CN1146915A - Compound anticancer, its preparing process and use - Google Patents
Compound anticancer, its preparing process and use Download PDFInfo
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- CN1146915A CN1146915A CN 94112264 CN94112264A CN1146915A CN 1146915 A CN1146915 A CN 1146915A CN 94112264 CN94112264 CN 94112264 CN 94112264 A CN94112264 A CN 94112264A CN 1146915 A CN1146915 A CN 1146915A
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- semen coicis
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Abstract
An anticancer composition contains coix seed polyose (0.5-4.7 mg/ml), yam polyose (0.9-7 mg/ml), pachyman (0.7-2 mg/ml), coixenolide (0.05-0.9 mg/ml), pachymic acid (0.01-0.08 mg/ml) and polystictin (0.3-10 mg/ml), and can serve to improve immunity and increase leucocytes for patient after chemicotherapy.
Description
The present invention relates to a kind of novel pharmaceutical combination thing-compound anticancer that can improve body immunity rapidly and increase chemotherapy patients's leukocyte level, the present invention also relates to the preparation method and its usage of said composition.
Cancer is at present to one of disease of human life and health threat maximum, people are striving to find various methodologies always and are effecting a radical cure this disease, but the frequent at present method of using, as chemotherapy, radiotherapy, operation thing remove, photochemotherapy etc. all has the immunity degradation of making us body, and the big shortcoming of toxic and side effects.
Scholars think that cancerous cell is to come from normal cell to be subjected to the result that certain triggering factor stimulates and generation makes a variation; Many data reports are also arranged, cancer mainly is because the sudden change of human body cell producer, cause cell to divide fast, eventually to canceration, autoimmunity descends simultaneously, can not in time remove cancerous tumor cell or the like, thereby improves the human body immunity, the vigor of enhance immunity cell, it is vital keeping organic metabolic balance.
Effective ingredient in some traditional Chinese herbal medicine of China can be given full play to human immune system's mechanism to a great extent, improves the immunity of human body, thereby can slow down further developing and worsening of cancer to greatest extent, and the effect that takes stopgap measures and effect a permanent cure is arranged.
According to record, Semen Coicis is analgesic except having, the pharmacological action of analgesic, cancerous cell is had certain inhibitory action; Rhizoma Dioscoreae has pharmacological actions such as the immunologic function of adjusting, dirty tumor, blood pressure lowering; Poria and prepared from coriolus versicolor mycelium also have certain anti-tumor effect and can improve immune function of human body.
Though the pharmacological action of these Chinese medicines is known, because the composition complexity of Chinese medicine all has certain difficulty to obtaining through refining of active ingredient, so the goods of the anti-curing cancers made from Chinese medicine that goes on the market at present are less.Up to now, existing at home and abroad wait with polysaccharide-peptide preparation and Germinatus Phragmitis goods treat, prophylaxis of cancer but this based article draw materials single, the effectiveness volume is narrow, effect is limited, so be restricted in application.Therefore, the utmost point compositions that need develop a kind of novelty improves body immunity and anti-cancer ability thereof; Simultaneously, can reduce patient again to a great extent, promote the raising of patient's leukocyte level, thereby make this persistent ailment of cancer obtain effectively preventing because of a series of side effect or the complication of performing the operation and chemotherapy causes.
An object of the present invention is to provide and a kind ofly can improve immunity, anti-cancer ability especially, and can increase the compositions-compound cancer gram of the novelty of chemotherapy patients's leukocyte level.
Another object of the present invention provides a kind of method for preparing compound anticancer.
A further object of the present invention provides the purposes of compound anticancer on Pharmaceutical and grocery trade.
The objective of the invention is to realize: a kind of compositions by following design, comprise 0.5-4.7mg/ml Semen Coicis polysaccharide, 0.9-7mg/ml Chinese yam polysaccharide, 0.1-2mg/ml pachyman, 0.05-0.9mg/ml Semen Coicis fat, 0.01-0.08mg/ml pachymic acid and 0.3-10mg/ml polystictin.
Compound anticancer can also contain further that lecithin, several amino acids, vitamin and other strengthening the spleen and stomach, lung benefiting kidney, antiinflammatory are quenched the thirst, the medicinal herb components of timid cold, the blood fat reducing of dampness removing.
Compound anticancer of the present invention makes by following steps:
(A) fermentation engineering: make the bacterial strain of Coriolous Dersicolor (Fr.) Quel B-12 and anticancer epiphyte C-18 in natural liquid culture medium, make shaken cultivation, to obtain the mycelium materials A;
(B) extracting effective ingredient: make the mycelium materials A centrifugal, separate obtaining precipitation and supernatant (B);
(C) content with each active princlple in the compositions is the proportioning that Rhizoma Dioscoreae, Semen Coicis and Poria powder are calculated in the basis, mixing the back mixes with per kilogram Chinese medicine adding 10 ratios of going up clear liquid C, boil extracting, the cooling back is centrifugal, concentrated supernatant, make the compound anticancer mixed solution, comprising 0.5-4.7mg Semen Coicis polysaccharide, 0.9-7mg/ml Chinese yam polysaccharide, 0.1-2mg/ml pachyman, 0.05-0.9mg/ml Semen Coicis fat, 0.01-0.08mgml pachymic acid and 0.3-10mg/ml polystictin.
The precipitation that obtains in step (B) can be again through freezing, and drying is pulverized, and uses the distilled water extracting, is preferably extracting three times, and the supernatant of gained is incorporated in the supernatant (B) again.
In step (C), the mixture of described Chinese herbal medicine and mycelium supernatant can be repeated extracting three times, merges supernatant and promptly obtains the compound anticancer mixed solution.
Described natural liquid culture medium is the known natural mediumes that do not contain any additive and inorganic compound of this technical field personnel, as agar Nutrient medium etc.
The application of described compound anticancer mixture solution, comprising bottles makes medical health protection product and/or food additive.
Through precipitate with ethanol, cold putting spent the night with the compound anticancer mixture solution, centrifugal collecting precipitation, and precipitation is dissolved with suitable quantity of water, and repurity concentrates, and obtains powder G after the lyophilization.
Described purification process comprises the known technology of this technical field, as dialysis, gel filtration and ion-exchange chromatography etc.
Described precipitate with ethanol purification process can repeat 2-4 time.
Technology with the pharmaceuticals industry routine can be made powder G tablet, capsule or injection.
More particularly, compound anticancer compositions of the present invention comprises 0.5-4.7mg/ml Semen Coicis polysaccharide, 0.9-7mg/ml Chinese yam polysaccharide, 0.1-2mg/ml pachyman, 0.05-0.9mg/ml Semen Coicis fat, 0.01-0.08mg/ml pachymic acid and 0.3-10mg/ml polystictin.
Said composition preferably includes 2mg/ml Semen Coicis polysaccharide, 3mg/ml Chinese yam polysaccharide, 0.8mg/ml polystictin, the high sugar of 0.06mg/ml Poria, 0.4mg/ml Semen Coicis fat and pachymic acid 0.03mg/ml.
Wherein said polysaccharide and polystictin contain polysaccharide and glycopeptide material such as β (1-3), β (1-4) and β (1-6) glucosan.
Described compound anticancer makes by following steps: (A) be seeded to mycelium inclined-plane solid dark culturing under the bacterial strain aseptic condition with Coriolous Dersicolor (Fr.) Quel B-12 and anticancer epiphyte C-18, aseptic inoculation is to the fermentation tank that contains natural liquid culture medium again, under 23-29 ℃, carried out shaken cultivation 6-14 days with 300 rev/mins, obtain the mycelium materials A: (B) shown in flow process I, make the mycelium materials A through centrifugal supernatant (B) and the precipitate of obtaining of 4000rpm, comparatively preferably precipitate repeats freezing, dry, pulverize, the step of decocting in water, merging obtains supernatant (B); (C) go out the optimization proportioning of Rhizoma Dioscoreae, Semen Coicis, Poria by the cubage of each composition in the compositions, pulverize separately, cross 80 mesh sieves, mix then, go up the ratio of clear liquid (B) with per kilogram Chinese medicine adding 16, the mixing of feeding in raw material, boil extracting, cool off centrifugal (4000rpm), triplicate, merge supernatant, obtain mixed solution (F) after concentrating.
Prepared compound anticancer of the present invention can be used for Pharmaceutical and grocery trade.Specifically, according to flow process II, mixed solution (F) can directly be produced Medicines and Health Product aborning, or directly adds as food additive when making food;
Perhaps mixed solution (F) adds ethanol, refrigerator overnight, centrifugal collecting precipitation, behind water dissolution, dialyse, through gel filtration, separate, purification with ion-exchange chromatography, again through concentrating, add ethanol, refrigerator overnight, centrifugal collecting precipitation, it is inferior to give a baby a bath on the third day after its birth with dehydrated alcohol, after ether is washed once, precipitation is carried out lyophilization, obtain powdered complex (G).
Described powder G granulates with known technology on the pharmacy industry, makes tablet and/or capsule then.
Described powder G also can make injection through sterilization with after removing thermal source by the routine techniques of system injection.
Below in conjunction with specific embodiment the present invention is done into detailed elaboration down:
Embodiment 1
The mixed liquor adding of 200ml fungus B-12 and C-18 is contained in the fermentation tank of the natural culture fluid of 2000ml (fermentation liquid), velocity fluctuation with 300 rev/mins under the dark condition was cultivated 8 days, make fermentation liquid, centrifugal with 4000rpm, collect supernatant, precipitation is boiled extracting three times through smashing the adding deionized water to pieces, merge supernatant, obtain 2600ml.With the 260g Semen Coicis, the 100g Rhizoma Dioscoreae, 50g Poria pulverize separately is crossed 80 mesh sieves, pours into then in the supernatant, mixes back steaming and decocting 8-10 hour.Centrifugal, precipitate with deionized water extracting three times repeatedly, merge supernatant after, the 5000ml semifinished product.
Embodiment 2
Except 200ml fungus mixed liquor is added in the fermentation tank that contains the 12000ml fermentation liquid, and the input amount of Chinese herbal medicine is 150 gram Semen Coiciss, 50 Keshan medicines, beyond the 20 gram Poria, the step that other repeats embodiment 1 obtains obtaining highly finished product through column chromatogram chromatography again under the 3800ml supernatant.
Embodiment 3
Method according to embodiment 1 makes the 10000ml mycelium fermentation broth.Speed with 4000rpm is centrifugal, obtains the 9800ml supernatant, inwardly adds 900g Semen Coicis, 600g Rhizoma Dioscoreae, 300g Poria through pulverizing, boils 8-10 hour, and is centrifugal, adds clear water again with medicinal residues extracting secondary, merges supernatant, gets mixed solution F12100ml.
Embodiment 4-6
According to following inventory, prepare cancer gram solution of the present invention by the step of embodiment 3.
Embodiment sequence number Semen Coicis Rhizoma Dioscoreae Poria fermentation liquid mixed solution core
(gram) (gram) (supernatant) be (ml) (ml)
4???????????450??????300?????100????????????9700????10900
5???????????275??????150?????50?????????????9880????10280
6 1,500 1,000 500 9,820 13700 cell in vitro are learned horizontal culture experiment:
From suffering from gonadoma disease (A
549), in the cancer human body of gastric cancer (SGC) and cervical cancer (Hela), take out A
549, SGC and Hela cell, respectively prepared from coriolus versicolor mycelium sporophore, shiitake mushroom hypha, anti-C-18 and cancer of the present invention gram are applied on the described cancerous cell, spend 72 hours after, observe the growth inhibition ratio of cancerous cell.
Pharmacological testing in the body:
| Polysaccharide origin | Growth inhibited % | ||
| ????A 549 | ????SGC | ????Hela | |
| The anti-C-18 of the anti-C-18 of prepared from coriolus versicolor mycelium fructification shiitake mushroom hypha (in the born of the same parents) shiitake mushroom hypha (born of the same parents are outer) (in the born of the same parents) (born of the same parents are outer) cancer gram | ????65.9 ????42.3 ????42.0 ????60.8 ????61.0 ????88.0 ????89.0 | ????32.0 ????45.1 ?????- ????48.0 ?????- ????53.0 ????79.0 | ????71.7 ????31.5 ?????- ?????- ?????- ?????- ????84.0 |
Randomly draw 6 cancer patients, wherein 2 examples have been done the lobectomy of lungs operation because of suffering from pulmonary carcinoma, have made chemotherapy after the operation; One example is a gastric cancer; One routine rhinocarcinoma; 2 routine colon cancer.After all these patient's underwent operative or the chemotherapy, body constitution is good for a long time, and the food of receiving is poor, and leukocyte obviously reduces, and takes compound anticancer after 2 months, the diet increase, and particularly the horizontal rebound significantly of patient's leukocyte can reach about 4000-5000 basically.
Compound anticancer of the present invention is that strain excellent is fermented through Fermentation Engineering, and the recycling biochemical technology carries out separation and purification, and a kind of compound substance that is composited through biochemical means with some composition of Chinese herbal medicine. In fungi culture medium, do not add the materials such as any inorganic compound ion, be conducive to keeping of human homergy's balance. The inside and outside test of body shows that cancer gram composition of the present invention has following advantages and purposes.
1. compound anticancer of the present invention has significant anticancer effect, can be used as the paramedicines of other treatment cancer; Also can use separately, long-term taking has better effect.
2. this product is through chemotherapy patients's long-term taking, and discovery can increase white blood cell count, keeps its horizontal stable, reduces the various side reactions that chemotherapy causes, improve immunocompetence, thereby this product is the desirable Medicines and Health Products of various chemotherapy patients.
3. it is antibiotic that this product has quench, improves human immunologic function's effect comprehensively, and being conducive to increases the resistance of multiple germ, reduces pathogenic toxin to the harm of human body, and the effect of play diseases prevention, curing the disease is senior health and fitness's maintenance god.
4. this product contains reducing blood lipid, and the active ingredients such as the invigorating the spleen of quenching one's thirst, beneficial lung can be improved illness greatly to relevant patient's drinking of said product.
Claims (9)
1. compound anticancer compositions, it comprises 0.5-4.7mg/ml Semen Coicis polysaccharide, 0.9-7mg/ml Chinese yam polysaccharide, 0.1-2mg/ml pachyman, 0.05-0.9mg/ml Semen Coicis fat, 0.01-0.08mg/ml pachymic acid and 0.3-10mg/ml polystictin.
2. compound anticancer compositions according to claim 1, it comprises 2mg Semen Coicis polysaccharide, 3mg/ml Chinese yam polysaccharide, 0.8mg/ml polystictin, 0.06mg/ml pachyman, 0.4mg/ml Semen Coicis fat and pachymic acid 0.03mg/ml.
3. compound anticancer compositions according to claim 1 and 2, it further contains, and lecithin, several amino acids, vitamin and other strengthening the spleen and stomach, lung benefiting kidney, antiinflammatory are quenched the thirst, the Chinese herbal medicine composition of timid cold, the blood fat reducing of dampness removing.
4. compound anticancer preparation of compositions method, it may further comprise the steps:
(A) fermentation engineering: make the bacterial strain of Coriolous Dersicolor (Fr.) Quel B-12 and anticancer epiphyte C-18 in natural liquid culture medium, make shaken cultivation, to obtain the mycelium materials A;
(B) extracting effective ingredient: make the mycelium materials A centrifugal, separate obtain precipitation and with clear liquid (B);
(C) content with each active princlple in the compositions is the proportioning that Rhizoma Dioscoreae, Semen Coicis and Poria powder are calculated in the basis, mixing the back mixes with per kilogram Chinese medicine adding 10 ratios of going up clear liquid C, boil extracting, the cooling back is centrifugal, concentrated supernatant, make the compound anticancer mixed solution, comprising 0.5-4.7mg Semen Coicis polysaccharide, 0.9-7mg/ml Chinese yam polysaccharide, 0.1-2mg/ml pachyman, 0.05-0.9mg/ml Semen Coicis fat, 0.01-0.08mgml pachymic acid and 0.3-10mg/ml polystictin.
5. method according to claim 4, wherein
The precipitation that obtains in step (B) can be again through freezing, and drying is pulverized, and with distilled water extracting three times, the supernatant of gained is incorporated in the supernatant (B) again.
6. method according to claim 4, wherein
In step (C), the mixture of described Chinese herbal medicine and mycelium supernatant can be repeated extracting three times, merges supernatant and promptly obtains the compound anticancer mixed solution.
7. method according to claim 4, natural liquid culture medium wherein is a natural plants composite powder 1.5%.Sucrose 2%.Animal proteinum extract 0.05% (V/V).
8. the application of a compound anticancer compositions, it comprises directly makes Medicines and Health Product and/or food additive with the described product of claim 1.
9. the application of a compound anticancer compositions comprises the further precipitate with ethanol of the described product of claim 1, lyophilization, and the powder of gained is made into tablet, capsule and/or injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94112264 CN1146915A (en) | 1994-08-17 | 1994-08-17 | Compound anticancer, its preparing process and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94112264 CN1146915A (en) | 1994-08-17 | 1994-08-17 | Compound anticancer, its preparing process and use |
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| Publication Number | Publication Date |
|---|---|
| CN1146915A true CN1146915A (en) | 1997-04-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94112264 Pending CN1146915A (en) | 1994-08-17 | 1994-08-17 | Compound anticancer, its preparing process and use |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1535619A1 (en) * | 2003-11-25 | 2005-06-01 | Sinphar Pharmaceutical Co., Ltd. | Poria extract devoid of secolanostane derivatives comprising lanostane derivatives for immunity enhancement |
| CN102988828A (en) * | 2012-11-21 | 2013-03-27 | 光明食品集团云南石斛生物科技开发有限公司 | Preparation method of dendrobium officinale granula |
| CN104509765A (en) * | 2014-12-04 | 2015-04-15 | 黄亚辉 | Immunity improving drug and preparation process thereof |
| CN112425779A (en) * | 2020-11-02 | 2021-03-02 | 安徽金源药业有限公司 | Poria cocos and Chinese yam polysaccharide particles and application thereof |
| CN117137137A (en) * | 2023-10-11 | 2023-12-01 | 浙江省中医院、浙江中医药大学附属第一医院（浙江省东方医院） | Dietary formula for hematopoietic stem cell transplantation patient to eat, preparation method and application thereof |
-
1994
- 1994-08-17 CN CN 94112264 patent/CN1146915A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1535619A1 (en) * | 2003-11-25 | 2005-06-01 | Sinphar Pharmaceutical Co., Ltd. | Poria extract devoid of secolanostane derivatives comprising lanostane derivatives for immunity enhancement |
| CN102988828A (en) * | 2012-11-21 | 2013-03-27 | 光明食品集团云南石斛生物科技开发有限公司 | Preparation method of dendrobium officinale granula |
| CN104509765A (en) * | 2014-12-04 | 2015-04-15 | 黄亚辉 | Immunity improving drug and preparation process thereof |
| CN112425779A (en) * | 2020-11-02 | 2021-03-02 | 安徽金源药业有限公司 | Poria cocos and Chinese yam polysaccharide particles and application thereof |
| CN117137137A (en) * | 2023-10-11 | 2023-12-01 | 浙江省中医院、浙江中医药大学附属第一医院（浙江省东方医院） | Dietary formula for hematopoietic stem cell transplantation patient to eat, preparation method and application thereof |
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