CN1508266A - Biochip detecting system - Google Patents
Biochip detecting system Download PDFInfo
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- CN1508266A CN1508266A CNA021564272A CN02156427A CN1508266A CN 1508266 A CN1508266 A CN 1508266A CN A021564272 A CNA021564272 A CN A021564272A CN 02156427 A CN02156427 A CN 02156427A CN 1508266 A CN1508266 A CN 1508266A
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Abstract
The invention is a biochip detecting system, composed of semiconductor cooling CCD camera, high-efficiency object lens, X-Y 2-D table, bar code detect-read head, filament lamp, camera bellows, and computer. Use the bar code detect-read head to scan the biochip with chemical luminescence label, make the computer resister by identifying sample, then place the sample on the X-Y planar table, adjust the planar table to make the sample at the center of the object lens, close the door of the camera obscura to make the sample in the camera obscura, image the chemical luminescence signal on the CCD camera, and set the CCD parameters by software in the computer, like time of exposure, resolution, etc, to start collecting the chemical luminescence image.
Description
Affiliated technical field
The present invention relates to a kind of biological chips detection system.
Background technology
Gene chip and protein chip are the biochips that is used for detecting simultaneously the expression level of several genes or multiple protein; it will become topmost molecular diagnosis means in this century; it is of many uses; to not only be used for medical diagnosis, also will be used for fields such as health care, drug screening, environment protection, agricultural, public security, solution civil dispute and prevention epidemic prevention.
The instrument that is used for biochip test in the market mainly is divided into laser scanning type and CCD type, U.S. Axon wherein, Packand and Affymetrix company have released the laser scanning type gene chip tester, this kind detector generally all has two above laser apparatus, obtains the image of entire chip by spot scan.And the high level error in order to prevent that high-density from bringing, high-density gene chip all adopts hybridizes equivalent simultaneously but is with different fluorescently-labeled samples and with reference to product; U.S. Alpha lnnotech company has released CCD type high-density gene chip detector, this detector need adopt Polaroid to the portion gene chip with 16 high precision CCD, obtain the image of entire chip then by splicing, this detector comprises that also a wide spectrum light source excites the fluorescent mark on the chip.More than two kinds of instruments mainly be detection at high-density gene chip, the plant and instrument action is complicated, cost is very expensive.
Along with protein chip and gene chip move towards clinical from scientific research gradually, the market share of low density gene chip and protein chip will be more and more higher also.In order to catch up with the needs of clinical application, a kind of simple and easy biochip dedicated test system is extremely urgent.
Summary of the invention
Technology of the present invention is dealt with problems and is: a detection system is provided, and it can detect the chemiluminescence signal on low density gene chip and the protein chip directly expeditiously exactly, and cost is low, and is economical and practical.
Technical solution of the present invention is: biological chips detection system, its characteristics are: by semi-conductor cooling CCD camera, the high-level efficiency object lens, X-Y two dimension platform, bar code inspection read head, incandescent light, camera bellows, computer is formed, the chemiluminescent biochip that is marked with that needs are detected scans on bar code inspection read head earlier, computer is registered with the identification sample, again sample is put on the X-Y two dimension platform, adjust X-Y two dimension platform, make sample be in the object lens center, the camera bellows door is closed, sample is in the camera bellows, chemiluminescence signal on the sample is caught by object lens simultaneously and is imaged on the CCD camera 1, by executive software the CCD parameter is set on computers, as the time shutter, resolving power etc. just can begin to gather the chemoluminescence image.
The present invention is applicable to chemiluminescence signal and the detection by quantitative X-ray film signal that detects on gene chip, the protein chip, its principle of work is: after print a series of biological chemistry of process or the medical treatment, on the mark chemiluminescent labeling, the power of chemiluminescence signal has just been reacted the content of each gene in sample, catch chemiluminescence signal by semi-conductor cooling CCD camera, after picture processing and image analysis, can accurately obtain the information of each spot of chip, comprise the size, optical density value, mean value of spot etc., so that further analyze.
The present invention's advantage compared with prior art is:
(1) the CCD camera of the present invention's use is semi-conductor cooling, science zero level camera, and its cooling temperature can arrive-30 ℃, and dark current is 0.01 electronics/pixel/second, can detect very faint luminous signal, and the time shutter of requirement is the longest by 60 minutes.The dark current of general imaging system is all in that 10 electronics/pixels/more than second, its noise of time exposure floods signal, can't distinguish noise and signal;
(2) adopt special-purpose large-numerical aperture object lens, phosphor collection efficient is very high;
(3) adopt bar-code reader to discern sample, clear being convenient to read;
(4) software system function of Pei Beiing is powerful, and the output result of spreadsheet can directly supply medical analysis.
Description of drawings
Fig. 1 forms synoptic diagram for the structure of detection system of the present invention;
Fig. 2 is software workflow figure of the present invention.
Embodiment
As shown in Figure 1, the present invention is by 12 semi-conductor cooling CCD cameras 1, high-level efficiency object lens 2, X-Y two dimension platform 3, bar code inspection read head 4, incandescent light 5, camera bellows 6, computer is formed, the chemiluminescent biochip that is marked with that needs are detected scans on bar code inspection read head 4 earlier, computer is registered with the identification sample, again sample is put on the X-Y two dimension platform 3, adjust X-Y two dimension platform 3, make sample be in object lens 2 centers, the door of camera bellows 6 is closed, sample is in the camera bellows, by executive software the CCD parameter is set on computers, as the time shutter, resolving power etc. just can begin to gather the chemoluminescence image.Chemiluminescence signal on the sample is caught by object lens 2 and is imaged on the CCD camera 1, again by after computer process picture processing and the image analysis, accurately obtain the information of each spot of chip, comprise the size, optical density value, mean value of spot etc., so that further analyze.
The focal length of above-mentioned high-level efficiency object lens 2 is 25mm, and numerical aperture is 0.95.
As shown in Figure 2, software flow of the present invention is as follows: open semi-conductor cooling CCD camera 1, and its working parameter is set, comprise time shutter and sensitivity or resolving power, being marked with chemiluminescent biochip is being placed on the high-level efficiency object lens 2 after the scanning on the bar code inspection read head 4 earlier, the signal of being caught by high-level efficiency object lens 2 by computer control CCD camera is imaged on the CCD camera 1, by image acquisition with after handling, carry out the spot location, spot calculates, comprise spot size, optical density value etc., make the electrical form histogram by computer at last, carry out medical analysis.
Claims (3)
1, biological chips detection system, it is characterized in that: by semi-conductor cooling CCD camera, the high-level efficiency object lens, X-Y two dimension platform, bar code inspection read head, incandescent light, camera bellows, computer is formed, the chemiluminescent biochip that is marked with that needs are detected scans on bar code inspection read head earlier, computer is registered with the identification sample, again sample is put on the X-Y two dimension platform, adjust X-Y two dimension platform, make sample be in the object lens center, the camera bellows door is closed, sample is in the camera bellows, chemiluminescence signal on the sample is caught by object lens simultaneously and is imaged on the half wire body cooling CCD camera, by executive software the CCD parameter is set on computers, gathers the chemoluminescence image.
2, biological chips detection system according to claim 1 is characterized in that: above-mentioned semi-conductor cooling CCD camera is 12.
3, biological chips detection system according to claim 1 is characterized in that: the focal length of above-mentioned high-level efficiency object lens is 25mm, and numerical aperture is 0.95.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02156427 CN1223683C (en) | 2002-12-16 | 2002-12-16 | Biochip detecting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02156427 CN1223683C (en) | 2002-12-16 | 2002-12-16 | Biochip detecting system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1508266A true CN1508266A (en) | 2004-06-30 |
| CN1223683C CN1223683C (en) | 2005-10-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02156427 Expired - Fee Related CN1223683C (en) | 2002-12-16 | 2002-12-16 | Biochip detecting system |
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| CN (1) | CN1223683C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926238A (en) * | 2013-01-14 | 2014-07-16 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Portable multipurpose chemiluminescence imager |
| CN103983576A (en) * | 2014-05-28 | 2014-08-13 | 上海勤翔科学仪器有限公司 | Full-automatic chemiluminiscent imaging device |
| CN106248578A (en) * | 2016-08-24 | 2016-12-21 | 中国科学院光电技术研究所 | A kind of integrated form protein chip scanner |
| CN107238716A (en) * | 2017-06-05 | 2017-10-10 | 江苏睿玻生物科技有限公司 | A kind of luminous detection work station of microfluid biochip |
| CN109917274A (en) * | 2019-03-27 | 2019-06-21 | 深圳市安思科电子科技有限公司 | A kind of stable type detection device with regulatory function for chip |
| CN110180811A (en) * | 2019-05-07 | 2019-08-30 | 王海山 | A kind of chip detecting equipment that the clarity with dehumidification function is high |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101936904A (en) * | 2010-08-31 | 2011-01-05 | 上海交通大学 | Portable fluorescence tomography detection system |
-
2002
- 2002-12-16 CN CN 02156427 patent/CN1223683C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926238A (en) * | 2013-01-14 | 2014-07-16 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Portable multipurpose chemiluminescence imager |
| CN103983576A (en) * | 2014-05-28 | 2014-08-13 | 上海勤翔科学仪器有限公司 | Full-automatic chemiluminiscent imaging device |
| CN106248578A (en) * | 2016-08-24 | 2016-12-21 | 中国科学院光电技术研究所 | A kind of integrated form protein chip scanner |
| CN107238716A (en) * | 2017-06-05 | 2017-10-10 | 江苏睿玻生物科技有限公司 | A kind of luminous detection work station of microfluid biochip |
| CN109917274A (en) * | 2019-03-27 | 2019-06-21 | 深圳市安思科电子科技有限公司 | A kind of stable type detection device with regulatory function for chip |
| CN110180811A (en) * | 2019-05-07 | 2019-08-30 | 王海山 | A kind of chip detecting equipment that the clarity with dehumidification function is high |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1223683C (en) | 2005-10-19 |
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