CN1463987A - Process for extracting aloinose - Google Patents
Process for extracting aloinose Download PDFInfo
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- CN1463987A CN1463987A CN 02114216 CN02114216A CN1463987A CN 1463987 A CN1463987 A CN 1463987A CN 02114216 CN02114216 CN 02114216 CN 02114216 A CN02114216 A CN 02114216A CN 1463987 A CN1463987 A CN 1463987A
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- aloe
- juice
- filtering
- extracting
- collect
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The aloe polysaccharide extracting process includes pulping aloe leaf into juice, filtering, centrifuging, circulating concentration to obtain concentrated aloe gel, mixing aloe gel with alcohol via stirring, letting stand for 1-3 hr, filtering to collect coarse aloe polysaccharide and recover alcohol, washing filtering cloth to obtain washing liquid, merging washing liquid and coarse aloe polysaccharide, dissolving via stirring to obtain water solution of coarse aloe polysaccharide, filtering to obtain filtrate liquid, ultrafiltering and nano filtering with ultrafiltering film and nano filtering film, collecting concentrated liquid as the product. The said process can separate aloe polysaccharide as most while maintaining its activity, and may be used in mass production.
Description
Technical field
The present invention relates to from Fresh leaf of aloe or Aloe gel inspissated juice, extract the preparation technology of Aloe polysaccharide.
Background technology
Aloe extract is the important source material of medicine, chemical industry, food aspect, and Aloe polysaccharide is one of main effective component during aloe is extracted.But Aloe polysaccharide content is very low, and difficulty is very big when separation, extraction, purifying.In the laboratory, traditional method is to extract aloe coarse polysaccharid with ethanol precipitation, separate by glucose gel then, but this method fails to be put to produce.The separation, extraction, the purification technique that improve Aloe polysaccharide are the prerequisites of batch preparations high quality Aloe polysaccharide.
Summary of the invention
The technical problem that will solve required for the present invention is will provide a kind of can isolate Aloe polysaccharide to greatest extent from aloe, not only can save energy but also can keep its original activity to extract purifying, produces the technology of extracting Aloe polysaccharide in batches.
Technical scheme of the present invention is: with the Fresh leaf of aloe of treated mistake smash starch into Folium Aloe juice after-filtration, centrifugal, circulate after concentrating the Aloe gel inspissated juice, in the Aloe gel inspissated juice, add 9 times of amount 95% ethanol, normal temperature fully stirs down, left standstill 1-3 hour, filter, reclaim ethanol, collect aloe coarse polysaccharid, filter cloth washes with water 3 times, washings and Crude polysaccharides merge, stirring and dissolving gets the Crude polysaccharides aqueous solution, filters, collect filtrate, filtrate is carried out ultrafiltration, nanofiltration by ultra-filtration membrane, nanofiltration membrane, collect concentrated solution, promptly.
Described concentrated solution further lyophilize gets Aloe polysaccharide dry powder.
After described circulation concentrates the Aloe gel inspissated juice is 1/20 times of original Fresh leaf of aloe juice, to enter volume/hold back volume calculation.
The present invention adds in the Aloe gel inspissated juice and staticly settles after 9 times 95% ethanol stirs, can save the alcohol consumption like this, reduce cost, other composition of Aloe polysaccharide and aloe is effectively separated, help filtering, in addition, the present invention concentrates, filters by ultra-filtration membrane and nanofiltration membrane, thus make that the present invention can effectively separate to greatest extent from asparagus juice or Aloe gel inspissated juice, extraction, purifying Aloe polysaccharide.The present invention can directly apply to the high-quality Aloe polysaccharide of batch preparations, and keeps its original activity, has the saving cost, the technology characteristic of simple.
Description of drawings:
Accompanying drawing is for being process flow sheet of the present invention.
Embodiment:
Shown in accompanying drawing, after the Fresh leaf of aloe harvesting, soak for some time with clear water, washes clean.Bubble is 15 minutes in thimerosal, and is clean with the clear water rinsing, cut the finishing of leaf sheath and blade tip after, de-epithelization is smash mesophyll and is starched into colloidal, and carries out biological pourpoint depression.Aloe gel juice behind the pour point depression through link-suspended basket centrifuge, is obtained centrifugal clear liquid, get clear liquid and pass through nanofiltration membrane again, collect concentrated solution, obtain Aloe gel inspissated juice into Aloe original juice 1/20 through ultra-filtration membrane, nanofiltration membrane through ultra-filtration membrane.The ethanol that adds 9 times 95% in the Aloe gel inspissated juice, normal temperature fully stirs down, leaves standstill 2 hours.Filter, reclaim ethanol, collect Crude polysaccharides, wash filter cloth with water 3 times, washings and Crude polysaccharides merge, and stirring and dissolving gets the Crude polysaccharides aqueous solution.Filter, collect filtrate, with filtrate by behind the ultra-filtration membrane, obtain filtrate I and concentrated solution I, with collect the nanofiltration membrane of filtrate I by aperture, nano level top layer circulate after concentrating concentrated solution II, at last concentrated solution II that is concentrated and the concentrated solution I that obtains previously mixing are obtained the Aloe polysaccharide concentrated solution, the Aloe polysaccharide concentrated solution can directly utilize, and also further lyophilize gets Aloe polysaccharide dry powder.
Its active coating of described nanofiltration membrane is a polyacrylamide, and supporting layer is a polystyrene, and fibrous reticulum is a polyester, interior pressure-pipe type membrane module, one section circulating arrangement of one-level.
Claims (3)
1, process for extracting aloinose, with the Fresh leaf of aloe of treated mistake smash starch into Folium Aloe juice after-filtration, centrifugal, circulate after concentrating the Aloe gel inspissated juice, it is characterized in that: in the Aloe gel inspissated juice, add 9 times of amount 95% ethanol, normal temperature fully stirs down, left standstill 1-3 hour, and filtered, reclaim ethanol, collect aloe coarse polysaccharid, filter cloth washes with water 3 times, and washings and Crude polysaccharides merge, stirring and dissolving, get the Crude polysaccharides aqueous solution, filter, collect filtrate, filtrate is carried out ultrafiltration, nanofiltration by ultra-filtration membrane, nanofiltration membrane, collect concentrated solution, promptly.
2, process for extracting aloinose according to claim 1 is characterized in that: described concentrated solution further lyophilize gets Aloe polysaccharide dry powder.
3, process for extracting aloinose according to claim 1 is characterized in that: after described circulation concentrates the Aloe gel inspissated juice is 1/20 times of original Fresh leaf of aloe juice, to enter volume/hold back volume calculation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02114216 CN1208348C (en) | 2002-06-26 | 2002-06-26 | Process for extracting aloinose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02114216 CN1208348C (en) | 2002-06-26 | 2002-06-26 | Process for extracting aloinose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1463987A true CN1463987A (en) | 2003-12-31 |
| CN1208348C CN1208348C (en) | 2005-06-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02114216 Expired - Fee Related CN1208348C (en) | 2002-06-26 | 2002-06-26 | Process for extracting aloinose |
Country Status (1)
| Country | Link |
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| CN (1) | CN1208348C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100491404C (en) * | 2005-10-10 | 2009-05-27 | 云南元江万绿生物（集团）有限公司 | Fractional extraction method of aloe polysaccharide |
| CN101555507B (en) * | 2009-05-18 | 2012-03-28 | 杨凌壹之农微生物工程技术研究院有限公司 | Method for using pulullan to prepare high-purity maltotriose |
| CN109971427A (en) * | 2019-04-23 | 2019-07-05 | 卢江华 | A kind of environmental protection foam formulations and preparation method thereof |
| CN113563491A (en) * | 2021-09-01 | 2021-10-29 | 重庆化工职业学院 | Extraction method of aloe polysaccharide |
| CN114605566A (en) * | 2022-03-14 | 2022-06-10 | 中国科学院长春应用化学研究所 | Method for extracting aloe gel macromolecule active ingredient and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101392033B (en) * | 2008-11-03 | 2011-12-21 | 中国烟草总公司郑州烟草研究院 | Aloe polysaccharide extraction method and use thereof in cigarette |
-
2002
- 2002-06-26 CN CN 02114216 patent/CN1208348C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100491404C (en) * | 2005-10-10 | 2009-05-27 | 云南元江万绿生物（集团）有限公司 | Fractional extraction method of aloe polysaccharide |
| CN101555507B (en) * | 2009-05-18 | 2012-03-28 | 杨凌壹之农微生物工程技术研究院有限公司 | Method for using pulullan to prepare high-purity maltotriose |
| CN109971427A (en) * | 2019-04-23 | 2019-07-05 | 卢江华 | A kind of environmental protection foam formulations and preparation method thereof |
| CN113563491A (en) * | 2021-09-01 | 2021-10-29 | 重庆化工职业学院 | Extraction method of aloe polysaccharide |
| CN114605566A (en) * | 2022-03-14 | 2022-06-10 | 中国科学院长春应用化学研究所 | Method for extracting aloe gel macromolecule active ingredient and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1208348C (en) | 2005-06-29 |
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