CN100463961C - Chromium-rich saccharomyces cerevisiae, chromium-rich yeast product and their production process - Google Patents
Chromium-rich saccharomyces cerevisiae, chromium-rich yeast product and their production process Download PDFInfo
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Abstract
The present invention relates to one kind of chromium-rich Saccharomyces cerevisiae, chromium-rich Saccharomyces cerevisiae product and their production process. Of the chromium-rich Saccharomyces cerevisiae product, each kilogram contains chromium in 4000-8000 mg, and organochromium accounts for 90-99 wt% of the total chromium content. The process of producing chromium-rich Saccharomyces cerevisiae product includes the following steps: 1. fermenting the chromium-rich Saccharomyces cerevisiae of the present invention in culture medium containing chromium in 10-300 mcg/ml at 26-35 deg.c; and 2. drying the fermented Saccharomyces cerevisiae liquid to obtain the chromium-rich Saccharomyces cerevisiae product. The chromium-rich Saccharomyces cerevisiae product is safe and non-toxic, and may be used in medicine, food, feed and other fields.
Description
Technical field
The present invention relates to a kind of rich chromium yeast saccharomyces cerevisiae, derive from the Rich chromium yeast product and the production method thereof of this richness chromium yeast saccharomyces cerevisiae.
Background technology
Chromium is the indispensable a kind of trace element of human body and animal, has participated in the sugar metabolism and the metabolism of fat of body, has a lot of important physical functions.Scarce chromium can cause diseases such as diabetes and arteriosclerosis.Cataract, hyperlipidemia etc. are also relevant with long-term scarce chromium.Although human body is very little to the demand of chromium, the intravital scarce chromium phenomenon of modern is ubiquity still, this is the minimizing that causes intake because can run off a large amount of chromium in food precision work process, refining on the other hand processed food also can promote to retain in the body a large amount of drainages of chromium, makes that lacking chromium further aggravates.Epidemiology survey shows that the intake of chromium usually can be lower than demand, particularly diabetics and the elderly in modern's normal diet all needs additionally to mend chromium.Rich chromium yeast is obvious for replenishing the some diseases effect that causes because of scarce chromium, and very rich nutrient contents is arranged in the yeast in addition, can bring into play synergy.
Many to Rich chromium yeast research both at home and abroad in recent years, but seldom can produce greatly, main problem is: (1) is not optimized chromium ion concentration, the culture condition of the substratum of cultivating Rich chromium yeast, or optimize not enough, make the chromium content in the yeast lower, generally below 1000mg/kg, the highlyest also can only reach 4000mg/kg; (2) organic chromium content is low, and it is simple yeast and chromium absorption or mixing that part is arranged, and inorganic chromium is not converted into organic chromium; (3) certain methods is not suitable for big production, and repeatability is very poor, and some can be produced, but productive rate is very low; (4) utilization ratio to chromium is low.
Summary of the invention
The object of the invention is to provide a kind of Rich chromium yeast product and production method thereof.Utilize GENERALIZATION OF MODERN BREEDING TECHNIQUE, by screening the barms that obtains the biomass height and inorganic chromium is had tolerance, and then by the chemomorphosis technology, select chromium content height, growth velocity is fast, productive rate is high, to the Rich chromium yeast bacterial classification of the high anti-Gao Ge of the utilization ratio of chromium.By the zymotechnique of optimization, obtain the Rich chromium yeast product of high chromium content again, chromium content is 4000～8000mg/kg.The Rich chromium yeast product of high chromium content is divided into to be had activity and not to have active two kinds.
Of the present invention aspect first in, a kind of rich chromium yeast saccharomyces cerevisiae Z1.3 (Saccharomycescerevisiae Hansen Z1.3) is provided, its on October 25th, 2005 at China typical culture collection center (CCTCC, Wuhan University, postcode 430072) carried out biological preservation, deposit number is CCTCC M 205125.It is to come out containing on the substratum of chromium seed selection by nitrosoguanidine mutagenesis.
In aspect second of the present invention, provide a kind of Rich chromium yeast product, it derives from the rich chromium yeast saccharomyces cerevisiae of above-mentioned the present invention, contains chromium 4000～8000mg in wherein every kg Rich chromium yeast product, and wherein organic chromium content accounts for 90～99 weight % of total chromium.
In a preferred embodiment aspect this, contain chromium 4000～6000mg in every kg Rich chromium yeast product of the present invention.
In a further preferred embodiment, organic chromium content accounts for the 93-99 weight % of total chromium in the Rich chromium yeast product of the present invention.
In bright another preferred embodiment of we, the living yeast rate in the Rich chromium yeast product of the present invention is 80～95%.
In aspect the 3rd of the present invention, provide a kind of method of production chromium-rich Saccharomyces cerevisiae product, comprised the following steps:
(a) make rich chromium yeast saccharomyces cerevisiae of the present invention in the substratum that contains chromium 10～300 μ g/ml, under 26～35 ℃ temperature, ferment;
(b), promptly get the Rich chromium yeast product with the yeast fermentation broth drying.
In a preferred embodiment of the inventive method, chromium content is 100 μ g/ml in the wherein said substratum.
In another preferred embodiment of the inventive method, wherein said leavening temperature is 30 ℃.
In another preferred embodiment of the inventive method, wherein said drying is with yeast fermentation broth dust drying or vapor pressure roller drying.
In another preferred embodiment of the inventive method, wherein said drying is that yeast fermentation broth is carried out lyophilize.
Principal feature of the present invention has:
(1) the anti-chromium stable performance of Rich chromium yeast bacterial classification of the anti-Gao Ge of the present invention's use, growth velocity is fast, to the utilization ratio height of chromium, contains the chromium height and can reach 4000～8000mg/kg.The Rich chromium yeast chromium content that former method and technology is produced below 1000mg/kg, the highest 4000mg/kg that just arrives.
(2) the present invention's Rich chromium yeast of producing high chromium content can have activity, and the Rich chromium yeast that former technology is produced is not have active Rich chromium yeast mostly.
(3) the Rich chromium yeast organic chromium content height of the high chromium content of the present invention's production can reach 99%.
Embodiment
Of the present invention aspect first in, a kind of rich chromium yeast saccharomyces cerevisiae Z1.3 (Saccharomycescerevisiae Hansen Z1.3) is provided, its on October 25th, 2005 at China typical culture collection center (CCTCC, Wuhan University, postcode 430072) carried out biological preservation, deposit number is CCTCC M205125.It is to come out containing on the substratum of chromium seed selection by lithium chloride mutagenesis.
Inorganic chromium toxicity is bigger, general yeast is difficult in the condition that contains chromium and is growing, even growth velocity and also not high to the utilization ratio of chromium is arranged, the present invention is by lithium chloride mutagenesis, select chromium content height, growth velocity is fast, productive rate is high, to the Rich chromium yeast bacterial classification C460 of the high anti-Gao Ge of the utilization ratio of chromium.Concrete implementation step is as follows:
1) barms of the anti-Gao Ge of screening: the barms in different purposes and source is inoculated into containing in the chromium substratum of different concns, the bacterial classification that screening can be grown in high chromium substratum.Pick out the bacterial classification of the bacterial classification of anti-chromium as mutagenesis.
2) bacterial classification inoculation with anti-chromium carries out mutagenesis to the substratum of the lithium chloride that contains various dose.
3) yeast strain with mutagenesis is carrying out separation screening, utilization shake the bottle and the small-sized fermentation jar ferment select contain chromium content height on the substratum of chromium, growth velocity is fast, productive rate is high, to the Rich chromium yeast bacterial classification of the high anti-Gao Ge of the utilization ratio of chromium, be the Rich chromium yeast bacterial classification of anti-Gao Ge.
4) screening and culturing based formulas
Malt extract medium sugar degree 5～25 weight %, containing chromium (chromium trichloride) concentration is 10～300 μ g/ml, the pH value is 4～6.5.
5) screening and culturing condition
26～35 ℃ of temperature, stir culture 18～48 hours.
In aspect second of the present invention, provide a kind of Rich chromium yeast product, it derives from above-mentioned rich chromium yeast saccharomyces cerevisiae, contains chromium 4000～8000mg in wherein every kg Rich chromium yeast product, preferably contains chromium 4000～6000mg; Wherein organic chromium content accounts for 90～99 weight % of total chromium, preferably accounts for the 93-99 weight % of total chromium; Also preferred living yeast rate (the living yeast number accounts for the per-cent of total cell number) is calculated as 80～95% through flat board dyeing.
In aspect the 3rd of the present invention, the method of production chromium-rich Saccharomyces cerevisiae product is provided, and its Production Flow Chart is as follows: slant strains cultivation → level liquid seed culture → secondary liquid seeds cultivation → fermentor tank seed culture → fermentor cultivation → separation → drying → packing.
1) slant strains is cultivated: is that 5～25 weight %, chromium (chromium trichloride) concentration are that 10～300 μ g/ml, pH value are on 4～6.5 the wort solid inclined-plane, 26～35 ℃ of cultivations 24～48 hours with the Rich chromium yeast bacterial classification inoculation of anti-Gao Ge to sugar degree.
2) level liquid seed culture: it is that 5～25 weight %, chromium concn are that 10～300 μ g/ml, pH value are in 4～6.5 the special-purpose culturing bottle of wort, 26～35 ℃ of cultivations 24～48 hours that the barms on inclined-plane inoculation 1～4 is encircled to the sugar degree that 100～500ml is housed.
3) the secondary liquid seeds is cultivated; It is that 5～25 weight %, chromium (chromium trichloride) concentration are that 10～300 μ g/ml, pH value are in 4～6.5 the Ka Shi culturing bottle, 26～35 ℃ of cultivations 18～48 hours that the level liquid seed culture fluid is inoculated into sugar (mixtures of wort or molasses or wort and the molasses) amount of containing that 10～40L is housed.
4) fermentor tank seed culture: the secondary seed nutrient solution is inoculated into 0.5～15m is housed
3Sugar (mixtures of wort or molasses or wort and the molasses) amount of containing be that 5～25 weight %, chromium (chromium trichloride) concentration are 10～300 μ g/ml, to replenish suitable nitrogenous source and phosphorus source, pH value be in 4～6.5 the seed fermentation jar, 26～35 ℃ of cultivations 18～48 hours.
5) ferment tank is cultivated: it is that 5～25 weight %, chromium (chromium trichloride) concentration are 10～300 μ g/ml, to replenish suitable nitrogenous source and phosphorus source, pH value be in 4～6.5 the fermentor tank that the fermentor tank seed culture fluid is inoculated into 1～300 ton sugar (mixtures of amylum hydrolysate of the sugar or molasses or amylum hydrolysate of the sugar and the molasses) amount that contains, used nutrition source adopts fed-batch mode, chromium adds in batches, cultivates 14～36 hours at 26～35 ℃.
6) separate: adopt stacked separating machine to separate again and filter or use filter press with vacuum drum.
7) drying: adopt 60～140 ℃ of fluidized dryings, the exsiccant product has activity; Adopt 100～180 ℃ of dryings or with the vapor pressure roller drying of 3～6Kpa, it is powdery or strip that drying products does not have activity of dusting; Also can adopt the freeze-drying drying.
8) packing: can adopt the packing of various ways to become the Rich chromium yeast of high chromium content.
Need to add an amount of nitrogenous source and phosphorus source in fermentor tank seed culture and ferment tank cultivation, nitrogenous source is selected from sulfate of ammoniac, bicarbonate of ammonia, ammoniacal liquor, phosphoric acid ammonia, phosphoric acid hydrogen ammonia and DAP, or the combination of these materials.The phosphorus source is for being selected from phosphoric acid, phosphoric acid ammonia, phosphoric acid hydrogen ammonia and DAP, or the combination of these materials.
If successive production can increase the fermentation of one grade fermemtation jar secondary seed, fermentation condition is: the fermentor tank seed culture fluid is inoculated into that 1～300 ton sugar (mixtures of amylum hydrolysate of the sugar or molasses or amylum hydrolysate of the sugar and the molasses) amount of containing is 5～25%, chromium (chromium trichloride) concentration is 10～300 μ g/ml, replenishes suitable nitrogenous source and phosphorus source, pH value is in 4～6.5 the secondary seed fermentor tank, used nutrition source adopts fed-batch mode, chromium adds in batches, cultivates 14～36 hours at 26～35 ℃.Carry out whizzer or sheet frame then and separate, in 4～10 ℃ of storage tanks or freezer, preserve, be inoculated into as required then and carry out the next stage cultivation in the fermentor tank again.
Can produce Rich chromium yeast product of the present invention by aforesaid method, contain chromium 4000～8000mg in wherein every kg Rich chromium yeast product, preferably contain chromium 4000～6000mg; Wherein organic chromium content accounts for 90～99 weight % of total chromium, preferably accounts for the 93-99 weight % of total chromium; Also preferred living yeast rate is calculated as 80～95% through flat board dyeing.
To describe feature of the present invention and all respects in detail by specific embodiment below.Unless specialize, experimental technique and reagent used among the present invention are method known in those skilled in the art and reagent.In addition, embodiment is interpreted as illustrative, rather than will limit the scope of the invention, and the spirit and scope of the invention only are defined by the claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes or change that the nutrient media components in these embodiments, content, culture condition, separation processing conditions are carried out also belong to protection scope of the present invention.
Embodiment 1: the screening of yeast saccharomyces cerevisiae bacterial classification of the present invention
1) barms different with purposes of will originating is inoculated into containing in the chromium substratum of different concns, the bacterial classification that screening can be grown in high chromium substratum.Pick out the bacterial classification of the bacterial classification of anti-chromium as mutagenesis.
2) bacterial classification inoculation with anti-chromium carried out mutagenesis 24 hours to the substratum of the lithium chloride that contains various dose.
3) yeast strain with mutagenesis is carrying out separation screening, utilization shake the bottle and the small-sized fermentation jar ferment select contain chromium content height on the substratum of chromium, growth velocity is fast, productive rate is high, to the Rich chromium yeast bacterial classification of the high anti-Gao Ge of the utilization ratio of chromium, be the Rich chromium yeast bacterial classification C460 of anti-Gao Ge.
4) screening and culturing based formulas
Malt extract medium sugar degree 5～25 weight % contain chromium (chromium trichloride) amount 10～300 μ g/ml, and the pH value is 4～6.5.
5) screening and culturing condition
26～35 ℃ of temperature, stir culture 18～48 hours.
Embodiment 2: the production-scheme 1 of Rich chromium yeast product
Produce the Production Flow Chart of high chromium content Rich chromium yeast: slant strains cultivation → level liquid seed culture → secondary liquid seeds cultivation → fermentor tank seed culture → fermentor cultivation → separation → drying → packing.Concrete technology is as follows:
1) slant strains is cultivated: is that 5～25 weight %, chromium (chromium trichloride) concentration are that 10 μ g/ml, pH value are on 4～6.5 the wort solid inclined-plane with the Rich chromium yeast bacterial classification inoculation of anti-Gao Ge to sugar degree, at 26～35 ℃, cultivates 24～48 hours.
2) level liquid seed culture: it is that 5～25 weight %, chromium (chromium trichloride) concentration are that 10～300 μ g/ml, pH value are in 4～6.5 the special-purpose culturing bottle of wort, 26～35 ℃ of cultivations 24～48 hours that the barms on inclined-plane inoculation 1～4 is encircled to the sugar degree that 100～500ml is housed.
3) the secondary liquid seeds is cultivated; It is that 5～25 weight %, chromium (chromium trichloride) concentration are that 10 μ g/ml, pH value are in 4～6.5 the Ka Shi culturing bottle, 26～35 ℃ of cultivations 18～48 hours that the level liquid seed culture fluid is inoculated into sugar (mixtures of wort or molasses or wort and the molasses) amount of containing that 10～40L is housed.
4) fermentor tank seed culture: the secondary seed nutrient solution is inoculated into 0.5～15m is housed
3Sugar (mixtures of wort or molasses or wort and the molasses) amount of containing be that 5～25 weight %, chromium (chromium trichloride) concentration are 10 μ g/ml, add 1～5 weight % phosphoric acid hydrogen ammonia, pH value and be in 4～6.5 the seed fermentation jar, cultivated 18～48 hours at 26～35 ℃.
5) ferment tank is cultivated: with the fermentor tank seed culture fluid be inoculated into that 1～300 ton sugar (mixtures of amylum hydrolysate of the sugar or molasses or amylum hydrolysate of the sugar and the molasses) amount that contains is 5～25%, chromium (chromium trichloride) concentration is 10 μ g/ml, add 1～5 weight % phosphoric acid hydrogen ammonia, the pH value is in 4～6.5 the fermentor tank, used nutrition source adopts fed-batch mode, chromium adds in batches, cultivates 14～36 hours at 26～35 ℃.
6) separate: adopt stacked separating machine to separate again and filter or use filter press with vacuum drum.
7) drying: adopt 60～140 ℃ of fluidized dryings, the exsiccant product has activity; Adopt 100～180 ℃ of dryings or with the vapor pressure roller drying of 3～6Kpa, it is powdery or strip that drying products does not have activity of dusting; Also can adopt the freeze-drying drying.
8) packing: can adopt the packing of various ways to become the Rich chromium yeast of high chromium content.Chromium content reaches 4200mg/kg.
Embodiment 3: the production-scheme 2 of Rich chromium yeast product
Produce the Production Flow Chart of high chromium content Rich chromium yeast: slant strains cultivation → level liquid seed culture → secondary liquid seeds cultivation → fermentor tank seed culture → fermentor cultivation → separation → drying → packing.Concrete technology is as follows:
1) slant strains is cultivated: is that 5～25 weight %, chromium (chromium trichloride) concentration are that 300 μ g/ml, pH value are on 4～6.5 the wort solid inclined-plane with the Rich chromium yeast bacterial classification inoculation of anti-Gao Ge to sugar degree, at 26～35 ℃, cultivates 24～48 hours.
2) level liquid seed culture: it is that 5～25 weight %, chromium (chromium trichloride) concentration are that 300 μ g/ml, pH value are in 4～6.5 the special-purpose culturing bottle of wort, 26～35 ℃ of cultivations 24～48 hours that the barms on inclined-plane inoculation 1～4 is encircled to the sugar degree that 100～500ml is housed.
3) the secondary liquid seeds is cultivated; It is that 5～25 weight %, chromium (chromium trichloride) concentration are that 300 μ g/ml, pH value are in 4～6.5 the Ka Shi culturing bottle, 26～35 ℃ of cultivations 18～48 hours that the level liquid seed culture fluid is inoculated into sugar (mixtures of wort or molasses or wort and the molasses) amount of containing that 10～40L is housed.
4) fermentor tank seed culture: the secondary seed nutrient solution is inoculated into 0.5～15m is housed
3Sugar (mixtures of wort or molasses or wort and the molasses) amount of containing be that 5～25 weight %, chromium (chromium trichloride) concentration are 300 μ g/ml, add 1～5 weight % phosphoric acid hydrogen ammonia, pH value and be in 4～6.5 the seed fermentation jar, cultivated 18～48 hours at 26～35 ℃.
5) ferment tank is cultivated: it is that 5～25 weight %, chromium (chromium trichloride) concentration are 300 μ g/ml, to add 1～5 weight % phosphoric acid hydrogen ammonia, pH value be in 4～6.5 the fermentor tank that the fermentor tank seed culture fluid is inoculated into 1～300 ton sugar (mixtures of amylum hydrolysate of the sugar or molasses or amylum hydrolysate of the sugar and the molasses) amount that contains, used nutrition source adopts fed-batch mode, chromium adds in batches, cultivates 14～36 hours at 26～35 ℃.
6) separate: adopt stacked separating machine to separate again and filter or use filter press with vacuum drum.
7) drying: adopt 60～140 ℃ of fluidized dryings, the exsiccant product has activity; Adopt 100～180 ℃ of dryings or with the vapor pressure roller drying of 3～6Kpa, it is powdery or strip that drying products does not have activity of dusting; Also can adopt the freeze-drying drying.
8) packing: can adopt the packing of various ways to become the Rich chromium yeast of high chromium content.Chromium content reaches 8000mg/kg.
Embodiment 4: the production-scheme 3 of Rich chromium yeast product
Produce the Production Flow Chart of high chromium content Rich chromium yeast: slant strains cultivation → level liquid seed culture → secondary liquid seeds cultivation → fermentor tank seed culture → fermentor cultivation → separation → drying → packing.Concrete technology is as follows:
1) slant strains is cultivated: is that 5～25 weight %, chromium (chromium trichloride) concentration are that 100 μ g/ml, pH value are on 4～6.5 the wort solid inclined-plane with the Rich chromium yeast bacterial classification inoculation of anti-Gao Ge to sugar degree, at 26～35 ℃, cultivates 24～48 hours.
2) level liquid seed culture: it is that 5～25 weight %, chromium (chromium trichloride) concentration are that 100 μ g/ml, pH value are in 4～6.5 the special-purpose culturing bottle of wort, 26～35 ℃ of cultivations 24～48 hours that the barms on inclined-plane inoculation 1～4 is encircled to the sugar degree that 100～500ml is housed.
3) the secondary liquid seeds is cultivated; It is that 5～25 weight %, chromium (chromium trichloride) concentration are that 100 μ g/ml, pH value are in 4～6.5 the Ka Shi culturing bottle, 26～35 ℃ of cultivations 18～48 hours that the level liquid seed culture fluid is inoculated into sugar (mixtures of wort or molasses or wort and the molasses) amount of containing that 10～40L is housed.
4) fermentor tank seed culture: the secondary seed nutrient solution is inoculated into 0.5～15m is housed
3Sugar (mixtures of wort or molasses or wort and the molasses) amount of containing be that 5～25 weight %, chromium (chromium trichloride) concentration are 100 μ g/ml, add 1～5% phosphoric acid hydrogen ammonia, pH value and be in 4～6.5 the seed fermentation jar, cultivated 18～48 hours at 26～35 ℃.
5) ferment tank is cultivated: it is that 5～25 weight %, chromium (chromium trichloride) concentration are 100 μ g/ml, to add 1～5 weight % phosphoric acid hydrogen ammonia, pH value be in 4～6.5 the fermentor tank that the fermentor tank seed culture fluid is inoculated into 1～300 ton sugar (mixtures of amylum hydrolysate of the sugar or molasses or amylum hydrolysate of the sugar and the molasses) amount that contains, used nutrition source adopts fed-batch mode, chromium adds in batches, cultivates 14～36 hours at 26～35 ℃.
6) separate: adopt stacked separating machine to separate again and filter or use filter press with vacuum drum.
7) drying: adopt 60～140 ℃ of fluidized dryings, the exsiccant product has activity; Adopt 100～180 ℃ of dryings or with the vapor pressure roller drying of 3～6Kpa, it is powdery or strip that drying products does not have activity of dusting; Also can adopt the freeze-drying drying.
8) packing: can adopt the packing of various ways to become the Rich chromium yeast of high chromium content.Chromium content reaches 6500mg/kg.
Embodiment 5: the production-scheme 4 of Rich chromium yeast product
Produce the Production Flow Chart of high chromium content Rich chromium yeast: slant strains cultivation → level liquid seed culture → secondary liquid seeds cultivation → fermentor tank seed culture → fermentor cultivation → separation → drying → packing.Concrete technology is as follows:
1) slant strains is cultivated: is that 5～25 weight %, chromium (chromium trichloride) concentration are that 200 μ g/ml, pH value are on 4～6.5 the wort solid inclined-plane with the Rich chromium yeast bacterial classification inoculation of anti-Gao Ge to sugar degree, at 26～35 ℃, cultivates 24～48 hours.
2) level liquid seed culture: it is that 5～25 weight %, chromium (chromium trichloride) concentration are that 200 μ g/ml, pH value are in 4～6.5 the special-purpose culturing bottle of wort, 26～35 ℃ of cultivations 24～48 hours that the barms on inclined-plane inoculation 1～4 is encircled to the sugar degree that 100～500ml is housed.
3) the secondary liquid seeds is cultivated; It is that 5～25 weight %, chromium (chromium trichloride) concentration are that 200 μ g/ml, pH value are in 4～6.5 the Ka Shi culturing bottle, 26～35 ℃ of cultivations 18～48 hours that the level liquid seed culture fluid is inoculated into sugar (mixtures of wort or molasses or wort and the molasses) amount of containing that 10～40L is housed.
4) fermentor tank seed culture: the secondary seed nutrient solution is inoculated into 0.5～15m is housed
3Sugar (mixtures of wort or molasses or wort and the molasses) amount of containing be that 5～25 weight %, chromium (chromium trichloride) concentration are 200 μ g/ml, add 1～5 weight % phosphoric acid hydrogen ammonia, pH value and be in 4～6.5 the seed fermentation jar, cultivated 18～48 hours at 26～35 ℃.
5) ferment tank is cultivated: it is that 5～25 weight %, chromium (chromium trichloride) concentration are 200 μ g/ml, to add 1～5 weight % phosphoric acid hydrogen ammonia, pH value be in 4～6.5 the fermentor tank that the fermentor tank seed culture fluid is inoculated into 1～300 ton sugar (mixtures of amylum hydrolysate of the sugar or molasses or amylum hydrolysate of the sugar and the molasses) amount that contains, used nutrition source adopts fed-batch mode, chromium adds in batches, cultivates 14～36 hours at 26～35 ℃.
6) separate: adopt stacked separating machine to separate again and filter or use filter press with vacuum drum.
7) drying: adopt 60～140 ℃ of fluidized dryings, the exsiccant product has activity; Adopt 100～180 ℃ of dryings or with the vapor pressure roller drying of 3～6Kpa, it is powdery or strip that drying products does not have activity of dusting; Also can adopt the freeze-drying drying.
8) packing: can adopt the packing of various ways to become the Rich chromium yeast of high chromium content.Chromium content reaches 7100mg/kg.
Embodiment 6: the production-scheme 5 of Rich chromium yeast product
Present embodiment increases one grade fermemtation jar seed culture, and production technique is: slant strains cultivation → level liquid seed culture → secondary liquid seeds cultivation → one grade fermemtation jar seed culture → second order fermentation jar seed culture → fermentor cultivation → separation → drying → packing.The one grade fermemtation jar seed culture method and the fermentor cultivation that are increased are roughly the same, other technologies are with embodiment 2, just ferment tank is cultivated and is selected amylum hydrolysate of the sugar 8 weight % and molasses 5 weight % to do that carbon source, ammoniacal liquor 3 weight % are made nitrogenous source, phosphoric acid hydrogen ammonia 2 weight % do the phosphorus source, chromium content in the nutrient solution is 100 μ g/ml, the pH value is 5.2, and 28～32 ℃ of air blast of temperature were cultivated 26 hours.
The Rich chromium yeast chromium content of the high chromium content that present embodiment is produced reaches 5500mg/kg.
Embodiment 7: the production-scheme 6 of Rich chromium yeast product
Present embodiment adopts fluidized exsiccant method drying to provide the Rich chromium yeast of activated high chromium content, and drying process is: 120～140 ℃ of inlet temperature, and 70～100 ℃ of air outlet temperatures, dry 20～50 minutes, moisture was less than 7 weight %.
Production technique:
Slant strains cultivation → level liquid seed culture → secondary liquid seeds cultivation → fermentor tank seed culture fermentor cultivation → separation → drying → packing.
The living cell rate of the Rich chromium yeast of the high chromium content that present embodiment is produced reaches more than 80%.Other technologies are with embodiment 2.
In sum, the Microbial Genetical Breeding technology that takes full advantage of of the present invention, microbial fermentation technology is according to yeast Bacterium filters out the barms of anti-Gao Ge to the characteristic of chromium tolerance, again by induced-mutation technique select chromium content height, Growth rate is fast, productive rate is high, to the Cr-enriched yeast bacterial classification of the high anti-Gao Ge of the utilization rate of chromium. Formulate for this bacterial classification Special process produce the Cr-enriched yeast of high chromium content, characteristics are: anti-chromium stable performance, growth rate is fast, and is right The utilization rate height of chromium; Organic Chromium content height can reach 99%; The Cr-enriched yeast of this high chromium content can be active There is not activity yet. Has more wide application space.
Claims (8)
1. a rich chromium yeast saccharomyces cerevisiae Z1.3 (Saccharomyces cerevisiae Hansen Z1.3), its deposit number is CCTCC M 205125.
2. Rich chromium yeast product, it derives from the described rich chromium yeast saccharomyces cerevisiae of claim 1, contains chromium 4000～8000mg in wherein every kg Rich chromium yeast product.
3. the described Rich chromium yeast product of claim 2 contains chromium 4000～6000mg in wherein every kg Rich chromium yeast product.
4. according to claim 2 or 3 described Rich chromium yeast products, wherein the living yeast rate is 80～95%.
5. a method of producing the described Rich chromium yeast product of claim 2 comprises the following steps:
(a) make the described rich chromium yeast saccharomyces cerevisiae of claim 1 in the substratum that contains chromium 10～300 μ g/ml, under 26～35 ℃ temperature, ferment;
(b), promptly get the Rich chromium yeast product with the yeast fermentation broth drying.
6. the described method of claim 5, chromium content is 100 μ g/ml in the wherein said substratum.
7. the described method of claim 5, wherein said leavening temperature is 30 ℃.
8. any described method among the claim 5-7, wherein said drying are with yeast fermentation broth dust drying, vapor pressure roller drying or lyophilize.
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| CNB200610066365XA CN100463961C (en) | 2006-03-30 | 2006-03-30 | Chromium-rich saccharomyces cerevisiae, chromium-rich yeast product and their production process |
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| CNB200610066365XA CN100463961C (en) | 2006-03-30 | 2006-03-30 | Chromium-rich saccharomyces cerevisiae, chromium-rich yeast product and their production process |
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| CN100463961C true CN100463961C (en) | 2009-02-25 |
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| CN103396955B (en) * | 2013-07-15 | 2015-03-04 | 浙江深友生物技术有限公司 | Yeast and method for producing chromium-enriched yeast by utilizing yeast via high-density fermentation |
| CN106608908B (en) * | 2015-10-22 | 2020-04-14 | 中国农业科学院农产品加工研究所 | Method for extracting glucose tolerance factor from chromium-containing yeast |
| CN105861347B (en) * | 2016-05-24 | 2019-08-30 | 北京龙科方舟生物工程技术有限公司 | A method of yeast chromium is produced using nanometer chromium |
| CN113025510B (en) * | 2019-12-25 | 2022-09-16 | 中国科学院微生物研究所 | Function-enhanced yeast culture rich in organic trace elements and preparation method thereof |
| CN114686383B (en) * | 2020-12-30 | 2023-12-19 | 安琪酵母股份有限公司 | Chromium-enriched yeast with high chromium absorptivity, and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58220684A (en) * | 1982-06-07 | 1983-12-22 | ユニヴア−サル・フツヅ・コ−ポレ−シヨン | Production of chromium yeast |
| CN1366037A (en) * | 2001-01-17 | 2002-08-28 | 中国科学院微生物研究所 | Cr-enriched yeast containing high biomass and its preparing process |
| CN1482236A (en) * | 2003-07-25 | 2004-03-17 | 贵州天安药业股份有限公司 | Chrome-enriched yeast production method from edible yeast |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58220684A (en) * | 1982-06-07 | 1983-12-22 | ユニヴア−サル・フツヅ・コ−ポレ−シヨン | Production of chromium yeast |
| CN1366037A (en) * | 2001-01-17 | 2002-08-28 | 中国科学院微生物研究所 | Cr-enriched yeast containing high biomass and its preparing process |
| CN1482236A (en) * | 2003-07-25 | 2004-03-17 | 贵州天安药业股份有限公司 | Chrome-enriched yeast production method from edible yeast |
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