CN103245660A - Edible oil peroxide value measurement combined reagent and detection method - Google Patents
Edible oil peroxide value measurement combined reagent and detection method Download PDFInfo
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Abstract
本发明涉及过氧化值的测定,尤其涉及一种食用油脂过氧化值测定组合试剂及检测方法,包括:极性有机溶剂、还原剂、显色试剂、显色稳定剂;所述极性有机溶剂按质量百分比记,包括乙醇的质量浓度为237~710g/L;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为1~20g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为300~1000g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为20~800g/L。本发明采用比色分析的方法,操作迅速简便,减少了加热对检测结果的干扰;所用检测试剂种类较少,便于装配携带;可同时对多个油脂样本进行显色和检测,显色均匀;所用检测试剂均低毒甚至无毒,安全环保。The present invention relates to the determination of the peroxide value, in particular to a combination reagent and detection method for the determination of the peroxide value of edible oils and fats, comprising: a polar organic solvent, a reducing agent, a color developing reagent, and a color developing stabilizer; the polar organic solvent Recorded by mass percentage, the mass concentration of ethanol is 237-710g/L; the reducing agent is recorded by weight percentage, and the mass concentration of ferrous sulfate is 1-20g/L; the color reagent is sulfosalicyl acid solution, the mass concentration is 300-1000g/L; the color stabilizer is pyridoxamine hydrochloride solution, the mass concentration is 20-800g/L. The invention adopts the method of colorimetric analysis, which is quick and easy to operate, and reduces the interference of heating on the detection results; the detection reagents used are less in types, which is convenient for assembly and carrying; the color development and detection of multiple oil samples can be carried out at the same time, and the color development is uniform; The detection reagents used are all low-toxic or even non-toxic, safe and environmentally friendly.
Description
技术领域 technical field
本发明涉及过氧化值的测定,尤其涉及一种食用油脂过氧化值测定组合试剂及检测方法。 The invention relates to the measurement of peroxide value, in particular to a combination reagent and detection method for the determination of peroxide value of edible oils and fats. the
背景技术 Background technique
众所周知,食用油脂或含油食品,从生产到消费需要一定时间,有时需要长期贮藏,而在贮存或使用期间,油脂或含油食品易受空气中的氧气、光照等的影响而进行以氧化为主体的反应,不仅在感官上发生变化,如色泽加深、产生令人不快气味(主要是生成的过氧化物易部分分解形成的醛类物质引起的),而且会使营养劣变,劣变程度严重甚至会产生有毒物质,威胁到消费者的身体健康。 As we all know, edible oily or oily food needs a certain period of time from production to consumption, and sometimes requires long-term storage. During storage or use, oily or oily food is easily affected by oxygen in the air, light, etc., and undergoes oxidation as the main body. The reaction will not only change the senses, such as deepening the color and producing unpleasant odors (mainly caused by the aldehydes formed by the easy partial decomposition of the generated peroxides), but also will cause nutritional deterioration, the degree of deterioration is serious or even Toxic substances will be produced, threatening the health of consumers. the
目前以油脂氧化产物为检测对象的方法主要有以下这些: At present, the main methods for detecting oil oxidation products are as follows:
碘量法是过氧化物检测的标准方法,其原理是在酸性介质中,过氧化物与碘离子反应生成单质碘,再用硫代硫酸钠标准溶液滴定析出的碘,并根据消耗硫代硫酸钠标准溶液的体积来计算油脂的过氧化值。此法操作繁琐,灵敏度低,取样量大,检测费用高,仅适用于含0.2mEq/kg以上过氧化物的测定,同时易对环境造成污染。 Iodometric method is a standard method for peroxide detection. Its principle is that in acidic medium, peroxide reacts with iodide ions to generate elemental iodine, and then titrates the precipitated iodine with sodium thiosulfate standard solution, and according to the consumption of thiosulfuric acid The volume of sodium standard solution was used to calculate the peroxide value of oil. This method is cumbersome to operate, has low sensitivity, large sampling volume, and high detection cost. It is only suitable for the determination of peroxides containing more than 0.2mEq/kg, and it is easy to cause pollution to the environment. the
硫代巴比妥酸法是实验室常用的油脂氧化检测的方法,此方法已有检测试纸的专利(专利号JP51151191)。丙二醛是油脂氧化产生的诸多不饱和醛酮产物中主要产物之一,它与硫代巴比妥酸生成的红色化合物,在530nm处有最大吸收,测定有色物的吸光度值,以此来衡量油脂的氧化程度。该方法的缺点主要为:因加热使检测结果有误差。 The thiobarbituric acid method is a method commonly used in laboratories to detect oil oxidation. This method has a patent for detection test paper (Patent No. JP51151191). Malondialdehyde is one of the main products of many unsaturated aldehydes and ketones produced by oil oxidation. The red compound formed by it and thiobarbituric acid has a maximum absorption at 530nm, and the absorbance value of the colored substance is measured to determine Measures the degree of oxidation of fats and oils. The main disadvantage of this method is that the detection result has errors due to heating. the
硫氰酸铁法,它的原理是在酸性条件下二价铁离子可被过氧化物氧化成三价铁离子,当加入硫氰酸根离子时,则与铁离子形成橙红色的络合物,在480-515 nm内有最大吸收值,通过比色即可测出过氧化物的含量。但该方法中,甲醇混合试剂溶解油脂样品,有害于人体且污染环境。 Ferric thiocyanate method, its principle is that ferrous ions can be oxidized to ferric ions by peroxide under acidic conditions, and when thiocyanate ions are added, an orange-red complex is formed with ferric ions. There is a maximum absorption value within 480-515 nm, and the content of peroxide can be measured by colorimetry. However, in this method, the methanol mixed reagent dissolves the oil sample, which is harmful to the human body and pollutes the environment. the
一种基于电导率的食用油过氧化值测定方法(专利申请号CN201210324117.6),其原理是称取一定量的食用油样品,加入一定量异辛烷和浓盐酸,混合均匀,加入饱和碘化钾溶液,振荡后,立即加入一定量蒸馏水稀释一定比例,振荡后静置时间,用电导率仪测定混合水相的电导率值。这类方法对操作人员要求高,同时设备复杂、成本过高,不利于推广使用。 A method for measuring the peroxide value of edible oil based on electrical conductivity (patent application number CN201210324117.6), the principle of which is to weigh a certain amount of edible oil sample, add a certain amount of isooctane and concentrated hydrochloric acid, mix well, add saturated potassium iodide Solution, after shaking, immediately add a certain amount of distilled water to dilute a certain ratio, let stand for a while after shaking, and measure the conductivity value of the mixed water phase with a conductivity meter. This type of method has high requirements for operators, and at the same time, the equipment is complicated and the cost is too high, which is not conducive to popularization and use. the
综上所述,各种检测方法的灵敏度、精确度、检测简繁程度和成本等方面均存在较大差异,因而建立新的简便易行、灵敏度高,准确性好,检测条件温和,成本低廉,耗时少的绿色检测方法仍是社会的迫切要求和重要的研究目标。 To sum up, there are big differences in the sensitivity, accuracy, detection simplicity and cost of various detection methods, so it is easy to establish a new detection method with high sensitivity, good accuracy, mild detection conditions and low cost. , a green detection method with less time consumption is still an urgent requirement of the society and an important research goal. the
发明内容 Contents of the invention
本发明为克服上述的不足之处,目的在于提供一种食用油脂过氧化值测定组合试剂,组合试剂的使用简单易行、灵敏度高,大大降低成本,对人体无公害。 In order to overcome the above disadvantages, the present invention aims to provide a combined reagent for measuring the peroxide value of edible oils and fats. The combined reagent is simple and easy to use, has high sensitivity, greatly reduces cost, and has no pollution to human body. the
本发明的另一目的在于提供一种食用油脂过氧化值测定方法,基于对色法原理,通过组合试剂对待测样品的过氧化值进行检测,操作简便快捷,准确率高,又环保安全。 Another object of the present invention is to provide a method for measuring the peroxide value of edible oils and fats. Based on the principle of colorimetry, the peroxide value of the sample to be tested is detected by a combination of reagents. The operation is simple and fast, the accuracy is high, and it is environmentally friendly and safe. the
本发明是通过以下技术方案达到上述目的:一种食用油脂过氧化值测定组合试剂,包括:极性有机溶剂、还原剂、显色试剂、显色稳定剂; The present invention achieves the above object through the following technical solutions: a combination reagent for measuring the peroxide value of edible oils and fats, comprising: a polar organic solvent, a reducing agent, a color developing reagent, and a color developing stabilizer;
所述极性有机溶剂按质量百分比记,包括乙醇的质量浓度为237~710g/L; The polar organic solvent is recorded by mass percentage, and the mass concentration including ethanol is 237~710g/L;
所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为1~20g/L; The reducing agent is recorded by weight percentage, including the mass concentration of ferrous sulfate is 1 ~ 20g/L;
所述显色试剂为磺基水杨酸溶液,质量浓度为300~1000 g/L; The chromogenic reagent is a sulfosalicylic acid solution with a mass concentration of 300-1000 g/L;
所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为20~800 g/L。 The color stabilizer is pyridoxamine hydrochloride solution with a mass concentration of 20-800 g/L. the
一种食用油脂过氧化值测定方法,包括以下步骤: A method for measuring the peroxide value of edible oils and fats, comprising the following steps:
(1)用移液枪移取液态待测样品,所述的待测样品的取样体积为20~40μL,同时记录下质量,加入极性有机溶剂和显色稳定剂的混合液,所述待测样品与极性有机溶剂的体积比为1:15~1:25,极性有机溶剂与显色稳定剂的体积比为 1:30~1:40,涡流震荡; (1) Pipette the liquid sample to be tested with a pipette gun. The sampling volume of the sample to be tested is 20-40 μL. At the same time, record the mass, add a mixture of polar organic solvent and color stabilizer, and The volume ratio of the test sample to the polar organic solvent is 1:15~1:25, the volume ratio of the polar organic solvent to the color stabilizer is 1:30~1:40, vortex oscillation;
(2)在步骤(1)的混合液中加入还原剂,每克待测样品滴加的还原剂体积为5~12mL,涡流震荡,室温下静置; (2) Add a reducing agent to the mixed solution in step (1), the volume of the reducing agent added dropwise per gram of the sample to be tested is 5-12mL, vortex and shake, and stand at room temperature;
(3)在步骤(2)的溶液中加入显色剂,每克待测样品滴加的显色剂体积为0.1~2mL,溶液以10000r/min以上的转速离心旋转10分钟; (3) Add a color developer to the solution in step (2), the volume of the color developer added dropwise per gram of the sample to be tested is 0.1-2mL, and the solution is centrifuged at a speed above 10000r/min for 10 minutes;
(4)取步骤(3)得到混合溶液的上清部分在500~550nm处测定吸光度值; (4) Take the supernatant part of the mixed solution obtained in step (3) and measure the absorbance value at 500-550 nm;
(5)根据吸光度值从铁离子标准曲线图上得到对应的铁离子浓度,并换算成单位质量的铁离子浓度作为过氧化值。 (5) Obtain the corresponding iron ion concentration from the iron ion standard curve according to the absorbance value, and convert it into the iron ion concentration per unit mass as the peroxide value. the
作为优选,所述待测样品为液态。 Preferably, the sample to be tested is in a liquid state. the
所述铁离子标准曲线图的制作包括以下步骤: The making of described iron ion standard curve diagram comprises the following steps:
(a)将还原铁粉加入盐酸和过氧化氢溶解后,加热除去过氧化氢,冷却后用水稀释混匀,制得10mg/mL的铁标准储备液; (a) After adding the reduced iron powder into hydrochloric acid and hydrogen peroxide to dissolve, heat to remove the hydrogen peroxide, dilute and mix with water after cooling to obtain a 10mg/mL iron standard stock solution;
(b)利用步骤(a)制得的溶液以蒸馏水稀释的方法得到铁离子浓度为0、0.2、0.5、1、1.5、2.0、2.5、3.0、3.5、4.0 mg/mL的一系列梯度溶液,分别加入所述极性溶剂和显色稳定剂后震荡30秒; (b) Dilute the solution prepared in step (a) with distilled water to obtain a series of gradient solutions with iron ion concentrations of 0, 0.2, 0.5, 1, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0 mg/mL, Shake for 30 seconds after adding the polar solvent and color stabilizer respectively;
(c)在步骤(b)得到的各溶液中加入还原剂后震荡30秒反应3分钟,再加入显色剂; (c) Add reducing agent to each solution obtained in step (b), shake for 30 seconds and react for 3 minutes, then add color developing agent;
(d)将步骤(c)得到的各溶液以10000r/min以上的转速离心10分钟,以铁离子浓度为0的反应管为参照在500~550nm处测定吸光度值; (d) Centrifuge each solution obtained in step (c) at a speed of 10,000 r/min or more for 10 minutes, and measure the absorbance value at 500-550 nm with reference to the reaction tube with an iron ion concentration of 0;
(e)分别以吸光度值、铁离子浓度为横、纵坐标绘制曲线,并用最小二乘法得到拟合的直线方程。 (e) Draw a curve with the absorbance value and iron ion concentration as the abscissa and ordinate, and use the least square method to obtain the fitted straight line equation. the
本发明的有益效果在于:本发明采用比色分析的方法,油脂样本与组合试剂在常温下发生反应并显色,操作迅速简便,减少了加热对检测结果的干扰,检测结果直观性好;所用检测试剂种类较少,便于装配携带;可同时对多个油脂样本进行显色和检测,显色均匀,方便快捷,易于现场检测;所用检测试剂均低毒甚至无毒,安全环保。 The beneficial effect of the present invention is that: the present invention adopts the method of colorimetric analysis, the oil sample and the combined reagent react and develop color at normal temperature, the operation is quick and easy, the interference of heating to the detection result is reduced, and the detection result is intuitive; There are fewer types of detection reagents, which are easy to assemble and carry; it can develop and detect multiple oil samples at the same time, with uniform color development, convenient and fast, and easy on-site detection; the detection reagents used are all low-toxic or even non-toxic, safe and environmentally friendly. the
具体实施方式 Detailed ways
下面结合具体实施例对本发明进行进一步描述,但本发明的保护范围并不仅限于此: The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:
实施例1:本实施例的组合试剂为极性有机溶剂、还原剂、显色试剂、显色稳定剂;所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为3.6g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为528 g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为225 g/L。 Embodiment 1: the combination reagent of this embodiment is polar organic solvent, reducing agent, color developing reagent, color developing stabilizer; Described polar organic solvent is recorded by volume percentage, comprises 70% ethanol; Described reducing agent is by volume percentage Weight percent record, the mass concentration that comprises ferrous sulfate is 3.6g/L; Described color reagent is sulfosalicylic acid solution, and mass concentration is 528 g/L; Described color stabilizer is pyridoxamine hydrochloride solution , with a mass concentration of 225 g/L. the
首先,需要制作铁离子标准曲线图。所述铁离子标准曲线图的制作包括以下步骤: First, it is necessary to make a standard curve of iron ions. The making of described iron ion standard curve diagram comprises the following steps:
(a)将还原铁粉加入盐酸和过氧化氢溶解后,加热除去过氧化氢,冷却后用水稀释混匀,制得10mg/mL的铁标准储备液; (a) After adding the reduced iron powder into hydrochloric acid and hydrogen peroxide to dissolve, heat to remove the hydrogen peroxide, dilute and mix with water after cooling to obtain a 10mg/mL iron standard stock solution;
(b)利用步骤(a)制得的溶液以蒸馏水稀释的方法得到铁离子浓度为0、0.2、0.5、1、1.5、2.0、2.5、3.0、3.5、4.0 mg/mL的一系列梯度溶液,分别加入350μL的极性溶剂和10μL的显色稳定剂,震荡30秒; (b) Dilute the solution prepared in step (a) with distilled water to obtain a series of gradient solutions with iron ion concentrations of 0, 0.2, 0.5, 1, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0 mg/mL, Add 350 μL of polar solvent and 10 μL of color stabilizer, shake for 30 seconds;
(c)在步骤(b)得到的各溶液中加入还原剂150μL后震荡30秒反应3分钟,再加入显色试剂10μL; (c) Add 150 μL of reducing agent to each solution obtained in step (b), shake for 30 seconds and react for 3 minutes, then add 10 μL of color reagent;
(d)将步骤(c)得到的各溶液以10000r/min以上的转速离心10分钟,以铁离子浓度为0的反应管为参照在510nm处测定吸光度值; (d) Centrifuge each solution obtained in step (c) at a speed of 10,000 r/min or more for 10 minutes, and measure the absorbance value at 510 nm with reference to a reaction tube with an iron ion concentration of 0;
(e)分别以吸光度值、铁离子浓度为横、纵坐标绘制曲线,并用最小二乘法得到拟合的直线方程。 (e) Draw a curve with the absorbance value and iron ion concentration as the abscissa and ordinate, and use the least square method to obtain the fitted straight line equation. the
本实施例的采样油脂为小磨香油,一种食用油脂过氧化值测定方法,具体包括以下步骤: The sampling oil of the present embodiment is small ground sesame oil, a method for measuring the peroxide value of edible oils and fats, specifically comprising the following steps:
(1)用移液枪移取小磨香油20μL,记录下质量,加入极性有机溶剂350μL和显色稳定剂10μL的混合液,涡流震荡30秒; (1) Use a pipette gun to pipette 20 μL of small ground sesame oil, record the mass, add a mixture of 350 μL of polar organic solvent and 10 μL of color stabilizer, and vortex for 30 seconds;
(2)在步骤(1)的混合液中加入还原剂150μL,涡流震荡30秒,室温下静置3分钟; (2) Add 150 μL of reducing agent to the mixture in step (1), vortex for 30 seconds, and let stand at room temperature for 3 minutes;
(3)在步骤(2)的溶液中加入显色试剂10μL后,以10000r/min以上的转速离心旋转10分钟; (3) After adding 10 μL of chromogenic reagent to the solution in step (2), centrifuge at a speed of 10,000 r/min or more for 10 minutes;
(4)取步骤(3)得到混合溶液的上清部分在510nm处测定吸光度值; (4) Take the supernatant part of the mixed solution obtained in step (3) and measure the absorbance value at 510nm;
(5)根据吸光度值从铁离子标准曲线图上得到对应的铁离子浓度,并换算成单位质量的铁离子浓度作为过氧化值。 (5) Obtain the corresponding iron ion concentration from the iron ion standard curve according to the absorbance value, and convert it into the iron ion concentration per unit mass as the peroxide value. the
最后测定得到小磨香油的POV值为4.820mmol/Kg。 Finally, the POV value of the small milled sesame oil was determined to be 4.820mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.087mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of 0.087 mmol/L for the method. the
实施例2:本实施例中,所述极性有机溶剂按体积百分比记,包括30%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为20g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为528 g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为225 g/L。 Embodiment 2: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 30% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration comprising ferrous sulfate is 20g/L; The color reagent is a sulfosalicylic acid solution with a mass concentration of 528 g/L; the color stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 225 g/L. the
本实施例的采样油脂为玉米油,用移液枪移取玉米油20μL,其他实施方式与实施例1相同。 The sampling oil in this embodiment is corn oil, and 20 μL of corn oil is pipetted with a pipette gun, and other implementation methods are the same as in Example 1. the
最后测定得到玉米油的POV值为1.051mmol/Kg。 Finally, the POV value of the corn oil measured was 1.051mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.094mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of 0.094 mmol/L for the method. the
实施例3:本实施例中,所述极性有机溶剂按体积百分比记,包括90%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为1g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为528g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为225g/L。 Embodiment 3: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 90% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration including ferrous sulfate is 1g/L; The color reagent is a sulfosalicylic acid solution with a mass concentration of 528g/L; the color stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 225g/L. the
本实施例的采样油脂为菜籽油,用移液枪移取菜籽油20μL,其他实施方式与实施例1相同。 The sampling oil in this embodiment is rapeseed oil, and 20 μL of rapeseed oil is pipetted with a pipette gun, and other implementation methods are the same as in Example 1. the
最后测定得到菜籽油的POV值为1.119mmol/Kg。 Finally, the POV value of rapeseed oil measured was 1.119mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.121mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of 0.121 mmol/L for the method. the
实施例4:本实施例中,所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为1g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为1000g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为225g/L。 Embodiment 4: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 70% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration comprising ferrous sulfate is 1g/L; The color reagent is a sulfosalicylic acid solution with a mass concentration of 1000g/L; the color stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 225g/L. the
本实施例的采样油脂为大豆油,用移液枪移取大豆油20μL,其他实施方式与实施例1相同。 The sampling oil in this embodiment is soybean oil, and 20 μL of soybean oil is pipetted with a pipette gun, and other implementation methods are the same as in Example 1. the
最后测定得到大豆油的POV值为0.960mmol/Kg。 Finally, the POV value of the soybean oil measured was 0.960mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.134mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of the method of 0.134 mmol/L. the
实施例5:本实施例中,所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为20g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为300g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为225g/L。 Embodiment 5: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 70% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration comprising ferrous sulfate is 20g/L; The color reagent is a sulfosalicylic acid solution with a mass concentration of 300g/L; the color stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 225g/L. the
本实施例的采样油脂为花生油,用移液枪移取花生油20μL,其他实施方式与实施例1相同。 The sampling oil in this embodiment is peanut oil, and 20 μL of peanut oil is pipetted with a pipette gun, and other implementation methods are the same as in Example 1. the
最后测定得到花生油的POV值为1.910mmol/Kg。 Finally, the POV value obtained by measuring peanut oil was 1.910mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.100mmol/L。 The oil samples were serially diluted with ethanol to obtain a sensitivity of 0.100 mmol/L for the method. the
实施例6:本实施例中,所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为3.6g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为1000g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为200g/L。 Embodiment 6: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 70% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration including ferrous sulfate is 3.6g/L; The color developing reagent is a sulfosalicylic acid solution with a mass concentration of 1000g/L; the color developing stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 200g/L. the
本实施例的采样油脂为谷物调和油,用移液枪移取谷物调和油20μL,其他实施方式与实施例1相同。 The sampled oil in this embodiment is grain blended oil, and 20 μL of the grain blended oil is pipetted with a pipette gun, and other implementation methods are the same as in Example 1. the
最后测定得到谷物调和油的POV值为0.920mmol/Kg。 Finally, the POV value of the corn blended oil was determined to be 0.920mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.115mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of the method of 0.115 mmol/L. the
实施例7:本实施例中,所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为3.6g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为300g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为800g/L。 Embodiment 7: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 70% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration including ferrous sulfate is 3.6g/L; The color developing reagent is a sulfosalicylic acid solution with a mass concentration of 300g/L; the color developing stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 800g/L. the
本实施例的采样油脂为橄榄油,用移液枪移取橄榄油20μL,其他实施方式与实施例1相同。 The sampling oil in this embodiment is olive oil, and 20 μL of olive oil is pipetted with a pipette gun, and other implementation methods are the same as in Example 1. the
最后测定得到橄榄油的POV值为5.47mmol/Kg。 Finally, the POV value of the olive oil measured was 5.47mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.114mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of 0.114 mmol/L for the method. the
实施例8:本实施例中,所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为1g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为528g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为800g/L。 Embodiment 8: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 70% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration including ferrous sulfate is 1g/L; The color reagent is a sulfosalicylic acid solution with a mass concentration of 528g/L; the color stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 800g/L. the
本实施例的采样油脂为豆豉鲮鱼罐头中残留油,用移液枪移取豆豉鲮鱼罐头中残留油20μL,其他实施方式与实施例1相同。 The sampled oil in this example is the residual oil in canned dace with tempeh. Use a pipette gun to pipette 20 μL of the residual oil in canned dace with tempeh. Other implementation methods are the same as in Example 1. the
最后测定得到豆豉鲮鱼罐头中残留油的POV值为2.960mmol/Kg。 Finally, the POV value of the residual oil in the canned dace with tempeh was determined to be 2.960mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.099mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of the method of 0.099 mmol/L. the
实施例9:本实施例中,所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为3.6g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为528g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为225g/L。 Embodiment 9: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 70% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration including ferrous sulfate is 3.6g/L; The color developing reagent is a sulfosalicylic acid solution with a mass concentration of 528g/L; the color developing stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 225g/L. the
本实施例的采样油脂为花生油、菜籽油、橄榄油、小磨香油、谷物调和油、大豆油、玉米油按等比例混合的混合油,用移液枪移取混合油20μL,其他实施方式与实施例1相同。 The sampled oil in this example is peanut oil, rapeseed oil, olive oil, small ground sesame oil, grain blended oil, soybean oil, and corn oil mixed in equal proportions. Use a pipette to pipette 20 μL of the mixed oil. Other embodiments Same as Example 1. the
最后测定得到混合油的POV值为7.730mmol/Kg。 Finally, the measured POV value of the mixed oil was 7.730mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.087mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of 0.087 mmol/L for the method. the
实施例10:本实施例中,所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为3.6g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为528g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为225g/L。 Embodiment 10: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 70% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration including ferrous sulfate is 3.6g/L; The color developing reagent is a sulfosalicylic acid solution with a mass concentration of 528g/L; the color developing stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 225g/L. the
本实施例的采样油脂为鱼油,用移液枪移取鱼油20μL,其他实施方式与实施例1相同。 The sampled oil in this embodiment is fish oil, and 20 μL of fish oil is pipetted with a pipette gun, and other implementation methods are the same as in Example 1. the
最后测定得到鱼油的POV值为8.920mmol/Kg。 Finally, the POV value of the fish oil measured was 8.920mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.087mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of 0.087 mmol/L for the method. the
实施例11:本实施例中,所述极性有机溶剂按体积百分比记,包括70%的乙醇;所述还原剂按重量百分比记,包括硫酸亚铁的质量浓度为3.6g/L;所述显色试剂为磺基水杨酸溶液,质量浓度为528g/L;所述显色稳定剂为盐酸吡哆胺溶液,质量浓度为225g/L。 Embodiment 11: In the present embodiment, the polar organic solvent is recorded by volume percentage, including 70% ethanol; the reducing agent is recorded by weight percentage, and the mass concentration including ferrous sulfate is 3.6g/L; The color developing reagent is a sulfosalicylic acid solution with a mass concentration of 528g/L; the color developing stabilizer is a pyridoxamine hydrochloride solution with a mass concentration of 225g/L. the
本实施例的采样油脂为猪油,用移液枪移取猪油20μL,其他实施方式与实施例1相同。 The sampling fat in this embodiment is lard, and 20 μL of lard is pipetted with a pipette gun, and other implementation methods are the same as in Example 1. the
最后测定得到猪油的POV值为3.810mmol/Kg。 Finally, the POV value obtained by measuring lard is 3.810mmol/Kg. the
用乙醇逐级稀释油样,得到所述方法的灵敏度为0.087mmol/L。 The oil sample was serially diluted with ethanol to obtain a sensitivity of 0.087 mmol/L for the method. the
以上的所述乃是本发明的具体实施例及所运用的技术原理,若依本发明的构想所作的改变,其所产生的功能作用仍未超出说明书所涵盖的精神时,仍应属本发明的保护范围。 The above descriptions are specific embodiments of the present invention and the technical principles used. If changes are made according to the conception of the present invention, and the functions produced by it still do not exceed the spirit covered by the description, it should still belong to the present invention. scope of protection. the
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103776976A (en) * | 2014-01-17 | 2014-05-07 | 广东美味鲜调味食品有限公司 | Fast judging method for stability of peanut oil peroxide value |
CN104515770A (en) * | 2014-12-09 | 2015-04-15 | 苏州东辰林达检测技术有限公司 | Waste oil rapid detection kit and use method thereof |
CN106198419A (en) * | 2016-08-03 | 2016-12-07 | 哈尔滨普凡农牧有限公司 | Detect reagent and the method for α, β unsaturated aldehyde content in oil plant or oils and fats |
CN106383119A (en) * | 2016-11-08 | 2017-02-08 | 自贡勃生表面技术推广有限公司 | Spectrophotometry of rapidly detecting concentration of peroxide in edible oil |
CN107831126A (en) * | 2017-12-14 | 2018-03-23 | 广州傲农生物科技有限公司 | A kind of assay method of oil peroxidation value and its application |
CN109283161A (en) * | 2018-09-19 | 2019-01-29 | 青岛大学 | A method for detecting peroxide value in grease by using fluorescence quenching method |
CN110687108A (en) * | 2019-10-17 | 2020-01-14 | 山东瑞思康生物科技有限公司 | Multilayer film dry chemical reagent strip for quantitatively detecting urine malondialdehyde |
CN115894970A (en) * | 2022-12-23 | 2023-04-04 | 江南大学 | Color developing hydrogel for detecting freshness of vegetable oil and preparation method and application thereof |
WO2024130740A1 (en) * | 2022-12-23 | 2024-06-27 | 江南大学 | Color-developing hydrogel for testing freshness of vegetable oil, and preparation method therefor and use thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1540319A (en) * | 2003-04-27 | 2004-10-27 | 莫庆奎 | Method for testing peroxide value of liquid edible oil |
JP2005265811A (en) * | 2004-03-22 | 2005-09-29 | Oita Technology Licensing Organization Ltd | Method for measuring the amount of peroxide in cooking oil |
CN102706872A (en) * | 2012-06-13 | 2012-10-03 | 安徽省农业科学院农产品加工研究所 | Combined reagent for detecting peroxide value of edible oil and fat and detection method thereof |
-
2013
- 2013-05-07 CN CN201310166213.7A patent/CN103245660B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1540319A (en) * | 2003-04-27 | 2004-10-27 | 莫庆奎 | Method for testing peroxide value of liquid edible oil |
JP2005265811A (en) * | 2004-03-22 | 2005-09-29 | Oita Technology Licensing Organization Ltd | Method for measuring the amount of peroxide in cooking oil |
CN102706872A (en) * | 2012-06-13 | 2012-10-03 | 安徽省农业科学院农产品加工研究所 | Combined reagent for detecting peroxide value of edible oil and fat and detection method thereof |
Non-Patent Citations (2)
Title |
---|
于夕娟: "分光光度法测定食用油脂中过氧化值的研究", 《预防医学论坛》 * |
魏艳等: "磺基水杨酸显色分光光度法测定螯合剂螯合铁能力的研究", 《清洗世界》 * |
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CN103776976A (en) * | 2014-01-17 | 2014-05-07 | 广东美味鲜调味食品有限公司 | Fast judging method for stability of peanut oil peroxide value |
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CN106383119A (en) * | 2016-11-08 | 2017-02-08 | 自贡勃生表面技术推广有限公司 | Spectrophotometry of rapidly detecting concentration of peroxide in edible oil |
CN106383119B (en) * | 2016-11-08 | 2019-01-01 | 自贡勃生表面技术推广有限公司 | Quickly detect the photometry of peroxide concentrations in edible oil |
CN107831126A (en) * | 2017-12-14 | 2018-03-23 | 广州傲农生物科技有限公司 | A kind of assay method of oil peroxidation value and its application |
CN109283161A (en) * | 2018-09-19 | 2019-01-29 | 青岛大学 | A method for detecting peroxide value in grease by using fluorescence quenching method |
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CN115894970A (en) * | 2022-12-23 | 2023-04-04 | 江南大学 | Color developing hydrogel for detecting freshness of vegetable oil and preparation method and application thereof |
WO2024130740A1 (en) * | 2022-12-23 | 2024-06-27 | 江南大学 | Color-developing hydrogel for testing freshness of vegetable oil, and preparation method therefor and use thereof |
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