CN1412162A - Preparation method of total saponin extract in notoginseng leaf - Google Patents
Preparation method of total saponin extract in notoginseng leaf Download PDFInfo
- Publication number
- CN1412162A CN1412162A CN 02144840 CN02144840A CN1412162A CN 1412162 A CN1412162 A CN 1412162A CN 02144840 CN02144840 CN 02144840 CN 02144840 A CN02144840 A CN 02144840A CN 1412162 A CN1412162 A CN 1412162A
- Authority
- CN
- China
- Prior art keywords
- preparation
- total saponin
- sanchi leaf
- resin column
- saponin extracts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The preparation method of notoginseng leaf total saponin extract includes the following steps: decocting notoginseng leaf by adding water, combining filtrates obtained by decocting notoginseng leaf for several times, concentracting, regulating pH value to 8-14, standing still and taking out supernatant fluid for stand-by; making the above-mentioned material pass through the macroporous adsorption resin column, eluting and collecting eluant, making eluant pass through resin column, recovering ethyl alcohol, concentrating, drying to obtain dried powder, refining the dried powder with water so as to obtain the invented notoginseng leaf total saponin extract whose purity can be up to above 85%.
Description
Technical field
The invention belongs to the extracts active ingredients field of crude drug.
Background technology
Sanchi Leaf is the dry cauline leaf of panax araliaceae plant Panax notoginseng (Burk.) F.H.chen, it contain with Radix Notoginseng in similar total saponins, content height in the flavones ingredient beguine that contains simultaneously, it is the ratio difference of monomer saponin in the total saponins, mostly be the dammarane type saponin(e of 20 (S)-protopanoxadiol types in the Sanchi Leaf greatly, Rb saponins content is higher, especially ginsenoside Rb
3The content height.And panoxadiol is suitable with triol type saponin content in the Radix Notoginseng.Folium Notoginseng total arasaponins has vasodilation equally, the effect of anticoagulant, the effect that also has anti-inflammatory, analgesia, anti-ageing, raise immunity simultaneously.
At present, the domestic colleague of the preparation method of saponin component also has report in the Sanchi Leaf, decocting or the extraction using alcohols of adopting more, extracting solution is with sherwood oil or ether defatting, and (or water liquid is concentrated into flowing soaking paste, the adding water-saturated n-butanol to water liquid with the water-saturated n-butanol extraction, heating is extracted, divide then and get n-butanol layer), propyl carbinol liquid reclaims solvent, promptly gets Folium Notoginseng total arasaponins.These methods have all adopted relatively large organic solvent, and are inflammable, explosive, the cost height, and the Folium Notoginseng total arasaponins powder appearance luster that makes is dark, and the net content of Folium Notoginseng total arasaponins is on the low side.
Summary of the invention
The invention provides the preparation method of total saponin extracts in a kind of Sanchi Leaf, is solvent with water, ethanol only, adopts adsorption resin method to prepare high purity saponin(e in the Sanchi Leaf, and the concrete technical scheme that the present invention takes is:
(1) the Sanchi Leaf boiling is 2～3 times, each 1～2 hour;
(2) filtrate with each time decoction gained merges, and concentrates;
(3) concentrated solution is regulated pH value to 8～14, placed, supernatant liquor is standby;
(4) supernatant liquor is crossed macroporous adsorptive resins (I post),, discarded washing lotion,, collect elutriant with 40%～100% ethanol elution with 0%～40% washing with alcohol;
(5) elutriant reclaims ethanol by resin column (II post), concentrates, and drying gets dry powder;
(6) dry powder is made with extra care with water, got the Folium Notoginseng total arasaponins extract, contain total saponins more than 85%.
The invention has the advantages that: be solvent with water and ethanol only in the preparation process, reduce and pollute that can reuse for several times after the used resin regeneration, cost is low, the Sanchi Leaf high purity saponin(e purity that makes can reach more than 85%.Total saponins can be used for cardiovascular and cerebrovascular diseases, pain, aging, reducing blood-fat, treatment of diseases such as immunologic hypofunction in the pseudo-ginseng that the present invention makes.
Embodiment
Embodiment one:
(1) gets 2 kilograms of Sanchi Leafs, boiling 2 times, each 2 hours;
(2) filtrate with twice decoction gained merges, and relative density is 1.11 when being concentrated into 50 ℃;
(3) concentrated solution is regulated pH value to 10, placed, supernatant liquor is standby;
(4) supernatant liquor is crossed macroporous adsorptive resins (I post),, discarded washing lotion,, collect elutriant with 60% ethanol elution with 10% washing with alcohol;
(5) elutriant reclaims ethanol by weakly anionic resin post (II post), concentrates, and drying gets dry powder;
(6) dry powder is made with extra care with water, got the Folium Notoginseng total arasaponins extract, it contains total saponins and contains more than 85%.
Embodiment two:
(1) gets 2 kilograms of Sanchi Leafs, boiling 3 times, each 1 hour;
(2) filtrate with three decoction gained merges, and relative density is 1.07 when being concentrated into 80 ℃;
(3) concentrated solution is regulated pH value to 11, placed, supernatant liquor is standby;
(4) supernatant liquor is crossed macroporous adsorptive resins (I post),, discarded washing lotion,, collect elutriant with 80% ethanol elution with 15% washing with alcohol;
(5) elutriant is passed through weakly anionic resin post (II post), reclaim ethanol, concentrate, drying gets dry powder;
(6) dry powder is made with extra care with water, got the Folium Notoginseng total arasaponins extract.
Embodiment three:
(1) gets 2 kilograms of Sanchi Leafs, boiling 3 times, each 2 hours;
(2) filtrate with three decoction gained merges, and relative density is 1.11 when being concentrated into 30 ℃;
(3) concentrated solution is regulated pH value to 13, placed, supernatant liquor is standby;
(4) supernatant liquor is crossed macroporous adsorptive resins (I post),, discarded washing lotion,, collect elutriant with 65% ethanol elution with 30% washing with alcohol;
(5) elutriant reclaims ethanol by weakly anionic resin post (II post), concentrates, and drying gets dry powder;
(6) dry powder is made with extra care with water, got the Folium Notoginseng total arasaponins extract.
Claims (5)
1, the preparation method of total saponin extracts in the Sanchi Leaf is characterized in that it comprises the steps:
(1) the Sanchi Leaf boiling is 2～3 times, each 1～2 hour;
(2) filtrate with each time decoction gained merges, and concentrates;
(3) concentrated solution is regulated pH value to 8～14, placed, supernatant liquor is standby;
(4) supernatant liquor is crossed macroporous adsorptive resins (I post),, discarded washing lotion,, collect elutriant with 40%～100% ethanol elution with 0%～40% washing with alcohol;
(5) elutriant reclaims ethanol by resin column (II post), concentrates, and drying gets dry powder;
(6) dry powder is made with extra care with water, got the Folium Notoginseng total arasaponins extract, contain total saponins more than 85%.
2, the preparation method of total saponin extracts in the Sanchi Leaf according to claim 1 is characterized in that: in (2) step during with solution concentration to 30 ℃～80 ℃ relative density be 1.01～1.20.
3, the preparation method of total saponin extracts in the Sanchi Leaf according to claim 1 is characterized in that: in (3) step, concentrated solution pH value is adjusted to 10.
4, the preparation method of total saponin extracts in the Sanchi Leaf according to claim 1 is characterized in that: the macroporous adsorptive resins of crossing in (4) step is D
21The model resin column earlier with water washing, continues with 80% ethanol elution.
5, the preparation method of total saponin extracts in the Sanchi Leaf according to claim 1 is characterized in that: the resin column of crossing in (5) step is a ZTC macropore decolorizing resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02144840 CN1412162A (en) | 2002-11-13 | 2002-11-13 | Preparation method of total saponin extract in notoginseng leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02144840 CN1412162A (en) | 2002-11-13 | 2002-11-13 | Preparation method of total saponin extract in notoginseng leaf |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1412162A true CN1412162A (en) | 2003-04-23 |
Family
ID=4750680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02144840 Pending CN1412162A (en) | 2002-11-13 | 2002-11-13 | Preparation method of total saponin extract in notoginseng leaf |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1412162A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1825860A1 (en) * | 2004-11-09 | 2007-08-29 | The Pharmaceutical Factory of Chengdu Hoist Inc., Ltd. | A panaxatriol saponins composition and its preparing method and the use |
| CN102178713A (en) * | 2011-04-13 | 2011-09-14 | 云南云科药业有限公司 | Panax notoginseng extract with antibacterial pharmacological activity as well as preparation method and application thereof |
| CN103462009A (en) * | 2013-09-04 | 2013-12-25 | 云南七丹药业股份有限公司 | Traditional Chinese medicine extract for assisting reduction of blood fat and preparation method thereof |
| CN107693559A (en) * | 2017-11-27 | 2018-02-16 | 贵州浩诚药业有限公司 | The preparation method of Notogineng Extract |
| CN109528671A (en) * | 2018-12-29 | 2019-03-29 | 云南金七制药有限公司 | Sanchi leaf drop pills of total saponin |
-
2002
- 2002-11-13 CN CN 02144840 patent/CN1412162A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1825860A1 (en) * | 2004-11-09 | 2007-08-29 | The Pharmaceutical Factory of Chengdu Hoist Inc., Ltd. | A panaxatriol saponins composition and its preparing method and the use |
| EP1825860A4 (en) * | 2004-11-09 | 2009-03-11 | Pharmaceutical Factory Of Chen | A panaxatriol saponins composition and its preparing method and the use |
| CN102178713A (en) * | 2011-04-13 | 2011-09-14 | 云南云科药业有限公司 | Panax notoginseng extract with antibacterial pharmacological activity as well as preparation method and application thereof |
| CN102178713B (en) * | 2011-04-13 | 2012-12-12 | 云南云科药业有限公司 | Panax notoginseng extract with antibacterial pharmacological activity as well as preparation method and application thereof |
| CN103462009A (en) * | 2013-09-04 | 2013-12-25 | 云南七丹药业股份有限公司 | Traditional Chinese medicine extract for assisting reduction of blood fat and preparation method thereof |
| CN107693559A (en) * | 2017-11-27 | 2018-02-16 | 贵州浩诚药业有限公司 | The preparation method of Notogineng Extract |
| CN109528671A (en) * | 2018-12-29 | 2019-03-29 | 云南金七制药有限公司 | Sanchi leaf drop pills of total saponin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104435401A (en) | Method for purifying general flavone from aboveground part of radix tetrastigme | |
| CN1865273A (en) | Method for extracting multiple liquorice flavone form liquorice | |
| CN100393709C (en) | Process for extracting tanshinone | |
| CN106892949A (en) | It is a kind of to extract the method for separating glycyrrhizic acid, glycyrrhiza total flavonoid simultaneously based on continuous chromatography technology | |
| CN108653355A (en) | A kind of Herba Centipedae extract preparation method with anti-inflammatory effect | |
| CN107126452A (en) | Gypenosides and preparation method thereof | |
| CN105213441A (en) | A kind of technique simultaneously preparing glycosides and Folium hydrangeae strigosae total polyphenols | |
| CN101134767A (en) | Highly effective abstraction method for preparation of high-purity ginko flavonoid | |
| CN1412162A (en) | Preparation method of total saponin extract in notoginseng leaf | |
| CN1414011A (en) | Extraction method of notoginsenoside | |
| CN1200916C (en) | Preparation method of total saponin and flavone extract in notoginseng leaf | |
| CN106366152B (en) | The method that asiaticosid is extracted from centella | |
| CN106380504B (en) | The method that madecassoside is extracted from centella | |
| CN1813834A (en) | Method for preparing total flavone extract of many prickle acanthopanax | |
| CN106420871A (en) | Preparation method of Panax notoginseng saponins rich in Fe and Fd and monomers Fe and Fd thereof | |
| CN1211378C (en) | Method for preparing ginkgo leaf extractive | |
| CN105294793A (en) | Separation method for naringin in aizoon stonecrop herb | |
| CN108272844A (en) | A kind of method that slender acanthopanax joins extraction Radix Et Caulis Acanthopanacis Senticosi total glucosides in oral solution and slender acanthopanax ginseng | |
| CN103690588A (en) | Production method for extracting refined gypenosides from gynostemma pentaphylla | |
| CN1102148C (en) | Preparation method of scutellaria root extract | |
| CN1869051B (en) | Preparation method of trialcohol group ginseng saponine and dialcohol group ginseng saponine | |
| CN1157414C (en) | Process for extracting and separating astragalus polyase and astragalus saponin | |
| CN107163616A (en) | A kind of method and its purification process for extracting walnut green husk pigment | |
| CN1869050A (en) | Method of preparing notoginseng saponine Fe and diol group ginseng saponine from netoginseng leaf | |
| CN101869605B (en) | Method for extracting and separating mulberry leaf flavone and alkaloid composite |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |