | 2022-08-16 | 浙江大学 | Method for removing biofilm by using phage before low-temperature plasma activation |
| CN115104534A (en) * | 2022-07-19 | 2022-09-27 | 赣南师范大学 | Non-damage physical disinfection and separation method after plant callus contamination |
Citations (3)
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| CN87105077A (en) * | 1987-09-04 | 1988-04-06 | 鞍山市静电技术研究所 | Process for sterilization of electrical fluid effect and device |
| CN101537198A (en) * | 2009-04-16 | 2009-09-23 | 西安交通大学 | Killing method of colibacillus |
| CN104591355A (en) * | 2015-01-29 | 2015-05-06 | 广东佳德环保科技有限公司 | Method for disinfecting and sterilizing water by virtue of streamer discharge plasmas |
-
2017
- 2017-10-16 CN CN201710960826.6A patent/CN107473327A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87105077A (en) * | 1987-09-04 | 1988-04-06 | 鞍山市静电技术研究所 | Process for sterilization of electrical fluid effect and device |
| CN101537198A (en) * | 2009-04-16 | 2009-09-23 | 西安交通大学 | Killing method of colibacillus |
| CN104591355A (en) * | 2015-01-29 | 2015-05-06 | 广东佳德环保科技有限公司 | Method for disinfecting and sterilizing water by virtue of streamer discharge plasmas |
Non-Patent Citations (1)
| Title |
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| 郝学安等: "《现代医院感染控制与实用消毒技术》", 31 July 2009 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077904A (en) * | 2017-12-21 | 2018-05-29 | 山西佰富康生物科技有限公司 | A kind of Cordyceps militaris nutrition brewed powder and preparation method thereof |
| CN114903056A (en) * | 2022-04-18 | 2022-08-16 | 浙江大学 | Method for removing biofilm by using phage before low-temperature plasma activation |
| CN115104534A (en) * | 2022-07-19 | 2022-09-27 | 赣南师范大学 | Non-damage physical disinfection and separation method after plant callus contamination |
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Application publication date: 20171215 |
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