CN111269061A - Celastrus angulatus insecticidal and bactericidal insecticide fertilizer and preparation method thereof - Google Patents

Celastrus angulatus insecticidal and bactericidal insecticide fertilizer and preparation method thereof Download PDF

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Publication number
CN111269061A
CN111269061A CN202010106552.6A CN202010106552A CN111269061A CN 111269061 A CN111269061 A CN 111269061A CN 202010106552 A CN202010106552 A CN 202010106552A CN 111269061 A CN111269061 A CN 111269061A
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celastrus angulatus
insecticidal
preparation
fertilizer
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徐德锋
蔡新红
龚顺泽
胡航
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Changzhou University
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Changzhou University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06Aluminium; Calcium; Magnesium; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/14Boron; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16Heavy metals; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/14Celastraceae [Staff-tree or Bittersweet family], e.g. spindle tree, bittersweet or thunder god vine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/48Zingiberaceae [Ginger family], e.g. ginger or galangal
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium
    • C05D1/02Manufacture from potassium chloride or sulfate or double or mixed salts thereof
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a celastrus angulatus insecticidal sterilization medical fertilizer and a preparation method thereof. The insecticidal sterilization medical fertilizer for celastrus angulatus comprises the following components: 0.2-5 parts of celastrus angulatus, 10-30 parts of celastrus angulatus extraction residues, 0.2-3 parts of turmeric, 5-10 parts of turmeric extraction residues, 20-30 parts of soybean meal, 20-30 parts of crop straws, 5-10 parts of sawdust, 15-20 parts of humic acid, 3-5 parts of biological bacteria preparation, 1-2 parts of magnesium sulfate, 1-2 parts of zinc sulfate, 1-2 parts of boric acid, 1-2 parts of salicylic acid, 1-2 parts of potassium chloride, 2-4 parts of adhesive starch, 1-2 parts of dextrin and 15-30 parts of water. The celastrus angulatus insecticidal and sterilization medical fertilizer prepared by the invention has good insecticidal and sterilization effects and can improve the yield of crops.

Description

Celastrus angulatus insecticidal and bactericidal insecticide fertilizer and preparation method thereof
Technical Field
The invention relates to the technical field of production of medicinal fertilizers, and particularly relates to a celastrus angulatus insecticidal and sterilization medicinal fertilizer and a preparation method thereof.
Background
Crop diseases and insect pests are one of the main agricultural disasters, and have the characteristics of multiple types, large influence and frequent outbreak of disasters, and the occurrence range and the severity of the crop diseases and insect pests can cause great loss to agricultural production. At present, crop diseases and insect pests are controlled, crop yield is improved, mainly chemical pesticides are used for prevention and control, and a large amount of chemical pesticides not only pollute the environment, but also poison people and livestock.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the use of a large amount of chemical pesticide not only pollutes the environment, but also causes toxicity to people and livestock.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the insecticidal and bactericidal celastrus angulatus insecticide fertilizer comprises the following components in parts by weight: 0.2-5 parts of celastrus angulatus, 10-30 parts of celastrus angulatus extraction residues, 0.2-3 parts of turmeric, 5-10 parts of turmeric extraction residues, 20-30 parts of soybean meal, 20-30 parts of crop straws, 5-10 parts of sawdust, 15-20 parts of humic acid, 3-5 parts of biological bacteria preparation, 1-2 parts of magnesium sulfate, 1-2 parts of zinc sulfate, 1-2 parts of boric acid, 1-2 parts of salicylic acid, 1-2 parts of potassium chloride, 2-4 parts of adhesive starch, 1-2 parts of dextrin and 15-30 parts of water.
Furthermore, the crop straws comprise one or more of rice straws, wheat straws and corn straws.
Further, the adhesive starch comprises one or more of mung bean starch, sweet potato starch, potato starch and corn starch.
Further, the biological bacterial preparation comprises one or more of candida tropicalis, bacillus subtilis, bacillus cereus, bacillus megaterium and bacillus mucilaginosus.
Furthermore, the biological bacteria preparation contains 2-3 hundred million of live bacterins.
Further, the dextrin is corn dextrin.
Further, the preparation method of the celastrus angulatus insecticidal and sterilization medical fertilizer comprises the following steps:
(1) pulverizing Celastrus angulatus, Curcuma rhizome, soybean meal and crop straw, adding water for humidification, adding Celastrus angulatus extraction residue, Curcuma rhizome extraction residue, sawdust, humic acid and biological bacteria preparation, mixing well, stacking for fermentation, turning over, and performing high temperature fermentation to obtain completely fermented organic fertilizer for later use.
(2) Mixing the completely fermented organic fertilizer with magnesium sulfate, zinc sulfate, boric acid, salicylic acid and potassium chloride, adding adhesive starch and dextrin, uniformly mixing, extruding and granulating to obtain the celastrus angulatus insecticidal sterilization medicinal fertilizer.
Further, the number of days for the stacking fermentation in the step (1) is 5 to 10 days.
Further, the fermentation temperature of the high-temperature fermentation in the step (1) is 70-85 ℃.
Further, the fermentation time of the high-temperature fermentation in the step (1) is 30-45 days.
The invention has the beneficial effects that:
(1) the celastrus angulatus insecticidal and sterilization medical fertilizer is prepared by taking celastrus angulatus and turmeric as raw materials, wherein the root bark of the celastrus angulatus can prevent and treat plant diseases and insect pests such as Chinese cabbage and radish and is harmless to people and livestock, the celastrus angulatus contains β -dihydroagarofuran polyol ester compounds, the β -dihydroagarofuran polyol ester compounds are effective insecticidal components and have anesthesia, antifeedant and poisoning effects on pests, the turmeric is a zingiberaceae plant, the turmeric contains active ingredients such as turmerone, zingiberene, phellandrene, dehydroturmerone and curcumin, has a good antibacterial effect on staphylococcus aureus, and can be used as a green environment-friendly bactericide for sterilization and prevention of crops;
(2) the celastrus angulatus insecticidal and sterilization pesticide fertilizer prepared by the invention has a very wide pest control spectrum, has control effects on various pests, can control cabbage caterpillars, turnip, leaf bee larvae, rice skipper, looper larvae, cucumber and the like on vegetables such as cabbage, cauliflower, Chinese cabbage and the like by mixing the celastrus angulatus and the turmeric to prepare the pesticide fertilizer, and also has good control effects on pests of crops such as rice, wheat, cotton, tea and the like. The celastrus angulatus insecticidal and sterilization pesticide fertilizer prepared by the invention is easy to photolyze, hydrolyze and degrade soil in natural environment, and cannot pollute underground water. The celastrus angulatus insecticidal and sterilization medical fertilizer prepared by the invention reduces the usage amount of chemical pesticides, adopts natural pure plant traditional Chinese medicines celastrus angulatus and turmeric, can improve the soil of crops, protects the primary environment, and can improve the yield of the crops.
Detailed Description
A preparation method of celastrus angulatus insecticidal and sterilization medical fertilizer comprises the following steps:
respectively weighing 0.2-5 parts of celastrus angulatus, 10-30 parts of celastrus angulatus extraction residues, 0.2-3 parts of turmeric, 5-10 parts of turmeric extraction residues, 20-30 parts of soybean meal, 20-30 parts of crop straws, 5-10 parts of wood chips, 15-20 parts of humic acid, 3-5 parts of biological bacteria preparation, 1-2 parts of magnesium sulfate, 1-2 parts of zinc sulfate, 1-2 parts of boric acid, 1-2 parts of salicylic acid, 1-2 parts of potassium chloride, 2-4 parts of adhesive starch, 1-2 parts of dextrin and 15-30 parts of water according to parts by weight. Pulverizing Celastrus angulatus, Curcuma rhizome, soybean meal and crop straw, adding water for humidification, adding Celastrus angulatus extraction residue, Curcuma rhizome extraction residue, sawdust, humic acid and biological bacteria preparation, mixing uniformly, stacking for fermentation for 5-10 days, turning over, and performing high temperature fermentation at 70-85 deg.C for 30-45 days to obtain completely fermented organic fertilizer. Mixing the completely fermented organic fertilizer with magnesium sulfate, zinc sulfate, boric acid, salicylic acid and potassium chloride, adding adhesive starch and dextrin, uniformly mixing, extruding and granulating to obtain the celastrus angulatus insecticidal sterilization medicinal fertilizer.
The crop straw comprises one or more of rice straw, wheat straw and corn straw.
The adhesive starch comprises one or more of mung bean starch, sweet potato starch, potato starch and corn starch.
The biological bacteria preparation contains 2-3 hundred million of live bacterins, and comprises one or more of candida tropicalis, bacillus subtilis, bacillus cereus, bacillus megaterium and bacillus mucilaginosus.
The dextrin is corn dextrin.
Example 1
The preparation method of the celastrus angulatus insecticidal and sterilization medicinal fertilizer comprises the following steps:
respectively weighing 5 parts of celastrus angulatus, 30 parts of celastrus angulatus extraction residues, 3 parts of curcuma longa, 10 parts of curcuma longa extraction residues, 20 parts of soybean meal, 20 parts of rice straws, 5 parts of sawdust, 15 parts of humic acid, 5 parts of candida tropicalis preparation, 2 parts of magnesium sulfate, 1 part of zinc sulfate, 1 part of boric acid, 1 part of salicylic acid, 1 part of potassium chloride, 4 parts of mung bean starch, 1 part of dextrin and 15 parts of water according to parts by weight. Pulverizing celastrus angulatus, turmeric, soybean meal and rice straw, adding water for humidification, adding celastrus angulatus extraction residue, turmeric extraction residue, wood chips, humic acid and candida tropicalis preparation, uniformly mixing, stacking for fermentation, turning over for 10 days, and performing high-temperature fermentation at the fermentation temperature of 85 ℃ for 45 days to obtain the completely fermented organic fertilizer for later use. Mixing the completely fermented organic fertilizer with magnesium sulfate, zinc sulfate, boric acid, salicylic acid and potassium chloride, adding mung bean starch and dextrin, uniformly mixing, extruding and granulating to obtain the celastrus angulatus insecticidal sterilization medicinal fertilizer.
Example 2
The preparation method of the celastrus angulatus insecticidal and sterilization medicinal fertilizer comprises the following steps:
respectively weighing 0.2 part of celastrus angulatus, 30 parts of celastrus angulatus extraction residues, 0.2 part of curcuma longa, 10 parts of curcuma longa extraction residues, 30 parts of soybean meal, 20 parts of wheat straws, 5 parts of sawdust, 20 parts of humic acid, 3 parts of bacillus subtilis preparation, 1 part of magnesium sulfate, 2 parts of zinc sulfate, 2 parts of boric acid, 2 parts of salicylic acid, 2 parts of potassium chloride, 2 parts of sweet potato starch, 2 parts of dextrin and 30 parts of water according to parts by weight. Pulverizing celastrus angulatus, turmeric, soybean meal and wheat straw, adding water for humidification, adding celastrus angulatus extraction residues, turmeric extraction residues, wood chips, humic acid and bacillus subtilis preparation, uniformly mixing, stacking for fermentation, turning over for 5 days, and performing high-temperature fermentation at the fermentation temperature of 70 ℃ for 30 days to obtain the completely fermented organic fertilizer for later use. Mixing the completely fermented organic fertilizer with magnesium sulfate, zinc sulfate, boric acid, salicylic acid and potassium chloride, adding sweet potato starch and dextrin, uniformly mixing, extruding and granulating to obtain the celastrus angulatus insecticidal sterilization medicinal fertilizer.
Example 3
The preparation method of the celastrus angulatus insecticidal and sterilization medicinal fertilizer comprises the following steps:
respectively weighing 3 parts of celastrus angulatus, 20 parts of celastrus angulatus extraction residues, 2 parts of turmeric, 8 parts of turmeric extraction residues, 25 parts of soybean meal, 25 parts of corn straws, 8 parts of sawdust, 18 parts of humic acid, 4 parts of bacillus cereus preparation, 1.5 parts of magnesium sulfate, 1.5 parts of zinc sulfate, 1.5 parts of boric acid, 1.5 parts of salicylic acid, 1.5 parts of potassium chloride, 2 parts of potato starch, 1 part of dextrin and 20 parts of water according to parts by weight. Pulverizing Celastrus angulatus, Curcuma longa, soybean meal and corn stalks, adding water for humidification, adding Celastrus angulatus extraction residues, Curcuma longa extraction residues, wood chips, humic acid and bacillus cereus preparation, mixing uniformly, stacking for fermentation, turning over once in 8 days, and performing high-temperature fermentation at the fermentation temperature of 80 ℃ for 40 days to obtain the completely fermented organic fertilizer for later use. Mixing the completely fermented organic fertilizer with magnesium sulfate, zinc sulfate, boric acid, salicylic acid and potassium chloride, adding potato starch and dextrin, uniformly mixing, extruding and granulating to obtain the celastrus angulatus insecticidal sterilization medicinal fertilizer.
Example 4
The preparation method of the celastrus angulatus insecticidal and sterilization medicinal fertilizer comprises the following steps:
respectively weighing 4 parts of celastrus angulatus, 25 parts of celastrus angulatus extraction residues, 43 parts of turmeric, 10 parts of turmeric extraction residues, 25 parts of soybean meal, 20 parts of corn straws, 10 parts of sawdust, 20 parts of humic acid, 5 parts of bacillus megaterium preparation, 1.5 parts of magnesium sulfate, 2 parts of zinc sulfate, 1 part of boric acid, 1 part of salicylic acid, 2 parts of potassium chloride, 3 parts of corn starch, 1 part of dextrin and 25 parts of water according to parts by weight. Pulverizing celastrus angulatus, turmeric, soybean meal and corn stalks, adding water for humidification, adding celastrus angulatus extraction residues, turmeric extraction residues, wood chips, humic acid and a bacillus megaterium preparation, uniformly mixing, stacking for fermentation, turning over for 10 days, and performing high-temperature fermentation at the fermentation temperature of 75 ℃ for 40 days to obtain a completely fermented organic fertilizer for later use. Mixing the completely fermented organic fertilizer with magnesium sulfate, zinc sulfate, boric acid, salicylic acid and potassium chloride, adding corn starch and dextrin, uniformly mixing, extruding and granulating to obtain the celastrus angulatus insecticidal sterilization medicinal fertilizer.
Experimental example 5
(1) Reagent preparation
Reagent A: 0.2% celastrus angulatus insecticide solution;
and (3) reagent B: the celastrus angulatus insecticidal and bactericidal insecticide fertilizer prepared in example 1;
and (3) reagent C: the celastrus angulatus insecticidal and bactericidal insecticide fertilizer prepared in example 2;
and (3) reagent D: the celastrus angulatus insecticidal and bactericidal insecticide fertilizer prepared in example 1;
and (3) reagent E: the celastrus angulatus insecticidal and bactericidal insecticide fertilizer prepared in example 2;
and (3) reagent F: and (3) water.
(2) Virulence determination assay
Preparing a test material:
selecting the chilo suppressalis which is bred indoors, has consistent individual size, is healthy and active and is 3-5 days old.
Shearing a double-sided adhesive tape into 2cm long, sticking the double-sided adhesive tape to one end of a glass slide, selecting healthy chilo suppressalis under a microscope, picking up a plurality of chilo suppressalis by using a capillary pen, sticking the backs of the chilo suppressalis to the double-sided adhesive (avoiding sticking chilo suppressalis feet and whiskers), placing 30 pieces of chilo suppressalis into a container filled with wet sponge, covering the container with a cover, placing the container in a room-temperature environment, performing microscopic examination after 2 hours, removing dead and injured individuals, and supplementing 30 pieces of chilo suppressalis.
The test method comprises the following steps:
and (3) soaking the slide with the chilo suppressalis in a reagent A, a reagent B, a reagent C, a reagent D and a reagent E which are equal in amount respectively, slightly oscillating, taking out, absorbing redundant liquid medicine by using absorbent paper, placing in a white porcelain plate padded with wet sponges, covering by using a plastic film with good light transmittance, and repeating the test for 4 times in each group. The test insects are raised and observed under the conditions of normal temperature, humidity of 60-80% and photoperiod of L: D ═ 16:8) h, and the total number of insects and death number are recorded after 72 h.
Criteria for death: the body of the insect is touched with the brush pen and cannot move.
The virulence determinations obtained from the above experiments are shown in table 1 below:
table 1: virulence determination meter
Figure BDA0002388658020000061
Figure BDA0002388658020000071
(3) Sterilization Activity test
The test method comprises the following steps: referring to NY/T1156.2-2006 in pesticide indoor bioassay test standard bactericide, a pathogenic fungus hypha growth inhibition test adopts a plate method to respectively perform indoor activity measurement of fusarium oxysporum cucumber specialization type ((Fusarium oxysporum (Schl.) F. spcucumerinum Owen)) on a reagent A, a reagent B, a reagent C, a reagent D and a reagent E, and the hypha growth inhibition rate formula is as follows after inoculation for 7 days:
Figure BDA0002388658020000072
the measurement results are shown in table 2:
table 2: sterilization activity test table
Classes of reagents Hypha biological inhibition ratio (%)
A 58.0
B 99.0
C 100.0
D 98.0
E 97.0
(4) Test for increasing yield of rice
The test method comprises the following steps: the reagent A, the reagent B, the reagent C, the reagent D, the reagent E and the reagent F are respectively acted on 6 rice fields with consistent growth vigor in the rice growth period, the application amount is 1.5 kg/mu, the yield of the rice in the season is measured, and the rice yield increase measurement result is shown in table 3:
table 3: rice yield increasing measuring meter
Classes of reagents Yield (kg/mu) Yield increase (%)
A 564.8 11.1
B 588.9 15.8
C 618.4 21.6
D 612.3 20.4
E 598.6 17.7
F 508.5 -
Experimental example 5 shows that the celastrus angulatus insecticidal and sterilization medical fertilizer prepared by the invention in examples 1-4 has good insecticidal and sterilization effects and can improve the yield of crops.
In light of the foregoing description of preferred embodiments in accordance with the invention, it is to be understood that numerous changes and modifications may be made by those skilled in the art without departing from the scope of the invention. The technical scope of the present invention is not limited to the contents of the specification, and must be determined according to the scope of the claims.

Claims (10)

1. The insecticidal and bactericidal celastrus angulatus insecticide fertilizer is characterized by comprising the following components in parts by weight: 0.2-5 parts of celastrus angulatus, 10-30 parts of celastrus angulatus extraction residues, 0.2-3 parts of turmeric, 5-10 parts of turmeric extraction residues, 20-30 parts of soybean meal, 20-30 parts of crop straws, 5-10 parts of sawdust, 15-20 parts of humic acid, 3-5 parts of biological bacteria preparation, 1-2 parts of magnesium sulfate, 1-2 parts of zinc sulfate, 1-2 parts of boric acid, 1-2 parts of salicylic acid, 1-2 parts of potassium chloride, 2-4 parts of adhesive starch, 1-2 parts of dextrin and 15-30 parts of water.
2. The celastrus angulatus insecticidal and bactericidal insecticide fertilizer according to claim 1, wherein the crop straws comprise one or more of rice straws, wheat straws and corn straws.
3. The celastrus angulatus insecticidal and bactericidal insecticide fertilizer as claimed in claim 1, wherein the adhesive starch comprises one or more of mung bean starch, sweet potato starch, potato starch and corn starch.
4. The celastrus angulatus insecticidal and bactericidal insecticide fertilizer according to claim 1, wherein the biological bacterial preparation comprises one or more of candida tropicalis, bacillus subtilis, bacillus cereus, bacillus megaterium and bacillus mucilaginosus.
5. The celastrus angulatus insecticidal and bactericidal insecticide-fertilizer according to claim 1, wherein 2-3 hundred million of active bacterins are contained in the biological bacterial preparation.
6. The celastrus angulatus insecticidal and bactericidal insecticide fertilizer according to claim 1, wherein the dextrin is corn dextrin.
7. The preparation method of the celastrus angulatus insecticidal and sterilization medicinal fertilizer according to claim 1, is characterized by comprising the following steps:
(1) pulverizing Celastrus angulatus, Curcuma rhizome, soybean meal and crop straw, adding water for humidification, adding Celastrus angulatus extraction residue, Curcuma rhizome extraction residue, sawdust, humic acid and biological bacteria preparation, mixing well, stacking for fermentation, turning over, and performing high temperature fermentation to obtain completely fermented organic fertilizer for later use.
(2) Mixing the completely fermented organic fertilizer with magnesium sulfate, zinc sulfate, boric acid, salicylic acid and potassium chloride, adding adhesive starch and dextrin, uniformly mixing, extruding and granulating to obtain the celastrus angulatus insecticidal sterilization medicinal fertilizer.
8. The preparation method of celastrus angulatus insecticidal and bactericidal insecticide-fertilizer according to claim 7, wherein the number of days of stacking fermentation in step (1) is 5-10 days.
9. The preparation method of celastrus angulatus insecticidal and sterilization medical fertilizer according to claim 7, wherein the fermentation temperature of the high temperature fermentation in the step (1) is 70-85 ℃.
10. The preparation method of the celastrus angulatus insecticidal and sterilization medical fertilizer according to claim 7, wherein the fermentation time of the high-temperature fermentation in the step (1) is 30-45 days.
CN202010106552.6A 2020-02-21 2020-02-21 Celastrus angulatus insecticidal and bactericidal insecticide fertilizer and preparation method thereof Pending CN111269061A (en)

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