CN1086965A - Process for hydrolyzing protein - Google Patents
Process for hydrolyzing protein Download PDFInfo
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- CN1086965A CN1086965A CN92101937A CN92101937A CN1086965A CN 1086965 A CN1086965 A CN 1086965A CN 92101937 A CN92101937 A CN 92101937A CN 92101937 A CN92101937 A CN 92101937A CN 1086965 A CN1086965 A CN 1086965A
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Abstract
The present invention is a kind of process for hydrolyzing protein that is raw material with chicken, duck slag or other animal flesh slag.This technology be earlier with raw material and hydrochloric acid with 1: the ratio mixing of (0.9-1.5), soak (100-105 ℃ of (75-95 ℃, 18-12 hours), hydrolysis through temperature, refluxed 4 hours), get the concentrate of pH5-6.5 behind filtration and the enrichment process, after the refrigeration desalination and filtering, make 31 seed amino acids again.Adopt technology of the present invention also can obtain feed product and can prepare delicious monosodium glutamate soy sauce.
Description
The present invention is a kind of process for hydrolyzing protein that is raw material with chicken, duck slag or other animal flesh slag.
By recognizing in No. 85102242 application documents of Chinese patent " aminosal separation method of amino-acids ", animal and plant protein hydrolysate is divided into A, B two parts by GH-15 charcoal post, the B part flows out through the charcoal post, A partly stays on the charcoal post, water, acid solution, ethanol etc. are made into release agent, amino acid on the charcoal post escaped to be become single or two seed amino acids mix frees liquid, allows its cation exchange column of flowing through again, segments out the amino acid of single variety.Wherein, be protein hydrolysising material with the pig blood meal, because the acidolysis reaction in this method is too quickly, destroyed amino acid whose kind, so after the chromatography, only obtain 9 kinds of single amino acids, this is a defective one.Make protein hydrolysising material with the pig blood meal, production cost is higher, and this is a defective two.In addition, also there is the single defective of product category.
The objective of the invention is provides a kind of process for hydrolyzing protein of improved acid-hydrolysis method at above-mentioned defective.This technology can not only turn waste into wealth, and can obtain the amino acid of numerous kinds, and does not produce the pernicious gas contaminated air in the whole production technological process.
The object of the present invention is achieved like this: become animal protein with hydrochloric acid hydrolysis.Operating process be earlier with animal flesh slag and hydrochloric acid with 1: the raw material behind the ratio mixing of (0.9～1.5), through temperature soak, after the hydrolysis, filtration, enrichment process, get the concentrate of PH5～6.5, after again this concentrate being passed through refrigeration desalination, filtration, make aminosal, wherein, it is 75～95 ℃ that temperature is soaked temperature, 18～12 hours time, hydrolysis temperature is 100～105 ℃, and the time is to reflux 4 hours.
The present invention learns from other's strong points to offset one's weaknesses because the acid hydrolyzation technology of prior art is improved, so following advantage is arranged:
One; overcome the disadvantage of destroying the amino acid kind in the prior art, adopted the present invention can obtain following 31 seed amino acids: alanine; proline; valine; leucine; isoleucine; arginine; histidine; tyrosine; phenylalanine; lysine; phosphoserine; taurine; phosphoethanolamine; Rui's propylhomoserin; serine; glycine; citrulling; cystine; methionine; cystathionie; monoethanolamine; oxylysine; prosperous propylhomoserin; 1-Methyl histidine; 3-Methyl histidine; anserine; carnosine; hydroxyproline; asparagine and glutamic acid.This Duos 19.2% than the amino acid (26 kinds) in " hundred Lan Shi " chickens' extract oral liquid that sells well all over the world of Britain's product, and the amino acid kind of reporting than Chinese patent 85102242 has increased nearly 4 times.This kind result's reason is that technological operation of the present invention has overcome the amino acid whose principle of the too quickly destruction of acidolysis reaction, make its acidolysis condition steadily, relax, safety causes.
Its two, adopt aminosal that technology of the present invention obtains through long-term (30 days) successive administration test, do not see overt toxicity, and hemopoietic system had wholesome effect on the contrary.Therefore, with its preparation oral liquid human body being had no adverse reaction, is safe.
They are three years old, utilize offal (as the offal duck slag after the production " nourishing teal essence ") to produce aminosal, not only reached the purpose that turns waste into wealth, and the production cost of producing oral liquid is reduced greatly, productivity ratio nourished the smart 40-50% of raising of teal originally, taste is more tasty, and nutrition is abundanter.
Its four, the comprehensive utilization ratio height both can get three kinds of products such as feed, aminosal and preparation monosodium glutamate soy sauce, did not have waste residue again and produced.
Its five, adopt to reclaim the hydrochloric acid operation among the present invention after, both can reduce production costs, do not pollute the environment again.
Accompanying drawing is a process chart of the present invention.
The invention will be further described below in conjunction with embodiment and accompanying drawing thereof.
Referring to accompanying drawing, technological process of the present invention is that raw material duck slag (or chicken slag or other animal flesh slag) is soaked in acid (hydrochloric acid solution) lower temperature earlier, and temperature is soaked temperature 75-95 ℃, and the time is 18-12 hour, through 100-105 ℃, 4 hours hydrolysis of backflow, get hydrolyzate.
Then hydrolyzate is filtered: impurity layer (containing filter residue) through neutralization (neutrality) and≤drying of 90 ℃ of temperature, make feed product, filtrate is the liquid after water layer is filtered, reclaim hydrochloric acid after, concentrated and at 60-65 at 100-105 ℃ of lower normal pressure ℃ lower reduced pressure concentration becomes concentrate.
After again concentrate being used 10%NaOH liquid neutralization (PH5-6.5), there be 0--5 ℃ of lower refrigeration desalination to spend the night, separate out the NaCl crystallization. After the filtration, filtrate is the aminosal product; Filter cake NaCl crystal can be made into the monosodium glutamate soy sauce.
Embodiment one:
Get 10 kilograms of duck slags or chicken slag and add hydrochloric acid solution 15l mixing in porcelain enamel barrel, temperature is controlled at 80-90 ℃ of water proof insulation 12 hours then, hydrolysis is in the pasty state gradually to make duck, change in the enamel reactor that has agitator and reflux condensation mode apparatus through siphon, speed of agitator is controlled at 30 rev/mins, reaction temperature is at 100-105 ℃, hydrolysis 4 hours.When becoming brown liquid, hydrolyzate stops to stir.The logical running water of the interlayer of jar cools off, and is chilled to room temperature.Can observe hydrolyzate from the materail tube visor is homogeneous state, slowly tells the sour water layer.Filter cake after the filtration (impurity layer) is collected together with unhydrolysed bits, neutralization, dry back used as fodder, and filtrate then flows into enrichment process.
When concentrating, carry out normal pressure earlier and concentrate, temperature is controlled at 100-105 ℃, with 102 ℃ better, reclaim hydrochloric acid solution.It is concentrated to reduce pressure again, and temperature is controlled at 60-65 ℃, with 60 ℃ better.Be equipped with two on the material pipe between concentration tank and the vavuum pump and absorb basin.Wherein be close in the basin of concentration tank and fill 25% NaOH solution, another basin is blow-down tank (being safety can).When concentrating, decompression stops to concentrate when being the thickness state.
Concentrate is chilled to room temperature, emits feed liquid, the NaOH with 10% transfers to PH5-6.5, spends the night in 0--5 ℃ of refrigeration, separates out the NaCl crystallization.After the filtration, filtrate is aminosal, and filter cake NaCl crystal can add the monosodium glutamate soy sauce that is made into special fragrant delicate flavour in the common soy sauce.
Embodiment two:
The product of the technological process of present embodiment, operating process and made is all with embodiment one.Below listed table one provide three kinds of schemes, tables two that four kinds of schemes are provided, all best with scheme two effects.
Table one
The present invention can adopt sulfuric acid, hydrochloric acid, nitric acid, acetic acid, phosphoric acid, citric acid, malic acid and fumaric acid etc. to carry out acid hydrolysis, is the best with hydrochloric acid as hydrolytic reagent just, and other kind is lower as amino acid kind instability and the yield that hydrolytic reagent makes.
Claims (5)
1, a kind of process for hydrolyzing protein, become animal protein with hydrochloric acid hydrolysis, it is characterized in that earlier animal flesh slag and hydrochloric acid with 1: the raw material behind the ratio mixing (0.9-1.5), through temperature soak, after the hydrolysis, filtration, enrichment process, get the concentrate of PH5-6.5, after again this concentrate being passed through refrigeration desalination, filtration, make aminosal, wherein, it is 75-95 ℃ that temperature is soaked temperature, time 18-12 hour, hydrolysis temperature was 100-105 ℃, and the time is to reflux 4 hours.
2, process for hydrolyzing protein according to claim 1 is characterized in that the hydrolyzate that described raw material makes behind hydrolyzing process, be chilled to room temperature and filter, and its filtrate flow is to enrichment process, and its impurity layer becomes feed product by neutralization and dry (≤90 ℃).
3, process for hydrolyzing protein according to claim 2 is characterized in that described filtrate normal pressure under 100-105 ℃ of temperature concentrates and reclaim hydrochloric acid solution, is condensed into the concentrate of PH5-6.5 again with 60-65 ℃ of temperature decompression.
4, according to claim 1 or 3 described process for hydrolyzing protein, it is characterized in that described concentrate,, spend the night with 0--5 ℃ of temperature refrigeration again that separate out the NaCl crystallization, filtrate is aminosal earlier with the neutralization of 10%NaOH liquid.
5, process for hydrolyzing protein according to claim 4 is characterized in that described NaCl crystallization is made into the monosodium glutamate soy sauce.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92101937A CN1086965A (en) | 1992-03-24 | 1992-03-24 | Process for hydrolyzing protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92101937A CN1086965A (en) | 1992-03-24 | 1992-03-24 | Process for hydrolyzing protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1086965A true CN1086965A (en) | 1994-05-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN92101937A Pending CN1086965A (en) | 1992-03-24 | 1992-03-24 | Process for hydrolyzing protein |
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| Country | Link |
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| CN (1) | CN1086965A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069502C (en) * | 1997-09-12 | 2001-08-15 | 薛连钦 | Meat lipid residue and its making method |
| CN102067939A (en) * | 2009-11-19 | 2011-05-25 | 德清县嘉润骨胶有限公司 | Process for preparing feed protein powder |
| CN102283308A (en) * | 2011-06-20 | 2011-12-21 | 北京工商大学 | Snail protein hydrolysis liquid and preparation method thereof |
| CN110419618A (en) * | 2019-07-25 | 2019-11-08 | 李猷 | A kind of animal albumen powder and preparation method thereof rich in carnosine |
-
1992
- 1992-03-24 CN CN92101937A patent/CN1086965A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069502C (en) * | 1997-09-12 | 2001-08-15 | 薛连钦 | Meat lipid residue and its making method |
| CN102067939A (en) * | 2009-11-19 | 2011-05-25 | 德清县嘉润骨胶有限公司 | Process for preparing feed protein powder |
| CN102283308A (en) * | 2011-06-20 | 2011-12-21 | 北京工商大学 | Snail protein hydrolysis liquid and preparation method thereof |
| CN110419618A (en) * | 2019-07-25 | 2019-11-08 | 李猷 | A kind of animal albumen powder and preparation method thereof rich in carnosine |
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