CN1540319A - Method for testing peroxide value of liquid edible oil - Google Patents
Method for testing peroxide value of liquid edible oil Download PDFInfo
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- CN1540319A CN1540319A CNA031143628A CN03114362A CN1540319A CN 1540319 A CN1540319 A CN 1540319A CN A031143628 A CNA031143628 A CN A031143628A CN 03114362 A CN03114362 A CN 03114362A CN 1540319 A CN1540319 A CN 1540319A
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- China
- Prior art keywords
- peroxide value
- diaphragm
- pastille
- molecular
- agent
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Abstract
Prepared tablet containing sensitive layer is utilized in the invention. The said layer includes reducing agent, color-developing agent, stabilizing agent and reaction accelerating agent etc. dripping sample of liquid edible oil to be tested into tablet containing sensitive layer can semi-quantificationally determine range of peroxide value of the sample through visualization based on color table. Peroxide value can be determined with accuracy by using visible light analyzer in sensitivity 0.01meq/kg, measuring range 0-50meq/kg and testing time 120 seconds.
Description
Technical field the present invention relates to a kind of method of peroxide value of fast measuring liquid edible oil.
The detection of background technology edible oil peroxide value is a key means of guaranteeing oil quality, and domestic and international widely used assay method mainly contains high performance liquid chromatography and chemical titration.High-efficient liquid phase technique need use large-scale instrument, and the reagent cost height is difficult to use as generaI investigation; And the reaction time of chemical titration is long, complex steps, and reagent need be proposed above preparation the last week, and is all hand-manipulated, is subject to operating personnel's interference from human factor.The analytical approach (chemical titration) that the edible oil peroxide value is all stipulated according to GB/T5009.37-1996 detects in each epidemic prevention department of China at present.Delivered both at home and abroad the patent of invention document that detects superoxide in succession, related to test paper as Chinese patent (CN87106131.7) " test paper for determining peroxy-acetic acid and preparation method thereof " and measure Peracetic acid content in the water-soluble thimerosal; Jap.P. (clear 53-30383) " superoxide is measured the preparation method of test paper in the grease " and Chinese patent (CN1111015A) " detection test paper of peroxide level and preparation method thereof in edible oil and the oil-containing food ", be as principal ingredient with soluble starch, potassium iodide, all has following defective:, easily lost efficacy to light and oxygen sensitivity; Reaction time was longer, above 5 minutes; Can only qualitatively measure, can not measure accurate numerical value etc. with sxemiquantitative.The objective of the invention is to propose a kind of detection method, use previously prepared pastille diaphragm, be used, can quantitatively measure the peroxide value of liquid edible oil with the visible light analysis instrument; When being used, just can semiquantitatively measure the peroxide value scope with the range estimation mode with colorimetric scale.Compared with prior art, outstanding feature of the present invention has: 1. can estimate, also can be used with instrument; 2. estimate or be used, can accomplish real-time, on-the-spot quality monitoring with portable visible light analysis instrument; 3. the diaphragm contained drug is stable, and the holding time is long; 4. the reaction time lacks, and sample does not need pre-service, and only need 120 seconds the detection time of each sample.
Summary of the invention 1. these methods are prepared into the pastille diaphragm by absorption medicine in the high-molecular bone shelf structure and dry.When liquid edible oil samples contact diaphragm, contained drug reacts in superoxide in the oil samples and the diaphragm, manifests color, and the relation of the degree of change color and the peroxide value of oil samples can be expressed with quadratic equation.The reflectivity that detects diaphragm in visible-range can accurately be measured peroxide value.Also can be used with colorimetric scale, the scope of peroxide value is judged in range estimation.
2. the high-molecular bone shelf structure can use nylon miillpore filter or filter paper.
3. the medicine that contains in the diaphragm is made of citric acid buffer reagent, stabilizing agent, developer, reductive agent and reaction promoter etc.Wherein, stabilizing agent is the multipolymer of ethylene methacrylic ester and maleic anhydride, molecular weight ranges 900000～1300000; Developer is the derivant of biphenylamine, and molecular formula is
[R
1C
6H
mNR
2]
2 m＝2-4
R
1＝H，CH
3，C
6H
5，CH
3O，NH
2
R
2＝H，CH
3；
Reductive agent is a peroxidase; Reaction promoter is the derivant of benzothiazole, and molecular formula is
R
3＝H，CH
3，C
2H
5
R
4＝H，OH，SH，NH
2。
4. the preparation of pastille diaphragm, carry out according to the following steps:
1) citric acid buffer reagent, reductive agent and stabilizing agent are dissolved in the deionized water, obtain solution A;
2) developer and reaction promoter are dissolved in the absolute ethyl alcohol, obtain solution B;
3) filter membrane or filter paper are soaked in solution A and solution B successively and in 50～70 ℃ of following forced air dryings of temperature.
4) dry good diaphragm is cut, and is encapsulated in the vacuum aluminium foil bag or is equipped with in the plastic bottle of silica-gel desiccant.
5. the wavelength coverage 600～650mm of detection of reflected rate, preferably 630nm.
6. the making of typical curve: prepare peroxide value titers at different levels (0～50meq/kg), demarcate actual value with high-efficient liquid phase technique or chemical titration.On diaphragm, quantitatively drip titer, detect the reflectivity of diaphragm after 120 seconds at selected wavelength, make reflectivity---peroxide value relation curve and standard equation.
7. the making of visual colorimetry table:, the diaphragm that presents different colours as standard color block, is made colorimetric scale with the standard color block basically identical with four-color process with peroxide value titer and iris action.
8. using method:
1) detection by quantitative: on the pastille diaphragm, drip sample, measure the reflectivity of diaphragm after 120 seconds in wavelength selected, and calculate the peroxide value of sample according to standard equation.
2) half-quantitative detection (range estimation): on the pastille diaphragm, drip sample or diaphragm immersed in the sample 1～2 second and took out and keep flat, after 120 seconds the color that presents on the diaphragm is compared the peroxide value of judgement sample with the colorimetric scale range estimation.
Embodiment 1. is dissolved in 200 gram citric acid buffer reagents, 30000 unit horseradish peroxidases and 30 gram stabilizing agents in 1000 ml deionized water successively.Nylon 66 miillpore filters are immersed in the solution, take out 60 ℃ of oven dry after 20 seconds; Again 10 gram developers, 20 gram reaction promoters are dissolved in 1000 milliliters of absolute ethyl alcohols, filter membrane was soaked in solution 29 seconds, take out and, cut into the circular film of 10 millimeters of diameters, be divided in the aluminium foil bag that airtight vacuum packs stand-by 60 ℃ of oven dry.
2. drip the sample of an oil to be measured with suction pipe at the pastille diaphragm, at the reflectivity of wavelength 630 nanometer detection diaphragms, calculate the peroxide value of sample according to standard equation or relation curve after 120 seconds.
3. diaphragm was immersed in the oil samples to be measured 1～2 second, take out and kept flat 120 seconds, the color of diaphragm is compared with colorimetric scale, select the immediate color lump of color, promptly represented the peroxide value scope of sample.
Claims (10)
- One, a kind of preparation method who is used to measure the pastille diaphragm of liquid edible oil peroxide value.
- Two, cooperate the method for quantitative measurement peroxide value with the visible light analysis instrument with the pastille diaphragm in the claim one.
- Three, cooperate the method for semiquantitative determination peroxide value scope with colorimetric scale with the pastille diaphragm in the claim one.
- Four, the described pastille diaphragm of claim one contains citric acid buffer agent, reductive agent, stabilizing agent, developer, reaction promoter and high-molecular bone shelf structure.
- Five, the described reductive agent of claim four is a peroxidase.
- Six, the described stabilizing agent of claim four is the multipolymer of ethylene methacrylic ester and maleic anhydride, molecular weight ranges 900000～1300000.
- Seven, the described developer of claim four is the derivant of biphenylamine, molecular formula[R 1C 6H mNR 2] 2 m＝2-4R 1＝H 2，CH 3，C 6H 5，CH 3O，NH 2R 2＝H，CH 3
- Eight, the described reaction promoter of claim four is the derivant of benzothiazole, molecular structurem＝3-4R 3＝H，CH 3，C 2H 5R 4＝H，OH，SH，NH 2
- Nine, the described high-molecular bone shelf structure of claim four is nylon miillpore filter or filter paper.
- Ten, the described visible light analysis use of claim two wavelength is 600～650nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031143628A CN1540319A (en) | 2003-04-27 | 2003-04-27 | Method for testing peroxide value of liquid edible oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031143628A CN1540319A (en) | 2003-04-27 | 2003-04-27 | Method for testing peroxide value of liquid edible oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1540319A true CN1540319A (en) | 2004-10-27 |
Family
ID=34320191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031143628A Pending CN1540319A (en) | 2003-04-27 | 2003-04-27 | Method for testing peroxide value of liquid edible oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1540319A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102706872A (en) * | 2012-06-13 | 2012-10-03 | 安徽省农业科学院农产品加工研究所 | Combined reagent for detecting peroxide value of edible oil and fat and detection method thereof |
| CN103245660A (en) * | 2013-05-07 | 2013-08-14 | 浙江大学 | Edible oil peroxide value measurement combined reagent and detection method |
| CN104483314A (en) * | 2014-12-25 | 2015-04-01 | 桂林中辉科技发展有限公司 | Method for improvingthermal stability of test strips for detecting peroxide value of cooking oil |
| CN105241846A (en) * | 2014-07-11 | 2016-01-13 | 马井芳 | Method of detecting peroxide value of liquid-phase edible oil |
| CN106383119A (en) * | 2016-11-08 | 2017-02-08 | 自贡勃生表面技术推广有限公司 | Spectrophotometry of rapidly detecting concentration of peroxide in edible oil |
-
2003
- 2003-04-27 CN CNA031143628A patent/CN1540319A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102706872A (en) * | 2012-06-13 | 2012-10-03 | 安徽省农业科学院农产品加工研究所 | Combined reagent for detecting peroxide value of edible oil and fat and detection method thereof |
| CN103245660A (en) * | 2013-05-07 | 2013-08-14 | 浙江大学 | Edible oil peroxide value measurement combined reagent and detection method |
| CN103245660B (en) * | 2013-05-07 | 2015-09-30 | 浙江大学 | A kind of edible oil and fat determination of POV composite reagent and detection method |
| CN105241846A (en) * | 2014-07-11 | 2016-01-13 | 马井芳 | Method of detecting peroxide value of liquid-phase edible oil |
| CN104483314A (en) * | 2014-12-25 | 2015-04-01 | 桂林中辉科技发展有限公司 | Method for improvingthermal stability of test strips for detecting peroxide value of cooking oil |
| CN106383119A (en) * | 2016-11-08 | 2017-02-08 | 自贡勃生表面技术推广有限公司 | Spectrophotometry of rapidly detecting concentration of peroxide in edible oil |
| CN106383119B (en) * | 2016-11-08 | 2019-01-01 | 自贡勃生表面技术推广有限公司 | Quickly detect the photometry of peroxide concentrations in edible oil |
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