CN1522754A - Extraction of antiviral immune matter for curing sexual disorder and its formulation - Google Patents

Abstract

The present invention selects and uses 48 kinds of pure natural products as raw materials, and uses the lipid active material extracted from 6 raw materials of tea leaf, mulberry leaf, saussurea involucrata, garlic, crataegus fruit and feixianteng through supercritical CO2 extraction process as medicinal carrier and uses pure grain white liquor containing 95 percent alcohol as medium, and adopts the modern biological pharmaceutical technical measures including the following processes: spraying raw material to make anabiosis, freezing membrane, making supercritical extraction, adsorbing and eluting, nano microporous fine filtrating and vacuum freeze-drying so as to obtain the invented medicine preparation. Said invention is capable of resisting virus, raising immunity and curing sexual disorder. Said invention also provides the technique for making said extract into lozenge, capsule, target controllable slowly-released microcapsule and nasal medication preparation.

Description

Antiviral increases the extraction and the preparation of immunization therapy sexual dysfunction thing

The present invention relates to a kind of antiviral and increase extraction of immunization therapy sexual dysfunction thing and preparation technique, specifically, the present invention relates to select for use 48 kinds of pure natural products is raw material, through science compatibility supercritical Co ₂Extraction is extracted lipide actives from 6 kinds of raw materials such as Folium Camelliae sinensis, Folium Mori, Herba Saussureae Involueratae, Bulbus Allii, Fructus Crataegi, forrest silkvine stem or root be pharmaceutical carrier.Is medium with pure grain from making 95 degree Chinese liquor, and by technological design, a kind of antiviral of extraction increases the method for immunization therapy sexual dysfunction thing from raw material.The present invention also specially refers to extract buccal tablet, soft capsule and has the manufacture method of targeting, slow released microcapsule and nasal cavity administrated preparation.

Chinese medicine is the distinctive a kind of national heritage of China, and traditional Chinese herbal medicine of China is the treasure-house of native compound, the development gradually of the centering prodrug complex composition research degree of depth and range in recent years, complex compound in Chinese medicine or the Chinese patent medicine begins to be familiar with by people gradually, the complicated composition that Chinese medicine is not still known, and the undeniable effective complicated mechanism that demonstrates discloses people: the value of Chinese medicine is very high, find that from natural product extract is an importance of new drug research, especially treat the medicine of difficult severe disease and more place hope on natural product, technical solution of the present invention is exactly an achievement in research wherein.

China is aspect the preparation of Chinese patent medicine, basically all adopt traditional high temperature extraction to concentrate, Chinese herbal medicine is mixed the method for getting extract that decocts, some defectives are so just arranged, have in some medical material the efficacy component that is difficult for extracting can not get utilizing fully to extract and run off, and because make up a prescription, the limitation of the site of action of medicine, the dose that is absorbed by the body is very little, drug effect is not good, does not reach intended purposes.For example: the very difficult whole extraction and applications of this flavor Chinese crude drug total Saponin wherein of Radix Ginseng.Thereby tend to make the drug effect inactivation of whole pair of medicine, do not reach expected result.

Study for many years and the clinical trial discovery through the inventor, human body is sick should not regard local effect as, be starting point from the human body aggregate demand, the outstanding pathogenic key that plays a crucial role.As: endocrine hormone is a kind of main intermediary material that transmits information between the cell, act on receptor on the cell membrane of corresponding cell by blood circulation behind many hormone secretions, effect by phosphorylation makes protein phosphorylation then, thereby produces metabolism and other functions that the powerful physical effect is regulated cell in cell.Proteinic phosphorylation also can impel proteinic space conformation to change, and produces the negative interaction of the unusual breeding that promotes cell.Thereby also to keep proteinic dephosphorylation, and could guarantee to make protein to recover original biological attribute, sick is exactly the disequilibrium of proteinic phosphorylation and dephosphorylation.

The traditional Chinese medical science is thought " the kidney being the origin of congenital constitution ", immunology is thought " generation of all diseases all is relevant with gene defect ", dialectical opinion is controlled, organic conception, compound compatibility is the marrow place of modern Chinese medicine development, the metabolism of human body, and anti-disease is a very complicated system, be that the many-sided factor that interknits is facilitated, so, one-sided treatment, control often can not solve root problem, Chinese medicine and pharmacy adopts is still traditional professor's method, bias toward practical experience, it is less to rise to theoretical research, thereby the characteristics of Chinese patent medicine are to suit the remedy to the case, stimulation to patient's integrated mechanism vigor is just few, can only receive the effect of getting half the result with twice the effort.

The present invention is a basic point by above-mentioned cognition exactly, proposes technical solution of the present invention.

Present technique is based on traditional traditional Chinese medical science pharmacology, learns wildly from other's strong points, and selecting 48 kinds of pure natural products for use is raw material, through science compatibility supercritical Co ₂Extraction is extracted lipide actives from 6 kinds of raw materials such as Folium Camelliae sinensis, Folium Mori, Herba Saussureae Involueratae, Bulbus Allii, Fructus Crataegi, forrest silkvine stem or root be pharmaceutical carrier, it improves interior absorption of body of natural activity composition effectively, improve its biological effectiveness significantly, pharmacological action is stronger, and it is lasting rapidly, be medium with pure grain from making 95 degree Chinese liquor again, through the purified extract of 17 procedures.Traditional technique has been broken in its technological design, has created and used the process mechanism of " the origin nutrient substance with life comes back feeding life ".

Medicine combo side is with the design philosophy of " kidney is a basis, regulator gene, the origin of life ", and nucleic acid is the origin material of gene, and decision life gene feature is finished the body metabolism, influence and decision function of human body and healthy core substance; The sufficient gene healthy cell of nucleic acid is active, so designing and selected for use, the inventor produces the medicated beer bacterium yeast that the nucleic acid amount is ranked first in integral medicinal combo side, not only can produce a large amount of nucleic acid, can also synthesize aminoacid, protein, the biological active substances a large amount of with generation, various digestive enzyme, metabolic enzyme, biological endocrine hormone etc. constitute classic nutrient group; But cooperate and the elevator body immunity function with the bacillus bifidus yeast, regulate life, physiological function and the metabolism of human body comprehensively.

The design of pharmacology prescription meets " the kidney being the origin of congenital constitution ", the prescription theory foundation of " gene impairing is ten thousand old complaint sources ", extract is that a kind of nutrition is comprehensive, the pure natural health-care medicine of integrate accent, mend, controlling there is particularly significant curative effect to strengthening body immunity antiviral therapy sexual dysfunction.

The antiviral that the inventive method is extracted increases immunization therapy sexual dysfunction extract and is made up of abundant various active efficacy component, it is responsive especially to acid, alkali, light, heat, air etc., for avoiding in the gastric juice hydrochloric acid and institute's ingesta generation chemical reaction or being decomposed to destroy the extract functional factor is not suffered a loss, guarantee that the efficacy component active matter gives full play to the medical care effect of active ingredient in body.The present invention designs the extract preparation especially to be buccal tablet, soft capsule and to have slow released microcapsule of targeting and nasal cavity administrated preparation, directly is absorbed by blood in the through body by buccal tablet at hypoglossis mucous membrane respectively; Microcapsule is that extract and grease-like pharmaceutical carrier are mixed together and make granular core, wraps one deck coating material on its surface then, the release property of medicine in the control capsule, prolongs drug effect and makes it have targeting, and can be used as the raw material of making multiple dosage form; People's nasal membrane surface area reaches 175 square centimeters, the top layer epithelium has a large amount of microvilluss, and propria lamina blood capillary and lymphatic vessel are abundant, special path is arranged between nasal cavity and cerebrospinal fluid, medicine just enters systemic circulation by Nasal Mucosa Absorption in the administration 5min herein, through clinical trial: in 10～15min, reach maximum drug effect concentration, and only need about 1/10th of oral dose, further avoid hard capsule that the human stomach is had stimulation, cause that uncomfortable reaction and other mode administration cause liver first-pass effect etc.

Purpose of the present invention just provides a kind of antiviral and increases immunization therapy sexual dysfunction thing extracting process and manufacturing process thereof, and it is based on traditional traditional Chinese medical science pharmacology, learns wildly from other's strong points, and selecting 48 kinds of pure natural products for use is raw material, through science compatibility supercritical Co ₂Extraction is extracted lipide actives from 6 kinds of raw materials such as Folium Camelliae sinensis, Folium Mori, Herba Saussureae Involueratae, Bulbus Allii, Fructus Crataegi, forrest silkvine stem or root be pharmaceutical carrier.Is medium with pure grain from making 95 degree Chinese liquor, and through the purified extract of 17 procedures, whole process of preparation is all carried out under low temperature, anaerobic environment, preserves the whole active ingredients and the activity thereof of raw material to the utmost.

The invention provides and from one group of pure natural thing, extract the composition of raw materials that antiviral increases immunization therapy sexual dysfunction thing, a kind of lipid active pharmaceutical carrier that extracts from this group raw material also is provided especially.

Another object of the present invention has provided the manufacture method that a kind of antiviral increases immunization therapy sexual dysfunction extract buccal tablet, soft capsule and has slow released microcapsule of targeting and nasal cavity administrated preparation.

The objective of the invention is to realize by following steps:

A kind of antiviral increases immunization therapy sexual dysfunction thing extraction feed, it is characterized in that: Radix Panacis Quinquefolii, Semen Strychni, Folium Camelliae sinensis, Fu Ling, Folium Bambusae, Rhizoma Polygonati, buckwheat pollen, Radix Glycyrrhizae, royal jelly powder, Semen Cuscutae, Fructus Corni, Radix Polygoni Multiflori, Herba Saussureae Involueratae, Folium Mori, Radix Puerariae, forrest silkvine stem or root, Pericarpium Citri Reticulatae Viride, Hippocampus, Rhizoma Dioscoreae, Radix Salviae Miltiorrhizae, Radix Notoginseng, Lentinus Edodes, Formica fusca, Cordyceps, Flos Caryophylli, Cortex Moutan, He Ye, the Radix Astragali, Cornu Cervi Pantotrichum, thousand pins, ten thousand money grass, Rhizoma Corydalis Decumbentis, Herba Agrimoniae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Alismatis, Auricularia Sophorae, Fructus Crataegi, Herba Cistanches, Fructus Lycii, Herba Epimedii, Rhizoma Chuanxiong, Herb Gynostemmae Pentaphylli, Ganoderma, Radix Rehmanniae, propolis, Bulbus Allii, Flos Lonicerae, yeast, lactobacillus.

Formula proportion of the present invention is as follows:

Radix Panacis Quinquefolii 23 grams, Semen Strychni 1.5 grams, Folium Camelliae sinensis 2.3 grams, volt Siberian cocklebur 0.9 gram, Folium Bambusae 0.3 gram, Rhizoma Polygonati 5.5 grams, pollen of Semen Fagopyri Esculenti 3.7 grams, Radix Glycyrrhizae 5.1 grams, royal jelly powder 15.7 grams, Semen Cuscutae 0.4 gram, Fructus Corni 1.3 grams, Radix Polygoni Multiflori 9.8 grams, Herba Saussureae Involueratae 3 grams, Folium Mori 0.9 gram, Radix Puerariae 62.7 grams, forrest silkvine stem or root 1.5 grams, Pericarpium Citri Reticulatae Viride 46.3 grams, Hippocampus 46.3 grams, Rhizoma Dioscoreae 1.3 grams, Radix Salviae Miltiorrhizae 1 0.2 grams, Radix Notoginseng 3 grams, Lentinus Edodes 0.9 gram, Formica fusca 0.45 gram, Cordyceps 0.8 gram, Flos Caryophylli 15.4 grams, Cortex Moutan 0.9 gram, what leaf 0.4 gram, the Radix Astragali 32.6 grams, Cornu Cervi Pantotrichum 9.4 grams, thousand pins, ten thousand money grass, 15.4 grams, Rhizoma Corydalis Decumbentis 46.3 grams, Herba Agrimoniae 0.6 gram, the Pseudobulbus Bletillae (Rhizoma Bletillae) 0.2 gram, Rhizoma Alismatis 0.9 gram, Auricularia Sophorae 0.3 gram, Fructus Crataegi 0.6 gram, Herba Cistanches 48.6 grams, Fructus Lycii 6 grams, Herba Epimedii 57.4 grams, Rhizoma Chuanxiong 0.3 gram, Herb Gynostemmae Pentaphylli 0.4 gram, Ganoderma 10 grams, Radix Rehmanniae 1.3 grams, propolis 10 grams, Bulbus Allii 5 grams, Flos Lonicerae 10 grams, yeast 1 gram, lactobacillus 1 gram.

Combined formulation describes its pharmacology health care in detail according to after stating, wherein:

1, Radix Panacis Quinquefolii (Radix Panacis Quinquefolii) boosting qi and nourishing yin relieves inflammation or internal heat and promotes the production of body fluid.Contained Radix Panacis Quinquefolii Saponin has resisting fatigue, anoxia enduring, prevention coronary heart disease, arrhythmia, promotes the excretory effect of adrenocortical hormone, is the medicine that strengthens the vitality and the mental status, and the report evidence is arranged, and it has the effect of anti-DNA damnification.

2, Semen Strychni (Semen Strychni Wallichianae) collateral dredging, strong muscle, eliminating stagnation, pain relieving, detumescence.Select this product of therapeutic dose, can suppress cholinesterase activity, discharge acetylcholine (Acety Lcholine)---confirmed to promote the property neurotransmitter on every side of erection function, the effect of loose cavernous body of penis smooth muscle has been arranged.The effect of its another treatment ED is the reflection function of excited spinal cord, strengthens corticocerebral process of excitation, improves the sense of taste, sense of touch, audition, visual performance.

3, its flavor of Folium Camelliae sinensis (Camellia sinensis O.K+ze): sweet, be slightly cold, the refreshment diuresis, relieve heat and thirst.The Main Ingredients and Appearance tea polyphenols, tool antioxidation, anti-inner feelings are transcribed gene regulation always and are had the certain significance, the effect of anticancer, mutation, antithrombotic, antiviral.

4, Fu Ling (Poriacocos) sweet in the mouth, light, the flat diuretic that cures mainly ooze mixedly, contain B-pachyman in the spleen strengthening and blood enriching mind tranquilizing and the heart calming Poria, and pachymic acid protein etc. has slow and persistent diuresis, and calmness and hypoglycemic activity are arranged.

5, contain bioactive substances such as polysaccharide aminoacid and germanium in Folium Bambusae (Phyllos+achys nigya) the Folium Bambusae juice, prove that by experiment it can remove oxygen-derived free radicals, effects such as cancer-resisting.Someone confirms that selenium and polysaccharide exist with the form of selenium polysaccharide in the Folium Bambusae, has higher antioxidant activity when this selenium polysaccharide or polysaccharide individualism.

6, Rhizoma Polygonati (Polygona+um sibiricum Redou+e) sweet in the mouth is flat, the spleen reinforcing lung moistening.

7, pollen of Semen Fagopyri Esculenti (Fagopyrum Csculen+um Moencn) its contain high quality protein, 8 seed amino acids of needed by human, content accounts for 34.75% of total amino acids, wherein lysine accounts for 1.85%, contains VB ₁, VB ₂With enrich zinc, to contain the VE220 microgram be 13 times of soybean oil to every hectogram in the wheat germ oil, mostly 4 times of cod-liver oil are one of food that the known VE of containing is the highest now, are ideal defying age and beauty food.

8, its Main Ingredients and Appearance of Radix Glycyrrhizae (Glycyrrhiza urlensis Fisch): licocoumarone, Radix Glycyrrhizae, light fruit alkene, light fruit profit are decided, and licochalcone A, B and enoxolone etc. have effect to multiple virus, and glycyrrhizic acid also has HIV (human immunodeficiency virus)-resistant activity.

What 9, royal jelly powder (Lac regis apis) (Apiscerana Fabricius) this product was used is honeybee section insecticide, excretory white jelly of worker bee glandulae pharyngeae and the formulated liquid of Mel of apis cerana (Apiscerana Fabricius) etc., it is nutritious, has strengthening by means of tonics, the liver benefiting spleen invigorating, enhancing body resistance, promote growth, promote hemopoietic function, regulate cardiac function and adjust E﹠M function, the application's Lac regis apis lyophilized powder.

10, Semen Cuscutae (Cuscu+achinensis Lam) the kidney invigorating key medicine.It experimental results show that, all contain Zn in the kidney-reinforcing drug, Mn, Cu, trace element and organic compound such as Fe, the nourishing kidney the kidney warming is arranged, strengthening spleen, tonifying kidney, nourishing the liver and kidney, qi and blood tonifying, benefiting essence and marrow, remove free radical, improve the SOD level, radical reaction is to cause one of the many sick inherent important roots of suffering from a deficiency of the kidney, and its warming the kidney to activate YANG medicine trace element is returned the hypothesis through the pattern of theoretical model and warming the kidney to activate YANG medicine trace element " preceding courier ", think that such Chinese medicine returns the essence of kidney, be with trace elements zn, Mn, Fe etc. are as common material base, implement group effect that the control of nerve-endocrine-immunomodulating network is produced, on evidence proof, in the kidney-reinforcing drug when trace element and insufficiency of kidney-YANG, the dependency relation of DNA synthetic ratio abnormal change.

11, Fructus Corni (Comus officinalis siebe+zucc) sweet in the mouth, puckery, warm, liver and kidney tonifying is restrained astringent or styptic treatment for spontaneous sweatingly, and Main Ingredients and Appearance contains cornin, gallic acid etc.The dizzy dizziness, tinnitus, the soreness of the waist and knees that are used for deficiency of the liver and kindey, the sexual impotence of cloudy insufficiency of essence and kidney lose envelope and hide, and Kidney-Yinly thanks to fall from the sky or outer space and pass out semen lingering diarrhea and urinary incontinence, and have antibacterial action.

12, Radix Polygoni Multiflori (Polygonum mul+Yflorum Tbunb) invigorating the liver and kidney, benefiting essence-blood, black beard and hair, bone and muscle strengthening, Radix Polygoni Multiflori Preparata decocting liquid can obviously improve the activity of mice whole blood and cerebral tissue SOD, reduce LPO content in mouse core, liver, the cerebral tissue, the prompting Radix Polygoni Multiflori Preparata can be by improving the removing of the active acceleration bodies inner lipid of body tissue SOD peroxide, reduce the histiocytic infringement of radical pair, up to function in delaying senility.

13, snow is the Herb of feverfew Saussurea medusa Maxim. (Saussurea medusa Maxim) even, removes silt, dries.Its main component is alkaloid, flavonoid, phenols, volatile oil, lactone, steroidal class; Effect with warming the kidney to invigorate YANG, dispelling cold by warming the meridian, warming the lung to resolve fluid-retention, expelling wind and removing dampness.

14, Folium Mori (Morus alba L) dispel the wind, heat clearing away, make eye bright, removing heat from blood, can treat cough due to lung-heat, arteriosclerosis, diabetes, senile dementia etc., Folium Mori have anti-stress, defying age, enhancing body endurance according to the study, reduce cholesterolemia and regulate epinephrine function equivalent fruit, Folium Mori have the effect of stable autonomic nervous system function really.It can reduce the activity of superoxide dismutase in the body, and it is dry moving to alleviate involutional emotion, can reduce the stagnant of lipofuscin in skin or the internal organs (being senile plaque), can also cosmetic hair-care.Contain organic acid---aminoacid such as tartaric acid, citric acid, succinic acid and aspartic acid, glutamic acid, contain globulariacitrin, Pi Su, different Skin glycoside, flavone compound, its cellulose, calcium, sodium, magnesium all than vegetable and fruit Folium Camelliae sinensis content height, also contain VC folic acid, VB ₁, VB ₂And copper, zinc etc., nearest Japanese expert's report wherein contains the functional mass like para-insulin in the Folium Mori, glucose can be relayed to the end from blood and organize slightly and promote sugar to utilize and transform that blood sugar lowering increases effects such as insulin.

15, Radix Puerariae (Pueraria lobasa ohwi) contains multiple active ingredient, has the reduction myocardial oxygen consumption, and arteria coronaria, brain vessel blood amount are increased, obvious allevating angina pectoris, arrhythmia, antioxidation, enhancing human body immunity power, multiple pharmacological effect such as blood sugar lowering.The water solublity of isoflavone compositions such as its Radix Puerariae daidzein, puerarin and fat-soluble all very poor, absorb in the body slowly, bibliographical information is arranged, tablet 1-2 week can produce effects, this can make, and similar pharmaceutical compositions effect prolongs in the prescription (as wants to discharge rapidly, can ethyl cellulose (Ec), Polyethylene Glycol PEG is as the drug-loading system of Radix Puerariae flavone, suitably proportioning gets final product.)

16, forrest silkvine stem or root (Pexiploca forrestii schlechter) is Asclepiadaceae (Asclepi-adaceae) plant, and sweet in the mouth is nontoxic.Adopt clothes, life lengthening, its merit is not little.Cauliflower is controlled all kinds of diseases and ailments, at once the magical effect.This careless deer often eats it, and Gai Luduo is excessive, returns to life from the nether world for the moment, so the name Radix Rhodiolae Yunnanensis.

17, Herb Gynostemmae Pentaphylli (Cayra+ia Japonica Gaga) contains 84 kinds of ginsenoside-Rb _l,-Rb ₃,-Rd ,-F ₂Identical, except that Saponin, also have several amino acids, mineral and vitamin C, trace element reach 20 kinds, and wherein 10 kinds is needed by human, and Herb Gynostemmae Pentaphylli has multiple physiologically active, and tool is anticancer, defying age, antiulcer, analgesia are calmed the nerves effects such as blood fat reducing.China is among the people treats cough, phlegm dyspnea, chronic tracheitis, infectious hepatitis disease with it.Its Se content is very high, and selenium has the protection heart, prevents Keshan disease, Kaschin-Beck disease, hepatopathy and cancer and the anti-ageing function of waiting for a long time.

18, Ganoderma is the Herb after the cleaning, drying of porous section plant Ganoderma lucidum (Leyss. Ex Fr.) Karst. (Ganoderma japonicum (Fh) Lioyd).The effect of tranquilizing by nourishing the heart, relieving cough and asthma, benefiting qi and nourishing blood is arranged, also can be used for the leukopenia that coronary heart disease, hypertension, hyperlipidemia, hepatitis, tracheitis and multiple reason cause, the application's spore powder with crushed sporoderm.

19, Radix Rehmanniae (Renhmannia glu+inosa) has anastalsis, can promote solidifying and cardiotonic of blood, and the equal sweetness and bitterness of property is cold.

20, the hot warm relieving stagnant and resolving masses Shugan Zhitong of Pericarpium Citri Reticulatae Viride (ci+rus re+icula+a Blanco) bitter in the mouth.

21, Hippocampus (Hippocampus Kelloggi Jordan e+snyder) Syngnathidae animal is digested Hippocampus and removes all of epithelium and internal organs, the detumescence of warming the kidney to invigorate YANG knot.

22, Rhizoma Dioscoreae (Dioscorea opposi+a Thunb) is main contains Saponin, choline, protein amylase etc., and certain nutrition and blood sugar lowering effect are arranged, and cures mainly spleen reinforcing antidiarrheal, supports lung tonifying YIN, supplementing the kidney to control the nocturnal.

23, Radix Salviae Miltiorrhizae (Salvia mil+iorrniza Bge) contains Tanshinone I, II, different cryptotanshinone, miltirone, tanshinol I, II etc., it wherein is the representative species of its research with tanshinone, if cause tanshinone sulfonic acid to receive it, be exactly the typical medicaments of carrying out the experiment of monomer TANSHINONES pharmacologically active at present, obtained remarkable result.Radix Salviae Miltiorrhizae has phlegm reduction of blood circulation promoting, functions such as nourishing blood to tranquillize the mind, and the energy coronary artery dilator, coronary blood flow increasing, anoxia enduring improve myocardial metabolism disorder and the functional defect that causes after the anoxia, and anti-congestion effect for reducing blood fat is arranged.

24, Radix Notoginseng (Radix Notoginseng) blood circulation promoting and blood stasis dispelling has antiplatelet aggregation and thrombolytic effect, protection heart and brain, atherosclerosis; Arasaponin, Rb ₁, Rg ₁Can promote maincenter M-cholinoceptor density to significantly improve, significantly increase brain internal protein content; Behind the spinal cord acute injury, can promote the blood confession, microcirculation improvement and play the protection repair; Can obviously increase seminal vesicle weight, have the effect of androgen sample; Can obviously suppress the contractile response that α-adrenoreceptor brings out---suppressed to have the norepinephrine of strong prevention erection effect.

25, Lentinus Edodes (Lentinus edodes (Berk) .sing) belongs to Agaricales, and Tricholomataceae contains outside a large amount of protein, also contains VB ₁, VB ₂, VB ₁₂, VD, calcium, phosphorus, ferrum and 30 plurality of enzymes, 18 seed amino acids etc., especially 8 seed amino acids of human body necessity just have 7 kinds in the Lentinus Edodes.The Lentinus Edodes nature and flavor are sweet flat, and the QI invigorating of being good for the stomach is arranged, and control the wind removing blood stasis, reduce phlegm, function such as puckery urine, be applicable to various cancers, cardiovascular disease, measles; urinary incontinence and diabetes etc., also contain a kind of nucleic acid material in the Lentinus Edodes, can suppress that cholesterol rises in serum and the liver; and can prevent arteriosclerosis, blood vessel becomes fragile and brings high blood pressure down, and anti-angiocardiopathy is had remarkable effect; anti-cold virus; lure into to produce interferon in the body, the protein synthesis of viral interference, thus improve immunity.

26, Formica fusca (Ant) claims " Promethean fire flower ", life lengthening, and rheumatism, anti-hepatitis B, enhancing immunity contains 27 seed amino acids, 19 kinds of enzymes and other useful element of kind more than 20, and antitumor is arranged and control the function of sterile disease.

27, Cordyceps (Cordy ceps sinen sis sacc) sweet in the mouth, temperature, the nourishing the lung and kidney hemostasis and phlegm, it is deficient multiple to cure mainly chronic cough expectorant blood, night sweat sexual impotence, seminal emission, puerperal.

28, Flos Caryophylli (Syzygium aroma+icum Merr e+perry) acrid in the mouth temperature, warming middle-JIAO to send down the adverse QI, warming the kidney to activate YANG can be gone into kidney and enhancing body, it is effective to be used for the treatment of sexual impotence, Main Ingredients and Appearance is an eugenol, promote enterogastric peristalsis, in addition Bacillus typhi, dysentery bacterium, diphtheria corynebacterium and various skin fungus are had inhibitory action.

29, Cortex Moutan (Paeonia Saffru+icosa Andr) acrid in the mouth, hardship, be slightly cold, clearing away heat and cooling blood, promoting blood circulation to remove blood stasis has antibacterial action.

30, He Ye (Nelumbo nucifera Gaer+n) slightly sweet flavor, property are flat, and clearing away heat-damp and promoting diuresis is used for wind syndrome of head, and is dizzy waits disease, and " book on Chinese herbal medicine is new again " recorded and narrated: " refrigerantly separate administration, slake thirst and help produce saliva, control rush down Li, separate intimately, fat-reducing, blood fat reducing, blood pressure lowering etc.

31, the Radix Astragali (Radix Astragali Mongholici) benefiting qi and raising yang, strengthening superficial resistance to stop perspiration, inducing diuresis to remove edema is used for numb limbs and tense tendons, pain, hemiplegia; Can obviously reduce collagen content in aorta and the lung, the prevention of arterial sclerosis raises whole blood superoxide dismutase (SOD), and lipid peroxide obviously reduces; Radix Astragali flavone has the removing oxygen-derived free radicals, and anti-aging effects is arranged.Significantly rising of cyclic adenosine monophosphate (CAMP)---this was the peripheral nerve mediator that promotes erection function to oral administration astragalus in the blood in 20 days, strengthen the loose of cavernous body of penis smooth muscle, the Radix Astragali also contains multiple materials such as choline, aminoacid, folic acid, zinc, potassium, calcium, and is favourable to making smart strong sun.

32, Cornu Cervi Pantotrichum (Cornu Cervi Pantotrichum) kidney invigorating and YANG supporting, benefiting essence-blood, bone and muscle strengthening.Experimental results show that to increase cover ball, Seminal vesicle, levator ani, the weight of spongy body, the activity of inhibition monoamine oxidase, MAO (MAO-B), slow down aging, and can significantly increase dopamine (dopamine) in the brain-a kind of central neurotransmitter, have the ability of good promotion erection.

33, thousand pins, ten thousand money grass (Stellaria Yunnanensis Fr.) sweet in the mouth, slightly warm in nature is mended liver,spleen,kidney.Cloudy blood deficiency is weak, manner is deficient, dizziness, tinnitus, nervous, lumbago foot acid, and walking is difficult, goes into Conception Vessel and Governor Vessel, and treat suffer from a deficiency of the kidney, seminal emission, edema due to deficiency, diseases such as old frequent micturition.Nontoxic, among the peoplely do dietetic therapy.

34, the tuber or the herb of Rhizoma Corydalis Decumbentis (Corydalis decum bens Ders) are bitter cool.Blood pressure lowering pain relieving promoting flow of QI and blood.

35, contain 7.01 milligrams of carotene, VB in the per 100 gram Herba Agrimoniaes of Herba Agrimoniae (Agrimonia Pilosa Ledeb Variaponica) ₂0.63 milligram, the VC157 milligram.Herb also contains agrimonine in addition, agrimonolide etc., Herba Agrimoniae has very strong antitumaous effect, the water-soluble components of its herb has stronger inhibitory action to tumor 5-180 in the mice etc., " Japanese pharmaceutical journal " reports that Herba Agrimoniae extract has stronger inhibitory action to blue cancerous cell, and the mice ehrlich carcinoma is had therapeutic effect, simultaneously, Herba Agrimoniae has 100% promotion growth effect to people's normal cell.

36, the Pseudobulbus Bletillae (Rhizoma Bletillae) (Bie+illa S+ria+a) bitter in the mouth, sweet, puckery, be slightly cold, contain colloid and suppress gram positive bacteria, the lung stomach is lost blood, effective in cure, mainly contain Pseudobulbus Bletillae (Rhizoma Bletillae) glue, the volatilization wet goods has good anastalsis.Can shorten clotting time, and can make red cell agglutination form artificial thrombosis.

37, Rhizoma Alismatis (Alisma Plan+ago aqua+ica L.) has the atheroma of mitigation effect, and obvious to the blood sugar lowering diuresis, it mainly contains compositions such as volatile oil, alkaloid, alisol.

38, Auricularia Sophorae (in the by name Chinese scholartree bolt bacterium Sophora iaponica.L) beginning existing before 1500 " Handbook of Prescriptions for Emergencies " successive dynasties book on Chinese herbal medicine argumentation is all arranged, can " control wind ", "

Blood ", " beneficial power " excessive Qing Dynasty before not surplus 300 year.Confirm through investigation, Auricularia Sophorae is the place of production a kind of medicinal fungi in order to treatment cancer and inflammation among the people, pharmacology proof S180 tumour inhibiting rate is 25%～46%, immunity test has the facilitation of highly significant to macrophage phagocytic function, and is interferon inducted to alpha, gamma, the short NK activity of interferon-alpha has synergism, can improve specific antibody and produce, promote mouse boosting cell DNA synthetic, treatment hepatitis and hepatitis B patient canceration capable of blocking.

39, Fructus Crataegi (Cra+aegus pinna+ifida Bge) blood circulation promoting and blood stasis dispelling; blood fat reducing; prevent that sclerosis of blood vessels and ginkgetin from share; the protective effect for the treatment of myocardial ischemia damage is arranged; think at present; there are a large amount of free radicals to generate during myocardial ischemia, make myocardial cell membrane lipid generation peroxidating, make the myocardial damage aggravation.

40, Herba Cistanches (Cis+anche deser+icola) Main Ingredients and Appearance contains alkaloid and crystallinity neutral substance, and its leachate has hypotensive effect, is used for impotence due to deficiency of the kidney, the seminal emission premature ejaculation.

41, Fructus Lycii (Lycium bar barum) can claim " day essence " replenishing YIN and blood, lung moistening tonifying liver, replenishing vital essence to improve eyesight, cure mainly deficiency of kidney essence, blood sugar lowering, blood pressure lowering, suppress fat and deposit in cell, promote hepatocyte new life, it contains vitamin, chemical compound such as trace element and lycium barbarum polysaccharide, but enhancing immunity, its carotene carotene content is very high, and blood fat reducing is arranged, anticancer change strengthens effects such as intravital cell.

42, Herba Epimedii (Epimedium sagi++aum) acrid in the mouth temperature, the sexual impotence due to being used to suffer from a deficiency of the kidney, seminal emission frequent micturition contain Herba Epimedii glycoside, flavonoid glycoside, lipidol, alkaloid, vitamin E etc. in the Herba Epimedii, the irritability function is arranged, and promote the gonepoiesis effect.

43, the thin gas hero of Rhizoma Chuanxiong (Ligus+icum chuanxiong Hort) flavor, effective in cure to migraine and general headache, arthralgia, fever with chills, active ingredient contains alkaloid, river acid Wei, volatile oil.Analgesia, calmness, relieving convulsion effect are arranged, and the excited respiratory center of energy, blood vessel there is direct dilating effect.

44, propolis (Proplis) is that Apis is gathered extraneous plant sprout bud and secreted natural gum (Gum), resin (Resin), balsam (Balsam) and the oleoresin resinoids such as (oil-resin) in trunk wound, and sneak into Apis salivary gland secretion and the formed a kind of yellowish-brown jelly of Cera Flava (Wax), have broad-spectrum antibacterial and bactericidal action, rich antibiotic, antiviral, biological antioxidant and the anti-tumor activity composition of containing.

45, Bulbus Allii (Allium Staivum) is a liliaceous plant, acrid in the mouth warm in nature, the diuresis of eliminating the phlegm, the parasite killing function of detoxification, active ingredient has inhibition or killing action for big plain and allicin, allicin, flavone compound etc. to escherichia coli, Shigella dysenteriae and deep fungal.

46, Flos Lonicerae (Lonicera japinica Thanb) has another name called Flos Lonicerae, Flos Lonicerae, Flos Lonicerae, Flos Lonicerae, is the dry flower of caprifoliaceae plant Radix Ophiopogonis or the flower of just opening.Cold in nature, the sweet-bitter flavor of Flos Lonicerae has effects for removing toxic heat, and Main Ingredients and Appearance contains chlorogenic acid and isochlorogenic acid, and staphylococcus aureus, streptococcus pyogenes, streptococcus pneumoniae and influenza virus etc. are had inhibitory action.Be mainly used in pyogenic infection amygdala inflammation, respiratory tract infection, enteritis, bacillary dysentery, influenza, parotitis, measles etc.

47, yeast is promptly: and Trichosporon (Trichosporon) beer yeast genus (Brewer ' syeast).

48, lactobacillus promptly: Bifidobacterium (Bifidobadtrum) Streptococcus (Strep-tococcus).

Extraction and preparation process flow process that a kind of antiviral increases immunization therapy sexual dysfunction thing are: film, the ultra-fine freezing and pulverizing of low temperature, supercritical Co are recovered, frozen to raw material preliminary working, spray painting ₂Extract pharmaceutical carrier, the multifunctional integrated lixiviate of medium constant temperature pressure rotation microwave, centrifugalize, filtering residue combo, biotransformation and filtrate, absorb-elute, nanometer micropore fine straining and concentrate, vacuum freeze-drying, the omnipotent pulverizing of topping up nitrogen mix, be processed into Types of Medicine preparation (seeing Fig. 1 for details).

Increasing immunization therapy sexual dysfunction thing extracting process in conjunction with this technological process antagonism virus is described specifically:

A, raw material preliminary working

1) Cornu Cervi Pantotrichum is put into the jacketed pan low temperature sterilization after pulverizing, and puts into container with lid, adds 65% pure grain from making 95 degree high concentration Chinese liquor, stirs, and makes slightly tide sense of Pulveratum Cornu Cervi Pantotrichum, close putting 4 hours, and it is standby that it is fully soaked into.

2) with Bulbus Allii peeling, that the Fructus Crataegi enucleation is broken into the mud shape with common beater grinder is standby.

3) will take advantage of surplus raw material pre-freeze 8 hours under the condition below-3 ℃, be crushed to 60 order powder rapidly with common beater grinder.(seeing Fig. 3, Fig. 4)

4) above-mentioned all raw materials are all mixed, stir.

B, spray painting recovery

With the powder stock of mixing and stirring, joining one lentamente, to stir its rotating speed be 8r/min, spray in the culture tank of 5% normal saline mist vapour, in jar relatively a temperature be 45%, and constant temperature was cultivated 80 minutes for 30 ℃.(pulverized material effect photo after the spray painting recovery is seen Fig. 5)

C, freeze film and low temperature superfine grinding

1) raw material of rapidly above-mentioned processes being crossed was put under-30 ℃ of conditions freezing 8 hours, took out and adopted the Universalpulverizer of topping up nitrogen to be ground into 600 order fine powders, reentered above-mentioned mist vapour sprayed tank and cultivated.

2) repeating above-mentioned spray painting recovers, freezes film, low temperature ultrafine grinding process three times.(the powder material effect photo after three iterative process is seen Fig. 6)

D, supercritical Co ₂The extraction pharmaceutical carrier

To put the supercritical Co of 150 liter capacities through three finished raw materials of iterative process ₂Wait to extract in the layer dish formula stripping vessel, this device mainly is made up of an extractor and two-stage separator, and this extractor and separator all have chuck, available hot water heating and constant temperature, Co ₂Purity should reach 99%, from the Co of steel cylinder ₂After being condensed into liquid, ice chest enters high-pressure pump, the Co after the pressurization ₂Entering extractor after the heater via heating, fully contact with the Powdered extraction feed for the treatment of that wherein soaked into, is that 35 ℃ of pressure are 35Mpa, Co in temperature ₂Flow velocity is to carry out in extraction kettle under the condition of 25kg/h, extracts 5 hours.The treated extraction product (extract lipid pharmaceutical carrier electron micrograph is seen Fig. 8) of winning from extraction kettle, add pure grain in the extraction process from making 95 degree high concentration Chinese liquor, addition is 10 times of the input raw material, the extract of molten Jie in CO 2 fluid, through two road separators, because of pressure reduces, temperature raises, Co in separator ₂Fluid density reduces, and is dissolved in Co in a large number ₂Extract in the fluid be separated finally the extraction product.

Its first separating pressure 10Mpa, 38 ℃ of first separation temperatures, Co ₂Flow velocity is 20kg/h, extracts 40 ℃ of 2 hours second separation temperatures, separating pressure 7Mpa ± 0.4Mpa, Co ₂Flow velocity 15kg/h, 3 hours extraction time, the carbon dioxide after the separation returns ice chest after by effusion meter and can be recycled.

The collection extract will add pure grain through the high speed centrifugation device and separate with extract from making 95 degree high concentration Chinese liquor, and the rotating speed of centrifugal device is 25000r/mim.

E, the multifunctional integrated lixiviate of medium constant temperature pressure rotation microwave

Will be through supercritical Co ₂The dusty raw materials of extraction adds the multifunctional integrated digester (see figure 9) of FQ538-II type medium constant temperature pressure rotation microwave.

1) raw material being put in the extraction pot of 50 liter capacities, is the solvent lixiviate with pure grain from making 95 degree high concentration Chinese liquor, and solvent and raw material ratio are 10: 1.

2) open the digester speed of agitator 8r/mim that flats turn, extracting temperature is 30 ℃ of constant temperature, extracting pressure in jar is 1.2 atmospheric pressures, every two hours starts the 8kw supersonic generator voluntarily and handles 1 hour, and the excusing from death wave producer is the 60 seconds discontinuous dutys in 60 seconds gaps of work.

3) raw material gets lixiviating solution through one Multi Role Aircraft lixiviate 48 hours.

F, centrifugalize

With the centrifugalize of high speed centrifugation device, the centrifugal device rotating speed is that 3000r/min separates multi-functional lixiviating solution, wherein filtering residue combo, biotransformation and filtrate; Filtrate is carried out absorb-elute technologies such as ion exchange resin.

G, filtering residue combo

With the residue of centrifugal removal solution, add pollen of Semen Fagopyri Esculenti through ultra-fine pulverize at low temperature, Lentinus Edodes, Fructus Crataegi each 30%, the water of Mel 10%, yeast 1%, lactobacillus 1%, 8 multiple dose mixes to stir well and spares into reactor.

H, biotransformation and filtrate

1) under 29 ℃ of environment of constant temperature, carried out hermetically sealed biotransformation 48 hours through the material that the stirs biological respinse still of packing into, rotating speed 8r/mim of per 30 minutes mixs, venting in per 3 hours once, sealing continue to transform again, material is that fragrant bent flavor is dense can to carry out ferment filtrate.

2) adopt filter press, carry out the refining filtration of fermentation liquid with 1.5 ‰ ultrafiltration membranes earlier, wash adsorbing to take off behind ferment filtrate and the lixiviating solution mix homogeneously.

I, absorb-elute, to lixiviating solution, ferment filtrate in the following order with the mode absorb-elute that circulates.

1) adopting ion exchange resin that heavy metal lead, hydrargyrum, arsenic etc. are carried out selectivity ion exchange earlier removes.

2) employing is residual to organochlorine pesticide to the adsorbent resin that chlorinated organics has selective absorption, carries out selective absorption and removes.

3) the activated earth adsorbent carries out adsorbing and removing to the sensitization source.

J, nanometer micropore fine straining and concentrated

The employing aperture is that the micropore fine filtration technology of 120 nanometers is carried out the refining filtration of nanometer micropore to extracting solution, fermentation eluent through absorb-elute, through the hollow membrane pervaporation nanometer micropore is made with extra care filtrate simultaneously and concentrates.

K, vacuum freeze-drying

1) will be even through the distilled water stirring of adding 1/5th in the concentrated solution of hollow membrane pervaporation, put into 50 * 30 * 1cm porcelain dish or stainless steel disc, freeze layer thickness and must not surpass 1cm, put into the vacuum freeze drier vaporization chamber.

2) start vacuum freeze drier, temperature in the machine is descended rapidly, cooling rate with-24 ℃/hour drops to-45 ℃, close refrigeration system, begin then water vapour coagulator (4 ℃) evacuation, supply with simultaneously this process of distillation lentamente and divide two stages, the phase I is the process of drying sublimation, the temperature of vacuum freeze drier is risen and maintains 30 ℃.The good vacuum of this stage GPRS, steam pressure link.Its vacuum is in 10u (micrometer of mercury), and vapour pressure will be in 100u (micrometer of mercury), in second stage, when drying sublimation finishes, remains in moisture content in the dry product still about 10%, because the distillation heat dissipation reduces, products temperature rises gradually simultaneously.The maximum temperature that the extract lyophilized powder allows is 35 ℃, and the temperature in dry latter stage will keep 12～14 hours, and the content that just can guarantee remaining moisture content is below 2%, gets final product to such an extent that be spongy vacuum freeze-drying extract.

L, the omnipotent pulverizing of topping up nitrogen and mixing

Vacuum freeze-drying is spongy extract goes into the Universalpulverizer of topping up nitrogen and be ground into fine powder more than 600 orders, promptly get extract of the present invention with lipid active pharmaceutical carrier mix homogeneously again.

The present invention adopts modern medicine biological technique, the extraction preparation process is all carried out under low temperature, anaerobic environment, preserve the whole efficacy components and the activity thereof of raw material to the utmost, the extract preparation has the particularly technique effect of significant curative effect to enhancing immunity antiviral therapy sexual dysfunction, takes human body is not had any untoward reaction and toxic and side effects.

Fig. 1 antiviral increases extraction of immunization therapy sexual dysfunction thing and preparation process flow process.

The macrophage phagocytic that is activated after Fig. 2 administration is removed viral Electronic Speculum and is simulated formula, wherein: 1. by the cell of viral infection; 2. antiviral agent surrounds virus; 3. virus is by antibody blocking; 4. Strain is killed, decomposes; 5. virus is engulfed removing by normal immunocyte.

The comparison of Fig. 3 to Fig. 4 raw material preliminary working pulverize at low temperature.

Fig. 5 raw material is spray painting resuscitation effect photo after pulverize at low temperature.

Fig. 6 raw material is recovered, is frozen film, effect photo after the pulverize at low temperature through spray painting.

Fig. 7 microcapsule---microphotograph (800X) behind the tunicle of extract surface.

Fig. 8 lipid pharmaceutical carrier electron micrograph (1500X).

The multifunctional integrated special equipment FQ538-II of Fig. 9 the present invention type medium constant temperature pressure rotation microwave one body multifunctional digester.Wherein: 1. motor rotary shaft; 2. the medium lixiviating solution passes in and out the mouth of pipe; 3. constant temperature inner layer wall; 4. medium and lixiviate raw material; 5. machine outer wall; 6. add the pressure-volume lid; 7. microwave generator

Figure 10 to Figure 12 erection is the blood flow test comparison before and after administration.

For confirming the effect of invention, the present invention selects respectively kunming mice, normotrophic instar chicken embryos on the 10th etc. to carry out pharmacological testing, function test, toxic and side effect test and clinical trial.

Extract specification requirement of the present invention is as follows:

1. organoleptic indicator: content is that dark brown is Powdered, and little bitterness is without mould spoiled.

2. physical and chemical index: plumbous (mg/kg), in Pb≤0.5

Arsenic (mg/kg) is in As≤0.3

Mercury (mg/kg) is in Hg≤0.3

Moisture (%) is no more than 5%

3. microbiological indicator:

Total plate count (Cfu/g)≤1000

Coliform (MPN/100g)≤40

Mould (Cfu/g)≤25

Yeast (Cfu/g)≤25

Pathogenic bacteria (duodenum 12 road pathogenic bacteria and pathogenic coccus) must not detect.

1, extract antiviral functions test:

Extract is done respectively to suppress influenza virus, herpes simplex types 1 virus, coronavirus, the test of 2 type adenovirus in chicken embryo and small white mouse, the result confirms that extract has the above-mentioned four kinds of virus functions of stronger inhibition and do not find toxic and side effect, and concrete steps are as follows:

1. materials and methods

1.1 material

Being mixed with 10% solution 1.1.1 get extract and distilled water, is 1: 10 with this liquid normal saline dilution again, 1: 100, and 1: 1000 solution for standby, proofreading and correct PH is about 7.4.

1.1.2 be 640 influenza virus A3s/Henan Province health and epidemic prevention station with hemagglutinative titer, herpes simplex types 1 virus/Henan Province's epidemic prevention station, coronavirus/Henan Province health and epidemic prevention station, 2 types celo virus/Henan Province health and epidemic prevention station is respectively with normal saline dilution to 10 times.

1.1.3 instar chicken embryo was 32 in normotrophic 10 days

1.1.4 32 (certificates of competency: the moving word of doctor 10-8004 number) of secondary Kunming kind small white mouse

1.1.5 1% chicken red blood cell liquid

1.1.6 1% mouse red blood cell liquid

1.2 method

1.2.1 the chicken embryo is divided into 8 groups, every group 4, with four kinds of viral test solutions such as influenza virus, herpes simplex types 1 virus, coronavirus, 2 type adenovirus, inject a kind of virus by per 2 groups, inject 0.2ml in the allantois by the every embryo of grouping, paraffin sealing, put into 37 ℃ of incubators, stir chicken embryo twice every day, hatch 72 hours after, the infringement of observation zero accident, optional every group of 3 chicken embryos are put into 4 ℃ of refrigerators.

1.2.2 remaining 8 chicken embryos continue at 37 ℃ of incubations, observe toxic action.

1.2.3 the extraction chick embryo allantoic liquid is done hemagglutination test, measures hemagglutinative titer (second day carries out after the refrigeration).

1.2.4 the secondary small white mouse is divided into 8 groups, 4 every group; 4 groups is that collunarium sucks extract solution, and every 0.25ml gives respectively a kind of virus of every winding kind after 10 minutes; Inoculate first behind every 30min of a kind of virus more every of lumbar injection extract solution 2ml, observe toxic action for other 4 groups every group.

2, result

2.1 8 chick embryo developments of virulence experiment are normal, hatch the chicken well-grown.

2.2 extract solution suppresses influenza A3, herpes simplex types 1 virus, coronavirus, 2 type adenovirus in the chicken embryo.

2.3 small white mouse virulence experiment elder generation collunarium sucks extract solution, inoculates four kinds of viruses, can suppress the breeding of virus and play prevention effect.

2.4 small white mouse virulence experiment elder generation virus inoculation, pathological reaction, well-grown all do not appear in four groups of small white mouses that the 30min pneumoretroperitoneum injects extract solution.

Table one hemagglutinative titer (chicken embryo) table two. hemagglutinative titer (white mouse)

Extract	1∶10	1∶100	1∶1000
				Hemagglutinative titer	- - -	- 1∶20 -	- - -

Extract	1∶10	1∶100	1∶1000
				Hemagglutinative titer	-   -   -	1∶80   -   -	-   -   -

Macrophage phagocytic is removed viral Electronic Speculum and is simulated formula (seeing Fig. 2).

2, extract increases the immunoloregulation function test:

Experimental animal: secondary Kunming mouse, the body weight 18-22g (certificate of competency: No. the 01st-5408, the moving word of doctor)

Extract human body recommended amounts is 6g/60kg, and enlarging 10 times is 1.0g/kg for middle dosage, a upper and lower dosage group 3.0g/kg high dose and the 0.5g/kg low dosage respectively established of test group.

Control group takes distilled water, and gavage 30 days is measured every immune indexes.

1. extract is on the impact of mouse delayed allergy (DTH).

Dinitro stem fluorine stupid (DNFB) is induced abnormal anti-(DTH) method of delayed: the ear swelling method, behind the DNFB sensitized mice, attacked auris dextra with DNFB again on the 5th day, put to death animal behind the 24H and cut left and right sides auricular concha, take off the auricle of lower footpath 8mm with card punch and weigh, represent the degree of DTH with the difference of left and right sides ear weight.

Table three extract is to the mouse cell Immune Function

Group	Dosage (g/kg, bw)	Annotate ConA and induce spleen lymphocyte proliferation N OD difference N	DNFB induces the DTH left and right sides ear method of double differences (mg)
				Control group	0	15   0.033±0.005    15	19.6±2.28
Low dose group	0.5	15   0.044±0.008#   15	23.2±2.70
				Middle dosage group	1	15   0.056±0.011#   15	27.6±2.87
High dose group	3	15   0.042±0.006#   15	22.1±2.15

Annotate: get 0.1 and measure OD570 value control group comparison＜0.05#P＜0.01

By table three as seen, 1. strengthen the spleen lymphocyte proliferation ability that ConA induces with the basic, normal, high dosage group of control group comparison extract conspicuousness.

2. react with the DTH that the basic, normal, high dosage group of control group comparison extract conspicuousness strengthens, mouse DNFB brings out.

3. see Table body weight no significant difference between four each animals

Table four: the weight of animals record before and after the test (gram X ± S)

Group	Dosage (g/kgbw)	Number of animals (only)	Before the test	After the body weight test	Weightening finish (gram)
						Control group	0	15	18.7±2.0	29.0±2.5	10.4±1.8
Low dose group	0.5	15	19.1±1.3	30.7±1.9	11.5±1.5
						Middle dosage group	1	15	19.6±1.6	30.6±2.0	11.0±2.1
High dose group	3	15	18.4±1.6	29.7±2.5	11.1±1.0

4. see Table thymus gland between five each animals/body weight ratio and spleen/body weight ratio no significant difference.

Table five: on the impact of animal lymph organ/body weight ratio (X ± S)

Group	Dosage (g/kgbw)	Number of animals (only)	Thymus gland/body weight ratio (* 10-9)	Spleen/body weight ratio (* 10-9)
					Control group	0	15	3.13×0.49	5.33×0.83
Low dose group	0.5	15	3.20×0.38	5.18×0.68
					Middle dosage group	1	15	3.22×0.41	5.24×0.48
High dose group	3	15	3.32×0.23	5.26×0.44

5. but and control group visible from table six compares the basic, normal, high dosage group of extract preparation conspicuousness rising serum hemolysin content.

Table six: the extract preparation is on the impact of mouse humoral immune function

Group	Dosage (g/kgbw)	Number of animals (only)	Serum hemolysin (antibody product)
				Control group	0	15	92.6±17.48#
Low dose group	0.5	15	150.3±32.69#
				Middle dosage group	1	15	156.9±35.11#
High dose group	3	15	148.7±41.17#

Compare P＜0.05#P＜0.01 with control group

6. from the visible basic, normal, high dosage group of the extract preparation phagocytic percentage of table seven, phagocytic index is all apparently higher than Normal group.

Table seven: the extract preparation is on the impact of Phagocytosis By The Peritoneal Macrophages In Mice

Group	Dosage (g/kgbw)	Number of animals (only)	Phagocytic percentage (%)	Phagocytic index
					Control group	0	15	42.9±4.06	0.449±0.041
Low dose group	0.5	15	51.8±4.21	0.551±0.04#
					Middle dosage group	1	15	56.4±3.44	0.592±0.04#
High dose group	3	15	49.2±3.89#	0.514±0.04#

Compare #P＜0.01 with control group

But 7. improve mouse carbon from the visible basic, normal, high dosage group of the extract preparation conspicuousness of table eight and clean up phagocytic index.

Table eight: the extract preparation is cleaned up function effect to mouse carbon

Group	Dosage (g/kgbw)	Number of animals (only)	Phagocytic index
				Control group	0	15	6.077±0.58
Low dose group	0.5	15	7.671±0.54#
				Middle dosage group	1	15	7.902±0.78#
High dose group	3	15	7.567±0.86#

Compare #P＜0.01 with control group

3, the treatment of sexual dysfunction experiment of increasing property of extract function and clinical trial

1. experimental animal Wistar rat (accurate code moves word 003-57C74 number for the doctor) is selected 80 of body weight 40～60g ablactation rats, is divided at random 4 groups, and 20 every group male.

It is 6g/60kg that the extract human body pushes away storage, and enlarging 10 times is middle dosage 1.0g/kg, a upper and lower dosage group 3.0g/kg high dose and the 0.5g/kg low dosage respectively established of test group.

Control group takes distilled water, and gavage 30 days is surveyed respectively rat testicle/body weight ratio, scrotum/body weight ratios affect experimental result sees Table nine.

Table nine: the extract preparation is on the impact of rat testicle, scrotum/body weight

Group	Dosage (g/kgbw)	Number of animals (only)	Testis/body weight ratio (g/100g)	The P value	Scrotum/body weight ratio (g/100g)	The P value
							Control group	0	20	0.66±0.11		0.28±0.06
Low dose group	0.5	20	0.73±0.12	＜0.05	0.34±0.06	＜0.05
							Middle dosage group	1	20	0.77±0.13	＜0.05	0.35±0.06	＜0.05
High dose group	3	20	0.78±0.16	＜0.05	0.39±0.05	＜0.01

By table nine as seen, basic, normal, high three groups of dosage group testis, scrotum/body weight ratio and water control group have comparison significant difference (P＜0.05, P＜0.05, P＜0.01) can promote the testis weightening finish, and the scrotum weightening finish promotes attached property device to grow.

2. clinical trial:

Select 40 routine male patients, be divided at random 4 groups, every group of 10 people. Divide control group, basic, normal, high three dosage of test group. With nasal cavity nasal drop administration 0.25mg, 0.5mg, 1.0mg. Control group shows with placebo (distilled water) result, only in 5min, medicine just enters systemic circulation by Nasal Mucosa Absorption, reach maximum effect drug effect concentration in 10～15min, the erection success rate is respectively: 86%, 90%, 99%, and finish the sexual intercourse rate and reach: 75%, 90%, 95% and contrast comfort group is invalid.

After tested: (1) penis erecting strength and volume increase, and congested amount increases obviously.

(2) plateau, prolong, and penis is strong, strong.

(3) ejaculate volume once added the orgasm phase, and property is prevented and moved back the after date non fatigue feeling.

(4) telotism cavernous body CBF test effect (seeing Figure 10, Figure 11, Figure 12).

4, extract preparation toxicological experiment

Animal used as test is secondary kunming mice and Wistar rat, accurate code is respectively quality certification doctor moving word: 01-357M06 number, 8707M01 number.

(1) acute toxicity test in mice

20 of animal used as test mouse, each 10 of male and female, body weight is 18～22g, every day twice gavage mouse, dosage is: 20g/kgbw, observe 14d, observe animal poisoning manifestations and death condition.

Experimental result sees Table ten

Table ten: chmice acute toxicity test result

Animal varieties	Sex	By way of	LD 50(g/kg.bw)
				Mouse	Male	Per os	＞20
Mouse	Female	Per os	＞20

Acute, toxicity test result: after gavage gave animal subject, the mouse of two kinds of sexes was showed no obvious poisoning symptom, and animal is without death in the 14d. Therefore see Table ten and think the acute toxicity LD of this tested material to two kinds of sex mouse₅₀All greater than 20g/kg.bw according to acute toxicity grading criteria, the true border of this sample innocuous substance.

(2) genetic toxicity test

(2.1) Salmonella reversion test

Test strain is salmonella typhimurium histidine defect type TA97, TA98, TA100, TA102, the Activated in Vitro system is that the S-9 of the Polychlorinated biphenyls rat liver homogenate preparation of inducing mixes, 250,500,1000,2500, a 5000ug/m5 dosage if, establish from beaming back change group, solvent control group.

The results are shown in Table 11,12.

Table ten one: Salmonella reversion test result (for the first time) (X ± S)

Dosage TA97 TA98 TA100 TA102

(ug/ ware)-s-9+s-9-s-9+s-9-s-9+s-9-s-9+s-9

5000        139±7.1    159±12.0   23±1.4 31±4.2   147±16.9  150±11.3 242±21.2 240±19.7

2500        160±12.7   168±8.4    26±4.2 39±4.2   137±16.9  147±5.6  260±4.2  261±15.5

1000        163±4.2    175±14.8   28±1.4 33±2.8   131±16.3  138±11.3 256±18.3 270±18.3

500         169±4.9    179±8.4    32±4.2 39±2.8   139±13.4  143±8.4  265±11.3 271±22.6

250         166±11.3   173±12.7   34±5.6 36±4.2   144±9.3   149±12.7 276±21.2 274±12.7

Become 130 ± 9.8 136 ± 10.5 30 ± 2.8 34 ± 4.2 136 ± 14.8 134 ± 14.8 278 ± 16.9 285 ± 29.6 from beaming back

Solvent control 138 ± 2.8 147 ± 9.8 35 ± 4.2 38 ± 2.8 135 ± 12.7 139 ± 14.1 273 ± 25.4 282 ± 14.1

Positive control^*  830(1)      907(2)      854(3)  ＞2000(2) ＞2000(4)  ＞1194(2) ＞2000(5) 1202(2)

Table ten two: Salmonella reversion test result (second day) (X ± S)

Dosage TA97 TA98 TA100 TA102

(ug/ ware)-s-9+s-9-s-9+s-9-s-9+s-9-s-9+s-9

5000    144±15.5 161±11.3 28±1.4 32±1.4  144±15.5 159±21.2 260±31.7  260±18.3

2500    151±8.4  165±9.8  29±1.4 32±2.8  148±14.8 154±14.1 267±22.6  270±22.6

1000    167±15.5 178±10.5 35±5.6 34±4.2  147±17.6 158±9.8  268±21.2  284±25.4

500     174±5.6  182±14.1 35±1.4 34±4.2  153±15.5 155±16.9 275±21.2  282±16.9

250     179±8.4  187±2.8  36±2.8 37±4.2  157±9.8  159±11.3 283±18.3  291±25.4

Become 141 ± 10.5 150 ± 9.1 34 ± 5.6 37 ± 2.8 143 ± 12.7 141 ± 14.1 283 ± 24.0 298 ± 22.6 from beaming back

Solvent control 140 ± 2.8 154 ± 9.8 33 ± 2.8 38 ± 4.2 140 ± 16.9 146 ± 15.5 280 ± 15.5 295 ± 15.5

Positive control ^*830 (1) 907 (2) 854 (3)＞2000 (2)＞2000 (4)＞1194 (2)＞2000 (5) 1202 (2)

(2.2) PCEMNR micronucleus test, select 50 of body weight 25-30g mices, be divided into five groups at random, every group 10, male and female half and half, tried in 3 experimental grouies that the agent amount is respectively 2.50,5.00,10.0g/kg.bw, other establishes negative control group and positive controls, (cyclophosphamide, 40g/kb.bw), adopt 24h twice per os administration by gavage in interval to test, last with give tried thing after 6h put to death animal, conventional film-making, 1000 polychromatic erythrocytes of every animal microscopy, record micronucleus cell number calculates micronuclear rates.

The results are shown in Table 13

Table ten three: PCEMNR micronucleus test result

Sex	Dosage (g/kg.bw)	Number of animals	Check cell number	The micronucleus number	Micronuclear rates (‰)
						??♂	2.50	??5	??5×1000	??9	??1.8
5.00	??5	??5×1000	??10	??2.0
					10.0		??5	??5×1000	??9	??1.8
Negative control	??5	??5×1000	??9	??1.8
					Positive control		??5	??5×1000	??146	??29.2 **
??♀	2.50	??5	??5×1000	??10							??2.0
					5.00	??5	??5×1000	??10	??2.0
	10.0	??5	??5×1000	??9						??1.8
					Negative control	??5	??5×1000	??10	??2.0
	Positive control	??5	??5×1000	??155						??31.0 **

^*P＜0.01 (comparing the Poisson distribution inspection) with negative control group.

The PCEMNR micronucleus test is the result show: by table ten three as seen, tried there was no significant difference (P＞0.05) between each dosage group micronuclear rates of thing and the negative control group, and between ring phosphinylidyne ammonia positive controls and the negative control group utmost point significant difference (P＜0.01) is arranged, illustrate that this sample does not have the PCEMNR of causing micronucleus generation effect.

(2.3) mouse sperm deformity test

Select 25 of 25-35g male mices, be divided into 5 groups at random, 5 every group, 3 test group, dosage is 2.50,5.00,10.0g/kg.bw, other establish negative control group and positive controls (ring phosphinylidyne ammonia, 40g/kg.bw), irritate stomach, continuous 5d, the sperm of 1000 structural integrities of every animal counting, distortion type and quantity, calculate rate of teratosperm, result of the test sees Table 12.

Table ten four: mouse sperm deformity experimental result

Dosage (g/kg.bw)		Number of animals	Examined sperm count	The sperm deformity number	Rate of teratosperm (%)
						Test group	??2.50	5	??5×1000	??114	??2.28
??5.00	5	??5×1000	??117	??2.34
					??10.0		5	??5×1000	??116	??2.32
Negative control		5	??5×1000	??127							??2.54
					Positive control		5	??5×1000	??292	??5.84 **

^*P＜0.01 (comparing the X2 check with negative control group).

The mouse sperm deformity result of the test shows: by table ten four as seen, tried there was no significant difference (P＞0.05) between each dosage group mouse sperm deformity rate of thing and the negative control group, and between ring phosphinylidyne ammonia positive controls and the negative control group utmost point significant difference (P＜0.01) is arranged, illustrate that this sample does not have the mouse sperm deformity of causing effect.

(2.4) mouse testis chromosome deformity test:

Select 25 of body weight 30-35g male mices, be divided into 5 groups at random, every group 5, dosage 2.50,5.00,10.0g/kg.bw, other establishes negative control group and positive controls (is encircled phosphinylidyne ammonia, 40g/kg.bw), continuous irrigation stomach 5d, 100 metacinesis phases of microscopy, distortion type and quantity are calculated chromosome aberrations rate.

Result of the test sees Table 15

Table ten five: mouse testis chromosomal aberration test result

Dosage (g/kg.bw)	The observation of cell number	Autosome separates N %	Sex chromosome separates N %	Chromosomal aberration N %
					??2.50	??5×100	??2????????0.4	??13???????2.6	??1???????0.2
??5.00	??5×100	??2????????0.4	??14???????2.8	??1???????0.2
					??10.0	??5×100	??3????????0.6	??9????????1.8	??1???????0.2
Negative control	??5×100	??3????????0.6	??11???????2.2	??1???????0.2
					Positive control	??5×100	??24???????4.8 **	??33???????6.6 **	??34??????6.8 **

^*P＜0.01 (comparing check with negative control group).

The mouse testis chromosomal aberration test is the result show: by table ten five as seen, tried there was no significant difference (P＞0.05) between each dosage group of thing and the negative control group, and significant difference (P＜0.01) is arranged between positive controls and the negative control group, illustrate that this sample does not exert an influence to the mouse testis chromosomal aberration.

(2.5) rat 30d feeding trial

Select 80 of body weight 40-60g ablactation rats, be divided into 4 groups at random, 20 every group, male and female half and half, dosage is 10.0,20.0,40.0g/kg.bw, and per os is irritated stomach every day twice, and matched group is fed normal feedstuff, record rats eating amount, body weight, observes 30d continuously.

Result of the test table ten six, 17

Table ten six: 30d feeds rat body weight influence (g, X ± S)

Sex	Dosage (g/kg.bw)	Starting weight	The 1st week	The 2nd week	The 3rd week	The 4th week
							♂	??0.00	?59.70±3.47	??96.00±8.76	??131.00±8.23	?154.53±12.8	?180.00±6.67
??10.0	?57.50±2.66	??104.5±11.7	??134.00±7.38	?145.50±28.7	?184.50±5.99
						??20.0		?58.00±4.00	??98.50±13.8	??130.00±5.77	?151.50±7.47	?179.00±9.66
??40.0	?57.60±2.76	??101.5±12.3	??130.00±7.07	?151.50±7.47	?181.50±7.47
						♀		??0.0	?59.70±2.33	??81.00±8.43	??111.00±12.2	?130.00±15.6	?157.50±11.8
??10.0	?53.00±3.50	??85.00±4.71	??120.50±5.27	?140.00±7.07	?160.50±8.64
							??20.0	?54.00±5.16	??88.50±7.09	??125.00±6.67	?144.5±5.99	?166.50±7.09
??40.0	?54.50±4.38	??89.50±6.43	??123.00±8.56	?139.50±11.6	?162.00±11.6

Table ten seven: 30d feeds influence to rat total foodstuff utilization rate (g, X ± S)

Sex	Dosage (g/kg.bw)	Weight gain (g)	Food-intake (g)	Food utilization (%)
					??♂	????0.00	??119.30±8.53	????426.00±20.11	????28.00
????10.0	??126.50±6.69	????445.50±23.82	????28.40
				????20.0		??120.90±8.61	????438.00±15.49	????27.60
????40.0	??124.40±7.09	????435.00±19.86	????28.60
				??♀		????0.0	??99.60±13.93	????329.00±37.25	????30.27
????10.0	??107.50±7.91	????348.00±25.30	????30.89
					????20.0	??113.50±6.26	????361.50±37.79	????31.39
????40.0	??106.50±10.01	????359.50±42.39	????29.62

General performance, body weight, food utilization from animal: the refusing to eat phenomenon does not all appear in each experimental group animal the experiment, vegetative activity is normal, body weight and food utilization result see Table 16 and table ten seven respectively, it can be seen from the table there was no significant difference (P＞0.05) between each experimental group the weight of animals and the matched group, also there was no significant difference (P＞0.05) between each experimental group animal food-intake, food utilization and the matched group.

Routine blood test and biochemical indicator:

The results are shown in Table 18,19,20 (1), 20 (2).

Table ten eight: rat 30d feeding trial hematological examination result (X ± S)

Sex	Dosage (g/kg.bw)	Hemoglobin (g/L)	Red blood cell count(RBC) (* 10 12/L)	Numeration of leukocyte (* 10 9/L)
					??♂	????0.00	????143.40±6.19	????6.71±0.35	????10.70±0.21
????10.0	????154.40±4.88	????7.34±0.18	????10.92±0.55
				????20.0		????153.80±5.63	????7.57±0.32	????11.02±1.17
????40.0	????150.60±6.34	????7.40±0.45	????10.00±0.44
				??♀		????0.0	????140.40±9.09	????6.67±0.43	????10.48±0.28
????10.0	????143.20±2.77	????6.95±0.20	????10.80±0.48
					????20.0	????150.00±3.61	????7.42±0.29	????10.82±0.59
????40.0	????149.20±4.32	????7.35±0.35	????11.38±3.12

Table ten nine: 30d feeds the influence to rat leukocyte

Sex	Dosage (g/kg.bw)	Neutral (%)	Lymph (%)	Other (%)
					??♂	????0.00	????17.40	????81.05	????1.55
????10.0	????14.42	????83.84	????1.74
				????20.0		????14.56	????83.48	????1.96
????40.0	????14.50	????83.48	????1.02
				??♀		????0.0	????15.10	????83.24	????1.66
????10.0	????15.00	????83.56	????1.44
					????20.0	????15.64	????83.12	????1.24
????40.0	????15.10	????83.80	????1.26

Table two ten (1): rat 30d feeding trial blood in latter stage seriation result (X ± S)

Sex	Dosage (g/kg.bw)	Glutamate pyruvate transaminase (U/L)	Glutamic oxaloacetic transaminase, GOT (U/L)	Blood urea nitrogen (mmol/L)	Creatinine (mmol/L)	Cholesterol (mmol/L)
							♂	????0.00	??58.17±8.62	??150.43±11.52	??6.97±0.92	??32.15±2.03	??1.47±0.21
????10.0	??65.21±3.58	??146.25±34.82	??7.73±1.04	??31.22±1.79	??1.52±0.21
						????20.0		??57.35±4.77	??134.05±12.05	??7.76±0.61	??29.44±1.80	??1.54±0.11
????40.0	??58.80±5.74	??151.79±37.47	??7.13±0.32	??27.68±1.55	??1.55±0.21
						♀		????0.0	??57.75±15.4	??184.41±18.90	??7.90±1.23	??34.53±4.51	??1.69±0.14
????10.0	??49.59±6.72	??184.86±19.21	??7.29±0.17	??33.65±1.61	??1.60±0.31
							????20.0	??53.43±6.42	??189.87±46.44	??7.49±0.55	??30.03±1.76	??1.58±0.05
????40.0	??56.18±8.83	??169.59±28.09	??7.54±1.50	??33.86±5.14	??1.68±0.26

Table two ten (2): rat 30d feeding trial blood in latter stage seriation result (X ± S)

Sex	Dosage (g/kg.bw)	Triglyceride (mmol/L)	Blood glucose (mmol/L)	Total protein (g/L)	Albumin (g/L)
						??♂	????0.00	??0.92±0.26	??7.40±0.90	??68.60±2.23	??43.29±1.61
????10.0	??0.47±0.15	??7.94±0.67	??74.27±2.84	??46.00±1.27
					????20.0		??0.39±0.11	??7.90±0.57	??76.95±2.98	??46.36±0.99
????40.0	??0.60±0.17	??7.97±0.54	??73.43±3.94	??43.28±2.14
					??♀		????0.0	??0.99±0.33	??7.51±0.54	??76.76±1.72	??46.44±3.21
????10.0	??0.67±0.17	??7.94±1.49	??76.00±5.20	??47.52±1.85
						????20.0	??0.59±0.25	??7.29±0.88	??75.47±3.64	??46.61±2.18
????40.0	??0.95±0..45	??7.49±1.19	??70.26±4.93	??46.64±4.33

Routine blood test and biochemical indicator the results are shown in Table 18,19,20 (1), 20 (2).Indexs such as the it can be seen from the table hemoglobin of each experimental group, erythrocyte sum, numeration of leukocyte and classification thereof, the paddy third ammonia enzyme, glutamic oxaloacetic transaminase, GOT, blood urea nitrogen, creatinine, cholesterol, triglyceride, blood glucose, total protein, albumin are all within normal range, and there are no significant between negative control difference (P＞0.05).

4, the dirty body comparative result of 30d sees Table 21

Table two 11 rat 30d feed dirty body comparative result (X ± S)

Sex	Dosage (g/kg.bw)	Liver/body (%)	Spleen/body (%)	Kidney/body (%)
					??♂	????0.00	????4.75±0.96	????0.34±0.04	????0.98±0.02
????10.0	????4.26±0.19	????0.32±0.01	????0.94±0.01
				????20.0		????4.23±0.16	????0.31±0.02	????0.94±0.01
????40.0	????4.34±0.17	????0.33±0.02	????0.95±0.02
				??♀		????0.0	????4.21±0.31	????0.30±0.02	????0.99±0.01
????10.0	????4.37±0.34	????0.31±0.03	????0.93±0.02
					????20.0	????4.21±0.41	????0.33±0.02	????0.93±0.02
????40.0	????4.22±0.32	????0.33±0.03	????0.94±0.03

Reach histological examination substantially: each experimental group of gross examination of skeletal muscle is no abnormal, and when dissected is not also found bladder, common hepatic duct calculus, organ coefficient no abnormality seen (seeing Table 21).Liver,spleen,kidney, stomach, duodenum, testis or ovary etc. that mirror is observed matched group and experimental group animal down there is no significant pathological change.

Brief summary:

1, acute toxicity test in mice: according to acute toxicity grading criteria, this sample belongs to innocuous substance.

2, genetic toxicity test:

2.1 Salmonella reversion test: the result is negative.

2.2 the PCEMNR micronucleus test: the result is negative.

2.3 the mouse sperm deformity test: the result is negative.

2.4 the mouse testis chromosomal aberration test: the result is negative.

3, rat 30d feeding trial: each test group animal growth is good in experimental period, every indexs such as body weight, food utilization, routine blood test, blood biochemistry, organ coefficient are all in range of normal value, and there are no significant between matched group difference (P＞0.05).Histopathologic examination is no abnormality seen also, illustrates that this sample does not produce the overt toxicity effect to the every observation index of rat.

Below by instantiation the present invention is done further narration, but this is not to be concrete qualification to the scope of the invention:

Example one

Hangzhoupro virus increases immunization therapy sexual dysfunction thing extraction example

1, it is as follows to take by weighing raw material:

Radix Panacis Quinquefolii 23 grams, Semen Strychni 1.5 grams, Folium Camelliae sinensis 2.3 grams, volt Siberian cocklebur 0.9 gram, Folium Bambusae 0.3 gram, Rhizoma Polygonati 5.5 grams, pollen of Semen Fagopyri Esculenti 3.7 grams, Radix Glycyrrhizae 5.1 grams, royal jelly powder 15.7 grams, Semen Cuscutae 0.4 gram, Fructus Corni 1.3 grams, Radix Polygoni Multiflori 9.8 grams, Herba Saussureae Involueratae 3 grams, Folium Mori 0.9 gram, Radix Puerariae 62.7 grams, forrest silkvine stem or root 1.5 grams, Pericarpium Citri Reticulatae Viride 46.3 grams, Hippocampus 46.3 grams, Rhizoma Dioscoreae 1.3 grams, Radix Salviae Miltiorrhizae 10.2 grams, Radix Notoginseng 3 grams, Lentinus Edodes 0.9 gram, Formica fusca 0.45 gram, Cordyceps 0.8 gram, Flos Caryophylli 15.4 grams, Cortex Moutan 0.9 gram, what leaf 0.4 gram, the Radix Astragali 32.6 grams, Cornu Cervi Pantotrichum 9.4 grams, thousand pins, ten thousand money grass, 15.4 grams, Rhizoma Corydalis Decumbentis 46.3 grams, Herba Agrimoniae 0.6 gram, the Pseudobulbus Bletillae (Rhizoma Bletillae) 0.2 gram, Rhizoma Alismatis 0.9 gram, Auricularia Sophorae 0.3 gram, Fructus Crataegi 0.6 gram, Herba Cistanches 48.6 grams, Fructus Lycii 6 grams, Herba Epimedii 57.4 grams, Rhizoma Chuanxiong 0.3 gram, Herb Gynostemmae Pentaphylli 0.4 gram, Ganoderma 10 grams, Radix Rehmanniae 1.3 grams, propolis 10 grams, Bulbus Allii 5 grams, Flos Lonicerae 10 grams, yeast 1 gram, lactobacillus 1 gram.

2, the processing step operation promptly gets extract of the present invention in the following order

A, raw material preliminary working

1) put into your low temperature sterilization of jacketed pan after Cornu Cervi Pantotrichum is pulverized, put into container with lid, add 65% pure grain, stir from making 95 degree high concentration Chinese liquor, make Pulveratum Cornu Cervi Pantotrichum slightly tide feel, closely put that it fully to be soaked in 4 hours standby.

2) with Bulbus Allii peeling, that the Fructus Crataegi enucleation is broken into the mud shape with common beater grinder is standby.

3) with surplus stock pre-freeze 8 hours under the condition below-30 ℃, be crushed to 60 order powder rapidly with common beater grinder.

4) with the whole mixing and stirring of above-mentioned all raw materials.

B, spray painting recovery

With the powder stock of mixing and stirring, joining one lentamente, to stir its rotating speed be 8r/min, spray in the culture tank of 5% normal saline mist vapour, in jar relatively a temperature be 45%, and constant temperature was cultivated 80 minutes for 30 ℃.

C, freeze film and low temperature superfine grinding

1) raw material of rapidly above-mentioned processes being crossed was put under-30 ℃ of conditions freezing 8 hours, took out and adopted the Universalpulverizer of topping up nitrogen to be ground into 600 order fine powders, reentered above-mentioned mist vapour sprayed tank and cultivated.

2) repeating above-mentioned spray painting recovers, freezes film, low temperature ultrafine grinding process three times.

D, supercritical Co ₂Extraction

1) extract solutes is respectively fluid Co ₂Its purity reach 99% and pure grain from making 95 degree high concentration Chinese liquor, its solute and raw material ratio are 10: 1.

2) extracting pressure is 35 ℃ of 35MPa separation temperatures, Co ₂Flow velocity is 25kg/h extraction time 5h, and the extraction product of winning obtain final extraction product with heating up through the secondary blood pressure lowering to separate, and first separating pressure is 10MPa, 38 ℃ of separation temperatures, Co ₂Flow velocity is 20kg/h extraction 2h, and second separating pressure is 40 ℃ of 7MPa ± 0.4MPa second separation temperatures, Co ₂Flow velocity is 15kg/h extraction 3h.

3) collect extract, will add pure grain through the high speed centrifugation device and separate with extract from making 95 degree high concentration Chinese liquor, the rotating speed of centrifugal device is 25000r/mim.

E, the multifunctional integrated lixiviate of medium constant temperature pressure rotation microwave

Will be through supercritical Co ₂The dusty raw materials of extraction adds the FQ538-II type medium constant temperature pressure rotation multifunctional integrated digester of microwave (referring to Fig. 9).

1) raw material being put in the extraction pot of 50 liter capacities, is the solvent lixiviate with pure grain from making 95 degree high concentration Chinese liquor, and solvent and raw material ratio are 10: 1.

2) open the digester speed of agitator 8r/mim that flats turn, extracting temperature is 30 ℃ of constant temperature, extracting pressure in jar is 1.2 atmospheric pressures, every two hours starts the 8kw supersonic generator voluntarily and handles 1 hour, and the excusing from death wave producer is the 60 seconds discontinuous dutys in 60 seconds gaps of work.

3) raw material gets lixiviating solution through one Multi Role Aircraft lixiviate 48 hours.

F, centrifugalize

With the centrifugalize of high speed centrifugation device, the centrifugal device rotating speed is that 3000r/min separates multi-functional lixiviating solution, wherein filtering residue combo, biotransformation and filtrate; Filtrate is carried out absorb-elute technologies such as ion exchange resin.

G, filtering residue combo

With the residue of centrifugal removal solution, add pollen of Semen Fagopyri Esculenti through ultra-fine pulverize at low temperature, Lentinus Edodes, Fructus Crataegi each 30%, the water of Mel 10%, yeast 1%, lactobacillus 1%, 8 multiple dose mixes to stir well and spares into reactor.

H, biotransformation and filtrate

1) under 29 ℃ of environment of constant temperature, carried out hermetically sealed biotransformation 48 hours through the material that the stirs biological respinse still of packing into, rotating speed 8r/mim of per 30 minutes mixs, venting in per 3 hours once, sealing continue to transform again, material is that fragrant bent flavor is dense can to carry out ferment filtrate.

2) adopt filter press, carry out the refining filtration of fermentation liquid with 1.5 ‰ ultrafiltration membranes earlier, wash adsorbing to take off behind ferment filtrate and the lixiviating solution mix homogeneously.

I, absorb-elute, to lixiviating solution, ferment filtrate in the following order with the mode absorb-elute that circulates.

1) adopting ion exchange resin that heavy metal lead, hydrargyrum, arsenic etc. are carried out selectivity ion exchange earlier removes.

2) employing is residual to organochlorine pesticide to the adsorbent resin that chlorinated organics has selective absorption, carries out selective absorption and removes.

3) the activated earth adsorbent carries out adsorbing and removing to the sensitization source.

J, nanometer micropore fine straining and concentrated

The employing aperture is that the micropore fine filtration technology of 120 nanometers is carried out the refining filtration of nanometer micropore to extracting solution, fermentation eluent through absorb-elute, through the hollow membrane pervaporation nanometer micropore is made with extra care filtrate simultaneously and concentrates.

K, vacuum freeze-drying

1) will add 1/5th distilled water through the lixiviate concentrated solution of nanometer micropore fine straining, put into vacuum freeze drier.

2) quick freezing under-45 ℃ of conditions subsequently is 10u in vacuum, and vapour pressure is the hydrone that freezes directly to be distilled overflow for steam, just can obtain being spongiform dry extract.

1, omnipotent pulverizing of topping up nitrogen and mixing

Vacuum freeze-drying is spongy extract goes into the Universalpulverizer of topping up nitrogen and be ground into fine powder more than 600 orders, promptly get extract of the present invention with lipid active pharmaceutical carrier mix homogeneously again.

Example two

Antiviral increases immunization therapy sexual dysfunction extract---the preparation example of lipid active pharmaceutical carrier.

(1), raw material is prepared and preliminary working:

1. get raw material: Folium Camelliae sinensis 230 grams, Folium Mori 90 grams, Herba Saussureae Involueratae 300 grams, Bulbus Allii 500 grams, Fructus Crataegi 60 grams, forrest silkvine stem or root 150 grams.

2. raw material preliminary working: with the Bulbus Allii peeling, it is standby that the Fructus Crataegi enucleation is broken into the mud shape with common beater grinder.

3. with surplus stock pre-freeze 8 hours under the condition below-3 ℃, be crushed to 60 order powder rapidly with common beater grinder.

4. with the whole mixing and stirring of above-mentioned all raw materials.

(2), the raw material spray painting is recovered:

With the powder stock of above-mentioned mixing and stirring, joining one lentamente, to stir its rotating speed be 8 rev/mins, sprays in the culture tank of 5% normal saline mist vapour relatively that humidity is 45%, and constant temperature was cultivated 80 minutes for 30 ℃.

(3), freeze film, low temperature superfine grinding:

1. rapidly the raw material of above-mentioned processes is put into taking-up in freezing 8 hours under-30 ℃ of conditions, adopted topping up nitrogen Universalpulverizer to be ground into 600 order fine powders, reenter aforementioned spray painting recovery technology.

2. repeat above-mentionedly, spray painting recovery, freeze film, low temperature ultrafine grinding process three times.

(4), supercritical Co ₂Extraction:

1. will insert supercritical Co through three finished raw materials of iterative process ₂In the extraction kettle of fluid extraction device, make itself and supercritical Co ₂Fluid fully contacts, dissolves, extracts, separates, and its process conditions are set as follows: extraction is pressed and is 35MPa, and extraction temperature is 35 ℃, Co ₂Fluid flow is 25kg/h, and the extraction time is 5h, adds pure grain in the extraction process from making 95 degree high concentration Chinese liquor, and addition is 10 times of the input material quantity.

2. collect extract, will add pure grain through the high speed centrifugation device and separate with extract from making 95 degree high concentration Chinese liquor, the rotating speed of centrifugal device is 25000r/min, and the gained article are lipid active pharmaceutical carrier of the present invention.(see figure 8)

Example three

Antiviral increases immunization therapy sexual dysfunction extract buccal tablet and makes example

1. the extract 70% for the treatment of excess syndrome example 1, the lipid active pharmaceutical carrier 20% for the treatment of excess syndrome example 2 stirs.

2. regulate and control 25 ℃ ± 2 ℃ of operate outside ambient temperatures, relative humidity is under 30% the condition, the duty of rotary tablet machine to be adjusted, and at first simulated pressure is fixed on 40KN, and the practical operation pressure in the production process is adjusted at 12KN.Adjust the filling thickness of tablet by the size of mould according to actual requirement then, fix on 13～15mm.

3. extract that stirs and lipid pharmaceutical carrier are added material bin, start rotary tablet machine, can produce the extract buccal tablet.

4. contain tablet form and can be olive-type, ball-type, shuttle type, round in any.

Example four

Antiviral increases immunization therapy sexual dysfunction extract soft capsule and makes example

(1) the extract lyophilized powder 25% for the treatment of excess syndrome example 1; The lipid active pharmaceutical carrier 25% for the treatment of excess syndrome example 2; And polyoxyethylene (40) monostearate (S-40) 50%.

(2) above-mentioned mixing of materials is mixed thoroughly with homogenizer, the packing of directly instiling gets final product.

Example five

Antiviral increases immunization therapy sexual dysfunction extract microcapsule formulation and makes example

1. the extract 25% for the treatment of excess syndrome example 1; The lipid active pharmaceutical carrier 30 as one kind % for the treatment of excess syndrome example 2, polyoxyethylene (40) monostearate (S-40) that adds 2 times of amounts carries out stirring and dissolving.

2. adopting the aperture is that the micropore fine filtration technology of 150 nanometers is made with extra care filtration to solution.

3. add hydroxy methocel 45% in the cleaner liquid, constantly stir.

4. cooling off the post crystallization body promptly gets microcapsule through ultra-fine freezing and pulverizing to 600 purpose of topping up nitrogen Universalpulverizer powder and claims molecular clathrate (Inclusion Compound) (electromicroscopic photograph is seen Fig. 7) again.

Example six

Antiviral increases immunization therapy sexual dysfunction thing nasal cavity administrated preparation and makes example

1. the extract 60 for the treatment of excess syndrome example 1 restrains, and Borneolum Syntheticum 20 grams mix through topping up nitrogen Universalpulverizer and are crushed to 180 purpose powder, by the packing of every dose 2.5 gram, suck use for nasal cavity through the vacuum quantitative racking machine.

2. the extract 1.4 for the treatment of excess syndrome example 1 gram, Borneolum Syntheticum or Mentholum 0.5 gram, hydroxy methocel 10 grams, distilled water 100ml, with described raw material through stirring abundant dissolving, the quantitative collunarium bottle of packing, every bottle of 2ml splashes into administration for nasal cavity, at every turn the amount of splashing into 0.1ml.

Claims (10)

1, a kind of antiviral increases immunization therapy sexual dysfunction thing extraction feed, it is characterized in that:

Radix Panacis Quinquefolii, Semen Strychni, Folium Camelliae sinensis, Fu Ling, Folium Bambusae, Rhizoma Polygonati, pollen of Semen Fagopyri Esculenti, Radix Glycyrrhizae, royal jelly powder, Semen Cuscutae, Fructus Corni, Radix Polygoni Multiflori, Herba Saussureae Involueratae, Folium Mori, Radix Puerariae, forrest silkvine stem or root, Pericarpium Citri Reticulatae Viride, Hippocampus, Rhizoma Dioscoreae, Radix Salviae Miltiorrhizae, Radix Notoginseng, Lentinus Edodes, Formica fusca, Cordyceps, Flos Caryophylli, Cortex Moutan, He Ye, the Radix Astragali, Cornu Cervi Pantotrichum, thousand pins, ten thousand money grass, Rhizoma Corydalis Decumbentis, Herba Agrimoniae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Alismatis, Auricularia Sophorae, Fructus Crataegi, Herba Cistanches, Fructus Lycii, Herba Epimedii, Rhizoma Chuanxiong, Herb Gynostemmae Pentaphylli, Ganoderma, Radix Rehmanniae, propolis, Bulbus Allii, Flos Lonicerae, yeast, lactobacillus.

2, according to the raw materials used prescription of the described extract of claim 1, it is characterized in that:

Radix Panacis Quinquefolii 23 grams, Semen Strychni 1.5 grams, Folium Camelliae sinensis 2.3 grams, volt Siberian cocklebur 0.9 gram, Folium Bambusae 0.3 gram, Rhizoma Polygonati 5.5 grams, pollen of Semen Fagopyri Esculenti 3.7 grams, Radix Glycyrrhizae 5.1 grams, royal jelly powder 1 5.7 grams, Semen Cuscutae 0.4 gram, Fructus Corni 1.3 grams, Radix Polygoni Multiflori 9.8 grams, Herba Saussureae Involueratae 3 grams, Folium Mori 0.9 gram, Radix Puerariae 62.7 grams, forrest silkvine stem or root 1.5 grams, Pericarpium Citri Reticulatae Viride 46.3 grams, Hippocampus 46.3 grams, Rhizoma Dioscoreae 1.3 grams, Radix Salviae Miltiorrhizae 10.2 grams, Radix Notoginseng 3 grams, Lentinus Edodes 0.9 gram, Formica fusca 0.45 gram, Cordyceps 0.8 gram, Flos Caryophylli 15.4 grams, Cortex Moutan 0.9 gram, what leaf 0.4 gram, the Radix Astragali 32.6 grams, Cornu Cervi Pantotrichum 9.4 grams, thousand pins, ten thousand money grass, 15.4 grams, Rhizoma Corydalis Decumbentis 46.3 grams, Herba Agrimoniae 0.6 gram, the Pseudobulbus Bletillae (Rhizoma Bletillae) 0.2 gram, Rhizoma Alismatis 0.9 gram, Auricularia Sophorae 0.3 gram, Fructus Crataegi 0.6 gram, Herba Cistanches 48.6 grams, Fructus Lycii 6 grams, Herba Epimedii 57.4 grams, Rhizoma Chuanxiong 0.3 gram, Herb Gynostemmae Pentaphylli 0.4 gram, Ganoderma 10 grams, Radix Rehmanniae 1.3 grams, propolis 10 grams, Bulbus Allii 5 grams, Flos Lonicerae 10 grams, yeast 1 gram, lactobacillus 1 gram.

3, a kind of antiviral increases the extraction and the preparation of immunization therapy sexual dysfunction thing, it is characterized in that technological process is raw material preliminary working, spray painting recovery, freezes film, the ultra-fine freezing and pulverizing of low temperature, supercritical Co ₂The multifunctional integrated lixiviate of extraction pharmaceutical carrier, medium constant temperature pressure rotation microwave, centrifugalize, filtering residue combo, biotransformation and consider liquid, absorb-elute, nanometer micropore fine straining and concentrate, vacuum freeze-drying, the omnipotent pulverizing of topping up nitrogen mix, be processed into the Types of Medicine preparation.

4, increase the extraction that the sexual dysfunction thing is controlled in immunity according to the described a kind of antiviral of claim 3, it is characterized in that specific embodiment is:

A, raw material preliminary working

1) Cornu Cervi Pantotrichum is put into the jacketed pan low temperature sterilization after pulverizing, and has put into the molten device of lid, adds 65% pure grain from making 95 degree high concentration Chinese liquor, stirs, and makes slightly tide sense of Pulveratum Cornu Cervi Pantotrichum, close putting 4 hours, and it is standby that it is fully soaked into.

2) with Bulbus Allii peeling, that the Fructus Crataegi enucleation is broken into the mud shape with common beater grinder is standby.

3) with surplus stock pre-freeze 8 hours under the condition below-3 ℃, be crushed to 60 order powder rapidly with common beater grinder.

4) with the whole mixing and stirring of above-mentioned all raw materials.

B, spray painting recovery

With the powder stock of mixing and stirring, joining one lentamente, to stir its rotating speed be 8 rev/mins, spray in the culture tank of 5% normal saline mist vapour, in jar relatively humidity be 45%, and constant temperature was cultivated 80 minutes for 30 ℃.

C, freeze film and low temperature superfine grinding

1) raw material of rapidly above-mentioned processes being crossed was put under-30 ℃ of conditions freezing 8 hours, took out, and adopted the Universalpulverizer of topping up nitrogen to be ground into the segmentation of 600 orders, reentered above-mentioned mist vapour sprayed tank and cultivated.

2) repeating above-mentioned spray painting recovers, freezes film, low temperature ultrafine grinding process three times.

D, supercritical Co ₂Extraction

1) extract solutes is respectively fluid Co ₂Its purity reach 99% and pure grain from making 95 degree high concentration Chinese liquor, its solute and raw material ratio are 10: 1.

2) extracting pressure is 35MPa, 35 ℃ of separation temperatures, Co ₂Flow velocity is the 25kg/h 5 hours time of extraction, and the extraction product of winning obtain final extraction product with heating up through the secondary blood pressure lowering to separate, and first separating pressure is 10MPa, 38 ℃ of separation temperatures, Co ₂Flow velocity is 20kg/h extraction 2 hours, and second separating pressure is 7MPa ± 0.4MPa, 40 ℃ of second separation temperatures, Co ₂Flow velocity is 15kg/h extraction 3 hours.

3) collect extract and will add pure grain through the high speed centrifugation device and spend the high concentration Chinese liquor and separate with extract from making 95, the rotating speed of centrifugal device is 25000r/min.

E, the multifunctional integrated lixiviate of medium constant temperature pressure rotation microwave

1) raw material being put in 50 liters of molten long-pending extraction pot, is the solvent lixiviate with pure grain from making 95 degree high concentration Chinese liquor, and solvent and raw material ratio are 10: 1.

2) open the digester speed of agitator 8r/min that flats turn, extracting temperature is 30 ℃ of constant temperature, extracting pressure in jar is 1.2 atmospheric pressures, every two hours starts the 8KW supersonic generator voluntarily and handles 1 hour, and the excusing from death wave producer is the 60 seconds discontinuous dutys in 60 seconds gaps of work.

3) raw material gets lixiviating solution through one Multi Role Aircraft lixiviate 48 hours.

F, centrifugalize

With the centrifugalize of high speed centrifugation device, the centrifugal device rotating speed is that 3000r/min separates multi-functional lixiviating solution, wherein filtering residue combo, biotransformation and filtrate, and filtrate is carried out absorb-elute technologies such as ion exchange resin.

G, filtering residue combo

With the residue of centrifugal removal solution, add pollen of Semen Fagopyri Esculenti through ultra-fine pulverize at low temperature, Lentinus Edodes, Fructus Crataegi each 30%, the water of Mel 10% and yeast 1% and lactobacillus 1%, 8 multiple doses stirs into reactor.

H, biotransformation and filtrate

1) under 29 ℃ of environment of constant temperature, carried out hermetically sealed biotransformation 48 hours through the material that the stirs biological respinse still of packing into, rotating speed 8r/min of per 30 minutes mixs, venting in per 3 hours once, sealing continue to transform again, material is that fragrant bent flavor is dense can to carry out ferment filtrate.

2) adopt filter press, select for use 1.5 ‰ ultrafiltration membranes to carry out the refining filtration of fermentation liquid, wash adsorbing to take off behind ferment filtrate and the lixiviating solution mix homogeneously.

I, absorb-elute encircle with mode in the following order lixiviating solution, ferment filtrate and to follow absorb-elute.

1) adopting ion exchange resin that heavy metal lead, hydrargyrum, arsenic etc. are carried out selectivity ion exchange earlier removes.

2) employing is residual to organochlorine pesticide to the resin that chlorinated organics has selective absorption, carries out selective absorption and removes.

3) the activated earth adsorbent carries out adsorbing and removing to the sensitization source.

J, nanometer micropore fine straining and concentrated

The employing aperture is that the micropore fine filtration technology of 120 nanometers is carried out the refining filtration of nanometer micropore to extracting solution, fermentation eluent through absorb-elute, through the hollow membrane pervaporation nanometer micropore is made with extra care filtrate simultaneously and concentrates.

K, vacuum freeze-drying

1) will add 1/5th distilled water through the lixiviate concentrated solution of nanometer micropore fine straining, put into vacuum freeze drier.

2) quick freezing under-45 ℃ of conditions subsequently is 10u in vacuum, and vapour pressure is the hydrone that freezes directly to be distilled overflow for steam, just can obtain being spongiform dry extract.

L, the omnipotent pulverizing of topping up nitrogen and mixing

Vacuum freeze-drying is spongy extract goes into the Universalpulverizer of topping up nitrogen and be ground into fine powder more than 600 orders, promptly get extract of the present invention with lipid active pharmaceutical carrier mix homogeneously again.

5, increase the extraction preparation that the sexual dysfunction thing is controlled in immunity as antiviral as described in claim 3 or 4, it is characterized in that described extract preparation is a buccal tablet.

6, increase the extraction preparation that the sexual dysfunction thing is controlled in immunity as antiviral as described in claim 3 or 4, it is characterized in that described contain tablet form be in olive-type, ball-type, shuttle type, the disk type any.

7, increase the extraction preparation that the sexual dysfunction thing is controlled in immunity as antiviral as described in claim 3 or 4, it is characterized in that described extract is a soft capsule.

8, increase the extraction preparation that the sexual dysfunction thing is controlled in immunity as antiviral as described in claim 3 or 4, it is characterized in that described microcapsule formulation.

9, increase the extraction preparation that the sexual dysfunction thing is controlled in immunity as antiviral as described in claim 3 or 4, it is characterized in that nasal cavity administrated preparation.

10, a kind of preparation claim 3 or 4 described lipid active pharmaceutical carriers is characterized in that:

A, supercritical Co ₂Fluid extraction is pressed and is 35MPa, and extraction temperature is 35 ℃, Co ₂Fluid flow is to be 5h the 25kg/h extraction time.

B, extractant are respectively fluid Co ₂Its purity reach 99% and pure grain from making 95 degree high concentration Chinese liquor, its solvent is 10 times of raw material addition.

C, collection extract will add pure grain through the high speed centrifugation device and separate with extract from making 95 degree high concentration Chinese liquor, and the rotating speed of centrifugal device is 25000r/mim.

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