CN1451295A - Process for preparing enzymolysis feed for breeding shellfish - Google Patents

Process for preparing enzymolysis feed for breeding shellfish Download PDF

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Publication number
CN1451295A
CN1451295A CN03116967A CN03116967A CN1451295A CN 1451295 A CN1451295 A CN 1451295A CN 03116967 A CN03116967 A CN 03116967A CN 03116967 A CN03116967 A CN 03116967A CN 1451295 A CN1451295 A CN 1451295A
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gram
enzyme
dregs
water
shellfish
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CN03116967A
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CN1220444C (en
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叶均安
刘建新
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Zhejiang University ZJU
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Zhejiang University ZJU
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Priority to CNB031169678A priority Critical patent/CN1220444C/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

A process for preparing the enzymolyzed feed used for culturing shellfish includes soaking fish, shrimp and bean dregs or cotton seed dregs or rape seed dregs in water, mixing with a mixture, and enzymolyzing at 5-50 deg.C. Its advantages are simple operation, rich nutrients, easy being assimilated and less environmental pollution.

Description

A kind of enzymolysis bait preparation method who is used for cultivated shellfish
Technical field
The present invention relates to biological technical field, particularly, the present invention relates to a kind of enzymolysis bait preparation method who is used for cultivated shellfish, more particularly, the present invention relates to the nutritional characteristic of filter-feeding shellfish, by enzymolysis protein matter feed and additional other nutritional labeling, make it to become active bait nutritious, that can be absorbed rapidly by the filter-feeding shellfish, promote the shellfish growth, simultaneously, promote unicellular alga growth in the water, enrich shellfish bait, reduce environmental pollution, guarantee a kind of new method of shellfish healthy growth.
Background technology
In recent years, because China encloses and is coated with cultured area and enlarges year by year, coastal prawn culturing benefit descends, intertidal shellfish is cultured and is obtained large-scale development, the razor clam of particularly hanging, the mud blood clam, clam, mussel, the high density intensive culture of scallop and oyster, utilize natural zooplankter in the seawater, unicellular alga can not have been satisfied the normal growth needs of shellfish as the traditional approach of foundation bait, for promoting the growth of shellfish, the coastal culturist of China utilizes the low value fishes and shrimps directly to throw in shellfish culture through rubbing or without any processing and coats, raising together with under the situation of prawn, excreta by the shrimp body becomes shellfish bait, during monoculture, through rotting naturally, after the decomposition, as shellfish bait or be used for rich water, promote the unicellular alga growth, enrich shellfish bait.This cultural method can not be degraded rapidly because of the fishes and shrimps material when monoculture, is absorbed, utilizes by shellfish, easily causes to be coated with matter blackout, aging, harmful microorganism grows in a large number, simultaneously, produces harmful substances such as hydrogen sulfide because of fishes and shrimps are corrupt, cause shellfish anoxic, premunition to descend, even large tracts of land death; When water temperature was low, this cultural method had not both reached the purpose that promotes unicellular alga, shellfish growth, contaminated environment again.
In culturing, China's intertidal shellfish still have the fermented livestock of use excrement, vegetable protein (as soya-bean cake) fermented product, chemical industry fertilizer to splash at beach (water surface), by cultivating unicellular alga, improve the shellfish growth, but, such food nutrition composition is extremely uneven, cultivation is suitable for benthic algae limited in one's ability of shellfish growth, and is easily absorbed by macros such as blue mud tongue, hydrodictyon reticulatums, does not reach the purpose of accelerating the shellfish growth.And easily cause water pollution, be coated with the aging and shellfish of matter and fall ill because of nutritional deficiency even large tracts of land death, bring very big loss to the operator.
Summary of the invention
The objective of the invention is a kind of enzymolysis bait preparation method who is used for cultivated shellfish.
Its step is as follows:
1) at first gets portion through rubbing or expecting to add 0~3 part in water in bag or the bucket without the fresh net dilating fish that rubs, shrimp; Or get a through rubbing or expecting to add 0~6 part in water in bag or the bucket without the fresh fish that rubs, shrimp; Or get in a dry fish, shrimp med bag or the bucket and add 0.5~18 part in water; Or get in a dregs of beans or the cottonseed dregs of rice or rapeseed dregs bag or the bucket and add 0.5~18 part in water, soaked 1 day;
2) then, add 0.3~3 part pre-mixing agent, mixing, enzyme decomposes in 5~50 ℃ environment, stirs every day or stirs 1~6 time; When environment temperature was lower than 20 ℃, enzyme resolving time was at 1~10 day, and environment temperature is in the time of 20~35 ℃, and enzyme resolving time was at 8 hours~5 days; Environment temperature is when being higher than 35 ℃, and enzyme resolving time was at 4 hours~3 days, and with the increase of enzyme resolving time, thinning gradually, easily mobile, it is black that color deepens gradually, becomes, and after extremely integral body becomes stable pitchy, placed 1~2 day again, can use.
The present invention be with the protein feeds raw material through enzymolysis, become small-molecular peptides, amino acid and the monose that can be absorbed rapidly by shellfish, bait feeding, shellfish growth are not subjected to the influence of weather, water temperature.Little peptide, amino acid and metallic element complexing that enzymolysis generates generate organic salt, help the conversion that shellfish absorbs and body is interior, promote the shellfish growth.Reduce the environmental pollution that feeds intake and cause, promote the unicellular alga growth, reach the sustainable development of shellfish culture.
The present invention is easy and simple to handle, and the active bait of production is nutritious, and can be absorbed rapidly by shellfish, promotes the growth of shellfish; Simultaneously, can promote the growth of unicellular alga, enrich bait, reduce the pollution of bait environment for shellfish provides.
The specific embodiment
A kind of pre-mixing agent that is used for the enzymolysis bait preparation method of cultivated shellfish, during with fishes and shrimps protein feeds source, by weight percentage, protease 3~30%, mineral salt 5~59%, vitamin 0.02~0.5%, anticorrisive agent 5~10%, diluent 86.98~0%, the said protease that contains, its enzyme activity is 30~3,000,000 units, contains mineral trace element: iron content is 2~20 grams, zinc content is 0.3~5 gram, manganese is 0.5~5 gram, copper is 0.05~0.5 gram, cobalt 0.001~0.02 gram, selenium 0.003~0.06 gram contains the B family vitamin: thiamine 1~25mg, riboflavin 4~100mg, nicotinic acid 20~500mg, calcium pantothenate 4~100mg, Cobastab 64~100mg, Cobastab 120.01~0.25mg, biotin 0.04~1.0mg and folic acid 0.5~10mg.
During with plant-derived protein feed sources such as dregs of beans, the cottonseed dregs of rice and rapeseed dregs, by weight percentage, protease 1.5~15%, amylase 6.25~43.75%, mineral salt 2.5~29.5%, vitamin 0.01~0.25%, anticorrisive agent 2.5~5%, diluent 87.24~6.5%, the said protease that contains, its enzyme activity are 30~3,000,000 units, starch-containing enzyme, its enzyme activity is 10~700,000 units
Contain mineral trace element: iron content is that 2~20 grams, zinc content are that 0.3~5 gram, manganese are that 0.5~5 gram, copper are 0.05~0.5 gram, cobalt 0.001~0.02 gram, selenium 0.003~0.06 gram, contains the B family vitamin: thiamine 1~25mg, riboflavin 4~100mg, nicotinic acid 20~500mg, calcium pantothenate 4~100mg, Cobastab 64~100mg, Cobastab 120.01~0.25mg, biotin 0.04~1.0mg and folic acid 0.5~10mg.
The relational language that relates among the present invention:
1, the shellfish that relates among the present invention is meant the filter-feeding shellfish, and it comprises: mud blood clam, the razor clam of hanging, clam, mussel, scallop and oyster;
2, shorten with the rising of environment temperature the standing time that relates among the present invention in 5~50 ℃ of scopes, and in 10 days, standing time is of a specified duration more, and the active bait quality of processing is good more.In the active bait process, increase and stir number of times, can shorten the process time of active bait;
3, can take the circumstances into consideration to splash again after the water mixing during fabricated product stiff among the present invention;
4, the pre-mixing agent that relates among the present invention comprises: enzyme, mineral matter element, vitamin and anticorrisive agent, the preparation through fully mixing.
(1) this pre-mixing agent is even matter powder;
(2) contain protease;
When (3) processing active bait with plant-derived protein feedstuffs such as dregs of beans, the cottonseed dregs of rice, rapeseed dregs, starch-containing enzyme in the additive;
(4) contain mineral trace element: the water soluble salt of iron, zinc, manganese, copper, cobalt, selenium;
(5) contain the B family vitamin: thiamine, riboflavin, nicotinic acid, calcium pantothenate, vitamin B6, cobalamin, biotin and folic acid;
(6) contain the anticorrisive agent that suppresses bacterium, conk;
(7) with zeolite powder as diluent.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.
Embodiment 1, with net dilating fish shrimp material (water content is more than 80%) processing shellfish active bait
Get about 15kg net dilating fish shrimp material and rub through pulverizer, put in the bucket, add water 5kg, add the pre-mixing agent portion among the present invention, mixing is added a cover, and the time stirred 1 time every 4 hours by day, when temperature during 15 ℃ of left and right sides, places 6 days; When temperature during, placed 4 days 25 ℃ of left and right sides; When temperature during 40 ℃ of left and right sides, placed 2 days, can use.Be used for the fertile Becquerel water surface that Yu Yangbei is coated with of directly splashing, according to the growth needs of shellfish, consumption is not limit; Being used for rich water should be when fine, and above-mentioned active bait can be splashed on 1 mu of water surface, or looks the water quality girth of a garment and take the circumstances into consideration increase and decrease.
Embodiment 2, with fresh or freezing fishes and shrimps material (water content is more than 60%) processing shellfish active bait
Get the fresh fishes and shrimps material of about 10kg and rub through pulverizer, put in the bucket, add water 10kg, add the pre-mixing agent portion among the present invention, mixing is added a cover, and the time stirred 1 time every 4 hours by day, when temperature during 15 ℃ of left and right sides, places 6 days; When temperature during, placed 4 days 25 ℃ of left and right sides; When temperature during 40 ℃ of left and right sides, placed 2 days, can use.Be used for the fertile Becquerel water surface that Yu Yangbei is coated with of directly splashing, according to the growth needs of shellfish, consumption is not limit; Being used for rich water should be when fine, and above-mentioned active bait can be splashed on 1 mu of water surface, or looks the water quality girth of a garment and take the circumstances into consideration increase and decrease.
Embodiment 3, with fish meal (water content is more than 10%) processing shellfish active bait
Get about 3kg fish meal, put in the bucket, add water 15kg, soak after 1 day the pre-mixing agent portion that adds among the present invention, mixing is added a cover, and the time stirred 1 time every 4 hours by day, when temperature during 15 ℃ of left and right sides, places 6 days; When temperature during, placed 4 days 25 ℃ of left and right sides; When temperature during 40 ℃ of left and right sides, placed 2 days, can use.Be used for the fertile Becquerel water surface that Yu Yangbei is coated with of directly splashing, according to the growth needs of shellfish, consumption is not limit; Being used for rich water should be when fine, and above-mentioned active bait can be splashed on 1 mu of water surface, or looks the water quality girth of a garment and take the circumstances into consideration increase and decrease.
Embodiment 4, with dregs of beans (water content is more than 10%) processing shellfish active bait
Get about 4kg dregs of beans, put in the bucket, add water 15kg, soak after 1 day the pre-mixing agent portion that adds among the present invention, mixing is added a cover, and the time stirred 1 time every 4 hours by day, when temperature during 15 ℃ of left and right sides, places 6 days; When temperature during, placed 4 days 25 ℃ of left and right sides; When temperature during 40 ℃ of left and right sides, placed 2 days, can use.Be used for the fertile Becquerel water surface that Yu Yangbei is coated with of directly splashing, according to the growth needs of shellfish, consumption is not limit; Being used for rich water should be when fine, and above-mentioned active bait can be splashed on 1 mu of water surface, or looks the water quality girth of a garment and take the circumstances into consideration increase and decrease.

Claims (3)

1. enzymolysis bait preparation method who is used for cultivated shellfish is characterized in that its step is as follows:
1) at first gets portion through rubbing or expecting to add 0~3 part in water in bag or the bucket without the fresh net dilating fish that rubs, shrimp; Or get a through rubbing or expecting to add 0~6 part in water in bag or the bucket without the fresh fish that rubs, shrimp; Or get in a dry fish, shrimp med bag or the bucket and add 0.5~18 part in water; Or get in a dregs of beans or the cottonseed dregs of rice or rapeseed dregs bag or the bucket and add 0.5~18 part in water, soaked 1 day;
2) then, add 0.3~3 part pre-mixing agent, mixing, enzyme decomposes in 5~50 ℃ environment, stirs every day or stirs 1~6 time; When environment temperature was lower than 20 ℃, enzyme resolving time was at 1~10 day, and environment temperature is in the time of 20~35 ℃, and enzyme resolving time was at 8 hours~5 days; Environment temperature is when being higher than 35 ℃, and enzyme resolving time was at 4 hours~3 days, and with the increase of enzyme resolving time, thinning gradually, easily mobile, it is black that color deepens gradually, becomes, and after extremely integral body becomes stable pitchy, placed 1~2 day again, can use.
2. a kind of enzymolysis bait preparation method who is used for cultivated shellfish according to claim 1, it is characterized in that said pre-mixing agent, it is characterized in that, during with fishes and shrimps protein feeds source, by weight percentage, protease 3~30%, mineral salt 5~59%, vitamin 0.02~0.5%, anticorrisive agent 5~10%, diluent 86.98~0%, the said protease that contains, its enzyme activity is 30~3,000,000 units, contains mineral trace element: iron content is 2~20 grams, zinc content is 0.3~5 gram, manganese is 0.5~5 gram, copper is 0.05~0.5 gram, cobalt 0.001~0.02 gram, selenium 0.003~0.06 gram contains the B family vitamin: thiamine 1~25mg, riboflavin 4~100mg, nicotinic acid 20~500mg, calcium pantothenate 4~100mg, Cobastab 64~100mg, Cobastab 120.01~0.25mg, biotin 0.04~1.0mg and folic acid 0.5~10mg.
3. a kind of enzymolysis bait preparation method who is used for cultivated shellfish according to claim 1, it is characterized in that said pre-mixing agent, use dregs of beans, during plant-derived protein feed sources such as the cottonseed dregs of rice and rapeseed dregs, by weight percentage, protease 1.5~15% amylase 6.25~43.75%, mineral salt 2.5~29.5%, vitamin 0.01~0.25%, anticorrisive agent 2.5~5%, diluent 87.24~6.5%, the said protease that contains, its enzyme activity is 30~3,000,000 units, starch-containing enzyme, its enzyme activity is 10~700,000 units, contain mineral trace element: iron content is 2~20 grams, zinc content is 0.3~5 gram, manganese is 0.5~5 gram, copper is 0.05~0.5 gram, cobalt 0.001~0.02 gram, selenium 0.003~0.06 gram contains the B family vitamin: thiamine 1~25mg, riboflavin 4~100mg, nicotinic acid 20~500mg, calcium pantothenate 4~100mg, Cobastab 64~100mg, Cobastab 120.01~0.25mg, biotin 0.04~1.0mg and folic acid 0.5~10mg.
CNB031169678A 2003-05-13 2003-05-13 Process for preparing enzymolysis feed for breeding shellfish Expired - Fee Related CN1220444C (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100398001C (en) * 2004-05-17 2008-07-02 浙江大学 Bait with enzymolysis being needed before use for shellfish
CN101912050A (en) * 2010-07-13 2010-12-15 大连海洋大学 Micro-granulated feed for shellfish and production technique thereof
CN103461225A (en) * 2013-07-30 2013-12-25 仙桃市强农水产专业合作社 Artificial bionic method for breeding rice field eel fries in net cages

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100398001C (en) * 2004-05-17 2008-07-02 浙江大学 Bait with enzymolysis being needed before use for shellfish
CN101912050A (en) * 2010-07-13 2010-12-15 大连海洋大学 Micro-granulated feed for shellfish and production technique thereof
CN101912050B (en) * 2010-07-13 2014-11-12 大连海洋大学 Micro-granulated feed for shellfish and production technique thereof
CN103461225A (en) * 2013-07-30 2013-12-25 仙桃市强农水产专业合作社 Artificial bionic method for breeding rice field eel fries in net cages

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