A kind of Bulbus Allii protein zymolyte series of products and uses thereof
Technical field
The present invention relates to the development and use field of vegetable protein, refer in particular to a kind of Bulbus Allii protein zymolyte series of products that contain 2~10 peptide active polypeptide and uses thereof.
Technical background
A large amount of medical researches show, that Bulbus Allii has is antibiotic, antiviral, blood pressure lowering and health cares such as blood fat, atherosclerosis, antitumor and adjusting body immunity.
The main approach that utilizes of Bulbus Allii mainly comprises up to now: (1) utilizes dehydration techniques such as hot air drying, lyophilization, preparation Bulbus Allii powder or sheet, and serve as to prepare burden to produce series of products with it; (2) extract Oleum Bulbus Allii, and serve as to prepare burden to produce series of products with it; (3) extract garlicin, and serve as to prepare burden to produce series of products with it; (4) extract alliin, and serve as to prepare burden to produce series of products with it.As Chinese invention patent " manufacturing process of no foul smell garlic powder " (CN86100334B) and " preparation method of Chinese garlic oil pill " (CN1048884C), invention disclosed patent " continuous feeding process for extracting garlic oil by supercritical CO 2 " (publication number 1376770), " garlicin and garlic oil cyclodextrine derivative clathrate and preparation method thereof " (publication number 1448129) and " method of extraction alliin from Bulbus Allii " (publication number 1273969).
More than all do not relate to the proteic problem of utilizing of Bulbus Allii about development and use and the patent of Bulbus Allii, Bulbus Allii is after isolating Oleum Bulbus Allii, about protein more than 20% is arranged in the remaining residue, and therefore waste is very serious, and the technology that has not yet to see relevant residue intensive processing occurs.
Summary of the invention
The present invention is to provide the proteic zymolysis technique of a kind of Bulbus Allii, make the enzymolysis solution series of products that comprise 2~10 peptide bioactive peptides, have bringing high blood pressure down, improve immunity, function such as defying age, antiviral, especially wherein blood pressure lowering peptide, have very strong ACE and suppress active, being also referred to as ACE and suppressing bioactive peptide, is some special peptide matters that are formed by connecting in sequence by specific aminoacid.This bioactive peptide reduces hyperpietic's blood pressure by the inhibitory action to tonin (being called for short ACE), is the healthy goods raw material of a kind of blood pressure lowering safely and effectively.
Another object of the present invention provides a kind of preparation method of above-mentioned Bulbus Allii protein zymolyte.
Above-mentioned purpose of the present invention realizes by following technological means.
A kind of Bulbus Allii protein enzymatic hydrolyzate, it is characterized in that component in the dry of this enzymolysis solution, weight percent content are as follows: polypeptide compounds content is 70~80%, and polysaccharide compound content is 5~15%, and surplus is an aminoacid.
The technology that Bulbus Allii proteolysis legal system is equipped with bioactive peptide is as follows:
Removing the proteinic residue of Bulbus Allii that comprises that obtains after Oleum Bulbus Allii or the garlicin with Bulbus Allii is raw material, adopts enzymatic hydrolysis by adding protease, makes the protein zymolyte that is rich in bioactive peptide.The present invention has adopted the method for single enzymolysis and double-enzyme hydrolysis, can protein digestion in garlic residue such as the dry powder residue be become the active polypeptide and the amino acids material of 2~10 peptides under the condition of gentleness.The preparation method of this enzymolysis solution comprises: the Bulbus Allii dry powder residue is broken → and enzymolysis → enzyme denaturing → centrifugal → filtration → ultrafiltration → zymolyte.
Being operating as of enzymatic hydrolysis: with garlic residue with water dissolution after, add protease according to the concentration of 5~15AU/kg, be 5~9 at pH, temperature is under 30 ℃~60 ℃ the condition, to carry out enzymolysis 0.5~5 hour.
Employed protease comprises: endo proteases such as trypsin, flavor protease, compound protease, pepsin, alkaline protease, neutral protease, and the compound enzyme that is made of endo protease.
Zymolyte can be made different liquors and powder goods by following process.
This zymolyte can be mixed with oral liquid through concentrating; Also can directly take then through concentrated and dry, or and mixed with excipients, make powder, capsule etc., can also make an addition in other food as food additive in addition.
This zymolyte can be used to reduce hyperpietic's blood pressure by the inhibitory action to tonin (ACE), has the immunity of raising, blood lipid regulation, antidotal function simultaneously.
The specific embodiment
Below in conjunction with embodiment the present invention is further elaborated:
Embodiment 1, single enzymolysis (1)
It is broken that Bulbus Allii is got Oleum Bulbus Allii, garlicin or alliin Bulbus Allii dry powder residue afterwards, take by weighing 100g Bulbus Allii dry powder residue, add dissolved in distilled water, ratio according to 5AU/kg adds flavor protease, transfer pH to 6 with sodium hydroxide and hydrochloric acid, in thermostat water bath, be heated to 40 ℃ of following enzymolysis 3h of enzyme operative temperature.In the enzymolysis process, constantly adjust pH and make its value remain on steady state value.After enzymolysis finishes, heat 100 ℃ and boil and rise 15min, stop enzymolysis, the ACE that carries out zymolyte suppresses active detection about 78%.
Embodiment 2, single enzymolysis (2)
Take by weighing 100g Bulbus Allii dry powder residue, add dissolved in distilled water, according to the ratio adding neutral protease of 15AU/kg, transfer pH to 7 with sodium hydroxide and hydrochloric acid, keeping temperature is 30 ℃ of following enzymolysis 2h.In the enzymolysis process, constantly adjust pH and make its value remain on steady state value.After enzymolysis finished, ebuillition of heated 15min stopped enzymolysis, and the ACE that carries out zymolyte suppresses active and detects about 82%.
Embodiment 3, double-enzyme hydrolysis
Take by weighing 100g Bulbus Allii dry powder residue, add dissolved in distilled water, the ratio adding compound protease Protamex according to 8AU/kg transfers pH to 8 with sodium hydroxide and hydrochloric acid, and keeping temperature is 50 ℃ of following enzymolysis 3h.With the post-heating enzyme denaturing.Further the ratio according to 10AU/kg adds alkaline protease, transfers pH to 9, and keeping temperature is 60 ℃ of following enzymolysis 2h.After enzymolysis finishes, regulate pH to 7, carry out enzyme denaturing, the ACE that carries out zymolyte suppresses active and detects about 85%.
Embodiment 4, the capsular preparation of Bulbus Allii polypeptide powder
The Bulbus Allii residue protein enzymatic hydrolyzate of above-mentioned preparation is carried out centrifugalize remove water-insoluble impurity, further filter and make with extra care, remove macromolecular material, prepare the Bulbus Allii polypeptide powder by spray drying then by ultrafiltration, through canned, make Bulbus Allii polypeptide powder capsule.
Embodiment 5, the preparation of Bulbus Allii polypeptide oral liquor
Bulbus Allii residue enzymolysis solution is carried out centrifugalize, filters refining, hyperfiltration treatment, concentrate 20 times, carry out proportioning in following ratio: Bulbus Allii residue enzymolysis concentrated solution: Mel: potassium sorbate=100: 10: 0.06, through the sterilization bottling, make the Bulbus Allii polypeptide oral liquor.