CN103070942A - Application of apple tree branch extract in preparation of hypoglycemic drugs or foods - Google Patents
Application of apple tree branch extract in preparation of hypoglycemic drugs or foods Download PDFInfo
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- CN103070942A CN103070942A CN2013100506540A CN201310050654A CN103070942A CN 103070942 A CN103070942 A CN 103070942A CN 2013100506540 A CN2013100506540 A CN 2013100506540A CN 201310050654 A CN201310050654 A CN 201310050654A CN 103070942 A CN103070942 A CN 103070942A
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Abstract
The invention discloses application of an apple tree branch extract in the preparation of hypoglycemic drugs or foods. As shown by experimental results, the apple tree branch extract can obviously lower the blood sugar value of a mouse suffering from streptozotocin-induced type 1 diabetes and can obviously increase the tolerance dose of the mouse suffering from the streptozotocin-induced type 1 diabetes to glucose, sucrose and starch.
Description
Technical field
The present invention relates to the new purposes of apple branch extract, particularly relate to the application of apple branch extract in preparation hypoglycemic drug or blood sugar reducing food.
Background technology
The apple branch extract is the extract that the aquiferous ethanol take apple branch as prepared using water or variable concentrations obtains as solvent extraction.The chemical constituent of apple branch extract and pharmacological effect research are less, determine at present to comprise triterpenes and multiple aldehydes matter
[1], wherein polyphenol components is take flavonoid and methods of glycosides thereof as main.The apple branch extract has the effects such as stronger antioxidation, arteriosclerosis
[1]At present, the report of the hypoglycemic activity relevant with Fructus Mali pumilae has the apple polyphenol hypoglycemic activity
[2], the hypoglycemic activity of high-purity phlorizin in the apple branch extract
[3-4], and the hypoglycemic activity of apple branch extract so far there are no the report.
Summary of the invention
The object of the present invention is to provide the application of apple branch extract in preparation hypoglycemic drug or blood sugar reducing food.
Technical scheme of the present invention is summarized as follows:
The application of apple branch extract in preparation hypoglycemic drug or blood sugar reducing food.
Described apple branch extract is to make with following method: be that the ethanol water of 10%-99.9% carries out reflux, extract, with the apple branch of 1 mass fraction with the volumetric concentration of the water of 3-12 mass fraction or 3-12 mass fraction, extract 1-4 time, the each extraction 1-5 hour, filter, filtrate decompression is concentrated, getting pol is the concentrate of 20-40, and drying obtains the apple branch extract.
The kind of described Fructus Mali pumilae be Fuji, state's light, Qiao Najin, Pink Lady, Qin Yang, Qin Guan, loud, high-pitched sound, No. 8, Granny Smith, carbuncle, Red Star, Colophonium any of several broadleaf plants or the U.S..
The dosage form of described hypoglycemic drug is hard capsule, soft capsule, powder, granule, tablet, pill, sweet unguentum, oral liquid, medicated wine, injection or medicinal tea.
Described blood sugar reducing food is beverage, confection, rice food, flour product, meat products, fish product or edible oil.
The results show: the apple branch extract can obviously reduce the blood glucose value of the type 1 diabetes mice that streptozotocin induces.Can obviously improve type 1 diabetes mice that streptozotocin induces to glucose, sucrose and starch dosis tolerata.
Description of drawings
Fig. 1 is that the HPLC of apple branch extract analyzes collection of illustrative plates;
Fig. 2 is that the HPLC of apple polyphenol analyzes collection of illustrative plates.
The specific embodiment
The following examples are in order to make those skilled in the art can better understand the present invention but do not limit the present invention in any way.
The present invention is described further below in conjunction with specific embodiment.
The apple branch method for preparing extractive: be that the ethanol water of 10%-99.9% carries out reflux, extract, with apple branch water or volumetric concentration, filter that filtrate decompression is concentrated, gets concentrate, drying obtains the apple branch extract.With each the different parameter shown in the table 1 apple branch is extracted.
The concrete extracting parameter of apple branch extract among each embodiment of table 1
The liquid phase analysis method of embodiment 15 apple branch extracts (embodiment 1)
The high performance liquid chromatogram condition:
Shimadzu high performance liquid chromatograph (comprise LC-20AT double pump, SPD-M20A UV detector, CTO-20A column oven, Japanese Shimadzu company), phenomenex LunaC18 chromatographic column (250mm * 4.6mm, 5 μ m).
Mobile phase A phase: glacial acetic acid: water=1:99
B phase: acetonitrile: water: glacial acetic acid=70:29.8:0.2,
Gradient elution:
| Time (min) | 0 | 6 | 15 | 30 | 60 | 66 | 80 | 85 | 90 | 100 |
| The |
0% | 5% | 10% | 20% | 26% | 30% | 50% | 60% | 60% | 0% |
Flow velocity: 1.0ml/min;
Detect wavelength: 280nm.
Liquid chromatogram: see Fig. 1
We study and find that different cultivars apple branch extract component composition is similar, and the content of each main component can be slightly variant.The bibliographical information apple polyphenol mainly contains chlorogenic acid, procyanidin class and flavones ingredient
[1]In order to determine the composition difference of apple polyphenol extract and apple branch extract, the liquid chromatogram of we the have utilized high-performance liquid chromatogram determination apple branch extract of same concentrations (weight/volume) (embodiment of the invention 1 preparation) and apple polyphenol (being purchased from Jianfeng Natural Product R﹠D Development Co., Ltd., Tianjin), and its one-tenth is grouped into analyzes test result such as Fig. 1 and 2.Can obviously determine relatively that by Fig. 1, Fig. 2 the apple branch extract is becoming to be grouped into to exist notable difference with apple polyphenol, and have no chlorogenic acid and procyanidin constituents composition in the apple branch extract.Therefore the apple branch extract has hypoglycemic purposes, and it brings into play pharmaceutically-active material base is different from apple polyphenol.
The hypoglycemic activity of the type 1 diabetes mice that embodiment 16 apple branch extracts are induced streptozotocin
Get the type 1 diabetes ICR mice that the streptozotocin about body weight 40g is induced
[5], random packet, 10 every group, be divided into model control group and apple branch extract administration group (being respectively embodiment 1-embodiment 14 preparations), apple branch extract administration group gavage gives apple branch extract 1.5g/kg; Model control group gives isopyknic water.In 0,2h gets blood, measures blood glucose with glucose assays test kit (Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.), calculating mean value, t check analysis result, the administration group relatively has significant difference with blank group.The result is as shown in table 1.Each administration group of apple branch extract can both obviously reduce the blood glucose value of the type 1 diabetes mice that streptozotocin induces at administration 2h.
The hypoglycemic activity (* and model control group ratio, p＜0.001) of the type 1 diabetes mice that table 2 apple branch extract is induced streptozotocin
The impact of the Glucose tolerance test of the type 1 diabetes mice that embodiment 17 apple branch extracts are induced streptozotocin
Get the type 1 diabetes ICR mice that the streptozotocin about body weight 40g is induced
[1], random packet, 10 every group, be divided into model control group and apple branch extract administration group (being respectively embodiment 1-embodiment 14 preparations), apple branch extract administration group gavage gives apple branch extract 1.5g/kg; Model control group gives isopyknic water.Successive administration is after 1 week, animal overnight fasting, 30min after the administration 1 time, then gavage glucose (2g/kg), in administration 0,1,2h gets blood, measures blood glucose with glucose assays test kit (Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.), calculating mean value carries out the t check analysis.The results are shown in Table 2.Experimental result shows the apple branch extract after one week of administration, and the Glucose tolerance test tool of the type 1 diabetes mice that streptozotocin is induced is significantly improved.
The impact (* and model control group ratio, p＜0.005) of the Glucose tolerance test of the type 1 diabetes mice that table 3 apple branch extract is induced streptozotocin
The impact of the sucrose dosis tolerata of the type 1 diabetes mice that embodiment 18 apple branch extracts are induced streptozotocin
Get the type 1 diabetes ICR mice that the streptozotocin about body weight 40g is induced
[1], random packet, 10 every group, be divided into model control group and apple branch extract administration group (being respectively embodiment 1-embodiment 14 preparations), apple branch extract administration group gavage gives apple branch extract 1.5g/kg; Model control group gives isopyknic water.Successive administration is after 1 week, animal overnight fasting, 30min after the administration 1 time, then gavage sucrose (2g/kg), in administration 0,1,2h gets blood, measures blood glucose with glucose assays test kit (Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.), calculating mean value carries out the t check analysis.The results are shown in Table 3.Experimental result shows the apple branch extract after one week of administration, and the sucrose dosis tolerata tool of the type 1 diabetes mice that streptozotocin is induced is significantly improved.
The impact (* and model control group ratio, p＜0.05) of the sucrose dosis tolerata of the type 1 diabetes mice that table 4 apple branch extract is induced streptozotocin
The impact of the starch dosis tolerata of the type 1 diabetes mice that embodiment 19 apple branch extracts are induced streptozotocin
Get the type 1 diabetes ICR mice that the streptozotocin about body weight 40g is induced, random packet, every group 10, be divided into model control group and apple branch extract administration group (being respectively embodiment 1-embodiment 14 preparations), apple branch extract administration group gavage gives apple branch extract 1.5g/kg; Model control group gives isopyknic water.Successive administration is after 1 week, animal overnight fasting, 30min after the administration 1 time, then gavage starch (2g/kg), in administration 0,40min, 130min get blood, measures blood glucose with glucose assays test kit (Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.), calculating mean value carries out the t check analysis.The results are shown in Table 4.Experimental result shows the apple branch extract after one week of administration, and the starch dosis tolerata tool of the type 1 diabetes mice that streptozotocin is induced is significantly improved.
The impact (* and model control group ratio, p＜0.005) of the starch dosis tolerata of the type 1 diabetes mice that table 5 apple branch extract is induced streptozotocin
The effect of lowering blood sugar of embodiment 20 apple branch extracts and phlorhizin relatively
After measured, contain phlorhizin (seeing that the peak that is positioned at the 62min place among Fig. 1 is phlorhizin) in the apple branch extract.Regulate in early days the effect report of blood glucose relevant for phlorhizin.Phlorhizin is natural glucose-sodium cotransporter inhibitor, orally affects its absorption and plays a role.The type 1 diabetes mouse model that we utilize streptozotocin to induce, divide three groups, every group of 10 mices, orally respectively give apple branch extract and normal saline (model control group) that phlorhizin and embodiment 8 obtain and test, phlorhizin gives the amount of contained phlorhizin in the apple branch extract that dosage is equal to embodiment 8 preparations.(having measured the apple branch extract phlorhizin content that embodiment 8 obtains according to the detection method of embodiment 15 is 19.6%.According to the test method of embodiment 16 and the dosage of apple branch extract, the dosage of converting out the phlorhizin sterling is that gavage gives 0.3g/kg phlorhizin (content is 98%), tests according to 16 lower methods of embodiment.Experimental result is as shown in table 6, gives sample after 7 days, and 0 o'clock blood glucose of embodiment 8 administration groups (1.5g/kg) groups has downward trend, again give apple branch extract 2h after, blood glucose descends obviously.The phlorhizin effect then relatively a little less than.The result shows: the effect of apple branch extract oral adjusting blood glucose is not the effect from phlorhizin, but other functional component is playing a role in the apple polyphenol.
Table 6 apple branch extract and phlorhizin oral administration are regulated the effect of blood glucose and are compared
With the apple branch extract of embodiment 1-embodiment 14 preparations, can be prepared into according to a conventional method the hypoglycemic drug of hard capsule, soft capsule, powder, granule, tablet, pill, sweet unguentum, oral liquid, medicated wine, injection or medicinal tea dosage form.
With the apple branch extract of embodiment 1-embodiment 14 preparations, can be prepared into according to a conventional method the blood sugar reducing food of beverage, confection, rice food, flour product, meat products, fish product or edible oil form.
List of references:
[1] Wang Jiao, Song Xinbo, Liu Dailin. the nutritional labeling of Fructus Mali pumilae and health care progress [J]. food research and development, 2011,32(1): 164-168.
[2] Liu Jiechao, Jiao Zhonggao, Wang Sixin. the apple polyphenol extract is to the inhibitory action of α-amylase and alpha-glucosidase. Journal of Fruit Science 2011,28(4): 553 ~ 557.
[3]X.Xia,J.T.Lin,R.K.H.Kinne,Binding?of?phlorizin?to?the?isolated?Cterminal?extramembranous?loop?of?the?Na+/glucose?cotransporter?assessed?by?intrinsic?tryptophan?fluorescence,Biochemistry42(2003)6115–6120.
[4] Dong Huaqiang, Ning Zhengxiang. phlorhizin and diabetes control. food science and technology .2006,12:192-196.
[5]Hidehiko?Beppu,Kan?Shimpo,Takeshi?Chihara,et?al.Antidiabetic?effects?of?dietary?administration?of?Aloe?arborescens?Miller?components?on?multiple?low-dose?streptozotocin-induced?diabetes?in?mice:Investigation?on?hypoglycemic?action?and?systemic?absorption?dynamics?of?aloe?components.Journal?of?Ethnopharmacology103(2006)468-477.
Claims (5)
1. the application of apple branch extract in preparation hypoglycemic drug or blood sugar reducing food.
2. application according to claim 1, it is characterized in that described apple branch extract is to make with following method: be that the ethanol water of 10%-99.9% carries out reflux, extract, with the apple branch of 1 mass fraction with the volumetric concentration of the water of 3-12 mass fraction or 3-12 mass fraction, extract 1-4 time, the each extraction 1-5 hour, filter, filtrate decompression is concentrated, gets the concentrate that pol is 20-40, and drying obtains the apple branch extract.
3. application according to claim 2, the kind that it is characterized in that described Fructus Mali pumilae be Fuji, state's light, Qiao Najin, Pink Lady, Qin Yang, Qin Guan, loud, high-pitched sound, No. 8, Granny Smith, carbuncle, Red Star, Colophonium any of several broadleaf plants or the U.S..
4. application according to claim 1, the dosage form that it is characterized in that described hypoglycemic drug are hard capsule, soft capsule, powder, granule, tablet, pill, sweet unguentum, oral liquid, medicated wine, injection or medicinal tea.
5. application according to claim 1 is characterized in that described blood sugar reducing food is beverage, confection, rice food, flour product, meat products, fish product or edible oil.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115500481A (en) * | 2022-09-29 | 2022-12-23 | 上海纽富莱生物科技有限公司 | Apple branch baked egg product and preparation method thereof |
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| CN1252060C (en) * | 2003-04-22 | 2006-04-19 | 深圳中药及天然药物研究中心 | Sugarapple plant extract with action of resisting diabetes, medicinal application and preparation method |
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| WO2008127646A2 (en) * | 2007-04-11 | 2008-10-23 | Dsm Ip Assets B.V. | Transaminase-based processes for preparation of pregabalin |
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Non-Patent Citations (3)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115500481A (en) * | 2022-09-29 | 2022-12-23 | 上海纽富莱生物科技有限公司 | Apple branch baked egg product and preparation method thereof |
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Application publication date: 20130501 |