CN1493204A - Manufacturing method of polypeptide calf milk powder substitute - Google Patents
Manufacturing method of polypeptide calf milk powder substitute Download PDFInfo
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- CN1493204A CN1493204A CNA031325661A CN03132566A CN1493204A CN 1493204 A CN1493204 A CN 1493204A CN A031325661 A CNA031325661 A CN A031325661A CN 03132566 A CN03132566 A CN 03132566A CN 1493204 A CN1493204 A CN 1493204A
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Abstract
A polypeptide powder as the substitute of milk powder for calf is prepared through proportioning raw materials, preparing the aqueous solutions of soybean protein, defatted soybean powder and compound proteinase, adding them to protein emulsion, low-temp emylizing, adding beta-cyclodextrin, stirring, adding the aqueous solution of minerals, whey powder and methionine, stirring, concentrating, adding vitamines and oil, stirring, homogenizing and spray drying. It has high absorptivity.
Description
Technical field: the present invention relates to a kind of manufacture method for the edible powered milk substitute of calf.
Background technology: after the calf birth, physique a little less than, digestive system is sound as yet.In order to satisfy its quick growth, the needs of building up resistance, traditional feeding method is can only take food in early days colostrum and normal breast (fresh milk).Because colostrum has following function: 1, nutritious, easily digestion can replace intestines wall mucous membrane, stops bacterium to invade blood; 2, colostrum contains lysozyme and immunoglobulin (Ig), can kill multiple germ, thereby improves the calf Abwehrkraft des Koepers; 3, first lactic acid degree is higher, can make gastric juice become acid, suppresses the breeding of harmful bacterium; 4, colostrum can promote a large amount of digestive ferments of stomachial secretion, and the stomach and intestine function is formed as early as possible, can make calf get rid of meconium as early as possible again.The colostrum so new-born calve must be taken food helps growing of calf like this.Normal breast (fresh milk), be that the best source of nutrition of calf is (present, the fresh milk of feeding is the tradition feed method of China calf), it can provide comprehensive nutritive materials for growing of calf, and the contained non-lactoprotein of other solid feed during this period calf be difficult to absorb, thereby influence quick growth of calf and required nutriment, because when calf digests and assimilates system and does not perfect as yet, the non-newborn feed of feeding too early can influence growing of calf all one's life, cause from now on production and the decline of population quality, the underproduction, in addition dead, bring massive losses to aquaculture.But people know that fresh milk is again human best nutritional product, and commodity value is high, so guaranteeing more fresh milk supply dairy products market, create under the prerequisite of large economy benefit more, should reduce the calf rearing cost, satisfy the absorption of calf full nutrition and the needs of growth fast again.But also be not fit to the edible non-lactoprotein of calf and the milk replacer of high-absorbility in the market more.
Summary of the invention:, the invention provides the manufacture method of a peptide species calf meal for solving the problem of the milk replacer that lacks edible non-lactoprotein of calf and high-absorbility in the market.Manufacture method of the present invention is carried out as follows: one, take by weighing raw material by following weight ratio: soybean protein isolate 80-220, defatted soy flour 150-350, compound protease 0.125-0.48, water soluble vitamin 0.5-1.2, mineral matter 75-96, grease and fat soluble vitamin 205-226, whey powder 250-300, beta-schardinger dextrin-45-70, methionine 2-10; Two, make protein emulsion: soybean protein isolate and defatted soy flour are mixed with insatiable hunger Heshui solution; Three, make polypeptide protein liquid: compound protease is made insatiable hunger Heshui solution add then in the protein emulsion, place down at 35-40 ℃ and carried out enzyme hydrolysis in 1-2 hour, be warming up to 65-70 ℃ and be incubated 25-35 minute then; Four, make the polypeptide protein emulsion: in above-mentioned polypeptide protein liquid, add beta-schardinger dextrin-and under 40-60 ℃, mixed 25-30 minute; Five, make mixed emulsion: mineral matter is made insatiable hunger Heshui solution together join in the polypeptide protein emulsion with whey powder and methionine then, under 40-60 ℃, mixed 15-25 minute then; Six, make concentrated emulsion: mixed emulsion 135-140 ℃ of following instantaneous sterilization, and then concentrate is formed the concentrated emulsion of solid content at 45-50%; Seven, water soluble vitamin is mixed with the aqueous solution that insatiable hunger closes and together join in the concentrated emulsion, puddles at normal temperatures then, homogeneous 15-25 minute with grease and fat soluble vitamin; Eight, the last spray-dried finished product of making.Described water soluble vitamin is mixed by the raw material of following ratio of weight and number: VB1 is that 0.008-0.0085, VB2 are that 0.01-0.014, VB3 are that 0.016-0.018, VB11 are that 0.06-0.07, VB12 are 0.0001-0.0002 and aureomycin 0.5-1.Described grease and fat soluble vitamin are mixed by the raw material of following ratio of weight and number: animal or plant grease 160-170, soybean lecithin 45-55, VA0.02-0.03, VD0.002-0.004, VE0.0001-0.0003 and antioxidant 0.1-0.5.Described antioxidant is TBHQ.Described mineral matter is mixed by the raw material of following ratio of weight and number: MgSO
4H
2O is 4-6, FeSO
4H
2O is 0.3-0.4, MnSO
4H
2O is 0.1-0.2, ZnSO
4H
2O is 0.12-0.13, CuSO
45H
2O is that 0.04-0.05, KI are 0.0007-0.0009, CoCl
26H
2O is 0.0004-0.0006, Na
2SeO
3For 0.0007-0.0008, KCl are 11-14, NaCO
3H
2O is 12-13, H
3PO
4Be 23-27, CaCl
2Be 25-35.The product that the present invention produced is to have high-tech last word.It is to utilize the cold-adapted enzyme engineering that plant animal protein is hydrolyzed, thereby the mixture that is rich in multiple peptide that obtains, add saturated and unrighted acid then, naturally occurring emulsifying agent, multivitamin, multiple material such as trace element through sterilization, homogeneous, concentrate, modernized processing technology such as spray-drying makes, and is rich in calf grow required protein (with polypeptide form), amino acid, aliphatic acid, lactose, lecithin, calcium, phosphorus, vitamin and immune factor.The characteristics of the product polypeptide calf meal that the present invention produces: 1, have the nutrient absorption character of polypeptide products, solved the difficult problem that calf is difficult to absorb non-lactoprotein always; 2, can be slower because of the generation of calf pepsin, and influence the problems such as low digestibility that plant animal protein replaces lactoprotein; 3, reasonably combined saturated fatty acid, unrighted acid absorption rate after the emulsification embedding improves greatly; 4, be rich in the basic substance----lecithin of life; 5, substitute fresh milk fully, indivedual indexs are higher than fresh milk; 6, compared with similar products, thereby because the existence of polypeptide improves the absorptivity of protein, shorten feeding floor every; 7, high in technological content, technology is leading, and is leading domestic.
The specific embodiment: the production method of the polypeptide calf meal of present embodiment is carried out as follows: one, take by weighing raw material by the listed standard of table 1; Two, the defatted soy flour of 160 kilograms of 200 kilograms soybean separation protein bletillas is dissolved in the warm water about ten times 40 ℃ and mixes (concentration of substrate is below 10%), the pH value of adjusting the albumen mixed emulsion with the NaOH of 1mol/L or HCL solution is 6-7 simultaneously, soaks behind the stable protein emulsion of formation in 30 minutes stand-by.Because the temperature of this moment and the most suitable compound protease of pH value are to the hydrolysis of protein, low temperature hydrolysis can well be preserved amino acid whose nutritive value simultaneously; Three, with compound protease, be dissolved in 50 times the 30-40 ℃ of water, fully mixed dissolution is stand-by after half an hour, and then, the enzyme solutions that will prepare adds in the protein emulsion and mixed 1-2 hour, fully hydrolysis, degree of hydrolysis reaches more than 40%.Selecting compound protease is exactly in order to shorten the enzyme hydrolysis time, to improve degree of hydrolysis, and alleviate the bitter taste that produces behind the proteolysis.Protein in the last hydrolysate is hydrolyzed into relative molecular weight greatly about 1000-6000D, and chain length is the polypeptide protein liquid of 2-10 amino acid residue.In hydrolytic process, to constantly adjust the pH value of protein emulsion with NaOH or HCl, remain between the 6-7, thereby guarantee carrying out smoothly of hydrolysis.When hydrolysis reaches balance, temperature is risen to more than 70 ℃, keep enzyme being carried out Passivation Treatment in 30 minutes, form stable polypeptide protein liquid.Four, add 52.5 kilograms of beta-schardinger dextrin-s on this basis, fully mix forming the polypeptide protein emulsion in 30 minutes.This process is to utilize beta-schardinger dextrin-with hydrophobic amino acid embedding complexing in the polypeptide.Because in hydrolytic process, along with the continuous increase of polypeptide, when peptide molecular weight is lower than 6000D, can produce a large amount of exposures of hydrophobic amino acid, and the hydrophobic amino acid that exposes has bitter taste, thereby influence the product taste.Therefore, solve the problem of polypeptide bitter taste with the embedding complexing of beta-schardinger dextrin-.Below each operation be exactly preparation to polypeptide powered milk substitute nutritional labeling.Five, used preceding 10 minutes, mineral matter is added in the normal-temperature water, mix stand-by, for preventing that crystallization from taking place, puddle once more before the use, at first 5 kilograms of 280 kilograms of the aqueous solution, the whey powders, the methionine that prepare are added 60 ℃ and contain in the polypeptide protein emulsion of beta-schardinger dextrin-, mixes 20 minutes stable mixed emulsions of formation.Six, mixed emulsion is carried out the superhigh temperature instantaneous sterilization under 135 ℃ temperature, concentrate and form the concentrated emulsion of solid content at 45-50%.Seven, add in the normal-temperature water water soluble vitamin such as VB1, VB2, VB3, VB11, VB12 and aureomycin stand-by behind the mixed dissolution.With the water soluble vitamin for preparing and grease and fat soluble vitamin add puddle in the concentrated emulsion after 20 minutes high-pressure homogeneous, for spray-drying ready.Add the purpose of water soluble vitamin and grease and fat soluble vitamin this moment, be the destruction that prevents in high temperature sterilization and concentration process the multivitamin nutritional labeling, also can avoid high temperature to make soybean lecithin generation Maillard reaction simultaneously, influence product color and nutrient.In addition, add the grease that contains the naturally occurring emulsifying agent soybean lecithin before homogeneous, it is more even that oil-water mixture is mixed, and avoids the profit layering, influences product quality.At last, be 140-180 ℃ at EAT exactly, when being 80-85 ℃, temperature of outgoing air carries out spray-drying, form polypeptide powered milk substitute finished product.Moisture is controlled at below 6%, packs after the cooling, finish whole production processes.In packaging process, must control the health of utensils such as personnel and packing material well, in order to avoid bacterium exceeds standard.
Table one: the various raw material requirements of product per ton
| Material name | Content | Addition | |
| Protein emulsion | Protein isolate | Albumen 〉=90% | ????200Kg |
| Defatted soy flour | Albumen 〉=50% | ????160Kg | |
| Enzyme solutions | Compound protease | 100,000 IU | ????0.48Kg |
| Water soluble vitamin | ????VB1 | ?????????98％ | ????0.0082Kg |
| ????VB2 | ?????????98％ | ????0.0133Kg | |
| ????VB3 | ?????????98％ | ????0.0173Kg | |
| ????VB11 | ?????????98％ | ????0.065Kg | |
| ????VB12 | ?????????1％ | ????0.0001Kg | |
| Aureomycin | ?????????15％ | ????0.5Kg | |
| Mineral matter | ????MgSO 4.H 2O | ????Mg???16.1％ | ????5Kg |
| ????FeSO 4.H 2O | ????Fe???30％ | ????0.385Kg | |
| ????MnSO 4.H 2O | ????Mn???31.8％ | ????0.13Kg | |
| ????ZnSO 4.H 2O | ????Zn???36.1％ | ????0.127Kg | |
| ????CuSO 4.5H 2O | ????Cu???25％ | ????0.046Kg | |
| ????KI | ????I????75.7％ | ????0.0008Kg | |
| ????CoCl 2.6H 2O | ????Co???24.3％ | ????O.0005Kg | |
| ????Na 2SeO 3 | ????Se???44.7％ | ????0.00078Kg | |
| ????KCl | ????K????52.1％ | ????13Kg | |
| ????NaCO 3H 2O | ????Na???37％ | ????12.5Kg | |
| ????H 3PO 4 | ????P????31.6％ | ????25Kg | |
| ????CaCl 2 | ????Ca???36.1％ | ????30Kg | |
| Grease and fat soluble vitamin | Animal and plant fat | ?????????97％ | ????165Kg |
| Soybean lecithin | ?????????98％ | ????50Kg | |
| ????VA | 500,000 IU/g | ????0.024Kg | |
| ????VD | 500,000 IU/g | ????0.0028Kg | |
| ????VE | 500,000 IU/g | ????0.00013Kg | |
| Antioxidant (TBHQ) | ????99％ | ????0.2Kg | |
| Whey powder | Lactose 70-80% albumen 6-8% | ????280Kg | |
| Beta-schardinger dextrin- | ????95％ | ????52.5Kg | |
| Methionine | ????98％ | ????5Kg | |
| Add up to | ????1000Kg | ||
Table two: polypeptide calf meal nutritional labeling (seeing Table 1)
100 gram polypeptide calf meal nutrient composition contents
| Nutritional labeling | Standard value is not less than |
| Dry (gram) | ????94 |
| Protein (gram) | ????26 |
| Fat (gram) | ????20 |
| Lactose (gram) | ????20 |
| Ca (gram) | ????1.1 |
| P (gram) | ????0.8 |
| Mg (milligram) | ????80 |
| Fe (milligram) | ????11.5 |
| Mn (milligram) | ????4.6 |
| Zn (milligram) | ????4.6 |
| Cu (milligram) | ????1.15 |
| I (milligram) | ????0.058 |
| Co (milligram) | ????0.012 |
| Se (milligram) | ????0.035 |
| K (milligram) | ????650 |
| Cl (milligram) | ????250 |
| Na (milligram) | ????460 |
| Lysine (milligram) | ????3000 |
| Methionine (milligram) | ????900 |
| Vitamin A (IU) | ????1200 |
| Vitamin D (IU) | ????140 |
| Vitamin E (IU) | ????6.5 |
| Vitamin B1 (milligram) | ????0.8 |
| Vitamin B2 (milligram) | ????1.3 |
| Vitamin B3 (milligram) | ????1.7 |
| Pantothenic acid B11 (milligram) | ????0.065 |
| Folic acid B12 (milligram) | ????0.01 |
Table three: the nutritional labeling of fresh milk and polypeptide powered milk substitute emulsion is (seeing Table 2) relatively
100 gram fresh milk and polypeptide powered milk substitute emulsion nutritional labelings
| Nutritional labeling | Fresh milk | Polypeptide powered milk substitute emulsion |
| Dry (gram) | ????13.20 | ????14.25 |
| Protein (gram) | ????3.40 | ????4.2 |
| Fat (gram) | ????3.10 | ????3.15 |
| Lactose (gram) | ????3.00 | ????3.15 |
| Ca (milligram) | ????125 | ????165 |
| P (milligram) | ????95 | ????120 |
| Mg (milligram) | ????13 | ????12 |
| Fe (milligram) | ????0.05 | ????1.7 |
| Mn (milligram) | ????0.004 | ????0.69 |
| Zn (milligram) | ????0.39 | ????0.69 |
| Cu (milligram) | ????0.1 | ????0.17 |
| I (milligram) | ????-- | ????0.0087 |
| Co (milligram) | ????-- | ????0.0018 |
| Se (milligram) | ????1.5 | ????0.0053 |
| K (milligram) | ????156.7 | ????97.5 |
| Cl (milligram) | ????103 | ????37.5 |
| Na (milligram) | ????50.5 | ????69 |
| Vitamin A (IU) | ????150 | ????180 |
| Vitamin D (IU) | ????25 | ????21 |
| Vitamin E (IU) | ????0.5 | ????0.98 |
| Vitamin B1 (milligram) | ????0.05 | ????0.12 |
| Vitamin B2 (milligram) | ????0.14 | ????0.2 |
| Pantothenic acid B3 (milligram) | ????0.22 | ????0.26 |
| Folic acid B11 (milligram) | ????-- | ????0.075 |
| Cobalamin (milligram) | ????-- | ????0.0015 |
| Lysine (milligram) | ????250 | ????450 |
| Methionine (milligram) | ????130 | ????150 |
Annotate: polypeptide powered milk substitute: water=15: 85 (W/W)
Claims (5)
1, the manufacture method of polypeptide calf meal is characterized in that it carries out as follows: one, take by weighing raw material by following weight ratio: soybean protein isolate 80-220, defatted soy flour 150-350, compound protease 0.125-0.48, water soluble vitamin 0.5-1.2, mineral matter 75-96, grease and fat soluble vitamin 205-226, whey powder 250-300, beta-schardinger dextrin-45-70, methionine 2-10; Two, make protein emulsion: soybean protein isolate and defatted soy flour are mixed with insatiable hunger Heshui solution; Three, make polypeptide protein liquid: compound protease is made insatiable hunger Heshui solution add then in the protein emulsion, place down at 35-40 ℃ and carried out enzyme hydrolysis in 1-2 hour, be warming up to 65-70 ℃ and be incubated 25-35 minute then; Four, make the polypeptide protein emulsion: in above-mentioned polypeptide protein liquid, add beta-schardinger dextrin-and under 40-60 ℃, mixed 25-30 minute; Five, make mixed emulsion: mineral matter is made insatiable hunger Heshui solution together join in the polypeptide protein emulsion with whey powder and methionine then, under 40-60 ℃, mixed 15-25 minute then; Six, make concentrated emulsion: mixed emulsion 135-140 ℃ of following instantaneous sterilization, and then concentrate is formed the concentrated emulsion of solid content at 45-50%; Seven, water soluble vitamin is mixed with the aqueous solution that insatiable hunger closes and together join in the concentrated emulsion, puddles at normal temperatures then, homogeneous 15-25 minute with grease and fat soluble vitamin; Eight, the last spray-dried finished product of making.
2, the manufacture method of polypeptide calf meal according to claim 1 is characterized in that described water soluble vitamin is mixed by the raw material of following ratio of weight and number: VB1 is that 0.008-0.0085, VB2 are that 0.01-0.014, VB3 are that 0.016-0.018, VB11 are that 0.06-0.07, VB12 are 0.0001-0.0002 and aureomycin 0.5-1.
3, the manufacture method of polypeptide calf meal according to claim 1 is characterized in that described grease and fat soluble vitamin are mixed by the raw material of following ratio of weight and number: animal or plant grease 160-170, soybean lecithin 45-55, VA0.02-0.03, VD0.002-0.004, VE0.0001-0.0003 and antioxidant 0.1-0.5.
4, the manufacture method of polypeptide calf meal according to claim 3 is characterized in that described antioxidant is TBHQ.
5, the manufacture method of polypeptide calf meal according to claim 1 is characterized in that described mineral matter is mixed by the raw material of following ratio of weight and number: MgSO
4H
2O is 4-6, FeSO
4H
2O is 0.3-0.4, MnSO
4H
2O is 0.1-0.2, ZnSO
4H
2O is 0.12-0.13, CuSO
45H
2OO is that 0.04-0.05, KI are 0.0007-0.0009, CoCl
26H
2O is 0.0004-0.0006, Na2SeO
3For 0.0007-0.0008, KCl are 11-14, NaCO
3H
2O is 12-13, H
3PO
4Be 23-27, CaCl
2Be 25-35.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031325661A CN1493204A (en) | 2003-08-20 | 2003-08-20 | Manufacturing method of polypeptide calf milk powder substitute |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031325661A CN1493204A (en) | 2003-08-20 | 2003-08-20 | Manufacturing method of polypeptide calf milk powder substitute |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1493204A true CN1493204A (en) | 2004-05-05 |
Family
ID=34239867
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031325661A Pending CN1493204A (en) | 2003-08-20 | 2003-08-20 | Manufacturing method of polypeptide calf milk powder substitute |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692879B (en) * | 2009-10-23 | 2011-12-07 | 北京九州大地生物技术集团股份有限公司 | Antidysenteric early-weaning calf opening material |
| CN102488012A (en) * | 2011-12-14 | 2012-06-13 | 北京东方联鸣科技发展有限公司 | Formula milk powder for calves |
| CN102986877A (en) * | 2011-09-14 | 2013-03-27 | 光明乳业股份有限公司 | Production technology for formula milk powder |
| CN103283864A (en) * | 2013-06-17 | 2013-09-11 | 河北农业大学 | Dairy bull calf milk replacer for producing veal |
| CN103798390A (en) * | 2014-01-23 | 2014-05-21 | 甘南倍益特生物科技有限公司 | Yak cheese protein nutritional powder applicable to middle aged and elderly people and preparation method thereof |
| CN104489278A (en) * | 2015-01-05 | 2015-04-08 | 贵州大学 | Extremely-low milk protein milk replacer for calves under over-early weaning and preparation method of extremely-low milk protein milk replacer |
| CN105961844A (en) * | 2016-05-04 | 2016-09-28 | 沈喆如 | Feed with hydrolyzed protein and preparation method of feed |
| CN107897538A (en) * | 2017-12-08 | 2018-04-13 | 光明牧业有限公司 | A kind of complex intensifying calf meal and preparation method thereof |
-
2003
- 2003-08-20 CN CNA031325661A patent/CN1493204A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692879B (en) * | 2009-10-23 | 2011-12-07 | 北京九州大地生物技术集团股份有限公司 | Antidysenteric early-weaning calf opening material |
| CN102986877A (en) * | 2011-09-14 | 2013-03-27 | 光明乳业股份有限公司 | Production technology for formula milk powder |
| CN102986877B (en) * | 2011-09-14 | 2014-07-23 | 光明乳业股份有限公司 | Production technology for formula milk powder |
| CN102488012A (en) * | 2011-12-14 | 2012-06-13 | 北京东方联鸣科技发展有限公司 | Formula milk powder for calves |
| CN103283864A (en) * | 2013-06-17 | 2013-09-11 | 河北农业大学 | Dairy bull calf milk replacer for producing veal |
| CN103283864B (en) * | 2013-06-17 | 2015-01-28 | 河北农业大学 | Dairy bull calf milk replacer for producing veal |
| CN103798390A (en) * | 2014-01-23 | 2014-05-21 | 甘南倍益特生物科技有限公司 | Yak cheese protein nutritional powder applicable to middle aged and elderly people and preparation method thereof |
| CN103798390B (en) * | 2014-01-23 | 2015-10-28 | 甘南倍益特生物科技有限公司 | A kind of yak milk casein nutrient powder and preparation method thereof being applicable to the elderly |
| CN104489278A (en) * | 2015-01-05 | 2015-04-08 | 贵州大学 | Extremely-low milk protein milk replacer for calves under over-early weaning and preparation method of extremely-low milk protein milk replacer |
| CN105961844A (en) * | 2016-05-04 | 2016-09-28 | 沈喆如 | Feed with hydrolyzed protein and preparation method of feed |
| CN107897538A (en) * | 2017-12-08 | 2018-04-13 | 光明牧业有限公司 | A kind of complex intensifying calf meal and preparation method thereof |
| CN107897538B (en) * | 2017-12-08 | 2021-08-20 | 光明牧业有限公司 | Compound fortified calf milk replacer and preparation method thereof |
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