Summary of the invention
The objective of the invention is to overcome above-mentioned the deficiencies in the prior art part, and easily manufactured, effective peptide milk powder (trade name: sunrise is peptide milk powder too) and preparation technology thereof is provided.
The object of the invention can realize by following measure:
Peptide milk powder is to be made by following raw material and prescription:
Plain chocolate 94-96%
White granulated sugar 2-3%
Bone marrow peptide 2-4%
The content sum of above each component percentage by weight is 100%, water dissolved sugar and marrow powder, water and contain a little soda ash and all ignore.
The technology of preparation peptide milk powder, as follows:
1, milk collection
Regain fresh milk from the pasture, through outgasing, filter, cool off, go into milk-storage tank.
2, give processing
1) adjustment of marrow powder pH value
Accurately metering marrow powder is used the hot water dissolving, and it is 7.0 that soda ash liquid is adjusted pH value, and cooling is left standstill.
2) pure milk gives processing
The pure milk of refrigeration is squeezed into compensating groove, heating, centrifugal, squeeze into allotment.
3) allotment of peptide milk
With the dissolving of granulated sugar water, add in the milk with peptide liquid, stir.
4) concentrate
The peptide milk liquid for preparing vacuum after filtration concentrates.
5) spraying
Dense milk temperature is at 45-50 ℃, and powder is cooled off, sieved to spray-drying, packing.
Bone marrow peptide is the biologically active of extracting from cattle and sheep fresh bone meat tissue, is had " regulating immunity " health care foods by health ministry approval production in 1997.This active peptide product is added in the milk, and the peptide milk powder of making has immunoregulatory effect undoubtedly.
Bone marrow peptide opaque amount standard sees the following form:
The bone marrow peptide sample is delivered to nutrition and food hygiene teaching and research room of Beijing Medical University, and this teaching and research room has made health food immunoregulation effect survey report, now assay is presented below: test rating:
Spleen of mouse and thymus gland are heavy; Antibody forming cell's (PFC) mensuration improves slide method with Jerne; Delayed allergy (DTH) is measured with the pedal swelling method; The detection of macrophage phagocytic function adopts the carbon particle clearance method to measure statistical method: with two groups of mean checks.Animal used as test:
Kunming kind male and female mouse, age in 6-8 week, 18-22 gram, scientific experiment animal portion of Beijing Medical University provides, and the quality certification number is: the moving word 01-3049 of doctor.Experimental design:
Animal is divided control group, low dose group, middle dosage group and high dose group.The dosage of above-mentioned three experimental group is respectively 0.25g/Kg, 1.0g/Kg and 3.0g/Kg body weight.Be equivalent to 2.5,10 and 30 times of people's consumption every day respectively, the continuous irrigation stomach is 14 days altogether.Measurement result:
1, thymic weight of each dosage group mouse and control group more all have tangible increase, and mouse spleen weight and control group are not seen significant change (table 1).
2, middle and high dosage group mouse IgM-PFC number/full spleen and apparent in view increase (table 2) of control group.
3, the delayed allergy of high dose group mouse (DTH) relatively has obvious enhancing with control group, and low, middle dosage group and control group are not seen significant change (table 3).
4, each dosage group mouse carbon particle clearance rate (K) and clean up index (a) and control group and do not see significant change (table 4).
Table 1 bone marrow peptide is to mouse immune organ weight's influence (X ± S)
Group | n | Spleen phase counterweight | Thymus gland phase counterweight |
Solvent control | 12 | ?5.82±1.31 | ?3.70±0.68 |
Low dose group | 12 | ?5.81±1.56 | ?4.58±1.10* |
Middle dosage group | 12 | ?5.71±1.13 | ?4.54±1.27* |
High dose group | 12 | ?5.54±1.16 | ?4.48±1.02* |
Heavy (the mg)/body weight of phase counterweight=internal organs (g) compare with control group
*P<0.05
Table 2 bone marrow peptide is to the influence of mouse IgM-PFC/ spleen
Compare with control group
*P<0.01
Table 3 bone marrow peptide is to the influence of mouse DTH reaction (X ± S)
Group | n | The foot sole of the foot increases thickness (mm) |
Solvent control | 12 | ?0.52±0.14 |
Low dose group | 12 | ?0.52±0.17 |
Middle dosage group | 12 | ?0.63±0.18 |
High dose group | 12 | ?0.66±0.18* |
Compare with control group
*P<0.05
Table 4 bone marrow peptide is to the influence of mouse carbon particle clearance (X ± S)
Group | ??n | ??K | ??α |
Solvent control | ??11 | ??0.0436±0.0180 | ??6.80±0.77 |
Low dose group | ??11 | ??0.0471±0.0077 | ??6.94±0.62 |
Middle dosage group | ??11 | ??0.0559±0.0202 | ??6.88±0.73 |
High dose group | ??12 | ??0.0380±0.0082 | ??6.81±0.49 |
Brief summary as a result:
Its mouse oral gave bone marrow peptide after 14 days, and testing result shows that bone marrow peptide is significantly increased mouse thymus weight, strengthened the effect of humoral immune function (IgM-PFC) and cellular immune function (DTH), and not seeing significantly influences macrophage phagocytic function.
The key problem in technology point is the pH value of peptide
The final pH value of peptide powder with zymolysis technique production is 6.0, and the pH value of fresh milk is generally 6.5~6.8.If directly the peptide powder being mixed with solution adds in the pure milk, will destroy the balance of the original system of fresh milk, the result is that lamination appears in liquid peptide milk; If make peptide milk powder, after molten, the dough of floating a large amount of indissolubles on the liquid level, after the placement room temperature, the cup end, form white precipitate.If peptide liquid pH value is adjusted to the pH value identical with fresh milk, this phenomenon still exists.In order to address this problem, liquid towards milk has adopted a lot of measures, finds the binding site that combines the best of peptide and milk finally, this point is exactly a pH value 7.0, peptide liquid pH value is adjusted to 7.0, add in the pure milk, neither influence the stability that taste does not influence whole mixed system.
The present invention's milk compared to existing technology has following advantage: product of the present invention promotes cell metabolism to people's physical efficiency regulatory function, and statocyte nutrition improves body immunity, promotes the absorption of milk constituents.
The specific embodiment
Enumerate an embodiment below, the present invention is further specified.
Embodiment 1: it is as follows to fill a prescription:
The raw material kilogram
Plain chocolate 945
White granulated sugar 25
Marrow powder 30
Add a little soda ash, more than four raw material total amounts be 1000 kilograms.
With the fresh milk that regain in the pasture, pour compensating groove into, through outgasing, filtering, be cooled to 0-4 ℃, squeeze into milk-storage tank, processing is given in refrigeration, the marrow powder is accurately measured 30 kilograms, with 60 ℃ of water-soluble separating, transfer pH value to 7.0 with soda ash, be cooled to below 10 ℃, leave standstill 10 hours stand-by.The plain chocolate of refrigeration is squeezed into compensating groove for 945 kilograms, be heated to 35-40 ℃ by plate type heat exchanger, allotment is squeezed in centrifugal cooling, the allotment of peptide milk: with 25 kilograms of dissolvings of granulated sugar, add in the cow's milk with the marrow powder, stir.The peptide milk for preparing is concentrated into 12-16mmHg/50 ℃ through vacuum, and dense milk temperature can be carried out spray-drying immediately at 45-50 ℃, again through cooling off, sieve powder, being packaged into peptide milk powder.