CN1489997A - Anticancer nutritive Chinese medicine - Google Patents
Anticancer nutritive Chinese medicine Download PDFInfo
- Publication number
- CN1489997A CN1489997A CNA021601046A CN02160104A CN1489997A CN 1489997 A CN1489997 A CN 1489997A CN A021601046 A CNA021601046 A CN A021601046A CN 02160104 A CN02160104 A CN 02160104A CN 1489997 A CN1489997 A CN 1489997A
- Authority
- CN
- China
- Prior art keywords
- lycopene
- alpha
- chinese medicine
- linolenic acid
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
A nutritive anticancer Chinese medicine is prepared from perilla seeds through extracting alpha-linolenic acid, purifying, and proportionally mixing it with lycopene. Its advantages are rich nutrients, high effect to suppress cancer, and no toxic by-effect.
Description
Technical field
The present invention relates to a kind of employing medicine, food dual-purpose Chinese crude drug Fructus Perillae is a raw material, the fatty acid alpha-linolenic acid is closed in the extraction insatiable hunger, through purifying after with purification after a kind of anticancer nutritional Chinese medicine of the compound composition of lycopene.
Background technology
According to domestic and international test, documents and materials and zoopery result, alpha-linolenic acid enters chain elongation in the human body, and enzymes metabolism is converted into EPA, DHA, and it decomposes fast, and the fat in the metabolism blood is regulated blood fat reducing, its 3 prostaglandin that is (TXA, PGI that derive
3) can blood vessel dilating, promote blood flow, suppress platelet aggregation, reach the effect of blood circulation promoting and blood stasis dispelling, mediation blood flow, hard masses softening and resolving.The active characteristics of alpha-linolenic acid, easily oxidation conversion is a linoleic acid, lycopene is very strong antioxidant, just can remedy the deficiency of alpha-linolenic acid.It is very capable that lycopene is removed peroxy radical, can improve people's autoimmune function, and it is a kind of carotenoid, has anti-cancer function.Domestic and international experiment, reported in literature: " lycopene obviously rising tune GJIC function, inhibition malignant cell is grown." about the anti-cancer mechanism of lycopene, Berrtram JS, et al.Carcinogenesis, 1991,12,2109～2114 grades are thought: " lycopene can promote to have and keeps the proteinic synthetic of the normal combination of intercellular substance.When cell generation canceration, intercellular substance is in conjunction with dying down.Because lycopene can strengthen the intercellular substance combination, so can effectively suppress canceration." Levin (1994) etc. discovers that " the cancerous cell increment that is caused by insulin-like growth factor (IGF) can be suppressed by lycopene." lycopene is to find just after the nineties that it has very high nutrition, has the various biological effect, it is nature antioxidation and the strongest material of defying age ability, Shi ﹠amp respectively; 3 times and 100 times of vitamin E of Szlig-carotin.
21st century is to return Piao to return very, payes attention to the exploitation of natural plant, the century of utilization.China's resources of medicinal plant is abundant, but fails effectively to protect and rational exploitation and utilization.Herbal plant resource also has and very most ofly to play the part of " colony role ", is also selling cheapness " medical material bavin standing grain ".The soup that the Chinese medicine compatibility is made, ball, powder considerable part, it is indeterminate to attack effective constituent, or without purification, does not have scientific quantitative and detection method and data, is difficult to enter the international market.
Summary of the invention
Purpose of the present invention is exactly natural resources Fructus Perillae and the Fructus Lycopersici esculenti that makes full use of China, adopts advanced extractive technique, extracts the effective ingredient lycopene and the alpha-linolenic acid that wherein contain, is prepared into the anticancer compound dietetic product.
The object of the present invention is achieved like this:
At first with the Fructus Perillae supercritical CO
2The extraction alpha-linolenic acid, extracting pressure is 30～40Mpa, and extraction temperature is 30～45 ℃, obtains unsaturated fatty acid, and wherein alpha-linolenic acid is 65% by unsaturated fatty acid relative percentage content.Again extract is put into the high low temperature experimental box of program control alternation, control temperature-30～-1 ℃, temperature-controlled precision ± 1 ℃, the temperature inside the box evenly ± 1 ℃, progressively heat up from-30 to-1 ℃, more than the maximum falling-rising temperature speed-30 ℃ 〉=3 ℃ separate the purification alpha-linolenic acids, obtain 65～85% alpha-linolenic acid;
With raw tomatoes material vacuum drying at room temperature, behind liquid nitrogen-196 ℃ freezing and pulverizing, carry out a vacuum sublimation again, carry out column chromatography for separation with the organic solvent eluting then, with the product that separation obtains, concentrating under reduced pressure or through supercritical CO
2Extraction, extracting pressure is 30～40Mpa, extraction temperature is 30～45 ℃, obtains the lycopene of 5～80% purity;
Raw material after two kinds of purifications is pressed lycopene and alpha-linolenic acid mass volume ratio: 1-110: 1-5; Unit: mg/ml.
Material purity: lycopene 5-80%, alpha-linolenic acid is 65-85% by unsaturated fatty acid relative percentage content, all can reach therapeutic effect to the weight various cancers.
We make anticancer nutritional Chinese medicine with alpha-linolenic acid and lycopene combination, to people's gastric cancer, intestinal cancer, breast carcinoma, the nude mice that the pernicious sarcoma of S180 etc. is done, (experimental result sees Table 1 to the mice animal experiment, 2,3,4) confirm really energy blood circulation promoting and blood stasis dispelling of this product, hypercoagulability in microcirculation improvement and the body, thereby promote the absorption of fibrous tissue, strengthen the intercellular substance combination, effectively suppress canceration, reach anticancer and the anticancer growth, we observe and take many batches of people's cancer tests of this product nude mice simultaneously, the feed normal type increases, refreshing, form is good, and this product is nutritious, and is nontoxic, side effect, can alleviate operation, chemotherapy, the toxic and side effects of radiotherapy and treating the poisonous disease with poisonous drugs anticarcinogen.
Table 1, the administration of this product oral are to the preliminary experiment therapeutic outcome (injection is waited to mend) of murine sarcoma S-180
| Group | Animal | Average weight | Average tumor is heavy *(g) ???(X±SD) | Tumour inhibiting rate (%) | ????P | ||
| Beginning | The end | Beginning | The end | ||||
| Distilled water 0.2ml/ only | ?10 | ?10 | ?18.8 | ?27.9 | ?2.25±0.49 | ?---- | -- |
| ?CTX30mg/kg | ?10 | ?10 | ?18.4 | ?23.1 | ?0.59±0.14 | ?73.8 | ＜0.001 |
| No. 0 0.1ml/10g | ?10 | ?10 | ?18.7 | ?27.2 | ?2.02±0.44 | ?10.2 | ＞0.05 |
| No. 1 0.2ml/10g | ?10 | ?10 | ?18.6 | ?28.1 | ?1.54±0.48 | ?31.6 | ＜0.01 |
| No. 1 0.1ml/10g | ?10 | ?10 | ?18.7 | ?27.7 | ?1.90±.053 | ?15.6 | ＞0.05 |
| No. 1 0.05ml/10g | ?10 | ?10 | ?19.2 | ?27.2 | ?1.76±0.46 | ?21.8 | ＞0.05 |
| ???? *X±SD???????????n＝10 | |||||||
Table 2, the administration of this product oral are to people's Gastric Cancer MGC-803 nude mice tumor experiment therapeutic outcome (injection is waited to mend)
| Group | Dosage and route of administration | Moving | Average weight | Average tumor is heavy *(g) | Tumour inhibiting rate (%) | ????P | ||
| Beginning | The end | Beginning | The end | |||||
| Distilled water | 0.2ml/ X28PO only | ?8 | ?8 | ?17.5 | ?23.0 | ?1.55±0.48 | ?---- | ---- |
| No. 1 (H) | 0.2ml/10gX28PO | ?8 | ?7 | ?17.9 | ?23.9 | ?0.94±0.52 | ?39.4 | ＜0.05 |
| No. 1 (M) | 0.1ml/10gX28PO | ?8 | ?8 | ?17.8 | ?24.4 | ?1.05±0.24 | ?32.3 | ＞0.05 |
| No. 1 (L) | 0.05ml/10gX28PO | ?8 | ?8 | ?17.2 | ?23.1 | ?1.30±0.77 | ?16.1 | ＜0.05 |
| ???? *X±SD?????????n＝8 | ||||||||
Table 3, the administration of this product oral are to experimental therapy result's (injection is waited to mend) of people's intestinal cancer nude mice HCT-8
| Group | Dosage and route of administration | Number of animals | Average weight | Average tumor is heavy *(g) ???(X±SD) | Tumour inhibiting rate (%) | ???P | ||
| Beginning | The end | Beginning | The end | |||||
| Distilled water | 0.2ml/ X20PO only | ?8 | ?8 | ?20.4 | ?22.4 | ?3.81±0.38 | ?---- | ---- |
| ?5-FU | 20mg/kgX10ip | ?8 | ?8 | ?20.6 | ?20.9 | ?1.69±0.61 | ?55.6 | ＜0.001 |
| No. 1 (H) | 0.2ml/10gX20PO | ?8 | ?8 | ?20.8 | ?23.1 | ?2.30±0.42 | ?39.6 | ＜0.001 |
| No. 1 (M) | 0.1ml/10gX20PO | ?8 | ?8 | ?20.4 | ?20.9 | ?2.79±0.51 | ?26.8 | ＜0.001 |
| No. 1 (L) | 0.05ml/10gX20PO | ?8 | ?8 | ?20.7 | ?21.1 | ?3.11±1.03 | ?18.4 | ＞0.05 |
| ???? *X±SD??????????n＝8 | ||||||||
Table 4, the administration of this product oral are to human breast carcinoma MGE-7 nude mice oncotherapy experimental result (injection is waited to mend)
| Group | Dosage and route of administration | Animal | On average | Average tumor is heavy *(g) ????(X±SD) | Tumour inhibiting rate (%) | ???P | ||
| Beginning | The end | Beginning | The end | |||||
| Distilled water | 0.2ml/ X39PO only | ?7 | ?7 | ?18. | 23.1 | ?1.21±0.16 | ?---- | ---- |
| No. 1 (H) | 0.2ml/10gX39PO | ?7 | ?7 | ?18. | 25.0 | ?0.69±0.11 | ?43.0 | ＜0.05 |
| No. 1 (M) | 0.1ml/10gX39PO | ?7 | ?7 | ?18. | 25.1 | ?0.84±0.15 | ?30.6 | ＞0.05 |
| No. 1 (L) | 0.05ml/10gX39PO | ?7 | ?7 | ?18. | 23.8 | ?0.67±0.11 | ?44.6 | ＜0.05 |
| ?????? *X±SD????????n＝7 | ||||||||
This product oral and drug administration by injection all have therapeutical effect to tumors such as human prostata cancer, oral cancer, cancer of pancreas, pulmonary carcinoma.
The specific embodiment
Embodiment 1
Get 1 kilogram of raw tomatoes material, the room temperature vacuum drying, reuse liquid nitrogen-196 ℃ freezing and pulverizing carries out a vacuum sublimation again, is dried to powder, obtain dry powder 100-200 gram, get 10 grams and add in the silicagel columns, carry out column chromatography for separation, the product concentrating under reduced pressure that separation is obtained with the ether eluting, obtain 400 milligrams of lycopene raw materials, purity is 20%.With 13 kilograms Fructus Perillae, through critical CO
2Extraction, extracting pressure is 30Mpa, and extraction temperature is 35 ℃, obtains 5.2 kilograms of insatiable hungers and closes fatty acid, utilizes the high low temperature experimental box of program control alternation again, and the control temperature progressively heats up by-30 ℃ to-1 ℃ purification alpha-linolenic acids, and obtaining purity is 70% alpha-linolenic acid.Get 50 milligrams of the lycopenes of preparation, 5 milliliters of alpha-linolenic acids are used ultrasonic mixing, make compound spice of the present invention, after the sealing, use for making required dosage form.
Embodiment 2
Get 20 kilograms raw tomatoes material, the room temperature vacuum drying, reuse liquid nitrogen-196 ℃ freezing and pulverizing, carry out a vacuum sublimation again, be dried to powder, obtain 2000-4000 gram dry powder, get 100 gram adding silicagel columns and carry out column chromatography for separation with the acetone eluting, with the product concentrating under reduced pressure that separation obtains, obtain 1.5 gram lycopene raw materials, purity is 60%.With 30 kilograms Fructus Perillae, through critical CO
2Extraction, extracting pressure 40Mpa, 40 ℃ of extraction temperature obtain 12 kilograms of insatiable hungers and close fatty acid, utilize the high low temperature experimental box of program control alternation again, and the control temperature progressively heats up by-30--1 ℃, and the purification alpha-linolenic acid obtains purity and is 73% alpha-linolenic acid.Get 60 milligrams of the lycopenes of preparation, 1 milliliter of alpha-linolenic acid is used ultrasonic mixing, makes compound medicine raw material of the present invention, after the sealing, uses for making required dosage form.
Embodiment 3
Get 100 kilograms raw tomatoes material, the room temperature vacuum drying, reuse liquid nitrogen-196 ℃ freezing and pulverizing carries out a vacuum sublimation again, is dried to powder, obtains 10-20 kilogram dry powder, through supercritical CO
2Extraction, extracting pressure is 40Mpa, and extraction temperature is 45 ℃, obtains 5.5 gram lycopene raw materials, and purity is 15%.With 13000 kilograms Fructus Perillae, in batches through supercritical CO
2Extraction, extracting pressure 40Mpa, 45 ℃ of extraction temperature obtain 5000 kilograms of insatiable hungers and close fatty acid, utilize the high low temperature experimental box of program control alternation again, and the control temperature progressively heats up by-30 to-1 ℃ of purification alpha-linolenic acids, obtains purity and be 75% alpha-linolenic acid.Get lycopene 11 grams of preparation, 100 milliliters of alpha-linolenic acids with they mixings, make the compound spice of the present invention with ultrasonic, seal stand-by.
Embodiment 4
Get 100 kilograms raw tomatoes material, the room temperature vacuum drying, reuse liquid nitrogen-196 ℃ freezing and pulverizing carries out a vacuum sublimation again, is dried to powder, obtains 10-20 kilogram dry powder, gets 10 kilograms, uses supercritical extraction CO
2, extracting pressure is 40Mpa, and 45 ℃ of extraction temperature obtain 700 gram lycopene raw materials, and purity is 13%.Get lycopene 100 grams of preparation,, with they mixings, make the compound spice of the present invention, seal stand-by with ultrasonic with 1000 milliliters of the alpha-linolenic acids that makes with embodiment 1.
Embodiment 5-9
Prepare lycopene and alpha-linolenic acid raw material with the method identical, it pressed following amount mixing with embodiment 1-4,
Lycopene/alpha-linolenic acid: 2kg/100L
Lycopene/alpha-linolenic acid: 6kg/100L
Lycopene/alpha-linolenic acid: 6kg/500L
Lycopene/alpha-linolenic acid: 8kg/100L
Lycopene/alpha-linolenic acid: 10kg/100L
Lycopene purity 5-80%
Alpha-linolenic acid purity 65-85%
All can reach cancer is had therapeutic effect.
Claims (6)
1, a kind of anticancer nutritional Chinese medicine is characterized in that: it is to be raw material by the Chinese crude drug Fructus Perillae, and the fatty acid alpha-linolenic acid is closed in the extraction insatiable hunger, through purifying after with purification after lycopene combine.
2, a kind of anticancer nutritional Chinese medicine according to claim 1 is characterized in that: lycopene, alpha-linolenic acid is 1-110: 1-5 by mass volume ratio, unit: mg/ml.
3, a kind of anticancer nutritional Chinese medicine according to claim 1 is characterized in that: the lycopene purity that participates in forming is 5-80%.
4, a kind of anticancer nutritional Chinese medicine according to claim 1 is characterized in that: the purity that participates in the alpha-linolenic acid of composition is 65-85% by unsaturated fatty acid relative percentage content.
5, a kind of anticancer nutritional Chinese medicine according to claim 1, it is characterized in that: alpha-linolenic acid is with the Fructus Perillae supercritical CO
2Extraction, extracting pressure is 30-40Mpa, and extraction temperature is 30-45 ℃, and heating up gradually to purify to-1 ℃ in temperature-30 then obtains.
6, a kind of anticancer nutritional Chinese medicine according to claim 1, it is characterized in that: lycopene is with raw tomatoes material vacuum drying at room temperature, through liquid nitrogen-196 ℃ lyophilization Cheng Fenhou, carry out a vacuum sublimation again, use the organic solvent eluting then, carry out column chromatography for separation, the product concentrating under reduced pressure that separation is obtained, or with raw tomatoes material through supercritical CO
2Extraction, extracting pressure is 30-40Mpa, extraction temperature is 30-45 ℃ of acquisition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021601046A CN1194680C (en) | 2002-10-15 | 2002-12-31 | Anticancer nutritive Chinese medicine |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02130896.9 | 2002-10-15 | ||
| CN02130896 | 2002-10-15 | ||
| CNB021601046A CN1194680C (en) | 2002-10-15 | 2002-12-31 | Anticancer nutritive Chinese medicine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1489997A true CN1489997A (en) | 2004-04-21 |
| CN1194680C CN1194680C (en) | 2005-03-30 |
Family
ID=34195415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021601046A Expired - Fee Related CN1194680C (en) | 2002-10-15 | 2002-12-31 | Anticancer nutritive Chinese medicine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1194680C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1319534C (en) * | 2004-10-28 | 2007-06-06 | 昆明翔昊科技有限公司 | Lycopene and coenzyme Q#-[10] containing formulation and its manufacturing method |
| CN100348209C (en) * | 2004-07-08 | 2007-11-14 | 乌鲁木齐优康来科技开发有限公司 | Natural lycopene compound oil preparation and capsule and its preparing method and use |
| CN100350924C (en) * | 2005-12-29 | 2007-11-28 | 颜怀伟 | Product for improving resistance and preventing senile and its manufacturing method |
| CN100353956C (en) * | 2005-12-29 | 2007-12-12 | 颜怀伟 | Nutritire product for preventing and treating cardiac and cerebral vascular system disease |
| CN100353955C (en) * | 2005-12-29 | 2007-12-12 | 颜怀伟 | Nutritire food for antisenile and anticancer and its manufacturing method |
| CN100435812C (en) * | 2005-12-29 | 2008-11-26 | 颜怀伟 | Compound antioxidation nutritive food and its manufacturing method |
| CN100544711C (en) * | 2004-04-23 | 2009-09-30 | 昆明翔昊科技有限公司 | A kind of formulation product of prevention of prostatic hyperplasia |
| CN102389111A (en) * | 2011-10-29 | 2012-03-28 | 李南 | Compound edible powder with enhanced effect of preventing and inhibiting cancer |
-
2002
- 2002-12-31 CN CNB021601046A patent/CN1194680C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100544711C (en) * | 2004-04-23 | 2009-09-30 | 昆明翔昊科技有限公司 | A kind of formulation product of prevention of prostatic hyperplasia |
| CN100348209C (en) * | 2004-07-08 | 2007-11-14 | 乌鲁木齐优康来科技开发有限公司 | Natural lycopene compound oil preparation and capsule and its preparing method and use |
| CN1319534C (en) * | 2004-10-28 | 2007-06-06 | 昆明翔昊科技有限公司 | Lycopene and coenzyme Q#-[10] containing formulation and its manufacturing method |
| CN100350924C (en) * | 2005-12-29 | 2007-11-28 | 颜怀伟 | Product for improving resistance and preventing senile and its manufacturing method |
| CN100353956C (en) * | 2005-12-29 | 2007-12-12 | 颜怀伟 | Nutritire product for preventing and treating cardiac and cerebral vascular system disease |
| CN100353955C (en) * | 2005-12-29 | 2007-12-12 | 颜怀伟 | Nutritire food for antisenile and anticancer and its manufacturing method |
| CN100435812C (en) * | 2005-12-29 | 2008-11-26 | 颜怀伟 | Compound antioxidation nutritive food and its manufacturing method |
| CN102389111A (en) * | 2011-10-29 | 2012-03-28 | 李南 | Compound edible powder with enhanced effect of preventing and inhibiting cancer |
| CN102389111B (en) * | 2011-10-29 | 2014-02-12 | 李南 | Compound edible powder with enhanced effect of preventing and inhibiting cancer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1194680C (en) | 2005-03-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107114783B (en) | High-selenium nutritional supplement for enhancing immunity and assisting tumor recovery | |
| CN112089051A (en) | Nutritional composition for improving body inflammatory signs of tumor patients and preparation method thereof | |
| CN1273839A (en) | Transfer and modification of intestinalprobiotics fermentation culture medium to natural medicine | |
| CN1194680C (en) | Anticancer nutritive Chinese medicine | |
| CN101724667A (en) | Technology for extracting dendrobium polysaccharide by applying biology engineering enzyme technology and product thereof and application | |
| CN103141702A (en) | Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea | |
| CN113061195A (en) | Compound polysaccharide with blood fat reducing effect and preparation method and application thereof | |
| CN103877122A (en) | Production method and donkey-hide gelatin of Chinese herbal donkey-hide gelatin material | |
| CN104940549B (en) | A kind of Chinese herbal medicine formula for large-scale meat duck lipid-loweringing | |
| CN103719855B (en) | Nutritious food and production method thereof | |
| CN104872646A (en) | Non-full-nutrition formula food for patients with colorectal cancers | |
| CN115844937B (en) | Preparation method and application of herba Sonchi Oleracei extract with anti-hangover and brain protecting effects | |
| CN100574780C (en) | The compositions of anti-inflammatory process | |
| CN107373643A (en) | A kind of ferment cream and preparation method thereof | |
| CN103734767A (en) | Health-care food | |
| CN1840011A (en) | Chinese medicine for treating prostate disease | |
| CN102228181B (en) | Composition eaten by cancer patients and containing oligopeptide and chitosan oligosaccharide | |
| CN101797049B (en) | Nutrient eight-treasure soup formula and preparation process thereof | |
| CN104906140A (en) | Donkey-hide gelatin, a preparation method and associated equipment | |
| CN104825817B (en) | A kind of Chinese medicine preparation with antitumor action | |
| CN108014301A (en) | A kind of Chinese medicine ferment for treating leukaemia and preparation method thereof | |
| CN111557407A (en) | Health food capable of reducing blood fat | |
| CN1146915A (en) | Compound anticancer, its preparing process and use | |
| CN114424825B (en) | Quintuple fat-reducing composition and preparation method and application thereof | |
| CN114507297A (en) | Preparation and application of inonotus obliquus polysaccharide extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050330 Termination date: 20161231 |