CN1597702A - Method of extrating bela dextran from highland barley - Google Patents
Method of extrating bela dextran from highland barley Download PDFInfo
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Abstract
A process for extracting beta-glucosan from highland barley incldues such steps as pulverizing the grains of highland barley, adding water and extractant, extracting, centrifugal separation, collecting supernatant liquid, heating for removing protein from it, adding precipitant, depositing, and spray drying of deposit.
Description
Technical field
The present invention relates to a kind of method of from cereal, extracting beta-glucan.
Background technology
Beta-glucan is a kind of solubility foodstuff fibre, and grass, in barley, oat, highland barley, beta-glucan is the main component that constitutes the albuminous cell wall in the seed, and almost distributes in whole seed, especially with the content in the wheat bran for the highest.The test of animal and human's body shows that beta-glucan has the effect of tangible reducing blood-fat, control blood cholesterol levels.Different biomaterials is because the difference of its constituent, cause the quality extracted different with efficient, and wherein some barley variety has than paddy rice, wheat, corn, the contour a lot of beta-glucan content of oat.Initial analysis shows that the highland barley in Chinese Tibet has higher beta-glucan content and other functional component, for example contain element and vitamins B and vitamin complexes such as abundant Ca, Fe, Mn, Cn, Al, Mo, and beta-glucan content 〉=6% in the general highland barley (hull-less barley), promptly the mass percent of the beta-glucan in the highland barley accounts for more than 6% of whole highland barley seed.This shows that from barley, especially extract health care composition beta-glucan from highland barley, DEVELOPMENT PROSPECT is very big.
On extractive technique, the method that Chinese patent 97199439.0 discloses a kind of beta-glucan product and extracted from cereal, mainly be that cereal is mixed with water, form slip, then the aqueous solution is separated with solid residue, from the aqueous solution, reclaim beta-glucan, and do not make the step of one or more enzyme deactivations in the method.The raw material of use, therefore will be implemented in and guarantee in the final beta-glucan product that it is very difficult that high beta-glucan content is arranged because wheat bran is the highest structure division of beta-glucan content in the barley for having removed the barley meal of wheat bran but in the method.
Chinese patent 99812477.x discloses a kind of method of producing water-soluble beta-glucan composition from the oatmeal component, wherein composition has the weight ratio of high beta-dextran/glucose, preferable is 15: 1 or higher, used beta-amylase in this method, its consumption is enough to the starch with 50% contained in oatmeal component weight, and preferably the starch of 65% weight is converted into maltose.On production method, need carbohydrate degrading enzyme and beta-amylase in the deactivation raw material, and other Starch debranching enzyme.
Summary of the invention
For the method for beta-glucan is extracted in foundation from the barley highland barley, and improve the content that extracts beta-glucan in yield and the product, the invention provides following technical scheme.
From highland barley, extract the method for beta-glucan, it is characterized in that comprising following steps:
A. pulverize the highland barley seed,
B. add water mixing highland barley seed after crushed, add extraction promotor, extraction,
C. supernatant liquor is collected in centrifugation,
D. remove the protein in the supernatant liquor,
E. add precipitation agent in supernatant liquor, precipitation gets crude extract,
F. spraying drying crude extract.
Preferred scheme is that cereal seed is pulverized through 30～400 orders among the step a.More preferably 150～400 orders.200 orders most preferably.
Preferred scheme is to add extraction promotor in extraction process.The extraction promotor that preferred scheme is added in extraction process is (A) amylase or (B) mixture of zytase, PME, PGs, PLs or mixture (A) and (B), or (A), (B) and (C) Ca
2+, the monascus ruber extracting solution the mixed together thing, addition is 100ml/200kg (quite 3U) highland barley for each components contents.Most preferred scheme for the extraction promotor of adding be the mixture of (A), (B) or (A), the mixture of (B), (C).
Promptly preferred extraction promotor of adding is the mixture of amylase or zytase, PME, PGs, PLs, or is the mixture of amylase, zytase, PME, PGs, PLs, again or amylase, zytase, PME, PGs, PLs, Ca
2+, the monascus ruber extracting solution mixture, addition is 100ml/200kg (quite 3U) highland barley for each component.Most preferably the extraction promotor that Tian Jias is the mixture of amylase, zytase, PME, PGs, PLs or amylase, zytase, PME, PGs, PLs, Ca
2+, the monascus ruber extracting solution mixture, the amount of interpolation is 100ml/200kg highland barley (quite 3U) for each component.
Preferred scheme is for extracting solid-liquid phase ratio for being not more than 1: 1 in extraction process.Preferred extraction solid-liquid phase ratio is 1: 1～1: 5.Most preferred extraction solid-liquid phase ratio is 1: 3～1: 5.
Preferably in extraction process, stir.Preferred scheme is 60～200rpm for the speed that stirs.
The temperature that preferably extracts in extraction process is 30～80 ℃, and the extraction time is more than 2 hours.Preferred scheme is 2～8 hours for the extraction time.Most preferably the extraction time is 4～8 hours.
The speed that preferred scheme is step c centrifugation is 500～5000g.Preferred speed is 1000～3000g.Most preferred speed is 1000g.
Steps d is preferably removed protein with the precipitation of heating (70～100 ℃), filtering method.Preferred temperature is 90～100 ℃.
Preferred scheme is, the precipitation agent that adds in supernatant liquor among the step e is 95% edible ethanol, and the ratio of precipitation agent and supernatant liquor is 1: 0.5～1: 5, and preferred ratio is 1: 1～1: 2, temperature of precipitation is 4～25 ℃, and preferred temperature is 4～15 ℃.Sedimentation time 5～24 hours.Precipitation mode can be natural sedimentation, also can be centrifugation, preferred natural sedimentation.And centrifugation post precipitation or the spray-dried again crude extract that obtains can be used for the processing of health functional food (as highland barley biscuit), food (or feed) additive, medicine intermediate, industrial chemicals, and the working method of use can be conventional working method.
Preferably behind step e, the supernatant liquor behind the collecting precipitation distills and reclaims, and precipitation agent recycles.Supernatant liquor behind the volatile matter distillation precipitation agent (containing a spot of beta-glucan crude extract) can be used for the production of animal-feed, or is used to prepare food, beverage.Highland barley tea or the like for example.
Spray-dired temperature is 200～400 ℃ among the preferred steps f, flow 20～50L/h.
In addition, can also carry out essence to the isolate that obtains behind the step f and carry, the step that essence is carried is
A. dissolve crude extract again,
B. essence is carried,
C. lyophilize.
Usually the smart extracting method of taking can be known technological methods such as column chromatography, membrane filter method.
It can be repeated multiple times that the essence that crude extract is carried out is carried.
We find by a large amount of experiments, and following factor is to influence the major cause that highland barley Beta-dextran extracts quality and extraction yield.
1. the smashing fineness of highland barley is to the influence of extraction yield.
In principle, thinner particle more helps extraction, through we think that usually at smashing fineness be 30 orders when above after the experiment relatively, just can obtain beta-glucan, and particle is thin more, and the beta-glucan extraction yield is high more, sees table 1 for details.
The different smashing fineness of table 1 are to the influence of beta-glucan extraction yield (%)
Smashing fineness 30 orders 60 orders 100 orders 150 orders 200 orders 300 orders 400 orders
Extraction yield % 0.4 ± 0.01 0.6 ± 0.01 1.1 ± 0.02 1.7 ± 0.02 2.3 ± 0.02 2.3 ± 0.04 2.3 ± 0.05
2. extraction solid-liquid phase ratio is to the influence of extraction yield
Solid-liquid phase ratio difference, its extraction yield is also variant, and experimental result shows, is higher than at 1: 1 o'clock in solid-liquid phase ratio, and the beta-glucan extraction yield is lower.Extraction solid-liquid phase ratio (solute and solution than) can obtain higher beta-glucan extraction yield at 1: 1～1: 5 or when lower.But consider that operability in the extraction process and ratio cross the low production unit that can make and increase, production cost increases, and is 1: 1～1: 5 so we preferably extract solid-liquid phase ratio, and preferred extraction solid-liquid phase proportional range is 1: 3～1: 5.Different extraction solid-liquid phase ratios see table 2 for details to the influence of beta-glucan extraction yield.
Table 2 different solid phase ratio is to the influence of beta-glucan extraction yield
Solid-liquid phase ratio 1: 11: 21: 31: 41: 5
Extraction yield % 1.7 ± 0.02 2.3 ± 0.02 2.3 ± 0.06 2.3 ± 0.05 2.4 ± 0.09
3. the development of different extraction promotor and to the influence of extraction yield.
Cell walls characteristic at cereal, by a large amount of explorations and comparative experiments, the extraction promotor that highland barley Beta-dextran extracts has been developed in invention, find to add extraction promotor and can greatly improve cellular segregation and increase softening of the cell wall loose, promote separating of other compositions on beta-glucan composition and the cell walls.Found through experiments, different extraction promotor is seen table 3 for details to the difference that influences of beta-glucan extraction yield.Mainly contain following several extraction promotor and proportioning thereof, (A) amylase, (B) zytase, PME, PGs, PLs, (C) Ca
2+, the monascus ruber extracting solution.
The different extraction of table 3 promotor is to the influence of beta-glucan extraction yield
Extraction accelerant C K A B AB ABC
Addition
(each composition divides the blue or green 100ml/200kg of the blue or green 100ml/200kg of 0 100ml/200kg blue or green 100ml/200kg green grass or young crops
Not) highland barley highland barley highland barley highland barley
Extraction yield % 0.8 ± 0.03 1.5 ± 0.03 1.8 ± 0.08 2.0 ± 0.04 2.3 ± 0.02
Annotate: CK is the blank group.
4. extraction time is to the influence of beta-glucan extraction yield.
Generally speaking, the extraction time is long more, and the beta-glucan extraction yield is high more.When the extraction time more than 2 hours the time, generally just can obtain higher beta-glucan extraction yield, preferred in the present invention 30～80 ℃ of temperature condition as extraction, consider that the long extraction time can improve the technology cost, thus we preferably the extraction time be 2～8 hours, the preferred extraction time is 4～8 hours, in this scope, along with the prolongation of time, extraction yield increases, but the increase of extraction yield is not obvious.See table 4 for details.
Table 4 extraction time (h) is to the influence of beta-glucan extraction yield
Extraction time h 2468
Extraction yield % 2.1 ± 0.04 2.3 ± 0.02 2.3 ± 0.04 2.3 ± 0.01
5. centrifugal speed is to the influence of beta-glucan extraction yield.
The increase of centrifugal speed when separating, the extraction yield of beta-glucan improves, but along with the raising of centrifugal speed can bring a bigger problem, and the beta-glucan purity that is exactly extract is because sneaking into of other materials such as starch and sharply descending.Therefore, take all factors into consideration the processing purposes of its extract, show by assay, centrifugal speed when 500～1000g, though its extraction yield a little less than centrifugal speed 2000g and 3000g, its purity (20%～30%) is significantly higher than latter's (about 10%).Each centrifugal speed sees table 5 for details to the influence of beta-glucan extraction yield.
Table 5 centrifugal speed (g) is to the influence of beta-glucan extraction yield
Centrifugal speed g 500 1,000 2,000 3,000 5000
Extraction yield % 2.1 ± 0.05 2.3 ± 0.02 2.4 ± 0.04 2.4 ± 0.05 2.5 ± 0.09
The method of extracting beta-glucan from highland barley disclosed in this invention is also applicable to various cereal, as barley, wheat, triticale, paddy rice, etc., be particularly useful for barley and highland barley.
Beta-glucan assay employing McCleary etc. (1985,1991) propose and establish, and, the product that extracts purifying is measured by the method that the approval of U.S. cereal chemistry association is recommended, its beta-glucan content can reach 20%～50%, reaches 50～80% after the smart purification; By repeatedly smartly carry, essence after activation and the lyophilize its beta-glucan content of product of purifying can reach more than 90%.
With the contrast experiment of open method in, we find that beta-glucan is present in the highland barley bran in a large number, if will extract after the wheat bran removal again, beta-glucan extraction yield (pressing seed calculates) descends greatly, can't obtain the beta-glucan extractive technique of high extraction.
Figure of description
The technical process that Fig. 1 extracts for highland barley Beta-dextran.
Embodiment
Embodiment 1
Get the highland barley seed, clean the back and reject impurity such as grit, the seed that will clean again after the removal of impurity is pulverized, smashing fineness is 200 orders, adding water in 1: 3 solid-liquid phase ratio mixes, and the ABC that adds the 100ml/200kg highland barley extracts promotor, extraction is 6 hours under 30～80 ℃ of conditions, in the extraction process propeller agitation, stirring velocity 60rpm, centrifugation extraction gained solution, centrifugal speed 1000g, supernatant liquor is collected in centrifugal back, and sedimentary recrement is collected processing through conventional working method can make feed.Supernatant liquor is through materials such as the precipitation place to go protein of heating; Add 95% edible ethanol as precipitation agent with 1: 1 ratio, stir, 4～15 ℃, precipitation obtained crude extract more than 12 hours.Collect supernatant liquor, precipitation agent is reclaimed in distillation in distillation tower, recycles.Supernatant liquor behind the volatile matter distillation precipitation agent (containing a small amount of beta-glucan crude extract) can be used for preparing Tibet highland barley tea or other food, beverage.Get crude extract 200 ℃ of spraying dryings, mist flow is 20L/h, gets finished product beta-glucan crude extract.At this moment, employing McCleary etc. (1985,1991) the beta-glucan content assaying method of proposition and establishment records, beta-glucan content is for being 20～50%, dissolve crude extract once more, adopt the column chromatography essence to carry, the beta-glucan content that records after the lyophilize in the extract is 50%～80%, so repeated multiple times is carried out essence and is carried, and records that beta-glucan content can reach 90% in the end product.
In preparation technology, the one-to-one relationship of beta-glucan content and yield sees table 6 for details in each processing parameter of conversion and the extract.
Embodiment 2
The highland barley barley seed is cleaned the back reject impurity such as grit, remove wheat bran again, after the pulverizing, treatment process is with embodiment 1.The one-to-one relationship of beta-glucan content and yield sees table 7 for details in each processing condition and the extract.
The complete highland barley barley of table 6 is implemented the corresponding relation of beta-glucan content and yield in each processing parameter and the extract in preparation technology
| Embodiment | Smashing fineness (order) | Extraction stirring velocity (rpm) | Extraction promotor | Extraction solid-liquid phase ratio (w/v) | The extraction time (h) | Extraction temperature ℃ | Centrifuge speeds (g) | The ratio of precipitation agent and supernatant liquor (v/v) | Precipitation temperature (℃) | Sedimentation time (h) | Precipitation agent recycles | Spray-dired temperature (℃) | Spray-dired flow (L/h) | β-glucan content measured value | Yield (%) | β-glucan content after essence is carried (%) |
| 1 | 30 | ?60 | ?ABC | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 0.4± 0.01 | ?11.25 | / |
| 2 | 60 | ?60 | ?ABC | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 0.6± 0.01 | ?13.33 | / |
| 3 | 100 | ?60 | ?ABC | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.1± 0.02 | ?24.44 | / |
| 4 | 150 | ?60 | ?ABC | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.7± 0.02 | ?37.78 | / |
| 5 | 200 | ?60 | ?ABC | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 2.3± 0.02 | ?51.11 | 80.1 |
| 6 | 300 | ?60 | ?ABC | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 2.3± 0.04 | ?51.11 | 75.2 |
| 7 | 400 | ?60 | ?ABC | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 2.3± 0.05 | ?51.11 | 73.3 |
| 8 | 200 | ?60 | ?ABC | ?1∶1 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.7± 0.02 | ?37.78 | / |
| 9 | 200 | ?60 | ?ABC | ?1∶2 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 2.1± 0.02 | ?46.67 | / |
| ??10 | ??200 | ??60 | ??ABC | ??1∶3 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.3± ??0.06 | ??51.11 | ??/ |
| ??11 | ??200 | ??60 | ??ABC | ??1∶4 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20h | ?2.3± ??0.05 | ??51.11 | ??/ |
| ??12 | ??200 | ??80 | ??ABC | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.3± ??0.09 | ??51.11 | ??79.1 |
| ??13 | ??200 | ??60 | ??/ | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?0.8± ??0.03 | ??17.78 | ??81.1 |
| ??14 | ??200 | ??60 | ??A | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.5± ??0.03 | ??33.33 | ??/ |
| ??15 | ??200 | ??60 | ??B | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.?8± ??0.08 | ??40.00 | ??/ |
| ??16 | ??200 | ??60 | ??AB | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.0± ??0.04 | ??44.44 | ??/ |
| ??17 | ??200 | ??90 | ??ABC | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.4± ??0.02 | ??53.33 | ??78.3 |
| ??18 | ??200 | ??60 | ??ABC | ??1∶5 | ??2 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20h | ?2.1± ??0.04 | ??46.67 | ??/ |
| ??19 | ??200 | ??100 | ??ABC | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.3± ??0.05 | ??51.11 | ??/ |
| ??20 | ??200 | ??60 | ??ABC | ??1∶5 | ??6 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.3± ??0.04 | ??51.11 | ??/ |
| ??21 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.3± ??0.01 | ??51.11 | ??76.3 |
| ??22 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??500 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.1± ??0.05 | ??46.67 | ??81.4 |
| ??23 | ??200 | ??120 | ??ABC | ??1∶5 | ??8 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.4± ??0.01 | ??53.33 | ??78.2 |
| ??24 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??2000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.4± ??0.04 | ??53.33 | ??/ |
| ??25 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??3000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.4± ??0.05 | ??53.33 | ??/ |
| ??26 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??5000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.5± ??0.09 | ??55.55 | ??69.5 |
Annotate: yield is calculated with the % of the total beta-glucan content of seed.
Table 7 highland barley barley goes behind the wheat bran in preparation technology, implements the corresponding relation of beta-glucan content and yield in each processing parameter and the extract
| Embodiment | Smashing fineness (order) | Extraction stirring velocity (rpm) | Extraction promotor | Extraction solid-liquid phase ratio (w/v) | The extraction time (h) | Extraction temperature ℃ | Centrifuge speeds (g) | The ratio of precipitation agent and supernatant liquor (v/v) | Precipitation temperature (℃) | Sedimentation time (h) | Precipitation agent recycles | Spray-dired temperature (℃) | Spray-dired flow (L/h) | β-glucan content measured value | Yield (%) | β-glucan content after essence is carried (%) |
| 1 | ?200 | ?60 | ?ABC | ?1∶1 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.3± 0.02 | ?28.89 | ?/ |
| 2 | ?200 | ?60 | ?ABC | ?1∶2 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.7± 0.02 | ?37.78 | ?/ |
| 3 | ?200 | ?60 | ?ABC | ?1∶3 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.8± 0.06 | ?40.00 | ?/ |
| 4 | ?200 | ?60 | ?ABC | ?1∶4 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.8± 0.05 | ?40.00 | ?/ |
| 5 | ?200 | ?80 | ?ABC | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.9± 0.09 | ?42.22 | ?/ |
| 6 | ?200 | ?60 | ?/ | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 0.7± 0.03 | ?15.56 | ?75.1 |
| 7 | ?200 | ?60 | ?A | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.3± 0.03 | ?28.89 | ?/ |
| 8 | ?200 | ?60 | ?B | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.5± 0.08 | ?33.33 | ?/ |
| 9 | ?200 | ?60 | ?AB | ?1∶5 | ?4 | ?45 | ?1000 | ?1∶1 | ?10 | ?12 | Be | 250 | ?20 | 1.7± 0.04 | ?37.78 | ?/ |
| ??10 | ??200 | ??90 | ??ABC | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.9± ??0.02 | ??42.22 | ??/ |
| ??11 | ??200 | ??60 | ??ABC | ??1∶5 | ??2 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.7± ??0.04 | ??37.78 | ??/ |
| ??12 | ??200 | ??100 | ??ABC | ??1∶5 | ??4 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.8± ??0.05 | ??40.00 | ??/ |
| ??13 | ??200 | ??60 | ??ABC | ??1∶5 | ??6 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.8± ??0.04 | ??40.00 | ??/ |
| ??14 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.7± ??0.01 | ??37.78 | ??/ |
| ??15 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??500 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.6± ??0.05 | ??35.56 | ??79.99 |
| ??16 | ??200 | ??120 | ??ABC | ??1∶5 | ??8 | ??45 | ??1000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.9± ??0.01 | ??42.22 | ??/ |
| ??17 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??2000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?1.9± ??0.04 | ??42.22 | ??/ |
| ??18 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??3000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.0± ??0.05 | ??44.44 | ??/ |
| ??19 | ??200 | ??60 | ??ABC | ??1∶5 | ??8 | ??45 | ??5000 | ??1∶1 | ??10 | ??12 | Be | ??250 | ??20 | ?2.0± ??0.09 | ??44.44 | ??68.5 |
Annotate: the % of the total beta-glucan content of seed calculates yield before the wheat bran to go.
Claims (20)
1. from highland barley, extract the method for beta-glucan, it is characterized in that comprising following steps:
A. ground cereal seed,
B. add water mixing cereal seed after crushed, extraction,
C. supernatant liquor is collected in centrifugation,
D. the removal protein of heating,
E. add precipitation agent in supernatant liquor, precipitation gets crude extract,
F. spraying drying crude extract.
2. the method for extracting beta-glucan from highland barley as claimed in claim 1 is characterized in that cereal seed is pulverized through 30～400 orders among the step a.
3. the method for extracting beta-glucan from highland barley as claimed in claim 2 is characterized in that cereal seed is pulverized through 200 orders among the step a.
4. the method for extracting beta-glucan from highland barley as claimed in claim 1 is characterized in that adding extraction promotor in extraction process.
5. the method for from highland barley, extracting beta-glucan as claimed in claim 4, it is characterized in that the extraction promotor of adding is (A) amylase or (B) mixture or (A) and mixture (B) of zytase, PME, PGs, PLs in extraction process, or (A), (B) and (C) Ca
2+, the monascus ruber extracting solution the mixed together thing, addition is the 100ml/200kg highland barley for each components contents.
6. the method for extracting beta-glucan from highland barley as claimed in claim 5 is characterized in that the extraction promotor of adding is (A) (B) mixture of (C) in extraction process, addition is the 100ml/200kg highland barley for each component concentration.
7. the method for extracting beta-glucan from highland barley as claimed in claim 1 is characterized in that extracting solid-liquid phase ratio for being not more than 1: 1.
8. the method for extracting beta-glucan from highland barley as claimed in claim 7, it is characterized in that extracting solid-liquid phase ratio is 1: 3～1: 5.
9. the method for extracting beta-glucan from highland barley as claimed in claim 1 is characterized in that stirring in the process of extraction.
10. the method for extracting beta-glucan from highland barley as claimed in claim 9 is characterized in that the speed that stirs is 60～200rpm.
11. the method for extracting beta-glucan from highland barley as claimed in claim 1 is characterized in that the temperature that extracts is 30～80 ℃, the extraction time is more than 2 hours.
12. the method for extracting beta-glucan from highland barley as claimed in claim 11 is characterized in that the extraction time is 4～8 hours.
13. the method for extracting beta-glucan from highland barley as claimed in claim 1, the speed that it is characterized in that step c centrifugation is 500～5000g.
14. the method for extracting beta-glucan from highland barley as claimed in claim 13, the speed that it is characterized in that centrifugation is 1000g.
15. the method for extracting beta-glucan from highland barley as claimed in claim 1 is characterized in that with heating to 70～100 ℃ of postprecipitations, filtering method removal protein.
16. the method for extracting beta-glucan from highland barley as claimed in claim 15 is characterized in that heating to 90～100 ℃.
17. the method for from highland barley, extracting beta-glucan as claimed in claim 1, it is characterized in that the precipitation agent that adds is 95% alcohol in supernatant liquor, the ratio of precipitation agent and supernatant liquor is 1: 1～1: 2, and temperature of precipitation is 4～15 ℃, sedimentation time 5～24 hours.
18. the method for extracting beta-glucan from highland barley as claimed in claim 1 is characterized in that behind step e, the supernatant liquor behind the collecting precipitation, and precipitation agent is reclaimed in distillation, recycles.
19. the method for extracting beta-glucan from highland barley as claimed in claim 1 is characterized in that spray-dired temperature is 200～400 ℃ among the step f, flow 20～50L/h.
20. as each described method of extracting beta-glucan from highland barley in the claim 1 to 19, it is characterized in that can also carry out essence to the crude extract that obtains behind the step f carries, the step that essence is carried is:
A. dissolve crude extract again,
B. essence is carried,
C. lyophilize.
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| CNB2004100537408A CN1331889C (en) | 2004-08-13 | 2004-08-13 | Method of extrating bela dextran from highland barley |
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| CNB2004100537408A CN1331889C (en) | 2004-08-13 | 2004-08-13 | Method of extrating bela dextran from highland barley |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100434442C (en) * | 2006-06-26 | 2008-11-19 | 甘肃奇正藏药有限公司 | Method for enriching beta glucan from highland barley |
| CN101555294B (en) * | 2009-05-14 | 2011-05-11 | 河南工业大学 | Method for extracting highland barley Beta-dextran and dietary fiber by combined-enzyme method |
| CN103355725A (en) * | 2013-07-17 | 2013-10-23 | 西藏青稞研究与发展中心 | Processing technology for hulless barley juice beverage |
| CN104012877A (en) * | 2014-06-25 | 2014-09-03 | 叶炳年 | Preparation method of sweet pickled highland barley |
| CN104286687A (en) * | 2014-10-11 | 2015-01-21 | 天津北洋百川生物技术有限公司 | Method for preparing beta-glucan malt extract |
| CN105707895A (en) * | 2016-01-25 | 2016-06-29 | 朱喜艳 | Hulless barley enzyme beta-glucan and hulless barley enzyme beta-glucan propagation method |
| CN108866122A (en) * | 2018-07-02 | 2018-11-23 | 青海华实科技投资管理有限公司 | A kind of method of water-soluble dietary fiber in extraction highland barley bran |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI94015C (en) * | 1993-06-04 | 1995-07-10 | Exavena Oy | Method for enriching soluble dietary fiber |
| US6426201B1 (en) * | 1996-09-25 | 2002-07-30 | Gracelinc Limited | β-glucan products and extraction processes from cereals |
| DE60133632T2 (en) * | 2000-02-07 | 2009-05-28 | Granate Seed Ltd. | PROCESS FOR EXTRACTION OF G (B) -GLUCAN FROM CEREALS AND PRODUCTS MANUFACTURED THEREOF |
| CA2345606A1 (en) * | 2000-09-27 | 2002-03-27 | The Governors Of The University Of Alberta | Grain fractionation |
-
2004
- 2004-08-13 CN CNB2004100537408A patent/CN1331889C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100434442C (en) * | 2006-06-26 | 2008-11-19 | 甘肃奇正藏药有限公司 | Method for enriching beta glucan from highland barley |
| CN101555294B (en) * | 2009-05-14 | 2011-05-11 | 河南工业大学 | Method for extracting highland barley Beta-dextran and dietary fiber by combined-enzyme method |
| CN103355725A (en) * | 2013-07-17 | 2013-10-23 | 西藏青稞研究与发展中心 | Processing technology for hulless barley juice beverage |
| CN103355725B (en) * | 2013-07-17 | 2016-01-20 | 西藏青稞研究与发展中心 | A kind of highland barley juice drink processing technology |
| CN104012877A (en) * | 2014-06-25 | 2014-09-03 | 叶炳年 | Preparation method of sweet pickled highland barley |
| CN104286687A (en) * | 2014-10-11 | 2015-01-21 | 天津北洋百川生物技术有限公司 | Method for preparing beta-glucan malt extract |
| CN104286687B (en) * | 2014-10-11 | 2017-07-21 | 天津北洋百川生物技术有限公司 | A kind of preparation method rich in beta glucan malt extract |
| CN105707895A (en) * | 2016-01-25 | 2016-06-29 | 朱喜艳 | Hulless barley enzyme beta-glucan and hulless barley enzyme beta-glucan propagation method |
| CN108866122A (en) * | 2018-07-02 | 2018-11-23 | 青海华实科技投资管理有限公司 | A kind of method of water-soluble dietary fiber in extraction highland barley bran |
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