CN1415211A - Bait formulation of fungi - Google Patents
Bait formulation of fungi Download PDFInfo
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- CN1415211A CN1415211A CN 02156520 CN02156520A CN1415211A CN 1415211 A CN1415211 A CN 1415211A CN 02156520 CN02156520 CN 02156520 CN 02156520 A CN02156520 A CN 02156520A CN 1415211 A CN1415211 A CN 1415211A
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- fungi
- bait formulation
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Abstract
A fungus bait is composed of fungus spore (1-20 wt.%), mineral carrier (0-30%), plant oil (10-25%), sugar (1-15%), the solvent for extracting sex hormone and aggregative hormone of cockroach (0.1%), and plant carrier (rest).
Description
Technical field
The present invention relates to a kind of Bait formulation of fungi that is used to prevent and treat cockroach.
Background technology
Cockroach is the serious class room sanitary insect pest of harm during people live.In China kind more than 200 is arranged, from south to north, all have distribution, kind to comprise large-scale American cockroach, Australian cockroach, Japan big Lian, the big Lian of foxiness and small-sized Groton bug etc.Along with improving constantly of living standard, interior decoration provides good sheltered situation and breeding place for cockroach, and its harm also is on the rise.Studies have shown that multiple causal organisms such as its portability bacterium, fungi, virus, parasitic ovum (Aspergillus flavus, polyovirus, the hepatitis B of Mycobacterium leprae, carcinogenic and teratogenesis toxicity); When movable, void excreta at any time and vomitus, and secrete unpleasant smelly liquid, can cause bad reactions such as people, pet are felt sick, vomiting, allergy with the belly scent gland.Also can creep into household electrical appliances inside such as computer, television set, refrigerator, smoke exhaust ventilator, cause burning parts, all can cause serious harm causes such as traffic, national defence, weaving, food, pharmacy, electronics, tourisms.
The room is the main place of human survival activity; the protection living environment; realize that green living is the dream that the modern lives; improper being suitable for of chemistry agricultural made people produce huge worry; and hygienic insecticide is directly used in daily life, working environment; use biologic product free from environmental pollution control sanitary insect pest, people's life, health is all had great importance.
Green muscardine fungus is the fungi that a class can infect insect, for Deuteromycotina Hyphomycetes green muscardine fungus belongs to.SnebB. (1991) at first the screening activity infect the green muscardine fungus bacterial strain of cockroach, registered the product of first green muscardine fungus control cockroach at U.S. Register in 1994, and regarded as environment-friendly products by American National Environmental Protection Agency.
CN93117629 (the application people is U.S. Ecoscience Corporation, August 19 1993 applying date) discloses green muscardine fungus has lethal effect to termite, can use control termites.
Although fungal spore can be made into pulvis, finish, pulvis uses duster or manually spreads fertilizer over the fields or mix pesticide-clay mixture, can not convert water spray, requirement is used under calm condition, the shortcoming of drift contaminated environment is arranged, bring into play, generally not as liquid formulation from active ingredient distributing homogeneity and drug effect.Finish is convenient to store and transportation, but must use ultra-low-volume sprayer to spray, and the spraying apparatus is had strict demand, therefore, has limited the scope of application of this formulation.
Disinsection fungal has been made into various formulations, but does not have bait formulation, because existing traditional theory thinks that insect pathogenic bacteria all invades from the insect body wall epidermis.Numerous researchs parcel has all been set forth invasion procedure, and promptly spore adheres to epidermis, sprouting, traditional body wall under the effect of enzyme then, and in the pin main body internal breeding, cause a disease.Swallowed by insect even there are some researches show the conidium of green muscardine fungus or white muscardine fungi, in insect gut, can not sprout and cause a disease.Fungal spore can not enter in the insect bodies by digestive tract, so it is necessarily very poor to make the bait formulation control efficiency.
Summary of the invention
But experimental studies have found that through the inventor, improve the bait formulation prescription, fungal spore is entered in the insect bodies by digestive tract, thereby arrive the purpose of control insect.In addition habit and the living environment of a lot of insects are discovered that bait formulation is the formulation that is suitable for some insect.One of unsettled reason of fungi preparation control efficiency is that disinsection fungal can not initiatively be sought the host, if fungal spore did not touch insect after fungi preparation was used a period of time, spore loses infecting potential probably under the influence of external environment.So allowing insect look for fungi as early as possible is the effective ways of control efficiency, is about to fungi preparation and makes bait formulation.Therefore, the purpose of this invention is to provide a kind of new Bait formulation of fungi.
One embodiment of the invention provide a kind of Bait formulation of fungi, it is characterized in that described glue bait is by fungal spore, mineral carrier, vegetable oil, sugar, cockroach sex hormone with assemble that hormone extracts solvent and the vegetalitas carrier is formed.
Another embodiment of the present invention provides by (percentage by weight) fungal spore 1%-20%, mineral carrier 0-30%, vegetable oil 10%-25%, sugared 1%-15%, cockroach sex hormone and assembles hormone and extract solvent 0.1%, the Bait formulation of fungi that surplus is formed for the vegetalitas carrier.
Fungal spore used in the Bait formulation of fungi of the present invention can be selected from green muscardine fungus, preferred Metarhizium anisopliae IBCCM321.93, this bacterial strain has been deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on December 12nd, 2002, preserving number is CGMCC No.0853, or white muscardine fungi and other have the fungi of pathogenic effects to cockroach.Used fungal spore is generally pure conidial powder, and purity is greater than 95%, and water content is less than 10%.
Described vegetalitas carrier is to be selected from one or more following materials to make: corncob, rice husk, cracker meal, rusk powder, wheat bran and stalk.Its effect is the too much oil of filling, adsorbing spores, absorption, lure and get food, adapt to cockroach and get food.Usually to use the known various machineries-pulverizing of prior art, described vegetalitas carrier is processed into the mixture of forming by the grain and the powder of following size and ratio: bar-shaped grain, length 1.95 ± 0.32mm, diameter 0.66 ± 0.21mm accounts for 27% of total vegetalitas carrier; The sheet grain, length 0.97 ± 0.27mm accounts for 14% of total vegetalitas carrier; Powder, diameter 0.17 ± 0.04mm accounts for 59% of total vegetalitas carrier.
Described mineral carrier is selected from diatomite, kaolin or attapulgite.Its effect is the flowability that increases bait formulation, is convenient to pack and use.The present invention preferably uses the bead-type substrate of 100-300 order size.
Bait formulation of fungi of the present invention, wherein said vegetable oil can be selected from peanut oil, sunflower oil, Semen Benincasae oil, soybean oil, sesame oil or corn oil.Its effect is to form tempered oil film and can lure cockroach at spore surface.The preferred edible oil that uses in the shelf-life.
Described sugar can be selected from glucose, sucrose or maltose.Work to lure cockroach, powdery.
Cockroach sex hormone and gathering hormone extract solvent and can buy from the market, play attracting action.
The preparation method of embodiment embodiment 15 hundred million spores/gram bait formulation
| Component | Content |
| Metarhizium anisopliae Metarhizium anisopliae IBCCM321.93 | 2％ |
| Vegetalitas carrier 1-corncob | 24％ |
| Vegetalitas carrier 2-rusk powder | 30.9％ |
| Vegetalitas carrier 3-wheat bran | 22％ |
| Diatomite ZX611# | 3％ |
| Semen Benincasae oil | 15％ |
| Groton bug sex hormone and gathering hormone extract solvent | 0.1％ |
| Powdered glucose | 3％ |
Corncob, rusk powder, the wheat bran of aforementioned proportion are put into cracker, are processed into the grain of following size and ratio and the mixture that powder is formed:: bar-shaped grain, length 1.95 ± 0.32mm, diameter 0.66 ± 0.21mm accounts for 27% of total vegetalitas carrier; The sheet grain, length 0.97 ± 0.27mm accounts for 14% of total vegetalitas carrier; Powder, diameter 0.17 ± 0.04mm accounts for 59% of total vegetalitas carrier.
Vegetalitas carrier mixture after will processing then, green muscardine fungus, diatomite, glucose Groton bug sex hormone and assemble that hormone extracts that solvent, Semen Benincasae oil add successively that CC-II type fungal spore separates and mixer in mix, make 500,000,000 spores/gram bait formulation.The gained Bait formulation of fungi is spread fertilizer over the fields at the cockroach place that haunts, usually every square metre of 0.5g preparation.The preparation method of embodiment 2 2.5 hundred million spores/gram bait formulation
| Component | Content |
| Metarhizium anisopliae Metarhizium anisopliae IBCCM321.93 | 1％ |
| Vegetalitas carrier 1-rice husk | 27％ |
| Vegetalitas carrier 2-cracker meal | 30.9％ |
| 100 attapulgites | 30％ |
| Sesame oil | 10％ |
| Groton bug sex hormone and gathering hormone extract solvent | 0.1％ |
| Maltitol powder | 1％ |
Rice husk, the cracker meal of aforementioned proportion are put into cracker, are processed into the grain of following size and ratio and the mixture that powder is formed:: bar-shaped grain, length 1.95 ± 0.32mm, diameter 0.66 ± 0.21mm accounts for 27% of total vegetalitas carrier; The sheet grain, length 0.97 ± 0.27mm accounts for 14% of total vegetalitas carrier; Powder, diameter 0.17 ± 0.04mm accounts for 59% of total vegetalitas carrier.
Vegetalitas carrier mixture after will processing then, green muscardine fungus, attapulgite, maltose, Groton bug sex hormone and assemble that hormone extracts that solvent, sesame oil add successively that CC-II type fungal spore separates and mixer in mix the hundred million spores/gram bait formulation that makes 2: 5.The gained Bait formulation of fungi is spread fertilizer over the fields at the cockroach place that haunts, usually every square metre of 0.5g preparation.The preparation method of embodiment 3 Bait formulation of fungi
| Component | Content |
| Metarhizium anisopliae Metarhizium anisopliae IBCCM321.93 | 20％ |
| Vegetalitas carrier-maize straw | 39.9％％ |
| Peanut oil | 25％ |
| Groton bug sex hormone and gathering hormone extract solvent | 0.1％ |
| Cane sugar powder | 15％ |
The maize straw of aforementioned proportion is put into cracker, is processed into the grain of following size and ratio and the mixture that powder is formed:: bar-shaped grain, length 1.95 ± 0.32mm, diameter 0.66 ± 0.21mm accounts for 27% of total vegetalitas carrier; The sheet grain, length 0.97 ± 0.27mm accounts for 14% of total vegetalitas carrier; Powder, diameter 0.17 ± 0.04mm accounts for 59% of total vegetalitas carrier.
Vegetalitas carrier mixture after will processing then, green muscardine fungus, sucrose, Groton bug sex hormone and assemble that hormone extracts that solvent, peanut oil add successively that CC-II type fungal spore separates and mixer in mix, make 5,000,000,000 spores/gram bait formulation.The gained Bait formulation of fungi is spread fertilizer over the fields at the cockroach place that haunts, usually every square metre of 0.2g preparation.The preparation method of embodiment 4 Bait formulation of fungi
| Component | Content |
| White muscardine fungi | 5％ |
| Vegetalitas carrier 1-rice husk | 23％ |
| Vegetalitas carrier 2-cracker meal | 30.9％ |
| 100 attapulgites | 30％ |
| Sesame oil | 10％ |
| Groton bug sex hormone and gathering hormone extract solvent | 0.1％ |
| Maltitol powder | 1％ |
Rice husk, the cracker meal of aforementioned proportion are put into cracker, be processed into the grain of following size and ratio and the mixture that powder is formed: bar-shaped grain, length 1.95 ± 0.32mm, diameter 0.66 ± 0.21mm accounts for 27% of total vegetalitas carrier; The sheet grain, length 0.97 ± 0.27mm accounts for 14% of total vegetalitas carrier; Powder, diameter 0.17 ± 0.04mm accounts for 59% of total vegetalitas carrier.
Vegetalitas carrier mixture after will processing then, white muscardine fungi, attapulgite, maltose, Groton bug sex hormone and assemble that hormone extracts that solvent, sesame oil add successively that CC-II type fungal spore separates and mixer in mix, make Bait formulation of fungi.The gained Bait formulation of fungi is spread fertilizer over the fields at the cockroach place that haunts, usually every square metre of 0.5g preparation.
Claims (10)
1. a Bait formulation of fungi is characterized in that, described bait formulation is by fungal spore, mineral carrier, vegetable oil, sugar, cockroach sex hormone and the gathering hormone extracts solvent and the vegetalitas carrier is formed.
2. Bait formulation of fungi as claimed in claim 1, it is characterized in that, described components contents is respectively (percentage by weight) fungal spore 1%-20%, mineral carrier 0-30%, vegetable oil 10%-25%, sugared 1%-15%, cockroach sex hormone and assembles hormone and extract solvent 0.1%, and surplus is the vegetalitas carrier.
3. Bait formulation of fungi as claimed in claim 2 is characterized in that described vegetable oil can be selected from peanut oil, sunflower oil, Semen Benincasae oil, sesame oil, soybean oil or corn oil.
4. Bait formulation of fungi as claimed in claim 3 is characterized in that described sugar can be selected from glucose, sucrose or maltose, and is the powder shape.
5. as the arbitrary described Bait formulation of fungi of claim 1 to 4, it is characterized in that described mineral carrier is selected from diatomite, kaolin or attapulgite.
6. Bait formulation of fungi as claimed in claim 5 is characterized in that, described vegetalitas carrier is to be selected from one or more following materials to make: corncob, rice husk, cracker meal, rusk powder, wheat bran and stalk.
7. Bait formulation of fungi as claimed in claim 6 is characterized in that, described vegetalitas carrier is the mixture that grain and powder are formed; Wherein bar-shaped grain, length 1.95 ± 0.32mm, diameter 0.66 ± 0.21mm accounts for 27% of total vegetalitas carrier; The sheet grain, length 0.97 ± 0.27mm accounts for 14% of total vegetalitas carrier; Powder, diameter 0.17 ± 0.04mm accounts for 59% of total vegetalitas carrier.
8. Bait formulation of fungi as claimed in claim 7 is characterized in that, the mineral carrier is the granular of 100-300 order size.
9. Bait formulation of fungi as claimed in claim 8 is characterized in that, described fungal spore is selected from muscardine spore or beauveria bassiana spore and other have pathogenic effects to cockroach fungi; Described fungal spore is pure conidial powder, and purity is greater than 95%, and water content is less than 10%.
10. Bait formulation of fungi as claimed in claim 8 is characterized in that, described fungal spore is a Metarhizium anisopliae IBCCM321.93 spore, and preserving number is CGMCC No.0853.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02156520 CN1183834C (en) | 2002-12-16 | 2002-12-16 | Bait formulation of fungi |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02156520 CN1183834C (en) | 2002-12-16 | 2002-12-16 | Bait formulation of fungi |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1415211A true CN1415211A (en) | 2003-05-07 |
| CN1183834C CN1183834C (en) | 2005-01-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02156520 Expired - Fee Related CN1183834C (en) | 2002-12-16 | 2002-12-16 | Bait formulation of fungi |
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| CN (1) | CN1183834C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108703154A (en) * | 2018-08-07 | 2018-10-26 | 重庆聚立信生物工程有限公司 | Green muscardine fungus kills roach agent and preparation method thereof with boric acid or borax compounding |
| CN113519558A (en) * | 2020-04-16 | 2021-10-22 | 郑州青秋生物防治技术有限公司 | Microbial insecticide formula |
-
2002
- 2002-12-16 CN CN 02156520 patent/CN1183834C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108703154A (en) * | 2018-08-07 | 2018-10-26 | 重庆聚立信生物工程有限公司 | Green muscardine fungus kills roach agent and preparation method thereof with boric acid or borax compounding |
| CN113519558A (en) * | 2020-04-16 | 2021-10-22 | 郑州青秋生物防治技术有限公司 | Microbial insecticide formula |
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| Publication number | Publication date |
|---|---|
| CN1183834C (en) | 2005-01-12 |
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