CN113519558A - Microbial insecticide formula - Google Patents
Microbial insecticide formula Download PDFInfo
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- CN113519558A CN113519558A CN202010301626.1A CN202010301626A CN113519558A CN 113519558 A CN113519558 A CN 113519558A CN 202010301626 A CN202010301626 A CN 202010301626A CN 113519558 A CN113519558 A CN 113519558A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a microbial insecticide formula, which belongs to the technical field of insecticide, and specifically relates to a microbial insecticide formula, wherein raw materials of the microbial insecticide formula comprise effective components, an auxiliary agent and an attractant, wherein the effective components, the auxiliary agent and the attractant respectively account for 0.0-100% of the effective components, 0.0-100% of the auxiliary agent and 0.0-100% of the attractant according to mass proportions; the effective component is destruxins of Metarrhizium anisopliae; the auxiliary agent is whey powder; the attractant is maltose powder, the raw materials of the microbial pesticide formula need to be mixed before use, and the specific steps are as follows: s1: putting the raw materials into mixing equipment in proportion: sequentially putting the effective components, the auxiliary agent and the attractant into mixing equipment; s2: the raw materials are mixed evenly. The pesticide residue problem can be effectively avoided, and the problem of toxin ingestion in the body is fundamentally solved; the microbial pesticide has good effect of killing pests, can kill pests continuously and has long effective period.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a microbial pesticide formula.
Background
Crops are various plants cultivated in agriculture. Including grain crops and economic crops (oil crops, vegetable crops, flowers, grasses and trees). The food is taken by people as days, the relationship between people and food is expressed, and the reasonable meal collocation can bring health to people. The growth of crops can not be separated from scientific technological production technology and novel industrially manufactured mechanical equipment capable of assisting agricultural production.
The agricultural chemical is used for preventing and controlling plant diseases and insect pests and regulating plant growth. It is widely used in agriculture, forestry and animal husbandry production, environment and household sanitation, pest and disease control, and industrial product mildew and moth prevention. The pesticide has various varieties, and can be mainly divided into insecticide, acaricide, rodenticide, nematicide, molluscicide, bactericide, herbicide, plant growth regulator and the like according to the application; according to the raw material source, the pesticide can be divided into mineral source pesticides (inorganic pesticides), biological source pesticides (natural organic matters, microorganisms, antibiotics and the like) and chemical synthetic pesticides; according to chemical structure, mainly comprises organic chlorine, organic phosphorus, organic nitrogen, organic sulfur, carbamate, pyrethroid, amide compounds, urea compounds, ether compounds, phenolic compounds, phenoxy carboxylic acids, amidines, triazoles, heterocycles, benzoic acids, organic metal compounds and the like, which are all organic synthetic pesticides; it can be divided into powder, wettable powder, emulsion, emulsifiable concentrate, emulsifiable paste, cataplasm, colloidal agent, fumigant, aerosol, granule, microgranule, oil agent, etc. according to the preparation formulation.
Cockroach, broadly, refers to an insect belonging to the order "blattaria", belongs to the phylum arthropoda, class insecta, order blattaria, commonly known as a cockroach, and is a common medical insect. Cockroach bodies are flat, dark brown, usually medium in size. Small head and moving. Tentacles are long and filiform, and compound eyes are developed. The wings are flat, the front wing is made of leather, the back wing is made of membranous, the front wing and the back wing are basically equal in size and cover the back of the abdomen; some have no wings. It is not good at flying and can be used for walking quickly. For killing cockroaches, people adopt a mode of pesticide for treatment.
The pesticide residue is easy to occur in crop pest killing, the pesticide residue on crops is easy to absorb pesticide components in the body after people eat the crops, and the long-time accumulation has great harm to the human body.
Disclosure of Invention
The invention aims to provide a microbial pesticide formula to solve the problems that pesticide residues are easy to occur in crop pesticide killing and pesticide residues on crops, and people can easily absorb pesticide components in the body after eating the crops, so that the long-term accumulation of the pesticide components has great harm to human bodies.
In order to achieve the purpose, the invention provides the following technical scheme: a microbial insecticide formula comprises raw materials of an effective component, an auxiliary agent and an attractant, wherein the effective component, the auxiliary agent and the attractant respectively account for 0.0-100% of the effective component, 0.0-100% of the auxiliary agent and 0.0-100% of the attractant according to mass proportion;
the effective component is destruxins of Metarrhizium anisopliae;
the auxiliary agent is whey powder;
the attractant is maltose powder.
Preferably, the effective component, the auxiliary agent and the attractant respectively account for 20% -30% of the effective component, 35% -40% of the auxiliary agent and 35% -40% of the attractant according to mass proportion.
Preferably, the effective component, the auxiliary agent and the attractant respectively account for 25% -35% of the effective component, 32.5% -37.5% of the auxiliary agent and 32.5% -37.5% of the attractant according to mass proportion.
Preferably, the effective component, the auxiliary agent and the attractant respectively account for 40-50 percent of the effective component, 25-30 percent of the auxiliary agent and 25-30 percent of the attractant according to mass proportion.
Preferably, the raw materials of the microbial pesticide formula need to be mixed before use, and the specific steps are as follows:
s1: putting the raw materials into mixing equipment in proportion: cleaning the interior of the mixing equipment, and sequentially placing the effective components, the auxiliary agent and the attractant into the mixing equipment;
s2: uniformly mixing the raw materials: and starting the material mixing equipment, and stirring the raw materials by the material mixing equipment to uniformly mix the raw materials.
Compared with the prior art, the invention has the beneficial effects that:
1) microorganisms are used as effective components for killing insects, so that the problem of pesticide residue can be effectively avoided, and the problem of toxin ingestion in the body is fundamentally solved;
2) the microbial pesticide has good effect of killing pests, can kill pests continuously and has long effective period.
Drawings
FIG. 1 is a flow chart of the mixing of raw materials for the microbial pesticide formulation of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1, the present invention provides a technical solution: a microbial insecticide formula comprises raw materials of an effective component, an auxiliary agent and an attractant, wherein the effective component, the auxiliary agent and the attractant respectively account for 0.0-100% of the effective component, 0.0-100% of the auxiliary agent and 0.0-100% of the attractant according to mass proportion;
the effective component is destruxins of Metarrhizium anisopliae;
the auxiliary agent is whey powder;
the attractant is maltose powder.
The green muscardine fungus is broad-spectrum entomopathogenic fungus, the number of green muscardine fungus host insects reaches more than 200 according to statistics, and the green muscardine fungus host insects can parasitize tortoise shells, weevils, golden moths, lepidoptera pest larvae, hemiptera stinkbug and the like. Can induce insects to generate green muscardine and can form repeated infection in the population. In application, the metarhizium anisopliae is mainly used for preventing and controlling pests, and the metarhizium anisopliae is developed into a fungal pesticide second to beauveria bassiana only in terms of prevention and control scale. The Metarrhizium anisopliae is harmless to human and livestock, safe to natural enemy insects, and free of environmental pollution.
The optimal development temperature is about 24-26 ℃, and the normal development can still be realized at 10-30 ℃; conidia can lose germination capacity quickly at about 60 deg.C. The bacteria can normally develop at pH4.7-10, but the optimum pH is 6.9-7.4. Although Metarrhizium anisopliae can grow on an inorganic culture medium, the culture medium contains an organic nitrogen source which is more suitable for the growth of the Metarrhizium anisopliae than an inorganic ammonia source; metarrhizium anisopliae has no special requirement on a carbon source. Conidia of the green bacteria can be preserved for more than 3 years under dry conditions.
The infection of the host insect by Metarrhizium anisopliae is substantially the same as that by other insects. Conidia of the pseudomonad viridis are easy to attach to internodes of the skin of the host insect, and when the pseudomonad viridis meets appropriate temperature and humidity, the conidia germinate in a row to generate germ tubes and form hyphae. The hyphae secrete enzymes that dissolve chitin, and dissolve the insect body wall. And the hyphae invade the epidermis of the host by means of the pressure generated by the invasion of the host by the bacteria, and further gradually invade inwards to invade the adipose tissues and muscles of the insects. The hyphae multiply in the insect body, which leads to death of the insect. When the host insect is initially infected with Metarrhizium anisopliae, yellowish brown spots are visible on the body wall, and the insect begins to express nervous system disorder due to the action of Metarrhizium anisopliae toxin. Larvae stopped feeding, had a reduced response to the stimulus, and finally died. After death, the corpses are rigidified, hyphae in the corpses begin to extend to the outside, the corpses are quickly coated by a layer of white hyphae, and conidiophores and conidia are formed on the hyphae in the first and second days, and then the corpses become green or dark green.
Preferably, the effective component, the auxiliary agent and the attractant respectively account for 20% -30% of the effective component, 35% -40% of the auxiliary agent and 35% -40% of the attractant according to mass proportion.
Preferably, the effective component, the auxiliary agent and the attractant respectively account for 25% -35% of the effective component, 32.5% -37.5% of the auxiliary agent and 32.5% -37.5% of the attractant according to mass proportion.
Preferably, the effective component, the auxiliary agent and the attractant respectively account for 40-50 percent of the effective component, 25-30 percent of the auxiliary agent and 25-30 percent of the attractant according to mass proportion.
The first embodiment is as follows:
the effective components, the auxiliary agent and the attractant are respectively 20 percent of the effective components, 40 percent of the auxiliary agent and 40 percent of the attractant according to mass proportion;
the raw materials of the microbial pesticide formula need to be mixed before use, and the specific steps are as follows:
s1: putting the raw materials into mixing equipment in proportion: cleaning the interior of the mixing equipment, and sequentially placing the effective components, the auxiliary agent and the attractant into the mixing equipment;
s2: uniformly mixing the raw materials: and starting the material mixing equipment, and stirring the raw materials by the material mixing equipment to uniformly mix the raw materials.
Example two:
the effective component, the auxiliary agent and the attractant are respectively 35 percent of the effective component, 32.5 percent of the auxiliary agent and 32.5 percent of the attractant according to the mass proportion;
the raw materials of the microbial pesticide formula need to be mixed before use, and the specific steps are as follows:
s1: putting the raw materials into mixing equipment in proportion: cleaning the interior of the mixing equipment, and sequentially placing the effective components, the auxiliary agent and the attractant into the mixing equipment;
s2: uniformly mixing the raw materials: and starting the material mixing equipment, and stirring the raw materials by the material mixing equipment to uniformly mix the raw materials.
Example three:
the effective components, the auxiliary agent and the attractant are respectively 40% of the effective components, 30% of the auxiliary agent and 30% of the attractant according to mass proportion;
the raw materials of the microbial pesticide formula need to be mixed before use, and the specific steps are as follows:
s1: putting the raw materials into mixing equipment in proportion: cleaning the interior of the mixing equipment, and sequentially placing the effective components, the auxiliary agent and the attractant into the mixing equipment;
s2: uniformly mixing the raw materials: and starting the material mixing equipment, and stirring the raw materials by the material mixing equipment to uniformly mix the raw materials.
The microbial insecticide powder obtained in the three embodiments is placed in a place where cockroaches usually appear, and the cockroaches can be effectively prevented and treated.
While there have been shown and described the fundamental principles and essential features of the invention and advantages thereof, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof; the present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A microbial insecticide formulation characterized by: the raw materials of the microbial pesticide formula comprise effective components, an auxiliary agent and an attractant, wherein the effective components, the auxiliary agent and the attractant respectively account for 0.0-100 percent of the effective components, 0.0-100 percent of the auxiliary agent and 0.0-100 percent of the attractant according to mass proportion;
the effective component is destruxins of Metarrhizium anisopliae;
the auxiliary agent is whey powder;
the attractant is maltose powder.
2. A microbial insecticide formulation as claimed in claim 1 wherein: the effective component, the auxiliary agent and the attractant respectively account for 20-30 percent of the effective component, 35-40 percent of the auxiliary agent and 35-40 percent of the attractant according to mass proportion.
3. A microbial insecticide formulation as claimed in claim 1 wherein: the effective components, the auxiliary agents and the attractants respectively account for 25-35 percent of the effective components, 32.5-37.5 percent of the auxiliary agents and 32.5-37.5 percent of the attractants according to the mass proportion.
4. A microbial insecticide formulation as claimed in claim 1 wherein: the effective component, the auxiliary agent and the attractant respectively account for 40-50 percent of the effective component, 25-30 percent of the auxiliary agent and 25-30 percent of the attractant according to the mass proportion.
5. A microbial insecticide formulation as claimed in claim 1 wherein: the raw materials of the microbial pesticide formula need to be mixed before use, and the specific steps are as follows:
s1: putting the raw materials into mixing equipment in proportion: cleaning the interior of the mixing equipment, and sequentially placing the effective components, the auxiliary agent and the attractant into the mixing equipment;
s2: uniformly mixing the raw materials: and starting the material mixing equipment, and stirring the raw materials by the material mixing equipment to uniformly mix the raw materials.
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CN202010301626.1A CN113519558A (en) | 2020-04-16 | 2020-04-16 | Microbial insecticide formula |
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CN202010301626.1A CN113519558A (en) | 2020-04-16 | 2020-04-16 | Microbial insecticide formula |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023137785A1 (en) * | 2022-01-21 | 2023-07-27 | 浙江大学 | Application of sativene, recombinant biocontrol fungus, and bombyx batryticatus and application thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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US5804208A (en) * | 1994-11-28 | 1998-09-08 | Bayer Aktiengesellschaft | Granulates containing micro-organisms |
CN1415214A (en) * | 2002-12-16 | 2003-05-07 | 苏红田 | Gluey bait of fungi for killing cockroaches and its preparation method |
CN1415211A (en) * | 2002-12-16 | 2003-05-07 | 张泽华 | Bait formulation of fungi |
CN1448502A (en) * | 2002-12-16 | 2003-10-15 | 张泽华 | Green muscardine and use thereof |
CN106544278A (en) * | 2016-11-21 | 2017-03-29 | 山东师范大学 | A kind of pathogenic fungi for Blatta seu periplaneta Biological control |
-
2020
- 2020-04-16 CN CN202010301626.1A patent/CN113519558A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5804208A (en) * | 1994-11-28 | 1998-09-08 | Bayer Aktiengesellschaft | Granulates containing micro-organisms |
CN1415214A (en) * | 2002-12-16 | 2003-05-07 | 苏红田 | Gluey bait of fungi for killing cockroaches and its preparation method |
CN1415211A (en) * | 2002-12-16 | 2003-05-07 | 张泽华 | Bait formulation of fungi |
CN1448502A (en) * | 2002-12-16 | 2003-10-15 | 张泽华 | Green muscardine and use thereof |
CN106544278A (en) * | 2016-11-21 | 2017-03-29 | 山东师范大学 | A kind of pathogenic fungi for Blatta seu periplaneta Biological control |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023137785A1 (en) * | 2022-01-21 | 2023-07-27 | 浙江大学 | Application of sativene, recombinant biocontrol fungus, and bombyx batryticatus and application thereof |
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Application publication date: 20211022 |