Summary of the invention
An object of the present invention is to provide N-acetylglucosamine or the acceptable salt of its dairy products as the application of additive in dairy products.
Another object of the present invention provides the dairy products that comprise N-acetylglucosamine or the acceptable salt of its dairy products.
Another purpose of the present invention provides a kind of method for preparing dairy products, comprises the step that adds N-acetylglucosamine or the acceptable salt of its dairy products in the raw milk goods.
Described N-acetylglucosamine full name is N-acetyl group-D-Glucosamine (N-acetyl-D-glucosamine), and molecular formula is C
8H
15NO
6, structural formula (I) is expressed as follows:
The example that can be used for N-acetyl group of the present invention-acceptable salt of D-Glucosamine dairy products includes but not limited to: with those salt of inorganic acid formation; example hydrochloric acid salt, phosphate, hydrophosphate; and those salt that form with organic acid, as citrate, benzoate, ascorbate.
To can be used for dairy starting material of the present invention and finished product dairy products of the present invention have virtually no restriction.Dairy products of the present invention can be any type of dairy products edible usually during people live, and for example, can be liquid as liquid milk; Solid-state as milk powder, cheese; Semisolid such as sour milk, ice cream etc.Described dairy products can be common dairy products, also can be that various fortification dairy products are as adding calcium milk etc.
Adding mode in dairy products is in fact also without any restriction, as long as described adding mode has no adverse effect to other active ingredient in N-acetyl group-D-Glucosamine or acceptable salt of its dairy products and the dairy products to N-acetyl group-D-Glucosamine or the acceptable salt of its dairy products.
In one embodiment of the invention, described N-acetyl group-D-Glucosamine or the acceptable salt of its dairy products can join in the milk powder with powder type.
In another embodiment of the invention; described N-acetyl group-D-Glucosamine or the acceptable salt of its dairy products can join in the liquid dairy product; these dairy products are sold like this then, or further adopt technology well known in the art to be prepared into for example milk powder, sour milk etc.
N-acetylglucosamine or the content of the acceptable salt of its dairy products in dairy products are generally 0.001g/100ml to 10g/100ml.Be preferably 0.01g/100ml to 2g/100ml.In the weight of the solid active principle in the dairy products, N-acetylglucosamine or the content of the acceptable salt of its dairy products in dairy products are generally 0.1-10%, preferred 0.1-2%.
The novel dairy product of the N-of comprising acetylglucosamine of the present invention or the acceptable salt of its dairy products has produced the new effect useful to body, and effect is remarkable in the crowd is on probation.
The specific embodiment
Further specify the present invention and beneficial effect thereof below in conjunction with embodiment, but should be understood that these embodiment only are in order to demonstrate the invention, and not in office where face constitutes limitation of the scope of the invention.
The short ripple test of embodiment 1. formulas (I) compound
1, test material and method:
1.1 sample: the pure product of formula (I) compound.
1.2 test material:
Bacterial classification: proteus mirabilis should meet following biochemical reaction feature: power (+), urease (+), lactose (-), glucose (+), H
2S (-), PD (+).
Culture medium: improvement LB culture medium (constituent: 1% tryptone, 0.5% yeast extract, 1% sodium chloride, 0.1% glucose, 0.002%TTC, PH7.2-7.4).
1.3 test method:
To in the same old way:, cultivated 9 hours for 37 ℃ the dull and stereotyped center dibbling proteus mirabilis of LB;
The test sample: the adding final concentration is 0.5% formula (I) compound in the LB flat board, with method dibbling proteus mirabilis, cultivates 9 hours for 37 ℃.
2, result of the test and evaluation:
In in the same old way, demonstrating constantly outwards expansion, at interval 3 hours concentric ring; In the test sample, not only form the concentric ring that occurred every 3 hours, and compared with the control, on every ring, demonstrate many tiny roll-collar.
Biological wave-wave dynamic test model is adopted in this test, short ripple effect in order to research formula (I) compound, visible (I) compound biological wave characteristic that bacterial cell is acted normally as a result, and make this fluctuation show finer fluctuation mode, show that fluctuation has facilitation to formula (I) compound to biology.This short ripple effect of formula (I) compound helps to explain that the N-acetylglucosamine promotes the effect that dairy products active ingredient absorbs.
The toxicological test of embodiment 2. formulas (I) compound
Carry out the toxicological test of formula (I) compound, comprising:
1, acute toxinology experiment: comprise oral, intravenous injection and greatest limit amount medicine-feeding test;
2, Salmonella reversion test;
3, mouse marrow cell micro nuclear test;
4, the abnormal property of mouse sperm test;
5, mouse testis chromosomal aberration test;
6, chronic lethal test;
7, subchronic toxicity (feeding in 90 days) test;
8, traditional teratogenicity test;
Conclusion (of pressure testing) shows: when formula (I) compound acute toxicity test dosage surpasses 2g/kg, do not occur the acute poisoning reaction yet; In long term toxicity test, maximum dose level has reached 1g/kg, and toxic reaction does not appear in experimental observation around the warp; In reproduction test, feed mouse from routine dose 7mg/kg, go down to posterity through three times, prove formula (I) compound to mouse become pregnant, gestation, childbirth, lactation and newborn mouse growth all do not have influence.These evidence formula (I) compounds belong to innocuous substance.
Embodiment 3. animal experiments
(1) increases appetite
Adopt Kunming mouse to test.Control group oral administration gavage milk, (addition: 0.1g/100ml milk), the result observes the test group mouse cycle of looking for food and shortens, and more foods are pulverized (it is active to represent animal to look for food) to add the N-acetylglucosamine in the test group milk, food-intake increases, and body weight obviously increases.
(2) promote suckling mouse to grow
Adopt Kunming mouse to test, get female mouse of pregnancy period earlier, be divided into test group and control group.Test group oral administration gavage dairy products of the present invention (adding N-acetylglucosamine 0.1g/100g milk powder in the commercially available dried milk powder), control group oral administration gavage commercially available dried milk powder, feed until suckling mouse birth lactation 15 days, two groups of sons of weighing are for the average weight of mouse always, and test group is apparently higher than control group.
Embodiment 4. preliminary crowd's proof tests
36 volunteer crowd's tests show, with drink general milk and compare, drink dairy products of the present invention (adding N-acetylglucosamine 0.1g/100ml in the sterilising fresh milk) person and reflect that appetite increases, the good appetite, when particularly gastrointestinal disease being arranged, effect is more obvious, it is fast to drink the conscious antisecosis in back, action is strong, and the state of mind is good, efficient reaching more than 90%.
The preparation embodiment of embodiment 5. dairy products of the present invention
1. with solid form the N-acetylglucosamine is joined in the milk, making N-acetylglucosamine content is the liquid milk of 0.1g/100ml.
2. with solid form N-acetylglucosamine hydrochloride is joined in the milk, making N-acetylglucosamine content is the liquid milk of 0.1g/100ml.
3. with solid form the N-acetylglucosamine is joined in the milk powder, making N-acetylglucosamine content is the blockmilk of 0.2g/100g.
4. with solid form the N-acetylglucosamine is joined in the sour milk after the fermentation, making N-acetylglucosamine content is the sour milk of 0.1g/100ml.
5. the form with 10% weightaqueous solution joins N-acetylglucosamine citrate in the milk, and making the final content of N-acetylglucosamine is the liquid milk of 0.1g/100ml.
Observation to above-mentioned dairy products is found, adds N-acetylglucosamine or its salt and the outward appearance of dairy products is not produced obviously influence.