CN100436592C - Method for preparing water soluble starch - Google Patents
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- CN100436592C CN100436592C CNB2006100196830A CN200610019683A CN100436592C CN 100436592 C CN100436592 C CN 100436592C CN B2006100196830 A CNB2006100196830 A CN B2006100196830A CN 200610019683 A CN200610019683 A CN 200610019683A CN 100436592 C CN100436592 C CN 100436592C
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Abstract
The process of preparing water soluble starch includes the following steps: washing grain or crop tuber material, soaking in alkali solution and making starch slurry; neutralizing, water washing to desalt and centrifuging to collecting wet starch; drying and crushing to obtain crude starch; and grinding crude starch in ball mill to obtain water soluble starch. The preparation process features the increased fermentation step between the material washing step and the alkali solution soaking step and the increased enzymolysis step between water washing and desalting step and the centrifuging step. In the fermentation step, artificially inoculated fermenting microbe or natural microbe is utilized; and in the enzymolysis step, proteinase is utilized. The water soluble starch product has fast dissolving and high solubility over 90 %.
Description
Technical field
The invention belongs to the starch processing technique field, specifically, the present invention relates to a kind of preparation method of water soluble starch of ultrafine powder.
Background technology
China's starch resource is abundant, particularly cereal and potato starch resource, and output all accounts for the No. 1 in the world.The deep processing of research cereal and potato class is significant to improving added value.
Along with the development of foodstuffs industry and material industry, water soluble starch more and more is subject to people's attention.Cool water soluble starch mainly contains pre-gelatinized starch and granular cold water soluble starch at present.The preparation method of pre-gelatinized starch adds water furnishing starch milk with ative starch, heat pre-gelatinization after, use roller drying, spraying drying or extrusion expansion again, the starch of preparation is called pre-gelatinized starch.Pre-gelatinized starch is the shred of non-particulate type, and the paste difference that low in glossiness, state of sticking with paste after the rehydration and character and ative starch are made is very big, and its application is subjected to certain restriction.
At present, the investigator generally adopts double flowing nozzle spray-drying process (Pitchon etc. both at home and abroad, Process for Cooking orGelatinizing Materials.U S Patent:4280851,1981), High Temperature High Pressure alcohol method (Eastman J E etc., Cold WaterSoluble Granular Starch for Gelled food Composition.U S Patent:4465702,1984.), normal pressure polyvalent alcohol method (Kurakake M etc., Effects on Maize Starch Properties of Heat-treatment with Water-EthanolMixtures.J Cereal Sci, 1997 (25): 253-260.) with pure alkali combination treatment method (Jane J etc., Characterization ofGranular Cold-Water-Soluble Starch.Starch, 1986 (38:258-263.1997; Cory M etc., Effect of Lime onGelatinization of Corn Flour and Starch.Cereal Chem, 1997 (74): 171-175; Ou Shiyi etc., cold water water soluble starch new preparation process, food and machinery, 1997,6:24; For Yulin etc., the development of granular cold water soluble starch, guizhou chemical industry, 2004,29 (6): 15-16) etc. method prepares cool water soluble starch, and the cool water soluble starch of these methods preparations is particulate state, and character is similar to the starch after the boiling.Though the water soluble starch purposes of these method preparations is wide, these preparation methods need consume a large amount of alcohol or alkali, contain compositions such as pure and mild alkali in the product, and purity is relatively poor, and the wastewater treatment difficulty is big.The intermediate product that generates in the alcohol that contains in the product, alkali and the treating processes also may cause food-safety problem.
There is data to show, adopts the mechanical ball milling method to grind more than the 50h, can obtain at normal temperatures just starch (Hu Fei etc., the physico-chemical property of miniaturization yam starch, Wuxi Light Industry Univ.'s journal, 2002,21 (5): 452-455) of energy gelatinization.Can prepare granular cold water soluble starch (Wang Yang etc. by ball milled, the new method preparation and the structure preliminary study thereof of granular cold water soluble starch, casting, 2005,11 (54): 1069-1072), can obtain 30 ℃ of solubleness when grinding 40h and be 70% product.But the water soluble starch of Prepared by Ball Milling exists the shortcomings such as starch dissolution poor performance of length consuming time, preparation.
Summary of the invention
The object of the invention is to overcome the defective of prior art, and a kind of method for preparing the water soluble starch of high resolution is provided, to reach the purpose of easy, safety, environmental protection.
The present invention realizes by following technical proposal:
A kind of preparation method of water soluble starch, its step comprises: the raw material that will contain starch is cleaned, dipping by lye, the slurrying of band water obtains starch slurry; This starch slurry of neutralizing treatment, washing desalination, centrifugal collection wet starch; To obtain ative starch after the described wet starch drying and crushing, again this ative starch is carried out ball milling and obtain water soluble starch, main points of the present invention are, described raw material clean with the dipping by lye step between fermentation step of increase; Between washing desalination and centrifugal collection step, increase an enzymolysis step; Described fermentation step comprises: will inoculate bio-fermentation agent (for example milk-acid bacteria) in the described material or utilize the natural microorganisms in environment or the material to ferment; Described enzymolysis adopts proteolytic enzyme; Described grinding steps adopts ball milling method, and the final thickness that makes the finished product starch granules is less than 300nm.
Fermentation step of the present invention is as follows: add the water of 1-2 times of weight by weight in the raw material after cleaning, (described starter is by 10 in inoculation in accordance with the method for claim 1
6-10
12The dried raw material inoculum size inoculation of cfu/100g), described material is under anaerobic fermented, the control leavening temperature is 30-45 ℃, and fermentation time is 15-35h.
Enzymolysis step of the present invention is as follows: the pH that neutralizing treatment is washed the starch slurry after the desalination transfers to about 8.5; In starch: the ratio of water (W/W) transfers to 8%-20% with starch slurry concentration; Again enzyme is lived for 18-30 ten thousand U/ml Sumizyme MPs in Sumizyme MP: the envelope-bulk to weight ratio of dried raw material is to be hydrolyzed in the starch slurry after the ratio of the dried raw material of 20 μ l/100g dried raw material-400 μ l/100g is added described desalination to, hydrolysis temperature is 30-60 ℃, hydrolysis time 1-8h.
Ball milling step of the present invention is, the water content of the ative starch of preparation or the starch that is purchased is transferred to 4%-18%, and the weight ratio by abrading-ball and starch is 1 again: 1-3.3: 1 abrading-ball and starch are put into the mill jar of ball mill, control its rotating speed and are not less than 200r/min.
In one of them embodiment of the present invention, described abrading-ball is that the abrading-ball of 1cm and the weight ratio of the abrading-ball that diameter is 0.6cm are 3.2 by diameter: 0-0: 1 configuration.
In order to implement the present invention better, described ball mill is pressed forward-order (for example each hour rotated the sense of rotation of primary ball mill) reverse and reverse-forward and is moved, with the milling time of shortening material.
Basic preparation method's of the present invention technical process as shown in Figure 1.
Concrete technology is such:
Take by weighing a certain amount of raw material, add tap water and clean three times (being purchased starch can omit from the process of feedstock production starch), add tap water, making material-water ratio is 3: 4 (by weight), transfer pH6-9 under 30-45 ℃ of condition, to inoculate (for example milk-acid bacteria) or utilize the microorganism in material itself or the environment to carry out spontaneous fermentation, wherein inoculation fermentation adopts general milk-acid bacteria (Deng Kaiye etc., the different fermentations agent is to the influence of fermented rich beverage quality. grain and oil processing and food machinery, 2006 (3): 82-84,2006; Wang He etc., the screening of rice lactic fermenting beverage bacterial strain, Heilongjiang Commerce College CN)'s journal (natural science edition), 2000,16 (1): 16-20), press the dried raw material inoculum size of 106-1012cfu/100g inoculating lactic acid bacterium; Behind the fermentation 15-35h, abandoning supernatant adds 0.4% NaOH solution soaking 24 hours (weight of NaOH solution is 3 times of raw material weight).Add new 0.4% NaOH solution defibrination then, make the weight of final NaOH solution be about 6 times of raw material weight, soak 24h again, every 6h renews NaOH once, with the neutralization of HCl solution, washes 5 times then, transfer starch slurry concentration be 8%-20% (press starch: water, w/w).After transferring about pH to 8.5, add Sumizyme MP (the Alcalase enzyme that letter Tianjin company of Novi provides is a Sumizyme MP), the consumption of this enzyme (enzyme is lived and is 18-30 ten thousand U/ml) is that the dried raw material of 20 μ l/100g dried raw material-400 μ l/100g adds, in 30 ℃ of-60 ℃ of following hydrolysis 1-8h, add tap water then and clean, sedimentation 12h removes supernatant liquor, remaining starch slurry takes off a layer starch with the centrifugal 10min of 2000r/min, and drying obtains ative starch.The water content of starch transferred to be 4%-18%, get the starch of certainweight, by abrading-ball: the weight ratio of material is 1: 1-3.3: 1 adds abrading-ball, and (abrading-ball is that the abrading-ball of 1cm and the weight ratio of the abrading-ball that diameter is 0.6cm are 3.2 by diameter: 0-0: 1 configuration, the cumulative volume of material and abrading-ball be no more than spherical tank volume 1/2), put into jar.The speed setting of ball mill is 200-800r/min, and its sense of rotation is set at the sense of rotation of per hour changing primary ball mill according to forward-reverse-forward-reverse sequence, to improve the preparation efficiency of water soluble starch.
Comparing the present invention with existing water soluble starch technology of preparing has the following advantages:
1, prepare water soluble starch with chemical process and compare, the present invention adopts ball milling method to prepare water soluble starch, obtains sheet, starch that particle is tiny.This method has purity and yield height, and instantly-soluble is good, characteristics such as applied range.
2, the general uncomfortable water saving branch of Prepared by Ball Milling water soluble starch, the present invention adjusts the water content of ative starch before ball milling, make starch become crisp, helps improving dissolution rate, reduces milling time.
3, compare with general ball milled, the present invention adds fermentation and two operations of enzymolysis in the preparation process of ative starch, can improve purity of starch and yield on the one hand, can effectively improve the starch granules structure on the other hand, reduce hardness, help the fragmentation of starch, thereby shorten milling time, improve the solvability of starch.
4, general ball milled is medium with ethanol, and the present invention does not add medium when grinding, and the water soluble starch security that obtains is good, pollution-free, and helps the dispersion and the dissolving of starch.
5, the water soluble starch of general Prepared by Ball Milling is a particulate state, though pre-gelatinized starch is a sheet, starch molecule is swelling fully, and particle size is bigger, exists dissolution rate to wait shortcoming slowly.The water soluble starch particle that the present invention obtains is tiny, is sheet, and lamellar spacing is less than 300nm, thereby makes the enhanced dissolution rate of starch, dissolution rate height (dissolution rate under 25 ℃ is greater than 90%).
6, the prior art water soluble starch of doing medium preparation with ethanol exists coldwater-soluble poor, defective such as lump among the Yi Zaishui, the water soluble starch that the present invention obtains, coldwater-soluble is good, water soluble starch is put in to stir in 25 ℃ of water get final product dispersing and dissolving or form transparent colloid.
Description of drawings
Fig. 1 is a techniqueflow chart of the present invention.
Fig. 2 be in one embodiment of the present of invention the ative starch water content to the influence of water soluble starch dissolution rate under the ball milling condition;
Condition among Fig. 2 is: the ative starch water content is 0.62-10.45% before the ball milling, and the weight ratio of abrading-ball and starchy material is 3.13, and drum's speed of rotation is for setting 480r/min.
Fig. 3 is that milling time influences the water soluble starch dissolution rate in one embodiment of the present of invention;
Condition among Fig. 3 is: the ative starch water content is 5.10% before the ball milling, and the weight ratio of abrading-ball and starchy material is 3.13, and drum's speed of rotation is set at 480r/min.
Fig. 4 is the influence of the rotating speed of ball mill in one embodiment of the present of invention to the water soluble starch dissolution rate;
The condition of Fig. 4 is: the water content of ative starch is 5.10% before grinding.
Fig. 5 is the comparison of the Different Weight comparison water soluble starch dissolution rate of abrading-ball and starchy material in one embodiment of the present of invention.
The condition of Fig. 5 is: the ative starch water content is 5.10% before grinding.
Fig. 6 is the scanning electron microscope picture of the water soluble starch of ative starch and the present invention preparation in one embodiment of the present of invention.
Fig. 7 is the comparison of different milling time water soluble starch dissolution rates under fermentation or the nonfermented condition in one embodiment of the present of invention;
The condition of Fig. 7 is: the ative starch water content is 5.10% before grinding, and the weight ratio of abrading-ball and starchy material is 3.13, and drum's speed of rotation is 480r/min.
Embodiment
Embodiment 1 preparation method for example
Material: experiment material adopts commercially available long-grained nonglutinous rice, and chemical reagent is analytical pure.From commercial acquisition.
1. the extraction process of ative starch
(1) clean: take by weighing 3kg raw material (long-grained nonglutinous rice) and put into the 10L plastic tank, add tap water and clean raw material 3 times, add tap water, making material-water ratio is 3: 4;
(2) soak: added concentration and be 0.4% NaOH solution soaking long-grained nonglutinous rice 24 hours, the weight of NaOH solution is 3 times of raw material weight;
(3) slurrying: the raw band soak solution after will soaking is defibrination together, and the NaOH solution of defibrination limit, limit interpolation 0.4%, and the weight that makes alkaline solution is 6 times (starch material omissions) of raw material weight;
(4) neutralization and washing: continue to soak 24h, every 6h renews alkali lye once, among the HCl and after washing 5 times;
(5) enzyme is handled: transferring starch slurry concentration is 20%, adds Sumizyme MP, and consumption is the dried raw materials of 100 μ l/100g, 50 ℃ of following hydrolysis 2h;
(6) sedimentation: add tap water and clean sedimentation 12h;
(7) centrifugal: remove supernatant liquor, remaining starch slurry takes off a layer starch with the centrifugal 10min of 2000r/min;
(8) drying: natural air drying obtains ative starch.
2. ball-milling technology condition
Ball milling method is as follows:
(1) ative starch is put into 45 ℃ baking oven water content is adjusted to 5.00%-10.24%;
(2) take by weighing ative starch 30-50g and put into agate jar;
(3) take by weighing agate ball milling pearl (wherein the ball weight ratio of diameter 1cm and diameter 0.6cm is 3.2: 1) 94-74g, put into jar;
(4) the agate jar is put into the Ma Taozhong that draws of ball mill, added a cover setup parameter.Its ball mill parameter is: per hour change the sense of rotation of forward-oppositely and oppositely-forward, rotating speed 400-500r/min.
Milling parameters:
With ball material weight ratio, drum's speed of rotation and milling time is that factor is carried out single factor and orthogonal test.Dissolution rate with starch after grinding is that index is determined the ball-milling technology condition.
The measuring method of starch dissolution rate is as follows: get 1.0g wet basis starch in the 50ml centrifuge tube, add water 25g, shake up.In 25 ℃ of following stirring in water bath 30min, take out immediately with the centrifugal 20min of the speed of 3000r/min, carefully pour out supernatant liquor, the supernatant liquor total mass is Vg, gets the 10g supernatant liquor, places known weight (W
2) glassware in, weigh in drying by the fire to complete drying under 105 ℃, be designated as W
1(g).The calculation formula of dissolution rate is:
1. the ative starch water content is to the influence of starch dissolution rate
The preparation of the ative starch of different moisture content: it is dry that the Starch rice after air-dry is put into 45 ℃ of baking ovens, every two hours get sample one time, obtaining water content is the rice ative starch of 10.45%-5.10%, by after with water content be 5.10% starch put into 105 ℃ dry, every 30min gets sample one time, and further obtaining water content is the rice ative starch of 0.62%-3.14%.
Fig. 2 has shown that ball material weight ratio is 3.13: 1, and rotating speed is 480r/min, and the ative starch water content was to the influence of starch dissolution rate when milling time was 20h.As shown in Figure 2, when the ative starch water content was between 3.14%-6.16%, the dissolution rate of starch was higher, because starch exsiccant equilibrium water content in 45 ℃ is about 5.10%, therefore selected for use 5.10% water content more suitable.
2. milling time is to the influence of starch dissolution rate
Fig. 3 has shown that the ative starch water content is 5.10%, and ball material weight ratio is 3.13: 1, and milling time was to the influence of ative starch dissolution rate when rotating speed was 480r/min.As shown in Figure 3, when milling time is 80h when above, the dissolution rate of starch changes and tends towards stability.
3. rotating speed is to the influence of starch dissolution rate
Fig. 4 has shown that the ative starch water content is 5.10%, and when grinding 80h, rotating speed is to the influence of starch dissolution rate under the different ball material weight ratios.Its medium speed can reflect the size of abrading-ball to starch storage crop power, and ball material weight ratio then can improve the size-grade distribution of broken speed and starch.As shown in Figure 4, ball material weight ratio is big more or rotating speed is high more, and the dissolution rate of starch is high more.And ball material weight ratio is 3.13 o'clock, and dissolution rate is bigger between 400r/min and 450r/min.Along with the increase of rotating speed, abrading-ball increases the squeeze and the shearing force of starch, and the increase of effect frequency, thereby the crushing effect of starch is strengthened, and finally causes the dissolution rate of starch to increase.But when frequency was increased to certain value, gathering way of dissolution rate slowed down.
4. ball material weight ratio is to the influence of starch dissolution rate
Fig. 5 has shown that the ative starch water content is 5.10%, and when grinding 80h, ball material weight ratio is to the influence of starch dissolution rate under the different rotating speeds.As shown in Figure 5, ball mill is than (300r/min and 350r/min) under the slow speed of revolution, and the dissolution rate of starch is less, and presents ascendant trend always with the variation of ball material weight ratio; Under higher rotation speed (400r/min-500r/min), the dissolution rate of starch is bigger, and after ball material weight ratio was greater than 2.1, it is slow that the growth of dissolution rate becomes.
In sum, the suitable milling parameters of water soluble starch is 4%-6%, ball material weight ratio 2.54-3.13, rotating speed 420-480r/min for the ative starch water content, grinds more than the 80h.
The analysis of physical and chemical property of embodiment 2 water soluble starchs
Test materials is commercially available long-grained nonglutinous rice rice, and it is pure that each reagent is commercially available chemical analysis.The QM-1SP2 type planetary ball mill that ball mill adopts Nanjing Univ. Instrument Factory to produce, ball grinder and abrading-ball are the agate quality.
The ative starch preparation method who implements in 1 is adopted in the preparation of rice ative starch, the rice ative starch (water content is 13%) of preparation is put in dry 12h in 45 ℃ of baking ovens, water content is 5.10%, get 30g starch and be put in the ball grinder, adding particle diameter is the agate ball 71.6g of 1cm, the agate ball 22.4g of 0.6cm, insert in the planetary ball mill, the setting rotational speed of ball-mill is 480r/min, grinds 0-300h, measures dissolution rate, reducing sugar content, transparency and the kinematic viscosity of starch.
Dissolution rate is seen the method among the embodiment 1;
The mensuration of reducing sugar content adopts the DNS method, specific as follows: sample thief (ative starch) 0.5g is in the 50ml colorimetric cylinder, adding less water earlier shakes up, the back uses the distilled water constant volume to 50ml, in boiling water bath, be incubated 30min, the centrifugal 10min of 3000r/min, draw the 1ml supernatant liquor in test tube, (compound method is with reference to Zhang Weijie (Zhang Weijie to add 0.5mlDNS reagent, saccharide complex Biochemical Research technology, Hangzhou, press of Zhejiang University, 1994) method) mixing is (with 1ml distilled water, 0.5mlDNS the blank of work) behind the boiling water bath insulation 5min, add 3.5ml distilled water in 540nm place colorimetric, survey light absorption value A, according to typical curve light absorption value is converted into reducing sugar content (%) then with the 1cm colorimetric cylinder.
The mensuration of transparency adopts colorimetry, specific as follows: as to get 0.5g starch (dry weight), the colorimetric cylinder of putting into 50ml is made into 1% starch milk, stirring heating 30min in boiling water bath, be cooled to room temperature, vibration is evenly done blank with distilled water, measure transmittance with spectrophotometer down in the 620nm wavelength, be transmittance.
Kinematic viscosity adopts State Standard of the People's Republic of China GB5516-85, and adopting the concentration of starch milk is 0.5g starch/20ml water.
Milling time sees Table 1 to the influence of starch property.As can be seen from Table 1, prolongation along with milling time, the dissolution rate of starch, reducing sugar content and transparency are all in rising trend, the dissolution rate of ative starch, reducing sugar content and transparency are respectively 0.47%, 0.55% and 9.11%, and dissolution rate, reducing sugar content and the transparency of starch are respectively 95.53%, 12.29% and 90.33% behind the grinding 200h.Hence one can see that, and dissolution rate, reducing sugar content and the transparency of grinding starch have bigger influence.
Table 1 milling time is to the influence of starch property
Adopt scanning electronic microscope that the pattern of starch granules is analyzed, the scanning electron microscope picture that obtains ative starch and water soluble starch is seen Fig. 6.As shown in Figure 6, the particle of former Starch rice is a polyhedral structure, and the surface is more smooth, and median size is about 4 μ m.The water-soluble rice starch that grinds 100h is laminar, and shape is not of uniform size, and lamellar spacing is less than 300nm.
The different starchy materials of embodiment 3 usefulness prepare water soluble starch for example
The corn ative starch adopts marketable material (Henkel Food Co., Ltd provides by Wuhan, Chinese Wuhan City, Hubei Province), Starch rice adopts the alkaline leaching preparation shown in the embodiment 1, spontaneous fermentation (35 ℃, 30h), add proteolytic enzyme (the Alcalase enzyme that letter Tianjin company of Novi provides is a Sumizyme MP) and handle (adding the Sumizyme MP of the dried raw material of 100 μ l/100g, reaction 30h).Wheat starch adopts WATER-WASHING METHOD (Yao Danian etc., the relation of starch physicochemical property, genetic development and wheat starch and quality, grain and fodder industry, 1997,2:36-38) preparation, sweet potato starch adopts conventional centrifugal settling method (Fan Lisheng, the research of sweet potato starch fundamental characteristics, grain and fodder industry, 2001,2:49-51) preparation.
Each starch 30g is put in the ball grinder, and adding particle diameter is the agate ball 71.6g of 1cm, the agate ball 22.4g of 0.6cm, insert in the planetary ball mill, the setting rotational speed of ball-mill is 480r/min, grinds 150h, measures the dissolution rate and the reducing sugar content of ative starch and water soluble starch.Measurement result sees Table 2.As shown in Table 2, be that material adopts method of the present invention all can make water soluble starch with rice, corn, wheat and sweet potato, but bigger with the dissolution rate of rice and sweet potato starch.
Table 2 is used the influence of technology of the present invention to rice, corn, wheat and sweet potato leading indicator
The comparison of different milling time water soluble starch dissolution rates under embodiment 4 fermentations or the nonfermented condition
Soaking fermentation step (plastic tank is put into 35 ℃ incubator spontaneous fermentation 30h, abandoning supernatant) is added in preparation process (1) and (2) at embodiment 1 rice ative starch, and other steps and method are with embodiment 1.Compare with the water soluble starch dissolution rate that does not add the fermentation step preparation adding fermentation step, the results are shown in shown in Figure 7.As shown in Figure 7, add fermentation step and can effectively improve dissolution rate, when grinding 50h, the dissolution rate that adds fermentation step is higher by 23% than the dissolution rate that does not add fermentation step.
Terminological interpretation:
1, dried raw material: the alleged dried raw material of claims of the present invention and specification sheets is meant the raw material that does not add water or carry out any processing;
2, material: the alleged material of claims of the present invention and specification sheets is meant the raw material that obtains after water or at least one processing through adding.
Claims (4)
1, a kind of preparation method of water soluble starch, its step comprises: the raw material that will contain starch is cleaned, dipping by lye, the slurrying of band water obtains starch slurry; This starch slurry of neutralizing treatment, washing desalination, centrifugal collection wet starch; To obtain ative starch after the described wet starch drying and crushing, this ative starch is ground obtain water soluble starch again, it is characterized in that: described raw material clean with the dipping by lye step between fermentation step of increase; Between washing desalination and centrifugal collection step, increase an enzymolysis step; Described fermentation step comprises: the water that adds 1-2 times of weight by weight in the raw material after cleaning, in described material inoculating lactic acid bacterium or utilize environment or material in natural microorganisms ferment, described material is under anaerobic fermented, the control leavening temperature is 30-45 ℃, fermentation time is 15-35h, and described enzymolysis adopts proteolytic enzyme; Described grinding steps adopts ball milling method, makes finished product starch be laminar, and lamellar spacing is less than 300nm.
2, the preparation method of water soluble starch according to claim 1 is characterized in that, described milk-acid bacteria is by 10
6-10
12The dried raw material inoculum size inoculation of cfu/100g.
3, the preparation method of water soluble starch according to claim 1 is characterized in that: described enzymolysis step is as follows: the pH that neutralizing treatment is washed the starch slurry after the desalination transfers to 6-9; In starch: the ratio of water (W/W) transfers to 8%-20% with starch slurry concentration; Again enzyme is lived for 18-30 ten thousand U/ml Sumizyme MPs in Sumizyme MP: the envelope-bulk to weight ratio of dried raw material is to be hydrolyzed in the starch slurry after the ratio of the dried raw material of 20 μ l/100g dried raw material-400 μ l/100g is added described desalination to, hydrolysis temperature is 30-60 ℃, hydrolysis time 1-8h.
4, according to the preparation method of each described water soluble starch of claim 1-3, it is characterized in that, described ball milling step is, the water content of the ative starch of described preparation is transferred to 4-18%, weight ratio by abrading-ball and starch is 1 again: 1-3.3: 1 abrading-ball and starch are put into the mill jar of ball mill, and controlling its rotating speed is 200r/min-480r/min.
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| WO2010129648A2 (en) * | 2009-05-07 | 2010-11-11 | Danisco Us Inc. | Starch separation process |
| CN102161705A (en) * | 2011-05-27 | 2011-08-24 | 沁源县博翔新能源科技发展有限公司 | Method for extracting starch from crop straws |
| CN110903405B (en) * | 2019-12-03 | 2022-04-12 | 安徽山河药用辅料股份有限公司 | Preparation method of completely pregelatinized starch |
| CN111330728B (en) * | 2020-04-29 | 2021-06-08 | 广西大学 | Method for separating starch with different particle sizes by polyethylene glycol 200 solution gravity suspension |
| CN113229485A (en) * | 2021-05-19 | 2021-08-10 | 南京泛成生物科技有限公司 | Preparation method and application of instant plant starch |
| CN115160446A (en) * | 2022-08-19 | 2022-10-11 | 宁波工程学院 | Preparation method of nano starch |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448067A (en) * | 2003-04-28 | 2003-10-15 | 江南大学 | Process of preparing glutathione-enriched wheat-germ water soluble extractive |
| CN1528169A (en) * | 2003-09-30 | 2004-09-15 | 湖南金健米业股份有限公司 | Method for extracting rice protein from rice |
| CN1552885A (en) * | 2003-12-18 | 2004-12-08 | 曹镜明 | Technology for producing rice starch by enzyme |
| CN1654479A (en) * | 2005-01-19 | 2005-08-17 | 黄磊 | Process for continuous extraction of buckwheat starch, buckwheat protein, flavone, and dietary-fiber from buckwheat |
-
2006
- 2006-07-24 CN CNB2006100196830A patent/CN100436592C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448067A (en) * | 2003-04-28 | 2003-10-15 | 江南大学 | Process of preparing glutathione-enriched wheat-germ water soluble extractive |
| CN1528169A (en) * | 2003-09-30 | 2004-09-15 | 湖南金健米业股份有限公司 | Method for extracting rice protein from rice |
| CN1552885A (en) * | 2003-12-18 | 2004-12-08 | 曹镜明 | Technology for producing rice starch by enzyme |
| CN1654479A (en) * | 2005-01-19 | 2005-08-17 | 黄磊 | Process for continuous extraction of buckwheat starch, buckwheat protein, flavone, and dietary-fiber from buckwheat |
Non-Patent Citations (2)
| Title |
|---|
| 颗粒状水可溶性淀粉的新法制备及其结构初步研究. 王洋，眭红卫，刘廷国等.铸造，第54卷第11期. 2005 |
| 颗粒状水可溶性淀粉的新法制备及其结构初步研究. 王洋，眭红卫，刘廷国等.铸造，第54卷第11期. 2005 * |
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