CN1511941A - Closed pipeline type photosynthetic reactor - Google Patents

Closed pipeline type photosynthetic reactor Download PDF

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Publication number
CN1511941A
CN1511941A CNA021497915A CN02149791A CN1511941A CN 1511941 A CN1511941 A CN 1511941A CN A021497915 A CNA021497915 A CN A021497915A CN 02149791 A CN02149791 A CN 02149791A CN 1511941 A CN1511941 A CN 1511941A
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China
Prior art keywords
reactor
algae liquid
algae
pipe
pipeline
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Granted
Application number
CNA021497915A
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Chinese (zh)
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CN1208451C (en
Inventor
向文洲
何慧
林坚士
董俊德
何妙娟
吴伯堂
曾呈奎
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South China Sea Institute of Oceanology of CAS
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South China Sea Institute of Oceanology of CAS
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Priority to CN 02149791 priority Critical patent/CN1208451C/en
Publication of CN1511941A publication Critical patent/CN1511941A/en
Application granted granted Critical
Publication of CN1208451C publication Critical patent/CN1208451C/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate

Abstract

The closed pipeline type photosynthetic reactor is used for culturing microalgae or other photosynthetic onganism. It has structure including light accepting reaction pipeline, algae liquid storing bottle and reactor driving system with windbag pump. The algae liquid storing bottle, the sucking pipe of the light accepting reaction pipeline, the windbag pump, the reflux pipe of the light accepting reaction pipeline are connected successively to form one algae liquid circulating loop, with side ventilating pipe being connected to the sucking pipe. The present invention has low cost and high microalgae yield and may be used in mass production.

Description

A kind of closed conduct formula photosynthetic reactor
Technical field
The present invention relates to a kind of reactor that is used to cultivate little algae or other photosynthetic organism.
Background technology
Photosynthetic reactor (photobioreactor) claims bioreactor again, flows in closed container or stirring by algae liquid, improves photosynthetic efficiency and output, is used for the little algae of high-efficient culture or other photosynthetic organism.And open cultivation pool commonly used is aborning at present cultivated facility, adopts open Da Chi (at the bottom of the formation ponds such as earth of cement, PVC plate, compacting or pool wall) to cultivate little algae.Bioreactor has the following advantages because the closure of system is compared with open cultivation pool: can avoid or alleviate the pollution of other algae or harmful organisms, keep single and plant or the sociales cultivation conditions, guarantee output and quality; The efficiency of light energy utilization of reactor significantly improves than open cultivation pool, and can adopt the high-density culture pattern, increases substantially output, reduces the cost of gathering; The algae all cells can evenly be subjected to light in the reactor, avoids stirring in the open pond insufficient and superficial cell supersaturation that cause is subjected to light and following confluent monolayer cells to be subjected to the insufficient situation of light, the raising photosynthetic efficiency; Can control culture condition effectively, the required target product of directed production; Can avoid culture (as poisonous algae, transgenosis algae, isotopic labeling algae etc.) that environment is produced unfavorable or damaging effect.
The deficiency that bioreactor exists mainly shows as: the closure of system causes oxygen and dissolved oxygen to accumulate in a large number, growth metabolism is produced suppress or destruction; Make stirring of algae liquid or mobile power system produce coup injury to algae; Controlled condition system and material cost height, thereby limited the amplification and the commercial application of its scale.
The bioreactor that a lot of standards are arranged at present; can be divided into duct type from the shape of photoresponse container; pot type; board-like etc.; duct type bioreactor wherein; because can be by pipeline freely extending and fold spatially; increase substantially light-receiving area and reaction volume; thereby carry out the little algae of large-scale production than other standard is easier; but still exist power system (as impeller pump; surge pump etc.) frustule is easily caused shortcomings such as coup injury; and the airlift reactor of comparatively generally acknowledging at present; though the lifting force that can utilize gas to rise in pipeline to produce drives the circulation of algae liquid; reduced the coup injury of power system to algae liquid; but because its pipeline reactor can not infinitely extend; the oxygen that can not effectively get rid of in the tubing system to be accumulated, thereby limited it and cultivated the expansion of scale.In the airlift reactor culturing process; because a large amount of accumulation of oxygen form blocking-up algae liquid mobile gas column and the oxygen bubbles that adheres to above the tube wall easily; and influence the normal reflux cycle of algae liquid and evenly be subjected to light; particularly under high light, culture is brought damage and destruction; simultaneously; a large amount of accumulations of dissolved oxygen also can produce inhibition, damage even destruction to frustule in the pipeline, and growth efficiency also has much room for improvement.
Summary of the invention
The objective of the invention is to propose a kind ofly can increase substantially little algae output, accomplish scale production, and the low closed conduct formula photosynthetic reactor new texture of cost.
The structure of this closed conduct formula photosynthetic reactor, comprise and be subjected to the photoresponse pipeline, algae liquid liquid storage bottle and reactor power system, it is characterized in that with the power system of bellows pump as reactor, the said photoresponse pipeline that is subjected to divides two portions, tail pipe and return line, the input terminus of its tail pipe is connected with algae liquid liquid storage bottle, output terminal is connected with the fluid inlet of bellows pump, the liquid outlet of bellows pump is connected with the input terminus of return line, the output terminal of return line is connected to algae liquid liquid storage bottle, forms algae liquid circulation loop, is connected with the ventilation side pipe on said tail pipe.
This photosynthetic reactor is work like this: after bellows pump starts, along with the stretching back and forth and the contraction of windbag, algae liquid enters in the windbag through upstream line from liquid storage bottle, is extruded from windbag then, flow back to liquid storage bottle through reflux line, make the circulation continuously in reactor of algae liquid.
For excessive dissolved oxygen and oxygen in the timely eliminating pipeline, at the side pipe of ventilating near connection on the tail pipe, so that air admission or interpolation carbonic acid gas, wherein during irradiation, the effect that enters the side pipe of ventilating with air and carbon dioxide mix is good.Can in pipe reactor, insert a Y-pipe, the side pipe of ventilating on the termination of Y-pipe, two ends are communicated with reaction tubes in addition, make air, external source CO 2Or the mixture of the two enters in the reaction tubes additional CO equably 2, increase the gaseous interchange in the reaction tubes, reduce dissolved oxygen, get rid of oxygen bubbles.
Described ventilation side pipe can be the tubule of a caliber below 2mm, also can connect the tubule of a caliber below 2mm at the end of ventilation side pipe and constitute.Said tubule generally adopts kapillary.
The present invention has following technique effect: bellows pump does not have coup injury to frustule; The energy consumption of bellows pump is very low; Suck air continuously or add carbonic acid gas by the ventilation side pipe, the harm that reduces a large amount of accumulation of oxygen in the pipe reactor and bring, can not form sticking wall oxygen bubbles and gas column simultaneously at tube wall, form on the contrary with algae liquid mobile bubble, increase the gas-liquid exchange in the pipeline, and make frustule evenly be subjected to light, reduce dissolved oxygen concentration, reduce light and suppress, thereby increase substantially output; The present invention can select pipeline material cheaply for use, and the power cost of running bellows pump is lower, is beneficial to commercial application.
Description of drawings
Fig. 1 is the structural representation of closed conduct formula photosynthetic reactor of the present invention.
Embodiment
Be described in further detail below in conjunction with the CONSTRUCTED SPECIFICATION of accompanying drawing the embodiment of the invention:
As shown in Figure 1, this photosynthetic reactor is with the power system of bellows pump 1 as photosynthetic reactor, the plastic conduit that with diameter is 6 millimeters is as the photoresponse pipeline, comprise tail pipe 21 and return line 22, the output terminal of the input terminus of tail pipe 21 and return line 22 is inserted among the algae liquid liquid storage bottle 8, tail pipe 21 output terminals are connected with the fluid inlet 3 of bellows pump, the liquid outlet 4 of bellows pump is connected with the input terminus of return line, form algae liquid circulation loop, tail pipe 21 close algae liquid liquid storage bottle positions reaching 10 meters connect a side shoot glass capillary 6 as the ventilation side pipes by Y-pipe 5.Liquid storage bottle adds 1 liter of spissated spirulina algae liquid 7, and algae liquid density is 3.2 grams per liters, starts bellows pump, makes the circulation cultivation continuously in reactor of algae liquid.Culture temperature 25-32 ℃, light intensity 5000-50,000Lux, cultured continuously three days, average unit volume output reaches 0.8 grams per liter/sky

Claims (4)

1. closed conduct formula photosynthetic reactor, comprise and be subjected to the photoresponse pipeline, algae liquid liquid storage bottle and reactor power system, it is characterized in that with the power system of bellows pump (1) as reactor, the said photoresponse pipeline that is subjected to divides two portions, tail pipe (21) and return line (22), the input terminus of its tail pipe is connected with algae liquid liquid storage bottle (8), output terminal is connected with the fluid inlet of bellows pump, the liquid outlet of bellows pump is connected with the input terminus of return line, the output terminal of return line is connected to algae liquid liquid storage bottle (8), forms algae liquid circulation loop, is connected with the ventilation side pipe on said tail pipe (21).
2. closed conduct formula photosynthetic reactor according to claim 1 is characterized in that the end of described ventilation side pipe is connected to the tubule of a caliber below 2mm.
3. closed conduct formula photosynthetic reactor according to claim 1 is characterized in that described ventilation side pipe is the tubule of caliber below 2mm.
4. according to claim 2 or 3 described closed conduct formula photosynthetic reactor, it is characterized in that described tubule is a kapillary.
CN 02149791 2002-12-31 2002-12-31 Closed pipeline type photosynthetic reactor Expired - Lifetime CN1208451C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 02149791 CN1208451C (en) 2002-12-31 2002-12-31 Closed pipeline type photosynthetic reactor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 02149791 CN1208451C (en) 2002-12-31 2002-12-31 Closed pipeline type photosynthetic reactor

Publications (2)

Publication Number Publication Date
CN1511941A true CN1511941A (en) 2004-07-14
CN1208451C CN1208451C (en) 2005-06-29

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CN 02149791 Expired - Lifetime CN1208451C (en) 2002-12-31 2002-12-31 Closed pipeline type photosynthetic reactor

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1317379C (en) * 2004-07-16 2007-05-23 中国科学院海洋研究所 Pipeline photobioreactor for scale culture of microalgae
CN102408982A (en) * 2010-09-26 2012-04-11 新奥科技发展有限公司 Photobioreactor
CN105647826A (en) * 2015-11-11 2016-06-08 深圳市绿得宝保健食品有限公司 Culture method and device of photosynthetic microorganism

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1317379C (en) * 2004-07-16 2007-05-23 中国科学院海洋研究所 Pipeline photobioreactor for scale culture of microalgae
CN102408982A (en) * 2010-09-26 2012-04-11 新奥科技发展有限公司 Photobioreactor
CN102408982B (en) * 2010-09-26 2015-04-01 新奥科技发展有限公司 Photobioreactor
CN105647826A (en) * 2015-11-11 2016-06-08 深圳市绿得宝保健食品有限公司 Culture method and device of photosynthetic microorganism
CN105647826B (en) * 2015-11-11 2019-05-10 深圳市绿得宝保健食品有限公司 A kind of cultural method and device of photosynthetic microorganism

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Publication number Publication date
CN1208451C (en) 2005-06-29

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Granted publication date: 20050629