CN1471966A - Glossy ganoderma spore powder preparing method - Google Patents
Glossy ganoderma spore powder preparing method Download PDFInfo
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- CN1471966A CN1471966A CNA031396739A CN03139673A CN1471966A CN 1471966 A CN1471966 A CN 1471966A CN A031396739 A CNA031396739 A CN A031396739A CN 03139673 A CN03139673 A CN 03139673A CN 1471966 A CN1471966 A CN 1471966A
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- ganoderma
- ganoderma spore
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- cellular wall
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Abstract
A process for preparing the ganoderma spore powder includes such steps as choosing ganoderma seeds, culturating in wild environment, carefully choosing ganoderma spora, immersing them in culture medium until they are germinated, and low-temp. physically breaking their walls. Its advantage is high quality of product.
Description
Technical field
The present invention relates to the science process technology of Chinese medicine natural drug, refer in particular to a kind of natural glossy ganoderma according to its biological characteristics, from the screening and the cultivation of strain, cultivation is to selected spore, optimize and sprout, a whole set of scientific method of low temperature breaking cellular wall is guaranteed the method for high-quality Ganoderma spore powder preparation.
Background technology
Ganoderma is a Basidiomycetes, Polyporaceae Ganoderma fungus.Ganoderma is made up of mycelium and sporophore.Hyphae colorless is transparent, has branch.Sporophore is constantly grown by mycelium and kink forms.It comprises stem, cap and three parts of hymenium.After the Ganoderma maturation, the Sporulation of Ganoderma is in the hymenium at the cap back side, and spore is brown, and it is avette, spherical or subsphaeroidal that profile is.After Ganoderma spore release (launching) is come out, under adapt circumstance and edaphic condition, finish the sexual propagation of Ganoderma.Ganoderma is precious medicinal fungus, and medicine and pharmacology of all times think that all Ganoderma has the body resistance strengthening and constitution consolidating, heart tonifying key brain, protect the liver, functions such as calmness, to coronary heart disease, diseases such as chronic tracheitis, angina pectoris, hypertension, neurasthenia and hepatitis all have curative effect in various degree.Put down in writing in the Compendium of Material Medica: " the Ganoderma bitter in the mouth, flat, nontoxic, the beneficial motive is invigorated blood circulation, and goes into the heart, and hyperemia helps the heart to fill arteries and veins, calms the nerves, lung benefiting gas, invigorating middle warmer increases wisdom, good color, sharp joint, bone and muscle strengthening is eliminated the phlegm, the key stomach ".Particularly the Ganoderma spore powder with cellular wall broken of medical circle utilization in recent years is remarkable as the Chinese medicine effect of treatment cancer, short several years, approved by numerous tumor patients, and also enjoyed higher reputation in the world, generally believed that the wall-breaking lucidum powder is the active drug of anti-curing oncoma.
Summary of the invention
The object of the present invention is to provide a kind of method for preparing the high-quality Ganoderma spore powder.
Guiding theory of the present invention has been based on good kind, and the raw material that just has has had good raw material, follow-up processing (as the spore flotation, cultivate and sprout, the low temperature breaking cellular wall) is just meaningful, the GMP management of each link of technological process has been arranged, could prepare high-quality natural glossy ganoderma spore powder.
The object of the present invention is achieved like this: a kind of method for preparing Ganoderma spore powder may further comprise the steps successively:
1) select the female kind of Ganoderma, and by imitative wild environment cultivation;
2) selected Ganoderma spore;
3) selected Ganoderma spore is soaked sprouting in culture medium;
4) Ganoderma spore is carried out low-temperature physics method breaking cellular wall, make Ganoderma spore powder.
Screening varieties in the step 1) of the present invention, the Ganoderma of selecting for use are meant the spore that launch with the Korea S Ganoderma lucidum (Leyss. Ex Fr.) Karst. G10 section Ben Lingzhi ripening period of the imitative wild cultivation of artificial vaccination in high mountain Ganoderma exploitation base.The Ganoderma spore of G10 kind, it is characterized in that full seed, appearance is cunning, special bitterness and faint scent is arranged, according to check and analysis, it is more that it contains the active substance of unsaturated bond, in the lipid of its extraction, oleic acid and linoleic content account for more than 85% of total amount, also contain sterol, enol, various active compositions such as triterpenes.The growing environment of Ganoderma is about about the 1000 meters mountain area of height above sea level, and this ecological environment and Ganoderma biological property adapt.
The present invention consider Ganoderma spore gather and drying process in practical situation, contain some impurity in the raw material Ganoderma spore, comprise light impurity dust etc., the heavy seeds grains of sand or clod etc., this one procedure of selected spore of still needing.According to the different specific weight of impurity and Ganoderma spore, can adopt the gravity type refiner, centrifugal refiner, wind-force refiner or vibration refiner are handled.To adopt the aqueous medium flotation device is example, at first washing screening, follow classified filtering, gradient is by 200～500 eye mesh screens, remove large and small impurity, dry and centrifuge drying, centrifuge speed 4500r/min, at last temperature below 40 ℃ baking oven or drying room in dry, control Ganoderma spore moisture is about 5%.
Place culture fluid to soak clean, the full Ganoderma spore after selected, soak time 1～8 hour.The imbibition of spore elder generation grows germ tube under the moistening of culture fluid, this calls spore germination.The process conditions that Ganoderma spore is sprouted in sprouting the chamber are as follows: (1) sprouts the chamber through disinfection by ultraviolet light, and air needs just can enter sprouting chamber, well-ventilated through purified treatment.(2) the constant temperature and humidity device of control can accurately keep sprouting the temperature required and humidity in chamber automatically, and the temperature that Ganoderma spore is sprouted is controlled at 20～40 ℃, relative humidity 70～95%.(3) the work bedstead of standard is arranged, each bedstead is established 3～10 layers, puts pallet for spore germination for every layer, and every layer at a distance of 0.2～0.5 meter, bedstead is accomplished firm, smooth, safety.(4) culture medium has following three kinds, is respectively culture medium A: malt extract 20g, and mycelium extracting solution 200ml, defatted wheat germ juice 300ml, natural coconut palm juice 300ml adds water to 1000ml to dissolving; Culture medium B: peeled potatoes powder 100g, agar 15g, sucrose 20g adds water to 1000ml, is heated with stirring to dissolving; Culture medium C: starch 50g, glucose 10g, malt extract 20g, agar 15g adds water to 1000ml, is heated with stirring to dissolving.(5) sprout time is 1～72 hour, and germination rate adopts the S-510 of Hitachi type ultramicroscope to detect, or replaces ultramicroscope to detect with the micro-amplification analytical system of computer.
Adopt physical method to carry out broken wall treatment the Ganoderma spore of sprouting, the wall-breaking method involving vibrations galls wall, and the ring roller grinds breaking cellular wall, jet mill breaking cellular wall etc.; With the dither mill is example, adopts special titanium alloy rod medium, filling rate 60～90%, and vibromill adds cooling system, makes the breaking trachytectum of glossy ganoderma process control at the warm area below 30～-10 ℃.Acceleration when medium impacts material is 10～20g, and the process time is 1～60 minute; Finished product through the processing of dither mill is emitted through discharge nozzle, and particle diameter can reach below 1 micron, and its breaking cellular wall situation can detect with 10,000 times of ultramicroscope photographies, and sporoderm-broken rate reaches more than 99.0%.Ganoderma spore powder through breaking cellular wall passes through a magnetic force machine, under multilamellar magnetic bar suction, removes residual metal impurity, is delivered to packing shop at last and packs, and can make hard capsule or other dosage form.Handle through terminal sterilization before the product export; According to Ministry of Public Health issue "
60Co radiation sterilization standard " carry out dosage 2～6KGY radiation sterilization at radiation chamber, exposure time 0.5～8 hour.
The present invention compared with prior art; characteristics are optimizations of each link of whole process of production; for preparing high-quality Ganoderma spore powder; screening varieties is listed in first procedure of this method; to obtain fine spore simultaneously, cultivate, and not be damaged for the physiologically active ingredient of guaranteeing Ganoderma spore powder and adopt the physical method breaking cellular wall by pseudo-wild cultivating method; and under cooling system protection, carrying out effective breaking cellular wall in 30～-10 ℃, its sporoderm-broken rate reaches more than 99.0%.
Description of drawings
Fig. 1 is a process flow diagram of the present invention.
The specific embodiment
Below in conjunction with drawings and Examples the present invention is done to describe further.
As shown in Figure 1, a kind of method for preparing Ganoderma spore powder of the present invention may further comprise the steps successively:
1) preferred Ganoderma is female plants, and by imitative wild environment cultivation;
2) selected Ganoderma spore;
3) selected Ganoderma spore is soaked sprouting in culture medium;
4) Ganoderma spore is carried out low-temperature physics method breaking cellular wall, make Ganoderma spore powder.
Embodiment 1:
1) G10 number female kind of introducing a fine variety of planting of screening imitated the high-quality spore 10Kg that wild high mountain Ganoderma is produced.
2) through flotation, stepwise gradient filters, and reaches further optimization purpose.
3) will put into culture medium A through selected Ganoderma spore soaks, at room temperature soak to be placed in 2 hours and sprout in the chamber, sprout room temperature and be controlled at 26 ℃, relative humidity is controlled at 85%, (it was good to note sprouting chamber ventilated in 32 hours for sprout time, air is sent into after purified treatment and is sprouted the chamber), detect through Electronic Speculum, germination rate reaches 95%.
4) through dither mill micronizing, cooling system recirculated water (containing 15% second=alcohol) is controlled at-5 ℃ of filling rates 80%, and detecting sporoderm-broken rate by 10,000 times of Electronic Speculum is more than 99.0%.
5) regain Ganoderma spore powder with cellular wall broken 8.5Kg.
Embodiment 2:
Similar to embodiment 1, different is to put into culture medium B through selected Ganoderma spore to soak, and sprout time is extended for 36 hours, and the germination rate of this moment can reach 95%.Through dither mill micronizing, detect sporoderm-broken rate by 10,000 times of Electronic Speculum and reach more than 99.0% equally.
Embodiment 3:
Similar to embodiment 1, different is to put into culture medium C through selected Ganoderma spore to soak, and sprout time is 35 hours, and the germination rate of this moment can reach 95%.Through dither mill micronizing, detect sporoderm-broken rate by 10,000 times of Electronic Speculum and reach more than 99.0% equally.
Embodiment 4:
Similar to embodiment 1, different is that at room temperature soak time is 3 hours, sprouts room temperature and is controlled at 30 ℃, and relative humidity is controlled at 90%, and sprout time is 30 hours, and germination rate can reach 95%.Through dither mill micronizing, detect sporoderm-broken rate by 10,000 times of Electronic Speculum and reach more than 99.0% equally.
Embodiment 5:
Similar to embodiment 2, different is that at room temperature soak time is 3 hours, sprouts room temperature and is controlled at 30 ℃, and relative humidity is controlled at 90%, and sprout time is 30 hours, and germination rate can reach 95%.Through dither mill micronizing, detect sporoderm-broken rate by 10,000 times of Electronic Speculum and reach more than 99.0% equally.
Embodiment 6:
Similar to embodiment 5, different is to put into culture medium C through selected Ganoderma spore to soak, and germination rate can reach 95%.Through dither mill micronizing, detect sporoderm-broken rate by 10,000 times of Electronic Speculum and reach more than 99.0% equally.
Claims (4)
1. method for preparing Ganoderma spore powder may further comprise the steps successively:
1) preferred Ganoderma is female plants, and by imitative wild environment cultivation;
2) selected Ganoderma spore;
3) selected Ganoderma spore is soaked sprouting in culture medium;
4) Ganoderma spore is carried out low-temperature physics method breaking cellular wall, make Ganoderma spore powder.
2. method according to claim 1 is characterized in that: the preferred lucidum variety of step 1) is Korea S Ganoderma lucidum (Leyss. Ex Fr.) Karst. G10 number female the kind, by imitative wild, meets the ecological environment cultivation of 1000 meters left and right sides of height above sea level.
3. method according to claim 1 is characterized in that: the immersion of step 3) is sprouted process conditions and is: soaking at room temperature, soak time 1～8 hour; Sprout time 1～72 hour, 20～40 ℃ of temperature, relative humidity 70～95%; Culture medium has three kinds, is respectively culture medium A: malt extract 20g, and mycelium extracting solution 200ml, defatted wheat germ juice 300ml, natural coconut palm juice 300ml adds water to 1000ml to dissolving; Culture medium B: peeled potatoes powder 100g, agar 15g, sucrose 20g adds water to 1000ml, is heated with stirring to dissolving; Culture medium C: starch 50g, glucose 10g, malt extract 20g, agar 15g adds water to 1000ml, is heated with stirring to dissolving.
4. method according to claim 1 is characterized in that: the described low-temperature physics breaking cellular wall of step 4) involving vibrations galls wall, and the ring roller grinds breaking cellular wall, jet mill breaking cellular wall etc., and temperature is between 30～-10 ℃.
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| CNA031396739A CN1471966A (en) | 2003-07-02 | 2003-07-02 | Glossy ganoderma spore powder preparing method |
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| CNA031396739A CN1471966A (en) | 2003-07-02 | 2003-07-02 | Glossy ganoderma spore powder preparing method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766593A (en) * | 2012-07-25 | 2012-11-07 | 福建省健神生物工程有限公司 | Ganoderma spore purification method |
| CN102793728A (en) * | 2012-08-24 | 2012-11-28 | 安徽哈博药业有限公司 | High-frequency oscillation wall-broken ganoderma lucidum spore powder |
| CN104820073A (en) * | 2015-05-05 | 2015-08-05 | 李朝谦 | Method for quickly and simply detecting impurities of ganoderma lucidum spore powder |
| CN105018532A (en) * | 2014-04-28 | 2015-11-04 | 大自然生机股份有限公司 | Ganoderma lucidum liquid state culture medium and application thereof |
| CN106635804A (en) * | 2016-12-07 | 2017-05-10 | 江苏天丰生物科技有限公司 | Wall breaking method of ganoderma lucidum spore powder |
| CN110438008A (en) * | 2019-09-04 | 2019-11-12 | 钟志强 | A kind of ganoderma lucidium spore powder wall breaking method of granulating |
-
2003
- 2003-07-02 CN CNA031396739A patent/CN1471966A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766593A (en) * | 2012-07-25 | 2012-11-07 | 福建省健神生物工程有限公司 | Ganoderma spore purification method |
| CN102793728A (en) * | 2012-08-24 | 2012-11-28 | 安徽哈博药业有限公司 | High-frequency oscillation wall-broken ganoderma lucidum spore powder |
| CN105018532A (en) * | 2014-04-28 | 2015-11-04 | 大自然生机股份有限公司 | Ganoderma lucidum liquid state culture medium and application thereof |
| CN104820073A (en) * | 2015-05-05 | 2015-08-05 | 李朝谦 | Method for quickly and simply detecting impurities of ganoderma lucidum spore powder |
| CN106635804A (en) * | 2016-12-07 | 2017-05-10 | 江苏天丰生物科技有限公司 | Wall breaking method of ganoderma lucidum spore powder |
| CN110438008A (en) * | 2019-09-04 | 2019-11-12 | 钟志强 | A kind of ganoderma lucidium spore powder wall breaking method of granulating |
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Owner name: ZHONG LIANGYU Free format text: FORMER OWNER: BAOXING BIOLOGICAL SCIENCE + TECHNOLOGY CO., LTD., GUANGZHOU CITY Effective date: 20040709 |
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Effective date of registration: 20040709 Address after: Tianzi Plaza No. 203 River Road Guangzhou city Guangdong province 510115 room 1510 Applicant after: Zhong Liangyu Address before: 510600, room 12, building 108, Feng Wei building, right new street, No. 5 new temple road, Wuyang Town, Guangdong,, Guangzhou Applicant before: Baoxing Biological Science & Technology Co., Ltd., Guangzhou City |
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