CN1482236A - Chrome-enriched yeast production method from edible yeast - Google Patents
Chrome-enriched yeast production method from edible yeast Download PDFInfo
- Publication number
- CN1482236A CN1482236A CNA031354912A CN03135491A CN1482236A CN 1482236 A CN1482236 A CN 1482236A CN A031354912 A CNA031354912 A CN A031354912A CN 03135491 A CN03135491 A CN 03135491A CN 1482236 A CN1482236 A CN 1482236A
- Authority
- CN
- China
- Prior art keywords
- chromium
- yeast
- organic
- rich
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 title claims abstract description 75
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 48
- 238000004519 manufacturing process Methods 0.000 title claims description 8
- 229910052804 chromium Inorganic materials 0.000 claims abstract description 73
- 239000011651 chromium Substances 0.000 claims abstract description 73
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000002360 preparation method Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000004471 Glycine Substances 0.000 claims abstract description 8
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims abstract description 8
- WYYQVWLEPYFFLP-UHFFFAOYSA-K chromium(3+);triacetate Chemical compound [Cr+3].CC([O-])=O.CC([O-])=O.CC([O-])=O WYYQVWLEPYFFLP-UHFFFAOYSA-K 0.000 claims abstract description 5
- 238000006243 chemical reaction Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 229960003512 nicotinic acid Drugs 0.000 claims abstract description 4
- 235000001968 nicotinic acid Nutrition 0.000 claims abstract description 4
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 4
- 230000008569 process Effects 0.000 claims abstract description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 38
- 239000012452 mother liquor Substances 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 150000001875 compounds Chemical class 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 5
- HPCCGRCEBFBZQP-UHFFFAOYSA-N chromium;pyridine-3-carboxylic acid Chemical compound [Cr].OC(=O)C1=CC=CN=C1 HPCCGRCEBFBZQP-UHFFFAOYSA-N 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000007791 liquid phase Substances 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 3
- 239000007790 solid phase Substances 0.000 claims description 3
- 230000007704 transition Effects 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 150000001845 chromium compounds Chemical class 0.000 abstract 1
- 230000001276 controlling effect Effects 0.000 abstract 1
- 235000013402 health food Nutrition 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000007689 inspection Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 238000011514 vinification Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 239000002775 capsule Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 210000005253 yeast cell Anatomy 0.000 description 3
- 239000000203 mixture Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 244000285963 Kluyveromyces fragilis Species 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 238000003321 atomic absorption spectrophotometry Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 235000021048 nutrient requirements Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229910052761 rare earth metal Inorganic materials 0.000 description 1
- 150000002910 rare earth metals Chemical class 0.000 description 1
- 229940081969 saccharomyces cerevisiae Drugs 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention is the process of preparing chromium-rich yeast as chromium replenishing agent with live edible yeast and organic chromium compound. The preparation process includes the preparation of organic chromium mother liquid via dissolving nicotinic acid in water, adding glycine and chromium acetate while stirring and heating, regulating pH value until the liquid changes its color from green to royal purple and reaction for 1 hr in maintaining temperature and stirring; and the preparation of chromium-rich yeast via inoculating marketable live wine making yeast to the fermenting liquid, adding the organic chromium mother liquid, controlling pH 4.5-6.0 to ferment at 30-34 deg.c for 18-20 hr, centrifugal separation, water washing, drying, crushing, sieving and inspection. The chromium-rich yeast is used as one kind of chromium replenishing agent for various kinds of health food.
Description
Technical field
The present invention relates to food-yeast, specifically, relate to and be rich in trivalent chromium element zymic preparation method.
Background technology
As everyone knows, chromium is one of trace element of needed by human, and human body lacks chromium can make sugar tolerance impaired, and then it is slow to develop into diabetes, hyperlipidaemia, atherosclerosis, growth, and even shortens the life-span.And mend chromium above-mentioned illness is reversed.People lack the phenomenon ubiquity of chromium, and wherein insufficiency of intake is an important factor.Certainly, as heavy metal, the intake of chromium can not be excessive.The intake that the safety that U.S. nutrient requirement standard committee is recommended is suitable is μ g every days 50～200; Ministry of Health of China is used in the scale about nutrient prime replenisher, the minimum 50 μ g of consumption every day of chromium, the highest 150 μ g.Through research, people have found the supplement of some chromium, for example United States Patent (USP) 4343905 and No. 6248323, Chinese patent application No. 92104339.2, No. 98123177.2, No. 01102201.9, No. 02132569.3 etc. has all been announced the supplement and the manufacture method thereof of some chromium, and " being rich in the machine chromium yeast ", " chromium acetate yeast ", " Rich chromium yeast that contains high-biomass " and " rare earth chromium yeast " are wherein arranged.These supplement all are that people realize mending chromium by the dietary chromium yeast with the carrier of yeast as chromium.Yet, in the production technology of existing Rich chromium yeast, have plenty of the tervalent inorganic chromium of adding in substratum, it poisons bigger to yeast cell, be difficult for being absorbed formation GTF organic chromium by yeast cell; Though the technology that has is used the coordination compound of chromium, the structure of this compound does not determine that as yet preparation process is comparatively complicated.
Summary of the invention
Purpose of the present invention just provides and a kind ofly prepares the production technique of Rich chromium yeast by food-yeast and organic chromium, for people provide safe and effective chromium supplementing agent.
For achieving the above object, the contriver must solve two critical problems, and the one, zymic is selected, and the 2nd, the preparation of organic chromium.Wherein, yeast must have edible safety, easy cultivation, preservable performance; Organic chromium is to not only toxicological harmless of yeast cell, and wants and can easily be absorbed by yeast by chromium when fermentation, and is present among the yeast with the GTF state.
The contriver is through screening repeatedly and studying, select for use commercially available yeast saccharomyces cerevisiae (Saccharomycescerevisiae) as the base-material of cultivating Rich chromium yeast, this primary yeast in use among the people for many years, confirmed edible safety, easily cultivate, can buy easily from the market, also can in the certain hour scope, ensure the quality of products simultaneously; In the zymic nutrient solution, add definite glycine two nicotinic acid chromium of structure as the chromium source, its preparation method is simple, also can be from external import.
The production technique of Rich chromium yeast provided by the invention comprises:
(1) preparation organic chromium mother liquor: in water purification, add nicotinic acid, after the stirring and dissolving, 2: 1: 1 in molar ratio ratio adds glycine and chromium acetate, warming while stirring to 90 ℃, regulate pH value 4.5～6.0, question response liquid is hyacinthine by green transition, insulated and stirred reaction 1 hour, form the complex compound of chromium, be the organic chromium mother liquor that makes.
(2) preparation Rich chromium yeast: fermentation culture is sterilized, the cooling back feeds sterile air, insert active dry yeast, add the organic chromium mother liquor, control pH value 5.0～6.0, temperature reach after the stirring condition bottom fermentation is cultivated 18～20 hours for 30～34 ℃, centrifugation, discard liquid phase, wash solid phase with water, hot blast with 60 ℃ after cleaning carries out drying, crush and screen with ordinary method at last, the material of crossing 100 mesh sieves more after testing chromium content meet 〉=1000 μ g/g standards after, promptly make Rich chromium yeast.
In said process, used yeast is commercially available active yeast saccharomyces cerevisiae; Used fermentation culture is the nutrient solution that contains glycine two nicotinic acid chromium.
In said process, the concentration of the trivalent organic chromium in the fermented liquid is controlled at 500～600 μ g/ml.
The present invention produces Rich chromium yeast with commercially available active dry yeast as base-material, and it has edible safety, easy cultivation, preservable performance, and therefore can be used as raw material is used to prepare the nutritive health-care food that contains chromium; The glycine two nicotinic acid chromium preparation methods that join in the fermentation culture are simple; Chromium in the organic chromium complex compound of selecting for use can be effectively be absorbed by yeast when fermentation, and is present among the yeast with the GTF state.Containing trivalent organic chromium 〉=1000 μ g/g in the Rich chromium yeast with the inventive method production, is a kind of suitable chromium supplementing agent.Raw material of the present invention is easy to get, and technology is simple, be convenient to operation, amplify easily and form suitability for industrialized production.
Embodiment
Embodiment 1: the first step, organic chromium mother liquor preparation: in the 1700ml water purification, add nicotinic acid, after the stirring and dissolving, 2: 1: 1 in molar ratio ratio adds glycine and chromium acetate, and pH value 5.0～6.0 is regulated in warming while stirring to 90 ℃, after question response liquid is hyacinthine by green transition, insulated and stirred reaction 1 hour promptly makes organic chromium complex compound mother liquor, and is standby;
Second step, the preparation of Rich chromium yeast powder: get white sugar 520.0g, sal epsom 1.3g, primary ammonium phosphate 6.0g, water purification 14.0L joins in the fermentor tank of 50L, opens stirring, and 20min sterilizes under 100 ℃ of conditions of temperature; When being cooled to 30 ℃, feed sterile air, and access active dry yeast 1.0kg, add the standby sterilized organic chromium complex compound mother liquor 1.7L of back, control pH value 5.0～6.0, temperature reach for 30～34 ℃ ferments under agitation condition, through after 20 hours fermentation culture, liquid phase is removed in centrifugation; Wash solid phase with water, clean the back and carry out drying, crush and screen with ordinary method at last with 60 ℃ hot blast, the material of crossing 100 mesh sieves more after testing chromium content meet 〉=1000 μ g/g standards after, promptly make the about 0.5kg of Rich chromium yeast powder, pack and put in storage.
In the 3rd step, Rich chromium yeast and common brewer's dried yeasts that second step was prepared mix according to a certain percentage, prepare every 1g mixture and contain the about 200 μ g of trivalent organic chromium, incapsulate, and every capsules content is 0.3g.The name of this capsule product is called " day safe II capsule of peace board sugar ".
Embodiment 2: the Rich chromium yeast powder 5.0g that takes by weighing gained in the example 1 puts in the triangular flask of 100ml, adds distilled water 50ml, ultrasonic dissolution 15 minutes, leave standstill 15 minutes after, drain supernatant liquor; Add the ultrasonic vibration of 50ml water again after 15 minutes, drain supernatant liquor; Yeast cream is transferred in the centrifuge tube, after 3500-4000rpm is centrifugal 15 minutes, incline and supernatant liquor, merge all supernatant liquors, filter, filtrate decompression is concentrated into dried, with the content of atomic absorption spectrophotometry and determined by ultraviolet spectrophotometry chromium, the trivalent chromium of every gram Rich chromium yeast machinery absorption must not surpass 100～200 μ g, promptly is no more than 10%.
Claims (3)
1 one kinds prepare the production method of Rich chromium yeast by yeast and organic chromium, and its feature comprises:
(1) preparation organic chromium mother liquor: in water purification, add nicotinic acid, after the stirring and dissolving, 2: 1: 1 in molar ratio ratio adds glycine and chromium acetate solution, warming while stirring to 90 ℃, regulate pH value 5.5～6.0, question response liquid is hyacinthine by green transition, insulated and stirred reaction 1 hour, form the complex compound of chromium, promptly make the organic chromium mother liquor;
(2) preparation Rich chromium yeast: in the organic chromium mother liquor that makes, add commercially available active dry yeast and organic chromium mother liquor, feed sterile air, ferment under control pH value 4.5～6.0,30～34 ℃ of temperature and the agitation condition, through after 18～20 hours fermentation culture, centrifugation, remove liquid phase, wash solid phase with water, the hot blast with 60 ℃ after cleaning carries out drying, crushes and screens with ordinary method at last, the material of crossing 100 mesh sieves more after testing chromium content qualified after, promptly make Rich chromium yeast.
2 the method for claim 1 is characterized in that in said process, and used yeast is commercially available active yeast saccharomyces cerevisiae; Used fermentation culture is glycine two nicotinic acid chromium nutrient solutions.
3 the method for claim 1 is characterized in that the concentration of the trivalent organic chromium in the fermented liquid is controlled at 500～600 μ g/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031354912A CN1482236A (en) | 2003-07-25 | 2003-07-25 | Chrome-enriched yeast production method from edible yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031354912A CN1482236A (en) | 2003-07-25 | 2003-07-25 | Chrome-enriched yeast production method from edible yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1482236A true CN1482236A (en) | 2004-03-17 |
Family
ID=34154664
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031354912A Pending CN1482236A (en) | 2003-07-25 | 2003-07-25 | Chrome-enriched yeast production method from edible yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1482236A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100463961C (en) * | 2006-03-30 | 2009-02-25 | 安琪酵母股份有限公司 | Chromium-rich saccharomyces cerevisiae, chromium-rich yeast product and their production process |
| CN108236117A (en) * | 2017-10-13 | 2018-07-03 | 夏志君 | With yeast, its yeast protein after technology is processed can directly be eaten for food processing |
| WO2022143113A1 (en) * | 2020-12-30 | 2022-07-07 | 安琪酵母股份有限公司 | Chromium-rich yeast having high chromium absorption, preparation method therefor and application thereof |
-
2003
- 2003-07-25 CN CNA031354912A patent/CN1482236A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100463961C (en) * | 2006-03-30 | 2009-02-25 | 安琪酵母股份有限公司 | Chromium-rich saccharomyces cerevisiae, chromium-rich yeast product and their production process |
| CN108236117A (en) * | 2017-10-13 | 2018-07-03 | 夏志君 | With yeast, its yeast protein after technology is processed can directly be eaten for food processing |
| WO2022143113A1 (en) * | 2020-12-30 | 2022-07-07 | 安琪酵母股份有限公司 | Chromium-rich yeast having high chromium absorption, preparation method therefor and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101665523B (en) | The production method of L-arabinose and D-xylose pref | |
| CN106148445B (en) | A kind of new extraction technology of glutamic acid | |
| CN104212870B (en) | Process for fermentation production of lysine hydrochloride | |
| CN103082160B (en) | A kind of efficient deodorant, low nutrition ingredients from lossing breaking wall of spirullina princeps fishy-removing-method | |
| CN103343104B (en) | Process for preparing novel plant-source probiotics solution | |
| CN103305396B (en) | Method for producing cordyceps vinegar by use of cordyceps taishanensis fermentation liquor | |
| CN101570735A (en) | Method for producing flour yeast by using corn flour as raw material | |
| CN105602793A (en) | Preparation method of osmanthus wine | |
| CN104263793B (en) | Method for treating crystalline dextrose mother liquid | |
| CN104388317A (en) | A kind of organic chlorella production method rich in trace elements | |
| CN101665524B (en) | Method for producing L-arabinose | |
| CN101463316A (en) | Apple vinegar beverage | |
| CN105010761B (en) | A method of producing yeast fermentation state zinc selenium composite feed additive | |
| CN108112890B (en) | A kind of enzymatic hydrolysis and fermentation bone meal and preparation method thereof | |
| CN105053567B (en) | A method of utilizing beer waste liquid production yeast fermentation state copper feed additive | |
| CN101724531B (en) | Method for preparing rice puree spirit | |
| CN109294865A (en) | A kind of pure vinegar preparation method of envelope cylinder | |
| CN100535103C (en) | Method for preparing feedstuff yeast from maize peel hydrolysis sugar solution after extracting xylose | |
| CN109182000A (en) | A kind of Chinese chestnut rice wine and preparation method thereof | |
| CN1482236A (en) | Chrome-enriched yeast production method from edible yeast | |
| CN106244638B (en) | Comprehensive utilization process for producing lactic acid by biomass circulating fermentation | |
| CN104186938A (en) | Method for producing high-protein feed by using pumpkin leaves | |
| CN103265824B (en) | Production and refining method of gardenia red pigment | |
| CN109644778A (en) | A kind of edible fungus liquid fermentation medium and preparation method thereof | |
| CN113509427B (en) | Accurate control method for oat fermentation extract preparation process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |