CN1528169A - Method for extracting rice protein from rice - Google Patents
Method for extracting rice protein from rice Download PDFInfo
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- CN1528169A CN1528169A CNA031349730A CN03134973A CN1528169A CN 1528169 A CN1528169 A CN 1528169A CN A031349730 A CNA031349730 A CN A031349730A CN 03134973 A CN03134973 A CN 03134973A CN 1528169 A CN1528169 A CN 1528169A
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Abstract
The present invention discloses a method for extracting rice protein from rice. Said method includes the following steps: cleaning rice, soaking, removing sand, grinding to make pulp, concentrating and drying rice protein; also includes the steps of fine-grinding rice pulp, adopting dished separating machine to make multistage separation and making the protein pulp undergo the process of enzyme treatment. The described enzyme treatment is characterized by that in the multistage separated and combined rice protein pulp the following enzymes are successively added to make reaction: adding 0.05%-0.2% of amylase, temp. is 60-70 deg.C and time is 40-60 min; adding 0.05%-0.2% of cellulase, temp. is 40-50 deg.C and time is 2-3 hr; and adding 0.1%-1% of lipase, temp. is 40-60 deg.C and time is 2-3 hr. Said method can implement industrial production of rice protein.
Description
Technical field
The present invention relates to a kind of rice protein method of (comprise rice protein concentrate and rice separated protein, rice protein concentrate refers to that purity is the rice protein of 75%-89%, and rice separated protein refers to that purity is the rice protein more than 90%, and is together following) of from rice, extracting.
Background technology
Current research shows, compares with the soybean protein in animal protein and the vegetable protein, and rice protein has the hypoallergenic that other protein does not have, and is more suitable for especially consumption by infants of human body.In addition, rice protein has good nutritive value, and its amino acid ligand is more reasonable frequently, approaches adequate proteins, and biological value (BV) and protein score, PS (PV) be also than other vegetable protein height, is top grade in the vegetable protein.So rice protein is had very big demand both at home and abroad at present, China the extraction of rice protein as the country " 15 " Program for Tackling Key Problems.
Especially at present external bigger to the demand of rice separated protein, because the purity height of protein isolate, its fat and sugar content is very low, meets the requirement of the healthy food of current promotion.Few because of the rice separated protein impurities simultaneously, the also corresponding raising of absorption by human body rate.
Also have, residue behind the extraction albumen can be used for producing the commodity rice starch, highly purified rice starch becomes sour in storage hardly, is widely used in weaving, cosmetics, pharmacy, papermaking, food industry etc., is one of plant amylum of present most worthy.
The rice protein extracting method of at present studying morely both at home and abroad mainly contains alkaline process extraction, protease Enzymatic Extraction and the acid-hatching of young eggs and extracts.
(above background technology is referring to " progress of rice protein " grain and feed industry/2003.3 P44-45; " different protease extract the research of rice protein " grain and feed industry/2002.2 P41-42); " research of whole grain rice protein separation method " Heilungkiang science and technology of grain and oil/1998.4 P3-5.)
Alkaline extraction is to extract the most frequently used method of vegetable protein, but owing to protein content low (8%-12%) in the rice, need consume a large amount of alkali and water during extracting (extraction), and the protein concentration in the extract is very low, is difficult to be applied among the industrial production.
The extract content of protease extraction method and two kinds of methods of the acid-hatching of young eggs is all lower, and loss of proteins is more, and the gained purity of protein is not high, and the reaction time is long, and the reaction condition of enzyme is restive, is difficult to be applied to suitability for industrialized production.
On May 14th, 2003, publication number is " extracting the new technology of rice protein from rice is poor " patent application of CN1417344A, discloses the method for extracting rice protein with complex enzyme from rice is poor.It is raw material that this method adopts rice poor, needs to add the high temperature Ye Huamei but be pickled with grains or in wine from the rice to rice, and reaction temperature is up to 120-130 ℃, easily cause the serious sex change of rice protein, so that nutritive value reduces and influences absorption of human body, and simultaneously, this operation needs long period and more loaded down with trivial details technology.According to said method will obtain rice protein concentrate and rice separated protein, also handy alkaline process extracts, and that is to say to add a large amount of chemicals such as soda acid, and some country clearly limits and can not extract with chemicals the rice protein product; Chemicals such as while soda acid also have pollution to rice protein itself and environment; Adopt a large amount of soda acid of this arts demand consumption and water, also increased extraction cost, make operation become loaded down with trivial details simultaneously, extraction time is also corresponding longer.
Still use the relevant report that alkali extraction method can obtain the industrialized preparing process of rice protein concentrate and rice separated protein at present both at home and abroad invariably.
Summary of the invention
The technical problem to be solved in the present invention is, overcomes above the deficiencies in the prior art, and a kind of method of extracting rice protein from rice is provided.This method is an industrialized preparing process, can extract rice protein continuously, on a large scale, and it does not use alkali extraction method can obtain rice protein concentrate and rice separated protein.
Technical solution of the present invention is, this method comprises the processing step of following order: the cleaning of rice and immersion, desanding, defibrination, concentrated, dried rice albumen, it also comprises carries out fine grinding and adopts the disc separator multi-stage separation the Rice ﹠ peanut milk behind the defibrination, described fine grinding referred to above colloid mill twice, hole 0.01-0.1mm, time 10-15min.
Described multi-stage separation can be three grades of separation.
Described fine grinding is prepared for next step multistage video disc separation, and described multi-stage separation realizes farinaceous size to purify (gained starch is the dry rice starch that gets of technology routinely, down together), and the gained protein slurry merges.
Described dried rice albumen refers to the rice protein slurries concentrate drying that multi-stage separation merges is got rice protein concentrate (powder); Described drying is a pneumatic conveying drying, and baking temperature is below 80 ℃.
This method also can comprise to be handled the enzyme of protein slurry, promptly the enzyme that adds following percentage by weight in the rice protein slurries that multi-stage separation merges successively reacts, AMS enzyme concentration 0.05%-0.2%, reaction temperature 60-70 ℃, reaction time 40-60min; Cellulase enzyme concentration 0.05%-0.2%, reaction temperature 40-50 ℃, seasonable between 2-3 hour; Single fat enzyme enzyme concentration 0.1%-1%, reaction temperature 40-60 ℃, reaction time 2-3 hour.
It is as follows that described enzyme is handled optimal parameter: AMS enzyme concentration 0.1%, 65 ℃ of reaction temperatures, reaction time 40min; Cellulase enzyme concentration 0.1%, 45 ℃ of reaction temperatures, 2 hours reaction time; Single fat enzyme enzyme concentration 0.2%, 50 ℃ of reaction temperatures, 2 hours reaction time.
The effect that the above enzyme is handled is to slough remaining starch, cellulose and fat, finally obtains the higher rice separated protein of purity.
Described dried rice albumen refers to that herein the rice protein slurries concentrate drying after enzyme is handled gets rice separated protein (powder), and described drying is a pneumatic conveying drying, and baking temperature is below 80 ℃.
The present invention extracts the method for rice protein from rice, its concrete processing step (in proper order) is as follows:
(1) cleaning and the immersion of rice (preferably adopt early rice and crack rice): stirring and washing rice; Soak time: 2 hours summers, 3-4 hour spring and autumn, 4-6 hour winter.
(2) desanding: can adopt the eddy flow desanding, the parameter of hydrocyclone is 45 ° of cone angles, 0.1Mpa pressure, 25mm sand setting mouth.
(3) defibrination: particle sice in serosity is more than 80 orders, and concentration remains on 8%-15% (present specification percentage all refers to percentage by weight, below no longer explanation).
(4) fine grinding: cross twice above colloid mill, hole 0.01-0.1mm, time 10-15min.
(5) separate: through the disc separator multi-stage separation, the gained protein slurry merges.
(6) concentrate: separating obtained protein slurry is adopted plate compression.
(7) dried rice protein concentrate: will concentrate back rice protein slurries and adopt pneumatic conveying drying such as spray-drying or boiled bed drying can get rice protein concentrate (powder), baking temperature is below 80 ℃.
Obtain the higher rice separated protein of purity as need, then proceed following processing:
(8) the protein slurry enzyme is handled: promptly add following enzyme liquid successively in the rice protein slurries of gained and react after multi-stage separation: AMS enzyme concentration 0.05%-0.2%, reaction temperature 60-70 ℃, reaction time 40-60min; Cellulase enzyme concentration 0.05%-0.2%, reaction temperature 40-50 ℃, seasonable between 2-3 hour; Single fat enzyme enzyme concentration 0.1%-1%, reaction temperature 40-60 ℃, reaction time 2-3 hour.
(9) dried rice protein isolate: enzyme is handled rear slurry through concentrating, and adopts pneumatic conveying drying such as spray-drying or boiled bed drying, and baking temperature is below 80 ℃, gets rice separated protein (powder).
The physical and chemical index of the rice protein that employing this method is extracted is as follows:
Rice protein concentrate:
(1) protein content 〉=75% (butt)
(2) fat content≤4% (butt)
(3) ash content≤2% (butt)
(4) moisture≤10%
Rice separated protein:
(1) protein content 〉=90% (butt)
(2) fat content≤2% (butt)
(3) ash content≤1% (butt)
(4) moisture≤5%
Because adopt above production method, the present invention compares with the rice protein extracting method of prior art has following significant advantage and good effect:
(1) method of extracting rice protein is also only limited to theoretical research both at home and abroad at present, a kind ofly can extract the industrialized preparing process of rice protein continuously, on a large scale, especially from rice, extract the industrialized preparing process of rice protein concentrate and rice separated protein without alkaline process and the invention provides, all processes is realized mechanization, required labour is few, labour intensity is low, the production efficiency height.
(2) Chang Yong alkali extraction method easily causes the pollution of albumen itself and environment, the present invention take physical partition method get final product concentrated rice protein, adopt enzyme extraction method can get rice separated protein.These two kinds of products all do not contain chemicals, meet the notion of international " green health " advocated, satisfy the demand of some countries to this series products.
(3) this production technology reaction condition gentleness, drying mode is taked pneumatic conveying drying, and whole process of production maintains the temperature at below the rice protein denaturation temperature (80 ℃), and the albuminous degeneration degree is low, has farthest kept original character of rice protein.
(4) this production method is changed utilize solvent or the enzyme preparation separation method with the rice protein stripping over, take the mode of physical separation, starch in the rice and protein are separated, then can be again with enzyme with impurity in the protein (cellulose, starch, fat) stripping, therefore on each process procedure, all avoided the loss of protein, the recovery rate height.
(5) because this method adopts disc separator that albumen is separated with starch, protein slurry behind concentrate drying, get final product protein concentrate, its extraction time is short, technological operation is simple, extraction cost is low.
(6) this production method is less demanding to equipment, can all adopt home equipment, need not too many investment, is adapted to domestic manufacturer's suitability for industrialized production at present.
Description of drawings
Accompanying drawing extracts the process chart of the method for rice protein from rice for the present invention
The specific embodiment
Below in conjunction with the drawings and specific embodiments the present invention is made further describing, but the present invention is not limited only to following examples.
Embodiment one
Take by weighing early rice 200kg, drop into purge tank, clean the back and soaked 2 hours; Send into hydrocyclone, the hydrocyclone cone angle is 45 °, and pressure is 0.1Mpa, and the sand setting mouth is 25mm, the two-stage desanding; Drop into paste mill grinding to more than fineness 80 orders, concentration of slurry remains on about 10%; Slurries are imported colloid mill fine grinding twice, hole 0.1mm, 10 minutes time; Three grades of separation of slurries input disc separator (bottom, separation back grout outlet farinaceous size is sent in the ebullated bed dry after concentrating, and gets rice starch, and following examples are identical); Overfall protein slurry collection in top merges the back press filtration in three grades of separation, and filter residue is sent in the ebullated bed dry, and the control temperature of charge is lower than 80 ℃ and gets rice protein concentrate.The product of this embodiment meets quality standard, is qualified products, rice protein concentrate: purity is 78.9%, fat content 3.4%, and content of ashes is 1.88%, moisture content 4.5%.
Embodiment two
Take by weighing early rice 200kg, drop into purge tank, clean the back and soaked 2.5 hours; Send into hydrocyclone, the hydrocyclone cone angle is 45 °, and pressure is 0.1Mpa, and the sand setting mouth is 25mm, the two-stage desanding; Dropping into paste mill grinding to fineness is more than 80 orders, and concentration of slurry remains on about 10%; Slurries are imported colloid mill fine grinding twice, hole 0.08mm, time 12min; Three grades of separation of slurries input disc separator; Top overfall protein slurry is collected and is merged the back press filtration in three grades of separation of a part; Filter residue is sent in the ebullated bed dry, and the control temperature of charge is lower than 80 ℃ and gets rice protein concentrate.After top overfall protein slurry is collected and merged in three grades of separation of another part, enter in the enzymatic vessel, add following enzyme liquid successively and react: AMS: 20g enzyme concentration, 65 ℃ of reaction temperatures, PH=6.0,40 minutes reaction time; Cellulase: 20g enzyme concentration; 45 ℃ of reaction temperatures, PH=4.5,2 hours reaction time; Lipase: the 40g enzyme concentration, 50 ℃ of reaction temperatures, PH=7.0 reacted filter-press dehydration 2 hours; Filter residue is sent in the ebullated bed dry, and the control temperature of charge is lower than 80 ℃.The product of this embodiment meets quality standard, is qualified products, rice protein concentrate: purity is 80.7%, fat content 2.22%, and content of ashes is 1.52%, moisture content 8%; Rice separated protein: purity is 94.0%, fat content 1.90%, and content of ashes is 0.91%, moisture content 4.1%.
Embodiment three
Take by weighing early rice 200kg, drop into purge tank, clean the back and soaked 3 hours; Send into hydrocyclone, the hydrocyclone cone angle is 45 °, and pressure is 0.1Mpa, and the sand setting mouth is 25mm, the two-stage desanding; Drop into more than paste mill grinding to 80 order, concentration of slurry remains on about 10%; Slurries are imported colloid mill fine grinding twice, hole 0.05mm, time 15min; Three grades of separation of slurries input disc separator; After top overfall protein slurry is collected and merged in three grades of separation, enter in the enzymatic vessel, add following enzyme liquid successively and react: AMS: 30g enzyme concentration, 65 ℃ of reaction time, PH=6.0,1 hour reaction time; Cellulase: 30g enzyme concentration; 45 ℃ of reaction time, PH=4.5,3 hours reaction time; Lipase: the 50g enzyme concentration, in 50 ℃ of reaction time, PH=7.0 reacted filter-press dehydration 3 hours; Filter residue is sent in the ebullated bed dry, and the control temperature of charge is lower than 80 ℃.The product of this embodiment meets quality standard, is qualified products, rice separated protein: purity is 93.6%, fat content 2.0%, and content of ashes is 0.81%, moisture content 4.0%.
Claims (6)
1, a kind of method of from rice, extracting rice protein, it comprises the processing step of following order: the cleaning of rice and immersion, desanding, defibrination, concentrated, dried rice albumen, it is characterized in that: it also comprises carries out fine grinding and adopts the disc separator multi-stage separation the Rice ﹠ peanut milk behind the defibrination, described fine grinding referred to above colloid mill twice, hole 0.01-0.1mm, time 10-15min.
2, method according to claim 1 is characterized in that: described multi-stage separation refers to three grades of separation.
3, method according to claim 1 and 2 is characterized in that: described dried rice albumen refers to the rice protein slurries concentrate drying that multi-stage separation merges is got rice protein concentrate; Described drying is a pneumatic conveying drying, and baking temperature is below 80 ℃.
4, method according to claim 1 and 2, it is characterized in that: it also comprises handles the enzyme of protein slurry, promptly the enzyme that adds following percentage by weight in the rice protein slurries that multi-stage separation merges successively reacts: AMS enzyme concentration 0.05%-0.2%, reaction temperature 60-70 ℃, reaction time 40-60min; Cellulase enzyme concentration 0.05%-0.2%, reaction temperature 40-50 ℃, seasonable between 2-3 hour; Single fat enzyme enzyme concentration 0.1%-1%, reaction temperature 40-60 ℃, reaction time 2-3 hour.
5, method according to claim 4 is characterized in that: described AMS enzyme concentration 0.1%, 65 ℃ of reaction temperatures, reaction time 40min; Cellulase enzyme concentration 0.1%, 45 ℃ of reaction temperatures, 2 hours reaction time; Single fat enzyme enzyme concentration 0.2%, 50 ℃ of reaction temperatures, 2 hours reaction time.
6, method according to claim 5 is characterized in that: described dried rice albumen refers to that the rice protein slurries concentrate drying after enzyme is handled gets rice separated protein; Described drying is a pneumatic conveying drying, and baking temperature is below 80 ℃.
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100379352C (en) * | 2006-04-25 | 2008-04-09 | 曹镜明 | Rice protein ionization method |
| CN100399931C (en) * | 2006-04-25 | 2008-07-09 | 中南林业科技大学 | Microbial fermenting prepn. process for rice separated protein |
| CN100432108C (en) * | 2006-07-05 | 2008-11-12 | 江南大学 | Process for preparing rice starch and rice protein |
| CN100436592C (en) * | 2006-07-24 | 2008-11-26 | 华中农业大学 | Method for preparing water soluble starch |
| CN1896261B (en) * | 2006-06-15 | 2010-06-30 | 普洱永吉生物技术有限责任公司 | Production of rice-starch and rice-protein polypeptide powder by composite enzyme method |
| CN101869173A (en) * | 2010-07-06 | 2010-10-27 | 长沙高新开发区湘博医药科技有限公司 | Method for extracting rice protein powder by utilizing enzyme-alkali method |
| CN101695334B (en) * | 2009-09-09 | 2012-06-27 | 万福生科(湖南)农业开发股份有限公司 | Method for extracting rice protein by using rice |
| CN102876762A (en) * | 2012-09-25 | 2013-01-16 | 安徽省旌德县辉煌粮油有限责任公司 | Processing technology for extracting protein by broken rice enzyme method |
| CN101438785B (en) * | 2008-11-19 | 2013-01-23 | 莆田市东南香米业发展有限公司 | Technique for preparing edible rice protein and thin rice-flour noodles with coproduction of broken rice |
| CN103068264A (en) * | 2010-08-24 | 2013-04-24 | 丹尼斯科美国公司 | A food product comprising a low temperature rice protein concentrate |
| CN103250862A (en) * | 2013-04-01 | 2013-08-21 | 安徽禾粒源食品有限公司 | Sugar-free rice protein milk product and its production technology |
| CN103330047A (en) * | 2013-07-12 | 2013-10-02 | 普洱永吉生物技术有限责任公司 | Preparation method of rice protein |
| CN104814245A (en) * | 2015-04-03 | 2015-08-05 | 安徽天利粮油集团股份有限公司 | A complex extraction method for rice protein |
| CN110731409A (en) * | 2019-10-09 | 2020-01-31 | 安徽金太阳食品有限公司 | method for processing high protein rice flour with broken rice |
| CN111418702A (en) * | 2020-04-27 | 2020-07-17 | 安徽顺鑫盛源生物食品有限公司 | Production process of low-denaturation instant rice protein powder |
| CN112262915A (en) * | 2020-10-28 | 2021-01-26 | 江西恒顶食品有限公司 | Preparation method of instant rice protein powder |
| CN112790271A (en) * | 2020-12-24 | 2021-05-14 | 吉林中粮生化有限公司 | Method for extracting pea protein isolate and pea starch by micron method |
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| CN1210409C (en) * | 2002-12-05 | 2005-07-13 | 袁张度 | Technological process of extracting rice protein from distiller's grains |
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- 2003-09-30 CN CNB031349730A patent/CN1330251C/en not_active Expired - Fee Related
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100379352C (en) * | 2006-04-25 | 2008-04-09 | 曹镜明 | Rice protein ionization method |
| CN100399931C (en) * | 2006-04-25 | 2008-07-09 | 中南林业科技大学 | Microbial fermenting prepn. process for rice separated protein |
| CN1896261B (en) * | 2006-06-15 | 2010-06-30 | 普洱永吉生物技术有限责任公司 | Production of rice-starch and rice-protein polypeptide powder by composite enzyme method |
| CN100432108C (en) * | 2006-07-05 | 2008-11-12 | 江南大学 | Process for preparing rice starch and rice protein |
| CN100436592C (en) * | 2006-07-24 | 2008-11-26 | 华中农业大学 | Method for preparing water soluble starch |
| CN101438785B (en) * | 2008-11-19 | 2013-01-23 | 莆田市东南香米业发展有限公司 | Technique for preparing edible rice protein and thin rice-flour noodles with coproduction of broken rice |
| CN101695334B (en) * | 2009-09-09 | 2012-06-27 | 万福生科(湖南)农业开发股份有限公司 | Method for extracting rice protein by using rice |
| CN101869173A (en) * | 2010-07-06 | 2010-10-27 | 长沙高新开发区湘博医药科技有限公司 | Method for extracting rice protein powder by utilizing enzyme-alkali method |
| CN103068264A (en) * | 2010-08-24 | 2013-04-24 | 丹尼斯科美国公司 | A food product comprising a low temperature rice protein concentrate |
| CN102876762A (en) * | 2012-09-25 | 2013-01-16 | 安徽省旌德县辉煌粮油有限责任公司 | Processing technology for extracting protein by broken rice enzyme method |
| CN103250862A (en) * | 2013-04-01 | 2013-08-21 | 安徽禾粒源食品有限公司 | Sugar-free rice protein milk product and its production technology |
| CN103330047A (en) * | 2013-07-12 | 2013-10-02 | 普洱永吉生物技术有限责任公司 | Preparation method of rice protein |
| CN103330047B (en) * | 2013-07-12 | 2015-04-22 | 普洱永吉生物技术有限责任公司 | Preparation method of rice protein |
| CN104814245A (en) * | 2015-04-03 | 2015-08-05 | 安徽天利粮油集团股份有限公司 | A complex extraction method for rice protein |
| CN110731409A (en) * | 2019-10-09 | 2020-01-31 | 安徽金太阳食品有限公司 | method for processing high protein rice flour with broken rice |
| CN111418702A (en) * | 2020-04-27 | 2020-07-17 | 安徽顺鑫盛源生物食品有限公司 | Production process of low-denaturation instant rice protein powder |
| CN112262915A (en) * | 2020-10-28 | 2021-01-26 | 江西恒顶食品有限公司 | Preparation method of instant rice protein powder |
| CN112790271A (en) * | 2020-12-24 | 2021-05-14 | 吉林中粮生化有限公司 | Method for extracting pea protein isolate and pea starch by micron method |
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