CN1477102A - Sugarapple plant extract with action of resisting diabetes, medicinal application and preparation method - Google Patents
Sugarapple plant extract with action of resisting diabetes, medicinal application and preparation method Download PDFInfo
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- CN1477102A CN1477102A CNA031115217A CN03111521A CN1477102A CN 1477102 A CN1477102 A CN 1477102A CN A031115217 A CNA031115217 A CN A031115217A CN 03111521 A CN03111521 A CN 03111521A CN 1477102 A CN1477102 A CN 1477102A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The present invention relates to a plant extract with the action of resisting diabetes, its application and preparation method. The extract is a kind of acetogenins extracted from branck, leaf, trunk, bark, root, seed and fruit skin of annona squamosa plant as raw material. The compound acetogenins can be made into various dosage forms for curing diabetes, and its preparation method can adopt one or several processes of solvent extraction process, resin adsorption process, supercritical CO2 extraction process and conventional drying process.
Description
Technical field:
The present invention relates to medical technical field, exactly it is a kind of plant milk extract of antidiabetic effect---annonaceae plant milk extract and medicinal use and preparation method.
Background technology:
Diabetes have become common disease, the frequently-occurring disease that threatens human health.According to the 5th IDF's Xi Tai district meeting holding in Beijing, the global at present type ii diabetes of having diagnosed reaches 1.3 hundred million people, and China has surpassed 40,000,000 people; Type ii diabetes will be popular in developing countries such as China, India 21 century, and by 2005, global diabetic subject will break through 300,000,000, and China diabetic subject sum is near 100,000,000, become second big country that diabetic subject's number in the world is only second to India.China's flower in 1994 approximately is 41,900,000,000 yuans in the medical expense of diabetes and cardiovascular diseases, increases sharply by 2000 to 1,216 hundred million yuan [dawn, China's Chinese materia medica was reported 2002-09-23].The medicine of present western medical treatment diabetes has a variety of, still, up to now, does not also have to find thoroughly to cure the medicine of diabetes.
The Chinese medicine diabetes have history in several thousand, have accumulated rich experience, have formed the theoretical system and the methods of treatment of system.Also accumulating rich experience (Wang Mingxia, Chinese medicine diabetic complication recent developments aspect the treatment diabetic complication.Beijing University of Chinese Medicine's journal, 1996,19 (5): 43-44), (Jiang Lijuan, Li Li.Active ingredient of Chinese herbs treatment diabetic complication Mechanism Study progress, 63), (Cui Yunzhu, Cui Yong Anhui Chinese Medicine College journal, 2001,20 (4):.Chinese medicine is to the clinical investigation of chronic complicating diseases of diabetes preventive and therapeutic effect, and Shandong Traditional Chinese Medicine University's journal 2002,26 (1): 44-46), the ARI that seeks high-efficiency low-toxicity from natural product also is a research emphasis in recent years always.Mexican opuntia is traditional hypoglycemic plant amedica.Do medicinal opuntia at home and be mainly Root and stem of Cholla (Opuntiadillenii Haw) and Yunnan Root and stem of Cholla (Opuntia monocantha Haw).About the Root and stem of Cholla Study on plants remarkable progress is arranged also, by retrieval about patent and document, at present both at home and abroad certain progress is arranged, mainly concentrate on hypoglycemic, antiulcer action, antibacterial, relieving inflammation and relaxing pain, the anti-ageing effect of waiting for a long time (Li Xiangzhong, Zhang Ruhua about the research that utilizes Root and stem of Cholla treatment diabetes.Cactus preparation and preparation method thereof application number 93110151.4 publication numbers 1076623), (a kind of cactus granule preparation of Zhao Xiang and preparation method thereof application number 98122873.9 publication numbers 1260183), (Zhang Wangui cactus plant health-care beverage application number 99103253.5 publication numbers 1268324), (manufacture method application number 99103254.3 publication numbers 1268318 of Zhang Wangui cactus plant health-care beverage), (Yu Dongping, Li Ping, Gao Dongshun, Yu Jing.Health product of cactus and working method application number 00100604.5 publication number 1306834 thereof), (Qiu Yingkun, Chen Yingjie.The Opuntia Research progress in medicinal, Chinese pharmaceutical chemistry magazine, 1999,9 (3): 223-227), (He Xiangjiu, Yao new life.Opuntia chemical ingredients and Advance on Pharmacological Activities.Research and development of natural products, 2000,06), (Wang Zhao, Lin Lin, Bao Shiquan.Composition analysis and the DEVELOPMENT PROSPECT of China Hainan Root and stem of Cholla.Research and development of natural products, 2001,01), (Lin Lin, Bao Shiquan, Wang Zhao.The active skull cap components research of Root and stem of Cholla is looked back.GUIHAIA, 2002,22 (4): 375-381), (Lee is to middle Zhang Ruhua benefit Tianjin antihypelipidemic preparation and preparation method thereof application number 93110152), (old) towards the blue Root and stem of Cholla fruit juice of silver-colored Zhao Sheng, preserved fruit, beverage and manufacture method application number 94116748 thereof.
Annonaceae (Annona Linn.) plant also is a kind of natural phant, comprise that Asimina, two calyxs belong to, the brother receive fragrantly belong to, the eyebrow lobe belongs to, volume group genus, Qarnet dish genus, wooden lobe genus etc., attract most attention with Annonaceousacetogenicompounds compounds in the contained bioactive ingredients of this section plant.The focus of research is the anticancer and insecticidal activity of the contained Annonaceousacetogenicompounds compounds of this section plant at present.Since nineteen eighty-two first Annona lactone report, found more than 300 of this compounds so far, be considered to the natural compounds of the strong antitumour activity of another class behind the taxol.
Summary of the invention:
The purpose of this invention is to provide plant milk extract of a kind of antidiabetic effect and preparation method thereof.We are index with differentiation myoblastic glucose uptake amount of C2C12 and lactic acid growing amount, extract to one over thousands of kind of natural medicinal plant carries out the anti-diabetic activity screening, found the stronger extract of several activity, wherein multiple is the extract of annonaceae plant, and has determined that by the method that activity is followed the trail of its activeconstituents is an Annonaceousacetogenicompounds compounds.Be expected to be used to be prepared into antidiabetic medicine.The present invention is to be the Annonaceousacetogenicompounds compounds (acetogenins) of raw material extraction with the branches and leaves of annonaceae plant, trunk, bark, root, seed or pericarp.The formulation for preparing antidiabetic medicine with Annonaceousacetogenicompounds compounds (acetogenins) as the anti-diabetic efficient part, can be separately or combine with suitable vehicle etc., make oral dosage form or non-oral dosage form (injection, sprays, patch etc.) according to ordinary method.Preparation method of the present invention is to be raw material with the branches and leaves of annonaceae plant, trunk, bark, root, seed or pericarp, adopt one or more technologies in the technologies such as solvent-extraction process, solvent extration, resin adsorption method, carbon dioxide upercritical fluid extraction, and be prepared into extract in conjunction with conventional drying method such as concentrate drying, drying under reduced pressure or lyophilizes.
In the solvent-extraction process that is adopted, organic solvent or two or more mixed solvents that dissolve each other such as optional water or methyl alcohol, ethanol, propyl alcohol, butanols, amylalcohol, acetone, ethyl acetate, ethyl formate, chloroform, methylene dichloride, ether, at room temperature dipping extracts or with the refluxing extraction under supersound extraction or the heated condition.Extraction time can be once, also can be repeatedly.
In the solvent extration that is adopted, the above-mentioned extract that obtains can be suspended in water directly extracts with suitable solvent (butanols, amylalcohol, ethyl acetate, ethyl formate, chloroform, methylene dichloride, ether etc.).Extraction times can be once, also can be repeatedly.
The resin method that is adopted is the macroporous adsorbent resin method.Water, alcohol or hydrous alcohol extraction thing can be handled with macroporous adsorbent resin, discard the water elution part, collect pure wash-out part promptly.
The carbon dioxide supercritical extraction method that is adopted can according to circumstances add suitable entrainment agent, as, hexanaphthene, chloroform, ethyl acetate, methyl alcohol, ethanol etc.
Judge whether the annonaceae plant milk extract contains Annonaceousacetogenicompounds compounds and can use the thin-layer chromatography development process.Method is: takes from system or commercially available purification on normal-phase silica gel thin layer plate, suitably mixes the solvent that is made into sherwood oil, hexanaphthene, chloroform, ethyl acetate, acetone, methyl alcohol equal solvent and launch (sherwood oil and acetone combination the best), and with following two kinds of chromogenic reagents.Person's prompting contains Annonaceousacetogenicompounds compounds positive findings to the occur simultaneously.
Developer one: Kedde reagent (3, the 5-dinitrobenzoic acid), positive findings disappears very soon for the spot that takes on a red color, color spot.
Developer two: Dragendroff reagent (bismuth potassium iodide), positive findings is for being orange-yellow spot, and the color spot time length is longer.
With the extract made as the anti-diabetic efficient part, can be separately or combine with suitable vehicle etc., make oral dosage form or non-oral dosage form (injection, sprays, patch etc.) is used to prepare the medicine for the treatment of diabetes according to ordinary method.
Advantage of the present invention is: we are index with the myoblastic glucose uptake amount of C2C12 and the milk acid growing amount of differentiation, extract to one over thousands of kind of natural medicinal plant carries out the anti-diabetic activity screening, found the stronger extract of several activity, wherein multiple is the extract of annonaceae plant, and determined that by the method that activity is followed the trail of its activeconstituents is an Annonaceousacetogenicompounds compounds, make it be expected to develop and become a kind of natural antidiabetic medicine, and can be made into multiple formulation.
Description of drawings:
Fig. 1 is promotion activity (n=3) table with test results of corossol extract of the present invention to C2C12 sarcoplast ingestion of glucose and generation lactic acid ability
Embodiment:
Embodiment 1: corossol seed 1kg, with 95% alcohol reflux of 10 times of amounts 2 hours, filter, and concentrate, vacuum dehydrating at lower temperature gets 180g semi-solid extract (yield 18%).This extract has promoter action to the C2C12 cellular uptake glucose of differentiation and the ability of generation lactic acid, and the active testing result as shown in Figure 1.
Claims (7)
1, a kind of plant milk extract of antidiabetic effect is characterized in that: extract is to be the Annonaceousacetogenicompounds compounds (acetogenins) of raw material extraction with the branches and leaves of annonaceae plant, trunk, bark, root, seed or pericarp.
2, a kind of purposes of plant milk extract of a kind of antidiabetic effect as claimed in claim 1, it is characterized in that: the formulation for preparing antidiabetic medicine with Annonaceousacetogenicompounds compounds (acetogenins) as the anti-diabetic efficient part, can be separately or combine with suitable vehicle etc., make oral dosage form or non-oral dosage form according to ordinary method.
3, a kind of preparation method of plant milk extract of a kind of antidiabetic effect as claimed in claim 1, it is characterized in that: it is to be raw material with the branches and leaves of annonaceae plant, trunk, bark, root, seed or pericarp, adopt one or more technologies in the technologies such as solvent-extraction process, solvent extration, resin adsorption method, carbon dioxide upercritical fluid extraction, and be prepared into extract in conjunction with conventional drying method such as concentrate drying, drying under reduced pressure or lyophilizes.
4, the preparation method of the plant milk extract of a kind of antidiabetic effect according to claim 3, it is characterized in that: in the solvent-extraction process that is adopted, organic solvent or two or more mixed solvents that dissolve each other such as optional water or methyl alcohol, ethanol, propyl alcohol, butanols, amylalcohol, acetone, ethyl acetate, ethyl formate, chloroform, methylene dichloride, ether, at room temperature dipping extracts or with the refluxing extraction under supersound extraction or the heated condition, extraction time can be once, also can be repeatedly.
5, the preparation method of the plant milk extract of a kind of antidiabetic effect according to claim 3, it is characterized in that: in the solvent extration that is adopted, the above-mentioned extract that obtains can be suspended in water directly extracts with suitable solvent, and extraction times can be once, also can be repeatedly.
6, the preparation method of the plant milk extract of a kind of antidiabetic effect according to claim 3, it is characterized in that: the resin method that is adopted is the macroporous adsorbent resin method, water, alcohol or hydrous alcohol extraction thing can be handled with macroporous adsorbent resin, discard the water elution part, collect pure wash-out part promptly.
7, the preparation method of the plant milk extract of a kind of antidiabetic effect according to claim 3 is characterized in that: the carbon dioxide supercritical extraction method that is adopted can according to circumstances add suitable entrainment agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031115217A CN1252060C (en) | 2003-04-22 | 2003-04-22 | Sugarapple plant extract with action of resisting diabetes, medicinal application and preparation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB031115217A CN1252060C (en) | 2003-04-22 | 2003-04-22 | Sugarapple plant extract with action of resisting diabetes, medicinal application and preparation method |
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| CN1477102A true CN1477102A (en) | 2004-02-25 |
| CN1252060C CN1252060C (en) | 2006-04-19 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008125928A3 (en) * | 2006-12-21 | 2009-09-11 | Ranbaxy Laboratories Limited | Standardized bioactive extracts of annona squamosa |
| CN101485700B (en) * | 2009-03-03 | 2011-08-31 | 南京中医药大学 | Refined cherimoya total inner ester with anti-tumor activity and preparation method thereof |
| EP2490543A2 (en) * | 2009-10-19 | 2012-08-29 | Laila Nutraceuticals | Extracts, fractions and compositions comprising acetogenins and their applications |
| CN107753532A (en) * | 2017-11-07 | 2018-03-06 | 海南医学院 | A kind of soursop root extract and its anti-diabetes use |
| CN111184032A (en) * | 2020-01-10 | 2020-05-22 | 云南农业大学 | Goniothalamus affinis nematode killing extract as well as preparation method and application thereof |
| CN111317750A (en) * | 2020-02-17 | 2020-06-23 | 江苏卫生健康职业学院 | Application of sweetsop seed total lactone in preparing medicine for preventing and treating diabetes |
| CN111904988A (en) * | 2020-09-23 | 2020-11-10 | 海南医学院 | Application of annona muricata fruit extract in preparation of antidiabetic medicine or health-care product |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070942A (en) * | 2013-02-08 | 2013-05-01 | 中国人民武装警察部队后勤学院 | Application of apple tree branch extract in preparation of hypoglycemic drugs or foods |
-
2003
- 2003-04-22 CN CNB031115217A patent/CN1252060C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008125928A3 (en) * | 2006-12-21 | 2009-09-11 | Ranbaxy Laboratories Limited | Standardized bioactive extracts of annona squamosa |
| CN101485700B (en) * | 2009-03-03 | 2011-08-31 | 南京中医药大学 | Refined cherimoya total inner ester with anti-tumor activity and preparation method thereof |
| EP2490543A2 (en) * | 2009-10-19 | 2012-08-29 | Laila Nutraceuticals | Extracts, fractions and compositions comprising acetogenins and their applications |
| EP2490543A4 (en) * | 2009-10-19 | 2013-02-06 | Laila Nutraceuticals | Extracts, fractions and compositions comprising acetogenins and their applications |
| AU2010309388B2 (en) * | 2009-10-19 | 2016-03-10 | Laila Nutraceuticals | Extracts, fractions and compositions comprising acetogenins and their applications |
| US9427425B2 (en) | 2009-10-19 | 2016-08-30 | Laila Nutraceuticals | Extracts, fractions and compositions comprising acetogenins and their applications |
| CN107753532A (en) * | 2017-11-07 | 2018-03-06 | 海南医学院 | A kind of soursop root extract and its anti-diabetes use |
| CN111184032A (en) * | 2020-01-10 | 2020-05-22 | 云南农业大学 | Goniothalamus affinis nematode killing extract as well as preparation method and application thereof |
| CN111184032B (en) * | 2020-01-10 | 2021-05-07 | 云南农业大学 | Goniothalamus affinis nematode killing extract as well as preparation method and application thereof |
| CN111317750A (en) * | 2020-02-17 | 2020-06-23 | 江苏卫生健康职业学院 | Application of sweetsop seed total lactone in preparing medicine for preventing and treating diabetes |
| CN111904988A (en) * | 2020-09-23 | 2020-11-10 | 海南医学院 | Application of annona muricata fruit extract in preparation of antidiabetic medicine or health-care product |
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| Publication number | Publication date |
|---|---|
| CN1252060C (en) | 2006-04-19 |
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