CN1424101A - Chinese medicines for treating dymenorrhea - Google Patents

Abstract

A Chinese medicine for treating menalgia is prepared from 4 Chinese-medicinal materials including chuan-xiong rhizome (1-5 wt portions), white peony root immersed in wine (1-5), charred argyi leaf (1-5) and corydalis tuber (1-5). Its advnatage is high curative effect.

Description

A kind of Chinese medicine for the treatment of dysmenorrhea

Technical field

The present invention relates to a kind of medicine, particularly relate to a kind of medicine for the treatment of dysmenorrhea and preparation method thereof.

Background technology

The dysmenorrhea that female incretion imbalance causes is one of world today's medical science difficult problem of not capturing as yet.Introduce in " female chronic pelvic pain " book, the Britain women of child-bearing age 12% have serious dysmenorrhea, and 45% has moderate to the severe pain warp.(English) periodical report U.S. has among the women of periodically menstruation 90% dysmenorrhea is arranged, and 36% has dysmenorrhea always or often.Point out that according to Britain one tame authorities,medical mechanism investigation report as seen 80% dysmenorrhea that has in various degree among the global women exists number many, the somebody slightly restrains oneself a bit, can be as life at ordinary times, yet the somebody but pain can not stand.Gynaecologist doctor Kennedy of Oxford University says in the meeting of Britain scientific achievement association: " 2/3rds women suffer from dysmenorrhoea, and 3/4ths sick sending out can't be worked." China's woman month dysmenorrhea person in menstrual period accounts for 33.19%, slightly accounts for 45.73%, moderate accounts for 38.81%, and severe accounts for 13.55%.China's women's population has 60,189 ten thousand people, wherein reproduction age population 34,185 ten thousand people, calculate by the minimum sickness rate of dysmenorrhea 33.19%, dysmenorrhea patient just has 11,346 ten thousand people, not only sickness rate height but also patient are stable., external dysmenorrhea incidence rate 43-90% is much higher than domestic.Up to the present, because each one pain sensation threshold value difference, tolerance degree is very big, and lacks the objective quantitative approach of accurately measuring pain degree, and is very big to the report difference of dysmenorrhea sickness rate both at home and abroad.Among 71746 women of China's menstrual physiology constant cooperative groups investigation in 1980,33.2% has dysmenorrhea.Its Central Plains is sore through 36.1%, secondary dysmenorrhea 31.7%, unknown cause 32.2%.Severe pain is through influence life and worker 13.6%.Among 19 years old city young woman of nineteen eighty-two Andersch and Milsom report Sweden, 72% has dysmenorrhea, wherein 15% needs the clothes analgesic.About 50% dysmenorrheas that have in various degree among the women were arranged after the report U.S. adolescence in 1985, and 10% needed to have a rest 1～3 day because of dysmenorrhea in every month.This shows that dysmenorrhea is the disease that takes place for more generally in women's illness.In recent years report abroad that women's dysmenorrhea incidence rate before 19 years old obviously increases.

Summary of the invention

The object of the invention is to provide a kind of medicine for the treatment of dysmenorrhea and preparation method thereof.

The present invention seeks to be achieved through the following technical solutions:

Crude drug is by weight: Rhizoma Chuanxiong 1-5 part Radix Paeoniae Alba 1-5 part charred Folium Artemisiae Argyi 1-5 part Rhizoma Corydalis 1-5 part

Preferably be provided as: 4 parts of 4 parts of Rhizoma Corydalis of 3 parts of charred Folium Artemisiae Argyis of 3 parts of Radix Paeoniae Alba of Rhizoma Chuanxiong

The above-mentioned raw materials medicine can be made any clinical acceptable forms, as tablet, and capsule, granule, oral liquid, subcutaneous administration preparation, suppository etc.

The preparation method of medicine of the present invention: 4 flavor decoctings such as Rhizoma Chuanxiong 1-3 time, add 8-13 times of water gaging at every turn, decocted 12 hours, filter; Merge decocting liquid, be evaporated to relative density and be 1.08-1.14/60 ℃ clear paste, adding ethanol is 50-70% to alcohol precipitation concentration, and placement is spent the night; Filter, decompression recycling ethanol is to relative density concentration 1.08-1.14; Spray drying is made clinical acceptable forms, as tablet, and capsule, granule, oral liquid, subcutaneous administration preparation, suppository etc.

Medicine main pharmacodynamics result of study of the present invention has confirmed that this medicine has stronger treatment dysmenorrhea effect.

Be subjected to the reagent thing: this medicine, provide by the Drug Manufacturing Room of China Academy Of Traditional Chinese Medicine Traditional Chinese Medicine Research Institute, contain 8.8g crude drug/g.Laboratory animal: the Wistar rat, female, body weight 160 ± 10g; Kunming mouse, female, body weight 21 ± 1g; Rabbit, female, body weight 2-2.5kg.Dosage design: the clinical consumption of people is 28g crude drug/50kg/d, both 0.56g/kg/d, during experiment the mice consumption be 12,6,3g crude drug/kg/d, press the 0.2ml/10g gastric infusion; The rat consumption is 6,3,1.5g crude drug/kg/d, presses the 2ml/100g gastric infusion; The rabbit consumption is 5,2.5,1.25g crude drug/kg/d, press the 1ml/kg gastric infusion respectively quite with 2 times of people's consumption, etc. times and 1/2 times.

The experiment grouping: 10 of negative control group (male), 10 of model control group, 10 of positive drug control group, 10 of the heavy dose of groups of administration, the dosage group is 10 in the administration, 10 of administration small dose group Experimental example one,The influence of primary dysmenorrhea model

1. to the influence of oxytocin induced mice hysterospasm: get 60 of Kunming mouses, be divided into above 6 groups, each Corium Mus down injection diethylstilbestrol 0.2mg/ only, for three days on end.Administration in the 4th day, the typical case is only taken place and is turned round the Mus number of body and turn round the body number in each Mus lumbar injection oxytocin 0.2u/ after 1 hour in 30 minutes behind the observation oxytocin injection.Compare the T check between employing group as a result and carry out statistical procedures.

2. to the influence of rat uterus spasm due to the PGE2a: get 60 of Wistar rats, be divided into above 6 groups, except that negative control group, each Corium Mus is the injection diethylstilbestrol down, continuous 4 days, dosage is respectively 0.8,0.4,0.4,0.8mg/ only, the while gastric infusion, continuous 5 days, the typical case was only taken place and is turned round the Mus number of body and turn round the body number in 1 hour each Mus lumbar injection PGE2a 1mg/ after the last administration in 30 minutes behind the observation injection PGE2a.Compare the T check between employing group as a result and carry out statistical procedures.

After learning processing by statistics, confirm that medicine of the present invention has significant mitigation to the uterine smooth muscle spasticity of above-mentioned 2 models of experimental rat/mice " primary dysmenorrhea ".Suppression ratio is 50%. Experimental example two,To the influence of rabbit in the body uterine contractility

1. to the influence of normal uterine contractility: test every rabbit intramuscular injection diethylstilbestrol 1mg/kg of beginning in preceding 2 days, for three days on end.Animal is used the intravenous anesthesia of pentobarbital sodium 30mg/kg body weight during experiment.Face upward the position and be fixed in operating-table, the hypogastric region otch exposes the uterus, selects to be about a section of 3cm at one side cornua uteri, and mid point and the muscular tension transducer two ends that link are fixed on the fulcrum of both sides, glass infuser bottom.Place locke solution in the glass infuser, the operating lamp insulation.Find out duodenum in addition and be used for administration.Close the abdominal cavity.Trace uterine activity before the administration after stable, duodenal administration is then traced 30,60 minutes uterine activities after the administration.

2. to the influence of uterine contractility due to the oxytocin: every rabbit intramuscular injection diethylstilbestrol 1mg/kg before the experiment, for three days on end.Respectively organize simultaneously gastric infusion, during experiment in the 4th day, animal is used the intravenous anesthesia of pentobarbital sodium 30mg/kg body weight.Face upward the position and be fixed in operating-table, the hypogastric region otch exposes the uterus, selects to be about a section of 3cm at one side cornua uteri, and mid point and the muscular tension transducer two ends that link are fixed on the fulcrum of both sides, glass infuser bottom.Place locke solution in the glass infuser, the operating lamp insulation.Duodenal administration once in addition.Close the abdominal cavity.Trace uterine activity in 30 minutes behind the medicine, in locke solution, splash into oxytocin then, uterine activity when tracing 30,60 minutes.Statistical procedures is carried out in comparison T check and difference t check between result's employing group respectively.

After learning processing by statistics, confirm that medicine of the present invention shrinks the nothing influence to experimental rabbit (at body) normal uterus, the uterine contraction state that oxytocin is caused has mitigation significantly. Experimental example three,Influence to the influence of uterus, the development of ovary and estrogen level

1. to the influence of normal rat: get 50 of rats, female, body weight 160=10g, be divided into 5 groups at random, administration group gastric infusion is dissected animal after continuous 15 days, estrogen is surveyed in blood sampling, take by weighing uterus, ovary, calculating corresponding organs index compares the T check between result's employing group respectively and statistical procedures is carried out in index t check.

2. to the influence of castrated rats: get 50 of rats, female, body weight 160=10g, anesthesia back surgical removal of ovaries is divided into 5 groups at random, and administration group gastric infusion is dissected animal after continuous 15 days, and estrogen is surveyed in blood sampling, takes by weighing the uterus, calculates the corresponding organs index.Statistical procedures is carried out in comparison T check and index t check between result's employing group respectively.

After learning processing by statistics, the uterus development of ovary does not make significant difference to the castration experimental rat to confirm medicine of the present invention, and there is certain promotion in the normal experimental rat uterus and the development of ovary, and the rat estrogen level is not had obvious influence.Meet the requirement of dysmenorrhea pharmacodynamic study. Experimental example four,Analgesic effect:

50 of mices are divided into 5 groups at random by body weight, gastric infusion of each administration group, 0.2ml/10g after 1 hour, the acetic acid 0.2ml/ of lumbar injection 0.5% only, observe the typical number of times of turning round body of each mice appearance in 20 minutes, compare the t check between employing group as a result and carry out statistical procedures.Experimental results show that this medicine has tangible analgesic effect to mice.

Embodiment:Rhizoma Chuanxiong 660g, Radix Paeoniae Alba 660g, charred Folium Artemisiae Argyi 880g, Rhizoma Corydalis 880g, decocting 2 times adds 12 times of water gagings at every turn, decocts 1 hour, filters; Merge decocting liquid, be evaporated to relative density and be 1.08-1.14/60 ℃ clear paste, adding ethanol is 60% to alcohol precipitation concentration, and placement is spent the night; Filter, decompression recycling ethanol is to relative density concentration 1.08-1.14.

Add the 20g beta-schardinger dextrin-, mixing, spray drying; Extract powder adds beta-schardinger dextrin-to 350g, adds the 2g magnesium stearate, mix homogeneously; Press dry granulation, make 1000 No. 1 capsules.Every heavy 0.35 gram, every gram extract contains crude drug 8.8 grams.

Claims (5)

1. medicine for the treatment of dysmenorrhea is characterized in that this medicine made by following crude drug:

Crude drug is by weight: Rhizoma Chuanxiong 1-5 part Radix Paeoniae Alba 1-5 part charred Folium Artemisiae Argyi 1-5 part Rhizoma Corydalis 1-5 part.

2. a kind of medicine for the treatment of dysmenorrhea as claimed in claim 1 is characterized in that this medicine made by following crude drug: 3 parts of Rhizoma Chuanxiongs; 3 parts of Radix Paeoniae Alba; 4 parts of charred Folium Artemisiae Argyis; 4 parts of Rhizoma Corydalis.

3. a kind of medicine for the treatment of dysmenorrhea as claimed in claim 1 or 2 is characterized in that this medicine material medicine can make any clinical acceptable forms, as tablet, and capsule, granule, oral liquid, subcutaneous administration preparation, suppository.

4. a kind of medicine for the treatment of dysmenorrhea as claimed in claim 3 is characterized in that the preparation method of this medicine is:

4 flavor decoctings such as Rhizoma Chuanxiong 1-3 time add 8-13 times of water gaging at every turn, decoct 1-2 hour, filter; Merge decocting liquid, be evaporated to relative density and be 1.08-1.14/60 ℃ clear paste, adding ethanol is 50-70% to alcohol precipitation concentration, and placement is spent the night; Filter, decompression recycling ethanol is to relative density concentration 1.08-1.14; Spray drying is made clinical acceptable forms, as tablet, and capsule, granule, oral liquid, subcutaneous administration preparation, suppository.

5. a kind of treatment dysmenorrhea medicine as claimed in claim 4 is characterized in that the preparation method of this medicine is:

4 flavor decoctings such as Rhizoma Chuanxiong 2 times add 12 times of water gagings at every turn, decoct 1 hour, filter; Merge decocting liquid, be evaporated to relative density and be 1.08-1.14/60 ℃ clear paste, adding ethanol is 60% to alcohol precipitation concentration, and placement is spent the night; Filter, decompression recycling ethanol is to relative density concentration 1.08-1.14; Add 20 weight portion beta-schardinger dextrin-s, mixing, spray drying; Extract powder adds beta-schardinger dextrin-to 350 weight portion, adds 2 weight portion magnesium stearate, mix homogeneously; Press dry granulation, incapsulate.