CN1813834A - Method for preparing total flavone extract of many prickle acanthopanax - Google Patents
Method for preparing total flavone extract of many prickle acanthopanax Download PDFInfo
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- CN1813834A CN1813834A CN 200610016616 CN200610016616A CN1813834A CN 1813834 A CN1813834 A CN 1813834A CN 200610016616 CN200610016616 CN 200610016616 CN 200610016616 A CN200610016616 A CN 200610016616A CN 1813834 A CN1813834 A CN 1813834A
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- acanthopanacis senticosi
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- folium acanthopanacis
- ethanol
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Abstract
The present invention relates to a manyprickle acanthopanax leaf total flavone extract preparation method. Said preparation method includes the following steps: using manyprickle acanthopanax leaf as raw material, removing impurity, cutting, adding ethyl alcohol to make reflux extraction in extraction tank for 2-4 times, every time is 1-3 hr, combining extracts, concentrating, filtering, adding the filtrate into polyamide column, using water to make elution, then using ethyl alcohol to make elution, recovering ethyl alcohol, concentrating and spray-drying so as to obtain the manyprickle acanthopanax leaf total flavone extract whose total flavone content is 50%-99%.
Description
Technical field
The invention belongs to the field of Chinese medicines, relate to the preparation method of Folium Acanthopanacis Senticosi total flavones extract.
Background technology
The extracting method that with the Radix Et Caulis Acanthopanacis Senticosi is all kinds of preparations of raw material is generally the water extract-alcohol precipitation extraction method, and the water of this method is put forward mode and compared with alcohol extracting method, and is low to the extraction ratio of flavone compound.What Song Shiyue etc. [Chinese patent, 11133176] with the Radix Et Caulis Acanthopanacis Senticosi were that the Radix Et Caulis Acanthopanacis Senticosi injection powder pin of raw material adopts is that the water extract-alcohol precipitation extraction method is extracted after the macroporous resin column separation method obtains Radix Et Caulis Acanthopanacis Senticosi extract.And macroporous adsorbent resin is because relatively poor to the selectivity of flavone compound, and the extractive total flavone content that obtains is not high.
Summary of the invention
The preparation method that the purpose of this invention is to provide Folium Acanthopanacis Senticosi total flavones extract.Preparation method of the present invention is the total flavones in the extraction separation Folium Acanthopanacis Senticosi effectively, general flavone content height in the extract, perfect technology, rationally.
The preparation method of a kind of Folium Acanthopanacis Senticosi total flavones extract of the present invention is: with Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 40%-95% alcohol reflux 2-4 time, each 1-3 hour, add alcoholic acid volume L: Folium Acanthopanacis Senticosi weight Kg was 6-12: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with the 10%-30% ethanol elution again to effluent, discard above-mentioned eluent, again with the 40%-70% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.In hyperin, general flavone content is 50%-99%.
Folium Acanthopanacis Senticosi total flavones extract can be treated apoplexy.Total flavones in the preparation method extraction separation Folium Acanthopanacis Senticosi of the present invention can be made any solid dosage forms with existing Chinese drug preparation technique and process, comprises capsule, tablet, powder or pill etc.
The specific embodiment
Embodiment 1:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 40% alcohol reflux 4 times, each 1 hour, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 6: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with 10% ethanol elution again to effluent, discard above-mentioned eluent, again with 40% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 2:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 50% alcohol reflux 2 times, each 3 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 11: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with 20% ethanol elution again to effluent, discard above-mentioned eluent, again with 50% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 3:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 60% alcohol reflux 2 times, each 2.5 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 12: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with 30% ethanol elution again to effluent, discard above-mentioned eluent, again with 60% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 4:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 70% alcohol reflux 3 times, each 1.5 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 9: 1 at every turn, extracting solution merges, and concentrates, and filters, add in the polyamide column of having handled well, closely colourless with water elution, faint yellow with 10%, 20% ethanol elution again to effluent to effluent, discard above-mentioned eluent, with 70% ethanol elution, it is faint yellow to effluent to collect eluent again, reclaims ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 5:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 80% alcohol reflux 2 times, each 2 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 10: 1 at every turn, extracting solution merges, and concentrates, and filters, add in the polyamide column of having handled well, closely colourless with water elution, faint yellow with 10%, 30% ethanol elution again to effluent to effluent, discard above-mentioned eluent, with 55% ethanol elution, it is faint yellow to effluent to collect eluent again, reclaims ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Embodiment 6:
With the 5kg Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 95% alcohol reflux 3 times, each 2 hours, add ethanol volume L: Folium Acanthopanacis Senticosi weight Kg is 8: 1 at every turn, extracting solution merges, concentrate, filter, add in the polyamide column of having handled well, closely colourless with water elution to effluent, faint yellow with 25% ethanol elution again to effluent, discard above-mentioned eluent, again with 65% ethanol elution, it is faint yellow to effluent to collect eluent, reclaim ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Claims (1)
1. the preparation method of a Folium Acanthopanacis Senticosi total flavones extract, it is characterized in that: with Folium Acanthopanacis Senticosi through remove impurity, cataclasm, place extraction pot, add 40%-95% alcohol reflux 2-4 time, each 1-3 hour, add alcoholic acid volume L: Folium Acanthopanacis Senticosi weight Kg is 6-12 at every turn: 1, and extracting solution merges, and concentrates, filter, add in the polyamide column of having handled well, closely colourless with water elution, faint yellow with the 10%-30% ethanol elution again to effluent to effluent, discard above-mentioned eluent, with the 40%-70% ethanol elution, it is faint yellow to effluent to collect eluent again, reclaims ethanol and concentrated, spray drying promptly gets Folium Acanthopanacis Senticosi total flavones extract of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016616 CN1813834A (en) | 2006-03-03 | 2006-03-03 | Method for preparing total flavone extract of many prickle acanthopanax |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016616 CN1813834A (en) | 2006-03-03 | 2006-03-03 | Method for preparing total flavone extract of many prickle acanthopanax |
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| CN1813834A true CN1813834A (en) | 2006-08-09 |
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| CN 200610016616 Pending CN1813834A (en) | 2006-03-03 | 2006-03-03 | Method for preparing total flavone extract of many prickle acanthopanax |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100446779C (en) * | 2007-06-20 | 2008-12-31 | 黑龙江省珍宝岛制药有限公司 | Method for preparing acanthopanax senticousus extracting liquid/extraction |
| CN102579531A (en) * | 2012-02-20 | 2012-07-18 | 山东大学威海分校 | Acanthopanax leaf extract having antioxidation |
| CN102977164A (en) * | 2012-11-05 | 2013-03-20 | 河南牧翔动物药业有限公司 | Enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms |
| CN104069312A (en) * | 2013-03-29 | 2014-10-01 | 天津药物研究院 | Traditional Chinese medicine composition for treating stroke, and preparation method, pharmaceutical preparation and application thereof |
| CN105476962A (en) * | 2015-12-04 | 2016-04-13 | 中国科学院长春应用化学研究所 | Acanthopanax leave flavone lipidosome and preparation method thereof |
| CN106309523A (en) * | 2015-11-01 | 2017-01-11 | 长沙博海生物科技有限公司 | Total flavonoid extract of acanthopanax senticosus harms, as well as preparation method and application thereof |
-
2006
- 2006-03-03 CN CN 200610016616 patent/CN1813834A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100446779C (en) * | 2007-06-20 | 2008-12-31 | 黑龙江省珍宝岛制药有限公司 | Method for preparing acanthopanax senticousus extracting liquid/extraction |
| CN102579531A (en) * | 2012-02-20 | 2012-07-18 | 山东大学威海分校 | Acanthopanax leaf extract having antioxidation |
| CN102977164A (en) * | 2012-11-05 | 2013-03-20 | 河南牧翔动物药业有限公司 | Enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms |
| CN102977164B (en) * | 2012-11-05 | 2015-09-30 | 河南牧翔动物药业有限公司 | The enzyme assisted extraction process of Quercetin 3-galactoside in a kind of Folium Acanthopanacis Senticosi |
| CN104069312A (en) * | 2013-03-29 | 2014-10-01 | 天津药物研究院 | Traditional Chinese medicine composition for treating stroke, and preparation method, pharmaceutical preparation and application thereof |
| CN106309523A (en) * | 2015-11-01 | 2017-01-11 | 长沙博海生物科技有限公司 | Total flavonoid extract of acanthopanax senticosus harms, as well as preparation method and application thereof |
| CN105476962A (en) * | 2015-12-04 | 2016-04-13 | 中国科学院长春应用化学研究所 | Acanthopanax leave flavone lipidosome and preparation method thereof |
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