CN116711738B - Biocontrol agent and application thereof in spodoptera frugiperda control - Google Patents

Biocontrol agent and application thereof in spodoptera frugiperda control Download PDF

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Publication number
CN116711738B
CN116711738B CN202310702472.0A CN202310702472A CN116711738B CN 116711738 B CN116711738 B CN 116711738B CN 202310702472 A CN202310702472 A CN 202310702472A CN 116711738 B CN116711738 B CN 116711738B
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spodoptera frugiperda
acetate
entomopathogenic nematodes
biocontrol
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CN116711738A (en
Inventor
梁玉勇
漆学伟
洪霖
程森弟
程正新
王旭明
贾志新
万鹏
钟秋瓒
谢金招
孙强
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Institute Of Plant Protection Jiangxi Academy Of Agricultural Sciences
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Institute Of Plant Protection Jiangxi Academy Of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • A01N63/12—Nematodes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
    • A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P19/00—Pest attractants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/02—Acaricides

Abstract

The invention discloses a biocontrol preparation and application thereof in spodoptera frugiperda control, wherein the biocontrol preparation comprises entomopathogenic nematodes, sucrose, leaf alcohol acetate and nutritional aids; the entomopathogenic nematodes are the nematodes Steinernema carpocapsae; the content of the leaf alcohol acetate in the biocontrol agent is 1-1.3%; the nutrition auxiliary agent comprises the following components in parts by mass: 3 to 6 parts of peptone, 4 to 5.5 parts of beef extract, 3 to 4.5 parts of agar powder, 3 to 4.5 parts of wheat flour, 3.2 to 4.7 parts of corn oil, 10 to 12 parts of corn leaf alcohol extract and 5.2 to 5.7 parts of yolk powder; the invention uses entomopathogenic nematodes to control spodoptera frugiperda, mainly traps and kills spodoptera frugiperda larvae and female insects in mating period under the dual action of sucrose and phyllostanol acetate, so that mass reproduction of spodoptera frugiperda is fundamentally avoided, and the spodoptera frugiperda control effect is good.

Description

Biocontrol agent and application thereof in spodoptera frugiperda control
Technical Field
The invention belongs to the technical field of crop pest control, and particularly relates to a biocontrol agent and application thereof in spodoptera frugiperda control.
Background
Spodoptera frugiperda (subject Spodoptera frugiperda) is an insect of the genus spodoptera of the family spodopteraceae. Spodoptera frugiperda has very strong diffusion capability, and the spodoptera frugiperda is first transmitted into Asian areas in 2016 from America invasion Africa, 2018, 8 months, and the combined grain and agricultural organization lists the pest as ten plant pests in the world. In 12 months 2018, spodoptera frugiperda invades China for the first time, and is distributed in 26 provinces and cities (areas) of a grain main production area of China in less than one year, so that the spodoptera frugiperda has been developed into important pests in agriculture of China, and serious threat is caused to grain safety of China. Spodoptera frugiperda mainly takes larvae as a pest, and the host range is up to 353 plants in 76 families, especially can cause serious pest on crops such as corn, rice, soybean, sorghum and the like, and is also an important pest on economic crops such as sugarcane, tobacco and the like (Montezano, 2018). At present, the prevention and treatment of spodoptera frugiperda are mainly chemical prevention and treatment, such as single dose: emamectin benzoate, indoxacarb, tetrachlorantraniliprole, chlorantraniliprole, lambda-cyhalothrin, fenpropathrin, acephate, lufenuron, chlorfenapyr, cabbage looper nuclear polyhedrosis virus, bacillus thuringiensis, metarhizium anisopliae, beauveria bassiana, eimeria brevifolia and spodoptera frugiperda sex attractant. The compound preparation comprises the following steps: emamectin benzoate indoxacarb, emamectin benzoate hexaflumuron, emamectin benzoate lambda-cyhalothrin, emamectin benzoate lufenuron, emamectin benzoate tebufenozide, chlorantraniliprole lambda-cyhalothrin, et cetera.
Although the chemical control method is fast and efficient, the chemical control method is not beneficial to the environment and the grain safety, so that an environment-friendly and remarkable spodoptera frugiperda control method is needed.
Disclosure of Invention
In view of the above, the invention aims to provide a biocontrol agent and application thereof in spodoptera frugiperda control.
In order to solve the problems, the invention adopts the following technical scheme:
the technical scheme of the invention is to provide a biocontrol agent which comprises entomopathogenic nematodes, sucrose, phyllostanol acetate and nutritional aids; the entomopathogenic nematodes are the nematodes Steinernema carpocapsae; the content of the leaf alcohol acetate in the biocontrol agent is 1-1.3%.
As a further preferred aspect, the nutritional aid comprises, in parts by mass: 3 to 6 parts of peptone, 4 to 5.5 parts of beef extract, 3 to 4.5 parts of agar powder, 3 to 4.5 parts of wheat flour, 3.2 to 4.7 parts of corn oil, 10 to 12 parts of corn leaf alcohol extract and 5.2 to 5.7 parts of yolk powder.
As a further preferred aspect, the content of the entomopathogenic nematodes in the biocontrol agent is 5700000 IJs-5900000 IJs.
As a further preference, the mass ratio of sucrose to phyllosol acetate is 5-10:1.
As a further preferred aspect, the nutritional aid comprises, in parts by mass: 5 parts of peptone, 5.5 parts of beef extract, 3 parts of agar powder, 4 parts of wheat flour, 4.5 parts of corn oil, 10 parts of corn leaf alcohol extract and 5.5 parts of yolk powder.
As a further preference, the mass ratio of sucrose to leaf alcohol acetate is 9:1.
The technical scheme of the second aspect of the invention is to provide the application of the biocontrol agent in the aspect of controlling crop spodoptera frugiperda.
Further preferably, the crop is corn.
Further preferably, the control time is 6-8 leaf ages of corn.
Further preferably, the biocontrol agent is applied in an amount to ensure that the entomopathogenic nematodes are present in an amount of 1.5X10 9 IJs/mu-1.8X10 9 IJs/mu, and is diluted by 10 times with water before use.
Entomopathogenic nematodes (Entomopathogenic nematode, EPN) are characterized by both "natural enemy insects" and "pathogenic microorganisms". Is safe and friendly to human, livestock and environment, is easy to cultivate in large quantity, can be recycled in the environment, and has durability to crop pest control. The entomopathogenic nematodes enter the body of the pest through the oral cavity, the body wall or the anus of the pest, and after entering the body of the pest, the entomopathogenic nematodes release symbiotic bacteria, and the symbiotic bacteria secrete toxins to destroy the physiological defense function of the insect, so that the insect can die within 24-48 hours after septicemia. Meanwhile, the entomopathogenic nematodes and symbiotic bacteria consume nutrient substances in the insect bodies together to carry out self-reproduction until the nutrient substances of the insect are consumed, and the larvae in the infection stage climb out of the insect bodies to continuously search for host insects, so that sustainable control of the entomopathogenic nematodes is formed.
The phyllostanol acetate can cause olfactory reaction of mating female moths, so that female moths in mating period are attracted to the position of the biocontrol agent, and the female moths are led into the body while feeding on nutrient substances in the biocontrol agent, so that the entomopathogenic nematodes play a role in preventing and controlling.
Both the trametes prandial and the side trametes prandial sensors are present in taste receptor neurons that are sensitive to sucrose, so sucrose can be used as food intake kinetin for spodoptera prandial, especially its larvae.
The invention has the following beneficial effects:
the invention uses entomopathogenic nematodes to control spodoptera frugiperda, mainly traps and kills spodoptera frugiperda larvae and female insects in mating period under the dual action of sucrose and phyllostanol acetate, and fundamentally avoids mass reproduction of spodoptera frugiperda. The nutritional auxiliary agent comprises starch, vegetable oil and protein, wherein the vegetable oil is corn oil, and the corn leaf alcohol extract is added, so that the nutritional substances have the taste of corn leaves, and the spodoptera frugiperda can eat the nutritional substances as much as possible before death, so that corn is prevented from being eaten.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application, illustrate and explain the application and are not to be construed as limiting the application. In the drawings:
FIG. 1 is a graph of the control of spodoptera frugiperda according to various embodiments of the present invention.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
The "room temperature" as used herein is calculated as 25.+ -. 2 ℃ unless otherwise indicated.
The invention discloses a biocontrol agent, which comprises entomopathogenic nematodes, sucrose, leaf alcohol acetate and nutrition auxiliary agents; the entomopathogenic nematodes are the nematodes Steinernema carpocapsae; the content of the leaf alcohol acetate in the biocontrol agent is 1-1.3%.
The nutrition auxiliary agent has the function of enabling the spodoptera frugiperda to be ingested into the body simultaneously in the feeding process, and can be used for the insect pathogen nematodes to ingest nutrition. Thus, on the one hand, good mouthfeel and, on the other hand, sufficient nutrients are required. The nutrition auxiliary agent comprises the following components in parts by mass: 3 to 6 parts of peptone, 4 to 5.5 parts of beef extract, 3 to 4.5 parts of agar powder, 3 to 4.5 parts of wheat flour, 3.2 to 4.7 parts of corn oil, 10 to 12 parts of corn leaf alcohol extract and 5.2 to 5.7 parts of yolk powder. The raw materials and the proportion are screened out through experiments, and the requirements can be met within the range. As a representative but non-limiting alternative, the nutritional aids described in the embodiments of the present invention include, in parts by mass: 5 parts of peptone, 5.5 parts of beef extract, 3 parts of agar powder, 4 parts of wheat flour, 4.5 parts of corn oil, 10 parts of corn leaf alcohol extract and 5.5 parts of yolk powder.
In some preferred embodiments, the content of the entomopathogenic nematodes in the biocontrol agent is 5700000IJs to 5900000IJs.
In some preferred embodiments, the sucrose to phyllosol acetate mass ratio is between 5 and 10:1. More preferred embodiments are 5:1, 7:1, 9:1 and 10:1.
In a further preferred embodiment the mass ratio of sucrose to leaf alcohol acetate is 9:1.
The invention also provides application of the biocontrol agent in preventing and controlling crop spodoptera frugiperda. The crop may be corn and rice.
In a further preferred embodiment, the crop is corn.
In a further preferred embodiment, the biological agent is used for controlling spodoptera frugiperda for a period of 6-8 leaf ages in corn.
In a further preferred embodiment, the biocontrol agent is applied in an amount to ensure that the entomopathogenic nematodes are used in an amount of 1.5X10 9 IJs/mu-1.8X10 9 IJs/mu, and is diluted by 10 times with water before use.
The corn leaf alcohol extract in the following examples is obtained by crushing fresh green corn leaves, reflux-extracting with ethanol, and concentrating, and the method is a conventional method and will not be described here.
The starting materials used in the examples below were all commercially available. Wherein, the leaf alcohol acetate is purchased from Aladin; entomopathogenic nematodes, s.elegans Steinernema carpocapsae, were purchased from the insect institute of cantonese and stored in a refrigerator prior to use. The rest raw materials are obtained through a common online shopping way.
Example 1
A biocontrol agent comprising entomopathogenic nematodes, sucrose, leaf alcohol acetate and a nutritional aid; the entomopathogenic nematodes are the nematodes Steinernema carpocapsae; the content of the leaf alcohol acetate in the biocontrol agent is 1.2%.
The nutrition auxiliary agent comprises the following components in parts by mass: 5 parts of peptone, 5.5 parts of beef extract, 3 parts of agar powder, 4 parts of wheat flour, 4.5 parts of corn oil, 10 parts of corn leaf alcohol extract and 5.5 parts of yolk powder.
The content of the entomopathogenic nematodes in the biocontrol agent is 5700000 IJs-5900000 IJs.
The mass ratio of sucrose to phyllitol acetate is 9:1.
The preparation method comprises simply mixing the above materials. In order to prevent segregation, the biocontrol agent needs to be prepared at present.
Examples 2 to 4
Examples 2 to 4 were set with the mass ratio of sucrose to leaf alcohol acetate as a single variable, wherein the proportions of examples 2 to 4 were 5:1, 7:1 and 10:1 in this order, and the remainder were the same as in example 1.
Examples 5 to 7
The only difference from example 1 is that the content of phyllanthus acetate in the biocontrol formulation is 1% (example 5), 1.1% (example 6) and 1.3% (example 7).
Example 8
A biocontrol agent comprising entomopathogenic nematodes, sucrose, leaf alcohol acetate and a nutritional aid; the entomopathogenic nematodes are the nematodes Steinernema carpocapsae; the content of the leaf alcohol acetate in the biocontrol agent is 1.2%.
The nutrition auxiliary agent comprises the following components in parts by mass: 3 parts of peptone, 5 parts of beef extract, 4 parts of agar powder, 4.5 parts of wheat flour, 3.2 parts of corn oil, 12 parts of corn leaf alcohol extract and 5.2 parts of yolk powder.
The content of the entomopathogenic nematodes in the biocontrol agent is 5700000 IJs-5900000 IJs.
The mass ratio of sucrose to phyllitol acetate is 9:1.
The preparation method comprises simply mixing the above materials.
Comparative example 1
The only difference from example 1 is that no sucrose was added.
Comparative example 2
The only difference from example 1 is that no leaf alcohol acetate was added.
Comparative example 3
The only difference from example 1 is that sucrose and leaf alcohol acetate are not added.
Comparative example 4
The only difference from example 1 is that the corn oil was replaced with soybean oil in equal amounts, while omitting the use of corn leaf alcohol extract.
Application example
1) Control time: the corn is selected in 6-8 leaf ages, and the application time is 5 pm.
2) The application method comprises the following steps: the biocontrol agent is sprayed into the corn cob.
3) Application amount: to ensure that the amount of the entomopathogenic nematodes is 1.8X10 9 IJs/mu, and is diluted by 10 times with water before use.
4) Investigation time and number: investigating the number of insect population before administration, and investigating the number of residual living insects 1, 3 and 7 days after administration; leaf-protecting effect was investigated 7 days after the drug.
5) Investigation method
Larva prevention effect: each treatment cell was investigated using "W" sampling at 5 points, 20 plants each, and the number of live larvae counted.
And (5) protecting She Xiaoguo: the investigation used a 5-point sampling method, and 20 lines of maize were investigated per test cell. Corn leaf damage was investigated and ranked on day 7 post-dose.
Level 0: the new leaves are not damaged
Stage 1: the new leaves have smaller harmful spots
3 stages: the harmful spots are connected into a sheet and a transparent film appears
5 stages: the damage has caused new leaves to be notched
7 stages: the damage causes bigger and more notches on new leaves
Stage 9: the damage causes new leaf fracture
6) The drug effect calculation method comprises the following steps:
wherein: pt (Pt) 0 : a medicament treatment area before medicament treatment; CK (CK) 0 : blank control area pre-drug.
Pt 1 : after the medicine treatment area is used for medicine treatment; CK (CK) 1 : after the blank control area.
7) Results and analysis
TABLE 1 insecticide control of Spodoptera frugiperda protector She Xiaoguo
Medicament treatment Index of blade damage Protector She Xiaoguo (%)
Treatment group 1 6.21 90.34
Treatment group 2 7.15 88.88
Treatment group 3 9.22 85.65
Treatment group 4 7.83 87.82
Treatment group 5 7.15 88.88
Treatment group 6 9.15 85.76
Treatment group 7 7.22 88.77
Treatment group 8 8.15 87.32
Treatment group 9 19.36 69.88
Treatment group 10 22.22 65.43
Treatment group 11 33.15 48.42
Treatment group 12 16.83 73.81
Blank control CK 64.27 /
Note that: in this table, treatment groups 1 to 8 used the agents of examples 1 to 8, and treatment groups 9 to 12 used the agents of comparative examples 1 to 4, respectively; the blank group was administered clear water. The average was calculated after three replicates. And/represents no statistics.
Table 2 results of pesticide field efficacy test for controlling spodoptera frugiperda
Note that: in this table, treatment groups 1 to 8 used the agents of examples 1 to 8, and treatment groups 9 to 12 used the agents of comparative examples 1 to 4, respectively; the blank group was administered clear water. The average was calculated after three replicates. And/represents no statistics.
As can be seen from tables 1-2 and FIG. 1, the biocontrol agents prepared in examples 1-8 of the present invention have a good effect on the control of Spodoptera frugiperda.
The present invention is not limited to the above-mentioned embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present invention are intended to be included in the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims (5)

1. A biocontrol preparation is characterized by comprising entomopathogenic nematodes, sucrose, phyllostanol acetate and a nutrition aid; the entomopathogenic nematodes are the nematodes Steinernema carpocapsae; the content of the entomopathogenic nematodes in the biocontrol agent is 5700000 IJs-5900000 IJs; the content of the leaf alcohol acetate in the biocontrol agent is 1-1.3%; the mass ratio of the sucrose to the phyllostanol acetate is 9:1; the nutrition auxiliary agent comprises the following components in parts by mass: 5 parts of peptone, 5.5 parts of beef extract, 3 parts of agar powder, 4 parts of wheat flour, 4.5 parts of corn oil, 10 parts of corn leaf alcohol extract and 5.5 parts of yolk powder.
2. Use of the biocontrol formulation of claim 1 for controlling crop spodoptera frugiperda.
3. The use according to claim 2, wherein the crop is maize.
4. The use according to claim 3, wherein the control time is 6-8 leaf age of corn.
5. The use according to claim 2, wherein the biocontrol agent is applied in an amount to ensure that the amount of entomopathogenic nematodes is 1.5 x 10 9 IJs/mu-1.8X10 9 IJs/mu, and is diluted by 10 times with water before use.
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CN115769818A (en) * 2023-01-30 2023-03-10 华南农业大学 Application of (cis) -3-hexenol acetate in inducing spodoptera frugiperda to lay eggs and serving as spodoptera frugiperda attractant

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Publication number Priority date Publication date Assignee Title
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