CN1475562A - Microalgae breeding equipment - Google Patents

Microalgae breeding equipment Download PDF

Info

Publication number
CN1475562A
CN1475562A CNA031329462A CN03132946A CN1475562A CN 1475562 A CN1475562 A CN 1475562A CN A031329462 A CNA031329462 A CN A031329462A CN 03132946 A CN03132946 A CN 03132946A CN 1475562 A CN1475562 A CN 1475562A
Authority
CN
China
Prior art keywords
water
links
heat exchanger
cultivation tank
tank
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CNA031329462A
Other languages
Chinese (zh)
Other versions
CN1216147C (en
Inventor
孙建明
吴垠
吴斌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN 03132946 priority Critical patent/CN1216147C/en
Publication of CN1475562A publication Critical patent/CN1475562A/en
Application granted granted Critical
Publication of CN1216147C publication Critical patent/CN1216147C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/06Tubular
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes

Abstract

An equipment for culturing microalgae is composed of culturing tank, water supply unit, culture liquid supply unit, air supply unit, CO2 provider, algae liquid collector and artificial light source. Said water supply unit for providing disinfected water consists of water supply pump, water outlet pump, heat exchanger set, water heater, temp-regulating water accumulator, and water source with pump.

Description

A kind of both culturing microalgae equipment
Technical field
The present invention relates to the cultivation equipment of a kind of unicellular little algae.
Background technology
It is 02214168.5 that China Intellectual Property Office discloses a patent No. on March 26th, 2003, and name is called the patent documentation of the culture apparatus of unicellular algae.This device is by cultivation tank, and the nutrient solution supply that has that links with it divides device, and the water supply divides device, and air and carbonic acid gas supply divide device, and the collection of algae liquid divides device and source of artificial light to divide device to form.This technology has following shortcoming, owing to be continuous cultivating microalgae, needs a large amount of water through sterilization constantly to inject cultivation tank, and the prior art is used ultraviolet sterilizer.This Sterilizers is not high to microorganism and the protozoon killing rate that influences micro-algae propagation and growth, and mentioned microorganism that it is not killed and protozoon will breed rapidly in cultivation tank and grow.Therefore, through must clearing up completely cultivation tank after a while, along with the increase of clearing times will influence output and increase production cost.If with the chemical process sterilization, then in the sterilization tank of special use, add clorox, dioxide peroxide, added hypo again through 12~24 hours and remove chlorine residue; Perhaps adding hydrochloric acid and acid adjustment basicity in the water of sterilization tank is below the PH2, and making potential of hydrogen through repeated hydrogenation sodium oxide after 24 hours is to finish sterilizing works about PH8.This sterilising method length that takes time, it is not thorough to sterilize, and at least two complete equipments will be arranged, and in water residual chemicals also, unfavorable to both culturing microalgae; In addition, if breeding of little algae and growth in optimum temperature range, its breeding and growth rate also the maximum, but prior art is not taken measures to above-mentioned optimum temperature range, therefore, in winter during cultivating microalgae, its seawater water temperature is the end very, water cultivating microalgae output with this temperature is very low, in summer during cultivating microalgae, the water temperature of seawater is higher, and cultivation tank is subjected to the irradiation of high light in summer, its temperature can be up to 40 ℃, even need the very high salt algae of temperature also can normally numerous and growth.
Summary of the invention
The object of the invention is to provide a kind of both culturing microalgae equipment for the shortcoming that overcomes prior art, and the breeding water that is provided in its continuity breeding process can reach almost hundred-percent other microorganisms that influence both culturing microalgae of killing; The present invention can also be provided with heat radiation/heat-absorbing device in cultivation tank, its hot/cold source is arranged on outside the cultivation tank, makes algae liquid in the both culturing microalgae equipment be in the best breeding temperature range of little algae, the little algae of production that can continuous high-efficient.
The present invention can reach by following measure: a kind of both culturing microalgae equipment, comprise cultivation tank, and by the water supply that pipeline links with it, nutrient solution feeding mechanism, air supply device, carbonic acid gas feeding mechanism, algae liquor collecting device and source of artificial light; Be characterized in that described water supply is to be connected by following device to form; The water-in and the water source of working shaft link, the cooling water inlet of its water outlet and heat exchanger set links, the cooling water inlet of water heater and the cooling water outlet of heat exchanger set link, the hot water inlet of its hot water outlet and heat exchanger set links, going out the water-in of water pump and the hot water outlet of heat exchanger set links, its outlet links with the hot water inlet of temperature adjustment water tank, and a water-in of temperature adjustment water tank hot water outlet and cultivation tank links, and temperature adjustment water tank mouth cold water cycles through pump and the water source links.The present invention also has a register, and its heat radiation/heat-absorbing device is arranged in the algae liquid of cultivation tank, and its hot/cold source is positioned at outside the cultivation tank.
The present invention compared with prior art has following advantage: from described solution as can be seen, because water heater can be heated to water 85~100 ℃ of high temperature continuously, be enough to kill microorganisms all in the water and other protozoons, be heated disinfectant water and pass through heat exchanger set again continuously, at last the temperature that enters cultivation tank by the temperature adjustment water tank is adjusted into the optimum growth temp of cultivating microalgae, check through routine, the handled water of water supply of the present invention, its protozoon killing rate is 99.99~100%, and bacterial inactivation rate is 99.99~100%.Water in the cultivation tank contains protozoon and amount of bacteria is almost nil owing to enter.Therefore, can be extended down to more than half a year the pitch time of cultivation tank cleaning.When passing through heat exchanger set owing to the water that comes out from water heater again, its heat is absorbed by cold water to be heated, if heat exchanger set is three grades, the water heater Heating temperature reaches 90 ℃, then the cooling water inlet temperature of heat exchanger set is 20 ℃, and 30 ℃ of hot water outlet temperature, hot water inlet temperature are 90 ℃, cold water outlet temperature is 70 ℃, so water supply installation of the present invention can also be saved a large amount of heat energy.It can also be seen that from solution of the present invention, because heat radiation/heat-absorbing device is housed in cultivation tank, its hot/cold source is positioned at outside the cultivation tank, this improved result can make the algae liquid in the cultivation tank be in the optimum temperature range of micro-algae propagation and growth always, and the output of its little algae can obtain large increase.
Description of drawings
Fig. 1 is the structural arrangement skeleton diagram of a kind of both culturing microalgae equipment of the present invention;
Fig. 2 is a water supply structural representation of the present invention.
Embodiment
Now in conjunction with the embodiments (accompanying drawing) the invention will be further described: as shown in Figure 1, 2,1 sealing cultivation tank for the polygon prism tube made by glass, production well 16 is arranged at its top, water-in 18 and nutrient solution import 17, algae liquid outlet 19 is arranged in lateral middle and lower part, near its bottom carbon dioxide gas import 23 is arranged, the gas lift stirring pipe 34 that links with air intlet 22 that air intlet 22 is arranged and be positioned at cultivation tank near lateral middle part; 4 is air supply device, is by pneumatic pump 28, air filter 27, and flow valve 26 and air-lift tube 34 are formed; 5 is the carbonic acid gas feeding mechanism, and by carbon dioxide steel cylinder 24, flow valve 25 is formed; 6 is the algae liquor collecting device, is made up of the holding tank 21 and the flow valve 20 of algae liquid; 7 is source of artificial light, and it is a kind of submarine light of waterproof, selects the light fixture of different light waves according to little algae of being cultured; 8 is register, and heat radiation/heat-absorbing device 32 is loaded on the middle and lower part of cultivation tank 1, and its hot/cold source 33 is positioned at outside the cultivation tank 1; 3 is the nutrient solution feeding mechanism, and its wort cylinder 14 is connected in series with flow valve 31 and links by nutrient solution import 17 and cultivation tank; 2 is water supply, formed by following device connection: the water-in of working shaft 9 and water source 29 link, the cooling water inlet of its water outlet and heat exchanger set 10 links, water heater 11 is an electric heater, the cooling water outlet of its cooling water inlet and heat exchanger set 10 links, the hot water inlet of its hot water outlet and heat exchanger set 10 links, going out the water-in of water pump 12 and the hot water outlet of heat exchanger set 10 links, its outlet links with the hot water inlet of temperature adjustment water tank 13, a water-in 18 of the hot water outlet of temperature adjustment water tank 13 and cultivation tank 1 links, and its cold water circulation is to link by pump 30 and water source 29.Heat exchanger set of the present invention is connected by three-stage heat exchanger, and the progression of heat exchanger set is many more, and its energy-saving effect is good more; Water supply of the present invention, the hot/cold source of register, nutrient solution feeding mechanism, air supply device, carbonic acid gas feeding mechanism, algae liquor collecting device and light source can be distinguished a plurality of cultivation tank in parallel simultaneously, to reach the batch process of scale.The present invention is work like this: starting water supply 2, promptly open working shaft 9 and electric heater 11 and heat water to 90 ℃, when treating to have in the electric heater a certain amount of hot water, open out water pump 12 and pump 30, hot water in the electric heater is cooled to 30 ℃ and enter in the temperature adjustment water tank 13 through three placed in-line heat exchanger set 10, enter in the cultivation tank 1 through water-in 18 through further temperature adjustment to the only temperature of micro algae growth, when the water that injects cultivation tank reached a certain amount of, water monitor cut out water-in 18; The nutrient solution that deposits in advance in the wort cylinder 14 is sent into nutrient solution by flow valve 31 and water-in 17 to cultivation tank 1, inject little algae seed by production well 16 to cultivation tank, make its initial density for when results density 1/4, open source of artificial light 7, register 8, make its algae liquid temp reach just when, carbonic acid gas feeding mechanism 5 and air supply device 4, since air air-lift tube 34 in on mobile, algae liquid below the drive air-lift tube upwards flows along air-lift tube, thereby the algae liquid in the cultivation tank is produced stirring action, after 7 days, the density of algae liquid reaches results density, and opening flow valve 20 and adjusting its flow is to flow out every day to be fully loaded with 1/8 of algae liquid in the cultivation tank 1.

Claims (2)

1, a kind of both culturing microalgae equipment, comprise cultivation tank (1), the water supply (2) that links with it by pipeline, nutrient solution feeding mechanism (3), air supply device (4), carbonic acid gas feeding mechanism (5), algae liquor collecting device (6) and source of artificial light (7), it is characterized in that: described water supply (2) is to be connected by following device to form: the water-in of working shaft (9) and water source (29) link, the cooling water inlet of its water outlet and heat exchanger set (10) links, the cooling water outlet of the cooling water inlet of water heater (11) and heat exchanger set (10) links, and the hot water inlet of its hot water outlet and heat exchanger set (10) links.Going out the water-in of water pump (12) and the hot water outlet of heat exchanger set (10) links, its outlet links with the hot water inlet of temperature adjustment water tank (13), a water-in (18) of the hot water outlet of temperature adjustment water tank (13) and cultivation tank (1) links, and its cold water circulation is to finish by pump (30).
2, a kind of both culturing microalgae equipment according to claim 1 is characterized in that: also have a register (8), its heat radiation/heat-absorbing device (32) is arranged in the algae liquid of cultivation tank (1), and its hot/cold source (33) is positioned at outside the cultivation tank (1).
CN 03132946 2003-07-24 2003-07-24 Microalgae breeding equipment Expired - Fee Related CN1216147C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 03132946 CN1216147C (en) 2003-07-24 2003-07-24 Microalgae breeding equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 03132946 CN1216147C (en) 2003-07-24 2003-07-24 Microalgae breeding equipment

Publications (2)

Publication Number Publication Date
CN1475562A true CN1475562A (en) 2004-02-18
CN1216147C CN1216147C (en) 2005-08-24

Family

ID=34154203

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 03132946 Expired - Fee Related CN1216147C (en) 2003-07-24 2003-07-24 Microalgae breeding equipment

Country Status (1)

Country Link
CN (1) CN1216147C (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100419066C (en) * 2006-04-14 2008-09-17 中国科学院武汉植物园 Arrangement for supplementing CO2 to micro-algae culture pond
CN100435623C (en) * 2006-06-14 2008-11-26 张全铭 A method for breeding spirulina
CN102268375A (en) * 2011-06-03 2011-12-07 中国海洋大学 Method and system for continuously collecting algae liquid cultured from unicellular algae
CN102286362A (en) * 2011-07-11 2011-12-21 鞠志国 Fully sealed floor heating type runway pool microalgae culture system
CN102674486A (en) * 2012-05-29 2012-09-19 山东安源水产股份有限公司 Heating disinfection method and device for microalgae cultivating water
CN102758027A (en) * 2011-04-28 2012-10-31 杭州汉徽光电科技有限公司 Generation environment control method and system for microalgae cultivation
CN103112993A (en) * 2013-01-31 2013-05-22 胜利油田胜利勘察设计研究院有限公司 Method for processing oilfield wastewater and fixing CO2 (carbon dioxide) by using microalgae
CN103626362A (en) * 2013-12-05 2014-03-12 南通大学 System for treating urban waste water in recycling manner
CN105143431A (en) * 2013-03-13 2015-12-09 罗盖特公司 Bioreactor
CN106754289A (en) * 2015-11-19 2017-05-31 国家开发投资公司 Dewatering microalgae device and method based on positive penetration theory

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI381046B (en) * 2009-09-28 2013-01-01 Univ Ishou Airlift type light reaction device
US8222025B2 (en) 2010-03-23 2012-07-17 Lan Wong Multistory bioreaction system for enhancing photosynthesis

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100419066C (en) * 2006-04-14 2008-09-17 中国科学院武汉植物园 Arrangement for supplementing CO2 to micro-algae culture pond
CN100435623C (en) * 2006-06-14 2008-11-26 张全铭 A method for breeding spirulina
CN102758027A (en) * 2011-04-28 2012-10-31 杭州汉徽光电科技有限公司 Generation environment control method and system for microalgae cultivation
CN102268375B (en) * 2011-06-03 2012-10-03 中国海洋大学 Method and system for continuously collecting algae liquid cultured from unicellular algae
CN102268375A (en) * 2011-06-03 2011-12-07 中国海洋大学 Method and system for continuously collecting algae liquid cultured from unicellular algae
CN102286362A (en) * 2011-07-11 2011-12-21 鞠志国 Fully sealed floor heating type runway pool microalgae culture system
CN102674486A (en) * 2012-05-29 2012-09-19 山东安源水产股份有限公司 Heating disinfection method and device for microalgae cultivating water
CN103112993A (en) * 2013-01-31 2013-05-22 胜利油田胜利勘察设计研究院有限公司 Method for processing oilfield wastewater and fixing CO2 (carbon dioxide) by using microalgae
CN105143431A (en) * 2013-03-13 2015-12-09 罗盖特公司 Bioreactor
CN105143431B (en) * 2013-03-13 2018-01-23 罗盖特公司 bioreactor
US10400207B2 (en) 2013-03-13 2019-09-03 Roquette Freres Bioreactor
CN103626362A (en) * 2013-12-05 2014-03-12 南通大学 System for treating urban waste water in recycling manner
CN103626362B (en) * 2013-12-05 2016-01-13 南通大学 A kind of system of recycling treatment municipal wastewater
CN106754289A (en) * 2015-11-19 2017-05-31 国家开发投资公司 Dewatering microalgae device and method based on positive penetration theory

Also Published As

Publication number Publication date
CN1216147C (en) 2005-08-24

Similar Documents

Publication Publication Date Title
CN1216147C (en) Microalgae breeding equipment
KR101423285B1 (en) system and method for cultivating micro algae using wastewater
KR100866985B1 (en) Device and method for cultivating microorganism
KR101136015B1 (en) Remote Control System of Microbial incubator
CN105859051B (en) A kind of biogas slurry light processing cultivating system and its operational method
AU2010313192A1 (en) Photobioreactor system and method of using the same
CN100402122C (en) Sea filter device with mighty ultraviolet disinfection
CN102668952A (en) Sterilization method of soilless culture medium
KR20100113179A (en) Tubular-type apparatus for cultivating spirulina sp
JP5657938B2 (en) Circulating culture method for photosynthetic microalgae
CN104789478B (en) A kind of cultural method and culture systems of haematococcus pluvialis algae kind
CN205368387U (en) Medical science detects uses microbiological incubator
CN106993574A (en) A kind of sea base combined-circulation fish dish edible mushroom cogeneration system
JP5324532B2 (en) Circulating photobioreactor
CN218951379U (en) Indoor proliferation culture system of haematococcus pluvialis
CN103548577B (en) System and method for producing and culturing liquid spawns of edible fungus
JP5981653B2 (en) Bioreactor vessel for large-scale growth of plants under aseptic conditions
JP2019076004A (en) Algae culture method and algae culture plant
CN210494657U (en) Tide type water and fertilizer disinfection and sterilization device
CN201150216Y (en) Marine product living body cultivation exhibiting device
CN211960599U (en) Shrimp pond recirculating aquaculture system
CN2578308Y (en) Water heater sterilizer for microalgae culture
CN204727882U (en) A kind of Haematocoocus Pluvialls algal species cultivation system
CN114145260A (en) Ecological breeding circulating system
CN105284486B (en) A kind of artificial climate growth chamber that plant products can be improved

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract

Assignee: Dalian Huixin Titanium Equipment Development Co., Ltd.

Assignor: Sun Jianming|Wu Ken|Wu Bin|Sang Yaoqing

Contract fulfillment period: 2008.10.15 to 2013.10.14

Contract record no.: 2008990001054

Denomination of invention: Microalgae breeding equipment

Granted publication date: 20050824

License type: Exclusive license

Record date: 2008.11.3

LIC Patent licence contract for exploitation submitted for record

Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.10.15 TO 2013.10.14; CHANGE OF CONTRACT

Name of requester: DALIAN HUIXIN TITANIUM EQUIPMENT DEVELOPMENT CO.,

Effective date: 20081103

EM01 Change of recordation of patent licensing contract

Change date: 20131105

Contract record no.: 2008990001054

Assignor after: Sun Jianming

Assignor after: Wu Yin

Assignor after: Wu Bin

Assignor before: Sun Jianming

Assignor before: Wu Yin

Assignor before: Wu Bin

Assignor before: Sang Yaoqing

LICC Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model
EM01 Change of recordation of patent licensing contract

Change date: 20131105

Contract record no.: 2008990001054

Assignor after: Sun Jianming

Assignor after: Wu Yin

Assignor after: Wu Bin

Assignor before: Sun Jianming

Assignor before: Wu Yin

Assignor before: Wu Bin

Assignor before: Sang Yaoqing

LICC Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20050824

Termination date: 20180724

CF01 Termination of patent right due to non-payment of annual fee