CN1513833A - Method of preparing high quality compound amino acid by hydrolyzing protein - Google Patents
Method of preparing high quality compound amino acid by hydrolyzing protein Download PDFInfo
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- CN1513833A CN1513833A CNA02134924XA CN02134924A CN1513833A CN 1513833 A CN1513833 A CN 1513833A CN A02134924X A CNA02134924X A CN A02134924XA CN 02134924 A CN02134924 A CN 02134924A CN 1513833 A CN1513833 A CN 1513833A
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Abstract
A process for preparing high-quality compound amino acid from hydroprotein features that the activated carbon adsorption column and '717' anionic resin exchange column are used for separating the hydrolytic liquid of protein to obtain single kind of amino acid and greatly decrease the content of ash, resulting in high-purity crystal of compound amino acid.
Description
The present invention relates to the method for compound amino-acid that constitutes by 18 kinds of single amino acids with protein hydrolyzate preparation, especially the method for preparing about the regulation ratio of 8 kinds of indispensable amino acids in 18 seed amino acids according to FAO/WHO.
At present, 17 seed amino acids that generate in the protein hydrolyzate, because aspartic acid and L-glutamic acid are acidic amino acids, its iso-electric point is a pH value 2.77 to 3.22, needs the pH value of its solution is brought up to 6 when above, add alkaline matter; Arginine, Methionin and Histidine are basic aminoacidss, and its iso-electric point to pH value 0.76, and the pH value of their solution will be reduced to PH6.0 the time, add acidic substance at pH value 7.6.Salt content in the compounded amino acid product of producing like this raises, and can't realize the low high-quality compounded amino acid product of ash.In addition, because proline(Pro) is water-soluble extremely strong, can not from the aqueous solution, crystallization separate out.Serine solubleness is also very big, makes kilnitamin non-crystallizable separating out in the aqueous solution, therefore, can't prepare crystallization compounded amino acid product at all.Compounded amino acid product in the market, very dark for color, the ash content of coal reaches the product of 3.5% spraying drying preparation.This product can only be used as food raw material because the ash content of coal is too big, can not be used as medical material.And to prepare pharmaceutical compounded amino acid starting material, and can only buy various single amino acid products to prepare voluntarily, the cost of the compounded amino acid product of Sheng Chaning like this is more than the expensive twice of present method.
The present invention uses chromatograph exclusion chromatographies such as active carbon adsorption column and 717 resin anion(R.A) exchange columns, acidic amino acids such as aspartic acid and L-glutamic acid, and arginine, Methionin, basic aminoacidss such as Histidine separation and Extraction respectively come out, reduced significantly because of acidic amino acid and basic aminoacids influence the ash content of coal in the compounded amino acid product, thereby improved quality product, enabled to reach the pharmaceutical quality standard.
The present invention uses chromatograph exclusion chromatographies such as active carbon adsorption column and 717 resin anion(R.A) exchange columns, the big proline(Pro) of solubleness in water and Serine respectively separation and Extraction come out, make pure product, thereby kilnitamin is separated out with their mixed crystal mode crystallization, obtain highly purified kilnitamin crystallisate, make originally the aminoacids complex of can't crystallization separating out become the compounded amino acid product that easy crystallization is separated out.
Chromatograph exclusion chromatographies such as active carbon adsorption column that the present invention uses and 717 resin anion(R.A) exchange columns are a kind of fixed separable programmings, and any proteolysis transforms the amino acid mixing liquid that generates, and is not subjected to the influence of above-mentioned separable programming.Because range protein is bigger to the content amplitude of a certain or a few seed amino acids, so, by the kind of conversion protein hydrolysate, can prepare complete various aminoacids content according to a certain specific proportion requirement.
8 kinds of essential amino acids content ratios in the compounded amino acid product of the present invention's preparation are to be as the criterion with the ratio that FAO/WHO stipulates.See the following form:
| The amino acid title | Different bright | Bright | Rely | Egg+Guang | Phenylpropyl alcohol+junket | Soviet Union | Figured silk fabrics | Look | Add up to |
| The regulation ratio | ??4％ | ??7％ | ??5.5％ | ??3.5％ | ??6％ | ??4％ | ??5％ | ??1％ | ??36％ |
The present invention adopts the multiple proteins hydrolysis to prepare the mixing prod of multiple different 8 kinds of essential amino acids content, and 8 kinds of indispensable amino acid mixing prod that meet the aforementioned proportion requirement for preparation provide condition.Some compounded amino acid product pupa albumens are that raw material is made in the market.7 kinds of essential amino acids content analyzing pupa albumen in theory all meet the table requirement, add tryptophane in addition after, meet the 8 seed amino acid regulation proportion requirement of FAO/WHO, but that practice is gone up is really not so about aminoacids complex.When producing, often in decolorization, phenylalanine and tyrosine are all taken off, remained little.The major part of leucine, Isoleucine and Xie Ansuan also is adsorbed, because the discoloring agent gac all has bigger adsorptive power to these amino acid.So compounded amino acid product in the market no matter from about aspect analyses such as 8 kinds of essential amino acids content ratios, the ash content of coal, color and lusters, all is low-quality products.The advantage of the inventive method is can prepare high-quality compounded amino acid product from above three aspects, to satisfy the demand of social production and people life.
The present invention will now be further detailed embodiment:
100 gram degreasing silkworm chrysalises are placed 800 milliliters of round-bottomed flasks, add 300 milliliters of 6N hydrochloric acid solns, 110 ℃ of hydrolysis 18 hours heat up, collect and transform the amino acid solution that generates, the water that adds four times of hydrolyzed solution volumes dilutes, and regulating PH with liquefied ammonia again is 9.0, allows the activated carbon column of its 4.0cm * 20.0cm that flows through, flow through the again 717 resin anion(R.A) exchange columns of 4.0cm * 20.0cm, isolate aspartic acid 9.2 grams, L-glutamic acid 9.8 grams, arginine 4.7 grams, Methionin 6.9 grams, Histidine 2.4 grams, proline 3 .2 gram, Serine 3.8 grams, kilnitamin crystal 4 4.6 grams add up to 84.6 grams.The total yield of aminoacids complex is 84.6%.8 kinds of indispensable amino acid amounts are Isoleucines 4.02%, leucine 6.84%, Methionin 7.2%, methionine(Met) 3.48%, phenylalanine and tyrosine 8.8%, Threonine 3.98%, 4.4%, seven kind of indispensable amino acid of Xie Ansuan adds up to 38.72%, add 1% tryptophane again, overall proportion reaches 39.72%, considerably beyond 36% proportion requirement of FAO/WHO about 8 kinds of indispensable amino acid regulations, has realized high-quality compounded amino acid product.
Claims (8)
1, protein hydrolysate prepares the method for compound amino-acid that 18 kinds of single amino acids are formed, and it is characterized in that proteolysis is transformed the 17 seed amino acid mixed solutions that generate separates the mixed crystallization that obtains aspartic acid and L-glutamic acid selectively with chromatograph exclusion chromatographies such as active carbon adsorption column and 717 resin anion(R.A) exchange columns; Separate and obtain the single composition kinds of basic aminoacids such as arginine, Methionin, Histidine; Separate the single composition kind that obtains proline(Pro), Serine and Threonine; Other neutral amino acidss all are prepared as the mixed crystallization product.17 kinds of prepared single amino acids add in addition the tryptophane by 1% ratio, make the compounded amino acid product that is made of 18 kinds of single amino acids of the ratio standard that meets 8 kinds of indispensable amino acids that FAO/WHO stipulates.
2, aminoacids complex preparation method according to claim 1, it is characterized in that cutin albumen such as protein raw material behave to be sent out, pig hair, or people such as pig blood meal, gelatin, gelatine, silk, silkworm chrysalis, plant oil cake and the livestock protein raw material that can not directly utilize.
3, aminoacids complex preparation method according to claim 1, its feature comprises activated carbon column, chromatograph separating devices such as 717 resin anion(R.A) exchange columns and 732 cation exchange resin columns.
4, aminoacids complex preparation method according to claim 1, its feature gac, 717 resin anion(R.A)s and 732 resin cation (R.C.)s are separating medium.
5, aminoacids complex preparation method according to claim 1, it is characterized in that 8 kinds of tryptophanes in the indispensable amino acid for adding preparation, other 7 kinds of indispensable amino acids are to require formulated by the kilnitamin that different proteins prepares in proportion according to the analytical test result.
6, aminoacids complex preparation method according to claim 1, it is characterized in that thoroughly isolating 2 kinds of acidic amino acids from 17 seed amino acid mixed solutions, the mixed crystal of remaining kilnitamin behind 3 kinds of basic aminoacidss and proline(Pro), the Serine is as the raw material of preparation.
7, aminoacids complex preparation method according to claim 1 is characterized in that acidic amino acid and basic aminoacids are neutralized to mutually below the PH6.0, and the mixed crystallization that is obtained is used in the preparation aminoacids complex as non-essential amino acid and gone.
8, aminoacids complex preparation method according to claim 1 is characterized in that adding as required isolated proline(Pro) and Serine in the compounded amino acid product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA02134924XA CN1513833A (en) | 2002-10-10 | 2002-10-10 | Method of preparing high quality compound amino acid by hydrolyzing protein |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA02134924XA CN1513833A (en) | 2002-10-10 | 2002-10-10 | Method of preparing high quality compound amino acid by hydrolyzing protein |
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| CN1513833A true CN1513833A (en) | 2004-07-21 |
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| CNA02134924XA Pending CN1513833A (en) | 2002-10-10 | 2002-10-10 | Method of preparing high quality compound amino acid by hydrolyzing protein |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1880940B (en) * | 2005-06-17 | 2011-11-30 | 马永健 | Instrument for separating and measuring tryptophan |
| CN115088847A (en) * | 2022-07-13 | 2022-09-23 | 呼伦贝尔市林海森林经营管理有限公司 | Method for extracting multiple amino acids from birch juice |
-
2002
- 2002-10-10 CN CNA02134924XA patent/CN1513833A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1880940B (en) * | 2005-06-17 | 2011-11-30 | 马永健 | Instrument for separating and measuring tryptophan |
| CN115088847A (en) * | 2022-07-13 | 2022-09-23 | 呼伦贝尔市林海森林经营管理有限公司 | Method for extracting multiple amino acids from birch juice |
| CN115088847B (en) * | 2022-07-13 | 2023-12-22 | 呼伦贝尔市林海森林经营管理有限公司 | Method for extracting multiple amino acids from birch juice |
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